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1.
Artigo em Inglês | MEDLINE | ID: mdl-31943851

RESUMO

Mesenchymal stem cells (MSCs) have been used in clinical studies to treat neurological diseases and damage. However, implanted MSCs do not achieve their regenerative effects by differentiating into and replacing neural cells. Instead, MSC secretome components mediate the regenerative effects of MSCs. MSC-derived extracellular vesicles (EVs)/exosomes carry cargo responsible for rescuing brain damage. We previously showed that EP4 antagonist-induced MSC EVs/exosomes have enhanced regenerative potential to rescue hippocampal damage, compared with EVs/exosomes from untreated MSCs. Here we show that EP4 antagonist-induced MSC EVs/exosomes promote neurosphere formation in vitro and increase neurogenesis and neuritogenesis in damaged hippocampi; basal MSC EVs/exosomes do not contribute to these regenerative effects. 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP) levels in EP4 antagonist-induced MSC EVs/exosomes are 20-fold higher than CNP levels in basal MSC EVs/exosomes. Decreasing elevated exosomal CNP levels in EP4 antagonist-induced MSC EVs/exosomes reduced the efficacy of these EVs/exosomes in promoting ß3-tubulin polymerization and in converting toxic 2',3'-cAMP into neuroprotective adenosine. CNP-depleted EP4 antagonist-induced MSC EVs/exosomes lost the ability to promote neurogenesis and neuritogenesis in damaged hippocampi. Systemic administration of EV/exosomes from EP4 -antagonist derived MSC EVs/exosomes repaired cognition, learning, and memory deficiencies in mice caused by hippocampal damage. In contrast, CNP-depleted EP4 antagonist-induced MSC EVs/exosomes failed to repair this damage. Exosomal CNP contributes to the ability of EP4 antagonist-elicited MSC EVs/exosomes to promote neurogenesis and neuritogenesis in damaged hippocampi and recovery of cognition, memory, and learning. This experimental approach should be generally applicable to identifying the role of EV/exosomal components in eliciting a variety of biological responses.

3.
Infect Genet Evol ; : 104181, 2020 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-31918040

RESUMO

Avian influenza A viruses (AIVs) classify into 18 hemagglutinin (HA) and 11 neuraminidase (NA) subtypes. Even though H1N1 and H3N2 subtypes usually circulate among humans leading to infection, occasionally, H5, H6, H7, H9, and H10 that circulate in poultry also infect humans, and especially H5N1 and H7N9. Efficient virus replication is a critical factor that influences infection. Codon usage of a virus must coevolve with its host for efficient viral replication, therefore, we conduct a comprehensive analysis of codon usage bias in human-isolated AIVs to test their adaptation to host expression system. The relative synonymous codon usage (RSCU) pattern, and the codon adaptation index (CAI) are calculated for this purpose. We find that all human-isolated AIVs tend to eliminate GC and CpG compositions, which may prevent activation of the host innate immune system. Although codon usage differs between AIV subtypes, our data support the conclusion that natural selection has played a major role and mutation pressure a minor role in shaping codon usage bias in all AIVs. Our efforts discover that codon usage of genes encoding surface proteins of H5N1, and the polymerase genes of H7N9 has better fit to the human expression system. This may associate with their better replication and infection in human.

4.
Huan Jing Ke Xue ; 41(1): 293-303, 2020 Jan 08.
Artigo em Chinês | MEDLINE | ID: mdl-31854930

RESUMO

To enhance the adsorption capacity of sepiolite (Sep) on Cd2+ in solution, an acid-base Sep (ABsep) was obtained using the method of combined acid-base modification. The structural properties of Sep and ABsep were analyzed by adsorption-desorption isotherms of N2, scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM-EDS), transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FTIR), and X-ray powder diffraction (XRD). Static adsorption experiments were carried out to evaluate the effects of time, mass ratio of ABsep/Cd2+, temperature, adsorbent dose, pH, and co-existing ions on the adsorption of Cd2+ by ABsep. The results showed that the pore structure of the ABsep was more developed than that of Sep. In comparison to Sep, the specific surface area, average pore diameter, and pore volume of ABsep increased by 66.1%, 15.7%, and 34.8%, respectively, and the exchangeable ion contents also increased. The main components of the ABsep were SiO2 and Mg(OH)2. The adsorption process of Cd2+ by Sep and ABsep could be well fitted with a pseudo-second-order kinetic equation and Langmuir isotherm, and both were spontaneous endothermic reactions, which were mainly chemical adsorption along with physical adsorption. The best mass ratio of ABsep:Cd2+ was 3:1, and the maximum saturated adsorption capacity fitted by the Sips model of the ABsep on Cd2+ at 298 K was 142.43 mg·g-1, which was 3.55 times that of Sep. As the adsorbent dose increased, the adsorption amounts of Cd2+ first increased and then decreased, with the optimum dose being 0.3 g·L-1. The amount of Cd2+ adsorption by the ABsep increased with the initial pH of the solution, whereby the best pH was 7. We also found that the presence of K+, Na+, Mg2+, and Ca2+ at different concentrations could inhibit the adsorption of Cd2+, whereby the inhibition of Mg2+ was the highest.

5.
Org Lett ; 22(2): 648-652, 2020 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-31887060

RESUMO

A novel Brønsted acid-catalyzed reaction of alkynyl thioethers with o-hydroxybenzyl alcohols via an unexpected formal [4 + 2] annulation has been developed. This metal-free protocol leads to the facile and practical synthesis of valuable polysubstituted 2H-chromenes in mostly good to excellent yields under mild reaction conditions and features a wide substrate scope and excellent functional group tolerance.

6.
Int J Biochem Cell Biol ; 118: 105660, 2019 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-31786325

RESUMO

BACKGROUND AND PURPOSE: Idiopathic pulmonary fibrosis (IPF) is a life-threatening progressive disease characterized by aberrant fibroblast activation. This study aims to explore the role of the circ0044226 on fibroblast-to-myofibroblast transition (FMT). METHODS: Bleomycin and TGF-ß1 were respectively used to induce the IPF mice model and human lung fibroblasts to myofibroblast differentiation. The mRNA and protein levels were examined by qRT-PCR and western blot. Localization of α-SMA was evaluated by immunofluorescence staining. Cell viability and proliferation were evaluated by CCK8 and EDU test. Dual-luciferase reporter assay was used to analyze the interaction between miR-7 and circ0044226 or sp1. Fluorescence in situ hybridization (FISH) assay was used for the identification of sub-location of circ0044226 and miR-7 in cells. The IPF model mice received intratracheal injection of AAV-sh-NC and AAV-sh- circ0044226, and lung fibrosis was detected by HE staining, Masson staining and immunohistochemistry assay. RESULTS: The circ0044226 was upregulated while miR-7 was downregulated in IPF mice model and FMT-derived myofibroblasts. miR-7 was a target of circ0044226 and sp1 was a target of miR-7. circ0044226 was distributed mostly in the cytoplasm and functioned as a miR-7 sponge to positively regulate the expression of sp1. Intervention of circ0044226 could ameliorate FMT and suppress fibroblast viability and proliferation by functioning as an endogenous miR-7 sponge. CONCLUSION: Circ0044226 knockdown alleviates fibroblast proliferation and FMT by functioning as a competing endogenous RNA, which may represent a promising therapy for pulmonary fibrosis.

7.
Coron Artery Dis ; 2019 Dec 19.
Artigo em Inglês | MEDLINE | ID: mdl-31860558

RESUMO

OBJECTIVE: We aimed to detect the synergistic effect between alcohol drinking, smoking and obesity on incident cardiovascular disease (CVD) in a Chinese population- based cohort. METHODS: We performed this study based on a prospective cohort based on a Chinese population in Jiangsu, China. Logistic regression was employed to detect the interaction of smoking, drinking with obesity on susceptibility to CVD, and calculate the odds ratio (OR) of CVD and corresponding 95% confidence interval (CI). RESULTS: A total of 3598 subjects (1451 males and 2147 females) were enrolled, including 82 CVD patients (36 males and 46 females) who new developed CVD at the follow-up. We found a significant abdominal obesity-current smoking interaction on CVD risk. Compared to never-smokers with normal waist circumference, OR (95% CI) of CVD were 2.44 (1.56-3.81), 1.58 (0.93-2.69), and 5.37 (3.08-9.34) for smokers with normal waist circumference, abdominal obese nonsmokers and abdominal obese smokers, respectively. Synergy index for this interaction was 2.35 (1.05-4.50). We also found a significant abdominal obesity-alcohol drinking interaction on CVD. Compared to never-drinkers with normal waist circumference, OR (95% CI) of CVD were 1.57 (1.01-2.45), 1.84 (1.08-3.12), and 4.44 (2.55-7.72) for drinkers with normal waist circumference, abdominal obese non- drinkers and abdominal obese drinkers, respectively. Synergy index for this interaction was 2.44 (1.04-5.72). CONCLUSION: We found significant interactions between alcohol drinking and abdominal obesity, smoking and abdominal obesity on CVD risk, suggested that the effect of alcohol drinking or smoking on CVD susceptibility seems to be modified by abdominal obesity.

8.
Biotechnol Prog ; : e2953, 2019 Dec 17.
Artigo em Inglês | MEDLINE | ID: mdl-31846227

RESUMO

Triton X-100 has long been used either alone or in combination with solvent to inactivate enveloped viruses in biopharmaceutical manufacturing. However, European Chemicals Agency (ECHA) officially placed Triton X-100 on the Annex XIV authorization list in 2017 because 4-(1,1,3,3-tetramethylbutyl) phenol, a degradation product of Triton X-100, is of harmful endocrine disrupting activities. As a result, any use of Triton X-100 in the European Economic Area would require an ECHA issued authorization after the sunset date of January 4, 2021. In search of possible replacements for Triton X-100, we discovered that polysorbate 80 (PS80) in absence of any solvents was able to effectively inactive enveloped viruses such as xenotropic murine leukemia virus and pseudorabies virus with comparable efficacy as measured by log reduction factors. Interestingly, PS80 did not show any virucidal activities in phosphate buffered saline (PBS) while achieving robust virus inactivation in cell-free Chinese hamster ovary (CHO) bioreactor harvests. This intriguing observation led us to speculate that virus inactivation by PS80 involved components in the cell-free CHO bioreactor harvests that were absent in PBS. Specifically, we hypothesized that esterase and/or lipases in the cell-free bioreactor harvests hydrolyzed PS80 to yield oleic acid, a known potent virucidal agent, which in turn inactivated viruses. This theory was confirmed using purified recombinant lysosomal phospholipase A2 isomer (rLPLA2) in PBS. Subsequent characterization work has indicated that virus inactivation by PS80 is effective and robust within temperature and concentration ranges comparable to those of Triton X-100. Similar to Triton X-100, virus inactivation by PS80 is dually dependent on treatment time and temperature. Unlike Triton X-100, PS80 inactivation does not correlate with concentrations in a simple manner. Additionally, we have demonstrated that PS20 exhibits similar virus inactivation activities as PS80. Based on the findings described in the current work, we believe that PS80 is potentially a viable replacement for Triton X-100 and can be used in manufacturing processes for wide spectrum of biopharmaceuticals to achieve desirable virus clearance. Finally, the advantages and disadvantages of using PS80 for virus inactivation are discussed in the contexts of GMP manufacturing.

9.
Spectrochim Acta A Mol Biomol Spectrosc ; 229: 117956, 2019 Dec 14.
Artigo em Inglês | MEDLINE | ID: mdl-31865098

RESUMO

It is of great significance to detect the moisture in organic solvents before used in water-sensitive reactions. Herein, two Schiff base quinoline derivatives, 8-hydroxyquinoline-2-carboxaldehyde thiosemicarbazone (HQCT) and 8-hydroxyquinoline-2-carboxaldehyde (pyridine-2-carbonyl)-hydrazine (HQPH), were designed and synthesized by a simple one-step reaction, and used as fluorescent chemosensors for ultra-fast and sensitive detection of water content in strong polar organic solvents. Based on excited-state intramolecular proton transfer (ESIPT) process, HQCT and HQPH exhibited strong fluorescence emissions with large Stokes shifts in dimethyl sulfoxide (DMSO) and N, N-dimethylformamide (DMF) solvents compared to other various organic solvents, and their fluorescence quenching and fluorescent color changes were obviously observed with increasing water content. The experimental results revealed that the hydroxyl groups substituted at the 8-position of HQCT and HQPH played a key role in the fluorescence emission processes. Dynamic light scattering (DLS) and 1H NMR titration indicated that the sensing mechanism for the detection of water was based on inhibition of the ESIPT by H2O via forming hydrogen bonds. In the range of 0.0-1.8 wt%, the fluorescence intensity of chemosensors changed as a linear function of water content. The detection limits of water in DMSO by HQCT and HQPH were as low as 0.0220 wt% and 0.0274 wt%, respectively. Moreover, HQCT and HQPH are successfully applied for the detection of moisture content in real commercial organic solvents.

10.
World J Microbiol Biotechnol ; 35(12): 180, 2019 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-31728755

RESUMO

Derived from RNA, 5'-ribonucleotides, especially Inosine-5'-monophosphate (IMP) and guanosine-5'-monophosphate (GMP), can enhance the umami taste of soy sauce. In this study, the RNA content of three different salt-tolerant yeasts was examined. The most valuable strain was subjected to atmospheric and room-temperature plasma (ARTP) mutagenesis, which improved its RNA content by 160.54%. Regular fermentation with RNA-enhanced strain failed to increase the amount of 5'-ribonucleotides in the soy sauce due to hydrolysis by phosphatase. A two-stage fermentation strategy was then carried out. Aroma compounds were mainly synthesized in the first stage, and RNA-enriched biomass was massively produced in the second stage followed by heat treatment to inactivate phosphatase. After the proposed strategy was applied, IMP and GMP in the soy sauce reached 68.54 and 89.37 mg/L, respectively. Moreover, the amounts of key aroma compounds and organic acids significantly increased. Results may provide new insights for improving the quality of soy sauce through microorganism breeding and fermentation control.

11.
Mol Genet Genomic Med ; : e1065, 2019 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-31777199

RESUMO

BACKGROUND: X-linked adrenoleukodystrophy (ALD) is one of the most common peroxisomal disorders characterized by abnormal accumulation of very long-chain fatty acids (VLCFA) in plasma and tissues and caused by mutations within ABCD1. Clinically, ALD present with various phenotypes, ranging from asymptomatic type to rapidly progressive childhood cerebral form. However, no remarkable abnormality in cerebral white matter usually makes it difficult to distinguish adult ALD from hereditary spastic paraplegia (HSP). METHODS: We analyzed the features of seven Chinese ALD patients who had a primary phenotype of spastic paraplegia. Sequencing was performed in the probands and their familial members. Detailed clinical, VLCFAs test, hormone test, magnetic resonance imaging, and electromyogram are presented. RESULTS: We reported seven ALD patients from a Chinese cohort of 142 HSP patients. Genetic investigations revealed five known ABCD1 mutations (c.346G>C, c.521A>G, c.829G>T, c.1415_1416delAG, and c.1849C>T) and two novel mutations (c.454C>G, c.1452_1482del). Further auxiliary testing revealed that they had higher VLCFA and/or adrenal insufficiency. CONCLUSIONS: Our findings expand the mutation spectrum of ABCD1 and indicate that ALD represent a significant portion (4.9%, 7/142) of the spastic paraplegia entities. ALD should be considered in male patients with spastic paraplegia, even if there was no positive family history.

12.
Molecules ; 24(23)2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31757097

RESUMO

An effective approach for amino protection and construction of a seven-membered ring has been developed. The method uses imidazolium chloride to carry out the Michael addition reaction at low temperatures and perform amino deprotection or construction of a seven-membered ring at high temperatures.

13.
World J Stem Cells ; 11(10): 859-890, 2019 Oct 26.
Artigo em Inglês | MEDLINE | ID: mdl-31692888

RESUMO

BACKGROUND: Treatments utilizing stems cells often require stem cells to be exposed to inflammatory environments, but the effects of such environments are unknown. AIM: To examine the effects of inflammatory cytokines on the morphology and quantity of mesenchymal stem cell exosomes (MSCs-exo) as well as the differential expression of microRNAs (miRNAs) in the exosomes. METHODS: MSCs were isolated from human umbilical tissue by enzymatic digestion. Exosomes were then collected after a 48-h incubation period in a serum-free medium with one of the following the inflammatory cytokines: None (control), vascular cell adhesion molecule-1 (VCAM-1), tumor necrosis factor (TNF) α, and interleukin (IL) 6. The morphology and quantity of each group of MSC exosomes were observed and measured. The miRNAs in MSCs-exo were sequenced. We compared the sequenced data with the miRBase and other non-coding databases in order to detect differentially expressed miRNAs and explore their target genes and regulatory mechanisms. In vitro tube formation assays and Western blot were performed in endothelial cells which were used to assess the angiogenic potential of MSCs-exo after inflammatory cytokine stimulation. RESULTS: MSCs-exo were numerous, small, and regularly shaped in the VCAM-1 group. TNFα stimulated MSCs to secrete larger and irregular exosomes. IL6 led to a reduced quantity of MSCs-exo. Compared to the control group, the TNFα and IL6 groups had more downregulated differentially expressed miRNAs, particularly angiogenesis-related miRNAs. The angiogenic potential of MSCs-exo declined after IL6 stimulation. CONCLUSION: TNFα and IL6 may influence the expression of miRNAs that down-regulate the PI3K-AKT, MAPK, and VEGF signaling pathways; particularly, IL6 significantly down-regulates the PI3K-AKT signaling pathway. Overall, inflammatory cytokines may lead to changes in exosomal miRNAs that abnormally impact cellular components, molecular function, and biological processes.

14.
Bioorg Med Chem ; : 115190, 2019 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-31744779

RESUMO

A novel series of graveolinine derivatives were synthesized and evaluated as potential anti-Alzheimer agents. Compound 5f exhibited the best inhibitory activity for acetylcholinesterase (AChE) and had surprisingly potent inhibitory activity for butyrylcholinesterase (BuChE), with IC50 values of 0.72 µM and 0.16 µM, respectively. The results from Lineweaver-Burk plot and molecular modeling study indicated non-competitive inhibition of AChE by compound 5f. In addition, these derivatives showed potent self-induced ß-amyloid (Aß) aggregation inhibition. Moreover, 5f didn't show obvious toxicity against PC12 and HepG2 cells at 50 µM. Finally, in vivo studies confirmed that 5f significantly ameliorates the cognitive performances of scopolamine-treated ICR mice. Therefore, these graveolinine derivatives should be thoroughly and systematically studied for the treatment of Alzheimer's disease.

15.
ACS Omega ; 4(19): 18195-18202, 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31720520

RESUMO

Considering the sharp increase in energy demand, Si-based composites have shown promise as high-performance anodes for lithium-ion batteries during the last few years. However, a significant volume change of Si during repetitive cycles may cause technical and security problems that limit the particular application. Here, an optimized reduced graphene oxide/silicon (RGO/Si) composite with excellent stability has been fabricated via a facile templated self-assembly strategy. The active silicon nanoparticles were uniformly supported by graphene that can further form a three-dimensional network to buffer the volume change of Si and produce a stable solid-electrolyte interphase film due to the increased specific surface area and enhanced intermolecular interaction, resulting in an increase of electrical conductivity and structural stability. As the anode electrode material of lithium-ion batteries, the optimized 10RGO/Si-600 composite showed a reversible high capacity of 2317 mA h/g with an initial efficiency of 93.2% and a quite high capacity retention of 85% after 100 cycles at 0.1 A/g rate. Especially, it still displayed a specific capacity of 728 mA h/g after 100 cycles at a reasonably high current density of 2 A/g. This study has proposed the optimized method for developing advanced graphene/Si nanocomposites for enhanced cycling stability lithium-ion batteries.

16.
Plant Mol Biol ; 101(6): 575-584, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31722090

RESUMO

KEY MESSAGE: We developed a machine learning-based model to identify the hidden labels of m6A candidates from noisy m6A-seq data. Peak-calling approaches, such as MeRIP-seq or m6A-seq, are commonly used to map m6A modifications. However, these technologies can only map m6A sites with 100-200 nt resolution and cannot reveal the precise location or the number of modified residues in a transcript. To address this challenge, we developed a novel machine learning-based approach, named HLMethy, to assign labels to m6A candidates from noisy m6A-seq data. The multiple instance learning framework was adopted and two different training strategies were used to generate the classification model. To test the performance of our model, the m6A sites with single-base resolution were used and our model achieved comparable performance against existing instance-level predictors, which suggest that our model has the potential to improve the data quality of m6A-seq at reduced costs. What's more, our generic framework can be extended to other newly found modifications that are found by peak-calling approaches. The source code of HLMethy is available at https://github.com/liuze-nwafu/HLMethy.

17.
Yi Chuan ; 41(10): 962-973, 2019 Oct 20.
Artigo em Chinês | MEDLINE | ID: mdl-31624058

RESUMO

With the improvement of growth traits and feed conversion rate, the abdominal fat rate of Chinese local breeds of broilers has been increasing. Excessive abdominal fat deposition not only reduces the slaughter rate and disease resistance of broiler chickens, but also produces waste due to the difficulty of fat treatment. In order to study the regulatory genes and pathways involved in abdominal fat deposition of broilers, we used high-fat diets to feed the Xinghua Chicken, which is a Chinese local breed. Two weeks after feeding, we found that the abdominal fat weight and rate of broilers in the high-fat diet group increased significantly, and the diameter and area of abdominal fat cells also increased significantly. Transcriptome sequencing of abdominal fat and livers showed that the differentially expressed genes in the abdominal fat were mainly enriched in the cell cycle, peroxisome proliferator- activated receptor (PPAR) and extracellular matrix (ECM) receptor signaling pathways. The differentially expressed genes in livers were also significantly enriched in the cell cycle pathway, as well as in the steroid biosynthesis and PPAR signaling pathway. By analyzing the common differentially expressed genes in abdominal fat and liver tissues, we found that these genes were also enriched in cell cycle. Finally, we used the chicken LMH (chicken hepatoma cell) cell line and chicken ICP (immortalized chicken preadipocytes) cell line to do the in vitro validation assays. We used high-fat and common medium to culture the cells. The results showed that after 48 hours, the high-fat medium could significantly promote cell cycle and increase the number of cells in S phase. Additionally, qRT-PCR results showed that the high-fat medium could significantly promote the expression of genes related to cell cycle. In conclusion, we found that high-fat diets activate the cell cycle progression of chicken hepatocytes and preadipocytes, promote cell proliferation, and then increase abdominal fat deposition.


Assuntos
Gordura Abdominal/fisiologia , Ciclo Celular , Galinhas , Transcriptoma , Animais , Linhagem Celular , Proliferação de Células , Perfilação da Expressão Gênica , Receptores Ativados por Proliferador de Peroxissomo , Receptores de Superfície Celular , Transdução de Sinais
18.
Asian J Androl ; 2019 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-31603140

