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1.
Spectrochim Acta A Mol Biomol Spectrosc ; 264: 120278, 2022 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-34438116

RESUMO

An analytical formula that relates the molecular constants of the Herzberg expression and experimental transition lines is developed herein with a difference algebraic approach (DAA) model. Based on the data-driven strategy, the DAA model is able to deal with the tiny uncertainties that exhibit behind the experimental transition lines, which is applied to the P branch emission spectra of some first overtone bands of the ground electronic state of 12C16O. The relationship can be used to generate transition lines with sufficient accuracy, as evident from the high J of agreement with the HITRAN database, Velichko data, Goorvitch data and quantum-mechanical data. In addition, line intensities, absorption oscillator strengths and Einstein A coefficients of these lines, which are introduced to enhance the dataset and are in good agreement with those of other authors, are also reported to validate our results. These various comparative results show that the proposed data-driven strategy based on the DAA model is expecting to be a good algorithm that relies on relatively limited data for training.

2.
Chemosphere ; 286(Pt 3): 131965, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34449324

RESUMO

The production and degradation of plastic remains can result in nanoplastics (NPs) formation. However, insufficient information regarding the environmental behaviors of NPs impedes comprehensive assessment of their significant threats. In this study, the transport behavior of unmodified NPs (PSNPs), carboxyl-modified NPs (PSNPs-COOH), and amino-modified NPs (PSNPs-NH2) was investigated using column experiments in the presence and absence of goethite (GT) and diethylhexyl phthalate (DEHP). Quantum chemical computation was performed to reveal the transport mechanisms. The results showed that GT decreased the transport of NPs and the presence of DEHP decreased it further. Van der Waals forces and small electrostatic interactions coexisted between the PSNPs and GT and caused deposition. Ligand exchange caused greater deposition of PSNPs-COOH on GT-coated sand than that of PSNPs. Although hydrogen bonding existed between the DEHP and NPs with functional groups, an increase in the positive charge and chemical heterogeneity of the collector was the main reason for DEHP promoting the deposition of NPs. Because of low absolute negative zeta potential values, PSNPs-NH2 was sensitive to chemical heterogeneity, and thus fully deposited (over 96.9%) in GT and GT-DEHP-coated columns. Generally, the deposition of NPs due to chemical heterogeneity was more significant than that due to the formation of chemical bonds and van der Waals, electrostatic, and hydrogen interactions. Our results highlight that the surface charge and functional groups significantly influence the transport behaviors of NPs and elucidate the fate of NPs in the terrestrial environment.


Assuntos
Dietilexilftalato , Microplásticos , Plásticos , Poliestirenos , Areia
3.
Neural Regen Res ; 17(4): 806-811, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-34472479

RESUMO

Pain is one of the manifestations of hip disorder and has been proven to lead to the remodeling of somatotopic map plasticity in the cortex. However, most studies are volume-based which may lead to inaccurate anatomical positioning of functional data. The methods that work on the cortical surface may be more sensitive than those using the full brain volume and thus be more suitable for map plasticity study. In this prospective cross-sectional study performed in Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, China, 20 patients with osteonecrosis of the femoral head (12 males and 8 females, aged 56.80 ± 13.60 years) and 20 healthy controls (9 males and 11 females, aged 54.56 ± 10.23 years) were included in this study. Data of resting-state functional magnetic resonance imaging were collected. The results revealed that compared with healthy controls, compared with the healthy controls, patients with osteonecrosis of the femoral head (ONFH) showed significantly increased surface-based regional homogeneity (ReHo) in areas distributed mainly in the left dorsolateral prefrontal cortex, frontal eye field, right frontal eye field, and the premotor cortex and decreased surface-based ReHo in the right primary motor cortex and primary sensory cortex. Regions showing significant differences in surface-based ReHo values between the healthy controls and patients with ONFH were defined as the regions of interests. Seed-based functional connectivity was performed to investigate interregional functional synchronization. When the areas with decreased surface-based ReHo in the frontal eye field and right premotor cortex were used as the regions of interest, compared with the healthy controls, the patients with ONFH displayed increased functional connectivity in the right middle frontal cortex and right inferior parietal cortex and decreased functional connectivity in the right precentral cortex and right middle occipital cortex. Compared with healthy controls, patients with ONFH showed significantly decreased cortical thickness in the para-insular area, posterior insular area, anterior superior temporal area, frontal eye field and supplementary motor cortex and reduced volume of subcortical gray matter nuclei in the right nucleus accumbens. These findings suggest that hip disorder patients showed cortical plasticity changes, mainly in sensorimotor- and pain-related regions. This study was approved by the Medical Ethics Committee of Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine (approval No. 2018-041) on August 1, 2018.

