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1.
World J Microbiol Biotechnol ; 35(12): 180, 2019 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-31728755

RESUMO

Derived from RNA, 5'-ribonucleotides, especially Inosine-5'-monophosphate (IMP) and guanosine-5'-monophosphate (GMP), can enhance the umami taste of soy sauce. In this study, the RNA content of three different salt-tolerant yeasts was examined. The most valuable strain was subjected to atmospheric and room-temperature plasma (ARTP) mutagenesis, which improved its RNA content by 160.54%. Regular fermentation with RNA-enhanced strain failed to increase the amount of 5'-ribonucleotides in the soy sauce due to hydrolysis by phosphatase. A two-stage fermentation strategy was then carried out. Aroma compounds were mainly synthesized in the first stage, and RNA-enriched biomass was massively produced in the second stage followed by heat treatment to inactivate phosphatase. After the proposed strategy was applied, IMP and GMP in the soy sauce reached 68.54 and 89.37 mg/L, respectively. Moreover, the amounts of key aroma compounds and organic acids significantly increased. Results may provide new insights for improving the quality of soy sauce through microorganism breeding and fermentation control.

2.
Plant Mol Biol ; 2019 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-31722090

RESUMO

KEY MESSAGE: We developed a machine learning-based model to identify the hidden labels of m6A candidates from noisy m6A-seq data. Peak-calling approaches, such as MeRIP-seq or m6A-seq, are commonly used to map m6A modifications. However, these technologies can only map m6A sites with 100-200 nt resolution and cannot reveal the precise location or the number of modified residues in a transcript. To address this challenge, we developed a novel machine learning-based approach, named HLMethy, to assign labels to m6A candidates from noisy m6A-seq data. The multiple instance learning framework was adopted and two different training strategies were used to generate the classification model. To test the performance of our model, the m6A sites with single-base resolution were used and our model achieved comparable performance against existing instance-level predictors, which suggest that our model has the potential to improve the data quality of m6A-seq at reduced costs. What's more, our generic framework can be extended to other newly found modifications that are found by peak-calling approaches. The source code of HLMethy is available at https://github.com/liuze-nwafu/HLMethy.

3.
Inorg Chem ; 58(21): 14617-14625, 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31626549

RESUMO

Titanium-oxo clusters (TOCs) are attractive as a rapidly growing class of molecular materials due to their use as molecular models and precursors of nano-titanium-oxide. However, most TOCs can only be dissolved in nonaqueous solvents, which largely limits their potential applications in biological or environmental situations. Very few water-soluble TOCs were reported, which can be used directly in aqueous biomedical systems. However, until now, no research studies of such TOCs involved in biomedical fields have been documented. We report here a series of lanthanide-titanium-oxo clusters (LnTOCs) formulated as {H2@[Ln2Ti8(µ3-O)8(µ2-O)4(Ac)16]}3·24CH3CN·23H2O (Ln = Eu(III) 1, Tb(III) 2, and Yb(III) 3). The compounds are easily soluble in water and form a stable solution of the cluster aggregates (LnTOC-a). Therefore, nano-biocompatible TiO materals can be prepared from these LnTOCs just by dissolving them in water. The nanoscale aggregates in water solutions were characterized by SEI-MS, 1H NMR, XPS, IR, and EDS mapping. Using the EuTOC-a solution, excellent fluorescence sensor properties for biomolecule ascorbic acid were found. Furthermore, biocompatibility and fluorescent labeling properties of the EuTOC-a for HeLa cells were evaluated. The results indicated that water-soluble LnTOCs can be used to prepare biocompatible fluorescent Ln-Ti-O nanomaterials.

4.
Yi Chuan ; 41(10): 962-973, 2019 Oct 20.
Artigo em Chinês | MEDLINE | ID: mdl-31624058

RESUMO

With the improvement of growth traits and feed conversion rate, the abdominal fat rate of Chinese local breeds of broilers has been increasing. Excessive abdominal fat deposition not only reduces the slaughter rate and disease resistance of broiler chickens, but also produces waste due to the difficulty of fat treatment. In order to study the regulatory genes and pathways involved in abdominal fat deposition of broilers, we used high-fat diets to feed the Xinghua Chicken, which is a Chinese local breed. Two weeks after feeding, we found that the abdominal fat weight and rate of broilers in the high-fat diet group increased significantly, and the diameter and area of abdominal fat cells also increased significantly. Transcriptome sequencing of abdominal fat and livers showed that the differentially expressed genes in the abdominal fat were mainly enriched in the cell cycle, peroxisome proliferator- activated receptor (PPAR) and extracellular matrix (ECM) receptor signaling pathways. The differentially expressed genes in livers were also significantly enriched in the cell cycle pathway, as well as in the steroid biosynthesis and PPAR signaling pathway. By analyzing the common differentially expressed genes in abdominal fat and liver tissues, we found that these genes were also enriched in cell cycle. Finally, we used the chicken LMH (chicken hepatoma cell) cell line and chicken ICP (immortalized chicken preadipocytes) cell line to do the in vitro validation assays. We used high-fat and common medium to culture the cells. The results showed that after 48 hours, the high-fat medium could significantly promote cell cycle and increase the number of cells in S phase. Additionally, qRT-PCR results showed that the high-fat medium could significantly promote the expression of genes related to cell cycle. In conclusion, we found that high-fat diets activate the cell cycle progression of chicken hepatocytes and preadipocytes, promote cell proliferation, and then increase abdominal fat deposition.


Assuntos
Gordura Abdominal/fisiologia , Ciclo Celular , Galinhas , Transcriptoma , Animais , Linhagem Celular , Proliferação de Células , Perfilação da Expressão Gênica , Receptores Ativados por Proliferador de Peroxissomo , Receptores de Superfície Celular , Transdução de Sinais
5.
Asian J Androl ; 2019 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-31603140

RESUMO

The first-line treatment options for high-risk prostate cancer (PCa) are definitive external beam radiotherapy (EBRT) with or without androgen deprivation therapy (ADT) and radical prostatectomy (RP) with or without adjuvant therapies. However, few randomized trials have compared the survival outcomes of these two treatments. To systematically evaluate the survival outcomes of high-risk PCa patients treated with EBRT- or RP-based therapy, a comprehensive and up-to-date meta-analysis was performed. A systematic online search was conducted for randomized or observational studies that investigated biochemical relapse-free survival (bRFS), cancer-specific survival (CSS), and/or overall survival (OS), in relation to the use of RP or EBRT in patients with high-risk PCa. The summary hazard ratios (HRs) were estimated under the random effects models. We identified heterogeneity between studies using Q tests and measured it using I2 statistics. We evaluated publication bias using funnel plots and Egger's regression asymmetry tests. Seventeen studies (including one randomized controlled trial [RCT]) of low risk of bias were selected and up to 9504 patients were pooled. When comparing EBRT-based treatment with RP-based treatment, the pooled HRs for bRFS, CSS, and OS were 0.40 (95% confidence interval [CI]: 0.24-0.67), 1.36 (95% CI: 0.94-1.97), and 1.39 (95% CI: 1.18-1.62), respectively. Better OS for RP-based treatment and better bRFS for EBRT-based treatment have been identified, and there was no significant difference in CSS between the two treatments. RP-based treatment is recommended for high-risk PCa patients who value long-term survival, and EBRT-based treatment might be a promising alternative for elderly patients.

6.
Sci Total Environ ; 698: 134175, 2019 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-31518786

RESUMO

Although jarosite and visible light are important factors for the formation of acid mine drainage (AMD), the effects of combined jarosite and visible light on chalcopyrite biodissolution have not been explored until now. In order to fill this knowledge gap, the combined effects of jarosite and visible light on chalcopyrite dissolution mediated by Acidithiobacillus ferrooxidans were investigated. The results indicated that jarosite and visible light could significantly accelerate chalcopyrite biodissolution, thus releasing more copper ions, iron ions and producing more acid. This in turn suggests enhanced generation of AMD under these conditions. Biodissolution results, mineral surface morphology, mineralogical phase and elemental composition analyses revealed that the promotion of chalcopyrite dissolution by additional jarosite and visible light was mainly attributed to the acceleration of ferric iron/ferrous iron cycling and the inhibition of the formation of a passivation layer (jarosite and Sn2-/S0) on the surface of chalcopyrite. This study provides a better understanding of the effects of jarosite and visible light on chalcopyrite biodissolution. In the future, the influences of jarosite and visible light on chalcopyrite dissolution should be considered in AMD evaluation to ensure reliability.

7.
Int J Mol Med ; 44(5): 1888-1898, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31545417

RESUMO

Previously, a ubiquinol­cytochrome c reductase binding protein (UQCRB) homolog was identified in the house dust mite (HDM) species Dermatophagoides farinae (Der f) as a major allergen. In the present study, the immunodominant immunoglobulin E (IgE) epitope of the protein Der f 24 was investigated. Analysis of the homologous amino acid (aa) sequences in Der f and human UQCRB was performed. Four different recombinant Der f 24 and hybrid proteins formed by integrating Der f and human UQCRB sequences were expressed in Escherichia coli, purified using Ni­NTA resins, and IgE­binding activity was determined using IgE­western blotting and enzyme­linked immunosorbent assay (ELISA) experiments. IgE epitopes were further identified by IgE­dot blotting and IgE­ELISA with synthetic polypeptides and HDM­allergic sera. Three­dimensional (3D) structural modeling was used to analyze the position of the immunodominant IgE epitope. The amino acid sequence homology between Der f 24 and the human UQCRB protein was determined to be 39.34%. IgE­ELISA and western blot analysis showed that all of the Der f­human UQCRB hybrid proteins generated, except for the one lacking 59 residues of the N­terminal region of Der f 24, were bound by allergic serum IgE. A synthetic polypeptide consisting of 32 residues of the N­terminal reacted with IgEs from HDM­allergic sera and could be used to generate high titer specific IgG or specific IgE antibodies in immunized mice. The 32­aa N­terminal region of Der f 24 was localized to a structural protrusion, which may facilitate specific IgE­binding. These results indicate that the immunodominant IgE epitope of Der f 24 is located mainly in a 32­residue region of the N­terminus. These findings may inform the mechanisms of HDM allergy sensitization and allergy immunotherapy development.

8.
iScience ; 19: 244-254, 2019 Jul 23.
Artigo em Inglês | MEDLINE | ID: mdl-31382187

RESUMO

Even though the energy density of O3-type layer-structured metal oxide cathode can fully reach the requirement for large-scale energy storage systems, the cycling lifespan still cannot meet the demand for practical application once it is coupled with a non-sodium-metal anode in full-cell system. Transition metal dissolution into the electrolyte occurs along with continuous phase transformation and accelerates deterioration of the crystal structure, followed by migration and finally deposition on the anode to form a vicious circle. Surface engineering techniques are employed to modify the interface between active materials and the electrolyte by coating them with a thin layer of AlPO4 ion conductor. This stable thin layer can stabilize the surface crystal structure of the cathode material by avoiding element dissolution. Meanwhile, it can protect the anode from increased resistance by suppressing the dissolution-migration-deposition process. This technique is a promising method to improve the lifetime for the future commercialization.

9.
Soft Matter ; 15(33): 6725-6731, 2019 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-31389469

RESUMO

The adsorption of xylyl-substituted biquaternary ammonium salt Gemini surfactants with different spacer (C3 and C6) at polytetrafluoroethylene (PTFE) and polymethylmethacrylate (PMMA) surfaces has been investigated and the different adsorption parameters such as surface tension, contact angle, adhesional tension, solid-water interfacial tension and work of adhesion have been estimated. The results show that C3 and C6 have similar adsorption behaviors at PTFE and PMMA surfaces. C3 and C6 adsorb gradually at a PFTE-water interface via hydrophobic interactions and the adsorption amounts at the water-air interface are almost three times higher than those at the PTFE-water interface due to the steric hindrance effect. However, the contact angle keeps constant throughout the experimental concentration range because the decrease in surface tension just counterbalances the decrease in PFTE-water interfacial tension. On the other hand, C3 and C6 adsorb at the PMMA surface via polar interactions between xylyl and functional groups of PMMA before CMC. Similar to PTFE, the increase in PMMA-water interfacial tension compensates the decrease in surface tension and the contact angle also shows a stationary value before the CMC. A bi-layer structure of C3 and C6 will be formed at the PMMA-water interface via hydrophobic interaction and PMMA-water interfacial tension decreases consequently after the CMC, which results in the decrease in contact angle.

10.
Autoimmunity ; 52(4): 168-175, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31407595

RESUMO

This study investigated the influence of autophagy on the expression of Collagen type II and light chain 3 (LC-3) in the articular cartilage of osteoarthritis (OA) models. The expression of OA associated biomarkers namely Matrix metalloproteinase (MMP-13), NOD-, LRR- and pyrin domain-containing 3 (NLRP3) induced by destabilizing the medial meniscus operation (DMM) were also investigated. A total of 60 C57BL/6 mice were divided into (1) control; (2) DMM2; (3) DMM8; (4) rapamycin 2 weeks; and (5) rapamycin 8 weeks groups. Saffranin O-Fast green staining, histomorphometry and immunohistochemical methods were used for analysis. In the DMM group, the expression of the OA biomarkers MMP-13, NLRP3 significantly increased, whilst Collagen II and LC-3B levels were significantly lower than other experimental groups. We hypothesized that NLRP3 inhibits autophagy activation and delays disease progression.

11.
ChemSusChem ; 12(21): 4846-4853, 2019 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-31461571

RESUMO

Ni0.5 Ti2 (PO4 )3 /C NASICON-type phosphate is introduced as a new anode material for lithium-ion batteries (LIBs). Ni0.5 Ti2 (PO4 )3 /C was synthesized through the sol-gel route and delivered some remarkable electrochemical performances. Specifically, the Ni0.5 Ti2 (PO4 )3 /C electrode demonstrates a high rate capability performance and delivers high reversible capacities ranging from 130 mAh g-1 to about 111 mAh g-1 at current rates ranging from 0.1 C to 5 C in the voltage window of 1.85-3 V (vs. Li+ /Li). In the same voltage range, the material reaches an initial capacity of 105 mAh g-1 with a capacity retention of about 82 % after 1000 cycles at the high current rate of 10 C. The electrodes are also tested in the wider voltage range of 0.5-3 V (vs. Li+ /Li) and show good reversibility and rate capability performance. Moreover, the Ni0.5 Ti2 (PO4 )3 /C electrodes enable fast Li+ diffusion (in the order of 10-13  cm2 s-1 ) compared with other NASICON-type materials. As a result, a first discharge capacity of 480 mAh g-1 is reached.

12.
Int J Clin Pharmacol Ther ; 57(10): 520-530, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31262398

RESUMO

OBJECTIVE: To characterize the pharmacokinetics of trazodone hydrochloride (HCl) sustained-release tablets (TSR) and trazodone immediate-release formulation (TIR) and investigate the effects of food on the pharmacokinetics of the drug in healthy subjects. MATERIALS AND METHODS: Three open-label, randomized crossover trials of single-dose, multiple-dose, and food-drug interaction testing were conducted. A validated high-performance liquid chromatography-fluorescence method was used to measure the plasma concentration of trazodone, and a non-compartment model was used to obtain the pharmacokinetic parameters. AUC and Cmax dose proportionality were analyzed using a power model. RESULTS: TSR lacked dose proportionality over a dose range of 25 - 150 mg. In the food-drug interaction study, no significant changes in the pharmacokinetic parameters of the drug under the fed conditions were observed. Multiple dosage of TSR and TIR reached steady state after 7 days, with no accumulation phenomenon observed. The peak time and peak concentrations of TSR were significantly longer and lower, respectively, than those of TIR. CONCLUSION: TSR showed clear sustained-release characteristics, and food exhibited no significant effects on the pharmacokinetic parameters of trazodone. TSR and TIR reached steady state levels after 7 consecutive days of administration, with no accumulation phenomenon observed.


Assuntos
Preparações de Ação Retardada/farmacocinética , Trazodona/farmacocinética , Área Sob a Curva , Estudos Cross-Over , Interações Alimento-Droga , Humanos , Comprimidos
13.
ChemSusChem ; 12(17): 4046-4053, 2019 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-31257701

RESUMO

Metallic-state 2D SnS2 nanosheets with expanded lattice spacing and a defect-rich structure were synthesized by the intercalation of Ni into the van der Waals gap of SnS2 . The expanded lattice spacing efficiently enhanced the electrochemical performance of the SnS2 for sodium-ion batteries owing to the change electron state density and energy band structure. In operando synchrotron XRD and theoretical calculations were used to gain insight into the influence of foreign metal-ion doping and its location. The optimized architecture obtained by in situ uniform growth of nanosheets on carbon fibers significantly enhanced the electrochemical performance. The inherent advantages of this architecture are shorter paths for ion insertion and extraction, larger contact area for more sodium diffusion pathways, and superior electrolyte penetration. Benefiting from the Ni intercalated SnS2 bilayer, the internal adjustment of the electronic state and the enlarged interlayer spacing significantly enhanced the electron transport kinetics, which can be explained by the metallic-state properties. The integrated electrode exhibited an initial high reversible capacity of 795 mAh g-1 at 0.1 A g-1 , with a stable capacity retention of 666 mAh g-1 after 100 cycles. Good rate capability was also exhibited with specific capacities of 691, 564, 437 mAh g-1 at current densities of 200, 500, and 1000 mA g-1 , respectively.

