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1.
Int J Biol Macromol ; : 123500, 2023 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-36736520

RESUMO

Lycium barbarum polysaccharide (LBP) is the main active component of Lycium barbarum (L. barbarum), which has important medicinal and nutritional value. However, the effect of LBP treatment on Luciobarbus capito (L. capito) still remains unknown. Given this, the current work aims to probe the underlying effect of different levels of LBP treatment (i.e. 0.10, 0.50 and 1.00 g/L) on L. capito in the context of enzymatic activity analysis, histological observations and gut microbiota analysis. Compared with control group, the activities of hepatic antioxidant enzymes, intestinal digestive enzymes and hepatic immune enzyme were found to be significantly increased after 0.10 g/L LBP and 0.50 g/L LBP treatment (P < 0.05). This result indicated that moderate levels of LBP treatment could dramatically enhance the immunity and antioxidant capacity of L. capito. Furthermore, the compositional structures of the gut microbiota in L. capito were found to be greatly shaped after LBP treatment, whereas the diversity and abundance of the gut microbiota were only found to be slightly changed (P > 0.05). No significant changes were screened in the morphologic structures of gut constructions. This work would provide theoretical and experimental basis for future application of LBP as supplement in the culture process of the farmed fish.

2.
Artigo em Inglês | MEDLINE | ID: mdl-36717043

RESUMO

Insecticide resistance has become an increasingly serious challenge for agriculture in the world. To reveal the mechanisms of insecticide resistance, majority of studies have been carried out on the insensitivity of insecticide targets and the metabolism of insecticides. However, the mechanism of the insecticide penetration resistance in insects remains unclear. This study aimed to reveal the mechanism underlying the penetration resistance of Drosophila larvae to insecticide avermectin (AVM). Levels of intercellular junction proteins (IJPs) in the larvae were determined by Western blotting analysis and immunofluorescence assay. The result showed that the expression of IJPs septate junction and adherens junction proteins increased in the AVM-resistant insects compared with those in the AVM-susceptible ones, and the upregulation of the IJPs was mediated by the activation of protein kinase C (PKC) pathway. That AVM induced the activation of PKC was found not only in the Drosophila larvae but also in Drosophila S2 cells. These findings revealed that AVM could activate PKC pathway in Drosophila larvae, which mediated the upregulation of the IJPs and then led to the resistance to AVM, suggesting that the chemicals that can disrupt PKC activation may potentially be used to circumvent the resistance to AVM in insects.

3.
J Colloid Interface Sci ; 637: 193-206, 2023 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-36701865

RESUMO

Nucleus targeting is tremendously important in cancer therapy. Cationic carbon dots (CCDs) are potential nanoparticles which might enter cells and penetrate nuclear membranes. Although some CCDs have been investigated in nucleus targeting and applied in nuclear imaging, the CCDs derived from drugs, that are able to target the nucleus, bind with DNA and inhibit the growth of cancer cells have not been reported. In this project, 1, 2, 4, 5-benzenetetramine (Y15, a focal adhesion kinase inhibitor) derived cationic carbon dots (Y15-CDs) were prepared via a hydrothermal approach utilizing Y15, folic acid and 1,2-ethylenediamine as precursors. Based on the structural, optical, and morphologic characterizations, Y15-CDs possess rich amine groups and nitrogen in structure, an excitation-dependent photoluminescence emission, and a small particle size of 2 to 4 nm. The DNA binding experiments conducted through agarose gel electrophoresis, UV-vis absorption, fluorescence emission, and circular dichroism spectroscopies, prove that Y15-CDs might bind with DNA via electrostatic interactions and partially intercalative binding modes. In addition, the cell imaging and cytotoxicity studies in human foreskin fibroblasts (HFF), prostate cancer (PC3) and osteosarcoma cells (U2OS) indicate the nucleus targeting and anticancer abilities of Y15-CDs. Most interestingly, Y15-CDs exhibit a higher cytotoxicity to cancer cells (PC3 and U2OS) than to normal cells (HFF), inferring that Y15-CDs might be potentially applied in cancer therapy.

4.
Antioxidants (Basel) ; 12(1)2023 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-36671059

RESUMO

The rise in water temperature caused by global warming is seriously threatening the development of aquatic animals. However, the physiological response mechanism behind the adverse effects of thermal conditions on L. capito remains unclear. In this study, we investigated the physiological responses of L. capito exposed to thermal stress via biochemical analyses and intestinal microbiota and liver LC-MS metabolomics. The results show that the superoxide dismutase (SOD) and catalase (CAT) activities significantly decrease, while the malondialdehyde (MDA) content, aspartate aminotransferase (AST), acid phosphatase (ACP), alanine aminotransferase (ALT), and albumin (ALB) activities, and glucose (Glu) level significantly increase. Obvious variations in the intestinal microbiota were observed after stress exposure, with increased levels of Proteobacteria and Bacteroidota and decreased levels of Firmicutes, Fusobacteriota, and Actinobacteriota, while levels of several genera of pathogenic bacteria increased. Liver metabolomic analysis showed that stress exposure disturbed metabolic processes, especially of amino acids and lipids. The results of this study indicated that thermal stress caused oxidative stress, disturbed blood biological functioning and intestinal microbiota balance, and damaged amino acids and lipids metabolism of liver in L. capito.

6.
J Adv Res ; 43: 205-218, 2023 01.
Artigo em Inglês | MEDLINE | ID: mdl-36585109

RESUMO

Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease characterized by oxidative stress that triggers motor neurons loss in the brain and spinal cord. However, the mechanisms underlying the exact role of oxidative stress in ALS-associated neural degeneration are not definitively established. Oxidative stress-generated phospholipid peroxides are known to have extensive physiological and pathological consequences to tissues. Here, we discovered that the deficiency of glutathione peroxidase 4 (GPX4), an essential antioxidant peroxidase, led to the accumulation of phospholipid peroxides and resulted in a loss of motor neurons in spinal cords of ALS mice. Mutant human SOD1G93A transgenic mice were intrathecally injected with neuron-targeted adeno-associated virus (AAV) expressing GPX4 (GPX4-AAV) or phospholipid peroxidation inhibitor, ferrostatin-1. The results showed that impaired motor performance and neural loss induced by SOD1G93A toxicity in the lumbar spine were substantially alleviated by ferrostatin-1 treatment and AAV-mediated GPX4 delivery. In addition, the denervation of neuron-muscle junction and spinal atrophy in ALS mice were rescued by neural GPX4 overexpression, suggesting that GPX4 is essential for the motor neural maintenance and function. In comparison, conditional knockdown of Gpx4 in the spinal cords of Gpx4fl/fl mice triggered an obvious increase of phospholipid peroxides and the occurrence of ALS-like motor phenotype. Altogether, our findings underscore the importance of GPX4 in maintaining phospholipid redox homeostasis in the spinal cord and presents GPX4 as an attractive therapeutic target for ALS treatment.


Assuntos
Esclerose Amiotrófica Lateral , Doenças Neurodegenerativas , Camundongos , Humanos , Animais , Esclerose Amiotrófica Lateral/genética , Esclerose Amiotrófica Lateral/patologia , Superóxido Dismutase-1/genética , Doenças Neurodegenerativas/patologia , Superóxido Dismutase/genética , Neurônios Motores/patologia , Camundongos Transgênicos , Glutationa Peroxidase/genética , Peróxidos
7.
Oxid Med Cell Longev ; 2022: 7223353, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36457727

RESUMO

Photobiomodulation (PBM) has been repeatedly reported to play a major role in the regulation of osteoblast proliferation and mineralization. Autophagy is closely associated with various pathophysiological processes in osteoblasts, while its role in oxidative stress is even more critical. However, there is still no clear understanding of the mechanism of the role of autophagy in the regulation of osteoblast mineralization and apoptosis under oxidative stress by PBM. It was designed to investigate the impact of 808 nm PBM on autophagy and apoptosis in mouse preosteoblast MC3T3-E1 treated with hydrogen peroxide (H2O2) through PI3K/AKT/mTOR pathway. PBM could inhibit MC3T3-E1 cell apoptosis under oxidative stress and promote the expression of osteogenic proteins, while enhancing the level of autophagy. In contrast, 3-methyladenine (3-MA) inhibited the expression of osteoblast autophagy under oxidative stress conditions, increased apoptosis, and plus counteracted the effect of PBM on osteoblasts. We also found that PBM suppressed the activated PI3K/AKT/mTOR pathway during oxidative stress and induced autophagy in osteoblasts. PBM promoted autophagy of MC3T3 cells and was further blocked by 740 Y-P, which reversed the effect of PBM on MC3T3 cells with H2O2. In conclusion, PBM promotes autophagy and improves the level of osteogenesis under oxidative stress by inhibiting the PI3K/AKT/mTOR pathway. Our results can lay the foundation for the clinical usage of PBM in the treatment of osteoporosis.


