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1.
Artigo em Inglês | MEDLINE | ID: mdl-35944085

RESUMO

OBJECTIVE: Lower limb stiffness strategies and work mode changes between young and older adults during stair descent are unclear. This study investigated the effect of aging on the lower limb stiffness, moments and joint work mode during stair descent. DESIGN: Twenty young adults and Twenty older adults were recruited from the local community for stair descent test. Kinematics and kinetics data were collected by Vicon system and Kistler force plate. The lower limb stiffness, moments and work mode were calculated and assess between groups. RESULTS: No significant differences in gait parameters were detected between groups. Compared with young adults, older adults have decreased leg stiffness, knee and ankle stiffness, increased peak hip extension moment, hip stiffness and ankle work contribution. CONCLUSION: The older adults actively reduce the lower limb stiffness to reduce the risk of injury during stair descent. The hip joint strategy reduces the risk of forwarding falls, and ankle joint compensation work mode to make up for the lack of knee extension strength. This provides a reference for the focus of exercise intervention and rehabilitation strategies for older adults.

2.
Stem Cell Res Ther ; 13(1): 403, 2022 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-35932064

RESUMO

BACKGROUND: Mesenchymal stem cell (MSC) therapy has been shown to be a promising option for liver fibrosis treatment. However, critical factors affecting the efficacy of MSC therapy for liver fibrosis remain unknown. Follistatin-like 1 (FSTL1), a TGF-ß-induced matricellular protein, is documented as an intrinsic regulator of proliferation and differentiation in MSCs. In the present study, we characterized the potential role of FSTL1 in MSC-based anti-fibrotic therapy and further elucidated the mechanisms underlying its action. METHODS: Human umbilical cord-derived MSCs were characterized by flow cytometry. FSTL1low MSCs were achieved by FSTL1 siRNA. Migration capacity was evaluated by wound-healing and transwell assay. A murine liver fibrotic model was created by carbon tetrachloride (CCl4) injection, while control MSCs or FSTL1low MSC were transplanted via intravenous injection 12 weeks post CCl4 injection. Histopathology, liver function, fibrosis degree, and inflammation were analysed thereafter. Inflammatory cell infiltration was evaluated by flow cytometry after hepatic nonparenchymal cell isolation. An MSC-macrophage co-culture system was constructed to further confirm the role of FSTL1 in the immunosuppressive capacity of MSCs. RNA sequencing was used to screen target genes of FSTL1. RESULTS: FSTL1low MSCs had comparable gene expression for surface markers to wildtype but limited differentiation and migration capacity. FSTL1low MSCs failed to alleviate CCl4-induced hepatic fibrosis in a mouse model. Our data indicated that FSTL1 is essential for the immunosuppressive action of MSCs on inflammatory macrophages during liver fibrotic therapy. FSTL1 silencing attenuated this capacity by inhibiting the downstream JAK/STAT1/IDO pathway. CONCLUSIONS: Our data suggest that FSTL1 facilitates the immunosuppression of MSCs on macrophages and that guarantee the anti-fibrotic effect of MSCs in liver fibrosis.


Assuntos
Proteínas Relacionadas à Folistatina , Transplante de Células-Tronco Mesenquimais , Células-Tronco Mesenquimais , Animais , Folistatina/efeitos adversos , Folistatina/metabolismo , Proteínas Relacionadas à Folistatina/genética , Humanos , Cirrose Hepática/induzido quimicamente , Cirrose Hepática/genética , Cirrose Hepática/terapia , Células-Tronco Mesenquimais/metabolismo , Camundongos
3.
Pharmacol Biochem Behav ; 219: 173428, 2022 Jul 19.
Artigo em Inglês | MEDLINE | ID: mdl-35868565

RESUMO

Discovery of interventions that delay or minimize age-related diseases is arguably the major goal of aging research. Conversely discovery of interventions based on phenotypic screens have often led to further elucidation of pathophysiological mechanisms. Although most hypotheses to explain lifespan focus on cell-autonomous processes, increasing evidence suggests that in multicellular organisms, neurons, particularly nutrient-sensing neurons, play a determinative role in lifespan and age-related diseases. For example, protective effects of dietary restriction and inactivation of insulin-like signaling increase lifespan and delay age-related diseases dependent on Creb-binding protein in GABA neurons, and Nrf2/Skn1 in just 2 nutrient-sensing neurons in C. elegans. Screens for drugs that increase lifespan also indicate that such drugs are predominantly active through neuronal signaling. Our own screens also indicate that neuroactive drugs also delay pathology in an animal model of Alzheimer's Disease, as well as inhibit cytokine production implicated in driving many age-related diseases. The most likely mechanism by which nutrient-sensing neurons influence lifespan and the onset of age-related diseases is by regulating metabolic architecture, particularly the relative rate of glycolysis vs. alternative metabolic pathways such as ketone and lipid metabolism. These results suggest that neuroactive compounds are a most promising class of drugs to delay or minimize age-related diseases.

