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1.
Dermatol Ther ; : e13690, 2020 May 29.
Artigo em Inglês | MEDLINE | ID: mdl-32468693

RESUMO

The aim of this study was to explore the main factors affecting the occurrence of dandruff in healthy people (nondisease-induced scalp desquamation). This study analyzed the fungal microbial diversity of the scalp in Chinese teenage volunteers and measured scalp sebum secretion, the scalp pH value, and scalp transepidermal water loss. The amount and size of dandruff were measured, and the main factors that influence dandruff in the normal population were identified using principal component analysis. The results showed that an increase in Malassezia restricta led to an increased amount of dandruff in the mild and moderate groups. Conversely, this was not found for individuals in the severe group, whose dandruff symptoms were influenced by scalp barrier function. In terms of dandruff area grouping, the pH value and the amount of sebum secretion were the main factors, with the barrier function and microbial diversity being secondary factors. Dandruff cosmetics should emphasize different treatments for different types of dandruff to achieve better antidandruff effects. The results of this study provide a new theoretical basis for the development of multiple targets for antidandruff agents aimed at the normal population.

2.
Dermatology ; 236(2): 160-169, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31553991

RESUMO

BACKGROUND: Atopic dermatitis (AD) is a chronic, recurrent skin condition with recently increased incidence in younger children. AD development has been correlated with the skin microbiome, and Staphylococcus aureus enrichment causes significant increases in skin lesions. OBJECTIVE: Our objectives were to compare the microbial diversity of the cheek skin of children with or without AD aged 0-1 years in China, and to determine whether 4 types of skin-isolated bacteria could inhibit S. aureus in vitro. METHODS: The skin microbial samples of cheek skin of children were sequenced by 16S rRNA V1-V2 region. Four skin isolated bacterial fermentation supernatants were tested for effects on S. aureus growth, membrane formation, and induction of cytokine secretion from HaCaT cells. RESULTS: Bacterial diversity decreased significantly in skin with severe AD compared to healthy skin (p < 0.01). Seven phyla had content >1%, 4 of which differed in AD (p < 0.05). 38 genera had content >1%, 15 differed (p < 0.05). Differences in 8 species were observed (p < 0.05). In vitro antibacterial and cellular experiments showed that S. aureus growth, biofilm formation, and induction of interleukin (IL)-1α and IL-6 secretion from HaCaT cells were significantly inhibited by Klebsiella oxytoca, Kocuria rhizophila, and Staphylococcus epidermidis culture supernatants (p < 0.05). CONCLUSION: Skin microbiome changes in children varied with age and with AD. There were complex interactions between skin isolated bacteria and S. aureus which could inhibit S. aureus growth and biofilm formation in vitro, suggesting that these microorganisms could be used in AD treatment.

3.
Exp Dermatol ; 28(11): 1289-1297, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31472099

RESUMO

BACKGROUND: The microbiological basis of diaper dermatitis has not been clearly elucidated; however, a better understanding of microbial colonization may be vital for developing appropriate therapies. METHODS: Using 16S-rRNA gene sequencing technology, we characterized and compared the bacterial communities obtained from the buttock skin sites of children with diaper dermatitis and from healthy controls. Bacterial diversity in the buttock lesion area and subsequent recovery after emollient treatment have been discussed herein. RESULTS: In buttock skin of children with or without diaper dermatitis, Staphylococcus and Anaerococcus were predominant in the total skin microbiome. Compared with the healthy group, the overall skin bacterial richness and diversity were higher in children with diaper dermatitis, with the abundance of Proteobacteria being significantly higher. In the diaper dermatitis group, the richness of Enterococcus, Erwinia and Pseudomonas was significantly higher, and the levels of Clostridium and Actinomyces were significantly lower than those in healthy children. Richness of Staphylococcus aureus was significantly higher in the diaper dermatitis group, whereas that of Staphylococcus epidermidis and Bifidobacterium longum was lower. Staphylococcus epidermidis and Staphylococcus haemolyticus, the dominant species found in buttock skin, were observed to recover earlier after the disease had improved through emollient treatment. CONCLUSION: Staphylococcus epidermidis, as skin probiotic bacterium, and B longum, Clostridium butyricum and Lactobacillus ruminis, which are intestinal probiotic bacteria, are significantly decreased in diaper dermatitis lesions. These changes in the buttock skin microflora indicate an imbalance in the microflora and suggest that the intestinal microflora may be undergoing dynamic changes. The results of this study suggest that probiotic bacterial supplementation may be useful in the treatment and prevention of diaper dermatitis.

