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1.
J Phys Condens Matter ; 32(22): 225301, 2020 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-32031992

RESUMO

Using ab initio calculations within the density functional theory, we explored the possible structures and properties of porous AlN monolayer materials. Two kinds of porous AlN monolayers (H- and T-) are identified. The phonon dispersion spectra together with the ab initio molecular dynamics simulations demonstrate that these structures are stable. We further show that the H- and T-AlN porous monolayers have well-defined porous nanostructures and high specific surface areas of 2863 m2 g-1 and 2615 m2 g-1 respectively, which is comparable to graphene (2630 m2 g-1), and can be maintained stably at high temperatures (>1300 K). Furthermore, both porous monolayers exhibit semiconductor properties, with indirect band gaps of 2.89 eV and 2.86 eV respectively. In addition, the electronic structures of the porous monolayers can be modulated by strain. The band gap of porous T-AlN monolayer experiences an indirect-direct transition when biaxial strain is applied. A moderate -9% compression can trigger this gap transition. These results indicate that porous AlN monolayers may potentially be used in future optoelectronic and catalyst applications.

2.
J Exp Clin Cancer Res ; 39(1): 30, 2020 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-32028981

RESUMO

BACKGROUND: BRD7 is a tumor suppressor known to inhibit cell proliferation and cell cycle progression and initiate apoptosis in breast cancer. However, the function and underlying molecular events of BRD7 in tumor invasion and metastasis in breast cancer are not fully understood. METHODS: BRD7 expression was assessed in two stable cell lines MDA231 and MCF7 with BRD7 overexpression and one stable cell line MDA231 with BRD7 interference using qRT-PCR and western blotting. CCK8 assay was used to examine the proliferation ability of MDA231 and MCF7 cells. Scratch wound healing assay was used to evaluate cell migration in MDA231 and MCF7 cells. Both Matrigel and three-dimensional invasion assays were performed to investigate the cell invasion ability after BRD7 overexpression or silencing or YB1 restoration in MDA231 and MCF7 cells. The potential interacting proteins of BRD7 were screened using co-immunoprecipitation combined with mass spectrometry and verified by co-immunoprecipitation in HEK293T cells. Additionally, we confirmed the specific binding region between BRD7 and YB1 in HEK293T cells by constructing a series of deletion mutants of BRD7 and YB1 respectively. Finally, xenograft and metastatic mouse models using MDA231 cells were established to confirm the effect of BRD7 on tumor growth and metastasis. RESULTS: Here, the results of a series of assays in vitro indicated that BRD7 has the ability to inhibit the mobility, migration and invasion of breast cancer cells. In addition, YB1 was identified as a novel interacting protein of BRD7, and BRD7 was found to associate with the C-terminus of YB1 via its N-terminus. BRD7 decreases the expression of YB1 through negatively regulating YB1 phosphorylation at Ser102, thereby promoting its proteasomal degradation. Furthermore, gene set enrichment analysis revealed that epithelial-mesenchymal transition (EMT) is the common change occurring with altered expression of either BRD7 or YB1 and that BRD7 represses mesenchymal genes and activates epithelial genes. Moreover, restoring the expression of YB1 antagonized the inhibitory effect of BRD7 on tumorigenicity, EMT, invasiveness and metastasis through a series of in vitro and in vivo experiments. Additionally, BRD7 expression was negatively correlated with the level of YB1 in breast cancer patients. The combination of low BRD7 and high YB1 expression was significantly associated with poor prognosis, distant metastasis and advanced TNM stage. CONCLUSIONS: Collectively, these findings uncover that BRD7 blocks tumor growth, migration and metastasis by negatively regulating YB1-induced EMT, providing new insights into the mechanism by which BRD7 contributes to the progression and metastasis of breast cancer.