4.
Eur J Dermatol ; 32(2): 244-248, 2022 04 01.
Artigo em Inglês | MEDLINE | ID: mdl-35866900

RESUMO

Background: Laser treatment for congenital melanocytic nevi (CMN) is controversial. Correlations between anatomical distribution of facial CMN and efficacy of laser treatment have not been characterized. Materials & Methods: A total of 90 facial CMN treated with ablative laser (Er:YAG or CO2 laser) were retrospectively reviewed. Clinical assessments were completed by at least two plastic surgeons using the Investigator's Global Assessment (IGA) score. Results: The mean IGA score was 4.37. The IGA scores recorded at the periorbital, temple/forehead, nose, cheek and lip/chin areas were 3.68, 5.88, 4.63, 4.52 and 4.92, respectively (p = 0.03). Moreover, facial CMN with uniform pigment distribution showed higher IGA scores than those with a non-uniform pigment distribution. Thickening, hairiness and age at first treatment were not statistically correlated with laser efficacy. Conclusion: Correlations between anatomical distribution and the efficacy of laser treatment are described for the first time. The subdivided anatomical distribution of facial CMN can yield meaningful information on predicting the efficacy of laser treatment.


Assuntos
Terapia a Laser , Nevo Pigmentado , Neoplasias Cutâneas , Humanos , Imunoglobulina A , Nevo Pigmentado/congênito , Nevo Pigmentado/cirurgia , Estudos Retrospectivos , Neoplasias Cutâneas/congênito , Neoplasias Cutâneas/radioterapia , Neoplasias Cutâneas/cirurgia
5.
Stem Cells ; 2022 Jun 27.
Artigo em Inglês | MEDLINE | ID: mdl-35759955

RESUMO

Quiescent hair follicle stem cells (HFSCs) reside in specialized bulge niche where they undergo activation and differentiation upon sensing niche-dependent signals during hair follicle (HF) homeostasis and wound repair. The underlying mechanism of HFSCs and bulge niche maintenance is poorly understood. Our previous study has reported that a transcription factor, forkhead box P1 (Foxp1), functions to maintain the quiescence of HFSCs. Here, we further discovered that forkhead box P4 (Foxp4), a close family member of Foxp1, had similar expression profiles in various components of HFs and formed a complex with Foxp1 in vitro and in vivo. The HF-specific deficiency of Foxp4 resulted in the precocious activation of HFSCs during hair cycles. In contrast to single Foxp1 or Foxp4 conditional knockout (cKO) mice, Foxp1/4 double cKO exerted an additive effect in the spectrum and severity of phenotypes in HFSC activation, hair cycling acceleration and hair loss, coupled with remarkable downregulation of fibroblast growth factor 18 (Fgf18) and bone morphogenetic protein 6 (Bmp6) expression in bulge cells. In addition, the double KO of Foxp1/4 induced the apoptosis of K6-positive (K6+) inner bulge cells, a well-established stem cell (SC) niche, thus resulting in the destruction of the bulge SC niche and recurrent hair loss. Our investigation reveals the synergistic role of Foxp1/4 in sustaining K6+ niche cells for the quiescence of HFSCs.

6.
ACS Omega ; 7(20): 16980-16989, 2022 May 24.
Artigo em Inglês | MEDLINE | ID: mdl-35647439

RESUMO

To facilitate the flame retardancy of phenolic resin (PF), a series of novel flame retardants with nano-SiO2, melamine, and aluminum diethylphosphinate (ADP) were freshly prepared and tested. A thermogravimetric analysis, cone calorimeter, and scanning electron microscopy were employed to determine the thermal decomposition, flame retardancy, combustion properties, and structure of the carbon residue layer of PF. The pyrolysis kinetic parameters of modified PF were then computed, and the pyrolysis process was appraised. The results indicated that when 1.5 wt % of nano-SiO2, 3 wt % of melamine, and 15 wt % of ADP were added to PF, the limiting oxygen index value reached 39.6%, and UL-94 passed the V-0 level. A substantial synergistic effect was also observed. The thermogravimetric analysis revealed that the char residue at 800 °C reached 59.93 wt %. Furthermore, in the cone calorimeter test, the total thermal release and thermal release rate decreased to 30.7 MJ/m2 and 105.7 kW/m2, respectively.

7.
Front Genet ; 13: 902541, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35719395

RESUMO

Transposable elements (TEs) are mobile genetic elements that can randomly integrate into other genomic sites. They have successfully replicated and now occupy around 40% of the total DNA sequence in humans. TEs in the genome have a complex relationship with the host cell, being both potentially deleterious and advantageous at the same time. Only a tiny minority of TEs are still capable of transposition, yet their fossilized sequence fragments are thought to be involved in various molecular processes, such as gene transcriptional activity, RNA stability and subcellular localization, and chromosomal architecture. TEs have also been implicated in biological processes, although it is often hard to reveal cause from correlation due to formidable technical issues in analyzing TEs. In this review, we compare and contrast two views of TE activity: one in the pluripotent state, where TEs are broadly beneficial, or at least mechanistically useful, and a second state in human disease, where TEs are uniformly considered harmful.

8.
PLoS Biol ; 20(6): e3001682, 2022 06.
Artigo em Inglês | MEDLINE | ID: mdl-35771762

RESUMO

Around 60% of in vitro fertilized (IVF) human embryos irreversibly arrest before compaction between the 3- to 8-cell stage, posing a significant clinical problem. The mechanisms behind this arrest are unclear. Here, we show that the arrested embryos enter a senescent-like state, marked by cell cycle arrest, the down-regulation of ribosomes and histones and down-regulation of MYC and p53 activity. The arrested embryos can be divided into 3 types. Type I embryos fail to complete the maternal-zygotic transition, and Type II/III embryos have low levels of glycolysis and either high (Type II) or low (Type III) levels of oxidative phosphorylation. Treatment with the SIRT agonist resveratrol or nicotinamide riboside (NR) can partially rescue the arrested phenotype, which is accompanied by changes in metabolic activity. Overall, our data suggests metabolic and epigenetic dysfunctions underlie the arrest of human embryos.


Assuntos
Embrião de Mamíferos , Fertilização In Vitro , Embrião de Mamíferos/metabolismo , Epigênese Genética , Histonas/metabolismo , Humanos , Zigoto/metabolismo
9.
Phytomedicine ; 102: 154145, 2022 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-35567994

RESUMO

BACKGROUND: Anthocyanins are a kind of flavonoids and natural water-soluble pigments, which endow fruits, vegetables, and plants with multiple colors. They are important source of new products with prebiotic activity. However, there is no systematic review documenting prebiotic activity of anthocyanins and their structural analogues. This study aims to fill this gap in literature. PURPOSE: The objective of this review is to summarize and evaluate the prebiotic activity of anthocyanin's, and discuss the physical and molecular modification methods to improve their biological activities. STUDY DESIGN AND METHODS: In this review, the databases (PubMed, Google Scholar, Web of Science, Researchgate and Elsevier) were searched profoundly with keywords (anthocyanin's, prebiotics, probiotics, physical embedding and molecular modification). RESULTS: A total of 34 articles were considered for reviewing. These studies approved that anthocyanins play an important role in promoting the proliferation of probiotics, inhibiting the growth of harmful bacteria and improving the intestinal environment. In addition, physical embedding and molecular modification have also been proved to be effective methods to improve the prebiotic activity of anthocyanins. Anthocyanins could promote the production of short chain fatty acids, accelerate self degradation and improve microbial related enzyme activities to promote the proliferation of probiotics. They inhibited the growth of harmful bacteria by inhibiting the expression of harmful bacteria genes, interfering with the role of metabolism related enzymes and affecting respiratory metabolism. They promoted the formation of a complete intestinal barrier and regulated the intestinal environment to keep the body healthy. Physical embedding, including microencapsulation and colloidal embedding, greatly improved the stability of anthocyanins. On the other hand, molecular modification, especially enzymatic modification, significantly improved the biological activities (antioxidant, prebiotic activity and so on) of anthocyanins. CONCLUSION: All these research results displayed by this review indicate that anthocyanins are a useful tool for developing prebiotic products. The better activities of the new anthocyanins formed by embedding and modification may make them become more effective raw materials. Our review provides a scientific basis for the future research and application of anthocyanins.


