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1.
Artigo em Inglês | MEDLINE | ID: mdl-34693718

RESUMO

Dentin bonding based on a wet-bonding technique is the fundamental technique used daily in clinics for tooth-restoration fixation and clinical treatment of tooth-related diseases. Limited bonding durability led by insufficient adhesive infiltration in the demineralized dentin (DD) matrix is the biggest concern in contemporary adhesive dentistry. This study proposes that the highly hydrated noncollagenous protein (NCP)-formed interfacial microenvironment of the DD matrix is the root cause of this problem. Meanwhile, the endogenous phosphate groups of the NCPs are used as pseudonuclei to rapidly induce the formation of amorphous CaF2 nanoparticles in situ in the interfacial microenvironment. The DD matrix is thus reconstructed into a novel porous structure. It markedly facilitates the infiltration of dentin adhesives in the DD matrix and also endows the DD matrix with anticollapsing capability when water evaporates. Whether using a wet-bonding or air-drying mode, the bonding effectiveness is greatly promoted, with the 12 month bonding strength being about twice that of the corresponding control groups. This suggests that the nanoreinforced DD matrix eliminates the dependence of bonding effectiveness on the moisture status of the DD surface controlled only by experiences of dentists. Consequently, this bonding strategy not only greatly improves bonding durability but also overcomes the technical sensitivity of bonding operations of the total-etched bonding pattern. This exhibits the potential to promote dentin bonding and is of great significance to dentistry.

2.
Zhejiang Da Xue Xue Bao Yi Xue Ban ; 50(2): 179-186, 2021 04 25.
Artigo em Inglês | MEDLINE | ID: mdl-34137232

RESUMO

To investigate the effect of captopril on the dentin bonding durability of self-etch adhesive. Different concentrations of captopril ethanol solutions or captopril ethanol/water solutions were prepared to pretreat dentin as primer for the self-etch adhesives. The surface morphology of the dentin was observed with scanning electron microscopy (SEM). Based on the morphology analysis, the pretreatment condition was selected and two self-etch adhesives were employed to evaluate the improvement effect of the captopril pretreatment on the dentin bonding durability. : SEM showed that the pretreatment of captopril ethanol solutions and captopril ethanol/water solutions were able to remove the smear lay and partially expose collagen matrix. According to the SEM results, the pretreating condition of captopril ethanol/water solution with the pretreating time of was selected for further dentin bonding study. For Clearfil SEBOND system, the immediate bonding strength increased from to  (<0.05). After one-year aging, the bonding strength of the control group decreased markedly [(22.90±6.82) MPa, <0.05]; while the bonding strength of the captopril pretreated group kept steadily >0.05]. For Clearfil S BOND system, there was no significant difference in the immediate bonding strength between the experimental group [(4.07) MPa] and the control group[(4.11) MPa]. But after one-year aging, the bonding strength of the experimental group was higher than that of the control group <0.05]. : The pretreatment with captopril ethanol/water solution increases the dentin bonding strength of the self-etch adhesive systems and also improves the bonding durability.


Assuntos
Colagem Dentária , Adesivos Dentinários , Adesivos , Captopril , Dentina , Teste de Materiais , Microscopia Eletrônica de Varredura , Cimentos de Resina
3.
J Mater Chem B ; 8(25): 5472-5482, 2020 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-32463060