Assuntos
Calcinose , Peróxido de Hidrogênio , Animais , Camundongos , Peróxido de Hidrogênio/toxicidade , Fosfatidilinositol 3-Quinases , Proteínas Proto-Oncogênicas c-akt , Estresse Oxidativo , Serina-Treonina Quinases TOR , Autofagia
8.
BMC Cardiovasc Disord ; 22(1): 528, 2022 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-36474152

RESUMO

INTRODUCTION: Acute heart failure is a serious condition. Atrial fibrillation is the most frequent arrhythmia in patients with acute heart failure. The occurrence of atrial fibrillation in heart failure patients worsens their prognosis and leads to a substantial increase in treatment costs. There is no tool that can effectively predict the onset of atrial fibrillation in patients with acute heart failure in the ICU currently. MATERIALS AND METHODS: We retrospectively analyzed the MIMIC-IV database of patients admitted to the intensive care unit (ICU) for acute heart failure and who were initially sinus rhythm. Data on demographics, comorbidities, laboratory findings, vital signs, and treatment were extracted. The cohort was divided into a training set and a validation set. Variables selected by LASSO regression and multivariate logistic regression in the training set were used to develop a model for predicting the occurrence of atrial fibrillation in acute heart failure in the ICU. A nomogram was drawn and an online calculator was developed. The discrimination and calibration of the model was evaluated. The performance of the model was tested using the validation set. RESULTS: This study included 2342 patients with acute heart failure, 646 of whom developed atrial fibrillation during their ICU stay. Using LASSO and multiple logistic regression, we selected six significant variables: age, prothrombin time, heart rate, use of vasoactive drugs within 24 h, Sequential Organ Failure Assessment (SOFA) score, and Acute Physiology Score (APS) III. The C-index of the model was 0.700 (95% CI 0.672-0.727) and 0.682 (95% CI 0.639-0.725) in the training and validation sets, respectively. The calibration curves also performed well in both sets. CONCLUSION: We developed a simple and effective model for predicting atrial fibrillation in patients with acute heart failure in the ICU.

9.
Cell Death Dis ; 13(12): 1040, 2022 12 14.
Artigo em Inglês | MEDLINE | ID: mdl-36517470

RESUMO

Ferroptosis is a recently-defined tumor suppression mechanism, but the sensitivity of many tumorigenic cells to ferroptosis is limited by their deficient expression of acyl-CoA synthetase long-chain family member 4 (ACSL4). Here, we report the discovery of a photosensitizer, namely TPCI, which can evoke ACSL4-independent ferroptosis of cancer cells in photodynamic therapy. Through co-localization with 12-lipoxygenase (ALOX12) in multiple subcellular organelles, TPCI activates ALOX12 to generate lipid reactive oxygen species in large quantity and trigger cell ferroptosis. Intriguingly, confining TPCI exclusively in lysosomes switches the cell death from ferroptosis to apoptosis. More strikingly, the ferroptosis mediated by TPCI-induced ALOX12 activation does not require the participation of ACSL4. Therefore, our study identifies TPCI as the first ALOX12 activator to induce ferroptosis independent of ACSL4, which renders a viable therapeutic approach on the basis of distinct ferroptosis of cancer cells, regardless their ACSL4 expressions.


