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1.
Nanoscale ; 12(3): 1994-2001, 2020 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-31912077

RESUMO

As a single-elemental system, tellurium can exist stably in the form of layers with an intriguing multivalence character, which constructs a new member of the 2D family. However, the growth and electronic structure of tellurium films are still far from known at present. Here, combined with molecular beam epitaxy, scanning tunneling microscopy/spectroscopy measurements and density functional theory calculations, we report the geometric and electronic structures of tellurium grown on NbSe2 from sub-monolayer to few-layer films. At the sub-monolayer coverage, we obtain two types of adatom-induced ordered superstructures that are strongly coupled with NbSe2. With the increase in coverage, the few-layer tellurium films adopt the α-phase form, showing internal strain-induced ripple patterns in the few-layers and bulk-like in thick layers with distinct edge geometries. The band gap of α-tellurium films decreases with the increase in thickness, which is associated with notable in-gap states. These observations, corroborated with DFT calculations, emphasize the important role of the NbSe2 substrate in modulating the structural and electronic properties of tellurium films. Moreover, the interaction between tellurium adatoms and tellurium films leads to √2 × âˆš2 surface reconstruction prior to a new monolayer, conforming to our theoretical calculations. Our work clarifies the kinetic growth of tellurium films on NbSe2 and reveals the tunability of electronic properties via substrate modulation or surface decoration.

2.
Fish Shellfish Immunol ; 98: 109-111, 2020 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-31911289

RESUMO

Mass mortality of juvenile hybrid (Haliotis discus hannai â™€× H. fulgens ♂, DF) and adult H. discus hannai (DD) occurs in south China during the summer. This study showed that the juvenile DF and adult DD exhibited significantly lower survival rates than juvenile DD and adult DF under 72 h pathogenic bacteria (Vibrio harveyi) challenge at different temperatures (20 °C and 28 °C). Phenoloxidase (PO) and superoxide dismutase (SOD) activities were significantly higher in juvenile DD compared to juvenile DF, whereas that in adult abalone was the opposite. Juvenile DD and adult DF also exhibited advantages in terms of immune-related gene expression (TRAF, TLR, MIF, Lys, Spi, Cat, TNF, and SOD) compared to juvenile DF and adult DD. The data reveals immunocompetence differences in DD and DF at the juvenile and adult stages.

3.
Biophys J ; 118(3): 565-577, 2020 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-31928762

RESUMO

Recruitment of leukocytes from blood vessels to inflamed zones is guided by biochemical and mechanical stimuli, with the mechanisms only partially deciphered. Here, we studied the guidance by the flow of primary human effector T lymphocytes crawling on substrates coated with ligands of integrins lymphocyte function-associated antigen 1 (LFA-1) (αLß2) and very late antigen 4 (VLA-4) (α4ß1). We reveal that cells segregate in two populations of opposite orientation for combined adhesion and show that decisions of orientation rely on a bistable mechanism between LFA-1-mediated upstream and VLA-4-mediated downstream phenotypes. At the molecular level, bistability results from a differential front-rear polarization of both integrin affinities, combined with an inhibiting cross talk of LFA-1 toward VLA-4. At the cellular level, direction is determined by the passive, flow-mediated orientation of the nonadherent cell parts, the rear uropod for upstream migration, and the front lamellipod for downstream migration. This chain of logical events provides a comprehensive mechanism of guiding, from stimuli to cell orientation.

4.
Nanoscale ; 12(4): 2406-2414, 2020 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-31782467

RESUMO

Wireless optogenetics based on the upconversion technique has recently provided an effective and interference-free alternative for remote brain stimulation and inhibition in behaving animals, which is of great promise for neuroscience research. However, more versatile upconversion devices are yet to be implemented for neural tissues other than the brain. In this study, a flexible and fully implantable upconversion device was developed for epidural spinal cord stimulation. The upconversion device was fabricated via a straightforward, two-step, heat-pulling process using biocompatible thermoplastic polypropylene as a backbone, which is mixed with upconversion nanoparticles (UCNPs) to form a flexible optrode device that converts near-infrared (NIR) irradiation to visible light for the optogenetic manipulation of spinal cord tissues. In this system, the flexible upconversion device is fully implantable within the rigid spine structure, and shows excellent long-term biocompatibility even after a four-month experiment. In anesthetized mice, the UCNP device implanted at the L4 vertebra can be used to reliably evoke hindlimb muscular activity upon NIR triggering. In behaving mice, neural modulation by the same UCNP devices effectively inhibits the animals' movement as a result of remote spinal cord stimulation. We believe that the flexible upconversion device provides new possibilities for wireless neural modulation in spinal cord tissues, and will become a valuable supplement to the current tool sets of upconversion based wireless optogenetics.

5.
Artigo em Inglês | MEDLINE | ID: mdl-31789139

RESUMO

BACKGROUND: Hashimoto's thyroiditis (HT) is characterized by lymphocytic infiltration of the thyroid parenchyma, which ultimately leads to tissue destruction and loss of function. Caveolin-1 (Cav-1) is an essential structural constituent of lipid rafts in the plasma membrane of cells and is reported to be significantly reduced in thyrocytes from HT patients. However, the mechanism of Cav-1 involvement in HT pathogenesis is still largely unclear. METHODS: Cav-1 expression in thyroid tissues from HT patients and euthyroid nodular goiter tissues was detected by immunohistochemistry staining. Cav-1 knockdown and overexpression were constructed by lentiviral transfection in the human thyroid follicular epithelial cell (TFC) line of Nthy-ori 3-1. The mRNA expression levels of chemokines in TFCs were determined by quantitative real-time PCR (qPCR). Cav-1 and peroxisome proliferator-activated receptor gamma (PPARγ) levels were analysed by qPCR and Western blot analysis. The migration ability of peripheral blood mononuclear cells (PBMCs) was detected by the Transwell assay. RESULTS: In this study, Cav-1 and PPARγ expression was reduced in the thyroid tissues from HT patients. In vitro experiments showed that the expressions of chemokine (C-C motif) ligand 5 (CCL5) and migration of PBMCs were markedly increased, while the level of PPARγ was significantly decreased after the lentivirus-mediated knockdown of Cav-1 in Nthy-ori 3-1 cells. Interestingly, pioglitazone, a PPARγ agonist, not only upregulated PPARγ and Cav-1 proteins significantly, but also effectively reversed the Cav-1-knockdown-induced upregulation of CCL5 in Nthy-ori 3-1 cells and reduced the infiltration of lymphocytes. CONCLUSION: The inhibition of Cav-1 upregulated the CCL5 expression and downregulated the PPARγ expression in TFC while pioglitazone, a PPARγ agonist, reversed the detrimental consequence. This outcome might be a potential target for the treatment of lymphocyte infiltration into the thyroid gland and HT development.

6.
ACS Appl Mater Interfaces ; 11(45): 42298-42304, 2019 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-31642318

RESUMO

We report an atomic-scale controllable synthesis of the face-centered cubic Ru overlayers on Pd nanosheets (Pd@Ru NSs) by a solution-based epitaxial growth method. The thickness of Ru overlayers can be accurately tuned at an atomic level, which has been confirmed by atomic force microscopy and high-angle annular dark-field scanning transmission electron microscopy. After annealing in air, the Pd@Ru NSs were transformed to PdO@RuO2 NSs with rutile RuO2 epitaxially grown on the PdO. The oxygen evolution reaction (OER) activity and stability strongly depend on the atomic layers of RuO2 and ∼4 atomic layers of RuO2 (PdO@RuO2-4layers) exhibit superior stability and optimal activity for OER with only 257 mV of the overpotential to reach 10 mA cm-2. Density functional theory calculations well reproduce the thickness dependence of OER activity and reveal that O* binds more weakly on the PdO@RuO2-4layers that boosts the rate-determining step for formation of HOO*, assuring the best OER performance.

7.
Sci Rep ; 9(1): 14545, 2019 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-31601972

RESUMO

Seafood coloration is typically considered an indicator of quality and nutritional value by consumers. One such seafood is the Xishi abalone (Haliotis gigantea), which displays muscle color polymorphism wherein a small subset of individuals display orange coloration of muscles due to carotenoid enrichment. However, the metabolic basis for carotenoid accumulation has not been thoroughly investigated in marine mollusks. Here, GC-TOF-MS-based untargeted metabolite profiling was used to identify key pathways and metabolites involved in differential carotenoid accumulation in abalones with variable carotenoid contents. Cholesterol was the most statistically significant metabolite that differentiated abalones with orange muscles against those with common white muscles. This observation is likely due to the competitive interactions between cholesterol and carotenoids during cellular absorption. In addition, the accumulation of carotenoids was also related to fatty acid contents. Overall, this study indicates that metabolomics can reflect physiological changes in organisms and provides a useful framework for exploring the mechanisms underlying carotenoid accumulation in abalone types.