4.
Bioengineered ; 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34724866

RESUMO

Ovarian cancer (OC) is the leading cause of death from gynaecological cancer. In this study, we aimed to explore the role and potential mechanism of LIMD2 during the progression of OC. The expression of LIMD2 was analyzed by GEPIA (Gene Expression Profiling Interactive Analysis) database. Western blot and real-time PCR were applied to detect the gene expression of LIMD2 in OC cell lines. Cell counting kit-8 (CCK-8) assay, transwell, wound healing assays, and tumor xenograft experiments were used to evaluate the function of LIMD2 in vitro and vivo. Further, the LIMD2-associated pathways in OC were predicted by RNA-seq analysis, and the involvement of the corresponding cell signaling activities were confirmed by Western blot. We found that LIMD2 was high expressed in OC. Additionally, we found that silencing of LIMD2 inhibited OC cell proliferation in vitro and reduced the growth of its xenograft tumors. Moreover, knockdown of LIMD2 significantly decreased the migration of OC cells. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that pathways regulating extracellular matrix (ECM)-receptor interactions and focal adhesion signaling, were deregulated by LIMD2. Particularly, we confirmed that reducing LIMD2 could decrease the expression of Focal adhesion kinase (FAK) pathway related molecules. In conclusion, LIMD2 promotes the proliferation and invasion of ovarian cancer in vitro and in vivo, potentially through regulating the focal adhesion signaling pathway.

5.
Front Immunol ; 12: 769047, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34777388

RESUMO

Pancreatic ductal adenocarcinoma (PDAC) is a malignant tumor characterized by rapid progression, early metastasis, high recurrence, and limited responsiveness to conventional therapies. The 5-year survival rate of PDAC is extremely low (<8%), which lacks effective prognostic evaluation indicators. In this study, we used xCell to analyze infiltrating immune cells in a tumor and through the univariate and multivariate Cox analyses screened out two prognosis-related immune cells, CD4+TN and common lymphoid progenitor (CLP), which were used to construct a Cox model and figure out the risk-score. It was found that the constructed model could greatly improve the sensitivity of prognostic evaluation, that the higher the risk-score, the worse the prognosis. In addition, the risk-score could also identify molecular subtypes with poor prognosis and immunotherapy sensitivity. Through transcriptome and whole-exome sequencing analysis of PDAC dataset from The Cancer Genome Atlas (TCGA), it was found that copy number deletion and low expression of CCL19 might be crucial factors to affect the risk-score. Lastly, validation of the above findings was confirmed not only in Gene Expression Omnibus (GEO) datasets but also in our PDAC patient samples, Peking2020 cohort.

6.
Opt Express ; 29(21): 32892-32899, 2021 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-34809111

RESUMO

Herein, we report on the experimental observations and a quantitative determination of the laser-induced frequency shift (LIFS) in the photoassociation (PA) spectra of spinor Bose-Einstein condensate of sodium. Our investigations revealed a nonlinear dependence of the LIFS on the intensity of PA laser. By developing a model within the quadratic Stark effect, we simulate the experimental results via a theoretical model that confirms the former. The experimental observations and the theoretical analysis can further improve the accuracy of investigations on important molecular properties and on preparation of specific molecular states, with possible applications in various key fields.