14.
ACS Nano ; 13(8): 9247-9258, 2019 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-31334639

RESUMO

Phosphorus doping is an effective strategy to simultaneously improve the electronic conductivity and regulate the ionic diffusion kinetics of TiO2 being considered as anode materials for sodium ion batteries. However, efficient phosphorus doping at high concentration in well-crystallized TiO2 nanoparticles is still a big challenge. Herein, we propose a defect-assisted phosphorus doping strategy to selectively engineer the surface structure of TiO2 nanoparticles. The reduced TiO2-x shell layer that is rich in oxygen defects and Ti3+ species precisely triggered a high concentration of phosphorus doping (∼7.8 at. %), and consequently a TiO2@TiO2-x-P core@shell architecture was produced. Comprehensive characterizations and first-principle calculations proved that the surface-functionalized TiO2-x-P thin layer endowed the TiO2@TiO2-x-P with substantially enhanced electronic conductivity and accelerated Na ion transportation, resulting in great rate capability (167 mA h g-1 at 10 000 mA g-1) and stable cycling (99% after 5000 cycles at 10 A g-1). Combining in/ex situ X-ray diffraction with ex situ electron spin resonance clearly demonstrated the high reversibility and robust mechanical behavior of TiO2@TiO2-x-P upon long-term cycling. This work provides an interesting and effective strategy for precise heteroatoms doping to improve the electrochemical performance of nanoparticles.

15.
J Asian Nat Prod Res ; : 1-6, 2019 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-31357881

RESUMO

One new triterpene glycoside, asiaticoside I (1), along with seven known ones (2-8), were isolated from the aerial parts of Cimicifuga dahurica (Turcz.) Maxim. The structure of 1 was elucidated on the basis of extensive spectroscopic methods including 1D-NMR, 2D-NMR and MS data. The structures of known compounds were determined by comparison with the literature data. Compound 1 exhibited moderate cell growth inhibitory activities in vitro against HELF, non-small cell lung cancer A549, and pancreatic cancer PANC-1 cell lines, with IC50 values of 62.97, 43.19, and 60.40 µM, respectively.

16.
Nat Commun ; 10(1): 2441, 2019 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-31147551

RESUMO

The original version of this Article contained errors in Fig. 8. In panel a, the labels 'VEGF', 'Notch', 'p21', and 'P-ERK' were inadvertently omitted. This has been corrected in the PDF and HTML versions of the Article.

17.
PLoS One ; 14(6): e0217962, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31188850

RESUMO

To investigate the emotional conflict processing during the processing of emotional stimuli in individuals with different levels of social adjustment through developing an event-related potential (ERP) method, the study used positive words (happy), negative words (disgusted), positive faces and negative faces as experimental materials for a face-word Stroop emotional conflict task, which was completed by 34 participants. For the N2 component, there was a significant difference between the high and low social adjustment groups for the congruent condition; the low social adjustment group evoked more negative amplitude under the congruent condition. Under the incongruent condition, there was a marginally significant difference between the high and low social adjustment groups; the low social adjustment group evoked more negative amplitude under the incongruent condition. For the SP component, there were no significant differences for both the high and low social adjustment group between the congruent and incongruent conditions of emotional conflict. However, within the low social adjustment group, the incongruent evoked more positive amplitude. Our findings indicate that the difference in the emotional conflict process between individuals with high and low social adjustment mainly lies in the early processing stages of emotional information. That is, for both congruent and incongruent emotional stimuli, individuals with high social adjustment showed better emotional conflict monitoring, used less cognitive resources, and had a higher degree of automated processing than those with low social adjustment. During the later stages of emotional conflict processing, individuals with low social adjustment showed poorer conflict processing.

18.
Radiat Oncol ; 14(1): 104, 2019 Jun 13.
Artigo em Inglês | MEDLINE | ID: mdl-31196126

RESUMO

PURPOSE: Despite recent advances in multimodal treatments, the prognosis of patients with glioblastoma multiforme (GBM) remains poor. The aim of this study was to evaluate the efficacy of moderately hypofractionated simultaneous integrated boost intensity-modulated radiotherapy (SIB-IMRT) combined with temozolomide (TMZ) for the postoperative treatment of GBM. MATERIALS AND METHODS: From February 2012 to February 2018, 80 patients with newly diagnosed and histologically confirmed GBM in our institute were reviewed retrospectively. All patients underwent complete resection or partial resection surgery and then received hypofractionated SIB-IMRT with concomitant TMZ followed by adjuvant TMZ. A total dose of 64 Gy over 27 fractions was delivered to the gross tumor volume (GTV), clinical target volume 1 (CTV1) received 60 Gy over 27 fractions, and CTV2 received 54 Gy over 27 fractions. The progression-free survival (PFS) and overall survival (OS) rates and the toxicities were evaluated. Prognostic factors were analyzed using univariate and multivariate Cox models. RESULTS: The median follow-up was 16 months (range, 5~72 months). The median PFS was 15 months, and the 1-, 2-, and 3-year PFS rates were 56.0, 27.6, and 19.5%, respectively. The median OS was 21 months, and the 1-, 2-, 3-, and 5-year OS rates were 77.6, 41.6, 32.8, and 13.4%, respectively. The toxicities were mild and acceptable. Age, KPS scores and the total number of TMZ cycles were significant factors influencing patient survival. CONCLUSION: Moderately hypofractionated SIB-IMRT combined with TMZ is a feasible and safe treatment option with mild toxicity and good PFS and OS.

19.
Development ; 146(13)2019 Jul 04.
Artigo em Inglês | MEDLINE | ID: mdl-31201155

RESUMO

During embryogenesis, epithelial organization is the prerequisite for organogenesis, in particular, for establishing the tubular structure. Recent studies provided hints about epithelial formation in early heart development, which has not been systemically explored. Here, we revealed a gradient of HAND2 protein in cardiac progenitors in the anterior dorsal pericardial wall (aDPW) and adjacent transition zone (TZ) in the outflow tract (OFT). Deletion of Hand2 caused cell arrest and accumulation in the TZ, leading to defective morphogenesis. Although apicobasal cell polarity was unaffected, the key epithelial elements of adherens junction and cell-matrix adhesion were disrupted in the TZ of Hand2 mutant mice, indicating poorly formed epithelium. RNA-seq analysis revealed altered regulation of the contractile fiber and actin cytoskeleton, which affected cardiomyocyte differentiation. Furthermore, we have identified Stars as being transcriptionally controlled by HAND2. STARS facilitates actin polymerization that is essential for anchoring the adhesive molecules to create cell adhesion. Thus, we have uncovered a new function of HAND2 in mediating epithelial maintenance and integrity in OFT morphogenesis. In addition, this study also provides insights for understanding cardiac progenitor contribution to OFT development.

20.
Int J Mol Sci ; 20(12)2019 Jun 14.
Artigo em Inglês | MEDLINE | ID: mdl-31208008

RESUMO

Excessive abdominal fat deposition is an issue with general concern in broiler production, especially for Chinese native chicken breeds. A high-fat diet (HFD) can induce body weight gained and excessive fat deposition, and genes and pathways participate in fat metabolism and adipogenesis would be influenced by HFD. In order to reveal the main genes and pathways involved in chicken abdominal fat deposition, we used HFD and normal diet (ND) to feed a Chinese native chicken breed, respectively. Results showed that HFD can increase abdominal fat deposition and induce adipocyte hypertrophy. Additionally, we used RNA-sequencing to identify the differentially expressed genes (DEGs) between HFD and ND chickens in liver and abdominal fat. By analyzed these DEGs, we found that the many DEGs were enriched in fat metabolism related pathways, such as peroxisome proliferator-activated receptor (PPAR) signaling, fat digestion and absorption, extracellular matrix (ECM)-receptor interaction, and steroid hormone biosynthesis. Notably, the expression of insulin-like growth factor II mRNA binding protein 1 (IGF2BP1), which is a binding protein of IGF2 mRNA, was found to be induced in liver and abdominal fat by HFD. Ectopic expression of IGF2BP1 in chicken liver-related cell line Leghorn strain M chicken hepatoma (LMH) cell revealed that IGF2BP1 can regulate the expression of genes associated with fatty acid metabolism. In chicken preadipocytes (ICP cell line), we found that IGF2BP1 can promote adipocyte proliferation and differentiation, and the lipid droplet content would be increased by overexpression of IGF2BP1. Taken together, this study provides new insights into understanding the genes and pathways involved in abdominal fat deposition of Chinese native broiler, and IGF2BP1 is an important candidate gene for the study of fat metabolism and adipogenesis in chicken.

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