8.
Rev Sci Instrum ; 90(9): 093702, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31575269

RESUMO

We present a continuous flow cryostat scanning tunneling microscope (STM) which can be operated at temperatures between 4.9 K and 300 K. A variable temperature insert with 20 mm aperture is installed in the cryostat. A base temperature of 4.9 K at the STM head was achieved with helium exchange gas cooling. By using a novel design of zirconia structure, the size of the STM head was minimized to 9.2 mm in outer diameter. The high compactness and rigidity of the STM head make it less sensitive to vibrations, contributing to the high stability of the tip-sample junction. The drifting rates of the STM at 4.9 K in the X-Y plane and Z direction are 1.96 and 3.05 pm/min, respectively. The performance of the STM was demonstrated by atomically resolved imaging of graphite and NbSe2 without using external vibrational isolation. Furthermore, the dI/dV spectra of NbSe2 were resolved near its critical temperature, illustrating the formation process of the superconducting gap as a function of temperature. This STM is ultrahigh vacuum and strong magnetic field compatible, making it promising for direct application in the extreme conditions involving water-cooled magnets and hybrid magnets.

9.
ACS Omega ; 4(8): 13577-13584, 2019 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-31460487

RESUMO

Copper(I) oxide (Cu2O) nanoparticles (NPs) are selectively prepared in high yields under continuous flow in a vortex fluidic device (VFD), involving irradiation of a copper rod using a pulsed laser operating at 1064 nm and 600 mJ. The plasma plume generated inside a glass tube (20 mm O.D.), which is rapidly rotating (7.5 k rpm), reacts with the enclosed air in the microfluidic platform, with then high mass transfer of material into the dynamic thin film of water passing up the tube. The average size of the generated Cu2ONPs is 14 nm, and they are converted to copper(II) oxide (CuO) nanoparticles with an average diameter of 11 nm by heating the as-prepared solution of Cu2ONPs in air at 50 °C for 10 h.

10.
Cell Mol Biol Lett ; 24: 45, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31297132

RESUMO

Background: The study aimed to investigate the effect of oxidative stress on Prestin expression, and explore the transcription factors (TFs) that are involved in regulating the expression of Prestin in House Ear Institute-Organ of Corti 1 (HEI-OC1) cells upon oxidative stress. Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot were used to detect the expression level of Prestin. Reverse chromatin immunoprecipitation (reverse ChIP) assay was performed to identify proteins that could bind to the Prestin gene. Small interfering RNA (siRNA) and chromatin immunoprecipitation (ChIP) experiments were used to further verify the results. HEI-OC1 cells were incubated with four different concentrations of tert-butyl hydroperoxide (t-BHP) for 24 h or 48 h to construct the oxidative stress model. Results: Oxidative stress induced Prestin increase at the mRNA level but with a concomitant decrease at the protein level. TF activating enhancer binding protein-2δ (AP-2δ) screened by reverse ChIP assay was demonstrated to bind to transcriptional start site 1441 of the Prestin promoter region and negatively regulate the expression of Prestin by siRNA and ChIP experiments. Furthermore, AP-2δ was down-regulated under oxidative stress. Conclusions: In conclusion, oxidative stress inhibits the expression of Prestin protein, and the transcription mechanism is triggered to compensate for the loss of Prestin protein. AP-2δ is one of the important TFs that suppresses transcription of the Prestin gene, and AP-2δ suppression further boosted Prestin mRNA activation under oxidative stress.