4.
Cell Death Discov ; 5: 121, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31372241

RESUMO

Hepatocellular carcinoma (HCC) is one of the most common malignant tumors and a leading cause of cancer-related deaths worldwide. Emerging studies have shown that circular RNAs (circRNAs) are differentially expressed in HCC and play an important role in HCC pathogenesis and metastasis. However, the mechanism of circRNA in the chemoresistance of HCC remains unclear. In this study, we aimed to investigate the role of circRNA in cisplatin resistance of HCC. We identified a novel circRNA circRNA_101505 that was decreased in cisplatin-resistant HCC tissues and cell lines, and associated with a poor survival outcome. Gain-of-function investigations showed that overexpression of circRNA_101505 suppressed cancer cell growth in vivo and in vitro, and enhanced cisplatin toxicity in HCC cells. Mechanistic studies found that circRNA_101505 could sensitize HCC cells to cisplatin by sponging miR-103, and thereby promoting oxidored-nitro domain-containing protein 1 (NOR1) expression. In conclusion, the significant inhibitory effects indicate circRNA_101505 to be a potential therapeutic target for HCC treatment. Our findings provide significant evidence to further elucidate the therapeutic use of circRNA in HCC.

5.
Cell Death Dis ; 10(4): 319, 2019 04 09.
Artigo em Inglês | MEDLINE | ID: mdl-30967527

RESUMO

Clinical outcomes of patients with multiple myeloma (MM) have almost doubled the overall survival over the last decade owing to the use of proteasome inhibitor such as bortezomib (BTZ). However, some patients with MM develop primary resistance to BTZ, whereas others develop resistance after treatment. In this study, we investigated relationships between BTZ resistance and dysfunction of long non-coding RNAs (lncRNAs) in patients with MM. Bone marrow samples were collected from patients with MM and healthy donors for lncRNA microarray and survival analyses. To investigate functions and underlying mechanisms of lncRNA-mediated BTZ resistance in MM, we performed CCK-8 assays, flow cytometry analyses, dual luciferase report gene assays, and RNA pulldown assays with samples from nude mice carrying tumor xenografts and in clinical samples. Differentially expressed lncRNA myocardial infarction associated transcripts (MIAT) were highly expressed in patients with MM compared with healthy controls, and were predictive of poor survival outcomes. Moreover, MIAT expression was significantly increased in BTZ-resistant patients with MM compared with newly diagnosed patients with MM, and was identified as a BTZ-inducible lncRNA. Specifically, BTZ upregulated MIAT expression through increased stat1 phosphorylation. Silencing of MIAT inhibited MM cell growth and sensitized MM cells to BTZ by negatively regulating miR-29b. Our data demonstrated the utility of MIAT as a tool for overcoming BTZ resistance in patients with MM.

6.
Front Pharmacol ; 10: 45, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30766487

RESUMO

Aim: To investigate the prognostic value of lncRNA NR_046683 in multiple myeloma (MM). Methods: High-throughput lncRNA array was combined with bioinformatics techniques to screen differentially expressed lncRNA in MM. qRT-PCR was adopted to determine the expression of target lncRNAs in MM patients and controls. Results: It was found for the first time that lncRNA NR_046683 is closely related to the prognosis of MM. It was also detected in tumor cell lines KM3, U266, especially in drug-resistant cell lines KM3/BTZ and MM1R. The NR_046683 expression differed significantly in patients of different MM subtypes and staging. Moreover, the overexpression of NR-046683 is closely related to ß2-microglobulin. We also found that the overexpression of NR-046683 correlates to chromosomal aberrations, such as del(13q14), gain 1q21, and t(4;14). Conclusion: lncRNA NR_046683 can serve as a novel biomarker for potential drug target and prognostic prediction in MM.

7.
Carbohydr Polym ; 208: 328-335, 2019 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-30658808

RESUMO

The excess emission of toxic gases in atmosphere and heavy metal ions in drinking water is still a serious threat to human health. In this paper, a lightweight and porous zeolitic imidazolate frameworks-8@cellulose nanofiber@cellulose foam (ZIF-8@CNF@cellulose foam) with excellent gas adsorption and heavy metal ions removal properties was prepared using a simple in situ green growth method. The nitrogen adsorption property of ZIF-8@CNF@cellulose foam was 30 times higher than pure cellulose foam. Furthermore, the adsorption testing demonstrated that the composite foam showed high adsorption capacity for fluorescent dyes (24.6 mg g-1 for rhodamine B), heavy metal ions (35.6 mg g-1 for Cr (VI)) and organic solvents (45.2 g g-1 for DMF). Additionally, the ZIF-8/cellulose-based foam with 40 wt.% CNF exhibited an excellent mechanical performance, reaching a compressive strength value of 1.30 MPa. Herein, this work provides a feasible method to prepare ZIF-8@CNF@cellulose foam composite materials, which could adsorb gas molecules and heavy metal ions and show a great potential in atmosphere and water treatment.

8.
Cancer Sci ; 110(3): 939-949, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30618169

RESUMO

Tumor suppressor p53 is a master regulator of apoptosis and plays key roles in cell cycle checkpoints. p53 responds to metabolic changes and alters metabolism through several mechanisms in cancer. Lactate dehydrogenase A (LDHA), a key enzyme in glycolysis, is highly expressed in a variety of tumors and catalyzes pyruvate to lactate. In the present study, we first analyzed the association and clinical significance of p53 and LDHA in breast cancer expressing wild-type p53 (wt-p53) and found that LDHA mRNA levels are negatively correlated with wt-p53 but not with mutation p53 mRNA levels, and low p53 and high LDHA expression are significantly associated with poor overall survival rates. Furthermore, p53 negatively regulates LDHA expression by directly binding its promoter region. Moreover, a series of LDHA gain-of-function and rescore experiments were carried out in breast cancer MCF7 cells expressing endogenous wt-p53, showing that ectopic expression of p53 decreases aerobic glycolysis, cell proliferation, migration, invasion and tumor formation of breast cancer cells and that restoration of the expression of LDHA in p53-overexpressing cells could abolish the suppressive effect of p53 on aerobic glycolysis and other malignant phenotypes. In conclusion, our findings showed that repression of LDHA induced by wt-p53 blocks tumor growth and invasion through downregulation of aerobic glycolysis in breast cancer, providing new insights into the mechanism by which p53 contributes to the development and progression of breast cancer.


Assuntos
Neoplasias da Mama/genética , Neoplasias da Mama/patologia , Glicólise/genética , L-Lactato Desidrogenase/genética , Proteína Supressora de Tumor p53/genética , Linhagem Celular Tumoral , Movimento Celular/genética , Proliferação de Células/genética , Progressão da Doença , Regulação para Baixo/genética , Feminino , Regulação Neoplásica da Expressão Gênica/genética , Humanos , Células MCF-7 , Invasividade Neoplásica/genética , Invasividade Neoplásica/patologia , RNA Mensageiro/genética , Taxa de Sobrevida
9.
Talanta ; 196: 493-497, 2019 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-30683396

RESUMO

Acetate ion was widely used in pH buffer to control pH environment. Here we firstly found that acetate ion had mimic peroxidase activity. Acetate ions are capable of catalyzing the decomposition of hydrogen peroxide and play a similar role to that of horseradish peroxidase (HRP). Acetate catalyzes the oxidation of tetramethylbenzidine (TMB) by H2O2, which is the product of the reaction of glucose and glucose oxidase. A colorimetric sensor for H2O2 and glucose was developed using acetate ions. The linear regression equation for H2O2 was A = 0.0029 C + 0.0530 (C (µmolL-1), R = 0.9978), and the detection limit was 3.0 µmolL-1, whereas that for glucose was A = 0.0021 C + 0.0709 (C (µmol L-1), R = 0.9977), and the detection limit was 4.0 µmol L-1. Moreover, the proposed method was successfully applied for the detection of H2O2 in human urine and glucose in human serum; thus, the proposed method could be used for the diagnosis of illness or disease. A single-enzyme-based glucose test paper was firstly prepared and tested for semi-quantitative analysis of glucose.


