Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 11 de 11
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Immunol Lett ; 229: 8-17, 2020 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-33186635

RESUMO

In abnormal glycosylation, molecules of glucose or other carbohydrates in living organisms are inappropriately attached to proteins, which causes protein denaturation. Abnormal glycosylation modification is known to directly or indirectly affect the tumor escape process, but very few studies have been performed on whether protein glycosylation changes the structure and function of immune cells and immune molecules and thereby regulates the occurrence and development of tumor escape. Therefore, this article summarizes the effect of the immune system on tumor escape in association with the abnormal glycosylation process from an immunological perspective.

2.
Front Pharmacol ; 11: 556885, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33013395

RESUMO

Novel coronavirus (COVID-19) pneumonia has become a major threat to worldwide public health, having rapidly spread to more than 180 countries and infecting over 1.6 billion people. Fever, cough, and fatigue are the most common initial symptoms of COVID-19, while some patients experience diarrhea rather than fever in the early stage. Many herbal medicine and Chinese patent medicine can significantly improve these symptoms, cure the patients experiencing a mild 22form of the illness, reduce the rate of transition from mild to severe disease, and reduce mortality. Therefore, this paper summarizes the physiopathological mechanisms of fever, cough, fatigue and diarrhea, and introduces Chinese herbal medicines (Ephedrae Herba, Gypsum Fibrosum, Glycyrrhizae Radix et Rhizoma, Asteris Radix et Rhizoma, Ginseng Radix et Rhizoma, Codonopsis Radix, Atractylodis Rhizoma, etc.) and Chinese patent medicines (Shuang-huang-lian, Ma-xing-gan-shi-tang, etc.) with their corresponding therapeutic effects. Emphasis was placed on their material basis, mechanism of action, and clinical research. Most of these medicines possess the pharmacological activities of anti-inflammatory, antioxidant, antiviral, and immunity-enhancement, and may be promising medicines for the treatment or adjuvant treatment of COVID-19 patients.

3.
Zhongguo Zhong Yao Za Zhi ; 45(15): 3608-3616, 2020 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-32893550

RESUMO

Fatigue is a widespread and complex physiological phenomenon. Chronic fatigue can lead to cardiovascular dysfunction, mental disorders and other serious pathological reactions. Therefore, how to relieve fatigue accurately and effectively is an important proposition to implement the concept of "Healthy China" in the new era. As an important part of Chinese medicine health industry, Chinese medicine health food has been developing rapidly in recent years. At present, there are 1 157 kinds of anti-fatigue health food on the market in China, most of which are single Chinese medicine and its compound. However, their functions are generally labeled as "anti-fatigue", and their function positioning is too extensive and unclear. With the deepened understanding of fatigue classification and its physiological and pathological basis, it is urgent to be combined with the progress of modern chemical and pharmacological stu-dies to differentiate and precisely position the anti-fatigue health effects of traditional Chinese medicine. For this purpose, the classifications of fatigue were summarized in this paper, and the mechanism of fatigue was explained from the aspects of energy metabolism, accumulation of metabolites, oxidative stress, inflammation, hypothalamus pituitary adrenal axis and so on. We selected 10 traditional Chinese medicines which are most frequently used in health food, analyzed their anti-fatigue effect mechanisms, and summarized the best types of anti-fatigue food, so as to promote the scientific development of anti-fatigue health food industry, expand the market application scope of anti-fatigue health food, better respond to the construction of a healthy China and serve for people's health.


Assuntos
Medicamentos de Ervas Chinesas , Plantas Medicinais , China , Alimentos , Medicina Tradicional Chinesa
4.
ACS Appl Mater Interfaces ; 12(36): 40094-40107, 2020 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-32805811

RESUMO

Although a plethora of nonviral gene vectors have been developed for potential gene therapy, imageable gemini surfactants with stimuli-responsiveness and high transfection efficiency are still scarce for gene delivery. Herein, three gemini amphiphiles (DEDPP-4/8/12) consisting of an aggregation-induced emission (AIE) central fluorophore: 5,6-diphenylpyrazine-2,3-diester (DEDPP), decorated with triazole-[12]aneN3 as the hydrophilic moiety and alkyl chains of various lengths as the hydrophobic moiety, were designed and synthesized for trackable gene delivery via optical imaging. All three amphiphiles exhibited ultralow critical micelle concentrations (CMCs) (up to 3.40 × 10-6 M), prominent two-photon absorption properties, and solvatochromic fluorescence. Gel electrophoresis assays demonstrated that the migration of plasmid DNA was completely retarded after condensation with these gemini amphiphiles at low concentrations (up to 10 µM). In addition, the ester bond in these amphiphiles may facilitate vector degradation and DNA release, in response to esterase and the acidic environment inside cells. Upon self-assembly with DOPE to form liposomes, DEDPP-8/DOPE achieved the best transfection efficiency in four cell lines, and the transfection efficiency of DEDPP-8/DOPE in HeLa cell lines was 23.5-fold higher than that of Lipo2000, which is unusually high for small organic molecule-based nonviral vectors. Furthermore, excellent transfection efficiency of DEDPP-8/DOPE was obtained in the presence of serum, and the red fluorescence protein (RFP) gene was successfully transfected in zebrafish embryos. Both one- and two-photon fluorescence imaging clearly demonstrated the delivery process of plasmid DNA. This study demonstrated that gemini-type amphiphiles composed of a two-photon fluorophore core conjugated with triazole-[12]aneN3 via an ester bond afforded an unprecedentedly high transfection efficiency with excellent biocompatibility, which may provide new insights for the design and development of multifunctional nonviral gene vectors for imageable gene delivery.

5.
J Mater Chem B ; 8(17): 3869-3879, 2020 05 06.
Artigo em Inglês | MEDLINE | ID: mdl-32222754

RESUMO

With the aim to develop a novel multifunctional gene delivery system that may overcome the common barriers of gene transfection, near-infrared fluorescent triphenylamine-pyrazine was modified with a DNA condensing triazole-[12]aneN3 moiety through different length alkyl ester linkages to afford three new non-viral gene vectors, TDM-A/B/C. All compounds showed prominent solvatochromic fluorescence (Stokes shift of up to 383 nm) and two-photon absorption properties (σ2P to 101 GM), and exhibited strong aggregation-induced emission (AIE). Gel electrophoresis demonstrated that plasmid DNA was completely condensed at a concentration of 10 µM (TDM-A), 14 µM (TDM-B) and 16 µM (TDM-C), and released in esterase and acidic environment. SEM demonstrated that the three compounds were able to self-assemble and co-aggregate with DNA to form regular nanoparticles. Experiments demonstrated that TDM-A/B/C was able to integrate with DNA through electrostatic interactions and supramolecular stacking, and the short alkyl linkage favored the strong interaction with DNA. Among the three compounds, TDM-B showed the best luciferase and GFP transfection activities in the presence of DOPE, which were 156% and 310% higher than those of Lipo2000, respectively. The transfection process of DNA was clearly traced through one- and two-photon fluorescence microscopy imaging. Cellular uptake inhibition assay indicated that the DNA complex entered the cell mainly via clathrin-independent endocytosis. Furthermore, the in vivo transfection experiments of TDM-B/DOPE were successfully implemented in zebra fish embryos, and the GFP gene expression level was superior to that of Lipo2000 (200%). Finally, this study clearly unraveled that the length of the alkyl linkage affected the DNA condensation and transfection activity, which can serve as a base for the future rational design of non-viral gene vectors.

6.
ACS Appl Mater Interfaces ; 12(9): 10193-10201, 2020 Mar 04.
Artigo em Inglês | MEDLINE | ID: mdl-32045197

RESUMO

Tumor targeting provided more effective gene therapy. Bcl-2 is an oncogene, and Bcl-2 small interfering RNA (Bcl-2 siRNA) can inhibit its expression. Here, a fluorescent and gene-loading capacity vector DPL, derived from diketopyrrolopyrrole (DPP), was developed for Bcl-2 siRNA-targeted delivery and tumor imaging in vitro and in vivo. The vector DPL showed a significant emission enhancement after interacting with siRNA, which was used to track the gene transfer process. Compared to commercial transfection reagent Lipo 2000, DPL obviously downregulated the Bcl-2 protein expression and exhibited excellent antitumor efficacy with less Bcl-2 siRNA. Importantly, DPL can target tumors to transport Bcl-2 siRNA to tumor sites in vivo based on the enhanced permeability and retention (EPR) effect for effective in vivo tumor therapy. This work inspired us to design and synthesize a multifunctional gene vector for tumor targeting and gene therapy.

7.
ACS Appl Mater Interfaces ; 11(46): 42975-42987, 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31657894

RESUMO

Three nonviral gene vectors, TPA-BI-A/B/C, have been designed and synthesized by the combination of one or two hydrophilic [12]aneN3 moieties and two-photon fluorescent triphenylamine-benzylideneimidazolone (TPA-BI) units through different ester linkage. Spectroscopic characterization demonstrated that TPA-BI-A/B/C had strong aggregation-induced emissions (AIE), large Stokes shifts (230, 284, and 263 nm), and large two-photon absorption cross sections (δ2PA) (67, 592, and 80 GM). Gel electrophoresis indicated that the three compounds completely condensed DNA at 15 µM in the presence of DOPE, and showed the lipase- and pH-triggered reversible release of DNA and the fluorescent recognition of the different lengths of ssDNA and dsDNA. The optimal TPA-BI-C/DOPE-mediated luciferase and GFP activity was 146% and 290% higher than those of Lipo2000. The transfection process of DNA could be traced clearly through one- and two-photon fluorescence spectra, and displayed in a 3D-video. TPA-BI-C/DOPE successfully transfected the GFP gene into zebrafish, which was superior to Lipo2000 (192%). In conclusion, TPA-BI-C/DOPE is the first nonviral gene vector with the abilities of pH/lipase enzyme responsiveness, one/two-photon fluorescent tracking of intracellular delivery of DNA, and successful transfection in vivo and in vitro, even better than Lipo2000.