Assuntos
Ferroptose , Fármacos Fotossensibilizantes/farmacologia , Coenzima A Ligases/metabolismo , Apoptose , Organelas/metabolismo
10.
Int J Mol Sci ; 23(23)2022 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-36498848

RESUMO

Grafting is a technique that is widely used in citrus production. Graft incompatibility often occurs in the orchard. Inarching can effectively improve the vigor of incompatible grafts, but its mechanism remains poorly understood. Our previous studies investigated the scion-rootstock interaction of citrus and highlighted the role of hormonal balance and genes in abscisic acid biosynthesis regulation. To further elucidate the mechanism of inarched grafts rejuvenation, Hm/Pt combination (Citrus maxima (Burm.) Merrill cv. 'Hongmian miyou' grafted onto Poncirus trifoliata) were inarched with 'Pujiang Xiangcheng' (a novel citrus rootstock cultivar recently selected from wild Citrus junos populations), and comprehensive analysis was performed to compare the inarched grafts and controls. Compared with incompatible grafts, the results revealed that inarching could recover the leaf metabolism balance, including reducing starch content, increasing chlorophyll content and restoring the cell structure. Additionally, our results corroborated that hormonal balance and hormone-related genes played a central role in inarching compatibility. Furthermore, the roles of Hsf4, ERF1, NCED3 and PYL were highlighted, and a model for explaining inarched grafts recovery invigoration was proposed. This study shed light on the mechanism of inarching regulation tree vigor and offered deep insights into the scion-rootstock interaction in citrus.


Assuntos
Citrus , Citrus/genética , Citrus/metabolismo , Perfilação da Expressão Gênica , Transcriptoma , Folhas de Planta/metabolismo
11.
Immunobiology ; 227(6): 152300, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36356459

RESUMO

AIMS: Dysregulated interferon regulatory factor 8 (IRF8) mediated inducible nitric oxide synthase (iNOS) transcription is crucial to the pathogenesis of several inflammatory disorders. However, the molecular mechanism that control the transcription activity of IRF8 in the regulation of iNOS is not fully elucidated. This study is undertaken to determine whether SIRT1 impacts IRF8 acetylation level in the macrophages. MAIN METHODS: The silver stain, mass spectrum, bone marrow-derived monocytes differentiation, lentiviral transduction, immunoprecipitation and chromatin immunoprecipitation assay were used to investigate the relationship between IRF8 and SIRT1. KEY FINDINGS: We demonstrate that deacetylation of IRF8 is induced by lipopolysaccharide (LPS) and suppresses iNOS expression. Macrophages expressing acetylation-defective iNOS are highly septic upon transfer to macrophages cleaned up mice. Mechanistically, deacetylation IRF8 facilitates the binding of silent information regulator 1 (SIRT1) to the iNOS promoter and restricts iNOS transcription. The expression of iNOS was enhanced in the macrophages from SIRT1 conditional knockout mice and the progression of sepsis is more serious. SIGNIFICANCE: The discovery of the IRF8-SIRT1 interaction that governs iNOS expression may exploit new therapeutic strategies for inflammatory disorders.


Assuntos
Macrófagos , Sirtuína 1 , Camundongos , Animais , Óxido Nítrico Sintase Tipo II/genética , Óxido Nítrico Sintase Tipo II/metabolismo , Sirtuína 1/genética , Sirtuína 1/metabolismo , Macrófagos/metabolismo , Fatores Reguladores de Interferon/genética , Fatores Reguladores de Interferon/metabolismo , Inflamação/metabolismo , Lipopolissacarídeos/metabolismo , Camundongos Knockout
12.
Nat Commun ; 13(1): 6835, 2022 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-36369424

RESUMO

Glioblastoma multiforme (GBM) is one of the most fatal malignancies due to the existence of blood-brain barrier (BBB) and the difficulty to maintain an effective drug accumulation in deep GBM lesions. Here we present a biomimetic nanogel system that can be precisely activated by near infrared (NIR) irradiation to achieve BBB crossing and deep tumor penetration of drugs. Synthesized by crosslinking pullulan and poly(deca-4,6-diynedioic acid) (PDDA) and loaded with temozolomide and indocyanine green (ICG), the nanogels are inert to endogenous oxidative conditions but can be selectively disintegrated by ICG-generated reactive oxygen species upon NIR irradiation. Camouflaging the nanogels with apolipoprotein E peptide-decorated erythrocyte membrane further allows prolonged blood circulation and active tumor targeting. The precisely controlled NIR irradiation on tumor lesions excites ICG and deforms the cumulated nanogels to trigger burst drug release for facilitated BBB permeation and infiltration into distal tumor cells. These NIR-activatable biomimetic nanogels suppress the tumor growth in orthotopic GBM and GBM stem cells-bearing mouse models with significantly extended survival.


