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1.
Aquat Toxicol ; 212: 20-27, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31039523

RESUMO

Polybrominated diphenyl ethers (PBDEs), a series of highly persistent organic pollutants (POPs), are ubiquitous in marine ecosystems. As key primary producers, microalgae are of great importance on evaluating the environmental outcome of PBDEs pollution. In this study, the toxic mechanisms of BDE-47 on the marine diatom Thalassiosira pseudonana were evaluated by measuring multiple physiological processes. Three concentrations of BDE-47 (25, 15 and 5 µg L-1) were used along with two controls (blank: no BDE-47 or DMSO; negative control: only DMSO). Experiments lasted 144 h (6 days), in which the actual BDE-47 concentrations, cell densities, nutrient (nitrate and phosphate) uptake, pigment compositions, photosynthetic physiology, cell morphology and cellular contents (organic carbon and nitrogen) were measured at 12-48 h intervals. The toxic mechanisms of BDE-47 on T. pseudonana cells were evaluated by measuring multiple physiological processes including photosynthesis, nutrient uptake, cellular material synthesis and cell cycle progressions. The cell divisions of T. pseudonana were severely inhibited by the stress of BDE-47, but the photosynthetic parameters were much less declined and recovered earlier than the cell divisions in the same BDE-47 treatments. The unsuppressed uptake rates of nutrients, increased cell volume and cellular contents indicated the cellular material synthesis proceeded normally. Finally, we found that the cell cycle was arrested in G2/M phase under the stress of BDE-47, we thus concluded that the inhibition of cell divisions by BDE-47 was not due to the lack of energy or cellular materials, where the cell cycle arrest happened; this might be the most important toxicological outcome.


Assuntos
Diatomáceas/efeitos dos fármacos , Diatomáceas/fisiologia , Éteres Difenil Halogenados/toxicidade , Fenômenos Fisiológicos/efeitos dos fármacos , Poluentes Químicos da Água/toxicidade , Carbono/metabolismo , Ciclo Celular/efeitos dos fármacos , Diatomáceas/citologia , Diatomáceas/crescimento & desenvolvimento , Nitrogênio/metabolismo , Fotossíntese/efeitos dos fármacos , Complexo de Proteína do Fotossistema II/metabolismo , Pigmentos Biológicos/metabolismo , Estresse Fisiológico/efeitos dos fármacos
2.
Sci Total Environ ; 628-629: 562-572, 2018 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-29453184

RESUMO

Ecotoxicological methods were applied in the present study, and the marine rotifer Brachionus plicatilis was used as the toxic endpoint to depict what occurred when 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) was combined with solar ultraviolet-B radiation (UV-B). B. plicatilis was exposed to three different combination methods of BDE-47 and UV-B at an equal toxicity ratio, including normal rotifer co-cultured with UV-B-irradiated BDE-47 (known as Method I), UV-B-irradiated rotifer co-cultured with BDE-47 exposure (known as Method II) and normal rotifer co-cultured with the simultaneous addition of BDE-47 and UV-B irradiation (known as Method III). Acute and chronic experiments were preformed to determine the toxicity differentiation according to the growth and reproduction changes in the rotifer. Twenty-four-hour acute experiments showed that the modes of three combined methods changed from antagonism to additive, to synergistic with the concentration/dose increment, and the contribution rates of Method I and Method II to Method III were calculated by approximately 40.4% and 59.6%, respectively. Chronic exposure to either the single stressor or the combination of stressors inhibited the growth and reproduction of the rotifer, demonstrating the inhibition of the population growth rate and the decrease in the larvae production. Three combined groups presented more serious damages compared to groups with single stress exposure, and the ascending sequence of toxicity was Method I

Assuntos
Éteres Difenil Halogenados/toxicidade , Processos Fotoquímicos , Rotíferos/fisiologia , Poluentes Químicos da Água/toxicidade , Animais , Fotólise , Transtornos de Fotossensibilidade , Reprodução
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