Assuntos
Acetatos/química , Glucose/análise , Peróxido de Hidrogênio/análise , Benzidinas/química , Glicemia/análise , Catálise , Glucose/química , Humanos , Peróxido de Hidrogênio/química , Luz , Papel , Peroxidase/química
10.
Oncol Rep ; 41(3): 1487-1496, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30592293

RESUMO

Our previous study demonstrated that bromodomain­containing protein 7 (BRD7) inhibits cell proliferation and tumor growth, restoring the expression of B­cell lymphoma 2 antagonist/killer (Bak) sensitized breast cancer cells to paclitaxel. However, the association between BRD7 and paclitaxel sensitization, as well as BRD7 and Bak in breast cancer remains unknown. In the present study, immunochemical staining was performed to measure the expression of BRD7 and Bak in breast cancer tissues. Cell Counting Kit­8 assay, flow cytometry and tumor xenograft procedures were performed to evaluate the biological role of BRD7 and Bak in breast cancer cells. Western blotting, reverse transcription­quantitative polymerase chain reaction, chromatin immunoprecipitation and luciferase reporter assays were also performed. BRD7 was positively correlated with Bak levels in breast cancer tissues, and the survival rate of patients with low Bak and BRD7 expression was significantly lower than that of patients with high Bak and BRD7 expression. In addition, BRD7 activated Bak promoter activity and induced Bak expression in an indirect manner. Furthermore, ectopic expression of BRD7 inhibited cell proliferation, tumor growth and sensitized cancer cells to paclitaxel, while knockdown of Bak abolished BRD7­mediated inhibitory effects on cell proliferation and paclitaxel sensitization in breast cancer cells whether in vitro and in vivo. The results demonstrated that BRD7 inhibits cell proliferation and sensitizes breast cancer cells to paclitaxel by activating Bak; they also provide promising targets for the diagnosis and treatment of breast cancer.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Neoplasias da Mama Masculina/tratamento farmacológico , Neoplasias da Mama/tratamento farmacológico , Proteínas Cromossômicas não Histona/metabolismo , Paclitaxel/farmacologia , Proteína Killer-Antagonista Homóloga a bcl-2/metabolismo , Antineoplásicos Fitogênicos/uso terapêutico , Apoptose/efeitos dos fármacos , Mama/patologia , Neoplasias da Mama/mortalidade , Neoplasias da Mama/patologia , Neoplasias da Mama Masculina/mortalidade , Neoplasias da Mama Masculina/patologia , Proliferação de Células/efeitos dos fármacos , Resistencia a Medicamentos Antineoplásicos , Feminino , Técnicas de Silenciamento de Genes , Humanos , Células MCF-7 , Masculino , Pessoa de Meia-Idade , Paclitaxel/uso terapêutico , Regiões Promotoras Genéticas/genética , Taxa de Sobrevida , Proteína Killer-Antagonista Homóloga a bcl-2/genética
11.
Platelets ; 30(4): 452-459, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-29617185

RESUMO

Platelet hyperaggregation and hypercoagulation are associated with increase of thrombogenic risk, especially in patients with type 2 diabetes (T2D). High activity of P2Y12 receptor is found in T2D patients, exposing such patients to a prothrombotic condition. P2Y12 is a promising target for antiplatelet, but due to P2Y12 receptor constitutive activation, the clinical practical phenomena such as "clopidogrel resistance" are commonly occurring. In this study, we investigate the role of lncRNA on platelet activation. By lncRNA array, we screened thousands of differentially expressed lncRNA in megakaryocytes from T2D patients and confirmed that lncRNA metallothionein 1 pseudogene 3 (MT1P3) was significantly upregulated in megakaryocytes from T2D patients than in healthy controls. And we further investigate the biofunction of MT1P3 on platelet activation and the regulatory mechanism on p2y12. MT1P3 was positively correlated with p2y12 mRNA levels and promoted p2y12 expression by sponging miR-126. Knockdown of MT1P3 by siRNA reduced p2y12 expression, inhibiting platelet activation and aggregation in diabetes animal model. In conclusion, our findings identify MT1P3 as a key regulator in platelet activation by increasing p2y12 expression through sponging miR-126 under T2D condition. These findings may provide a new insight for managing platelet hyperactivity-related diseases.


