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1.
J Affect Disord ; 260: 490-497, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31539685

RESUMO

BACKGROUND: Early improvement (EI) following treatment with antidepressants is a widely reported predictor to the treatment response. This study aimed to identify the resting-state functional connectivity (rs-FC) and its related clinical features that link the treatment response at the time of EI. METHODS: This study included 23 first-episode treatment-naive patients with MDD. After 2 weeks of antidepressant treatment, these patients received 3.0 Tesla resting-state functional magnetic resonance imaging scanning and were subgrouped into an EI group (N = 13) and a non-EI group (N = 10). Using the anterior insula (rAI) as a seed region, this study identified the rs-FC that were associated with both EI and the treatment response at week 12, and further tested the associations of the identified rs-FC with either the clinical features or the early symptom improvement. RESULTS: Rs-FC between rAI and the left dorsolateral prefrontal cortex (dlPFC) was associated with EI (t21 = -6.091, p = 0.022 after FDR correction for multiple comparisons). This rs-FC was also associated with an interaction between EI and the treatment response at the week 12 (t21 = -5.361, p = 6.37e-5). Moreover, among the clinical features, this rs-FC was associated with the early symptom improvement in the insomnia, somatic symptoms, and anxiety symptoms, and these early symptom improvements were associated with the treatment response. CONCLUSION: Rs-FC between the rAI and the left dlPFC played a crucial role in the early antidepressant effect, which linked the treatment response. The early treatment effect relating to rAI may represent an early symptom improvement in self-perceptual anxiety, somatic symptoms and insomnia.

2.
Small ; : e1904372, 2019 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-31609079

RESUMO

Minimization of defects and ion migration in organic-inorganic lead halide perovskite films is desirable for obtaining photovoltaic devices with high power conversion efficiency (PCE) and long-term stability. However, achieving this target is still a challenge due to the lack of efficient multifunctional passivators. Herein, to address this issue, n-type goethite (FeOOH) quantum dots (QDs) are introduced into the perovskite light-absorption layer for achieving efficient and stable perovskite solar cells (PSCs). It is found that the iron, oxygen, and hydroxyl of FeOOH QDs can interact with iodine, lead, and methylamine, respectively. As a result, the crystallization kinetics process can be retarded, thereby resulting in high quality perovskite films with large grain size. Meanwhile, the trap states of perovskite can be effectively passivated via interaction with the under-coordinated metal (Pb) cations, halide (I) anions on the perovskite crystal surface. Consequently, the PSCs with FeOOH QDs achieve a high efficiency close to 20% with negligible hysteresis. Most strikingly, the long-term stability of PSCs is significantly enhanced. Furthermore, compared with the CH3 NH3 PbI3 -based device, a higher PCE of 21.0% is achieved for the device assembled with a Cs0.05 FA0.81 MA0.14 PbBr0.45 I2.55 perovskite layer.

3.
ACS Nano ; 2019 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-31651142

RESUMO

Tumor microenvironment responsive multimodal synergistic theranostic strategies can significantly improve the therapeutic efficacy while avoiding severe side effects. Inspired by the fact that special morphology could enhance photothermal conversion efficiency (PCE) and cellular delivery, we developed an acidic tumor microenvironment responsive shape-reversal metal-organic virus-inspired nanodrug for enhancing near-infrared (NIR)-II PCE, increasing cell adhesion, and activating tumor targeting. First, a NIR-I fluorescence probe (IR825), a chemo-drug (pemetrexed, PEM), and a rare-earth metal ion (Nd(III)) were chosen to synthesize a virus-like nanodrug via coordination-driven assembly. Then, the spike-like surface of the nanodrug was further camouflaged by an acidity-sensitive poly(ethylene glycol) "shell" to create virus-core and sphere-shell hierarchical nanoassemblies, which could efficiently prevent immune clearance and prolong systemic circulation. Interestingly, the acidic tumor microenvironment could trigger the shell detachment of nanoassemblies for shape reversal to produce a virus-like surface followed by re-exposure of PEM to synergistically amplify the cellular internalization while enhancing NIR-II PCE. By utilizing the shell-detached virus-like nanodrug core, the tumor microenvironment specific enhanced NIR-II photothermal chemotherapy can be realized under the precise guidance of fluorescence/photoacoustic imaging, thereby achieving complete tumor elimination without recurrence in a single treatment cycle. We envision that integrating the tumor microenvironment responsive ability with  "sphere-to-virus" shape reversal will provide a promising strategy for biomimetic targeted cancer therapy.

4.
Basic Res Cardiol ; 114(6): 41, 2019 09 09.
Artigo em Inglês | MEDLINE | ID: mdl-31502080

RESUMO

Carotid baroreceptor stimulation (CBS) has been shown to improve cardiac dysfunction and pathological structure remodelling. This study aimed to investigate the effects of CBS on the ventricular electrophysiological properties in canines with chronic heart failure (CHF). Thirty-eight beagles were randomized into control (CON), CHF, low-level CBS (LL-CBS), and moderate-level CBS (ML-CBS) groups. The CHF model was established with 6 weeks of rapid right ventricular pacing (RVP), and concomitant LL-CBS and ML-CBS were applied in the LL-CBS and ML-CBS groups, respectively. After 6 weeks of RVP, ventricular electrophysiological parameters and left stellate ganglion (LSG) neural activity and function were measured. Autonomic neural remodelling in the LSG and left ventricle (LV) and ionic remodelling in the LV were detected. Compared with the CHF group, both LL-CBS and ML-CBS decreased spatial dispersion of action potential duration (APD), suppressed APD alternans, reduced ventricular fibrillation (VF) inducibility, and inhibited enhanced LSG neural discharge and function. Only ML-CBS significantly inhibited ventricular repolarization prolongation and increased the VF threshold. Moreover, ML-CBS inhibited the increase in growth-associated protein-43 and tyrosine hydroxylase-positive nerve fibre densities in LV, increased acetylcholinesterase protein expression in LSG, and decreased nerve growth factor protein expression in LSG and LV. Chronic RVP resulted in a remarkable reduction in protein expression encoding both potassium and L-type calcium currents; these changes were partly amended by ML-CBS and LL-CBS. In conclusion, CBS suppresses VF in CHF canines, potentially by modulating autonomic nerve and ion channels. In addition, the effects of ML-CBS on ventricular electrophysiological properties, autonomic remodelling, and ionic remodelling were superior to those of LL-CBS.

5.
Hua Xi Kou Qiang Yi Xue Za Zhi ; 37(4): 450-452, 2019 Aug 01.
Artigo em Chinês | MEDLINE | ID: mdl-31512843

RESUMO

Negative pressure pulmonary edema is a rare complication of general anesthesia. This paper presents a case of acute negative pressure pulmonary edema that occurred during general anesthesia resuscitation. The patient is a young male that underwent bimaxillary surgery under general anesthesia. Laryngospasm spasm ensued after extubation. The treatment for laryngeal spasm retained the smoothness of the nasopharyngal airway, and the pulse oxygen saturation rapidly decreased after anesthesia resuscitation. Pink foam sputum was sucked out from the cavity due to respiratory shortness from mouth and nose. Highly concentrated oxygen was immediately given to assist ventilation and as a symptomatic support (diuretics, hormones), and the condition evidently improved. The diagnosis and treatment of this case suggest that when acute pulmonary edema occurs during general anesthesia resuscitation, negative pressure pulmonary edema should be highly suspected. The first line of treatment is to relieve respiratory tract obstruction. Supplying highly concentrated oxygen to assist positive pressure ventilation is an effective treatment to alleviate pulmonary edema.


Assuntos
Obstrução das Vias Respiratórias , Laringismo , Edema Pulmonar , Anestesia Geral , Humanos , Masculino , Resultado do Tratamento
6.
Int J Biol Macromol ; 139: 521-530, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31377297

RESUMO

Chitosan-1-(mercaptomethyl)-cyclopropane acetic acid (CS-MCA) copolymer was synthesized by amino linkage. The obtained copolymer was characterized by FTIR, 1H NMR, XRD, TGA and SEM. Porous and reticulate morphologies were found on the CS-MCA surface. The effects of pH on the rheological properties of CS-MCA were investigated. On the one hand, the apparent viscosity of CS-MCA indicated a shear-thinning behavior. The graft of MCA enhanced the moduli and the maximum elastic properties were observed at pH = 7.00. The addition of dithiothreitol reduced the viscosity and modulus of CS-MCA hydrogel, and the gelation time, temperature and frequency were obtained in dynamic oscillatory tests. The antibacterial effect of CS-MCA against E. coli was investigated for the inhibition zone and bacterial growth curve. These results showed that CS-MCA had better antibacterial ability than chitosan without modification. Therefore, the rheological behavior and functional activities can be applied for the hydrocolloid gels in food and pharmaceutical applications.