Assuntos
Antocianinas , Probióticos , Antocianinas/farmacologia , Flavonoides/análise , Frutas/química , Prebióticos/análise
10.
Plants (Basel) ; 11(10)2022 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-35631732

RESUMO

The plant-specific homeodomain zipper family (HD-ZIP) of transcription factors plays central roles in regulating plant development and environmental resistance. HD-ZIP transcription factors IV (HDZ IV) have been involved primarily in the regulation of epidermal structure development, such as stomata and trichomes. In our study, we identified nine HDZ IV-encoding genes in Cannabis sativa L. by conducting a computational analysis of cannabis genome resources. Our analysis suggests that these genes putatively encode proteins that have all the conserved domains of HDZ IV transcription factors. The phylogenetic analysis of HDZ IV gene family members of cannabis, rice (Oryza sativa), and Arabidopsis further implies that they might have followed distinct evolutionary paths after divergence from a common ancestor. All the identified cannabis HDZ IV gene promoter sequences have multiple regulation motifs, such as light- and hormone-responsive elements. Furthermore, experimental evidence shows that different HDZ IV genes have different expression patterns in root, stem, leaf, and flower tissues. Four genes were primarily expressed in flowers, and the expression of CsHDG5 (XP_030501222.1) was also correlated with flower maturity. Fifty-nine genes were predicted as targets of HDZ IV transcription factors. Some of these genes play central roles in pathogen response, flower development, and brassinosteroid signaling. A subcellular localization assay indicated that one gene of this family is localized in the Arabidopsis protoplast nucleus. Taken together, our work lays fundamental groundwork to illuminate the function of cannabis HDZ IV genes and their possible future uses in increasing cannabis trichome morphogenesis and secondary metabolite production.

11.
QJM ; 2022 Apr 19.
Artigo em Inglês | MEDLINE | ID: mdl-35438153

RESUMO

OBJECTIVE: The purposes of this survey were to show the current situation of oncology critical care medicine in China by questionnaire, to understand the resource distribution of oncology critical care medicine, and to analyze and evaluate the existing resources and reserve capacity of oncology critical care medicine in China. METHODS: We conducted the survey mainly in the form of an online questionnaire. The Committee of Cancer Critical Care Medicine of the Chinese Anticancer Association (CACA) initiated the survey on November 1st, 2017, and 36 member hospitals nationwide participated in the survey. The questionnaire included ten items: investigator information, hospital information, general information of oncology critical care department, staffing of oncology critical care department, management in oncology critical care department, technical skills in oncology critical care department, patient source in oncology critical care department, equipment configuration in oncology critical care department, special skills in oncology critical care department and summary of the information. RESULTS: The survey results included information from 28 member units, all of which were tertiary hospitals, distributed in 20 provinces and 4 direct-controlled municipalities. The results are as follows. (1) The total ratio of beds in the oncology critical care department to hospital beds was 1.06%, and the average number of beds in the oncology critical care department was 16.36. (2) The ratio of physicians in the oncology critical care department to beds was approximately 0.62:1, and the ratio of nurses to beds was approximately 1.98:1. (3) According to the census of the population and gross domestic product (GDP) of different regions conducted by the State Statistics Bureau in 2017, the ratio of beds in the oncology critical care department for tumor patients to population was 4.55 beds per 10 million people, and the ratio of beds in the oncology critical care department to GDP was 8.00 beds per RMB 100 billion, on average. (4) According to the requirements of the guidelines for the development and management of critical care medicine in China, the facilities in departments of oncology critical care medicine meet the requirements, and the technical skills of medical staff are competent. CONCLUSION: The development of oncology critical care in China is becoming better, but there is still a certain gap compared with the intensive care unit (ICU) standards in China and the average level of the nationwide. The development of oncology critical care medicine is urgent.

