Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 11 de 11
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Food Chem ; 305: 125457, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31505414

RESUMO

Taking into account a growing market and small number of articles related to honeydew honey, a metabolomic approach associated with multivariate analysis and modelling was proposed to discriminate five varieties of honey. Advanced analytical techniques were used for determination of 20 elements, 14 carbohydrates and stable carbon isotope ratio. No chemical marker has been found within sugar compounds, but several elements (Ba, Ca, Mg, Sr, Mn, Al, Co, Ni, Se) were marked as characteristic of honey type and allow classification of three botanical origins (Abies alba, Quercus frainetto, Quercus ilex). Sugars turanose, trehalose, arabinose and raffinose, elements Ba, Sr, P, Cd and Se, and δ13C values of honey, have different concentrations in honeys of the same botanical origin but harvested in different season. In addition to a confirmation of authenticity in terms of production, the values of δ13C of protein could be a good indicator of botanical origin.

2.
Food Chem ; 274: 629-641, 2019 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-30372988

RESUMO

Concerning the particular nutritive value of honeydew honey compared to blossom honey, and small number of studies defining botanical origin of honeydew honey, comprehensive analysis of phenolic profile of 64 honeydew honey samples of specific botanical origin was performed. Two advanced techniques of liquid chromatography hyphenated with mass spectrometry were used for identification of a total of 52 compounds and quantification of 25 of them. Pattern recognition analysis applied on data on phenolic compounds content confirmed that quercetin, naringenin, caffeoylquinic acid, hydroxyphenylacetic acid, apigenin and genistein, could be considered as potential markers of botanical origin of honeydew honey. Spectroscopic and electrochemical approaches were applied for the evaluation of the antioxidant capacity. Quercus sps. samples, Quercus frainetto and Quercus ilex, showed high biological activity and specific chemical composition. Additionally, cyclic voltammetry profiles were used for characterization and natural clustering of honeydew honey for the first time.


Assuntos
Antioxidantes/química , Mel/análise , Fenóis/análise , Quercus/química , Cromatografia Líquida de Alta Pressão , Análise por Conglomerados , Técnicas Eletroquímicas , Espectrometria de Massas , Valor Nutritivo , Análise de Componente Principal , Quercus/metabolismo
3.
Med Glas (Zenica) ; 15(2): 139-144, 2018 08 01.
Artigo em Inglês | MEDLINE | ID: mdl-30047542

RESUMO

Aim To determine antimicrobial activity of honey against clinical bacterial strains and their respective reference strains. Methods Twelve samples of Croatian honey from various botanical origin were evaluated for their antimicrobial activity against four clinical antibiotic resistant pathogens and their respective reference strains: Staphylococcus aureus, Acinetobacter baumannii, Pseudomonas aeruginosa and Escherichia coli. Antibacterial susceptibility was checked out by using broth microdilution method and interpreted according to the European Committee for Antimicrobial Susceptibility Testing (EUCAST) recommendations. Results Significant differences in the antibacterial activity of tested honey samples were noticed. Fir honeydew honey and Mint honey showed the best antibacterial potential, while the Locust tree honey, Rapeseed honey and Spring pasture honey expressed the weakest antimicrobial activity. Conclusion Croatian honey, prominently honeydew honey, has the potential to become an important additive to therapeutic techniques available to a medical practitioner against resistant pathogens, but the exact mechanisms of its activity should be investigated further.


Assuntos
Antibacterianos/farmacologia , Bactérias/efeitos dos fármacos , Resistência Microbiana a Medicamentos/efeitos dos fármacos , Mel , Magnoliopsida , Acinetobacter baumannii/efeitos dos fármacos , Croácia , Escherichia coli/efeitos dos fármacos , Humanos , Testes de Sensibilidade Microbiana , Pseudomonas aeruginosa/efeitos dos fármacos , Staphylococcus aureus/efeitos dos fármacos
4.
J Trace Elem Med Biol ; 45: 85-92, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29173488

RESUMO

The concentration of 23 major and trace elements, total phenolic content (TPC) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity were determined in nine samples of strawberry tree honey and compared to other types of unifloral honeys. The most abundant elements in strawberry tree honey were potassium, calcium, magnesium and sodium, ranging between 1276 and 2367, 95.2-154, 14.4-74.4 and 13.4-64.3mg/kg, respectively. Strawberry tree honey had generally higher TPC (range: 0.314-0.522g GA/kg) and DPPH (1.94-4.45mM TE/kg) compared to other analysed unifloral honeys. A strong positive relationship was found between TPC and DPPH, TPC and concentration of homogentisic acid (HGA), chemical marker of strawberry tree honey, and between DPPH and HGA. Regarding daily intake of essential elements, strawberry tree honey can be considered nutritionally richer than the majority of unifloral honeys available in Croatia, while contribution to tolerable intake set for potentially toxic elements was very low, corresponding to pristine areas.


