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Taiwan J Obstet Gynecol ; 60(2): 318-323, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33678334


OBJECTIVE: The aim of this work was to characterize the genetic abnormalities and prenatal diagnosis indications in one fetus with Cri-du-Chat syndrome with codependent 10q24.2-q26.3 duplication in prenatal screening. MATERIALS AND METHODS: A 31-year-old woman had a second trimester serum screening that indicated the fetus was at low risk. During this pregnancy, the woman underwent amniocentesis at 18+4 weeks' gestation because of adverse fertility history and nuchal fold thickening. Cytogenetic analysis and next-generation sequencing analysis were simultaneously performed to provide genetic analysis of fetal amniotic fluid. According to abnormal results, parental chromosome karyotype of peripheral blood was performed to analysis. RESULTS: CNV-seq detected a 14.00 Mb deletion at 5p15.33-p15.2 and a 34.06 Mb duplication at 10q24.2-q26.3 in the fetus. Cytogenetic analysis of the fetus revealed a karyotype of 46, XY, der(5) t(5;10) (p15.2;q26.3). The karyotype of pregnant women was 46,XX,t(5;10) (p15.2;q24.2). The pregnancy was subsequently terminated after sufficient informed consent. CONCLUSION: This is the first study that reports prenatal diagnosis of a Cri-du-Chat syndrome with concomitant 10 q24.2-q26.3 duplication. Adverse pregnancy history has to be as an important indicator for prenatal diagnosis, and the genetic factors of abnormal pregnancy should be identified before next pregnancy. Nuchal fold thickening is closely related to fetal abnormalities. Combined with ultrasonography, the use of CNV-seq will improve the diagnosis of submicroscopic chromosomal aberrations in fetuses with congenital anomalies.

Transtornos Cromossômicos/diagnóstico , Síndrome do Miado do Gato/diagnóstico , Trissomia/diagnóstico , Aborto Induzido , Adulto , Amniocentese , Transtornos Cromossômicos/embriologia , Transtornos Cromossômicos/genética , Cromossomos Humanos Par 10/genética , Síndrome do Miado do Gato/embriologia , Síndrome do Miado do Gato/genética , Análise Citogenética , Feminino , Humanos , Cariotipagem , Gravidez , Segundo Trimestre da Gravidez/sangue , Trissomia/genética , Ultrassonografia Pré-Natal
Bioresour Technol ; 175: 203-8, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-25459823


Microaerobic process has been proven to be an alternative pretreatment for the anaerobic digestion (AD) process in several studies. In this study, the effect of thermophilic microaerobic pretreatment (TMP) on the AD of corn straw was investigated. Results indicated that TMP process obviously improved the methane yield. The maximum methane yield was obtained at the oxygen loads of 5ml/g VSsubstrate, which was 16.24% higher than that of untreated group. The modified first order equation analysis showed the TMP process not only accelerated the hydrolysis rates but also reduced the lag-phase time of AD process. The structural characterization analysis showed cellulosic structures of corn straw were partly disrupted during TMP process. The crystallinity indexes were also decreased. In addition, large or destroyed pores and substantial structural disruption were observed after pretreatment. The results showed that TMP is an efficient pretreatment method for the AD of corn straw.

Biotecnologia/métodos , Metano/biossíntese , Zea mays/metabolismo , Anaerobiose , Biocombustíveis , Fermentação , Hidrólise , Consórcios Microbianos , Microscopia Eletrônica de Varredura , Oxigênio/metabolismo , Brotos de Planta/química , Brotos de Planta/metabolismo , Espectroscopia de Infravermelho com Transformada de Fourier , Difração de Raios X , Zea mays/química
Huan Jing Ke Xue ; 35(6): 2322-7, 2014 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-25158513


Methanogens play an important role in marine sediments, which are related to methane production and methane hydrate deposits. Methanogenic activity of marine gas field sediments was investigated using substrates that methanogens usually used as carbon sources. H2/CO2, methanol, methylamines and trimethylamines could support the growth and methane production of gas field sediments. 16S rRNA gene sequence analysis indicated that the predominant methanogens in the enrichment cultures were related to known cultured methanogens in the family Methanosarcinaceae of the order Methanosarcinales and the family Methanomicrobiales of the order Methanomicrobiales, with genera Methanococcoides, Methanogenium and Methanosarcina as major methanogens.