Assuntos
Glioblastoma , Camundongos , Animais , Glioblastoma/tratamento farmacológico , Glioblastoma/patologia , Nanogéis , Biomimética , Temozolomida , Verde de Indocianina , Linhagem Celular Tumoral
13.
Medicine (Baltimore) ; 101(43): e31338, 2022 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-36316923

RESUMO

Rheumatic diseases have been reported to sometimes involve the pituitary gland. This study aims to characterize the clinical features and outcomes of patients with rheumatic disease-associated hypophysitis. We used the electronic medical record system in our hospital to identify nine patients with pituitary involvement in rheumatoid disease. We summarized the clinical characteristics, radiographic findings, treatments, and clinical outcomes of the 9 patients. We also performed a systematic literature review of systemic lupus erythematosus (SLE) cases with pituitary involvement published in PubMed and Wanfang databases from 1995 to 2021, and eight patients with complete information were selected. In the nine-patient cohort, the median age was 54 years, and the spectrum of rheumatic diseases included immunoglobulin G4-related disease (IgG4RD) (4/9), SLE (2/9), vasculitis (2/9), and Sjögren syndrome (SS) (1/9). All patients had pituitary abnormalities on radiological assessment, 6 developed diabetes insipidus (DI), and 8 presented with anterior pituitary hormone deficiencies in the disease duration. All the patients had multisystem involvement. As compared to hypophysitis with IgG4RD (IgG4-H), the age at onset of hypophysitis with SLE (SLE-H) patients was younger [(30.4 ± 16.4) years vs. (56.0 ± 0.8) years] and the disease duration was shorter [(14.0 ± 17.5) months vs. (71.0 ± 60.9) months] (P < .05). All patients were managed with glucocorticoids (GC) in combination with another immunosuppressant, and the majority of patients improved within 4 months. Six patients achieved disease remission while four required at least one hormone replacement therapy. Hypophysitis is a rare complication secondary to a variety of various rheumatic diseases that can occur at any stage. GC combined with additional immunosuppressants could improve patients' symptoms; however some patients also required long-term hormone replacement therapy in pituitary disorders.


Assuntos
Hipofisite Autoimune , Doenças do Colágeno , Hipofisite , Hipopituitarismo , Lúpus Eritematoso Sistêmico , Doenças da Hipófise , Doenças Reumáticas , Humanos , Pessoa de Meia-Idade , Adolescente , Adulto Jovem , Adulto , Hipofisite/complicações , Doenças da Hipófise/complicações , Doenças da Hipófise/diagnóstico , Hipopituitarismo/etiologia , Hipófise/diagnóstico por imagem , Glucocorticoides/uso terapêutico , Doenças Reumáticas/complicações , Doenças Reumáticas/tratamento farmacológico , Imunossupressores/uso terapêutico , Doenças do Colágeno/tratamento farmacológico , Lúpus Eritematoso Sistêmico/complicações , Lúpus Eritematoso Sistêmico/tratamento farmacológico , Hipofisite Autoimune/complicações , Hipofisite Autoimune/diagnóstico , Hipofisite Autoimune/tratamento farmacológico
14.
Chemistry ; 2022 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-36449331

RESUMO

Conjugated polymers with high charge mobilities have drawn increasing attention in organic field-effect transistors (OFETs) in recent years. However, OFETs of conjugated polymers with high mobility and good device stability remain a challenge. In this article, we report a hyperbranched polymer approach to improve the charge mobility and device stability. Three hyperbranched diketopyrrolopyrrole-based polymers were designed and synthesized via linear alkyl side-chain linkers. The results show that 2D topological hyperbranched polymers form stable thin film microstructures, and thus improve the device stability, since the conjugated moiety is interconnected by linear alkyl chain. Besides, the incorporation of linear alkyl chain instead of branching alkyl one reduce steric hindrance, and improve the microstructure ordering as well as the charge mobility. Bar-coated OFETs result demonstrates that the devices mobilities and operational stabilities (bias stability and bending resistance) are both improved. All these indicate that hyperbranched polymer is a potential candidate for future application.