RESUMO

The construction of multiscale Ti surfaces of high osteogenic ability has always attracted significant attention in the fields of oral implantology and implantable biomaterials. However, to date, the absence of a solid understanding of the correlation between the multiscale surface structure and the biological properties is the main obstacle in the development of these multiscale implants. In this study, a series of novel multiscale Ti surfaces were prepared via a three-step subtractive method. Moreover, based on the grayscale analysis of SEM images, we developed multiscale surface topography analysis methods. The typical topography characteristics at each scale of a multiscale complex surface can be analyzed according to the corresponding magnified SEM images. Thus, the evolution rule of the surface topography from a simple surface to multiscale complex surfaces can be mathematically described. Based on this, the correlation between multiscale surface structures and the corresponding biological properties was established. For the multiscale surface of superior osteogenic capacity, strict inherent regularity was found among the structures at multiple scales (i.e., multiscale order), that is, there was a balance between the construction of the 3D collagen-like network nanostructure and the preservation of the typical topographical features of the pre-existing macro- and micro-structures of the classic micro-roughened surface. Moreover, it was further found that the multiscale-ordered hierarchical Ti surface structure could modulate ROS production and enhance macrophage M2 polarization to create an osteogenesis-favorable immuno-inflammatory microenvironment and synergistically exhibit superior biological capability. Consequently, an optimized collagen-like hierarchical surface with superior osteogenic abilities was achieved.


Assuntos
Materiais Biocompatíveis/farmacologia , Osteogênese/efeitos dos fármacos , Titânio/farmacologia , Células 3T3 , Animais , Materiais Biocompatíveis/química , Células Cultivadas , Técnicas Eletroquímicas , Camundongos , Tamanho da Partícula , Coelhos , Espécies Reativas de Oxigênio/metabolismo , Propriedades de Superfície , Titânio/química
4.
Biomater Sci ; 8(8): 2234-2244, 2020 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-32129324

RESUMO

In the present study, low molecular weight poly(propylene carbonate) (PPC, Mn = 3500), a biodegradable liquid polymer easily prepared from carbon dioxide (CO2), was modified into poly(propylene carbonate)diacrylate (PPC-DA) by acylation, and methoxy poly(ethylene glycol) (mPEG) was modified into methoxy poly(ethylene glycol) acrylate (mPEG-A). Using PPC-DA as the dispersant to dissolve hydrophobic doxorubicin (DOX) and the initiator, and with mPEG-A as the co-monomer and polymerisable surfactant, a biodegradable nanodrug with excellent biocompatibility was prepared by shear emulsification polymerization without surfactants or organic solvent residues. The nanodrug can be efficiently endocytosed by tumor cells and can rapidly release doxorubicin triggered by the acidic endosomal pH. As evidenced by experiments in tumor-bearing mice, such a nanodrug is stealthy during blood circulation, and targets tumor sites with high efficiency. Moreover, this nanodrug is more effective and less toxic than free doxorubicin. This study provides a green and versatile approach for preparing biodegradable nanodrugs via a simple and efficient process. Moreover, this study extends the applications of CO2 based polymers in the biomedical field, promoting the development of CO2 polymerization fixation.


Assuntos
Antineoplásicos/administração & dosagem , Dióxido de Carbono/química , Doxorrubicina/administração & dosagem , Nanopartículas/administração & dosagem , Polietilenoglicóis/administração & dosagem , Polipropilenos/administração & dosagem , Animais , Antineoplásicos/química , Sobrevivência Celular/efeitos dos fármacos , Doxorrubicina/química , Liberação Controlada de Fármacos , Emulsões , Endocitose , Células HeLa , Células Hep G2 , Humanos , Camundongos Endogâmicos BALB C , Camundongos Nus , Nanopartículas/química , Neoplasias/tratamento farmacológico , Neoplasias/patologia , Polietilenoglicóis/química , Polipropilenos/química , Solventes , Tensoativos , Carga Tumoral/efeitos dos fármacos
5.
Biomater Sci ; 8(5): 1394-1404, 2020 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-31922160

RESUMO

Injectable shear-thinning hydrogels can be prepared by the non-covalent interactions between hydrophilic polymers. Although electrostatic force is a typical non-covalent interaction, direct mixing of two oppositely charged polyelectrolytes usually leads to a complex coacervate rather than an injectable hydrogel. Herein, a facile approach is proposed to prepare a shear-thinning hydrogel by nanoengineering of polyelectrolytes. Nanosized cationic micelles with electroneutral shells were prepared by mixing methoxyl poly(ethylene glycol)-block-poly(ε-caprolactone) and poly(ε-caprolactone)-block-poly(hexamethylene guanidine) hydrochloride-block-poly(ε-caprolactone) in an aqueous solution. When sodium carboxymethyl cellulose was added into the micellar solution, the outer poly(ethylene glycol) shell of mixed micelles prevented the instant electrostatic interaction between poly(hexamethylene guanidine) hydrochloride segments and sodium carboxymethyl cellulose, resulting in a homogenous shear-thinning electrostatic (STES) hydrogel. Because of the cationic poly(hexamethylene guanidine) hydrochloride segments, this hydrogel exhibits strong antibacterial activity against both Gram-positive and Gram-negative bacteria. Furthermore, the poly(ε-caprolactone) core of the mixed micelles can efficiently encapsulate a hydrophobic drug. In this work, curcumin-loaded STES hydrogel prepared by this method was used as wound dressing material that can promote wound healing even in infected wounds by further reducing bacterial infection via releasing curcumin. The present study provides a facile strategy to prepare shear-thinning antibacterial hydrogels from polyelectrolytes, which has great potential in biomedical application.


Assuntos
Antibacterianos/farmacologia , Hidrogéis/farmacologia , Nanotecnologia , Polieletrólitos/farmacologia , Animais , Antibacterianos/síntese química , Antibacterianos/química , Linhagem Celular , Curcumina/química , Escherichia coli/efeitos dos fármacos , Voluntários Saudáveis , Humanos , Hidrogéis/síntese química , Hidrogéis/química , Masculino , Camundongos , Micelas , Osteoblastos/efeitos dos fármacos , Osteoblastos/patologia , Polieletrólitos/síntese química , Polieletrólitos/química , Ratos , Ratos Sprague-Dawley , Staphylococcus aureus/efeitos dos fármacos , Eletricidade Estática , Cicatrização/efeitos dos fármacos
6.
Chem Commun (Camb) ; 51(78): 14644-7, 2015 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-26290273

RESUMO

We reported a novel injectable doxorubicin-loaded hydrogel based on host-guest interaction and Schiff's base reaction. A supramolecular polymeric prodrug was prepared through the inclusion of adamantane-modified doxorubicin into the ß-cyclodextrin cavity on the polyaldehyde dextran chain, which was in situ crosslinked by carboxymethyl chitosan.


Assuntos
Hidrogéis , Polímeros/química , Pró-Fármacos/química , beta-Ciclodextrinas/química , Doxorrubicina/administração & dosagem , Portadores de Fármacos
7.
J Mater Chem B ; 3(5): 784-795, 2015 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-32262169

RESUMO

After nearly half a century of development under the guidance of the osseointegration theory, the major dilemmas for current implant dentistry are the implant associated infection and insufficient osseointegration. Moreover, biological aging of titanium (Ti) implants also brings great uncertainty to clinical results. In the present study, a novel nano-micro-hierarchical topography pattern is created by sandblasting and dual acid-etching on a Ti surface. The physico-chemical properties of the surfaces were characterized by scanning electron microscopy, contact angle measurement, X-ray photoelectron spectroscopy and X-ray diffraction. The effects of the hierarchical surfaces on osteoprogenitor cell growth and bacterial activities were separately evaluated. The optimized nano-micro-hierarchical Ti surface exhibits surprisingly topography-dependent antibacterial capacity via inhibiting bacterial adhesion of several species in the early stage and better osteogenesis ability than the microscaled surface. Aging studies demonstrate that, compared with the surface with a microscale structure, the nano-micro-hierarchical Ti surface has greater anti-aging ability manifested as being more capable to retain hydrophilicity and bioactivity during aging. Furthermore, the present study reveals that the biological aging of the Ti implant is attributed to two decisive factors during the aging period: the progressively thickened amorphous TiO2 layer by autoxidation and the unavoidable accumulation of hydrocarbons on the Ti implant surface.