Euryarchaeota/classificação , Sedimentos Geológicos/microbiologia , Metano/biossíntese , Campos de Petróleo e Gás/microbiologia , Filogenia , RNA Ribossômico 16S/genética
Vector Borne Zoonotic Dis ; 13(6): 422-4, 2013 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-23473226


Toxoplasma gondii is an obligate intracellular protozoan parasite that can infect almost all warm-blooded animals and humans with a worldwide distribution. Bats are reservoirs for an increasing number of emerging zoonotic viruses, such as henipaviruses and severe acute respiratory syndrome coronavirus (SARS-CoV). However, little is known of T. gondii infection in bats. The objective of the present study was to determine the seroprevalence of T. gondii infection in bats in China. A total of 217 serum samples from 5 species of bats were collected between April, 2010, and August, 2011, from 4 provinces in China. Antibodies to T. gondii were determined using the modified agglutination test (MAT, 1:25 or higher). Antibodies to T. gondii were found in 26.5% (18/68) Megaderma lyra, 13.6% (12/88) Rousettus leschenaulti, 13.6% (3/22) Cynopterus sphinx, 20% (4/20) Vespertilio superaus, and 15.8% (3/19) Pipistrellus javanicus. Antibody titers ranged from 1:25 to 1:400, with titers of 1:200 detected in 4 of the 5 bat species. The present study suggests the likely occurrence of T. gondii infection in bats in China, and these bats are new putative hosts for T. gondii, which may pose a threat to human health.

Anticorpos Antiprotozoários/sangue , Quirópteros/parasitologia , Toxoplasma/imunologia , Toxoplasmose Animal/epidemiologia , Testes de Aglutinação/veterinária , Animais , China/epidemiologia , Reservatórios de Doenças , Feminino , Humanos , Masculino , Estudos Soroepidemiológicos , Toxoplasma/isolamento & purificação , Toxoplasmose Animal/parasitologia , Zoonoses
Vaccine ; 31(4): 698-703, 2013 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-23196208


Avian chlamydiosis is caused by Chlamydophila psittaci (Cp. psittaci) and major outer membrane protein (MOMP) of Cp. psittaci is an excellent vaccine candidate. In this study, the MOMP gene was expressed in rice callus by the Agrobacterium tumefaciens vector. The production of protein in transgenic rice seeds was confirmed and quantified by Western-blot and ELISA, the results demonstrating that the antigen was expressed stably. The transgenic rice seeds expressing the MOMP protein were administered by the oral route to BALB/c mice, which developed MOMP-specific serum IgG and fecal IgA antibodies and a splenocyte MOMP-specific proliferative response and significant levels of IFN-γ, IL-2, IL-4, IL-5 and TGF-ß production. Immunization with MOMP transgenic seeds induced partial protection (50%) against a lethal challenge with the highly virulent Cp. psittaci 6BC strain. Lung function after challenge was less affected compared non-MOMP immunized animals. The results demonstrate the feasibility of using transgenic rice seeds as an oral vaccine to generate protective immunity and reduce the lung lesions in mice against virulent Cp. psittaci 6BC strain. This finding has implications for further development of an oral vaccine against avian chlamydiosis.

Proteínas da Membrana Bacteriana Externa/metabolismo , Vacinas Bacterianas/imunologia , Chlamydophila psittaci/imunologia , Oryza/genética , Plantas Geneticamente Modificadas/genética , Psitacose/imunologia , Vacinas de Plantas Comestíveis/imunologia , Administração Oral , Animais , Anticorpos Antibacterianos/biossíntese , Proteínas da Membrana Bacteriana Externa/genética , Proteínas da Membrana Bacteriana Externa/imunologia , Vacinas Bacterianas/genética , Chlamydophila psittaci/genética , Imunização , Camundongos , Camundongos Endogâmicos BALB C , Oryza/metabolismo , Psitacose/prevenção & controle , Linfócitos T Citotóxicos/imunologia , Células Th1/imunologia , Vacinas de DNA/genética , Vacinas de DNA/imunologia , Vacinas de Plantas Comestíveis/genética
J Gen Virol ; 91(Pt 7): 1764-71, 2010 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-20181748


Seoul virus (SEOV) is one of the four hantaviruses known to cause haemorrhagic fever with renal syndrome. The medium genome segment encodes the Gn/Gc glycoproteins of SEOV, which form the major structural part of the virus envelope. Gc and/or Gn are the candidate antigens of hantavirus for induction of a highly immunogenic response for hantavirus vaccine. In this study, the immune response induced by a replication-competent recombinant canine adenovirus type 2 expressing the Gc protein of SEOV was evaluated in BALB/c mice. Sera from immunized mice contained neutralizing antibodies that could specifically recognize SEOV and neutralize its infectivity in vitro. Moreover, the recombinant virus induced complete protection against an intensive infectious challenge with approximately 1000 50 % infective doses for SEOV strain CC-2. Protective-level neutralizing antibodies were maintained for at least 20 weeks. This recombinant virus is therefore a potential alternative to the inactivated vaccine.

Proteínas E3 de Adenovirus/genética , Adenovirus Caninos/genética , Glicoproteínas/metabolismo , Vírus Seoul/genética , Vacinas Virais/imunologia , Animais , Anticorpos Antivirais/sangue , Linhagem Celular , Cães , Feminino , Deleção de Genes , Regulação Viral da Expressão Gênica , Glicoproteínas/genética , Camundongos , Camundongos Endogâmicos BALB C , Organismos Livres de Patógenos Específicos , Vacinas Sintéticas/imunologia