4.
Eur J Clin Microbiol Infect Dis ; 38(9): 1677-1685, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31152265

RESUMO

Atopic dermatitis (AD), a chronic relapsing inflammatory pruritic skin disorder with a unique pathophysiology, has a high incidence in the perioral zone among infants. This study aimed to analyze the association of skin microfloral dynamics with disease severity and treatment of AD in 0-1-year-old infants. Based on the eczema area and severity index, subjects were divided into five groups, i.e., mild, moderate, severe, and severe post-treatment, with a healthy control group, and bacterial density at the perioral lesion, disease severity, and treatment were assessed in 0-1-year-old infants with AD. The perioral lesions were colonized predominantly by Firmicutes, followed in abundance by Proteobacteria, Actinobacteria, and Bacteroidetes. In the phylum Firmicutes, Streptococcus was the most predominant genus. In AD infants, the abundance of Bacteroidetes and Fusobacterium decreased significantly with an increase in disease severity (p < 0.01). The abundance of 6 genera, including Prevotella, decreased significantly with an increase in disease severity (p < 0.05). The abundance of Prevotella melaninogenica decreased gradually with an increase in disease severity and increased after treatment; this trend was reversed for Corynebacterium simulans. A reduction in the abundance of Staphylococcus and an increase in that of skin microflora including Prevotella spp., Staphylococcus epidermidis, and Erwinia dispersa were associated with treatment and clinical improvement. Skin bacterial composition varies with AD severity, and Corynebacterium simulans and Prevotella melaninogenica are positively and negatively correlated with AD severity, respectively. This study provides a theoretical basis to identify potential biomarkers AD occurrence and pathogenesis.


Assuntos
Bactérias/efeitos dos fármacos , Dermatite Atópica/microbiologia , Dermatite Perioral/microbiologia , Microbiota , Pele/microbiologia , Bactérias/classificação , Dermatite Atópica/tratamento farmacológico , Dermatite Perioral/tratamento farmacológico , Eczema , Humanos , Lactente , Recém-Nascido , RNA Ribossômico 16S , Índice de Gravidade de Doença
5.
J Zhejiang Univ Sci B ; 14(2): 97-105, 2013 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-23365008

RESUMO

Oat contains different components that possess antioxidant properties; no study to date has addressed the antioxidant effect of the extract of oat bran on the cellular level. Therefore, the present study focuses on the investigation of the protective effect of oat bran extract by enzymatic hydrolysates on human dermal fibroblast injury induced by hydrogen peroxide (H(2)O(2)). Kjeldahl determination, phenol-sulfuric acid method, and high-performance liquid chromatography (HPLC) analysis indicated that the enzymatic products of oat bran contain a protein amount of 71.93%, of which 97.43% are peptides with a molecular range from 438.56 to 1301.01 Da. Assays for 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity indicate that oat peptide-rich extract has a direct and concentration-dependent antioxidant activity. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) colorimetric assay and the TdT-mediated digoxigenin-dUTP nick-end labeling (TUNEL) assay for apoptosis showed that administration of H(2)O(2) in human dermal fibroblasts caused cell damage and apoptosis. Pre-incubation of human dermal fibroblasts with the Oatp for 24 h markedly inhibited human dermal fibroblast injury induced by H(2)O(2), but application oat peptides with H(2)O(2) at same time did not. Pre-treatment of human dermal fibroblasts with Oatp significantly reversed the H(2)O(2)-induced decrease of superoxide dismutase (SOD) and the inhibition of malondialdehyde (MDA). The results demonstrate that oat peptides possess antioxidant activity and are effective against H(2)O(2)-induced human dermal fibroblast injury by the enhanced activity of SOD and decrease in MDA level. Our results suggest that oat bran will have the potential to be further explored as an antioxidant functional food in the prevention of aging-related skin injury.


Assuntos
Avena/química , Fibroblastos/efeitos dos fármacos , Fibroblastos/fisiologia , Peróxido de Hidrogênio/farmacologia , Extratos Vegetais/farmacologia , Sementes/química , Fenômenos Fisiológicos da Pele/efeitos dos fármacos , Células Cultivadas , Criança , Citoproteção , Fibroblastos/patologia , Humanos , Masculino
6.
Life Sci ; 90(11-12): 424-31, 2012 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-22273755