RESUMO

The first-line treatment options for high-risk prostate cancer (PCa) are definitive external beam radiotherapy (EBRT) with or without androgen deprivation therapy (ADT) and radical prostatectomy (RP) with or without adjuvant therapies. However, few randomized trials have compared the survival outcomes of these two treatments. To systematically evaluate the survival outcomes of high-risk PCa patients treated with EBRT- or RP-based therapy, a comprehensive and up-to-date meta-analysis was performed. A systematic online search was conducted for randomized or observational studies that investigated biochemical relapse-free survival (bRFS), cancer-specific survival (CSS), and/or overall survival (OS), in relation to the use of RP or EBRT in patients with high-risk PCa. The summary hazard ratios (HRs) were estimated under the random effects models. We identified heterogeneity between studies using Q tests and measured it using I2 statistics. We evaluated publication bias using funnel plots and Egger's regression asymmetry tests. Seventeen studies (including one randomized controlled trial [RCT]) of low risk of bias were selected and up to 9504 patients were pooled. When comparing EBRT-based treatment with RP-based treatment, the pooled HRs for bRFS, CSS, and OS were 0.40 (95% confidence interval [CI]: 0.24-0.67), 1.36 (95% CI: 0.94-1.97), and 1.39 (95% CI: 1.18-1.62), respectively. Better OS for RP-based treatment and better bRFS for EBRT-based treatment have been identified, and there was no significant difference in CSS between the two treatments. RP-based treatment is recommended for high-risk PCa patients who value long-term survival, and EBRT-based treatment might be a promising alternative for elderly patients.

19.
Glob Chall ; 3(9): 1900027, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31565398

RESUMO

Developing an air electrode with high efficiency and stable performance is essential to improve the energy conversion efficiency and lifetime of zinc-air battery. Herein, Ni3Pt alloy is deposited on 3D nickel foam by a pulsed laser deposition method, working as a stable binder-free air electrode for rechargeable zinc-air batteries. The polycrystalline Ni3Pt alloy possesses high oxygen-conversion catalytic activity, which is highly desirable for the charge and discharge process in zinc-air battery. Meanwhile, this sample technique constructs an integrated and stable electrode structure, which not only has a 3D architecture of high conductivity and porosity but also produces a uniform Ni3Pt strongly adhering to the substrate, favoring rapid gas and electrolyte diffusion throughout the whole energy conversion process. Employed as an air electrode in zinc-air batteries, it exhibits a small charge and discharge gap of below 0.62 V at 10 mA cm-2, with long cycle life of 478 cycles under 10 min per cycle. Furthermore, benefitting from the structural advantages, a flexible device exhibits similar electrochemical performance even under the bending state. The high performance resulting from this type of integrated electrode in this work paves the way of a promising technique to fabricate air electrodes for zinc-air batteries.

20.
Inorg Chem ; 58(21): 14617-14625, 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31626549

RESUMO

Titanium-oxo clusters (TOCs) are attractive as a rapidly growing class of molecular materials due to their use as molecular models and precursors of nano-titanium-oxide. However, most TOCs can only be dissolved in nonaqueous solvents, which largely limits their potential applications in biological or environmental situations. Very few water-soluble TOCs were reported, which can be used directly in aqueous biomedical systems. However, until now, no research studies of such TOCs involved in biomedical fields have been documented. We report here a series of lanthanide-titanium-oxo clusters (LnTOCs) formulated as {H2@[Ln2Ti8(µ3-O)8(µ2-O)4(Ac)16]}3·24CH3CN·23H2O (Ln = Eu(III) 1, Tb(III) 2, and Yb(III) 3). The compounds are easily soluble in water and form a stable solution of the cluster aggregates (LnTOC-a). Therefore, nano-biocompatible TiO materals can be prepared from these LnTOCs just by dissolving them in water. The nanoscale aggregates in water solutions were characterized by SEI-MS, 1H NMR, XPS, IR, and EDS mapping. Using the EuTOC-a solution, excellent fluorescence sensor properties for biomolecule ascorbic acid were found. Furthermore, biocompatibility and fluorescent labeling properties of the EuTOC-a for HeLa cells were evaluated. The results indicated that water-soluble LnTOCs can be used to prepare biocompatible fluorescent Ln-Ti-O nanomaterials.


Assuntos
Materiais Biocompatíveis/química , Elementos da Série dos Lantanídeos/química , Nanoestruturas/química , Oxigênio/química , Titânio/química , Água/química , Células HeLa , Humanos , Tamanho da Partícula , Solubilidade
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