8.
J Mater Chem B ; 3(15): 3024-3031, 2015 Apr 21.
Artigo em Inglês | MEDLINE | ID: mdl-32262502

RESUMO

Paclitaxel-loaded reduction-responsive core-crosslinked micelles were prepared in situ in aqueous media via"click" chemistry. An amphiphilic block copolymer with multiple pendant azide groups was first synthesized through the controlled ring-opening copolymerization of ε-caprolactone (CL) and 5,5-dibromomethyl trimethylene carbonate (DBTC) in the presence of methoxy poly(ethylene glycol) (mPEG) as a macroinitiator, followed by azidation. This amphiphilic block copolymer could self-assemble into micelles and paclitaxel (PTX) could be encapsulated into the micellar core to form PTX-loaded micelles, which were core-crosslinked in situ by propargyl dithiopropionate via"click" chemistry, to develop a reduction-responsive polymeric drug delivery system. The in vitro release studies revealed the minimized release of PTX under physiological conditions, whereas a burst release of PTX was observed in response to reductive conditions. The core-crosslinked micelles displayed efficient cell-uptake and reduction-responsive drug release due to the nanoscale diameter and splitting of disulfide bonds under a reductive environment, which was confirmed by confocal laser scanning microscopy using Nile red as a fluorescent probe. This kind of polymeric nano-carrier with excellent biocompatibility and quick reduction-response opens a new avenue to intracellular anticancer drug delivery.

9.
Sci Rep ; 4: 6172, 2014 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-25146099

RESUMO

The deficient osseointegration and implant-associated infections are pivotal issues for the long-term clinical success of endosteal Ti implants, while development of functional surfaces that can simultaneously overcome these problems remains highly challenging. This study aimed to fabricate sophisticated Ti implant surface with both osteogenic inducing activity and inherent antibacterial ability simply via tailoring surface topographical features. Micro/submciro/nano-scale structure was constructed on Ti by three cumulative subtractive methods, including sequentially conducted sandblasting as well as primary and secondary acid etching treatment. Topographical features of this hierarchical structure can be well tuned by the time of the secondary acid treatment. Ti substrate with mere micro/submicro-scale structure (MS0-Ti) served as a control to examine the influence of hierarchical structures on surface properties and biological activities. Surface analysis indicated that all hierarchically structured surfaces possessed exactly the same surface chemistry as that of MS0-Ti, and all of them showed super-amphiphilicity, high surface free energy, and high protein adsorption capability. Biological evaluations revealed surprisingly antibacterial ability and excellent osteogenic activity for samples with optimized hierarchical structure (MS30-Ti) when compared with MS0-Ti. Consequently, for the first time, a hierarchically structured Ti surface with topography-induced inherent antibacterial capability and excellent osteogenic activity was constructed.


Assuntos
Próteses e Implantes , Titânio/química , Antibacterianos , Técnicas de Cultura de Células , Osteoblastos/metabolismo , Próteses e Implantes/efeitos adversos , Propriedades de Superfície
10.
Arch Oral Biol ; 59(5): 524-9, 2014 May.
Artigo em Inglês | MEDLINE | ID: mdl-24632095

RESUMO

OBJECTIVE: To study the expression of ADAMTs-5 and TIMP-3 in temporomandibular joint osteoarthritis (TMJOA) model rats, to explore and confer the possible effects of ADAMTs-5 and TIMP-3 involved in the degradation of the early stage of OA. DESIGN: 32 SD rats were divided into four groups: 2-week control group (NC1), 2-week OA group (OA1), 4-week control group (NC2) and 4-week OA group (OA2). Each group had 8 rats. Injection of collagenase was used to build up the TMJOA model. HE staining was used to analyze the structural change of condyle cartilage. Western blot and RT-PCR were used to measure the expression of ADAMTs-5 and TIMP-3 in protein and mRNA levels respectively. RESULTS: HE analysis revealed that no significant changes were observed in NC1, NC2 and OA1 groups, while mild damages appeared in OA2 group. No significant differences were achieved in the expression of ADAMTs-5 in protein levels between NC1 and OA1, but the expression of ADAMTs-5 in 4-week group increased significantly compared to that in the NC2 group. On mRNA level, the expression of ADAMTs-5 in 2-week and 4-week OA groups increased significantly compared to that in the matched control group. Meanwhile, the expression of TIMP-3 decreased significantly, showing a completely different trend. CONCLUSIONS: The expression of ADAMTs-5 and TIMP-3 changed significantly in the early stage of TMJOA, which indicated that ADAMTs-5 and TIMP-3 may be play an important part in the initial stage of condylar cartilage degradation.