RESUMO

AIMS: Recent studies have shown that dermal fibroblasts possess multiple types of voltage-dependent K(+) channels, and the activation of these channels induces apoptosis. In the present study, we aimed to investigate whether hydrogen peroxide (H(2)O(2)), an oxidative stress inducer, could modulate these channels or induce human dermal fibroblasts injury. MAIN METHODS: The effects of H(2)O(2) on K(+) currents were studied using a whole-cell recording. Intracellular PKC levels were measured with a direct human PKC enzyme immunoassay kit. Cell viability was assessed using PI staining and apoptotic nuclei were detected with TdT-mediated digoxigenin-dUTP nick-end labelling assay (TUNEL) assay. KEY FINDINGS: Treatment of cells with 100µM H(2)O(2) resulted in a partially reversible increase in non-inactivating outward K(+) currents and an alteration in the steady-state activation property of the channels. The H(2)O(2)-induced increase in K(+) currents was mimicked by a PKC activator, and was blocked by the PKC inhibitor or the large conductance Ca(2+)-activited K(+) (BK) channel blockers. The intracellular PKC levels were significantly enhanced by H(2)O(2) treatment in a concentration-dependent manner. After exposure to H(2)O(2), evaluation of fibroblasts survival rate and damaged cell number with TUNEL-positive nuclei revealed an increased cell injury. Blocking the K(+) channels with blockers significantly decreased the H(2)O(2)-induced human dermal fibroblasts injury. SIGNIFICANCE: Our results revealed that H(2)O(2) could enhance BK currents by PKC pathway. Increased K(+) currents might be related to H(2)O(2)-induced human dermal fibroblasts injury. The results reported here contribute to our understanding of the mechanism underlying H(2)O(2)-induced human dermal fibroblasts injury.


Assuntos
Derme/metabolismo , Fibroblastos/metabolismo , Peróxido de Hidrogênio/toxicidade , Canais de Potássio Cálcio-Ativados/metabolismo , Apoptose/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Derme/citologia , Humanos , Técnicas Imunoenzimáticas , Marcação In Situ das Extremidades Cortadas , Técnicas de Patch-Clamp , Proteína Quinase C/metabolismo
7.
Int J Dermatol ; 43(11): 801-7, 2004 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-15533060

RESUMO

Asiaticoside, isolated from Centella asiatica, promotes fibroblast proliferation and extracullar matrix synthesis in wound healing. The precise mechanism, however, in molecular and gene expression levels still remains partially understood. Using cDNA microarray technology, the alternation of genes expression profiles was determined in a human dermal fibroblast in vitro in the presence of asiaticoside (30 microg/ml). Fifty-four genes, with known functions for cell proliferation, cell-cycle progression and synthesis of the extracellular matrix, were significantly up-regulated in our "whole-genes nest" expression profile at various timepoints. Furthermore, mRNA levels and protein productions of certain genes responsible for extracellular matrix (ECM) synthesis (e.g. encoding type I and type III collagen proteins) were evaluated by Northern blot and radioimmunoassay, respectively. As a result, there is a close correlation among the gene profile, mRNA and protein production in the cells response to asiaticoside stimulation. This information could be used for exploring the target genes in response to asiaticoside in fibroblasts.


Assuntos
Ciclo Celular/efeitos dos fármacos , Ciclo Celular/fisiologia , Colágeno/biossíntese , Fibroblastos/efeitos dos fármacos , Fibroblastos/fisiologia , Triterpenos/farmacologia , Ciclo Celular/genética , Divisão Celular/efeitos dos fármacos , Células Cultivadas , Fibroblastos/citologia , Regulação da Expressão Gênica , Humanos , Pele/citologia
8.
Cell Motil Cytoskeleton ; 58(2): 127-36, 2004 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-15083534

RESUMO

Cell migration is mediated by ion channels and transporters, and plays crucial roles in a variety of physiological and pathological processes. Previously, our studies have shown that a Ca(2+)-regulated K(+) current exists in B-16 murine melanoma cells, and that endothelin-1 (ET-1) inhibits the K(+) current via a PKC-dependent pathway. In the present study, patch-clamp whole-cell recording and transwell migration assays were used to examine the effects of ET-1 on B-16 murine melanoma cell migration. ET-1 (100 nM in the injection pipette and 10 nM in the incubation medium) decreased the K(+) current amplitude by 33.0 +/- 2.5% and inhibited migration of B-16 cells by 57.4 +/- 9.4%. Similarly, the Ca(2+)-regulated K(+) channel blockers, BaCl(2) and quinidine, decreased the K(+) current by 20.5 +/- 1.0% and 36.6 +/- 1.2%, respectively, and slowed migration of B-16 melanoma cells by 37.1 +/- 8.6% and 42.7 +/- 8.8%, respectively. The effect of ET-1 on the K(+) current and cell migration was simulated by ET-3. In contrast, the K(+) channel opener, diclofenac, increased the K(+) current by 128.8 +/- 11.7%, 257.4 +/- 35.8% at concentrations of 1 and 5 mM, respectively. Likewise, the migration of B-16 murine melanoma cells dramatically increased by 75.6 +/- 12.7% in the presence of 100 microM diclofenac in incubation medium. Furthermore, the ET-1- and ET-3-induced inhibition of K(+) current and migration was abrogated by diclofenac. In the presence of diclofenac, ET-1 only reduced the K(+) current amplitude by 10.6 +/- 1.1%, and slowed B-16 cell migration by only 10.8 +/- 8.9%. The results suggest that the K(+) channel-dependent migration of B-16 melanoma cells is modulated by ET-1. Cell Motil.


Assuntos
Movimento Celular/fisiologia , Endotelina-1/metabolismo , Melanoma Experimental/metabolismo , Potássio/metabolismo , Animais , Movimento Celular/efeitos dos fármacos , Diclofenaco/farmacologia , Endotelina-1/farmacologia , Melanoma Experimental/patologia , Camundongos , Potássio/antagonistas & inibidores , Canais de Potássio/efeitos dos fármacos
9.
Pigment Cell Res ; 16(5): 463-9, 2003 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-12950721

RESUMO

It is well established that endothelin-1 (ET-1) plays a role in differentiation and proliferation in a variety of cells such as fibroblasts and human melanoma cells via a receptor-mediated mechanism. However, whether ET-1 modulates ion channel activity in these cell types is still unknown. In this report, we recorded the voltage-dependent outward K+ current in cultured B16 melanoma cells using the patch-clamp technique. Biophysical and pharmacological properties of the K+ current, and the effect of ET-1 on the K+ current were investigated. When cells were loaded with a Ca(2+)-chelating agent (EGTA or BAPTA), the K+ current amplitude gradually increased with time after establishment of the whole cell configuration. Replacement of Ca2+ with Co2+ in the extracellular medium caused no significant modulation of the K+ current amplitude. Addition of BaCl2 or quinidine to the extracellular solution reduced the K+ current amplitude, whereas the K+ current was insensitive to tetraethylammonium. ET-1 (10 nM) reversibly decreased the K+ current amplitude and accelerated the decay of the K+ current. The ET-1-induced inhibitory effect displayed no desensitization following repeated ET-1 application. Pretreatment with pertussis toxin (PTX) or perfusion of cells with the protein kinase C (PKC) inhibitor H-7 abolished the inhibitory effect of ET-1 on the K+ current. We conclude that the outward K+ current recorded in murine B-16 melanoma cells represents a Ca(2+)-inactivated K+ current, and that the inhibitory effect of ET-1 on the K+ current may reveal a novel mechanism to control the differentiation and proliferation of melanoma cells.


Assuntos
Endotelina-1/fisiologia , Melanoma Experimental/metabolismo , Canais de Potássio Cálcio-Ativados/fisiologia , 1-(5-Isoquinolinasulfonil)-2-Metilpiperazina/farmacologia , Animais , Cálcio/fisiologia , Linhagem Celular Tumoral , Cobalto , Ácido Egtázico/farmacologia , Endotelina-1/antagonistas & inibidores , Humanos , Técnicas de Patch-Clamp , Toxina Pertussis/farmacologia , Bloqueadores dos Canais de Potássio/farmacologia , Canais de Potássio Cálcio-Ativados/metabolismo , Proteína Quinase C/metabolismo
10.
Artigo em Chinês | MEDLINE | ID: mdl-14761521

RESUMO

OBJECTIVE: To study the potential aging effect on workers exposed to acrylonitrile (ACN). METHODS: The deletion rates of mitochondrial DNA (mtDNA) in peripheral blood nucleate cells of 47 exposed workers and 47 non-exposed workers (as control), as well as 12 old people and 12 young people were measured with polymerase chain reaction (PCR). RESULTS: The positive rates of mtDNA deletion in peripheral blood nucleate cells were 17.02% in the workers exposed to ACN and 25.00% in group of old people. However, the mtDNA deletion was not detected in the control group and young people. CONCLUSIONS: ACN could induce mtDNA deletion in peripheral blood nucleate cells of the exposed workers. There may be a potential molecular effect of occupational ACN exposure on workers' aging.


Assuntos
Acrilonitrila/toxicidade , Envelhecimento/efeitos dos fármacos , Células Sanguíneas/efeitos dos fármacos , Dano ao DNA , DNA Mitocondrial/efeitos dos fármacos , Adolescente , Idoso , Idoso de 80 Anos ou mais , Células Sanguíneas/ultraestrutura , Humanos , Exposição Ocupacional
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