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1.
Sci Total Environ ; 750: 141233, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-32858286

RESUMO

Heavy metals pollutants are global concern due to their toxicities and persistence in the environment. Cd isotope signatures in soils and sediments change during weathering, and it remains unclear if Cd isotopes can effectively trace Cd sources in a riverine system. In this study, we investigate Cd concentration and its isotope compositions, as well as other heavy metals of sediments and related potential Cd sources in a riverine system. The results showed that the two river sediments evaluated were moderately polluted by Zn, Cr, and Cd, while the source samples (soil, sludge, waste, and raw materials) were seriously polluted by heavy metals derived from anthropogenic activities. According to comprehensive ecological risks, the two sediments have a moderate to low potential risk and more than half of all anthropogenic activities in the study area were at considerable or moderate potential risk. We determined that Cd pollution in river sediments was primarily derived from sewage treatment and outlets based on river flow direction and the isotope geochemical behaviors of the Cd isotope in nature conditions. This study further confirmed that analyzing Cd isotopes could be a powerful tool for tracing the source and destination of environmental Cd for multiple sources with similar Cd concentrations.

2.
Environ Sci Technol ; 54(22): 14674-14685, 2020 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-33147001

RESUMO

Understanding the recovery of anaerobic ammonium-oxidizing (anammox) bacteria after inhibition by dissolved oxygen (DO) is critical for the successful applications of anammox-based processes. Therefore, the effects of oxygen exposure (2 mg L-1 DO for 90 min) and subsequent recovery treatments [N2 purging or nano zero-valent iron (nZVI) addition] on the activity and gene expression in a Kuenenia stuttgartiensis enrichment culture were examined. Combining the self-organizing map clustering and enrichment analysis, we proposed the oxidative stress response of anammox bacteria based on the existing concepts of oxidative stress in microbes: the DO exposure triggered a stringent response in K. stuttgartiensis, which downregulated the transcription levels of genes involved in the central metabolism and diverted energy to a flagellar assembly and metal transport modules; these changes possibly promoted survival during the inhibition of anammox activity. According to the cotranscription with central catabolism genes, putative reactive oxygen species (ROS) scavenger genes (kat and sod) were presumed to detoxify the anammox intermediates rather than ROS. In addition, both activity and mRNA profiles with appropriate amount of nZVI addition (5 and 25 mg L-1) were close to that of control, which proved the effectiveness of nZVI addition in anammox recovery. These results would be relevant to the physio-biochemistry development of anammox bacteria and further enhancement of nitrogen removal in wastewater treatment.

3.
Jpn J Infect Dis ; 2020 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-33132301

RESUMO

Routine vaccination has proven to be highly effective in reducing the incidence of mumps. However, sporadic cases and/or mumps outbreaks do occur in children and adolescents younger than 15 years old, particularly among those 5-9 years old. To explore the characteristics of such outbreaks in Henan Province, clinical data of patients infected with MuV were collected, and the isolated MuV strains were phylogenetically analyzed. Of a total of 426 samples, MuV RNA targeting the small hydrophobic (SH) gene was detected in 153 samples. MuV-positive cases in each age group (< 5, 5-9,10-15, 16-19, and ≥ 20) accounted for 1%, 17%, 12%, 2% and 4% of the total number of cases, respectively. Phylogenetic analysis based on the SH gene sequences indicated that all of the isolated strains were of genotype F and isolates in the the same subcluster and identical SH gene sequences tended to be derived from the same community or municipalities when analyzed alongside epidemiological data. In conclusion, the incidence of mumps in Henan Province is high. The data provided in this study might promote further research in the clarification of specific cause of mumps outbreaks and which will facilitate the implementation of effective prevention and control measures.

4.
Cancer Invest ; : 1-24, 2020 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-33191800

RESUMO

Abnormal p53 immunohistochemical pattern (p53-AP, including overexpression, complete absence and heterogeneity) is surrogate of TP53 mutation. Using 742 cases of colorectal cancer (CRC), we show p53-AP is more common among type 2 diabetes (T2DM) patients than non-T2DM. Univariately, T2DM were significantly associated with p53-AP in overall patients, patients with microsatellite instability (MSI) stable/MSI-low phenotype or distal colorectal location. Furthermore, p53-AP was positively associated with lymph node metastasis and high TNM stage. Metformin treatment was negatively associated with p53-AP in T2DM patients. The results suggested T2DM might influence carcinogenesis, progression and prognosis via inducing TP53 mutation and abnormal p53 expression in CRC.

5.
Exp Neurol ; : 113524, 2020 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-33159930

RESUMO

Ubiquitin (Ub) C-terminal hydrolase L1 (UCHL1) is a multifunctional protein that is expressed in neurons throughout brain at high levels. UCHL1 deletion is associated with axonal degeneration, progressive sensory motor ataxia, and premature death in mice. UCHL1 has been hypothesized to play a role in the pathogenesis of neurodegenerative diseases and recovery after neuronal injury. UCHL1 hydrolyzes Ub from polyubiquitinated (poly-Ub) proteins, but also may ligate Ub to select neuronal proteins, and interact with cytoskeletal proteins. These and other mechanisms have been hypothesized to underlie UCHL1's role in neurodegeneration and response to brain injury. A UCHL1 knockin mouse containing a C90A mutation (C90A) devoid of hydrolase activity was constructed. The C90A mouse did not develop the sensory and motor deficits, degeneration of the gracile nucleus and tract, or premature death as seen in UCHL1 deficient mice. C90A and wild type (WT) mice were subjected to the controlled cortical impact (CCI) model of traumatic brain injury (TBI), and cell death, axonal injury and behavioral outcome were assessed. C90A mice exhibited decreased spared tissue volume, greater loss of CA1 hippocampal neurons and greater axonal injury as detected using anti-amyloid precursor protein (APP) antibody and anti- non-phosphorylated neurofilament H (SMI-32) antibody immunohistochemistry after CCI compared to WT controls. Poly-Ub proteins and Beclin-1 were elevated after CCI in C90A mice compared to WT controls. Vestibular motor deficits assessed using the beam balance test resolved by day 5 after CCI in WT mice but not in C90A mice. These results suggest that the hydrolase activity of UCHL1 does not account for the progressive neurodegeneration and premature death seen in mice that do not express full length UCHL1. The hydrolase activity of UCHL1 contributes to the function of the ubiquitin proteasome pathway (UPP), ameliorates activation of autophagy, and improves motor recovery after CCI. Thus, UCHL1 hydrolase activity plays an important role in acute injury response after TBI.

6.
Int Immunopharmacol ; : 107117, 2020 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-33162346

RESUMO

Bixin, an natural carotenoid extracted from the seeds of the Bixa orellana has been shown to possess numerous important pharmacological activities. The present study was aimed to investigate the mechanisms of Bixin on carbon tetrachloride (CCl4)-induced kidney inflammation, fibrosis and oxidative stress in mice. Our results showed that Bixin improved renal damage by decreasing the serum levels of creatinine, urea, uric acid and alleviating kidney fibrosis. Bixin ameliorated CCl4-induced inflammation in kidneys by reducing the levels of TNF-α and IL-1ß. Bixin suppressed oxidative stress by decreasing the MDA level and increasing the activation of SOD, CAT and GPx. Furthermore, Bixin increased the levels of PPAR-γ, NQO1, HO-1 and the nuclear translocation of Nrf2 in the kidneys of mice. Bixin supplementation inhibited the activation of TLR4, MyD88, NF-κB, TGF-ß and Smad3. Thus, this study demonstrated that Bixin possesses anti-oxidant, anti-inflammatory and anti-fibrosis properties through regulating the Nrf2/TLR4/MyD88 and PPAR-γ/TGF-ß1/Smad3 pathways.

7.
Int J Lab Hematol ; 2020 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-33226193

RESUMO

PURPOSE: Multiple myeloma (MM) is characterized by the malignant proliferation of plasma cells, which produce a monoclonal immunoglobulin protein. The role of 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC) has not yet been well studied in the area of MM. Thus, in the current study, we sought to examine the expression levels, including mRNA and protein levels of ATIC in MM. METHODS: Multiple myeloma microarray and RNA-seq data were screened from the SRA, GEO, ArrayExpress, and Oncomine databases. The mRNA level of ATIC was extracted from the high throughput data, and the prognostic value was studied. The protein level of ATIC was also detected by in-house immunohistochemistry on a tissue microarray. Potential signaling pathways were enriched with ATIC-related genes in MM. RESULTS: Both the mRNA and protein levels of ATIC were significantly upregulated in MM samples as compared to normal samples. Furthermore, the summarized Standardized Mean Difference was 1.66 with 674 cases of MM based on 10 independent studies including the in-house tissue microarray. The overall hazard ratio of ATIC in MM was 1.7 with 1631 cases of MM based on five microarrays. In the KEGG pathway analysis, the ATIC-related genes were mainly enriched in the pathway of complement and coagulation cascades. CONCLUSION: We provided the first evidence supporting the upregulation of ATIC may play an essential part in the tumorigenesis and development of MM. The promoting cancer capacity may be related to the pathway of complement and coagulation cascades.

8.
J Nanobiotechnology ; 18(1): 169, 2020 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-33208163

RESUMO

BACKGROUND: It is very important to develop a highly efficient cerebrospinal fluid (CSF) detection system with diagnosis and prediction function, for which the detection of circulating tumor cells (CTCs) in CSF is a good choice. In contrast to the past use of epithelial EpCAM as CTCs separation target, a cytoplasm protein of GFAP antibody was first selected to construct highly-sensitive immunomagnetic liposome beads (IMLs). The validation and efficiency of this system in capturing CTCs for brain tumors were measured both in vitro and in vivo. The associations between the numbers of CTCs in patients with their clinical characteristics were further analyzed. RESULTS: Our data show that CTCs can be successfully isolated from CSF and blood samples from 32 children with brain tumors. The numbers of CTCs in CSF were significantly higher than those in blood. The level of CTCs in CSF was related to the type and location of the tumor rather than its stage. The higher the CTCs number is, the more possibly the patient will suffer from poor prognosis. Genetic testing in GFAP CTC-DNA by sanger sequencing, q-PCR and NGS methods indicated that the isolated CTCs (GFAP+/EGFR+) are the related tumor cell. For example, the high expression of NPR3 gene in CSF CTCs was consistent with that of tumor tissue. CONCLUSIONS: The results indicated that GFAP-IML CTCs isolation system, combined with an EGFR immunofluorescence assay of antitumor marker, can serve as a brand-new method for the identification of CTCs for brain tumors. Via lumbar puncture, a minimally invasive procedure, this technique may play a significant role in the clinical diagnosis and drug evaluation of brain tumors.

9.
BMJ Open ; 10(11): e038832, 2020 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-33177137

RESUMO

INTRODUCTION: Studies addressing the development and/or validation of diagnostic and prognostic prediction models are abundant in most clinical domains. Systematic reviews have shown that the methodological and reporting quality of prediction model studies is suboptimal. Due to the increasing availability of larger, routinely collected and complex medical data, and the rising application of Artificial Intelligence (AI) or machine learning (ML) techniques, the number of prediction model studies is expected to increase even further. Prediction models developed using AI or ML techniques are often labelled as a 'black box' and little is known about their methodological and reporting quality. Therefore, this comprehensive systematic review aims to evaluate the reporting quality, the methodological conduct, and the risk of bias of prediction model studies that applied ML techniques for model development and/or validation. METHODS AND ANALYSIS: A search will be performed in PubMed to identify studies developing and/or validating prediction models using any ML methodology and across all medical fields. Studies will be included if they were published between January 2018 and December 2019, predict patient-related outcomes, use any study design or data source, and available in English. Screening of search results and data extraction from included articles will be performed by two independent reviewers. The primary outcomes of this systematic review are: (1) the adherence of ML-based prediction model studies to the Transparent Reporting of a multivariable prediction model for Individual Prognosis Or Diagnosis (TRIPOD), and (2) the risk of bias in such studies as assessed using the Prediction model Risk Of Bias ASsessment Tool (PROBAST). A narrative synthesis will be conducted for all included studies. Findings will be stratified by study type, medical field and prevalent ML methods, and will inform necessary extensions or updates of TRIPOD and PROBAST to better address prediction model studies that used AI or ML techniques. ETHICS AND DISSEMINATION: Ethical approval is not required for this study because only available published data will be analysed. Findings will be disseminated through peer-reviewed publications and scientific conferences. SYSTEMATIC REVIEW REGISTRATION: PROSPERO, CRD42019161764.

10.
Biomed Res Int ; 2020: 3049302, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33145344

RESUMO

The present study was to investigate the effect of mesenteric lymph duct drainage on lung inflammatory response, histological alteration, and endothelial cell apoptosis in septic rats. Animals were randomly assigned into four groups: control, sham surgery, sepsis, and sepsis plus mesenteric lymph drainage. We used the colon ascendens stent peritonitis (CASP) procedure to induce the septic model in rats, and mesenteric lymph drainage was performed with a polyethylene (PE) catheter inserted into mesenteric lymphatic. The animals were sacrificed at the end of CASP in 6 h. The mRNA expression levels of inflammatory mediators were measured by qPCR, and the histologic damage were evaluated by the pathological score method. It was found that mesenteric lymph drainage significantly reduced the expression of TNF-α, IL-1ß, and IL-6 mRNA in the lung. Pulmonary interstitial edema and infiltration of inflammatory cells were alleviated by mesenteric lymph drainage. Moreover, increased mRNA levels of TNF-α, IL-1ß, IL-6 mRNA, and apoptotic rate were observed in PMVECs treated with septic lymph. These results indicate that mesenteric lymph duct drainage significantly attenuated lung inflammatory injury by decreasing the expression of pivotal inflammatory mediators and inhibiting endothelial apoptosis to preserve the pulmonary barrier function in septic rats.

11.
Med Sci Monit ; 26: e926273, 2020 Oct 26.
Artigo em Inglês | MEDLINE | ID: mdl-33104528

RESUMO

BACKGROUND Bladder carcinoma (BLCA) is a leading cause of cancer-related deaths worldwide. The aim of this work was to develop an accurate stratification in predicting the prognosis and directing the treatment of BLCA patients based on small nucleolar RNAs (snoRNAs). MATERIAL AND METHODS Expression profiles of snoRNAs were downloaded from the SNORic database. The expression profiles and clinical outcomes of BLCA patients were analyzed. Survival-associated snoRNAs were identified and used to develop a novel risk score classifier. Genes in the whole genome that were significantly correlated with the included prognostic snoRNAs were used for functional enrichment analysis. RESULTS The results showed that age, American Joint Committee on Cancer (AJCC) stage, and tumor status were significantly correlated with overall survival (OS) of BLCA patients. We selected 12 survival-associated snoRNAs to build a prognostic signature. Patients were separated into high- and low-risk groups based on the median value of the risk score. Patients in the high-risk group and low-risk group have distinct clinical outcomes. The AJCC TNM stage showed moderate utility as a prognostic indicator for clinical outcome prediction. Then, clinical parameters and risk scores were entered in multivariate Cox analysis. Notably, the prognostic signature remained an independent significant prognostic risk factor. The pathway analysis suggested that these genes were enriched in several types of cancer and "Focal adhesion" pathways. CONCLUSIONS The prognostic signature defined by expression profiles of 12 survival-associated snoRNAs appears to be an excellent predictor of the clinical outcome of BLCA patients.

12.
Bioorg Chem ; 104: 104319, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33011531

RESUMO

The barks of Magnolia officinalis var. biloba, Magnoliae cortex, have been used as traditional Chinese medicines for several centuries. In this study, phytochemical investigation of M. officinalis var. biloba bark extract afforded five pairs of novel enantiomeric oligomeric neolignans, (±)-mooligomers A-E (1-5). (±)-1 and (±)-2 were two diastereomeric pairs of enantiomers with six C6-C3 subunits, and (±)-4 was a pair of previously unreported tetrameric neolignans bearing eight C6-C3 subunits. (±)-5 is the first example of a naturally occurring trilignan featuring an eight-membered ring with a magnolol moiety. The absolute configurations of (±)-1-(±)-5 were elucidated on the basis of HRESIMS, 1D and 2D NMR spectroscopy and electronic circular dichroism (ECD) calculations. Among the compounds tested for their PTP1B inhibitory activities, (±)-2, (±)-4 and (±)-5 displayed significant PTP1B inhibitory activities with IC50 values of 0.14-2.10 µM. Furthermore, a Molecular docking simulation of PTP1B and active compounds [(±)-2, (±)-4 and (±)-5] exhibited that these active compounds possess low binding affinities ranging from - 5.9 to - 7.7 kcal/mol.

13.
Water Res ; 187: 116454, 2020 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-33011570

RESUMO

Expansion in the size is an indispensable stage in the granular sludge life cycle, but little attention has been payed to the enlargement mechanism of granular sludge. Here, we propose a novel anammox granule enlargement mechanism by the self-assembly of heterogenous granules. Two different colors of anammox granules, dark-red granules (DR-Granules) and bright-red granules (BR-Granules) were found in an expanded granular sludge bed reactor. These two heterogenous granules were not isolated but were assembled into granules with a larger DR-Granule in the center and many smaller BR-Granules aggregated on the surface, increasing the overall granular size. Their physiochemical characteristics in terms of EPS, adherence, rheological properties, and microbial compositions, were identified and compared to elucidate the interaction between the different colors of granules. The BR-Granules created 92% more extracellular polymeric substances than the DR-Granules. This material blocked the passage of gas and substrate, leading to BR-Granules smaller size and a yield stress approximately 48% lower than that of the DR-Granules. Nevertheless, the BR-Granules had compact extracellular protein secondary structures and a high adherence rate to the surface of the DR-Granules, upon which they formed a compact adhered layer. These unique features enabled them to directionally adhere to DR-Granules in the core, that is, two heterogenous colors of granules self-assembled into large anammox granules. The enlargement mechanism was further supported by the abundance of K-strategy Ca. Kuenenia in the DR-Granules (inner layer) being higher than in the BR-Granules (outer layer; 2.9 ± 0.4% vs. 0.4 ± 0.1%; p = 0.0003) and by visualized confirmation that the larger BR-Granules wrapped around smaller DR-Granules inside. This demonstrates that heterogenous anammox granules actively self-assemble into large granules, which is an important step in the lifecycle of anammox granules.

14.
J Neurochem ; 2020 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-33113163

RESUMO

Schizophrenia is a severe psychiatric disorder with a strong hereditary component that affects approximately 1% of the world's population. The disease is most likely caused by the altered expression of a number of genes that function at the level of biological pathways or gene networks. Transcription factors (TF) are indispensable regulators of gene expression. EGR3 is a TF associated with schizophrenia. In the current study, DNA microarray and ingenuity pathway analyses (IPA) demonstrated that EGR3 regulates Reelin signaling pathway in SH-SY5Y cells. ChIP and luciferase reporter studies confirmed that EGR3 directly binds to the promoter region of RELN thereby activating RELN expression. The expression of both EGR3 and RELN was decreased during neuronal differentiation induced by retinoic acid (RA) in SH-SY5Y cells, and EGR3 over-expression reduced neurite outgrowth which could be partially reversed by the knockdown of RELN. The expression levels of EGR3 and RELN in peripheral blood of subjects with schizophrenia were found to be down-regulated (compared with healthy controls), and were positively correlated. Furthermore, data mining from public databases revealed that the expression levels of EGR3 and RELN were presented a positive correlation in post-mortem brain tissue of subjects with schizophrenia. Taken together, this study suggests that EGR3 is a novel TF of the RELN gene and regulates neurite outgrowth via the Reelin signaling pathway. Our findings contribute to the understanding of the regulatory role of EGR3 in the pathophysiology and molecular mechanisms of schizophrenia, and potentially to the development of new therapies and diagnostic biomarkers for the disorder.

15.
Neuropharmacology ; 181: 108350, 2020 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-33027625

RESUMO

Opioid addiction is a brain disease that severely harms society and personal health. Although the tremendous numbers of patients worldwide and emerged negative events, effective treatments for opioid addiction are still lacking. Neuropeptide Y (NPY) is one of the main orexigenic peptides that play vital roles in food intake and energy metabolism. However, increasing evidence indicates that NPY may have great potential in mediating reward effects and drug dependence. In the present study, we assessed the expression changes of NPY in the nucleus accumbens at different timepoints following morphine conditioned place preference (CPP) and investigated the functional importance of potential NPY changes. Our results showed that NPY expression significantly decreased in the nucleus accumbens shell (AcbSh) immediately after chronic morphine exposure. Subsequently, it increased rapidly at first and then gradually returned to normal levels. Further data indicated that these NPY changes were involved in morphine reward memory, demonstrated by a reduction in the extinction period after blocking of the Y5 receptor by L-152,804 in the AcbSh and a prolonged duration of the extinction period following the application of NPY. More importantly, the additional results revealed that L-152,804 also remarkably suppressed the reinstatement of morphine CPP. Together, our results indicate that a complicated plasticity of the NPY pathway in AcbSh occurs following morphine CPP, and this plasticity plays an important role in modulating morphine reward memory. These findings may enhance our understanding of the role of the NPY system in opioid addiction and indicate a promising target for opioid addiction treatment.

16.
Water Res ; 188: 116554, 2020 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-33128978

RESUMO

Novel control strategies for membrane biofouling with eco-friendly photocatalytic technology are critically needed in practical operation of membrane bioreactors (MBRs). In this study, a metal-organic frameworks (MOF) based photocatalytic membrane was firstly applied in an anammox MBR for a long-term biofouling control, where bacteria were inactivated and foulants were degraded simultaneously, with environmentally friendly and renewable visible light energy. By physicochemical characterization, the synthesized photocatalyst of CdS/MIL-101 showed superior visible-light photocatalytic ability, and the 1 wt% CdS/MIL-101 modified membrane C2 showed enhanced hydrophilicity and water permeability compared with the pristine membrane C0. In the long-term operation of anammox MBRs under waterproof lights irradiation, the filtration cycles of C2 (25-26 d) were obviously extended compared with C0 (10-14 d), while their average total nitrogen removal efficiencies were comparable up to 84%, indicating an excellent biofouling alleviation effect by using C2 with a satisfactory nitrogen removal performance maintained. By analysis of the biofilm on the fouled membranes, the organic foulants (especially extracellular polymeric substances) were degraded, and the live bacteria were inactivated effectively by the photocatalytic reactions of CdS/MIL-101 on C2. In the antimicrobial tests against model bacteria, C2 exhibited remarkable antimicrobial effect against both Gram-negative and Gram-positive bacteria with visible light irradiation by destruction of cell integrity with the inhibition rate of 92% for Escherichia coli and 95% for Staphylococcus aureus, respectively. In the model foulants (bovine serum albumin, sodium alginate, and humic acid) filtration tests, C2 showed higher antifouling capabilities, lower flux declining rates, and higher foulants rejection rates under visible light irradiation compared with C0. The reactive species of ·OH, e- and h+ generated on C2 were verified to play the predominant role in the anti-biofouling processes by simultaneous bacteria inactivation and foulants degradation. The findings offer a novel insight into the biofouling controlling in MBRs by simultaneous bacteria inactivation and foulants degradation with an eco-friendly method.

17.
Am J Sports Med ; 48(13): 3316-3321, 2020 11.
Artigo em Inglês | MEDLINE | ID: mdl-33044836

RESUMO

BACKGROUND: The all-arthroscopic anatomic glenoid reconstruction technique using a distal tibial allograft avoids damage to the subscapularis muscle and allows repair of the capsulolabral tissue. PURPOSE: To analyze the clinicoradiologic outcomes of patients who underwent this procedure to treat anterior shoulder instability with glenoid bone loss with a minimum 2-year follow-up. STUDY DESIGN: Case series; Level of evidence, 4. METHODS: Over 6 years, 73 patients (52 male and 21 female; mean age, 28.8 years) under the care of the same surgeon underwent arthroscopic stabilization with capsulolabral Bankart repair and bony allograft augmentation of the glenoid for recurrent shoulder instability with significant bone loss. Pre- and postoperative patient-reported functional assessment was performed using 2 questionnaires, the Western Ontario Shoulder Instability Index (WOSI) and the Disabilities of the Arm, Shoulder and Hand, and radiological assessment was performed using radiographs and computed tomography scans obtained preoperatively and approximately 1 year later (mean ± SD, 0.9 ± 1.1 years). RESULTS: The mean follow-up was 4.7 ± 1.1 years. The mean pre- and postoperative WOSI scores were 71.1 ± 17.5 and 25.6 ± 21.9, respectively (P < .001). There were no recurrences of dislocation, although 1 patient had symptoms of subluxation; however, 5 patients had hardware complications that required screw removal. There were no cases of nerve injury. Postoperative computed tomography scans were available for 66 patients. Seven patients were lost to follow-up. The graft union rate was 100%. Overall, graft resorption was <50% in 86% of patients (57/66). Eighteen patients (27%) had no resorption (grade 0), 39 (59%) had <50% (grades 1 and 2), and 9 (14%) had ≥50% (grade 3); however, none had symptoms of instability. The mean alpha angle of the screw between the screw shaft axis and the native glenoid axis was 18.3°± 5.7°. Graft positioning was flush with the glenoid in 61 of 66 patients (92.4%), and vertical positioning was excellent in 64 of 66 patients (97.0%) (3- to 5-o'clock position). CONCLUSION: Arthroscopic stabilization using distal tibial allograft augmentation resulted in excellent clinicoradiologic outcomes at a 2-year follow-up. This procedure has the advantages of being an anatomic reconstruction that addresses bony and soft tissue instability. However, long-term follow-up studies are necessary for better assessment of outcomes.

18.
Comput Biol Chem ; 89: 107383, 2020 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-33032037

RESUMO

RUNX family transcription factor 2 (RUNX2) overexpression has been found in various human malignancies. However, the expression levels of RUNX2 mRNA and protein in lung adenocarcinoma (LUAD) were not investigated. This study aims to thoroughly analysis the expression level and potential mechanisms of RUNX2 mRNA in LUAD. We applied in-house immunohistochemistry, high-throughput RNA-sequencing, and gene microarrays to comprehensively investigate the expression level of RUNX2 in LUAD. A pool standard mean difference (SMD) and summary receiver operating characteristic curves (SROC) were calculated to assess the integrated expression value of RUNX2 in LUAD. The hazard ratios (HRs) were integrated to evaluate the overall prognostic effect of RUNX2 on the LUAD patients. The differentially expressed genes (DEGs) of LUAD, the potential target genes of RUNX2, and its co-expressed genes were overlapped to obtain a set of specific genes for GO and KEGG enrichment analyses. RUNX2 overexpression in LUAD was validated using a large number of cases (2 418 LUAD and 1 574 non-tumor lung samples). The pooled SMD was 0.85 (95 % CI: 0.64-1.05) and the area under the curve (AUC) of the SROC was 0.86 (95 %CI: 0.83-0.89). The integrated HR was 1.20 [1.04-1.38], indicating that increased expression of RUNX2 was an independent risk factor for the poor survival of the LUAD patients. RUNX2 and its transcriptionally regulates potential target genes may promote cell proliferation and drug resistance of LUAD by modulating the cell cycle and MAPK signaling pathways. RUNX2 can provide new research directions for targeted drug therapy and drug resistance for LUAD treatment.

19.
Artigo em Inglês | MEDLINE | ID: mdl-33106903

RESUMO

Inland freshwater lakes have been widely considered as significant sources of CO2 to the atmosphere. However, long-term measurements of CO2 dynamics in lakes are still lacking, but are necessary due to their large temporal variations. Herein, we present the long-term dynamics of water parameters in Lake Donghu from 2002 to 2016, and further calculate the partial pressure of CO2 (pCO2) based on the measurements of pH, water temperature, and alkalinity from 2008 to 2016. The results revealed that a significantly high pCO2 occurred during the winter in Lake Donghu (p < 0.01), whereas no significant spatial difference was observed (p = 0.37). Statistical analysis indicated that the pCO2 in the lake was only positively correlated with the total phosphorus (TP) concentration (p < 0.05). A multilinear regression model provided the best predictors for the pCO2; however, it only explained 16% of the observed pCO2 variability. This indicates the complex factors that influenced the pCO2 in Lake Donghu between 2008 and 2016. Our estimated CO2 flux revealed that Lake Donghu acted as a small CO2 source to the atmosphere during this period, with a mean CO2 flux of 10.8 ± 37.4 mg m-2 day-1 corresponding to a mean CO2 emission of 0.13 ± 0.43 Gg year-1. The CO2 emission fluxes in Lake Donghu were much lower than the mean CO2 fluxes reported for other lakes in China and globally. Furthermore, the long-term evolution of the CO2 flux indicated that Lake Donghu has shifted between acting as a CO2 source and sink, which highlights the need for long-term monitoring to accurately evaluate CO2 emissions from lakes.

20.
Cell Transplant ; 29: 963689720968495, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33086893

RESUMO

Osteoarthritis (OA) is a major cause of disability worldwide with increasing age. Knee OA (KOA) is the most prevalent type of OA. Recently, it is considered that KOA is a whole joint disease, including articular cartilage, subchondral bone, synovium, ligaments, joint capsules, and muscles around the joint. Exosomes in knee joint are mainly secreted by articular chondrocytes and synoviocytes. They participate in cell and tissue cross-talk by carrying a complex cargo of proteins, lipids, nucleic acids, etc. Under normal conditions, exosomes maintain the microenvironmental homeostasis of the joint cavity. Under pathological conditions, the composition and function of exosomes changes, which in turn, disrupts the balance of anabolism and catabolism of articular chondrocyte and facilitates inflammatory responses, thus accelerating KOA progression. As a regenerative medicine, mesenchymal stem cells (MSCs) are promised to facilitate repair of degenerated cartilage and decelerate OA process. The therapeutic function of MSC mainly depends on MSC-derived exosomes, which can restore the homeostasis of the articular microenvironment. In the future, the specific mechanism of exosomes for OA treatment needs further elucidation, and the treatment effect of exosomes for long-term and/or severe OA needs further exploration.

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