Assuntos
Metalotioneína/metabolismo , MicroRNAs/metabolismo , Pseudogenes/genética , RNA Longo não Codificante/metabolismo , Receptores Purinérgicos P2Y12/metabolismo , Animais , Diabetes Mellitus Tipo 2 , Modelos Animais de Doenças , Feminino , Humanos , Masculino , Camundongos , Pessoa de Meia-Idade , Ratos , Ratos Wistar , Transfecção
12.
Cell Death Dis ; 9(5): 519, 2018 05 01.
Artigo em Inglês | MEDLINE | ID: mdl-29725006

RESUMO

The bromodomain-containing protein 7 (BRD7) was first identified as a tumor suppressor in nasopharyngeal carcinoma and has critical roles in cancer development and progression. However, the regulatory roles and mechanisms of BRD7 in cancer metabolism are still unknown. In this study, we demonstrated that BRD7 was lowly expressed in breast cancer tissues and was identified as a poor prognostic factor in breast cancer. Meanwhile, BRD7 could suppress cell proliferation, initiate cell apoptosis and reduce aerobic glycolysis, suggesting that BRD7 plays a tumor suppressive roles in breast cancer. Mechanistically, BRD7 could negatively regulate a critical glycolytic enzyme LDHA through directly interaction with its upstream transcription factor, HIF1α, facilitating degradation of HIF1α mediated by ubiquitin-proteasome pathway. Moreover, restoring the expression of LDHA in breast cancer cells could reverse the effect of BRD7 on aerobic glycolysis, cell proliferation, and tumor formation, as well as the expression of cell cycle and apopotosis related molecules such as cyclin D1, CDK4, P21, and c-PARP both in vitro and in vivo. Taken together, these results indicate that BRD7 acts as a tumor suppressor in breast cancer and represses the glycolysis and tumor progression through inactivation of HIF1α/LDHA transcription axis.

13.
Front Pharmacol ; 9: 82, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29487526

RESUMO

Long non-coding RNA (lncRNA) myocardial infarction associated transcript (MIAT) was recently identified as oncogene in several cancers. However, the role of MIAT on acquired resistance in lung cancer and the underlying mechanisms remain unclear. Here, we showed that the expression of MIAT in lung cancer tissues was upregulated compared with adjacent tissues. LncRNA MIAT expression was associated with tumor size, lymph node metastasis, distant metastasis and TNM stage. Univariate analysis and multivariate analysis revealed that the lncRNA MIAT to be an independent factor for predicating the prognosis of lung cancer patients. Low lncRNA MIAT have longer overall survival time and progression-free survival time than patients with high lncRNA MIAT expression. Moreover, the knockdown of MIAT significantly sensitized PC9 and gefitinib-resistant PC9 cells to gefitinib in vitro and in vivo, and increased the expression of miR-34a and inactivated PI3K/Akt signaling. MIAT interacted with miR-34a and epigenetically controlled the miR-34a expression by hyper-methylating its promotor. Taken together, our findings demonstrated that knockdown of MIAT by siRNA enhances lung cancer cells to gefitinib through the PI3K/Akt signaling pathway by epigenetically regulating miR-34a. Thus, MIAT may be a useful prognostic marker and therapeutic target for lung cancer patients.

14.
J Cardiovasc Transl Res ; 11(1): 22-32, 2018 02.
Artigo em Inglês | MEDLINE | ID: mdl-29313268

RESUMO

It remains unclear if the developmental trajectories of a specific inflammatory biomarker during the acute phase of ST-elevation myocardial infarction (STEMI) provide outcome prediction. By applying latent class growth modeling (LCGM), we identified three distinctive trajectories of CD14++CD16+ monocytes using serial flow cytometry assays from day 1 to day 7 of symptom onset in 96 de novo STEMI patients underwent primary percutaneous coronary intervention. Membership in the high-hump-shaped trajectory (16.8%) independently predicted adverse cardiovascular outcomes during a median follow-up of 2.5 years. Moreover, inclusion of CD14++CD16+ monocyte trajectories significantly improved area under the curve (AUC) when added to left ventricular ejection fraction-based prediction model (ΔAUC = 0.093, P = 0.013). Therefore, CD14++CD16+ monocyte trajectories during STEMI hospitalization are a novel risk factor for post-STEMI adverse outcomes. These results provide the first proof-of-principle evidence in support of the risk stratification role of LCGM-based longitudinal modeling of specific inflammatory markers during acute STEMI.


Assuntos
Hospitalização , Mediadores da Inflamação/imunologia , Monócitos/imunologia , Infarto do Miocárdio com Supradesnível do Segmento ST/imunologia , Idoso , Biomarcadores/sangue , Estudos de Casos e Controles , Feminino , Proteínas Ligadas por GPI/sangue , Proteínas Ligadas por GPI/imunologia , Humanos , Mediadores da Inflamação/sangue , Receptores de Lipopolissacarídeos/sangue , Receptores de Lipopolissacarídeos/imunologia , Masculino , Pessoa de Meia-Idade , Monócitos/metabolismo , Intervenção Coronária Percutânea , Receptores de IgG/sangue , Receptores de IgG/imunologia , Fatores de Risco , Infarto do Miocárdio com Supradesnível do Segmento ST/sangue , Infarto do Miocárdio com Supradesnível do Segmento ST/diagnóstico , Infarto do Miocárdio com Supradesnível do Segmento ST/cirurgia , Fatores de Tempo , Resultado do Tratamento
15.
Oncol Rep ; 39(1): 358-366, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29115527

RESUMO

Short palate, lung and nasal epithelium clone 1 (SPLUNC1) is a tissue-specific gene of nasopharyngeal tissue, and has been recognized as a potential tumor-suppressor gene in nasopharyngeal carcinoma. As a secreted protein, SPLUNC1 plays an important role in innate immunity including antimicrobial and host defense. However, the related immune cells which are regulated by SPLUNC1 remain elusive. In the present study, an acute lung injury (ALI) mouse model was established by administration of lipopolysaccharide (LPS) intraperitoneal injections to wild-type and SPLUNC1-/- mice (5 mg/kg). Pathologic results showed that the SPLUNC1-/- group appeared to have more severe pulmonary damage and infiltrated inflammatory cells compared with the WT group after LPS treatment for 24, 48, 72 and 96 h. The mRNA expression levels of interleukin-6 (IL-6), chemokine (C-C motif) ligand-2 (CCL-2), chemokine (C-C motif) ligand-3 (CCL-3) and chemokine (C-X-C motif) ligand-1 (CXCL-1) in lungs of the SPLUNC1-/- group were higher than these levels in lungs of the WT group at different time points after LPS injection. The percentage of splenic CD11b+Gr-1+ myeloid-derived suppressor cells (MDSCs) in the SPLUNC1-/- mice was higher than this percentage in the WT mice at the time points of 72 and 96 h post LPS injection (P<0.05). These findings demonstrated that SPLUNC1 had a certain protective effect on the LPS-induced ALI mouse model as well as it was found to inhibit the recruitment of MDSCs to the spleen in this model.


Assuntos
Lesão Pulmonar Aguda/patologia , Técnicas de Inativação de Genes , Glicoproteínas/genética , Lipopolissacarídeos/efeitos adversos , Fosfoproteínas/genética , Baço/citologia , Lesão Pulmonar Aguda/induzido quimicamente , Lesão Pulmonar Aguda/genética , Lesão Pulmonar Aguda/imunologia , Animais , Antígeno CD11b/metabolismo , Citocinas/genética , Modelos Animais de Doenças , Glicoproteínas/metabolismo , Humanos , Injeções Intraperitoneais , Pulmão/imunologia , Pulmão/patologia , Camundongos , Células Supressoras Mieloides/imunologia , Fosfoproteínas/metabolismo , Receptores de Superfície Celular/metabolismo , Baço/imunologia , Regulação para Cima
16.
Oncol Res ; 25(7): 1189-1197, 2017 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-28245169