Assuntos
Regulação da Expressão Gênica , Proteínas Motores Moleculares/genética , Órgão Espiral/metabolismo , Estresse Oxidativo , Fator de Transcrição AP-2/metabolismo , Animais , Linhagem Celular , Camundongos , Órgão Espiral/citologia
11.
J Nutr ; 149(11): 1904-1910, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31334766

RESUMO

BACKGROUND: Dysfunction of the endoplasmic reticulum (ER) results in apoptosis, inflammation, and enhanced proteolysis in the small intestine of humans and animals. l-Glutamine (Gln) is required for intestinal mucosal homeostasis in piglets. However, a functional role of the ER in the enterocytes of weanling piglets and its contribution to intestinal mucosal integrity remain largely unknown. OBJECTIVE: This study was conducted to test the hypothesis that preweaning administration of Gln alleviates the activation of unfolded protein response (UPR) in the small intestine of weanling piglets. METHODS: Eighteen sow-reared piglets aged 7 d from 3 litters (6 piglets/litter) were assigned randomly into 1 of 3 treatment groups. Piglets were reared by sows until age 24 d, or were reared by sows and orally administered either l-alanine [1.84 g · kg body weight (BW)-1 · d-1] or Gln (1.52 g · kg BW-1 · d-1) twice daily between 7 and 21 d of age, and then weaned to a corn- and soybean meal-based diet. The small-intestinal samples were collected at 24 d of age for analyses of abundance of proteins related to ER stress and apoptosis, concentrations of inflammatory cytokines, and mRNA abundance for genes implicated in protein degradation. RESULTS: Compared with age-matched suckling piglets, weaning stress increased apoptosis and decreased cell proliferation in the jejunum. The abundance of proteins related to ER stress [binding immunoglobulin protein, activating transcription factor 6α, phosphorylated (p)-inositol-requiring kinase 1α, and p-eukaryotic initiation factor 2α] was elevated by 200% to 320%, and that of apoptotic proteins (CCAAT/enhancer-binding protein homologous protein, p-Jun-N-terminal kinase, caspase-12, cleaved caspase-3, and Bcl-2-associated X) was augmented by 100% to 350% in the jejunum of weanling piglets. The protein abundance for IL-1ß, TNF-α, and IL-8 was increased by 100% to 230% in the jejunum of weanling piglets. These alterations in gene and protein expression were markedly abrogated by Gln supplementation. The mRNA concentration of F-Box protein 32 in the jejunum of weanling piglets was increased by 70%, compared with the control group, and was not affected by Gln supplementation. CONCLUSION: Our results indicate that preweaning administration of Gln to nursing piglets alleviates the weaning-activated UPR.

12.
ACS Appl Mater Interfaces ; 11(26): 23039-23049, 2019 Jul 03.
Artigo em Inglês | MEDLINE | ID: mdl-31252506

RESUMO

Introduction of targeted defects into microporous UiO-66s for manipulating their three-dimensional size and surface properties can endow them with adsorption and separation areas involving angiotensin-converting-enzyme-inhibitory (ACE-inhibitory) peptides. Three hydrophobic amino acids (AAs) (i.e., proline (Pro), phenylalanine (Phe), and tryptophan (Trp)) having different physical/chemical properties were applied to in situ tailor defects in UiO-66 through targeted incoordination of missing linkers or missing nodes. Characterization results revealed a uniform oval shape of the developed defects with lengths ranging from 1.8 to 3.1 nm, which was also highly consistent with our molecular simulation. Among these three defective UiO-66s, Phe and Trp imprinted UiO-66s significantly promoted the adsorption affinity of small ACE-inhibitory peptides (uptake: 1.25 mmol g-1 for DDFF and 1.37 mmol g-1 for DDWW) and ultrahigh selectivity for DDFF (249) or DDWW (279) from inactive KKKK solution based on a lock-and-key mechanism. As a result, the imprinted UiO-66 showed an enrichment capacity for ACE-inhibitory peptides about eight times higher than that of pristine UiO-66. Therefore, the amino acid imprinting strategy endorsed by its facile and discerning ability can be envisioned to be of great value for small functional peptide separation and oriented enrichment in biomedicines.


Assuntos
Adsorção/efeitos dos fármacos , Inibidores da Enzima Conversora de Angiotensina/química , Peptídeos/química , Peptidil Dipeptidase A/química , Aminoácidos/química , Humanos , Peptidil Dipeptidase A/genética , Fenilalanina/química , Prolina/química , Propriedades de Superfície
13.
Ultramicroscopy ; 205: 20-26, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31234099

RESUMO

We present a probe-type scanning tunnelling microscope (STM) with atomic resolution that is designed to be directly inserted and work in a harsh vibrational cryogen-free superconducting magnet system. When a commercial variable temperature insert (VTI) is installed in the magnet and the STM is housed in the VTI, a lowest temperature of 1.6 K can be achieved, at which the STM still operates well. We tested the STM in an 8 T superconducting magnet cooled with a pulse-tube cryocooler and obtained atomically resolved graphite and NbSe2 images as well as the scanning tunnelling spectrum (i.e., dI/dV spectrum) data of the latter near its critical temperature, which show the formation process of the superconducting gap as a function of temperature. The drifting rates of the STM at 1.6 K in the X-Y plane and Z direction are 1.15 and 1.71 pm/min, respectively. Noise analysis for the tunnelling current shows that the amplitudes of the dominant peaks (6.84 and 10.25 Hz) are as low as 1.5 pA.Hz-1/2 when we set the current to 0.5 nA and open the feedback loop. This is important as a cryogen-free magnet system has long been considered too harsh for any atomic resolution measurement.

14.
Inorg Chem ; 58(14): 9036-9042, 2019 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-31246443

RESUMO

An excitonic insulating (EI) state is a fantastic correlated electron phase in condensed matter physics, driven by screened electron-hole interaction. Ta2NiSe5 is an excitonic insulator with a critical temperature (TC) of 328 K. In the current study, temperature-dependent Raman spectroscopy is used to investigate the phonon vibrations in Ta2NiSe5. The following observations were made: (1) an abnormal blue shift around TC is observed, which originates from the monoclinic to orthorhombic structural phase transition; (2) the splitting of a mode and two new Raman modes at 147 and 235 cm-1 have been observed with the formation of an EI state. With the help of first-principles calculations and temperature-dependent X-ray diffraction (XRD) experiments, it is found that the TaSe6 octahedra are "frozen" and the NiSe4 tetrahedra are greatly distorted below TC. Thus, it seems that the distortion of NiSe4 tetrahedra plays an important role in the strong electron-phonon coupling (EPC) in Ta2NiSe5, while the strong EPC, coupled with electron-hole interaction, opens the energy gap to form the EI state in Ta2NiSe5.

15.
Mol Genet Genomic Med ; 7(8): e808, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31250571

RESUMO

BACKGROUND: Nonsyndromic hearing loss (NSHL) is the most common sensorineural disorder and one of the most common human defects. Autosomal recessive inheritance accounts for a huge percentage of familial cases. Next-generation sequencing (NGS) is a powerful molecular diagnostic strategy for NSHL. The combination of a microarray gene chip and NGS can better delineate the etiology and genetic cause of deafness in many cases. METHODS: One hundred and thirty-one unrelated students with NSHL who attend a special education school in Yunnan Province were recruited. Firstly, four common deafness-related genes (GJB2, GJB3, SLC26A4, and mtDNA 12S rRNA) were evaluated for mutations using a microarray kit. Furthermore, 227 known human deafness genes were sequenced to identify the responsible genetic variant of the proband in three Chinese families with autosomal recessive hearing loss. The mutational status of family members of the probands was validated by Sanger sequencing. RESULTS: Five novel variants were found in three families using NGS. In family 1, we identified compound heterozygosity at the MYO15A (OMIM, #600316), including an duplication variant c.3866dupC, p.His1290Alafs*25 and a 3-bp deletion (c.10251_10253del, p.Phe3420del), resulting in protein length changes and premature protein truncation, respectively. In family 2, two affected siblings from a consanguineous Chinese Dai family harbored an c.1274G>C, p.Arg425Pro missense variant in the OTOF (OMIM, #601071). In family 3, we identified compound heterozygosity for c.129_130del, p.His43Glnfs*28 and c.76_79del, p.Lys26* in the RDX gene (OMIM, #611022). CONCLUSION: Five novel variants were found in three families with NSHL. Our findings extend the mutational spectrum in deafness-related genes and will help physicians in better understanding the etiology of hearing loss.

16.
Mar Biotechnol (NY) ; 21(4): 441-447, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31119501

RESUMO

Pacific abalone, Haliotis discus hannai, is an economically important marine mollusk species and an important model animal for studies on ecological, fertilization and developmental biology. While embryonic injection and genome editing have been wildly used in gene function study and trait improvement in many species, they have not been developed in abalones. In this study, we reported an effective method to inject exogenous materials in H. discus hannai unfertilized eggs. The injected eggs could be fertilized at a ratio of 52.6% ± 5.9% and hatch at a ratio of 14.6% ± 1.6%. On the base of this, we further developed an efficient genome editing approach in this species with the transcription activator-like effector nuclease (TALEN) technique. Two TALEN pairs targeting the coding sequence of the abalone nodal gene were assembled and tested. While one of the TALEN pairs showed no detectable mutation efficacy, the other one generated mutations in 50% of the targeted loci. The mutation includes small insertions and deletions and base pair replacements like that reported in other species when the TALEN method was applied. Overall, this is the first study to demonstrate site-specific genome editing in abalone. This work can serve as a reference for future studies focusing on the functional genomics in mollusks.