12.
Med Biol Eng Comput ; 60(6): 1763-1774, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35469375

RESUMO

Although some studies tried to identify risk factors for COVID-19, the evidence comparing COVID-19 and community-acquired pneumonia (CAP) is inconclusive, and CAP is the most common pneumonia with similar symptoms as COVID-19. We conducted a case-control study with 35 routine-collected clinical indicators and demographic factors to identify predictors for COVID-19 with CAP as controls. We randomly split the dataset into a training set (70%) and testing set (30%). We built Explainable Boosting Machine to select the important factors and built a decision tree on selected variables to interpret their relationships. The top five individual predictors of COVID-19 are albumin, total bilirubin, monocyte count, alanine aminotransferase, and percentage of monocyte with the importance scores ranging from 0.078 to 0.567. The top systematic predictors for COVID-19 are liver function, monocyte increasing, plasma protein, granulocyte, and renal function (importance scores ranging 0.009-0.096). We identified five combinations of important indicators to screen COVID-19 patients from CAP patients with differentiating abilities ranging 83.3-100%. An online predictive tool for our model was published. Certain clinical indicators collected routinely from most hospitals could help screen and distinguish COVID-19 from CAP. While further verification is needed, our findings and predictive tool could help screen suspected COVID-19 cases.


Assuntos
COVID-19 , Pneumonia , COVID-19/diagnóstico , Estudos de Casos e Controles , Humanos , Aprendizado de Máquina , Pneumonia/diagnóstico , Fatores de Risco
13.
Photobiomodul Photomed Laser Surg ; 40(3): 159-162, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-35298284

RESUMO

Background: Vascular-targeted photodynamic therapy (PDT) is an effective alternative treatment choice for port-wine stains (PWSs). The histological characteristics of PWSs after PDT treatment have not yet been reported. Objective: To investigate the morphological features of PWSs treated by PDT and define the histopathological characteristics of PWS that achieve clinical cure. Methods: Thirteen patients with facial PWSs, who presented with complete regressive PWS lesions after a mean of 4.38 (standard deviation = 4.907) sessions of PDT. Post-treatment biopsy samples were obtained from each patient. The number of blood vessels, vascular diameter, and depth were measured and compared in all samples of PDT-regressive sites, PDT-resistant sites, and normal skin. Results: Within the 7-year follow-up after PDT, there was no recurrence in the regression area of PDT. In the PDT-regressive sites, within 800 µm of the dermal-epidermal junction, the dilated vessels were occluded and remained fissure-like after PDT. Conclusions: When the vascular lesions within 800 µm of the dermal-epidermal junction were closed after PDT, a stable clinical cure (no recurrence) was achieved.


Assuntos
Fotoquimioterapia , Mancha Vinho do Porto , Seguimentos , Humanos , Mancha Vinho do Porto/tratamento farmacológico , Resultado do Tratamento
14.
Sci Rep ; 12(1): 4791, 2022 03 21.
Artigo em Inglês | MEDLINE | ID: mdl-35314755

RESUMO

The lung microbiota have been found to be substantially altered in numerous pulmonary disorders, and crosstalk between the host pathophysiology and lung microbiota plays critical roles in the regulation of disease states. The aim of this study was to investigate dynamic changes in the lung microbiota during different stages of acute lung injury and acute respiratory distress syndrome (ALI/ARDS). Rats receiving an intraperitoneal administration of lipopolysaccharide (LPS) were sacrificed at 12 and 48 h after injection, and the hematological parameters, serum cytokine levels, and histological characteristics of the lung tissue and lung microbiota were assessed. After LPS injection, along with fluctuations of systemic cytokine levels and the onset and regression of pulmonary edema, the diversity, components, and functionalities of the pulmonary microbiota underwent significant dynamic changes. The volatility of the α-diversity indices narrowed after LPS injection, and the indices significantly decreased 48 h later. The abundance of 18 genera and functionality of adenosine triphosphate-binding cassette (ABC) transporters, pentose phosphate, and bacterial chemotaxis pathways were found to significantly differ between specified time points. Several significant correlations between the components and functionalities of the lung microbiota and indicative symptoms of ALI/ARDS were also observed. Brevibacterium was correlated with cytokines tumor necrosis factor (TNF)-α, interleukin (IL)-10, and IL-6 and with hematological percentage of neutrophils (NEU%); Wnt, Notch, and chronic myeloid leukemia signaling pathways were correlated with IL-1ß; mitogen-activated protein kinase (MAPK) signaling pathway-yeast was correlated with IL-10; and the pathways of ascorbate and aldarate metabolism and basal transcription factors were correlated with platelet-related indicators. The correlations between the lung microbiota and indicative symptoms of ALI/ARDS identified in this study support further investigation into the underlying mechanism of host-microbiota interactions during lung injury and repair.


Assuntos
Lesão Pulmonar Aguda , Microbiota , Síndrome do Desconforto Respiratório , Lesão Pulmonar Aguda/metabolismo , Animais , Citocinas/metabolismo , Lipopolissacarídeos/metabolismo , Pulmão/patologia , Ratos , Fator de Necrose Tumoral alfa/metabolismo
16.
Physiol Meas ; 43(3)2022 04 04.
Artigo em Inglês | MEDLINE | ID: mdl-35213844

RESUMO

Objective. The arrhythmia identification method based on the U-net has the potential for fast application. The RR-intervals have been proven to improve the performance of single-heartbeat identification methods. However, because both the heartbeats number and location in the input of the U-net are unfixed, the approach based on the U-net cannot use RR-intervals directly. To solve this problem, we proposed a novel method. The proposed method also can identify heartbeats of four classes, including non-ectopic (N), supraventricular ectopic beat (SVEB), ventricular ectopic beat (VEB), and fusion beat (F).Approach. Our method consists of the pre-processing and the two-stage identification framework. In the pre-processing part, we filtered input signals with a band-pass filter and created the auxiliary waveforms by RR-intervals. In the first stage of the framework, we designed a network to handle input signals and auxiliary waveforms. We proposed a masking operation to separate the input signal into two signals according to the result of the network. The first signal contains heartbeats of SVEB and VEB. The second signal includes heartbeats of N and F. The second stage consists of two networks and can further identify the heartbeats of SVEB, VEB, N, and F from these two signals.Main result. We validated our method on the MIT-BIH arrhythmia database with the inter-patient model. For classes N, SVEB, VEB, and F, our approach achieved F1 scores of 98.26, 68.61, 95.99, and 47.75, respectively.Significance. Our method not only can effectively utilize RR intervals but also can identify multiple arrhythmias.


Assuntos
Eletrocardiografia , Complexos Ventriculares Prematuros , Algoritmos , Eletrocardiografia/métodos , Frequência Cardíaca , Humanos , Redes Neurais de Computação , Processamento de Sinais Assistido por Computador
17.
Hepatol Commun ; 6(6): 1403-1412, 2022 06.
Artigo em Inglês | MEDLINE | ID: mdl-35182047

RESUMO

The diagnosis of primary biliary cholangitis (PBC) in patients with seropositive anti-mitochondrial antibody (AMA) but normal alkaline phosphatase (ALP) depends on a liver biopsy. We aimed to reveal potential serum biomarkers that could suggest the necessity of a liver biopsy in such patients. Retrospective analysis was performed. Subjects who were treatment naive with seropositive AMA but normal ALP and who underwent at least one liver biopsy between 2008 and 2020 were included in this study. Histologic biopsies were evaluated by two experienced pathologists blinded to the serum tests. A total of 115 patients who were treatment naive were included in this study. Of these, 77 patients (67%) exhibited histologic PBC features and nonspecific histologic features were found in the remaining 38 (33%) patients. Multivariate analysis suggested that baseline serum immunoglobulin M (IgM) >0.773 × upper limit of normal (ULN) (P < 0.001) and age >42 years (P = 0.002) were associated with the diagnosis of PBC through liver biopsies. A significant decrease in the median levels of gamma-glutamyl transpeptidase (GGT) and IgM was found in 54 patients with PBC who received ursodeoxycholic acid (UDCA). Conclusion: For patients who were treatment naive with seropositive AMA but normal ALP, baseline serum IgM >0.773 × ULN and age >42 years were the factors that strongly suggested a diagnosis of PBC. In these patients receiving UDCA, a dynamic monitoring of GGT and IgM might be helpful in evaluating therapeutic responses.


Assuntos
Cirrose Hepática Biliar , Adulto , Fosfatase Alcalina , Biomarcadores , Humanos , Imunoglobulina M/uso terapêutico , Cirrose Hepática Biliar/diagnóstico , Estudos Retrospectivos , Ácido Ursodesoxicólico/uso terapêutico , gama-Glutamiltransferase
18.
Plant Physiol Biochem ; 173: 14-24, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-35091187

RESUMO

In the present study, we studied the effects of gibberellic acid (GA) on chlorophyll and carotenoid metabolites and related gene expression during the regreening process in Valencia orange fruits (Citrus sinensis Osbeck). During the regreening, fruits treated with GA turned green much faster than those of the control. Compared with untreated fruits, chlorophyll accumulation was induced and the content of carotenoids (ß-cryptoxanthin, all-trans-violaxanthin, and 9-cis-violaxanthin) was decreased by the GA treatment. Chlorophyll and carotenoid contents following GA treatment appeared to be highly regulated at the gene transcription level. Correspondingly, the up-regulation of chlorophyll biosynthesis genes (CitGGDR, CitCHL27, CitPORA, and CitCAO) and down-regulation of degradation genes (CitCLH1, CitSGR, CitPPH, CitPAO, and CitRCCR) led to the increase of chlorophyll contents, and the down-regulation of carotenoid biosynthesis genes (CitPSY, CitPDS, CitZDS, CitLCYb2, and CitHYb) led to the decrease of carotenoid contents. These observations indicated that GA acted as a crucial regulator in the regreening process of citrus fruits.


Assuntos
Citrus sinensis , Citrus , Carotenoides , Clorofila , Citrus/metabolismo , Frutas/metabolismo , Regulação da Expressão Gênica de Plantas , Giberelinas , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo
19.
Curr Opin Insect Sci ; 50: 100878, 2022 04.
Artigo em Inglês | MEDLINE | ID: mdl-35093582

RESUMO

Global pest invasions have significantly increased in recent years. These invasions together with climate warming directly impact agriculture. Tropical climates feature extreme weather events, including high temperatures and seasonal droughts. Thus, successful invasive pests in tropics have to adapt to these extreme climate features. The intrinsic factors relevant to tropical invasion of insects have been explored in many studies, but the knowledge is rather dispersed in contemporary literature. Here, we reviewed the potential biophysical characters of successful invasive pests' adaption to tropical environments including [1] inherent high basal stress tolerance and advanced life-history performances [2], phenotypic plasticity [3], rapid evolution to environmental stress, polyphagy, diverse reproductive strategies and high fecundity. We summarised how these traits and their interactive effects enhance pest invasions in the tropics. Comprehensive understanding of how these characters facilitate invasion improves models for predicting ecological consequences of climate change on invasive pest species for improved pest management.


Assuntos
Mudança Climática , Plásticos , Animais , Insetos , Espécies Introduzidas , Clima Tropical
20.
Immunology ; 165(1): 3-21, 2022 01.
Artigo em Inglês | MEDLINE | ID: mdl-34558663

RESUMO

An autoimmune disease is an inappropriate response to one's tissues due to a break in immune tolerance and exposure to self-antigens. It often leads to structural and functional damage to organs and systemic disorders. To date, there are no effective interventions to prevent the progression of autoimmune diseases. Hence, there is an urgent need for new treatment targets. TRPM7 is an enzyme-coupled, transient receptor ion channel of the subfamily M that plays a vital role in pathologic and physiologic conditions. While TRPM7 is constitutively activated under certain conditions, it can regulate cell migration, polarization, proliferation and cytokine secretion. However, a growing body of evidence highlights the critical role of TRPM7 in autoimmune diseases, including rheumatoid arthritis, multiple sclerosis and diabetes. Herein, we present (a) a review of the channel kinase properties of TRPM7 and its pharmacological properties, (b) discuss the role of TRPM7 in immune cells (neutrophils, macrophages, lymphocytes and mast cells) and its upstream immunoreactive substances, and (c) highlight TRPM7 as a potential therapeutic target for autoimmune diseases.


Assuntos
Doenças Autoimunes/etiologia , Doenças Autoimunes/metabolismo , Autoimunidade , Imunomodulação , /metabolismo , Canais de Cátion TRPM/genética , Canais de Cátion TRPM/metabolismo , Animais , Doenças Autoimunes/diagnóstico , Doenças Autoimunes/terapia , Biomarcadores , Suscetibilidade a Doenças , Desenvolvimento de Medicamentos , Regulação da Expressão Gênica/efeitos dos fármacos , Humanos , Sistema Imunitário/citologia , Sistema Imunitário/efeitos dos fármacos , Sistema Imunitário/imunologia , Sistema Imunitário/metabolismo , Imunomodulação/efeitos dos fármacos , Ativação do Canal Iônico/efeitos dos fármacos , Especificidade de Órgãos/efeitos dos fármacos , Especificidade de Órgãos/genética , Especificidade de Órgãos/imunologia , Inibidores de Proteínas Quinases/química , Inibidores de Proteínas Quinases/farmacologia , Transdução de Sinais/efeitos dos fármacos , Relação Estrutura-Atividade , Canais de Cátion TRPM/química
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