7.
Front Plant Sci ; 12: 770736, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34804103

RESUMO

Nitrogen is essential for plant growth and yield, and it is, therefore, crucial to increase the nitrogen-use efficiency (NUE) of crop plants in fields. In this study, we measured four major low-nitrogen-induced growth response (LNGR) agronomic traits (i.e., plant height, tiller number, chlorophyll content, and leaf length) of the 225-rice-variety natural population from the Rice 3K Sequencing Project across normal nitrogen (NN) and low nitrogen (LN) environments. The LNGR phenotypic difference between NN and LN levels was used for gene analysis using a genome-wide association study (GWAS) combined with 111,205 single-nucleotide polymorphisms (SNPs) from the available sequenced data from the 3K project. We obtained a total of 56 significantly associated SNPs and 4 candidate genes for 4 LNGR traits. Some loci were located in the candidate regions, such as MYB61, OsOAT, and MOC2. To further study the role of candidate genes, we conducted haplotype analyses to identify the elite germplasms. Moreover, several other plausible candidate genes encoding LN-related or NUE proteins were worthy of mining. Our study provides novel insight into the genetic control of LNGR and further reveals some related novel haplotypes and potential genes with phenotypic variation in rice.

8.
Research (Wash D C) ; 2021: 9754145, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34806019

RESUMO

Faradaic electrode materials have significantly improved the performance of membrane capacitive deionization, which offers an opportunity to produce freshwater from seawater or brackish water in an energy-efficient way. However, Faradaic materials hold the drawbacks of slow desalination rate due to the intrinsic low ion diffusion kinetics and inferior stability arising from the volume expansion during ion intercalation, impeding the engineering application of capacitive deionization. Herein, a pseudocapacitive material with hollow architecture was prepared via template-etching method, namely, cuboid cobalt hydroxide, with fast desalination rate (3.3 mg (NaCl)·g-1 (h-Co(OH)2)·min-1 at 100 mA·g-1) and outstanding stability (90% capacity retention after 100 cycles). The hollow structure enables swift ion transport inside the material and keeps the electrode intact by alleviating the stress induced from volume expansion during the ion capture process, which is corroborated well by in situ electrochemical dilatometry and finite element simulation. Additionally, benefiting from the elimination of unreacted bulk material and vertical cobalt hydroxide nanosheets on the exterior surface, the synthesized material provides a high desalination capacity (117 ± 6 mg (NaCl)·g-1 (h-Co(OH)2) at 30 mA·g-1). This work provides a new strategy, constructing microscale hollow faradic configuration, to further boost the desalination performance of Faradaic materials.

9.
J Org Chem ; 2021 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-34747599

RESUMO

A palladium-catalyzed base-free decarbonylative borylation of aryl anhydrides has been developed. Catalyst system consisting of Pd(OAc)2/dppb enables readily available aryl anhydrides to be employed as electrophiles for the synthesis of versatile arylboronate esters via O-C(O) bond activation and decarbonylation. This method is characterized by an excellent functional group tolerance and broad substrate scope, using bench stable aryl anhydrides as aryl electrophiles in C-B bond formation. Mechanistic studies and functionalization of late-stage pharmaceutical molecules are disclosed.

10.
ACS Appl Mater Interfaces ; 13(45): 53850-53858, 2021 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-34738780

RESUMO

Designing electrode materials with excellent comprehensive properties was of top priority in promoting development of flow electrode capacitive deionization (FCDI). To date, most FCDI studies involved the application and modification of carbon-based materials, which suffered the contradiction between rheological behavior and electrochemical performance. In this study, a Na+ superionic conductor (NASICON) sodium vanadium fluorophosphate@reduced graphene oxide (NVOPF@rGO) was synthesized and applied as a flow electrode in FCDI. Benefiting from the confinement effect of the three-dimensional (3D) reduced graphene oxide (rGO) network, thin and uniform NVOPF nanosheets formed and provided abundant active sites for adsorbing Na+. Moreover, the interconnected rGO network formed a 3D conductive network for Na+ and electron transport. Compared with an activated carbon (AC)-AC system (AC was used as an anode and a cathode), a NVOPF@rGO-AC system (NVOPF@rGO was used as a cathode and AC was used as an anode) exhibited preferable dispersibility and stability of electrode dispersion, lower internal resistance, higher desalination rate, and lower energy consumption. Besides, the average salt adsorption rate (ASAR) reached 5.32 µg·cm-2·min-1 by adjusting the concentration of the electrode (4.73 wt %), the flow rate of the electrode (25 mL·min-1), and the operation voltage (1.6 V). This study demonstrated the potential of faradic flow electrodes for promoting the development and application of FCDI.