Assuntos
Sequência de Bases , Gastrópodes/genética , Edição de Genes/métodos , Genoma , Mutagênese Sítio-Dirigida/métodos , Deleção de Sequência , Nucleases dos Efetores Semelhantes a Ativadores de Transcrição/genética , Animais , Organismos Aquáticos , Embrião não Mamífero , Feminino , Fertilização , Gastrópodes/crescimento & desenvolvimento , Gastrópodes/metabolismo , Loci Gênicos , Masculino , Microinjeções/instrumentação , Mutagênese Insercional , Óvulo/citologia , Óvulo/metabolismo , Espermatozoides/citologia , Espermatozoides/fisiologia , Nucleases dos Efetores Semelhantes a Ativadores de Transcrição/metabolismo
17.
PLoS One ; 14(5): e0216816, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31145727

RESUMO

The high heat and mass transfer, and controlled mechanoenergy, in angled vortex fluidics has been applied in chemical and material sciences and allied fields, but its utility in food processing remains largely unexplored. Herein we report three models of food processing incorporating such vortex fluidics, including enzymatic hydrolysis, raw milk pasteurization and encapsulation. The processing times of enzymatic hydrolysis was reduced from about 2-3 hours to 20 minutes, with the processing time of raw milk pasteurization reduced from 30 to 10 minutes, and an encapsulated particle size reduced approximately 10-fold, from micro meters to hundreds of nanometers. These findings highlight exciting possibilities, in exploiting the value of vortex fluidic mediated processing in the food industry.


Assuntos
Laticínios , Manipulação de Alimentos , Pasteurização , Animais
19.
J Thorac Dis ; 11(3): 805-810, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31019768

RESUMO

Background: To determine the risk factors of delayed awakening following aortic arch surgery under deep hypothermic circulatory arrest (DHCA) in combination with selective antegrade cerebral perfusion (SACP). Methods: We retrospectively analyzed the clinical data of all patients who underwent aortic arch surgery under DHCA + SACP between September 2015 and September 2017 in our hospital. Delayed awakening was defined as recovery of consciousness later than 24 hours after the surgery. Risk factors of delayed awakening were evaluated using multivariate logistic regression analysis. Results: A total of 168 subjects were included. In-hospital mortality of the overall sample was 19.05% (n=32). Delayed awakening occurred in 76 (45.23%) subjects. Subjects with delayed awakening had older age, hypertension, higher rate of emergency surgery and blood transfusion, and longer cardiopulmonary bypass (CPB) time and myocardial blocking time. Multivariate regression analysis showed emergency surgery (P=0.005) and CPB time >240 min (P<0.001) as risk factors for delayed awakening, even after adjusting potential confounders, including age, hypertension, aortic cross-clamp time and blood transfusion. Conclusions: In patients undergoing aortic arch surgery under DHCA + SACP, emergency surgery and CPB time >240 min are risk factors for delayed awakening.

20.
Nanomaterials (Basel) ; 9(4)2019 Apr 09.
Artigo em Inglês | MEDLINE | ID: mdl-30970558

RESUMO

The simple yet efficient and versatile fabrication of colloidal crystals was investigated based on the solidification-induced colloidal crystallization process with particle/water suspension as precursor. The resulting colloidal crystals were constituted by crystal grains with sizes ranging from several tens of micrometers to a few millimeters. Each of the grains had a close-hexagonal array of colloids, which endowed the bulk colloidal crystal powders with some specific optical properties. The freezing of water was shown as the major driving force to form colloidal crystal grains, which supersaturated the solution with nanoparticles and thus induced the formation and growth of colloidal crystal seeds. This process is intrinsically different from those conventional methods based on shearing force, surface tension, columbic interaction or magnetic interaction, revealing a new strategy to fabricate colloidal crystals in a convenient and efficient way.

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