7.
Int Immunopharmacol ; 74: 105737, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31288152

RESUMO

Influenza A virus usually leads to economic loss to breeding farms and pose a serious threat to human health. Virus infecting tissues directly and influenza virus-induced excessive production of inflammatory factors play the key role in pathogenesis of the disease, but the mechanism is not well clarified. Here, the role of autophagy was investigated in H9N2 influenza virus-triggered inflammation. The results showed that autophagy was induced by H9N2 virus in A549 cells and in mice. Inhibiting autophagy by an autophagy inhibitor (3-methyladenine, 3-MA) or knockdown of Atg5(autophagy-related gene) by Atg5 siRNA significantly suppressed H9N2 virus replication, H9N2 virus-triggered inflammatory cytokines and chemokines, including IL-1ß, TNF-α, IL-8, and CCL5 in vitro and in vivo, and suppressed H9N2 virus-triggered acute lung injury as indicated as accumulative mortality of mice, inflammatory cellular infiltrate and interstitial edema, thickening of the alveolar walls in mice lung tissues, increased inflammatory cytokines and chemokines, increased W/D ratio in mice. Moreover, autophagy mediated inflammatory responses through Akt-mTOR, NF-κB and MAPKs signaling pathways. Our data showed that autophagy was essential in H9N2 influenza virus-triggered inflammatory responses, and autophagy could be target to treat influenza virus-caused lung inflammation.

8.
Clin Neurol Neurosurg ; 184: 105421, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31319236

RESUMO

McLeod syndrome (MLS) is a rare multisystem disorder and X-linked recessive inheritance disorder caused by mutations of the X-linked Kx blood group (XK) gene. The manifestations progress slowly and mainly appear in middle age, which make it difficult to distinguish MLS from other neuromuscular disorders. Here, we present a case of a 10-month-old Chinese boy who was taken to hospital for herpes of the extremities and oral cavity along with febrile seizures in June 2017. The laboratory test revealed persistent elevated levels of serum creatine phosphokinase and abnormal liver function. The results of the electrocardiogram showed sinus tachycardia, and magnetic resonance imaging of the brain showed enlarged bilateral ventricles and third ventricle. Genetic analysis by next-generation sequencing revealed a novel nonsense mutation c.89C > A (p. Ser30X) in exon 1 of XK. To the best of our knowledge, this is the first case report of infants with MLS confirmed by genetic analysis.

9.
Nat Hum Behav ; 3(9): 950-961, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31358974

RESUMO

Excessive alcohol consumption is one of the main causes of death and disability worldwide. Alcohol consumption is a heritable complex trait. Here we conducted a meta-analysis of genome-wide association studies of alcohol consumption (g d-1) from the UK Biobank, the Alcohol Genome-Wide Consortium and the Cohorts for Heart and Aging Research in Genomic Epidemiology Plus consortia, collecting data from 480,842 people of European descent to decipher the genetic architecture of alcohol intake. We identified 46 new common loci and investigated their potential functional importance using magnetic resonance imaging data and gene expression studies. We identify genetic pathways associated with alcohol consumption and suggest genetic mechanisms that are shared with neuropsychiatric disorders such as schizophrenia.

10.
Nat Commun ; 10(1): 2999, 2019 07 05.
Artigo em Inglês | MEDLINE | ID: mdl-31278262

RESUMO

The different genome-wide distributions of tri-methylation at H3K36 (H3K36me3) in various species suggest diverse mechanisms for H3K36me3 establishment during evolution. Here, we show that the transcription factor OsSUF4 recognizes a specific 7-bp DNA element, broadly distributes throughout the rice genome, and recruits the H3K36 methyltransferase SDG725 to target a set of genes including the key florigen genes RFT1 and Hd3a to promote flowering in rice. Biochemical and structural analyses indicate that several positive residues within the zinc finger domain are vital for OsSUF4 function in planta. Our results reveal a regulatory mechanism contributing to H3K36me3 distribution in plants.