11.
J Hematol Oncol ; 14(1): 196, 2021 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-34794493

RESUMO

Myeloid-derived suppressor cells (MDSCs) are derived from bone marrow progenitor cells commonly, which is a heterogeneous cell group composed of immature granulocytes, dendritic cells, macrophages and early undifferentiated bone marrow precursor cells. Its differentiation and immunosuppressive function are regulated by complex network signals, but the specific regulation mechanisms are not yet fully understood. In this study, we found that in mouse of Lewis lung cancer xenograft, long non-coding RNA Snhg6 (lncRNA Snhg6) was highly expressed in tumor-derived MDSCs compared with spleen-derived MDSCs. LncRNA Snhg6 facilitated the differentiation of CD11b+ Ly6G- Ly6Chigh monocytic MDSCs (Mo-MDSCs) rather than CD11b+ Ly6G+ Ly6Clow polymorphonuclear MDSCs (PMN-MDSCs), but did not affect the immunosuppressive function of MDSCs. Notably, lncRNA Snhg6 could inhibit the expression of EZH2 by ubiquitination pathway at protein level rather than mRNA level during the differentiation of mouse bone marrow cells into MDSCs in vitro. EZH2 may be an important factor in the regulation of lncRNA Snhg6 to promote the differentiation of Mo-MDSCs. So what we found may provide new ideas and targets for anti-tumor immunotherapy targeting MDSCs.

12.
Dalton Trans ; 50(45): 16685-16693, 2021 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-34758054

RESUMO

In this study, we reported the covalent post-synthetic modification (PSM) of a luminescent complex to achieve aggregation-induced emission (AIE), prepared using the Schiff base reaction of TPE-CHO and HLC-NH2, denoted by HLC-NH2-TPE. HLC-NH2 formed a 2D luminescent complex which was constructed using 4,4'-diamino-[1,1'-biphenyl]-2,2'-dicarboxylic acid and zinc ions via a solvothermal reaction. HLC-NH2-TPE inherited the luminescence properties of HLC-NH2 and exhibited noticeable AIE properties in response to environmental viscosities and temperature changes. Interestingly, HLC-NH2-TPE displayed a time-dependent luminescence conversion phenomenon in a mixed solution of DMF/H2O (v : v/1 : 9).

13.
Chemosphere ; : 132794, 2021 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-34742765

RESUMO

Recently, the microplastics, as well as the compound pollution of heavy metals and antibiotics, in the aqueous environment have attracted increasing attention, but studies on the behavior of heavy metals on aged microplastics in antibiotics-heavy metals coexisting system are limited. Herein, to explore the impact of antibiotics on the adsorption of heavy metals by aged microplastics, the adsorption behavior of Cu(II) and Cr(VI) on aged polystyrene (PS) and polyvinyl chloride (PVC) were investigated. The results presented that ciprofloxacin (CIP) had negative and positive impacts on the adsorption of Cu(II) and Cr(VI) by aged microplastics, respectively. Moreover, the existence of CIP seemed to have no evident effect on the type of adsorption isotherm model and kinetic model in most adsorption systems of Cu(II). The negative impact of CIP on adsorption of Cu(II) may result from the competition adsorption and high steric hindrance effect, while non-specific interactions between neutral antibiotic-heavy metal complexes and the hydrophobic surface of aged microplastics as well as CIP as bridges influenced positively on adsorption of Cr(VI) on aged microplastics. This work was concerned with the interaction between binary pollutants and aging microplastics to clarify the risks of microplastics in the aqueous environment.

15.
BMC Genomics ; 22(Suppl 5): 544, 2021 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-34789143

RESUMO

BACKGROUND: With the rapid increase in the amount of Protein-Protein Interaction (PPI) data, the establishment of an event-centered PPI ontology that contains temporal and spatial vocabularies is urgently needed to clarify PPI biological annotations. In this paper, we propose a precisely designed schema - PPIO (PPI Ontology) for representing the biological context of PPIs. RESULTS: Inspired by the event model and the distinct characteristics of PPI events, PPIO consists of six core aspects of the information required for reporting a PPI event, including the interactor (who), the biological process (when), the subcellular location (where), the interaction type (how), the biological function (what) and the detection method (which). PPIO is implemented through the integration of appropriate terms from the corresponding vocabularies/ontologies, e.g., Gene Ontology, Protein Ontology, PSI-MI/MOD, etc. To assess PPIO, an approach based on PPIO in developed to extract PPI biological annotations from an open standard corpus "BioCreAtIvE-PPI". The experiment results demonstrate PPIO's high performance, a precision of 0.69, a recall of 0.72 and an F-score of 0.70. CONCLUSIONS: PPIO is a well-constructed essential ontology in the interpretation of PPI biological context. The results of the experiments conducted on the BioCreAtIvE corpus demonstrate that PPIO is able to facilitate PPI annotation extraction from biomedical literature effectively and enrich essential annotation for PPIs.


Assuntos
Mapeamento de Interação de Proteínas , Proteínas , Ontologia Genética
16.
Hum Exp Toxicol ; : 9603271211052991, 2021 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-34752167

RESUMO

Background: Nicotinamide phosphoribosyltransferase (NAMPT) catalyzes the rate-limiting step in the salvage pathway of mammalian nicotinamide adenine dinucleotide (NAD+) biosynthesis. Through its NAD+-biosynthetic activity, NAMPT is able to regulate the development of hepatic steatosis and inflammation induced by diet or alcohol. However, the roles NAMPT plays in the development of liver fibrosis remain obscure. Purpose: To investigate the roles of NAMPT-mediated NAD+ biosynthesis in hepatic stellate cell (HSC) activation and liver fibrosis. Research Design: Realtime RT-PCR and western blot analyses were performed to analyze the expression of profibrogenic genes. Sirius red staining was conducted to examine the fibrosis in liver. Mouse liver fibrosis was induced by intraperitoneal injection of carbon tetrachloride (CCl4) 2 times a week for 6 weeks. Adenovirus-mediated NAMPT overexpression or nicotinamide mononucleotide (NMN) administration was carried out to study the effects of elevation of NAD+ levels on protecting CCl4-induced liver fibrosis in mice. LX2 cells or primary HSCs were used to study the role of NAMPT overexpression or NMN treatment in reducing profibrogenic gene expression in vitro. ResultsCCl4 administration suppresses NAMPT expression in liver and reduces hepatic NAD+ content. Tgfß1 treatment decreases intracellular NAD+ levels and NAMPT expression in LX2 cells. Adenovirus-mediated NAMPT overexpression augments liver NAD+ levels, inhibits HSC activation and alleviates CCl4-induced liver fibrosis in mice. Administration of NMN also suppresses HSC activation and protects against CCl4-induced liver fibrosis in mice. Conclusions: NAMPT-mediated NAD+ biosynthesis inhibits HSC activation and protects against CCl4-induced liver fibrosis.

17.
Bioengineered ; 2021 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-34753388

RESUMO

Spinal cord injury (SCI) is a traumatic disease resulting in neuronal injury. circRNAs are closely associated with human diseases. Nevertheless, the potential mechanism by which circRNAs impact SCI remains to be elucidated. The aim of this study was to investigate the regulatory roles of Circular RNAs (circRNAs) in SCI. The SCI mouse model and integrated bioinformatics analysis were used to identify the differentially expressed genes (DEGs). Functional enrichment analysis was conducted to study the related pathways. The circRNA-mediated ceRNA network and subnetwork was constructed based on circMir, TargetScan and miRanda. qRT-PCR, ELISA, flow cytometry, and luciferase reporter assays were carried out to validate the role of circ_0014637 (circ-Usp10) and microRNA(miR)-152-5p /CD84 in microglia. In all, 23 DE-circRNAs, 127 DE-miRNAs and 1327 DE-mRNAs were identified. We integrated these DEGs to construct a circRNA-miRNA-mRNA network. The circ-Usp10/miR-152-5p/CD84 axis was found to function in microglial activation. We also found that circ-Usp10 inhibited the secretion of proinflammatory factors in microglial BV2 cells. In addition, silencing circ-Usp10 significantly reduced the death of the neuronal cell line HT22. Taken together, we concluded that circ-Usp10 may function as a competing endogenous RNA (ceRNA) to promote microglial activation and induce neuronal death by targeting miR-152-5p/CD84. The circ-Usp10 may be a diagnostic biomarker and potential target for SCI therapy.

18.
Spectrochim Acta A Mol Biomol Spectrosc ; 267(Pt 1): 120564, 2021 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-34749112

RESUMO

A new method is presented for one to obtain the molar heat capacities of diatomic macroscopic gas with a full set of microscopic molecular rovibrational energies. Based on an accurate experimental vibrational energies subset of a diatomic electronic ground state, the full vibrational energies can be obtained by using the variational algebraic method (VAM), the potential energy curves (PECs) will be constructed by the Rydberg-Klein-Rees (RKR) method, the full set of rovibrational energies will be calculated by the LEVEL program, and then the partition functions and the molar heat capacities of macroscopic gas can be calculated with the help of the quantum statistical ensemble theory. Applying the method to the ground state HBr and HCl gases, it is found that the relative errors of the partition functions calculated in the temperature range of 300 âˆ¼ 6000 K are in excellent agreement with those obtained from TIPS database, and the calculated molar heat capacities are closer to the experimental values than those calculated by other methods without considering the energy levels of highly excited quantum states. The present method provides an effective new way for one to obtain the full set of molecular rovibrational energies and the molar heat capacities of macroscopic gas through the microscopic spectral information of a diatomic system.

19.
Artigo em Inglês | MEDLINE | ID: mdl-34599805

RESUMO

OBJECTIVES: This study aimed to characterize the systemic lipid profile of patients with asymptomatic hyperuricemia (HUA) and gout using lipidomics, and find potential underlying pathological mechanisms therefrom. METHODS: Sera were collected from Affiliated Hospital of Nanjing University of Chinese Medicine as center 1 (discovery and internal validation sets) and Suzhou Hospital of Traditional Chinese Medicine as center 2 (external validation set) including 88 normal subjects, 157 HUA and 183 gout patients. Lipidomics was performed by UHPLC-Q Exactive MS. Differential metabolites were identifed by both variable importance in the projection ≥ 1 in orthogonal partial least-squares discriminant analysis mode and false discovery rate adjusted p ≤ 0.05. Biomarkers were found by logistic regression and receiver operating characteristic (ROC) analysis. RESULTS: In the discovery set, a total of 245 and 150 metabolites respectively were found for normal subjects vs HUA and normal subjects vs gout. The disturbed metabolites included DAG, TAG, PC, PE, PI, etc. We also found 116 differential metabolites for HUA vs gout. Among them, the biomarker panel of TAG 18:1-20:0-22:1 and TAG 14:0-16:0-16:1 could differentiate well between HUA and gout. The area under ROC curve was 0.8288, the sensitivity was 82%, the specificity was 78% at a 95% confidence interval from 0.747-0.9106. In internal validation set, the predictive accuracy of TAG 18:1-20:0-22:1 and TAG 14:0-16:0-16:1 panel for differentiation of HUA and gout reached 74.38%, while 84.03% in external validation set. CONCLUSION: We identified serum biomarkers panel that have the potential to predict and diagnose HUA and gout patients.

20.
Opt Lett ; 46(19): 4932-4935, 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34598242

RESUMO

Theoretical resolution enhancement of confocal laser-scanning microscopy (CLSM) is sacrificed for the best compromise between optical sectioning and the signal-to-noise ratio (SNR). The pixel reassignment reconstruction algorithm can improve the effective spatial resolution of CLSM to its theoretical limit. However, current implementations are not versatile and are time-consuming or technically complex. Here we present a parameter-free post-processing strategy for laser-scanning microscopy based on deep learning, which enables a spatial resolution enhancement by a factor of ∼1.3, compared to conventional CLSM. To speed up the training process for experimental data, transfer learning, combined with a hybrid dataset consisting of simulated synthetic and experimental images, is employed. The overall resolution and SNR improvement, validated by quantitative evaluation metrics, allowed us to correctly infer the fine structures of real experimental images.

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