15.
Artigo em Inglês | MEDLINE | ID: mdl-36301911

RESUMO

Two-dimensional (2D) nanomaterials hold great potential for cancer theranostic applications, yet their clinical translation faces great challenges of high toxicity and limited therapeutic/diagnostic modality. Here, we have created a kind of symbiotic 2D carbon-2D clay nanohybrids, which are composed of a novel 2D carbon nanomaterial (carbon nanochips, or CNC), prepared by carbonizing a conjugated polymer polydiiodobutadiyne, and a 2D layered aluminosilicate clay mineral montmorillonite (MMT). Intriguingly, with the formation of the nanohybrids, MMT can help the dispersion of CNC, while CNC can significantly reduce the hemolysis and toxicity of MMT. The symbiotic combination of CNC and MMT also leads to a synergistic anti-cancer theranostic effect. CNC has a strong absorption and high photothermal conversion efficiency in the second near-infrared region (NIR-II, 1000-1700 nm), while MMT contains Fe3+ that can facilitate the generation of reactive oxygen species from highly expressed H2O2 in tumor microenvironment. The nanohybrids not only enable a synergy of photothermal therapy and chemodynamic therapy to suppress the extremely rapid growth of RM1 tumors in mice but also allow for dual photoacoustic and magnetic imaging to guide the drug delivery and NIR-II irradiation execution, hence establishing a highly efficient and biosafe "all-in-one" theranostic platform for precision nanomedicine.

16.
Histol Histopathol ; : 18537, 2022 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-36269039

RESUMO

Gastric cancer (GC) triggers a great number of deaths worldwide. Although great efforts have been made in treating this cancer, GC patients' survival rate remains unsatisfactory. An increasing amount of evidence indicates that miR-29c-3p inhibits cancer progression. However, the mechanism of miR-29c-3p in GC remains to be fully defined. Hence, this work aimed to analyze the underlying mechanism of miR-29c-3p in GC. Outcomes showed marked downregulation of miR-29c-3p in GC tissue and cell lines. Functional experiments exhibited that miR-29c-3p repressed GC cell malignant behaviors. Moreover, bioinformatics analysis and dual-luciferase reporter gene detection indicated that MEST was targeted by miR-29c-3p. Rescue assay further proved that MEST participated in functions of miR-29c-3p in GC. To sum up, miR-29c-3p/MEST signaling pathway suppressed formation of malignant phenotypes of GC, and targeting the signaling pathway may be a new method for treating GC.

17.
Cell Death Dis ; 13(10): 862, 2022 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-36220823

RESUMO

The mouse vaginal epithelium cyclically exhibits cell proliferation and differentiation in response to estrogen. Estrogen acts as an activator of mTOR signaling but its role in vaginal epithelial homeostasis is unknown. We analyzed reproductive tract-specific Rptor or Rictor conditional knockout mice to reveal the role of mTOR signaling in estrogen-dependent vaginal epithelial cell proliferation and differentiation. Loss of Rptor but not Rictor in the vagina resulted in an aberrant proliferation of epithelial cells and failure of keratinized differentiation. As gene expression analysis indicated, several estrogen-mediated genes, including Pgr and Ereg (EGF-like growth factor) were not induced by estrogen in Rptor cKO mouse vagina. Moreover, supplementation of EREG could activate the proliferation and survival of vaginal epithelial cells through YAP1 in the absence of Rptor. Thus, mTORC1 signaling integrates estrogen and growth factor signaling to mediate vaginal epithelial cell proliferation and differentiation, providing new insights into vaginal atrophy treatment for post-menopausal women.