Assuntos
Proteínas ADAM/metabolismo , Cartilagem Articular/metabolismo , Osteoartrite/metabolismo , Articulação Temporomandibular/metabolismo , Inibidor Tecidual de Metaloproteinase-3/metabolismo , Proteína ADAMTS5 , Animais , Western Blotting , Modelos Animais de Doenças , Masculino , RNA Mensageiro/metabolismo , Ratos , Ratos Sprague-Dawley , Reação em Cadeia da Polimerase Via Transcriptase Reversa
11.
J Mater Chem B ; 2(43): 7612-7619, 2014 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-32261899

RESUMO

We report a facile strategy to synthesize pH-sensitive amphiphilic oligo(ethylene glycol) (OEG)-doxorubicin (DOX) alternative conjugates. Poly[oligo(ethylene glycol) malicate] (POEGM) with numerous pendent hydroxyl groups was first synthesized by the direct polycondensation of oligo(ethylene glycol) (OEG) with malic acid under mild conditions. Then, benzaldehyde groups were introduced into the POEGM backbone via esterification between the pendant hydroxyl groups and 4-formylbenzoic acid. DOX moieties were finally attached to the polymeric backbone via benzoic imine linkages to obtain the OEG-DOX conjugates. Because of the high molecular weight and alternate architecture, this type of amphiphilic OEG-DOX alternative conjugates can form stable micelles in aqueous solution with a high DOX loading content (38.2 wt%) and low critical micelle concentrations (0.021 mg mL-1). Due to the pH-sensitive benzoic imine linkages between the DOX moieties and polymeric backbone, DOX could be rapidly released from the micelles at pH 5.8, whereas only a minimal amount of DOX was released at pH 7.4 under the same conditions. The cytotoxicity assay indicates that the OEG-DOX conjugates show cytotoxic effects to MCF-7 tumor cells, while the corresponding polymer material POEGM-CHO exhibits a great biocompatibility for MCF-7 tumor cells. These pH-sensitive and high drug loading nano-carriers based on the OEG-DOX alternative conjugates provide a promising platform for targeted cancer therapy.

12.
Biomater Sci ; 2(10): 1367-1376, 2014 Oct 26.
Artigo em Inglês | MEDLINE | ID: mdl-32481913

RESUMO

A redox-responsive amphiphilic polymeric prodrug was synthesized in a facile way by polycondensation of oligo(ethylene glycol) with dicarboxylic acids including malic acid and 3,3'-dithiodipropionic acid , followed by esterification with ibuprofen, which was used as a model drug. Because of its amphiphilic nature and relatively high molecular weight, this polymeric prodrug can form stable micelles in aqueous media with a low critical micellar concentration (CMC). Free ibuprofen molecules can be steadily incorporated into the core of these micelles with a surprisingly high loading content (38.9 wt%), owing to hydrophobic interaction and π-π stacking with the ibuprofen moieties in the copolymer. The in vitro release results indicate that there was a relatively slow and sustained release of the conjugated ibuprofen moieties, while encapsulated ibuprofen molecules showed a rapid release. Furthermore, for both the conjugated ibuprofen and the encapsulated ibuprofen there was an accelerated release in the presence of 10 mM dl-dithiothreitol due to cleavage of the disulfide bonds, which lead to disassociation of the micelles. Notably, this prodrug was revealed to have excellent cell compatibilities via a cell counting kit-8 (CCK-8) assay. Confocal laser scanning microscope observations indicated that the micelles based on the polymeric prodrug can be taken up quickly by cells and present a redox-responsive drug release in cytoplasm. This kind of polymeric nanocarrier with a high drug loading content, low CMC, excellent biocompatibility and rapid response to a reductive environment may have tremendous scope in the area of controlled drug delivery.

13.
Biomater Sci ; 2(7): 980-989, 2014 Jul 03.
Artigo em Inglês | MEDLINE | ID: mdl-32481971

RESUMO

As we all know biochemical surface modification is promising for implantable biomedical device applications due to its ability to directly provide therapeutic molecular cues for tissue repair. However, presenting multiple molecular cues on implant surfaces in the proper way is challenging. In this study, a multi-component polyelectrolyte multilayer (PEM) coating composed of collagen type I, RGD peptide functionalized hyaluronic acid, and recombined human BMP-2 (rhBMP-2) was constructed on Ti via a layer by layer technique. Subsequently, this coating was crosslinked via disulfide bonds to form a surface gel coating with a semi-interpenetrating network. A disulfide-crosslinked RGD-containing biomimetic extracellular matrix coating that could serve as a reservoir for rhBMP-2 was thus obtained. The embedded rhBMP-2 displayed a sustained release profile and a strong resistance to the physiological environment. In vitro biological evaluation revealed that the resultant disulfide crosslinking bioactive coating could effectively modulate cellular behaviors of pre-osteoblasts such as adhesion, proliferation and differentiation. In vivo study further revealed that this coating could enhance the bone-to-implant integration characterized by the increased removal torque values.

14.
Nanomedicine (Lond) ; 8(5): 739-55, 2013 May.
Artigo em Inglês | MEDLINE | ID: mdl-23384699

RESUMO

AIM: This study aimed at constructing a novel disulfide-crosslinked collagen I/hyaluronic acid polyelectrolyte multilayer (PEM) coating incorporated with bFGF and arginine-glycine-aspartic acid on titanium via the layer-by-layer technique, and evaluating its biological effects. MATERIALS & METHODS: The surface topography and components, thickness, degradation behaviors and bFGF release profiles of the PEM coating were investigated. The effects of the PEM coating on osteoprogenitor cell growth and bone implant interfacial binding strength in the rabbit femur model were evaluated separately. RESULTS: The formation of disulfide bonds improved the stability of the PEM coating, resulting in a coating that can release bFGF in a slow and sustained manner. Biological evaluations revealed that the resultant PEM coating on titanium promoted various cell behaviors and enhanced the binding strength. CONCLUSION: The employed cotreatment regimen enabled bFGF and arginine-glycine-aspartic acid to have a synergistic effect on the cell responses, which, in turn, improved the osseointegration.


Assuntos
Substitutos Ósseos/química , Materiais Revestidos Biocompatíveis/química , Colágeno/química , Fator 2 de Crescimento de Fibroblastos/administração & dosagem , Ácido Hialurônico/química , Oligopeptídeos/administração & dosagem , Titânio/química , Fosfatase Alcalina/metabolismo , Animais , Substitutos Ósseos/metabolismo , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Materiais Revestidos Biocompatíveis/metabolismo , Colágeno/metabolismo , Dissulfetos/química , Dissulfetos/metabolismo , Fêmur/lesões , Fator 2 de Crescimento de Fibroblastos/farmacologia , Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos , Ácido Hialurônico/metabolismo , Masculino , Oligopeptídeos/farmacologia , Osseointegração/efeitos dos fármacos , Osteoblastos/citologia , Osteoblastos/efeitos dos fármacos , Osteoblastos/metabolismo , Osteocalcina/metabolismo , Osteogênese/efeitos dos fármacos , Próteses e Implantes , Propriedades de Superfície , Titânio/metabolismo
15.
Zhejiang Da Xue Xue Bao Yi Xue Ban ; 41(3): 239-44, 2012 May.
Artigo em Chinês | MEDLINE | ID: mdl-22723157