RESUMO

Nasopharyngeal carcinoma (NPC) is a distinct head and neck cancer, which is occurring at a high frequency in Southern China. Emerging studies have shown that long noncoding RNAs (lncRNAs) play a critical role in carcinogenesis and progression. In this study, we established a comprehensive lncRNA profile in NPC and found that 35 lncRNAs were differentially expressed in NPC. We found that LINC0086 was decreased in NPC patient serum samples and tissues. The Kaplan-Meier survival curve showed that patients with high LINC0086 expression had a higher survival rate than those with low LINC0086 expression. LINC0086 expression was associated with NPC histological grade, lymph node metastasis, and clinical stage. Upregulation of LINC0086 inhibited cancer cell proliferation and promoted apoptosis. In addition, upregulation of LINC0086 dramatically decreased the expression of miR-214, an oncogene in several cancers, in C666-1 and HK-1 cells. An miR-214 binding site was found in the 3'-UTR of LINC0086. We also validated that both miR-214 and LINC0086 presented in the RISC complex, demonstrating that LINC0086 could decrease miR-214 expression by directly interacting with miR-214. Furthermore, the suppressive effects of LINC0086 on NPC cell growth were reversed by overexpression of miR-214 in vitro and in vivo. Thus, our study reports a novel mechanism underlying NPC carcinogenesis and provides a potential novel diagnosis and treatment biomarker for NPC.


Assuntos
Carcinoma/genética , Regulação Neoplásica da Expressão Gênica , Genes Supressores de Tumor , MicroRNAs/genética , Neoplasias Nasofaríngeas/genética , Interferência de RNA , RNA Longo não Codificante/genética , Regiões 3' não Traduzidas , Animais , Apoptose/genética , Linhagem Celular Tumoral , Proliferação de Células , Modelos Animais de Doenças , Genes Reporter , Xenoenxertos , Humanos , Masculino , Camundongos , Carcinoma Nasofaríngeo
17.
Oncol Lett ; 13(2): 867-874, 2017 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-28356971

RESUMO

Breast cancer, the second most common cancer worldwide, is the leading cause of cancer-associated mortality in women, accounting for ~15% of all cancer-associated mortalities in women. The development, local invasion and metastasis of breast cancer are associated with the dysregulation and mutation of numerous genes and epigenetic mechanisms, including coding RNA and non-coding RNA, such as microRNAs (miRs/miRNAs). Previous studies have shown a dual-faced role of miR-125b in breast cancer. In the present study, a total of 221 paraffin-embedded breast cancer and 49 paraffin-embedded non-cancerous breast tissue samples were collected. In situ hybridization was used to analyze the expression of miR-125b in the breast cancer tissues. Spearman's rank correlation analysis was used to analyze the expression correlation between miR-125b and human epidermal growth factor 2 (HER2). The overall survival estimates over time were calculated using the Kaplan-Meier method with log-rank test. It was found that miR-125b expression was significantly increased in the breast cancer tissues compared with that in the non-cancerous tissues, and high miR-125b expression indicated a poor prognosis in the breast cancer patients. In addition, miR-125b expression was positively correlated with HER2, but not with progesterone receptor and estrogen receptor. Notably, high miR-125b expression was significantly correlated with tumor size and Tumor-Node-Metastasis stage in the HER2-positive breast cancer patients, along with a poor prognosis. The present study provides clinical data to confirm the oncogenic potential of miR-125b, particularly in HER2-positive human breast cancer. Thus, identification of miR-125b may be a potential molecular biomarker for the prediction of clinical outcomes in breast cancer patients, particularly HER2-positive cases that will receive paclitaxel-based neoadjuvant chemotherapy.

18.
Sci Rep ; 7: 44212, 2017 03 09.
Artigo em Inglês | MEDLINE | ID: mdl-28276503

RESUMO

This work presents an amplified colorimetric biosensor for circulating tumor DNA (ctDNA), which associates the hybridization chain reaction (HCR) amplification with G-Quadruplex DNAzymes activity through triplex DNA formation. In the presence of ctDNA, HCR occurs. The resulting HCR products are specially recognized by one sequence to include one GGG repeat and the other containing three GGG repeats, through the synergetic effect of triplex DNA and asymmetrically split G-Quadruplex forming. Such design takes advantage of the amplification property of HCR and the high peroxidase-like catalytic activity of asymmetrically split G-Quadruplex DNAzymes by means of triplex DNA formation, which produces color signals in the presence of ctDNA. Nevertheless, in the absence of ctDNA, no HCR happens. Thus, no triplex DNA and G-Quadruplex structure is formed, producing a negligible background. The colorimetric sensing platform is successfully applied in complex biological environments such as human blood plasma for ctDNA detection, with a detection limit corresponding to 0.1 pM. This study unambiguously uses triplex DNA forming as the pivot to integrate nucleic acid amplification and DNAzymes for producing a highly sensitive signal with low background.


Assuntos
DNA Tumoral Circulante/sangue , DNA/sangue , Neoplasias/sangue , Técnicas de Amplificação de Ácido Nucleico/métodos , Colorimetria/métodos , Feminino , Humanos , Masculino , Sensibilidade e Especificidade
19.
Nanoscale ; 9(5): 1986-1992, 2017 Feb 02.
Artigo em Inglês | MEDLINE | ID: mdl-28106202

RESUMO

A novel colorimetric sensing strategy for biomolecule assay has been developed, which integrates the signal amplification of the hybridization chain reaction (HCR) with the assembly of gold nanoparticles (AuNPs) through triplex formation. In the presence of targets, the HCR process can be triggered, the HCR products are specifically recognized by triplex-forming oligonucleotide (TFO) functionalized AuNPs, accompanying the aggregation of AuNPs and a dramatic absorbance change at 522 nm. In addition, the DNA hairpin probes can form rigid triplex structures with TFO-functionalized AuNPs in the absence of targets, resulting in a negligible background signal. By taking advantage of this new biosensor platform, a broad range of targets, involving nucleic acids, small molecules and proteins, have been successfully determined with high sensitivity and selectivity, which may hold great potential for practical application.

20.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 32(6): 775-9, 2016 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-27371845

RESUMO

Objective To observe the dynamic changes of the circulating monocyte subsets in C57BL/6 mice fed with high-NaCl diet. Methods Male C57BL/6 mice were randomly divided into three groups: 9, 40 and 80 g/L NaCl groups. Before the treatment and 4, 8 and 12 weeks after the treatment, the cardiac function was dynamically determined by echocardiography and the blood pressure was measured by tail-cuff plethysmography. Flow cytometry analysis of circulating monocyte subsets was performed. HE staining was used to observe cardiac pathological changes at the time of sacrifice. Results Systolic blood pressure significantly increased with the progression of the high-salt diet. Compared with 9 g/L NaCl group, the ejection fraction of the other two groups slightly increased at week 4, followed by a significant decreasing trend up to week 12, in addition, the percentage of Ly6C(high) monocyte subset showed a progressive increase during high-salt feeding and reached a plateau at week 4, and then abruptly went down up to week 12. On the contrary, Ly6C(low) monocyte subset had an opposite trend, whereas Ly6C(int) monocyte subset remained constant. HE staining showed that cardiomyocyte size, as determined by the myocyte cross-sectional area, became enlarged obviously in the latter two groups. Conclusion Circulating monocyte subsets dynamically changed in the mice fed with high-salt diet.


Assuntos
Dieta , Coração/efeitos dos fármacos , Monócitos/efeitos dos fármacos , Cloreto de Sódio/farmacologia , Animais , Antígenos Ly/metabolismo , Pressão Sanguínea/efeitos dos fármacos , Tamanho Celular/efeitos dos fármacos , Relação Dose-Resposta a Droga , Citometria de Fluxo , Coração/fisiologia , Testes de Função Cardíaca , Contagem de Leucócitos , Masculino , Camundongos Endogâmicos C57BL , Monócitos/citologia , Monócitos/metabolismo , Miócitos Cardíacos/citologia , Miócitos Cardíacos/efeitos dos fármacos , Distribuição Aleatória , Cloreto de Sódio/administração & dosagem , Fatores de Tempo
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