Assuntos
Fator de Crescimento Epidérmico , Estrogênios , Animais , Proliferação de Células , Fator de Crescimento Epidérmico/metabolismo , Células Epiteliais/metabolismo , Estrogênios/metabolismo , Estrogênios/farmacologia , Feminino , Alvo Mecanístico do Complexo 1 de Rapamicina/genética , Alvo Mecanístico do Complexo 1 de Rapamicina/metabolismo , Camundongos , Camundongos Knockout , Proteína Regulatória Associada a mTOR/metabolismo , Transdução de Sinais , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo , Vagina/metabolismo
18.
Front Microbiol ; 13: 969722, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36081787

RESUMO

The toxicity of ammonia nitrogen (AN) has always caused severe harm to aquatic animals in intensive aquaculture conditions, especially in saline-alkali aquaculture waters. The application of AN removal bacteria is a safe and effective method for controlling the AN concentration in aquaculture water through direct conversion to bacterial protein. However, there is still a lack of AN removal bacteria that are appropriate for saline-alkali aquaculture conditions. In this study, three AN removal strains, namely, Bacillus idriensis CT-WN-B3, Bacillus australimaris CT-WL5-10, and Pseudomonas oleovorans CT-WL5-6, were screened out under alkaline conditions from the alkali-tolerant strains distributed in carbonate saline-alkali soil and water environments in Northeast China. Under different pH (8.0-9.0), salinities (10-30 g/L NaCl), alkalinities (10-30 mmol/L NaHCO3), and AN concentrations (1-3 mg/L), corresponding to the actual conditions of saline-alkali aquaculture waters, the AN removal rates and relative characteristics of these strains were analyzed. The results showed that all of the three strains were efficient on AN removal under various conditions, and the highest removal rate reached up to 3 × 10-13 mg/cfu/h. Both CT-WL5-10 and CT-WL5-6 were most efficient under pH 9.0 with 3 mg/L initial AN, while pH 8.5 with 2 mg/L AN was the best fit for CT-WN-B3. In 96-h pure incubation of these strains in alkali media, approximately 90% AN was removed, and pH values were decreased by 2.0 units within 12 h accompanied by the growth of the strains. In addition, salinity and alkalinity slightly disturbed the removal rates of CT-WL5-10 and CT-WL5-6, but there were at least 65% AN removed by them within 24 h. These results indicated that all three strains have good application prospect in saline-alkali aquaculture waters.

19.
Psychon Bull Rev ; 2022 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-36109421

RESUMO

Recent studies have found that making judgments of learning (JOLs) for verbal materials changes memory itself, a form of reactivity effect on memory. The current study explores the reactivity effect on visual (image) memory and tests the potential role of enhanced learning engagement in this effect. Experiment 1 employed object image pairs as stimuli and observed a positive reactivity effect on memory for visual details. Experiment 2 conceptually replicated this positive reactivity effect using pairs of scene images. Experiment 3 introduced mind wandering (MW) probes to measure participants' attentional state (learning engagement) and observed that making JOLs significantly reduced MW. More importantly, reduced MW mediated the reactivity effect. Lastly, Experiment 4 found that a manipulation that heightened learning motivation decreased the reactivity effect. Overall, the current study provides the first demonstration of the reactivity effect on visual memory, as well as support for the enhanced learning engagement explanation. Practical implications are discussed.

20.
Nanoscale Adv ; 4(13): 2893-2901, 2022 Jun 28.
Artigo em Inglês | MEDLINE | ID: mdl-36132003

RESUMO

The morphology of interfacial nanobubbles (INBs) is a crucial but controversial topic in nanobubble research. We carried out atomistic molecular dynamics (MD) simulations to comprehensively study the morphology of INBs controlled by several determinant factors, including gas species, surface wettability, and bubble size. The simulations show that H2, O2 and N2 can all form stable INBs, with the contact angles (CAs, on the liquid side) following the order CA(H2) < CA(N2) < CA(O2), while CO2 prefers to form a gas film (pancake) structure on the substrate. The CA of INBs demonstrates a linear relation with the strength of interfacial interaction; however, a limited bubble CA of ∼25° is found on superhydrophilic surfaces. The high gas density and high internal pressure of the INBs are further confirmed, accompanied by strong interfacial gas enrichment (IGE) behavior. The morphology study of differently sized INBs shows that the internal density of the gas is drastically decreased with the bubble size at the initial stage of bubble nucleation, while the CA remains almost constant. Based on the simulation results, a modified Young's equation is presented for describing the extraordinary morphology of INBs.

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