Assuntos
Histona-Lisina N-Metiltransferase/metabolismo , Histonas/genética , Oryza/fisiologia , Proteínas de Plantas/metabolismo , Transativadores/metabolismo , Metilação de DNA/fisiologia , Flores/crescimento & desenvolvimento , Regulação da Expressão Gênica de Plantas/fisiologia , Regiões Promotoras Genéticas/genética , Ligação Proteica/genética
11.
Artigo em Inglês | MEDLINE | ID: mdl-31326579

RESUMO

OBJECTIVE: Cannabis consumption during adolescence has been reported as a risk factor for psychotic-like experiences (PLEs) and schizophrenia. However, brain developmental processes associated with cannabis-related PLEs are still poorly described. METHOD: A total of 706 adolescents from the general population who were recruited by the IMAGEN consortium had structural magnetic resonance imaging scans at both 14 and 19 years of age. We used deformation-based morphometry to map voxelwise brain changes between the two time points, using the pairwise algorithm in SPM12b. We used an a priori region-of-interest approach focusing on the hippocampus/parahippocampus to perform voxelwise linear regressions. Lifetime cannabis consumption was assessed using the European School Survey Project on Alcohol and other Drugs (ESPAD), and PLEs were assessed with the Comprehensive Assessment Psychotic-like experiences (CAPE) tool. We first tested whether hippocampus/parahippocampus development was associated with PLEs. Then we formulated and tested an a priori simple mediation model in which uncus development mediates the association between lifetime cannabis consumption and PLEs. RESULTS: We found that PLEs were associated with reduced expansion within a specific region of the right hippocampus/parahippocampus formation, the uncus (p = .002 at the cluster level, p = .018 at the peak level). The partial simple mediation model revealed a significant total effect from lifetime cannabis consumption to PLEs (b = 0.069, 95% CI = 0.04-0.1, p =2 × 10-16), as well as a small yet significant, indirect effect of right uncus development (0.004; 95% CI = 0.0004-0.01, p = .026). CONCLUSION: We show here that the uncus development is involved in the cerebral basis of PLEs in a population-based sample of healthy adolescents.

12.
Nanoscale ; 11(27): 12973-12982, 2019 Jul 21.
Artigo em Inglês | MEDLINE | ID: mdl-31263818

RESUMO

Nano-theranostic agents play important roles in the development of therapeutic methods for serious diseases. In this study, novel carbon dots (CDs) L-CD/C-CD were prepared from Gd(iii) salt/complexes, cationic polymers and citric acid in the hope that they would combine the abilities of gene delivery and multi-modal (MR/FL) imaging. The CDs inherited the properties of good water-solubility and positive charge from their precursor polymers. In vitro gene transfection results showed that the CDs have good transfection efficiency and anti-serum ability, especially for L-CD, which has 74 times higher transfection efficiency than PEI 25 kDa in the presence of 10% serum. The CDs exhibited bright fluorescence, which was stable for several days under various pH. Confocal laser scanning microscopy revealed that the CDs could image HeLa cells with blue or green fluorescence well, and realize the monitoring of the gene delivery process. Besides, the CDs showed favorable biocompatibility with excellent performance in longitudinal relaxivity rates (r1) of 11.4 mM-1 s-1 for L-CD and 57.6 mM-1 s-1 for C-CD, which were about 3-15 times higher than that of the clinical Gd reagent Gd-DTPA (3.75 mM-1 s-1). Furthermore, the CDs could perform in vivo tumor-specific MR-imaging more clearly than Gd-DTPA, which is attributed to their suitable particle size and their resulting greater accumulation at tumor site via the EPR effect. This study provides a promising strategy for constructing multi-functional CDs for tumor theranostics.

13.
Chem Commun (Camb) ; 55(61): 9011-9014, 2019 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-31290869

RESUMO

For effective hydrogen generation with remarkable durability, carbon nanotubes (CNTs) grown on Ni nanofibers and their post hydroxylation treatment engendered active Ni nanofiber catalysts an efficient decomposition of hydrous hydrazine with a turnover frequency (TOF) of 19.4 h-1 and an activation energy down to 51.05 KJ mol-1.

14.
J Biomed Nanotechnol ; 15(7): 1384-1400, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31196345

RESUMO

Branched macromolecules have been used as carriers for imaging probes and drug delivery systems because of their tunable molecular structures, as well as their regular nanoscale structures and dimensions. We designed and synthesized two tumor environment-responsive branched and gadolinium (Gd)-based glycopolymer conjugates and investigated their potency as highly effective and safe magnetic resonance imaging (MRI) contrast agents. These branched macromolecules were prepared by one-pot reversible addition fragmentation chain transfer (RAFT) polymerization and conjugating chemistry. A biodegradable GFLG oligopeptide was used to successfully link the branch-chains of the branched macromolecules, finally a conjugate of this branched macromolecule and DOTA-Gd (HB-pGAEMA-Gd) with a molecular weight (MW) of 124 kDa was produced. Meanwhile, to improve the ability of tumor-targeting, we conjugated a tumor-targeting cRGDyK cyclic peptide to the branched molecule to prepare a tumor-targeted branched macromoleculeDOTA-Gd conjugate (HB-pGAEMA-RGD-Gd) with a MW of 136 kDa. The prepared branched macromolecules had a nanoscale hydrodynamic particle size and could be degraded into lower MW fragments with the cathepsin B. The aqueous phase relaxation efficiency of HB-pGAEMA-RGD-Gd (12.3 mM-1s-1 and HB-pGAEMA-Gd (13.2 mM-1s-1 was four times higher than that of DTPA-Gd (2.9 mM-1s-1), a clinically used contrast agent. In comparison with DTPA-Gd, the branched macromolecular contrast agents significantly enhanced the MRI signal intensity at the tumor site in vivo, and the enhancement of MRI signal intensity was up to 6 times that of the DTPA-Gd owing to their high relaxation efficiencies and accumulation at the tumor site. In addition, in vitro and in vivo toxicity studies indicated that the degradable macromolecular contrast agents had no significant toxicity.


Assuntos
Neoplasias , Meios de Contraste , Gadolínio , Humanos , Substâncias Macromoleculares , Imagem por Ressonância Magnética
15.
J Biomed Nanotechnol ; 15(8): 1637-1653, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31219024

RESUMO

In this study, a linear glycopolymer-gadolinium conjugate (pGAEMA-DOTA-Gd) was prepared via the reversible addition fragmentation chain transfer (RAFT) polymerization. In addition, a crosslinked polymer-gadolinium conjugate (Crosslinked pGAEMA-DOTA-Gd) was prepared via a two-step RAFT polymerization approach, and its core was composed of the biodegradable oligopeptide GFLG. The features of the two glycopolymer-gadolinium conjugates as highly efficient and safe nanoscale contrast agents were discussed. These two glycopolymer-DOTA-Gd conjugates have aqueous dynamic particle sizes of 4.8 nm and 21.3 nm with neutral charges. Longitudinal relaxivity (r1) of these two glycopolymer-gadolinium conjugates were three to four times higher than that of clinical agent DTPA-Gd. Animal studies showed that pGAEMA-DOTA-Gd and Crosslinked pGAEMA-DOTA-Gd demonstrated higher signal intensity and the relative change in T1 value (ΔT1) in mouse liver and kidney, and prolonged blood circulation time compared to DTPA-Gd. Notably, the performance of the core-crosslinked glycopolymer conjugate was better than that of the linear polymer. Inductively coupled plasma mass spectrometry (ICP-MS) results showed that polymeric contrast agents, especially the crosslinked one, showed higher aggregation in mouse liver and kidney, and were mainly excreted through renal routes eventually. In vitro and in vivo toxicity studies in healthy mice including cytotoxicity, blood compatibility and systemic toxicity indicated the absence of significant toxicity. Therefore, the prepared glycopolymer-DOTA-Gd conjugates may have significant potential as highly efficient and safe nanoscale MRI contrast agents.


Assuntos
Imagem por Ressonância Magnética , Animais , Meios de Contraste , Gadolínio , Camundongos , Tamanho da Partícula , Polímeros
16.
Cell Biochem Funct ; 37(5): 385-394, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31140646

RESUMO

To explore the molecular mechanism of insulin on proliferation and differentiation of MC3T3-E1 cell under high glucose conditions. We first investigated the effect of different concentrations of insulin on the osteoblast cell proliferation and cell differentiation at various time points by MTT analysis, cell cycle analysis, and expression detection of differentiation genes. Then, we used 200 ng/mL of insulin to treat the osteoblast cell at different time points for identifying the common differentially expressed mRNAs among various time points by RNA sequencing. Thirdly, we performed the gene ontology (GO) and the Kyoto Encyclopaedia of Genes and Genomes (KEGG) analysis to explore the biological function of these common differentially expressed mRNAs. The results showed that insulin promoted the cell proliferation and differentiation of osteoblast cell. In RNA sequencing, a total of 31 common differentially expressed mRNAs were identified between different time points. Mt1, Tmem135, Avp, and Dlg2 were found to be associated with the new bone formation. In addition, three important signalling pathways, namely, lysosome, glutamatergic synapse, and chemokine signalling pathways, were found in the KEGG enrichment analysis. Our work demonstrated that insulin could promote the osteoblast cell proliferation and cell differentiation, which may play a key role in bone formation. SIGNIFICANCE OF THE STUDY: Our result showed that insulin could promote the proliferation and differentiation of osteoblast at both cellular and molecular levels, which may promote the new bone formation in the osteoblasts.


Assuntos
Diferenciação Celular/efeitos dos fármacos , Glucose/metabolismo , Insulina/farmacologia , Osteoblastos/citologia , Osteoblastos/efeitos dos fármacos , Células 3T3 , Animais , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Camundongos , Osteoblastos/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo
17.
Medicine (Baltimore) ; 98(20): e15626, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31096473

RESUMO

BACKGROUND: Because of the heterogeneity of hepatitis C virus (HCV) distribution of different genotypes, large-scale clinical trials on direct-acting antiviral (DAA) mainly included patients with genotype 1 and genotype 3 infection. Data on the efficacy of direct-acting antiviral agents in patients with chronic genotype 6 HCV infection are limited. METHODS: The PubMed, Embase, and the Cochrane Libraries were searched comprehensively. All published clinical trials assessing the efficacy of DAA therapy for patients with chronic genotype 6 HCV infection were included. Sustained virological response (SVR) and rapid virological response (RVR) were pooled. Additional meta-analyses were also performed to compare the efficacy of DAA therapy in HCV-6 versus HCV-1 or HCV-3 patients. RESULTS: Seventeen studies met the inclusion criteria and were included in our meta-analysis. The pooled SVR of all single arms was 95% [95% confidence interval (CI): 0.90-0.97]. The pooled RVR of all single arms was 97% (95% CI: 0.95-0.99). The SVR and RVR were both similar between HCV-6 and HCV-1 or HCV-3. Adverse events were common but rarely caused treatment interruption. CONCLUSION: Based on the available data, our results indicate that DAA treatment is effective and safe for patients with genotype 6 HCV infection, and the efficacy was similar compared to patients with genotype 1 HCV or genotype 3 HCV infection.


Assuntos
Antivirais/uso terapêutico , Hepatite C Crônica/tratamento farmacológico , Hepatite C Crônica/genética , Antivirais/administração & dosagem , Antivirais/efeitos adversos , Quimioterapia Combinada , Genótipo , Humanos , Resposta Viral Sustentada
18.
J Prosthet Dent ; 2019 May 09.
Artigo em Inglês | MEDLINE | ID: mdl-31079880

RESUMO

STATEMENT OF PROBLEM: Osseointegrated implants can be prone to occlusal overloading because of the absence of the periodontal ligament and limited tactile sensitivity. However, current scientific evidence of the occlusion variation of implant-supported fixed prostheses is lacking. PURPOSE: The purpose of this clinical study was to analyze changes in occlusal force distribution and occlusal contact in single posterior partial fixed implant-supported prostheses over time. MATERIAL AND METHODS: Partially edentulous patients who had received implant-supported single crowns in the posterior region between December 2012 and December 2013 were enrolled. The participants underwent occlusal examinations by using the T-Scan III system at 0.5, 3, 6, 12, 24, and 36 months after implant prosthesis delivery. The relative occlusal forces (ROFs) of implant prostheses, mesial adjacent teeth, and control natural teeth were recorded, and implant prosthesis occlusion time ratios were calculated. The paired t test was used to compare the implant prosthesis occlusion time ratios and ROFs of implant prostheses at 2 different times as a self-control. The differences in ROFs between implant prostheses and control teeth in the same participant at the same time were also analyzed by using a paired t test. The Pearson correlation coefficient was used to analyze the statistical correlation between implant prosthesis occlusal force and the implant prosthesis occlusion time ratio (α=.05). RESULTS: Thirty-seven posterior partial fixed implant-supported prostheses in 33 participants (18 women and 15 men aged 23.9 to 70 years) were followed up for 3 to 36 months (mean: 31.4 months). The ROFs of implant prostheses increased significantly (P<.05) from 2 weeks (7.46 ±4.21%) to 3 months (9.87 ±6.79%), whereas those of control natural teeth decreased significantly (P<.05) from 13.78 ±6.00% to 11.43 ±5.47%. The ROFs of implant prostheses continued to increase from 6 to 12 months and from 12 to 24 months, with significant differences (P<.05). However, they were statistically similar to those of control natural teeth at 6, 12, 24, and 36 months after restoration. Implant prosthesis occlusion time ratios also increased significantly between 2 weeks and 3 months and between 3 and 6 months (P<.05). No significant differences were found between the other time points (P>.05). CONCLUSIONS: The occlusal force and occlusal contact time of implant prostheses changed significantly with time.

19.
J Cell Biochem ; 120(9): 16244-16253, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31081255

RESUMO

BACKGROUND: Wound healing is a complex process in bone development. The aim of this study was to explore the molecular mechanism study of insulin in promoting wound healing. METHODS: Firstly, the acute human monocyte leukemia cell lines were induced to differentiate into macrophages. Secondly, the porphyromonas gingivalis was applied to mix with the differentiated macrophages. Thirdly, the effect of different concentrations of insulin (0 ng/mL, 5 ng/mL, 50 ng/mL, 100 ng/mL, 200 ng/mL, 500 ng/mL, and 1,000 ng/mL) on the phagocytosis of macrophages and production of reactive oxygen species was investigated. Depending on these experiments, the optimal insulin concentration was used to treat the macrophages at different time points (0 hours and 0.5 hours) to identify the differentially expressed mRNAs. Finally, functional analysis including gene ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) and protein-protein interaction (PPI) analysis was carried out to explore the biological function of these differentially expressed mRNAs. RESULTS: The test of phagocytosis function and production of reactive oxygen species showed that 200 ng/mL insulin treatment had a significant influence on antibacterial and production of reactive oxygen species. In RNA sequencing, a total of 415 (245 upregulated and 170 downregulated) differentially expressed mRNAs were identified between different time points. Two important signaling pathways including endocytosis and systemic lupus erythematosus were found in the KEGG enrichment analysis. In the PPI network, several hub proteins encoded by differentially expressed mRNA including ALB, HIP1R, RAB5A, HIST1H2BJ, HIST1H3G, and HIST1H2BO were identified. CONCLUSION: Our work demonstrated that several differentially expressed mRNAs, such as EGR1, RAB34, ALB, HIP1R, RAB5A, HIST1H2BJ, HIST1H3G, and HIST1H2BO and two important signaling pathways including endocytosis and systemic lupus erythematosus may play important roles in the bone wound healing.

20.
J Lipid Res ; 60(7): 1212-1224, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31126973

RESUMO

The sympathetic nervous system (SNS) regulates the functions of white adipose tissue (WAT) and brown adipose tissue (BAT) tightly. Carotid baroreceptor stimulation (CBS) efficiently inhibits SNS activation. We hypothesized that CBS would protect against obesity. We administered CBS to obese rats and measured sympathetic and AMP-activated protein kinase (AMPK)/ PPAR pathway responses as well as changes in perirenal WAT (PWAT), epididymal WAT (EWAT), and interscapular BAT (IBAT). CBS alleviated obesity-related metabolic changes, improving insulin resistance; reducing adipocyte hypertrophy, body weight, and adipose tissue weights; and decreasing norepinephrine but increasing acetylcholine in plasma, PWAT, EWAT, and IBAT. CBS also downregulated fatty acid translocase (CD36), fatty acid transport protein (FATP), phosphorylated and total hormone sensitive lipase, phosphorylated and total protein kinase A, and PPARγ in obese rats. Simultaneously, CBS upregulated phosphorylated adipose triglyceride lipase, phosphorylated and total AMPK, and PPARα in PWAT, EWAT, and IBAT. However, BAT and WAT responses differed; although many responses were more sensitive in IBAT, responses of CD36, FATP, and PPARγ were more sensitive in PWAT and EWAT. Overall, CBS decreased chronically activated SNS and ameliorated obesity-related metabolic disorders by regulating the AMPK/PPARα/γ pathway.

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