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1.
J Agric Food Chem ; 70(18): 5570-5578, 2022 May 11.
Artigo em Inglês | MEDLINE | ID: mdl-35499918

RESUMO

Antimicrobial compounds from the commensal gut microbiota have gained much attention due to their multifunctionality in maintaining good health in the host and killing multidrug-resistant bacteria. Our previous study showed that Paenibacillus jilinensis YPG26 isolated from chicken intestine can antagonize multiple pathogens. Herein, we characterized a bacteriocin-like inhibitory substance, jileicin, purified from P. jilinensis YPG26. Mass spectrometry analysis revealed that jileicin was a protein consisting of 211 amino acids, which showed 88.98% identity to the SIMPL domain-containing protein. The jileicin showed a relatively broad-spectrum antibacterial ability, especially against enterococci. Additionally, the jileicin exhibited good stability after various treatments, no detectable resistance, no significant cytotoxicity, and very low levels of hemolytic activity. The mode of action against Enterococcus faecium demonstrated that jileicin could destroy cell membrane integrity, increase cell membrane permeability, and eventually lead to cell death. Furthermore, jileicin was efficient in controlling the growth of E. faecium in milk. In conclusion, jileicin, as a newly identified antibacterial agent, is expected to be a promising candidate for application in the food, pharmaceutical, and biomedical industries.


Assuntos
Bacteriocinas , Enterococcus faecium , Paenibacillus , Antibacterianos/metabolismo , Enterococcus , Enterococcus faecium/metabolismo , Paenibacillus/genética
2.
Poult Sci ; 101(7): 101888, 2022 Apr 05.
Artigo em Inglês | MEDLINE | ID: mdl-35550999

RESUMO

Since 2017, outbreaks of gosling astroviruses (GoAstV) causing the major symptoms related to gout in geese have posed a threat to China's poultry industry and caused huge economic losses. In this study, tissue samples from goslings with gout and urate deposition as the main symptoms were taken from 14 goose farms in different regions of China and screened for pathogen infection. The infection rate of GoAstV was 100%, whereas the infection rates of goose parvovirus, reovirus, Tembusu virus, and goose hemorrhagic polyomavirus were 2, 4, 0, and 0%, respectively. In total, 14 GoAstV strains were isolated and their complete genomes were sequenced. Based on the phylogenetic trees, the 14 isolated strains were classified as GoAstV (G-I) and were considered distant from strains belonging to GoAstV (G-II). The multiple sequence alignments indicated a tremendous amount of amino acid mutations in some parts of the encoding proteins of these strains; the main mutations were located in open reading frames (ORFs)-ORF1a and ORF2, such as M533V and F568S in ORF1a and A614T in ORF2. On the other hand, Further, 2 of the 14 GoAstV strains were possibly derived through inter-GoAstV-I recombination. Taken together, these findings indicate that GoAstVs are evolving in a more complex manner and have diverse transmission routes.

3.
J Healthc Eng ; 2022: 2718785, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35494521

RESUMO

Objective: To determine the effect of aqua astricta flushing on ocular pain after Trans-PRK. Method: Three hundred and seventy eyes from 185 myopic patients were prospectively recruited for the study. Patients underwent Trans-PRK in both eyes. Postsurgically, one eye from each patient was randomly assigned to the trial group, and refrigerated normal saline was used to rinse the eye. The contralateral eye was assigned to the control group, and room temperature normal saline was used to rinse the eye. The primary target was postoperative pain experienced at the end of surgery and on the first, second, and third days after surgery. Secondary targets were uncorrected distance visual acuity (UDVA), corrected distance visual acuity (CDVA), manifest refractive spherical equivalent (MRSE), and haze. Results: Patient pain scores gradually decreased over time, and the difference between time points of all patients was statistically significant (P < 0.05). Postoperative pain was not affected by patient cooperation, education level, refractive SE, optical zone, corneal bed, or cutting depth (P > 0.05). The level of pain at the end of surgery was affected by intraoperative rinsing. The pain level of the aqua astricta group was lower than the normal temperature saline group, and the difference was statistically significant (P < 0.01). Pain scores on the first, second, and third days after surgery were not affected by intraoperative rinse (P > 0.05). Conclusion: Trans-PRK is an important means of corneal refractive surgery, but postoperative pain remains unavoidable. These findings suggest that the use of cooled fluid during surgery reduces postoperative pain at the end of surgery.


Assuntos
Miopia , Ceratectomia Fotorrefrativa , Humanos , Miopia/cirurgia , Dor Pós-Operatória/prevenção & controle , Dor Pós-Operatória/cirurgia , Estudos Prospectivos , Solução Salina
4.
Microbes Infect ; : 104983, 2022 May 02.
Artigo em Inglês | MEDLINE | ID: mdl-35513233

RESUMO

Since 2011, the Gyrovirus galga 1 (GyVg1, previously recognized as avian gyrovirus 2) strain has extensively been detected worldwide. The virus has been identified in several species, including chickens, humans, domestic cats, and snakes, especially in China. Therefore, in this study, the presence of GyVg1 was investigated in various zoo animals to determine whether it exists in various species in Nanyang, China. A total of 63 whole blood samples (1 sample from each animal) from 24 animal species were collected from the Nanyang Zoo. Eight different GyVg1 strains were identified in eight types of animals using polymerase chain reaction, and the full genome of each strain was sequenced. The whole genome of four GyVg1 strains, namely, HN2019-H1, HN2019-T1, HN2019-SD1, and HN2019-L1 identified in hippopotamus (Hippopotamus amphibius,), tiger (Panthera tigris), sika deer (Cervus nippon), and lion (Panthera leo), respectively, comprised 2375 nucleotides (nt). The whole genome of the other strains, namely, HN2019-E1, HN2019-S1, HN2019-PF1, and HN2019-P1 identified in egret (Egretta garzetta), silver pheasant (Lophura nycthemera), peafowl (Pavonini), and common pheasants (Phasianus colchicus), respectively, comprised 2376 nt. Subsequently, a phylogenetic tree was constructed based on the 8 whole-genome sequence strains and 29 reference strains. These 37 strains were grouped into two major branches, group A and group B, and the 8 strains identified in this study were placed in group A. An analysis of the amino acids encoded by three open reading frames revealed some mutation sites unique to these eight strains. The substitution occurred at site 110 of viral protein 2 of HN2019-PF1, which is located in the highly conserved phosphatase motif WX7HX3CXCX5H (95-115aa). Recombination analysis revealed that, all these viral sequences were obtained as a result of recombination among the three GyVg1 strains (JL1511 and GS1512 from chickens and 17CC0810 from cat) from China and two strains (G17 from ferret of Hungary and RS-BR-15-2S from chicken of Brazil) from other countries. These findings indicate the complex evolution of GyVg1. Nevertheless, its transmission across the hosts is worth exploring.

5.
Front Cardiovasc Med ; 9: 840715, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35571198

RESUMO

Acute ST elevation myocardial infarction (STEMI) is a common acute and critical disease that requires rapid treatment within a limited window of time. In this study, we attempt to introduce a clinical pathway for the whole-process management of emergency STEMI based on the creation of a specific information system that matches the characteristics of emergency clinical work and evaluates their clinical value by quality control analysis. We deployed this system for 3 years and found that complications, heart failure, and medical costs during hospitalization were significantly reduced (p = 0.019) in patients with STEMI. By analyzing each link in the clinical pathway, our research indicates the clear clinical importance of developing methods to continuously improve data quality. Collectively, out research led to the optimization of an information system that will facilitate the clinical management of patients with STEMI.

6.
Int J Med Robot ; : e2424, 2022 May 16.
Artigo em Inglês | MEDLINE | ID: mdl-35575982

RESUMO

BACKGROUD: Continuous circular capsulorhexis (CCC) is a delicate ophthalmic procedure which may benefit from robot technology. Measuring the behaviors (physiological tremor, operation force) of surgeons provides baseline data to develop assistive CCC robot. METHODS: A forceps with fiber bragg grating and inertial sensors is used to measure the surgeons' behaviors while experts/novices perform CCC on ex-vivo pig eyes, in-vivo rabbit eyes and ex-vivo human lens. RESULTS: In pig / rabbit tests, the RMS tremor amplitude is 35.26 / 59.04 µm (expert/novice, transverse), 13.3 / 20.55 µm (axial). The RMS voluntary force (VF) and involuntary force (IF) are 8.97 / 17.16 mN, and 0.66 /1.90 mN, respectively. In human lens test, the RMS tremor amplitude is 24.0 µm (transverse, expert only), 9.88 µm (axial). The RMS VF and RMS IF are 9.04 mN (expert only) and 0.17 mN, respectively. CONCLUSIONS: The expert surgeons have better precision and less operation force. This article is protected by copyright. All rights reserved.

7.
Korean J Radiol ; 2022 May 09.
Artigo em Inglês | MEDLINE | ID: mdl-35555884

RESUMO

Gadoxetate magnetic resonance imaging (MRI) is widely used in clinical practice for liver imaging. For optimal use, we must understand both its advantages and limitations. This article is the outcome of an online advisory board meeting and subsequent discussions by a multidisciplinary group of experts on liver diseases across the Asia-Pacific region, first held on September 28, 2020. Here, we review the technical considerations for the use of gadoxetate, its current role in the management of patients with hepatocellular carcinoma (HCC), and its relevance in consensus guidelines for HCC imaging diagnosis. In the latter part of this review, we examine recent evidence evaluating the impact of gadoxetate on clinical outcomes on a continuum from diagnosis to treatment decision-making and follow-up. In conclusion, we outline the potential future roles of gadoxetate MRI based on an evolving understanding of the clinical utility of this contrast agent in the management of patients at risk of, or with, HCC.

8.
Orthop Surg ; 14(5): 868-875, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35434964

RESUMO

OBJECTIVES: To analyze the causes of misdiagnosis and missed diagnosis in spinal osteoid osteoma, and to put forward solutions to improve diagnosis accuracy and treatment efficacy in patients. METHODS: We performed a retrospective cohort study on patients with spinal osteoid osteoma in Beijing Jishuitan Hospital from January 1983 to September 2019. All patients underwent surgery. The outcome measures were the extent of local pain, nocturnal pain, radicular symptoms of extremities after surgery, and reduction or disappearance of lesions on CT after surgery. RESULTS: Thirty-seven patients with spinal osteoid osteoma were recruited in the study. A total of 27% were female, and the mean (SD) age at diagnosis was 21.3 (8.7) years. A total of 87.0% of patients presented with nocturnal pain, and 94.7% of patients were responsive to NSAIDS treatment. The mean (SD) time from the initial onset of symptoms to the final diagnosis was 14.7 (12.5) months. Only four of 37 (10.8%) patients were correctly diagnosed with spinal osteoid osteoma on the first visit to the local hospital. CT is associated with a higher diagnosis rate than X-ray or MRI on the first visit. Surgical navigation was used in 88.9% of patients who underwent curettage resection, and in 10% of patients who underwent en bloc resection. A total of 37 of 37 patients (100%) reported relief of local pain and radicular symptoms of extremities after surgery, and no recurrence of tumors was found during follow-ups. CONCLUSIONS: Spinal CTs are recommended to be performed if osteoid osteoma is suspected based on clinical manifestation, including nocturnal pain and responsiveness to NSAIDS treatment, to avoid misdiagnosis and missed diagnosis of spinal osteoid osteoma.


Assuntos
Neoplasias Ósseas , Osteoma Osteoide , Neoplasias da Coluna Vertebral , Anti-Inflamatórios não Esteroides/uso terapêutico , Neoplasias Ósseas/diagnóstico , Neoplasias Ósseas/cirurgia , Erros de Diagnóstico , Feminino , Humanos , Masculino , Diagnóstico Ausente , Osteoma Osteoide/diagnóstico , Osteoma Osteoide/cirurgia , Dor , Estudos Retrospectivos , Neoplasias da Coluna Vertebral/diagnóstico por imagem , Neoplasias da Coluna Vertebral/cirurgia
9.
ACS Omega ; 7(13): 11200-11207, 2022 Apr 05.
Artigo em Inglês | MEDLINE | ID: mdl-35415336

RESUMO

Wax deposition is one of the core issues affecting flow assurance studies of crude oil pipelines, particularly with deep and ultradeep water conditions. Nanocomposite pour point depressants (NPPDs) provide a novel and effective strategy for inhibiting wax deposition and have recently attracted increasing research attention. Although recent advances have been made in understanding the performance and mechanism of NPPDs, the effect of flow pattern remains an open question. In this paper, deposition thicknesses of waxy oils with different flow patterns and NPPD dosages were obtained using a flow loop experimental device. It was found that the NPPD used in the current work can effectively inhibit the formation of wax deposition layers in different flow patterns. The Avrami model-focused beam reflectance measurement and polarizing microscope experiment method were used to characterize crystallization kinetics parameters and mesoscopic structure parameters of wax crystals. The consistency of results from Avrami equation fitting parameters, wax crystal morphology, and particle number supported the validity of crystallization kinetics analysis. The mechanisms of NPPD in different flow regimes were discussed. The inhibition of laminar and turbulent deposition layers by NPPD was attributed to the improvement of wax crystal morphology and the reduction of wax crystal number, respectively. This has important consequences for our understanding of the utilization and mechanism of nanocomposite pour point depressants.

11.
Comput Intell Neurosci ; 2022: 4509575, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35419040

RESUMO

With the rapid development of global economy, the industrial cluster has become the new trend of world economic development. So far, the industrial cluster mode with space as the main division has been formed. The cooperation of industrial clusters is dynamic. The industries in the cluster cooperate with each other for mutual benefit and win-win, occupying a place in the fierce market competition. In addition, the industrial cluster is also conducive to strengthen the international economic division of labor and points out the direction of regional industrial transfer. China is now in a critical period of economic development; the industrial cluster plays an important role in China's industrial transformation and economic development. At present, the most common regional development mode in China is industrial cluster. The emergence of industrial cluster accelerates the development of industrial regional economy and the balance of industrial layout. Industrial clusters occupy a key position in China's economic industry chain, and the development and change in industrial clusters will directly affect the development of the entire industrial chain. This study first simulates the evolution path of industrial cluster and then establishes the relevant data model. Finally, through repast simulation, it puts forward conclusions and suggestions according to the verification results. The construction of dynamic model can realize the simulation of industrial cluster theory. According to the simulation results, we can find that the ecosystem in different stages will produce different characteristics; the formation and evolution of industrial clusters are actually the epitome of market development. In this process, the government guidance and market regulation are needed to accelerate the formation of industrial cluster ecosystem and increase the scale of industrial cluster.


Assuntos
Indústria de Laticínios , Ecossistema , China , Desenvolvimento Econômico , Aprendizado de Máquina , Redes Neurais de Computação
12.
Redox Biol ; 52: 102304, 2022 06.
Artigo em Inglês | MEDLINE | ID: mdl-35413643

RESUMO

As essential regulators of mitochondrial quality control, mitochondrial dynamics and mitophagy play key roles in maintenance of metabolic health and cellular homeostasis. Here we show that knockdown of the membrane-inserted scaffolding and structural protein caveolin-1 (Cav-1) and expression of tyrosine 14 phospho-defective Cav-1 mutant (Y14F), as opposed to phospho-mimicking Y14D, altered mitochondrial morphology, and increased mitochondrial matrix mixing, mitochondrial fusion and fission dynamics as well as mitophagy in MDA-MB-231 triple negative breast cancer cells. Further, we found that interaction of Cav-1 with mitochondrial fusion/fission machinery Mitofusin 2 (Mfn2) and Dynamin related protein 1 (Drp1) was enhanced by Y14D mutant indicating Cav-1 Y14 phosphorylation prevented Mfn2 and Drp1 translocation to mitochondria. Moreover, limiting mitochondrial recruitment of Mfn2 diminished formation of the PINK1/Mfn2/Parkin complex required for initiation of mitophagy resulting in accumulation of damaged mitochondria and ROS (mtROS). Thus, these studies indicate that phospho-Cav-1 may be an important switch mechanism in cancer cell survival which could lead to novel strategies for complementing cancer therapies.


Assuntos
Caveolina 1 , Mitofagia , Caveolina 1/genética , Caveolina 1/metabolismo , Mitocôndrias/metabolismo , Dinâmica Mitocondrial/fisiologia , Proteínas Mitocondriais/genética , Proteínas Mitocondriais/metabolismo , Mitofagia/fisiologia , Espécies Reativas de Oxigênio/metabolismo
13.
Front Cell Dev Biol ; 10: 836880, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35399524

RESUMO

Stimulator of interferon genes (STING) is a cytosolic DNA sensor or directly recognizes bacterial cyclic dinucleotides, which is required for the detection of microbial infection. Extracellular traps (ETs) are known to be part of the antimicrobial defense system. However, the implication of STING in ETs formation during microbial infection remains unknown. Here, we showed that STING contributed to Staphylococcus aureus (S. aureus)-induced ETs formation through the ROS-ERK signaling. STING deficiency exhibited decreased cell-free DNA (cfDNA) level, reduced expression of citrullinated histone H3 (CitH3), and diminished DNA colocalization with CitH3 and myeloperoxidase (MPO). Interestingly, NADPH oxidase-derived reactive oxygen species (ROS) promoted ETs formation, accompanied by increased activation of extracellular signal-regulated kinase 1 and 2 (ERK1/2) in S. aureus-stimulated bone marrow-derived macrophages (BMDMs). Corresponding to less ROS production, decreased ERK1/2 activation was shown in STING-/- BMDMs after S. aureus infection. Importantly, inhibiting the ROS-ERK signal reduced the ETs formation and the differences disappeared between WT and STING-/- BMDMs after S. aureus infection. Moreover, STING-/- BMDMs exhibited significantly increased levels of extracellular bacteria compared to WT BMDMs regardless of phagocytosis. In addition, such differences disappeared after DNase I treatment. DNase I treatment also facilitated pathogen colonization without affecting the inflammatory cells infiltration and pro-inflammatory factors secretion following pulmonary S. aureus infection. Furthermore, STING-/- mice presented decreased levels of cfDNA and CitH3, along with increased bacterial colonization compared to WT mice. Altogether, these findings highlighted that STING promoted ETs formation via the ROS-ERK signal for host defense against S. aureus infection.

14.
Artigo em Inglês | MEDLINE | ID: mdl-35339998

RESUMO

BACKGROUND/AIMS: Vasopressin is a powerful stimulator of vascular calcification, augmenting osteogenic signaling in vascular smooth muscle cells (VSMCs) including up-regulation of the transcription factors core-binding factor α-1 (CBFA1), msh homeobox 2 (MSX2), and SRY-Box 9 (SOX9), as well as of tissue-nonspecific alkaline phosphatase (ALPL). Vasopressin-induced osteogenic signaling and calcification require the serum- & glucocorticoid-inducible kinase 1 (SGK1). Known effects of SGK1 include up-regulation of Na+/H+ exchanger 1 (NHE1). NHE1 further participates in the regulation of reactive oxygen species (ROS). NHE1 has been shown to participate in the orchestration of bone mineralization. The present study, thus, explored whether vasopressin modifies NHE1 expression and ROS generation, as well as whether pharmacological inhibition of NHE1 disrupts vasopressin-induced osteogenic signaling and calcification in VSMCs. METHODS: Human aortic smooth muscle cells (HAoSMCs) were treated with vasopressin in the absence or presence of SGK1 siRNA, SGK1 inhibitor GSK-650394, and NHE1 blocker cariporide. Transcript levels were determined by using qRT-PCR, protein abundance by western blotting, ROS generation with 2',7'-dichlorofluorescein diacetate (DCFDA) fluorescence, as well as ALP activity and calcium content by using colorimetric assays. RESULTS: Vasopressin significantly enhanced the NHE1 transcript and protein levels in HAoSMCs, effects significantly blunted by SGK1 inhibition with GSK-650394 or SGK1 siRNA. Vasopressin increased ROS accumulation, an effect significantly blocked by NHE1 inhibitor cariporide. Vasopressin further significantly increased osteogenic markers CBFA1, MSX2, SOX9, and ALPL transcript levels, as well as ALP activity and calcium content in HAoSMCs, all effects significantly blunted by SGK1 silencing or in the presence of GSK-650394 and cariporide. CONCLUSION: Vasopressin stimulates NHE1 expression and ROS generation, an effect dependent on SGK1 and required for vasopressin-induced stimulation of osteogenic signaling and calcification of VSMCs.

15.
Front Pediatr ; 10: 853517, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35299672

RESUMO

Congenital abdominal aortic aneurysm is a rare disease with unknown etiology, and the common symptoms are abdominal pulsatile mass and pain caused by aneurysm rupture. The disease has a high mortality rate and fewer reports of surgical treatment. Here, we present a case of an idiopathic congenital abdominal aortic aneurysm. A 4-year-old boy had an abdominal pulsatile mass, and computed tomography angiography revealed an isolated infrarenal abdominal aortic aneurysm. To prevent rupture of the aneurysm, we repaired the aneurysm with artificial graft transplantation. No genetic mutation of the known congenital aneurysmal diseases was found in the whole-exome sequencing of the patient and his parents. There was no graft obstruction, and the patient grew well 40 months after surgery. Open surgery is the best treatment for idiopathic congenital abdominal aortic aneurysms. Surgical details such as timing and graft selection need to be further explored.

17.
Eur J Pharmacol ; 923: 174912, 2022 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-35339476

RESUMO

Astragaloside Ⅳ (AS-Ⅳ) is one of the main active components extracted from Astragalus membranaceus that exerts an antiatherosclerotic effect. Our study explored the underlying anti-apoptotic effects and the mechanisms of action of AS-Ⅳ in oxidized low-density lipoprotein (oxLDL)-stimulated macrophages and in vulnerable plaques. The results showed that AS-Ⅳ lowered the oxLDL-induced lipid content and reversed the oxLDL-induced reduction in cell viability and elevation in lactate dehydrogenase (LDH) leakage and apoptosis in RAW264.7 macrophages, similar to the effects of 4-phenylbutyric acid (PBA, an ER stress inhibitor). In addition, consistent with the effect exerted by PBA, AS-Ⅳ inhibited oxLDL-triggered ER stress activation by decreasing the level of inositol-requiring enzyme1 phosphorylation and transcription factor 6 nuclear translocation and upregulating the protein and mRNA expression of glucose-regulated protein 78 (GPR78) and C/EBP homologous protein (CHOP). As expected, autophagy activation was induced by AS-IV, evidenced by increased expression of microtubule-associated protein 1 light chain 3-Ⅱ (LC3-Ⅱ), autophagy-related gene 5, and beclin-1 in macrophages. Furthermore, after pretreatment with 3-methyladenine and beclin-1 small interfering RNA, the inhibitory role played by AS-Ⅳ in oxLDL-induced ER stress-CHOP-mediated macrophage apoptosis was weakened, while its inhibitory effect was further enhanced by rapamycin pretreatment. Moreover, administration of AS-Ⅳ or rapamycin to Apoe-/- mice upregulated LC3-Ⅱ expression and collagen content but decreased CHOP expression, macrophage apoptosis, and lipid areas. Overall, by promoting autophagy, AS-Ⅳ effectively protects macrophages from oxLDL-induced apoptosis mediated by ER stress-CHOP, which may reinforce the stability of atherosclerotic plaques.


Assuntos
Estresse do Retículo Endoplasmático , Transdução de Sinais , Animais , Apoptose , Autofagia , Proteína Beclina-1/metabolismo , Lipoproteínas LDL/metabolismo , Lipoproteínas LDL/farmacologia , Macrófagos , Camundongos , Saponinas , Sirolimo/farmacologia , Fator de Transcrição CHOP/metabolismo , Triterpenos
18.
J Ethnopharmacol ; 292: 115218, 2022 Jun 28.
Artigo em Inglês | MEDLINE | ID: mdl-35337919

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Baihe Dihuang Decoction is a well-known traditional Chinese medicine prescription (Also known as Lilium Henryi Baker and Rehmannia Glutinosa Decoction, LBRD) composed of Lilium Henryi Baker bulb and raw juice from Rehmannia Glutinosa (Gaertn) DC with the curative efficacy of nourishing yin and clearing heat based on the Chinese herbal medicine theory. It has been used as routine medication in treating depression combined with conventional western medicine in China for years. AIM OF THE STUDY: LBRD can attenuates GABAergic deficits in the medial prefrontal cortex (mPFC) of depression. This study aimed to investigate the mechanism of antidepressive properties of LBRD in the prefrontal GABAergic interneuron subtypes, including parvalbumin (PV), somatostatin (SST), vasoactive intestinal peptide (VIP)-positive neuron. MATERIALS AND METHODS: In this project, chronic unpredicted mild stress paradigm was adopted to construct depression model. After treated with LBRD standard decoction and behaviors test, the level of GABA associated miRNA/mRNA and GABAergic subtype-specific markers were detected by qRT-PCR and Western blot. The lncRNAs/miRNAs/GABA regulatory axis was verified by luciferase reporter assay, RNA immunoprecipitation, RNA pull-down assay, and theses changes were measured in LBRD administration with the use of immunofluorescence staining and RNA-fluorescence in situ hybridization. RESULTS: In the current study, we found that LBRD exhibited high efficacy based on the results of behavioral tests. Meanwhile, LBRD also improved the reduced GABA levels in depression by increasing the expression of lncRNA Neat1 and Malat1, as well as decreasing miRNA-144-3p and miRNA-15b-5p. Moreover, the level of Sst mRNA and protein that were harvested from the mPFC tissues of depression group was significantly lower than those in the control mice. While, these changes can be reverted by LBRD standard decoction administration. Whereas, neither chronic stress nor treatment can change the level of PV and VIP mRNAs and protein expression. In the SST-positive neuron of mPFC tissues, treatment with LBRD standard decoction resulted in the elevation of Gad-67, VGAT, GAT-3 and a reduction of miRNA-144-3p expression. CONCLUSIONS: These findings suggested that LBRD antidepressant activities may be related to ameliorating the SST-positive neuron deficits via regulating the miRNA-144-3p mediated GABA synthesis and release.


Assuntos
Lilium , MicroRNAs , Rehmannia , Animais , Antidepressivos/química , Antidepressivos/farmacologia , Antidepressivos/uso terapêutico , Depressão/tratamento farmacológico , Hibridização in Situ Fluorescente , Interneurônios/metabolismo , Camundongos , MicroRNAs/genética , MicroRNAs/metabolismo , Córtex Pré-Frontal/metabolismo , RNA Mensageiro/metabolismo , Somatostatina , Peptídeo Intestinal Vasoativo/metabolismo , Ácido gama-Aminobutírico/metabolismo
19.
Zhongguo Dang Dai Er Ke Za Zhi ; 24(3): 279-284, 2022 Mar 15.
Artigo em Inglês, Chinês | MEDLINE | ID: mdl-35351258

RESUMO

OBJECTIVES: To evaluate the effect of fluid load on the prognosis of children with sepsis-associated acute kidney injury (AKI) undergoing continuous renal replacement therapy (CRRT). METHODS: A total of 121 children who underwent CRRT for sepsis-associated AKI from August 2018 to March 2021 were enrolled in the retrospective study. According to the fluid load from admission or disease progression to CRRT, they were divided into three groups: low fluid load (fluid load: <5%; n=35), high fluid load (fluid load: 5% - <10%; n=35), and fluid overload (fluid load: ≥10%; n=51). Baseline data and clinical biochemical data before CRRT were collected for comparison and analysis. The Kaplan-Meier survival curve analysis was used for comparison of 28-day survival between groups. The multivariate logistic regression model was used to identify the influencing factors for the prognosis of the children. RESULTS: The survival analysis showed that the fluid overload group had a significantly higher 28-day mortality rate than the low fluid load and high fluid load groups (P<0.05). The multivariate logistic regression analysis showed that an increase in fluid overload volume was a risk factor for increased 28-day mortality in the fluid overload group, while earlier initiation of CRRT was a protective factor (P<0.05). CONCLUSIONS: Fluid overload before CRRT may increase the mortality in children with sepsis-associated AKI, and CRRT should be performed for these children as early as possible.


Assuntos
Injúria Renal Aguda , Terapia de Substituição Renal Contínua , Sepse , Injúria Renal Aguda/etiologia , Injúria Renal Aguda/terapia , Criança , Humanos , Prognóstico , Estudos Retrospectivos , Sepse/complicações , Sepse/terapia
20.
Virulence ; 13(1): 471-482, 2022 12.
Artigo em Inglês | MEDLINE | ID: mdl-35254202

RESUMO

Extracellular trap (ET) appears as a double-edged sword for the host since it participates in host immune defense by entrapping pathogens, while excessive ET release also contributes to various diseases progression including atherosclerosis, cancer, and autoimmune disorders. A better understanding of ET formation and regulation will be beneficial for developing strategies for infection control and ET-associated disease treatment. There is some evidence indicating that prior infection can enhance extracellular killing. Neutrophils from cancer or sepsis are predisposed to generate ET. It is reasonable to suspect that ET may be trained to form as a memory response, just like cytokine memory response termed "trained immunity." The mice were intraperitoneally injected with heat-killed Candida albicans (HK-C. albicans), 3 days later bone marrow-derived macrophages (BMDM) were isolated and challenged with Clostridium perfringens as a second stimulation. We found that HK-C. albicans priming enhanced ET formation upon Clostridium perfringens infection, accompanied by increased extracellular killing capacity. Mannan priming also enhanced ET formation. Since ETs memory was induced in chicken PBMC, ETs memory may be evolutionarily conserved. Moreover, mTOR was required for ETs memory response. Collectively, this study showed that ETs can be trained as a memory response and indicated that memory property of ETs should be considered during the understanding of recurrent infection and ET-associated disorders.


Assuntos
Armadilhas Extracelulares , Animais , Candida albicans , Imunidade Inata , Leucócitos Mononucleares , Macrófagos , Camundongos , Neutrófilos
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