RESUMO

OBJECTIVE: To construct a multiple-scale organized implant surface with super-hydrophilicity. METHODS: The SiC paper polished titanium disc was sandblasted and treated with HF/HNO3 and HCl/H2SO4, then acid-etched with H2SO4/H2O2. The physicochemical properties of the surfaces were characterized by scanning electron microscope, static state contact angle and X-ray diffraction. MC3T3-E1 cells were used to evaluate the effects of the surface on the cell adhesion, proliferation and differentiation. RESULTS: The acid-etching process with a mixture of H2SO4/H2O2 superimposed the nano-scale structure on the micro-scale texture. The multiple-scale implant surface promoted its hydrophilicity and was more favorable to the responses of osteoprogenitor cells, characterized by increased DNA content, enhanced ALP activity and promoted OC production. CONCLUSION: A multiple-scale implant surface with super-hydrophilicity has been constructed in this study, which facilitates cell proliferation and adhesion.


Assuntos
Corrosão Dentária , Implantes Dentários , Titânio/química , Células 3T3 , Animais , Adesão Celular/efeitos dos fármacos , Diferenciação Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Interações Hidrofóbicas e Hidrofílicas , Camundongos , Propriedades de Superfície , Titânio/farmacologia
16.
Acta Biomater ; 8(2): 866-77, 2012 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-22040683

RESUMO

Surface modification of titanium (Ti) using biomolecules has attracted much attention recently. In this study, a new strategy has been employed to construct a stable and bioactive coating on Ti. To this end, a derivative of hyaluronic acid (HA), i.e. HA-GRGDSPC-(SH), was synthesized. The disulfide-crosslinked Arg-Gly-Asp (RGD)-containing collagen/hyaluronic acid polyelectrolyte membrane (PEM) coating was then fabricated on Ti through the alternate deposition of collagen and HA-GRGDSPC-(SH) with five assembly cycles and subsequent crosslinking via converting free sulphydryl groups into disulfide linkages (RGD-CHC-Ti group). The assembly processes for PEM coating and the physicochemical properties of the coating were carefully characterized. The stability of PEM coating in phosphate-buffered saline solution could be adjusted by the crosslinking degree, while its degradation behaviors in the presence of glutathione were glutathione concentration dependent. The adhesion and proliferation of MC3T3-E1 cells were significantly enhanced in the RGD-CHC-Ti group. Up-regulated bone specific genes, enhanced alkaline phosphatase activity and osteocalcin production, the increased areas of mineralization were also observed in the RGD-CHC-Ti group. These results indicate that the strategy employed herein may function as an effective way to construct stable, RGD-containing bioactive coatings on Ti.


Assuntos
Materiais Revestidos Biocompatíveis/farmacologia , Colágeno/farmacologia , Reagentes para Ligações Cruzadas/farmacologia , Eletrólitos/química , Teste de Materiais/métodos , Oligopeptídeos/farmacologia , Titânio/farmacologia , Fosfatase Alcalina/metabolismo , Sequência de Aminoácidos , Animais , Osso e Ossos/efeitos dos fármacos , Osso e Ossos/metabolismo , Calcificação Fisiológica/efeitos dos fármacos , Bovinos , Adesão Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Regulação da Expressão Gênica/efeitos dos fármacos , Ácido Hialurônico/síntese química , Ácido Hialurônico/química , Membranas Artificiais , Camundongos , Dados de Sequência Molecular , Especificidade de Órgãos/efeitos dos fármacos , Especificidade de Órgãos/genética , Osteoblastos/citologia , Osteoblastos/efeitos dos fármacos , Osteoblastos/enzimologia , Osteocalcina/metabolismo , Peptídeos/síntese química , Peptídeos/química , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Espectroscopia de Infravermelho com Transformada de Fourier , Propriedades de Superfície/efeitos dos fármacos
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