Assuntos
Antioxidantes/análise , Mel/análise , Compostos de Bifenilo/análise , Croácia , Ácido Homogentísico/análise , Picratos/análise , Medição de Risco , Oligoelementos/análise
5.
Water Res ; 119: 160-170, 2017 08 01.
Artigo em Inglês | MEDLINE | ID: mdl-28456079

RESUMO

Bathing water quality is a major public health issue, especially for tourism-oriented regions. Currently used methods within EU allow at least a 2.2 day period for obtaining the analytical results, making outdated the information forwarded to the public. Obtained results and beach assessment are influenced by the temporal and spatial characteristics of sample collection, and numerous environmental parameters, as well as by differences of official water standards. This paper examines the temporal variation of microbiological parameters during the day, as well as the influence of the sampling hour, on decision processes in the management of the beach. Apart from the fecal indicators stipulated by the EU Bathing Water Directive (E. coli and enterococci), additional fecal (C. perfringens) and non-fecal (S. aureus and P. aeriginosa) parameters were analyzed. Moreover, the effects of applying different evaluation criteria (national, EU and U.S. EPA) to beach ranking were studied, and the most common reasons for exceeding water-quality standards were investigated. In order to upgrade routine monitoring, a predictive statistical model was developed. The highest concentrations of fecal indicators were recorded early in the morning (6 AM) due to the lack of solar radiation during the night period. When compared to enterococci, E. coli criteria appears to be more stringent for the detection of fecal pollution. In comparison to EU and U.S. EPA criteria, Croatian national evaluation criteria provide stricter public health standards. Solar radiation and precipitation were the predominant environmental parameters affecting beach water quality, and these parameters were included in the predictive model setup. Predictive models revealed great potential for the monitoring of recreational water bodies, and with further development can become a useful tool for the improvement of public health protection.


Assuntos
Praias , Escherichia coli , Qualidade da Água , Monitoramento Ambiental , Humanos , Água do Mar , Staphylococcus aureus , Fatores de Tempo , Microbiologia da Água
6.
Mar Pollut Bull ; 113(1-2): 438-443, 2016 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-27771099

RESUMO

Bathing Water Directive (2006/7/EC) specifies two reference methods for Escherichia coli detection: ISO 9308-1 and 9308-3. The revised ISO 9308-1 is recommended only for waters with a low bacterial background flora. Considering the extended time needed for analysis and, generally, the lack of experience in using ISO 9308-3 in the Mediterranean, the suitability of ISO 9308-1 for the examination of E. coli in bathing water was evaluated. The present study was aimed at a comparison of data obtained by the reference method in seawater samples (110 beaches, N=477) with data received from six alternative methods. Results show that recently used TSA/TBA method may overestimate E. coli numbers in marine waters. The temperature modified ISO 9308-1 (44°C) did not significantly alter the results, but outperformed the antibiotic supplemented agar at reducing non-E. coli bacteria on the plates, allowing the use of the respective method for monitoring coastal water.


Assuntos
Praias/normas , Monitoramento Ambiental/métodos , Escherichia coli/isolamento & purificação , Recreação , Água do Mar/microbiologia , Microbiologia da Água , Croácia , Mar Mediterrâneo
7.
J Chromatogr Sci ; 54(7): 1077-83, 2016 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-26931733

RESUMO

Propolis is a "natural" remedy with prominent biological activity, which is used as dietary supplement. In the absence of clinical studies that would substantiate these claims, information on the biological activity of propolis is valuable. This study comprises chromatographic, image processing and chemometric approach for phenolic profiling of Serbian, Croatian and Slovenian propolis test solutions. Modern thin-layer chromatography equipment in combination with software for image processing was applied for fingerprinting and data acquisition, whereas the principal component analysis was used as pattern recognition method. Characterization of phenolic profile was performed along with the determination of the botanical and geographical origin of propolis. High-performance thin-layer chromatograms reveal that Central and Southeastern European propolis samples are rich in flavonoids. In addition, phenolic compounds proved to be suitable markers for the determination of European propolis authenticity.


Assuntos
Cromatografia em Camada Delgada/normas , Flavonoides/isolamento & purificação , Processamento de Imagem Assistida por Computador/estatística & dados numéricos , Fenóis/isolamento & purificação , Própole/química , Animais , Abelhas/fisiologia , Croácia , Humanos , Populus , Análise de Componente Principal , Resinas Vegetais/química , Sérvia , Eslovênia , Ceras/química
8.
Arh Hig Rada Toksikol ; 66(4): 233-41, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26751854

RESUMO

Food authenticity in a broader sense means fulfilling chemical and physical criteria prescribed by the proposed legislation. In the case of honey authenticity, two aspects are of major concern: the manufacturing process and the labelling of final products in terms of their geographical and botanical origin. A reliable assessment of honey authenticity has been a longterm preoccupation of chemists-analysts and it usually involves the use of several criteria and chemical markers, as well as a combination of analytical and statistical (chemometric) methods. This paper provides an overview of different criteria and modern methods for the assessment of honey authenticity in the case of a statistically significant number of authentic honey samples of several botanical types from various regions of Serbia.


Assuntos
Análise de Alimentos/métodos , Contaminação de Alimentos/análise , Contaminação de Alimentos/prevenção & controle , Indústria Alimentícia/normas , Rotulagem de Alimentos/normas , Mel/análise , Mel/normas , Qualidade dos Alimentos , Sérvia
9.
Arh Hig Rada Toksikol ; 64(2): 131-9, 2013 Jun.
Artigo em Servo-Croata (Latino) | MEDLINE | ID: mdl-23819941

RESUMO

Phthalates are phthalic acid and aliphatic alcohol esters used as additives to plastic in order to improve its softness, flexibility, and elongation. Phthalates are highly mobile and migrate easily from plastic products into the environment due to their physical and chemical properties. This study briefly describes the characteristics and distribution of phthalates in the environment, their toxic effects on human health, the legislation regarding the maximum allowed concentration of phthalates in drinking water and products intended for infants, as well as the tolerable daily intake. Special attention is given to the methods of determining phthalates and their levels in alcoholic beverages, with an overview of phthalate occurrences and concentrations in plum brandy made in Croatia. A segment on denatured alcohol and illegally marketed alcohol is also included, as well as guidelines for the effective monitoring of the routes of human exposure to phthalates.


Assuntos
Bebidas Alcoólicas/análise , Exposição Ambiental/análise , Monitoramento Ambiental/métodos , Poluentes Ambientais/análise , Ácidos Ftálicos/análise , Croácia , Água Potável/química , Humanos , Lactente , Concentração Máxima Permitida , Abastecimento de Água/legislação & jurisprudência
10.
Mar Pollut Bull ; 73(1): 252-7, 2013 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-23756111

RESUMO

The quality of bathing water is of considerable public importance due to the possibility of fecal contamination. In 2009, Croatia implemented the new European Bathing Water Directive (BWD, 2006/7/EC) establishing stricter microbiological standards for new parameters with new reference methods. This study aims to evaluate the equivalence of different methods according to the old and revised BWD and to provide the possibility of data comparison. Furthermore, the directive requires the establishment of the bathing water profile (BWP) for pollution risk assessment. The estimation of consistency of pollution risk assessment with obtained microbiological results was also performed. Six marine beaches of the Municipality of Rijeka (Croatia) were examined during the 2009 season. Statistical analysis showed equivalence between determination methods for fecal contamination indicators. Based on the current water classification results, the need for correction of estimated pollution risks and recommendations for inclusion of historical microbiological data during BWP enactment was noticed.


Assuntos
Bactérias/crescimento & desenvolvimento , Praias/legislação & jurisprudência , Monitoramento Ambiental/métodos , Água do Mar/microbiologia , Microbiologia da Água/normas , Poluição da Água/legislação & jurisprudência , Bactérias/isolamento & purificação , Praias/normas , Praias/estatística & dados numéricos , Croácia , Monitoramento Ambiental/normas , Medição de Risco , Poluição da Água/análise , Poluição da Água/estatística & dados numéricos
11.
Molecules ; 15(7): 4572-82, 2010 Jun 24.
Artigo em Inglês | MEDLINE | ID: mdl-20657377

RESUMO

A rare sample of maple (Acer spp.) honey from Croatia was analysed. Ultrasonic solvent extraction (USE) using: 1) pentane, 2) diethyl ether, 3) a mixture of pentane and diethyl ether (1:2 v/v) and 4) dichloromethane as solvents was applied. All the extracts were analysed by GC and GC/MS. The most representative extracts were 3) and 4). Syringaldehyde was the most striking compound, being dominant in the extracts 2), 3) and 4) with percentages 34.5%, 33.1% and 35.9%, respectively. In comparison to USE results of other single Croatian tree honey samples (Robinia pseudoacacia L. nectar honey, Salix spp. nectar and honeydew honeys, Quercus frainetto Ten. honeydew as well as Abies alba Mill. and Picea abies L. honeydew) and literature data the presence of syringaldehyde, previously identified in maple sap and syrup, can be pointed out as a distinct characteristic of the Acer spp. honey sample. Headspace solid-phase microextraction (HS-SPME) combined with GC and GC/MS identified benzaldehyde (16.5%), trans-linalool oxide (20.5%) and 2-phenylethanol (14.9%) as the major compounds that are common in different honey headspace compositions.


Assuntos
Acer/química , Mel/análise , Compostos Orgânicos Voláteis/isolamento & purificação , Benzaldeídos/análise , Croácia , Cromatografia Gasosa-Espectrometria de Massas , Álcool Feniletílico , Microextração em Fase Sólida , Solventes , Compostos Orgânicos Voláteis/análise
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA