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1.
Artigo em Chinês | MEDLINE | ID: mdl-34488267

RESUMO

Objective: To explore the related risk factors of silicosis complicated with chronic obstructive pulmonary disease (COPD) , so as to provide ideas for formulating relevant prevention and treatment measures. Methods: In August 2020, 135 silicosis patients hospitalized in the pneumoconiosis Department of Beidaihe rehabilitation hospital of the emergency management department from August 2019 to July 2020 were selected as the research object. The clinical data of the patients were collected. According to whether they were complicated with COPD, they were divided into Silicosis group (74 cases) and silicosis complicated with COPD group (61 cases) . The physical activity level of the patients was investigated with the international physical activity scale (IPAQ) . Results: Compared with Silicosis group, silicosis complicated with COPD group had higher body mass index (BMI) , current smoking, previous smoking, pneumoconiosis grade Ⅱ, pneumoconiosis grade Ⅲ, insufficient physical activity, family history of respiratory diseases and abnormal C-reactive protein (CRP) (P0.05) . Multivariate logistic regression analysis showed that smoking, family history of respiratory diseases and abnormal CRP were the risk factors of silicosis complicated with COPD (OR=4.704, 2.516, 4.445, P<0.05) . Conclusion: Now smoking, family history of respiratory diseases and abnormal CRP are the possible influencing factors of silicosis complicated with COPD.


Assuntos
Pneumoconiose , Doença Pulmonar Obstrutiva Crônica , Silicose , Humanos , Doença Pulmonar Obstrutiva Crônica/complicações , Estudos Retrospectivos , Silicose/complicações , Fumar
2.
Artigo em Chinês | MEDLINE | ID: mdl-31594117

RESUMO

Objective: To study the causes and influencing factors of no seeking medical treatment among pneumoconiosis patients. Methods: Using stratified sampling method and typical survey method, we carried out the questionnaire survey in nine provinces in China including the east, the medium and the western region using a questionnaire on the seeking medical behavior pf pneumoconiosis patients and the influencing factors. The subjects include occupational pneumoconiosis cases and clinically diagnosed cases and the response rate is 94.3 percent. The data was entered twice with epidate3.1 and error detection and statistical analysis was completed with SPSS 20.0. Chi-square test was used for univariate analysis, and multivariate logistic regression was used for multivariate analysis. Results: One thousand and thirty-seven subjects were investigated with average age 55.9±11.2 years. Seventy percent of them were silicosis and 21.9 percent were coal worker's pneumoconiosis with 67.5 percent of them residing permanently in the countryside, and 37.9 percent of their education background were primary school culture and 33.1 percent of them had junior high school culture. Thirty two point six percent of respondents had no personal income with a median monthly income of 1 200 yuan. Four hundred and thirty four of subjects hadn't seek medical treatment since they got the pneumoconiosis accounting for 41.9 percent with three hundred and thirty seven of them hospitalized directly. The reasons of no seeking medical treatment for the respondents mainly include the self-induction symptoms lighter, the high cost of treatment and cannot claiming the payment of the medical expenses, buying drugs in drugstore, thinking that no medicine can cure pneumoconiosis or no effect, complex procedures, too far away from medical institutions, no unaccompanied, needing a long time or no time, communication disorders, etc. accounting for 44.4 percent, 24.6 percent, 10.9 percent, 9.1 percent, 6.9 percent, 4.4percent, 3.2 percent, 2.9 percent, 1.9 percent, 1.5 percent, respectively. The results of multivariate analysis showed the main characteristics of subjects with restrictions to the outpatient health service utilization are as follows: demographic sociological indicators such as registered permanent residence area is western (OR(western)=2.18, 95%CI:1.38-3.43) , more than seventy five years old (OR(over 75)=6.82, 95%CI:2.04-22.9) , unemployment, temporary or permanent employment (OR (unemployment)=1.90, 95%CI:1.17-3.08; OR(temporary employment)=3.11, 95%CI:1.57-6.14; OR(permanent employment)=2.10, 95%CI:1.18-3.74) , self-rated health score of 50 or above (OR(self-rated-70)=2.04, 95%CI:1.18-3.51; OR(self-rated-90)=3.00, 95%CI:1.97-5.37; OR(self-rated 90)=2.95, 95%CI:1.74-8.07) ; with increase to the outpatient health service utilization are breath with difficulty (OR=0.57, 95%CI:0.41-0.78) , emphysema (OR=0.48, 95%CI:0.26-0.90) , hospitalized with pneumo-coniosis (OR=0.12, 95%CI:0.07-0.20) . Conclusion: Pneumoconiosis patients no covered by injury insurance should be orderly included in the basic medical security system, and be given the medical treatment actively; It should be strengthened the health management for the pneumoconiosis patients and correctly guided the utilization of medical services.


Assuntos
Renda , Aceitação pelo Paciente de Cuidados de Saúde , Pneumoconiose , Adulto , Idoso , China , Emprego , Humanos , Pessoa de Meia-Idade , Inquéritos e Questionários
3.
J Helminthol ; 92(2): 154-160, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-28434412

RESUMO

Toxocara canis is an important but neglected zoonotic parasite, and is the causative agent of human toxocariasis. Chondroitin proteoglycans are biological macromolecules, widely distributed in extracellular matrices, with a great diversity of functions in mammals. However, there is limited information regarding chondroitin proteoglycans in nematode parasites. In the present study, a female-enriched chondroitin proteoglycan 2 gene of T. canis (Tc-cpg-2) was cloned and characterized. Quantitative real-time polymerase chain reaction (qRT-PCR) was employed to measure the transcription levels of Tc-cpg-2 among tissues of male and female adult worms. A 485-amino-acid (aa) polypeptide was predicted from a continuous 1458-nuleotide open reading frame and designated as TcCPG2, which contains a 21-aa signal peptide. Conserved domain searching indicated three chitin-binding peritrophin-A (CBM_14) domains in the amino acid sequence of TcCPG2. Multiple alignment with the inferred amino acid sequences of Caenorhabditis elegans and Ascaris suum showed that CBM_14 domains were well conserved among these species. Phylogenetic analysis suggested that TcCPG2 was closely related to the sequence of chondroitin proteoglycan 2 of A. suum. Interestingly, a high level of Tc-cpg-2 was detected in female germline tissues, particularly in the oviduct, suggesting potential roles of this gene in reproduction (e.g. oogenesis and embryogenesis) of adult T. canis. The functional roles of Tc-cpg-2 in reproduction and development in this parasite and related parasitic nematodes warrant further functional studies.


Assuntos
Proteoglicanas de Sulfatos de Condroitina/genética , Toxocara canis/genética , Transcrição Genética , Animais , Desenvolvimento Embrionário , Feminino , Oogênese , Oviductos , Filogenia , Reação em Cadeia da Polimerase em Tempo Real , Alinhamento de Sequência , Toxocara canis/química , Toxocara canis/fisiologia , Toxocaríase/parasitologia
5.
Artigo em Chinês | MEDLINE | ID: mdl-28241695

RESUMO

Objective: Investigate the pulmonary surfactant autotransfusion effect on the recovery of respiratory function in patients with whole lung lavage, to provide theoretical basis for the clinical application. Methods: We taken 30 patients of pneumoconiosis treated by whole lung lavage as the subjects. We extracted the pulmonary surfactant from lavage fluid, after single postoperative lung lavage for the first time; after one weeks when the second times of lung lavage were performed to the other side of the lung of patients, we put PS into the right side. We taken the patients the second times of lung lavage who were put PS into the right side as returning group, the first times of lung lavage who were not put PS into as on returning group. We observed indi-cators, such as expiratory resistance, respiratory work, lung compliance, airway pressure, PO(2), the pulmonary function recovery time and other indicators, comparing with the changes of pulmonary function before lung la-vage for the first time and at 0、60、90、120 min after the pulmonary surfactant autotransfusion. Results: Com-pared with the no returning group, the expiratory resistance of the returning group decreased significantly at 90 min、120 min after the pulmonary surfactant autotransfusion; the respiratory work and airway pressure of the re-turning group decreased significantly at 60、90、120 min after the pulmonary surfactant autotransfusion, there was statistically significant in the difference between different groups (P<0.05, P<0.01). Compared with the no returning group, the lung compliance and the PO(2) of the returning group increased significantly at 60 min、90 min、120 min after the pulmonary surfactant autotransfusion, there was statistically significant in the difference between different groups (P<0.05, P<0.01). The lung function recovery time of returning group was (155.7 ± 35.2) min, the lung function recovery time of no returning group was (183.71±41.81) min, there was statistical-ly significant in the difference between different groups (P<0.05). Compared with the no returning, there were not statistically significant in the difference of the Heart rate、the systolic blood pressure and the diastolic blood pressure about the returning at 60、90、120 min after the pulmonary surfactant autotransfusion.There was no ad-verse reactions such as pulmonary infection, pulmonary infection and so on. Conclusion: The pulmonary surfac-tant autotransfusion may reduce expiratory resistance, work of breathing, airway pressure; improve lung compliance, alveolar ventilation function; increase oxygen partial pressure and decrease the surgery recovery time in patients with pneumoconiosis.


Assuntos
Líquido da Lavagem Broncoalveolar , Lavagem Broncoalveolar , Complacência Pulmonar , Pneumoconiose/metabolismo , Pneumoconiose/fisiopatologia , Surfactantes Pulmonares , Transfusão de Sangue Autóloga , Humanos , Pneumoconiose/terapia , Testes de Função Respiratória
6.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi ; 35(11): 832-836, 2017 Nov 20.
Artigo em Chinês | MEDLINE | ID: mdl-29316754

RESUMO

Objective: To detect of the components and concentration of the metals and metalloids in the lavage fluid of whole-lung lavage (WLL) of the cases of pneumoconiosis, and analyze the characteristics, and explore the method to sample and process the samples of bronchoalveolar lavage fluid (BAL) . Methods: The samples of urine and serum of three cases of pneumoconiosis were collected before WLL, and the samples of BAL were collected during the WLL from the left and right lungs according to the sequence of four pressured gas flow and five negative pressure drainage. Each of 10ml original samples of WLL was collected firstly, and the left was centrifuged to acquire all the sediment samples and each of 10 ml samples from the centrifuge clear liquids, The components and concentration of the metals and metalloids in the samples were measured by Inductively Coupled Plasma mass spectrometer (ICP-MS) . Results: The average volume of BAL from unilat-eral lung for 3 patients was 10 758.3±1518 ml, and the average recovery rate was 89.7%. The average dry weight of sediment samples of BAL of three cases of pneumoconiosis was 0.292 gram with the right lung sam-ples slightly higher than the left lung samples. The detectable elements from the samples included Barium (Ba) , Strontium (Sr) , Calcium (Ca) , Magnesium (Mg) , Manganum (Mn) , Ferrum (Fe) , Cuprum (Cu) , Zinc (Zn) , Kalium (K) , Natrium (Na) , Selenium (Se) , Silicon (Si) and Uranium (U) . Each of concentration dis-tributions of these elements were not normal. Except for Cuprum, Selenium and Uranium, the concentrations of the other ten elements in the supernatant samples, mixture samples and sediments samples were statistical-ly different with the nonparametric test of Kruskal-Wallis. The concentrations of Natrium, Kalium and Barium in supernatant samples were higher, while the others in precipitation samples were higher. The concentration of elements in the sample from the right lung was slightly higher than that from the left lung, but there was no statistically significant difference (P>0.05) . There were statistical difference in term of element concentration of precipitates samples before and after pressured gas flow (P<0.05) . The concentration of K、Ca、Mg、Si and Se in se-rum, urine, supernatant and sediment samples had a good correlation. The correlation coefficient of Silicon between serum and precipitation was 0.676 and that between urine and precipitation was 0.524. Conclusion: The concentra-tions of the metals and metalloid in sediment samples were more stable than that of supernatant and mixture samples. The sampling of one-side lung lavage fluid was representative in the detection of metals and metalloid in the BAL. It was the best time for sampling sediment from the BAL after the first pressured gas flow among the WLL.


Assuntos
Líquido da Lavagem Broncoalveolar/química , Lavagem Broncoalveolar , Metaloides/análise , Metais/análise , Pneumoconiose/terapia , Humanos , Metaloides/metabolismo , Metais/metabolismo , Espectrofotometria Atômica
7.
Addict Behav ; 26(5): 677-87, 2001.
Artigo em Inglês | MEDLINE | ID: mdl-11676378

RESUMO

This study examined the changes in tobacco sales to minors after active enforcement of merchant compliance to the Synar regulation and the city of Philadelphia Youth Access to Tobacco Ordinance 732. Data for the present study were obtained through Tobacco-free Education and Action Coalition for Health (TEACH) Program in a 5-year, follow-up retail compliance check survey of 1649 stores in 14 cluster areas of Philadelphia, PA. Trend analysis was conducted ofthe sales of tobacco to minors by type of retail outlet, gender, and age of the buyer, and gender, age, and race of the store clerk, and whether restriction policy signs were posted. Analysis indicates that there was a reduction in tobacco sales to minors after implementation of enforcement; sales dropped from 85% in 1994 to 43% in 1998. There were less sales to minors when signs were posted. There were differences in sales if the buyer was asked his or her age and whether the minor was asked to show identification. In addition, the age of the buyer and the brand of cigarettes were associated with sales. Future research should focus on both commercial and social availability and provision of tobacco to minors.


Assuntos
Adolescente , Controle de Medicamentos e Entorpecentes/legislação & jurisprudência , Licenciamento/legislação & jurisprudência , Prevenção do Hábito de Fumar , Fatores Etários , Feminino , Humanos , Masculino , Philadelphia , Fatores Sexuais , Fumar/legislação & jurisprudência , Controle Social Formal
8.
J Sch Health ; 71(6): 218-22, 2001 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-11512488

RESUMO

Teens acquire tobacco from social sources. This study examined factors related to young adults providing tobacco products to minors. Variables such as demographics, rate and method of provision, attitudes toward provision, and youth access restriction laws were measured. A sample of 250 college students completed the survey. About 33.2% of those young adults were asked to provide tobacco to minors, and 30.8% of these students provided it. Students in health-related majors were as likely to be approached and to provide tobacco as were nonhealth majors. Males were more likely to be approached and to provide tobacco to minors than were females. Convenience stores and gas stations were the most popular locations. Young adults favored laws that restrict minors from purchasing tobacco, and their intention to give tobacco in the future was the best predictor of providing tobacco to minors.


Assuntos
Fumar/epidemiologia , Adolescente , Adulto , Análise por Conglomerados , Feminino , Humanos , Masculino , Fumar/legislação & jurisprudência , Fumar/psicologia , Estudantes de Medicina/estatística & dados numéricos
9.
Shanghai Kou Qiang Yi Xue ; 10(3): 233-5, 2001 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-14994002

RESUMO

OBJECTIVE: To study the effects of artificial bone composite of basic fibroblast growth factor(bFGF) with natural non organic bone(NNB)in repairing mandibular defects in rabbits,in order to provide an ideal bone replacement material for clinic. METHODS: 15mm x 6mm bilateral mandibular periosteum bone defects were made surgically in 30 New Zealand adult rabbits and each defect as an experimental unit. The defects were randomly repaired with composite bone, NNB,fresh autogenous bone at 3,6,12 weeks after surgery according to the total random design, blank control group was stayed. Specimens obtained were evaluated with general observation, X-ray as well as computerized histological microscopic analysis. RESULTS: New bone formed and calcified earlier in composite bone and the bone area was greater in the same period than that in NNB. The new bone area in composite bone was still smaller than that of autogenous bone at 6 weeks, while at 12 weeks they had no significant differences. The blank defects were not repaired even in the end. CONCLUSION: Composite bone had sound biocompatibility. It was much better than NNB and can be compared with autogenous bone 12 weeks after operation. So it can be an ideal alternative for bone replacement.

10.
Arch Phys Med Rehabil ; 81(10): 1380-7, 2000 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-11030504

RESUMO

OBJECTIVE: To examine secondary conditions and their relationship to life satisfaction in women with physical disabilities. DESIGN: Survey research. SETTING: Metropolitan Philadelphia. PARTICIPANTS: Women aged 18 to 65 years with self-reported functional limitations associated with neurologic, neuromuscular, brain, sensory, arthritic, spinal cord, orthopedic, or other chronic conditions. MAIN OUTCOME MEASURES: Secondary conditions, health status, functional status, and life satisfaction. RESULTS: In the past year, an average of 12 +/- 6.1 (SD) secondary conditions were experienced. Fatigue, mobility, physical deconditioning, spasticity, and joint pain were reported most frequently, followed by depression, chronic pain, access problems, weight problems, and isolation. In comparing women with multiple sclerosis (MS) and those with other disabling conditions, women with MS had higher secondary conditions problem index (SCPI) scores for bladder, bowel, and sexual dysfunction than women in the mixed disability group. Other secondary conditions with the highest SCPI scores remained similar. Self-reported emotional health status and SCPI scores were the only significant predictors of life satisfaction. CONCLUSION: Secondary conditions influence health status and quality of life for women with physical disabilities. Despite differences among disability groups in terms of the type of secondary conditions, common secondary conditions experienced by most women may be amenable to non-disability-specific health promotion programs.


Assuntos
Doença Crônica/epidemiologia , Comorbidade , Pessoas com Deficiência/estatística & dados numéricos , Atividades Cotidianas , Adulto , Doença Crônica/psicologia , Doença Crônica/reabilitação , Pessoas com Deficiência/psicologia , Feminino , Nível de Saúde , Humanos , Saúde Mental , Pessoa de Meia-Idade , Philadelphia/epidemiologia , Qualidade de Vida
11.
Qual Health Res ; 10(4): 471-89, 2000 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-11010073

RESUMO

This article compares the health perceptions, concerns, and coping strategies among elders in two Asian and Pacific Islander American communities. The qualitative comparison is conducted between elder Chamorros of Guam and elder Chinese of the United States, using a focused ethnography for data collection and the grounded theory method for cross-cultural analysis. The health perceptions of these two communities are manifest in two themes: (a) a sense of holism among body, mind, and spirit and (b) an orientation toward others. The health concerns of these two communities include universal experiences of aging, structural elements within the American health care system, and cultural changes impacting health. A significant coping strategy is adaptation to changes while maintaining continuity. Finally, implications for the delivery of culturally appropriate health care services to Asian and Pacific Islander American elders are discussed.


Assuntos
Adaptação Psicológica , Americanos Asiáticos/psicologia , Atitude Frente a Saúde , Idoso , Envelhecimento , Comparação Transcultural , Guam/etnologia , Necessidades e Demandas de Serviços de Saúde , Serviços de Saúde para Idosos , Nível de Saúde , Humanos , Percepção , Autoimagem , Estados Unidos
12.
Addict Behav ; 25(3): 361-71, 2000.
Artigo em Inglês | MEDLINE | ID: mdl-10890290

RESUMO

This study examined perceived risks and prevalence of alcohol, tobacco, and illicit drug use among ethnic groups of Whites, Blacks, and Hispanics; compared the extent to which drug use varied among different ethnic groups; explored whether or not there were any age and gender differences in drug use among the ethnic groups; and made implications of the findings. Comparative analyses were conducted by using chi2 to determine the association of independent variables (ethnicity, age, and gender) with the dependent variables (alcohol, tobacco, and illicit drug use) during lifetime, past year, and past month. Data were derived from the 1997 and 1996 National Household Survey on Drug Abuse. Our findings indicated that there are differences in perceived risks and reported use among ethnic groups, as well as in preferences for particular drugs. Whites are the least likely to perceive risks for substance abuse, however, they have the highest prevalence of lifetime drug use of most types of drugs (with the exception of crack cocaine and heroin), as compared with Blacks and Hispanics. The examination of past month categories of drug use shows that Whites had the highest prevalence rates of alcohol and smokeless tobacco use. Hispanics were more likely to binge, and reported higher prevalence of heavy alcohol use. Blacks were more likely than Whites or Hispanics to use crack and heroin in their lifetime. Implications were made for future research and drug prevention in ethnic populations.


Assuntos
Atitude Frente a Saúde , Grupos Étnicos/psicologia , Transtornos Relacionados ao Uso de Substâncias/etnologia , Transtornos Relacionados ao Uso de Substâncias/psicologia , Adolescente , Adulto , Criança , Feminino , Humanos , Masculino , Prevalência , Medição de Risco , Fatores de Risco , Índice de Gravidade de Doença , Transtornos Relacionados ao Uso de Substâncias/diagnóstico , Inquéritos e Questionários , Estados Unidos/epidemiologia
13.
J Allied Health ; 29(2): 64-70, 2000.
Artigo em Inglês | MEDLINE | ID: mdl-10874332

RESUMO

This study examined the extent to which cultural, socioeconomic, and systemic factors impeded access to and utilization of health services among a convenience sample of 52 Chinese immigrants living in metropolitan Houston. The subjects, of differing levels of socioeconomic status, were 25 years old or older. Methods used for data collection included participant observation, face-to-face interview, and case study. A semistructured interview instrument with open- and closed-ended questions was administered. A pilot study and expert reviews were conducted for content and face validity. Cultural and socioeconomic factors were found to be strongly associated with access to and utilization of health services. Mainland Chinese and Taiwanese shared similar cultural dilemmas as they sought health care, including communication difficulties, beliefs about health, health care, and illness, and mistrust in Western health care. Although families played important roles in health decisions and choices of services, social class differences also appeared to affect utilization. For example, more affluent Taiwanese than Mainland Chinese were apt to carry health insurance and use Western systems. The findings suggest a need to improve services to the Chinese community through family-centered and community-based approaches adapted to Chinese culture.


Assuntos
Americanos Asiáticos , Necessidades e Demandas de Serviços de Saúde , Serviços de Saúde/estatística & dados numéricos , Adulto , China/etnologia , Comportamento de Escolha , Características Culturais , Coleta de Dados , Feminino , Conhecimentos, Atitudes e Prática em Saúde , Acesso aos Serviços de Saúde , Humanos , Masculino , Fatores Socioeconômicos , Taiwan/etnologia , Texas
14.
Space Med Med Eng (Beijing) ; 13(1): 52-4, 2000 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-12214612

RESUMO

OBJECTIVE: To investigate the effects of heat and noise environments on lipid peroxidation of erythrocyte membrane in pilots. METHOD: Twenty-four pilots and twenty-one ground personnel (control group) served as the subjects. The pilots performed flying in heat and noise environments. Glutathione peroxidase (GSHpx) and malondialdehyde [correction of malondiadehyde] (MDA) levels in erythrocyte membrane were determined before flying (6:00 a.m.), immediately after flying (12:00 a.m.) and 8 hours after flying (8:00 p.m.) respectively with a spectrophotometer. RESULT: Immediately after flying, GSHpx activity in pilot's erythrocyte membrane decreased significantly as compared with that in control group. Immediately after flying and 8 h after flying, MDA contents in pilots increased significantly as compared with that of control group. CONCLUSION: Heat and noise environments might induce increase of lipid peroxidation reaction and decrease of antioxidant ability.


Assuntos
Membrana Eritrocítica/fisiologia , Temperatura Alta , Peroxidação de Lipídeos/fisiologia , Ruído Ocupacional , Adulto , Medicina Aeroespacial , Aviação , Membrana Eritrocítica/enzimologia , Membrana Eritrocítica/metabolismo , Glutationa Peroxidase/sangue , Glutationa Peroxidase/metabolismo , Humanos , Malondialdeído/sangue , Malondialdeído/metabolismo
15.
J Community Health ; 24(6): 421-37, 1999 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-10593423

RESUMO

This study examined the use of traditional and Western health services by Chinese immigrants, as well as the cultural and socioeconomic factors affecting health-seeking behaviors and health service utilization patterns among the study population from the perspectives of consumers and Chinese health care providers. Two instruments were used for data collection. The first, a consumer instrument, was designed for interviews of service recipients; the second, a health provider instrument, was designed to elicit information from traditional and Western providers. A few topics in the former instrument were cross-examined from the perspectives of health care providers. The investigation employed a combination of qualitative and quantitative research methods for data collection. Qualitative ethnographic methods used included: (1) participant-observation, (2) face-to-face interview, and (3) case study. To complement the qualitative data, structured quantitative survey were conducted with all selected informants. A total of 105 informants participated in the study: 75 Chinese consumers and 30 Chinese health professionals. The latter group was composed of Western physicians and traditional practitioners. Results revealed several patterns of health-seeking and service utilization behaviors among the Chinese of Houston and Los Angeles. These included high rates of self-treatment and home remedies (balanced diets and other alternative medicines); medium rates of utilization of integrated Western and traditional health services, including travel to country of origin for care; and low rates of exclusive utilization of Western or traditional Chinese treatments.


Assuntos
Serviços de Saúde/estatística & dados numéricos , Medicina Tradicional Chinesa , Adulto , Atitude Frente a Saúde , China/etnologia , Terapias Complementares/estatística & dados numéricos , Diversidade Cultural , Coleta de Dados , Emigração e Imigração , Feminino , Médicos Graduados Estrangeiros , Nível de Saúde , Humanos , Renda , Seguro Saúde , Los Angeles , Masculino , Projetos Piloto , Texas
16.
Prep Biochem Biotechnol ; 29(4): 371-83, 1999 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-10548253

RESUMO

High Performance Centrifugal Partition Chromatography (HPCPC) is a practical and suitable method, particularly on the preparative scale, for the separation of biomolecules such as proteins, enzymes, etc. Aqueous two-phase system is also very attractive for the isolation of biomolecules. Aqueous polymer phase system composed of polyethylene glycol 6000-potassium phosphate has been used for the countercurrent chromatographic separation of bovine serum albumin (BSA) and lysozyme using HPCPC. The separation of BSA and lysozyme under various conditions such as various flow rates, rotational speeds, pH of the solvent, and the retention of stationary phase has been studied in the present investigation. The baseline separation of BSA and lysozyme has been also observed. The results of this study demonstrate that HPCPC is useful for separation of proteins with aqueous two-phase systems.


Assuntos
Cromatografia Líquida/métodos , Fosfatos/química , Polietilenoglicóis/química , Compostos de Potássio/química , Soroalbumina Bovina/isolamento & purificação , Concentração de Íons de Hidrogênio , Água
17.
Space Med Med Eng (Beijing) ; 12(6): 397-400, 1999 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-12432882

RESUMO

Objective. To determine the effect of heat and noise on erythrocyte membrane ATPase activities in pilots during flying. Method. Twenty-four pilots performing bombing for 3 h (45-53 degrees C, 122-97 dB in the cabin) served as the subjects. 21 ground personnel served as control (27 degrees C in the room). Blood samples were taken from both groups before flying (6:00 a.m.), and immediately (12:00 a.m.) and 8 h (8:00 p.m.) after flying. Na(+)-K+ ATPase, and Ca2(+)-Mg2+ ATPase activities in erythrocyte membrane were determined with colorimetry. Result. The Na(+)-K+ ATPase activity in erythrocyte membrane at 6:00 a.m. in pilots was higher than that in control group at the same time (P<0.01). The Ca2(+)-Mg2+ ATPase activities in erythrocyte membrane at 12:00 a.m. and 8:00 p.m. in pilots were significantly higher, compared with those in control group at the same time (P<0.01). Conclusion. The ATPase values obtained in our study were all within normal range, and the daytime variation of both groups are the same. Exposure of human body to heat and noise for long time may be harmful, the higher ATPase activity is, the more catabolism of ATP will be. ATP exhaustion will lead to Ca2+ overload in erythrocyte thus stiffen the red cell membrane.


Assuntos
ATPase de Ca(2+) e Mg(2+)/metabolismo , Membrana Eritrocítica/enzimologia , Temperatura Alta , Ruído , ATPase Trocadora de Sódio-Potássio/metabolismo , Adulto , Medicina Aeroespacial , Aeronaves , Aviação , China , Humanos , Militares
18.
J Sch Health ; 68(4): 131-6, 1998 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-9644604

RESUMO

Alcohol is the most commonly abused substance among adolescents in the United States. Adolescent females are recognized as one group at risk for giving birth to babies with fetal alcohol syndrome (FAS). Sixth through eighth grade Native Americans were surveyed about their attitudes toward and knowledge of FAS risk factors and prevention strategies. Data revealed that 52% of students drank alcohol prior to the survey. Though sexually active, students lacked knowledge about the relationship between alcohol and FAS. The study revealed 1) limited prevention programs in middle schools and 2) the most influential factor in determining attitudes and decisions about alcohol use was the immediate family. Students felt FAS prevention is an important topic in school health education, noting the important role peers play in teaching and role modeling. Various strategies incorporating music and communication technology such as videotape and computer-assisted interactive tools into prevention materials are discussed.


Assuntos
Comportamento do Adolescente , Transtornos do Espectro Alcoólico Fetal/prevenção & controle , Conhecimentos, Atitudes e Prática em Saúde , Índios Norte-Americanos/psicologia , Adolescente , Consumo de Bebidas Alcoólicas/epidemiologia , Distribuição de Qui-Quadrado , Feminino , Grupos Focais , Humanos , Masculino , Fatores de Risco , Inquéritos e Questionários
19.
Clin Orthop Relat Res ; (256): 229-37, 1990 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-2114247

RESUMO

Implantation of porcine bone morphogenetic protein (pBMP) in the muscle induces differentiation of mesenchymal-type cells and results in endochondral bone formation. pBMP was isolated from porcine demineralized bone matrix and purified by hydroxyapatite chromatography, Sephadex G75 gel filtration, preparative sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), preparative isoelectric focusing (IEF), and chromatofocusing fast protein liquid chromatography (FPLC). Porcine BMP has an MW of 26 K and a range of pI from 4.65 to 4.73 determined by SDS-PAGE and IEF, respectively. Reconstitution with the citrate buffer supernatant fraction enables as little as 50 micrograms of the soluble pBMP fractions to induce osteogenesis in an in vivo assay. Chemical modification studies indicate that the osteoinductive potential of the pBMP molecule depends on tyrosine, carboxyl groups, and disulfide bonds and can be increased by modification of sulfhydryl groups. Modification of arginine and tryptophan has no effect on bioactivity. By pepsin-limited proteolysis, fragments of pBMP with an MW of 6-14 K show definite, although reduced, BMP activity.


Assuntos
Osso e Ossos/metabolismo , Substâncias de Crescimento/isolamento & purificação , Proteínas/isolamento & purificação , Animais , Bioensaio , Matriz Óssea/efeitos dos fármacos , Matriz Óssea/metabolismo , Proteínas Morfogenéticas Ósseas , Osso e Ossos/efeitos dos fármacos , Substâncias de Crescimento/farmacologia , Métodos , Camundongos , Peso Molecular , Osteogênese/efeitos dos fármacos , Pepsina A/farmacologia , Proteínas/efeitos dos fármacos , Proteínas/farmacologia , Suínos
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