Assuntos
Corantes Fluorescentes , Técnicas de Transferência de Genes , Vetores Genéticos , Fosfatidiletanolaminas , Células A549 , Corantes Fluorescentes/química , Corantes Fluorescentes/farmacologia , Vetores Genéticos/química , Vetores Genéticos/genética , Vetores Genéticos/farmacologia , Humanos , Microscopia de Fluorescência por Excitação Multifotônica , Fosfatidiletanolaminas/sangue , Fosfatidiletanolaminas/farmacologia
8.
Ying Yong Sheng Tai Xue Bao ; 30(4): 1261-1268, 2019 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-30994287

RESUMO

To investigate the organic cultivation mode for high-yield and high-quality melon, we measured the growth of melon that grown in pots with different conditions of organic nutrient solution and irrigation. There were three irrigation frequencies of organic nutrient solution (8-time application, each time 750 mL per plant, F1; 12-time application, each time 500 mL per plant, F2; 16-time application, each time 375 mL per plant, F3) and two different irrigation amount per single plant (irrigating by 120% daily evapotranspiration (ET) before fruit enlargement, by 140%ET after fruit enlargement, W1; irrigating by 140%ET before fruit enlargement, by 160%ET after fruit enlargement, W2), following a randomized block trial design. The effects of those treatments on photosynthetic characteristics, yield, quality, fertilizer and water use efficiency of melon were investigated. The results showed that photosynthetic rate of melon leaves was significantly increased by more frequent and less amount of organic nutrient solution application. Fruit yield and water use efficiency were significantly improved under low irrigation condition. Fertilizer utilization was optimal under the treatments of high irrigation and medium organic nutrient solution frequency. Fruit quality was improved under less organic nutrient solution and relatively suitable water supply. A mathematical model of exponential function y=0.214e0.18x (R2=0.851) could be used to quantify the relationship between the vitamin C content of melon fruit and frequency of organic nutrient solution application. Considering the variables, such as yield, quality and water use efficiency, F3W1 treatment was recommended with the aim to improve fruit quality and optimal water use efficiency under promising yield, which could achieve high-yield and high-quality cultivation of organic melon in facilities.


Assuntos
Irrigação Agrícola/métodos , Cucurbitaceae/fisiologia , Fertilizantes , Nutrientes , Fotossíntese , Água
9.
Analyst ; 143(23): 5799-5804, 2018 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-30357116

RESUMO

A two-photon fluorescent probe, TP-2Bz, was designed and synthesized and it exhibited good targeting capabilities toward cell nuclei. In particular, TP-2Bz demonstrated a high selectivity to both G-quadruplex DNA and viscosity inside the nucleus with significant ratiometric enhancement in fluorescence, providing a specific, versatile imaging tool for analyzing the viscosity and G-quadruplex DNA in living cells.


Assuntos
Benzimidazóis/química , Núcleo Celular/química , DNA/análise , Corantes Fluorescentes/química , Quadruplex G , Benzimidazóis/efeitos da radiação , DNA/química , DNA/genética , Corantes Fluorescentes/efeitos da radiação , Células Hep G2 , Humanos , Imagem Óptica/métodos , Fótons , Viscosidade
10.
Int J Clin Exp Med ; 8(11): 21201-7, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26885055

RESUMO

OBJECTIVE: Growth differentiation factor-15 (GDF-15) has been identified as a strong biomarker of cardiovascular diseases; however, no evidence are available concerning the relationship of GDF-15 and atrial fibrosis in patients with atrial fibrillation (AF) and rheumatic heart disease (RHD). METHODS: Twenty patients with rheumatic heart disease were divided into two groups, 10 cases with AF and 10 cases with sinus rhythm (SR). Clinical data and blood samples were collected; left atrial appendage was taken by the surgeon in the process of valve replacement. Masson stained sections and mRNA levels of cardiac fibrosis biomarkers were used to determine the level of cardiac fibrosis, the expression level of GDF-15 was evaluated via immunohistochemistry, enzyme-linked immunosorbent assay (ELISA) and real-time polymerase chain reaction (PCR). RESULTS: Compared with SR group, more collagen deposited in the atrial tissue of AF group. The distribution of GDF-15 in the AF group was significantly higher than SR group (P<0.05). In addition, plasma GDF-15 level and mRNA level of GDF-15 in atrial tissue of AF showed the same trend as the result of immunohistochemistry. After linear correlation analysis, the expression level of GDF-15 was found to be positively related to the degree of cardiac fibrosis. CONCLUSION: GDF-15 might involve in the development and maintenance of atrial fibrosis in patients with atrial fibrillation and rheumatic heart disease, and GDF-15 could be used as a novel biomarker to evaluate myocardial fibrosis in the future.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA