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1.
Methods Mol Biol ; 2391: 171-184, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-34686985

RESUMO

Fusarium oxysporum is a cross-kingdom fungal pathogen that not only causes devastating plant vascular diseases but can also opportunistically infect humans. Here we describe two high-throughput screening assays, a resazurin cell viability assay and an optical density assay, to screen natural products from cultured plant cells with antifungal properties against a clinical isolate of F. oxysporum. After elicitation by applying methyl jasmonate or by co-culture with F. oxysporum, as an abiotic elicitor and a biotic elicitor, respectively, we identified three cell lines that produce materials that inhibit fungal growth. Our procedure validates the powerful potential of combining high-throughput methods for the discovery of novel anti-pathogenic leads.

2.
Food Chem ; 369: 130965, 2022 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-34492612

RESUMO

Panax notoginseng leaves (PNL) was considered as a promising functional food ingredient with abundant protopanaxdiol ginsenosides. In this study, the influence of different drying methods on chemical components in PNL was characterized by a newly developed heart-cutting 2D-LC-HRMS. Our data indicates that vigorous ginsenoside transformation occurs in PNL processed by sun-air drying and hot-air drying (HAD) at 50 °C, but not shade-air drying (SAD), HAD at 25 °C and steaming prior to drying (SD). Specifically, the main components of PNL, ginsenosides Rb3, Rc, Rb2, Rb1 and Rd, can be transformed into notoginsenosides Fd and Fe, ginsenoside Rd2, Gypenoside XVII and ginsenoside F2, respectively, by highly selective cleavage of ß-1,2-glucosidic linkage at the C-3 position. Only SD can inactivate the proteins that mediate this transformation. Different drying methods also greatly affect the quality of PNL products extracted by the conventional decoction method. These findings offer the scientific basis to design industrial drying methods for ensuring the quality of PNL.


Assuntos
Ginsenosídeos , Panax notoginseng , Panax , Cromatografia Líquida de Alta Pressão , Composição de Medicamentos , Ginsenosídeos/análise , Espectrometria de Massas , Folhas de Planta/química
3.
Cytokine ; 149: 155759, 2022 01.
Artigo em Inglês | MEDLINE | ID: mdl-34775109

RESUMO

BACKGROUND: Exploration of reliable biomarkers most likely to identify non-small cell lung cancer (NSCLC) patients at high risk for recurrence after surgery is needed. METHODS: Quantibody® Human Cytokine Antibody 6000 was used as screening tool to measure serum levels of 280 cytokines in ten healthy individuals and nine samples from three NSCLC patients before operation, after operation and postoperative recurrence. Selected cytokines were validated in two independent sets (89 patients before surgery, 69 patients after surgery and 40 patients with postoperative recurrence for each set) using ELISA method. The association of the selected cytokine with clinicopathologic features was also evaluated. RESULTS: Thirty-six cytokines were declined after surgery and again elevated when recurrence. We selected MIG to be further assessed in 2 validation sets, the mean value of serum MIG levels in 396 NSCLC patients was 253.42 ± 274.48 pg/mL and was significantly higher than the level in 60 healthy controls (47.65 ± 33.23 pg/mL, P < 0.0001). The serum MIG levels were 366.36 ± 324.04 pg/mL pre-operation, 134.04 ± 127.52 pg/mL post-operation and 208.05 ± 239.39 pg/mL in recurrence in NSCLC patients. The serum MIG levels were significant differences among NSCLC patients of pre-operation, post-operation and recurrence and controls (P < 0.0001). Moreover, Serum MIG levels were decreased markedly after operation and notably increased when disease relapsed (P < 0.0005). Serum MIG levels trend to be higher in patients with male gender, older age, smoking habit, poor tumor differentiation, and non-adenocarcinoma histology. CONCLUSIONS: These data indicated that MIG might be an indicator of postoperative recurrence and help to identify NSCLC patient who was easy to relapse after surgery.

4.
Food Chem ; 372: 131225, 2022 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-34614463

RESUMO

Water-in-oil (W/O) high internal phase emulsions (HIPEs) were prepared using polyglycerol polyricinoleate (PGPR) and polysaccharide blends consisting of konjac glucomannan (KGM) and octenyl succinic anhydride starch (OSA-starch). The formation, stability, and functionality of these emulsions were varied by adjusting the ratio of KGM and OSA-starch. Interfacial tension measurements indicated that the OSA-starch co-adsorbed to the water-oil interface with PGPR, which would have led to the formation of a polysaccharide-layer that helped prevent separation of the HIPEs. The centrifugal stability, rheological and microstructural results indicated that the W/O HIPEs exhibited well pH, ionic and thermal stability. The encapsulation efficiency, stability, and bioaccessibility of the EGCG in the W/O HIPEs were evaluated by using EGCG as a model hydrophilic nutraceutical. This study provides useful insights into the utilization of emulsion technology to reduce the fat content and improve the nutritional profile of foods with oily continuous phases, such as spreads.


Assuntos
Amido , Água , Emulsões , Interações Hidrofóbicas e Hidrofílicas , Reologia
5.
Cell Rep ; 37(5): 109912, 2021 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-34731622

RESUMO

Fetal growth restriction (FGR) increases the risk for impaired cognitive function later in life. However, the precise mechanisms remain elusive. Using dexamethasone-induced FGR and protein restriction-influenced FGR mouse models, we observe learning and memory deficits in adult FGR offspring. FGR induces decreased hippocampal neurogenesis from the early post-natal period to adulthood by reducing the proliferation of neural stem cells (NSCs). We further find a persistent decrease of Tet1 expression in hippocampal NSCs of FGR mice. Mechanistically, Tet1 downregulation results in hypermethylation of the Dll3 and Notch1 promoters and inhibition of Notch signaling, leading to reduced NSC proliferation. Overexpression of Tet1 activates Notch signaling, offsets the decline in neurogenesis, and enhances learning and memory abilities in FGR offspring. Our data indicate that a long-term decrease in Tet1/Notch signaling in hippocampal NSCs contributes to impaired neurogenesis following FGR and could serve as potential targets for the intervention of FGR-related cognitive disorders.

6.
Plant Dis ; 2021 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-34752129

RESUMO

Philodendron bipinnatifidum belongs to the Araceae family, as its graceful plant architecture and the function of purifying air and water, which has important ornamental value (Yu et al. 2019). P. bipinnatifidum is often cultivated in parks and along roadsides. In August 2019 and June 2020, leaf spot disease was observed on over 80% of P. bipinnatifidum plants in Qingxiushan Park (N22°47'23.35″, E108°23'4.26″), Nanning, Guangxi, China. The disease was also frequently observed on P. bipinnatifidum in some other places in Nanning. Symptoms began as small, round, brown spots. As the disease developed, the center of the lesions was sunken with a dark brown border (Fig. 1). Under severe conditions, some spots were joined into larger irregular spots, and even whole leaves died. For fungal isolation, small pieces (5 × 5 mm) were cut from the margin between lesion and healthy tissues of symptomatic leaves, disinfected with 75% ethanol for 10 s, 1% sodium hypochlorite solution for 1 min and washed by sterile water for three times. Over one hundred morphologically similar colonies with white mycelia and a dark green pigment were obtained after 5 days incubation on potato dextrose agar (PDA) at 25°C. Isolates GBZ6-1, GBZ9-1 and GBZ9-2 were selected for intensive study, and the mycelial growth rates of them averaged 14.5 mm/day, 14.6 mm/day and 13.2 mm/day, respectively. Isolates could produce orange conidia on PDA for 7 days. Conidia were elliptical, aseptate and colourless, with sizes of 15.4 ± 0.14 µm × 5.8 ± 0.1 µm, 15.6 ± 0.14 µm × 5.3 ± 0.1 µm and 15.0 ± 0.16 µm × 6.3 ± 0.1 µm for GBZ6-1, GBZ9-1 and GBZ9-2, respectively. Appressoria were mostly brown ovoid, conidial appressoria averaged 7.62-9.31 µm × 5.84-742 µm, and mycelial appressoria were 8.06-9.22 µm × 6.28-6.92 µm. Genomic DNA was extracted by the DNAsecure Plant Kit [Tiangen Biotech (Beijing) Co., Ltd] and the rDNA internal transcribed spacer region (ITS), actin (ACT), calmodulin (CAL), chitin synthase (CHS-1) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) genes (Weir et al. 2012) were sequenced. Phylogenetic analysis was performed using RAXML (Version 2.0) based on sequences of multiple loci (ITS, ACT, CAL, CHS-1, and GAPDH). The results showed that three isolates were identified as C. siamense (accessions MW116805-MW116816, for ACT, CAL, CHS-1 and GAPDH of GBZ6-1, GBZ9-1 and GBZ9-2; MW073391-MW073393 for ITS of GBZ6-1, GBZ9-1 and GBZ9-2). According to the cultural and conidial morphology, as well as phylogenetic analysis, these isolates were identified as Colletotrichum siamense. Pathogenicity tests were conducted on one-year-old plants. Young healthy leaves of three plants were artificially wounded by gently scratching with a sterile needle and 10 µl of conidial suspension (106 spores/ml) were applied to per wound site for each isolate. Wounded leaves were inoculated with 10 µl of water as controls. All plants were sprayed with water and covered with plastic bags to maintain high humidity. Symptomatic lesions were observed on the inoculated leaves after 15 days at 28 °C, whereas no symptoms were observed on the control leaves. To fulfill Koch's postulates, fungi were reisolated from symptomatic leaves and morphologically identical to the inoculated isolates (Xue et al. 2020). To our knowledge, this is the first report of leaf spot caused by C. siamense on P. bipinnatifidum worldwide. This research may accelerate the development of future epidemiological studies and management strategies for anthracnose caused by C. siamense on P. bipinnatifidum.

7.
Artigo em Inglês | MEDLINE | ID: mdl-34765004

RESUMO

Background: Acupoint sensitization is considered an important factor in the efficacy of acupoint therapy. This study aimed to evaluate the efficacy of acupressure in the prevention of stable angina pectoris using acupoints with different pressure-pain sensitivities. Methods: A total of 202 patients were enrolled and randomly assigned to a high-sensitivity group (HSG) (n = 109) in which patients received acupressure at the five acupoints with the highest sensitivity to pain and a low-sensitivity group (LSG) (n = 93) in which patients received acupressure at the five acupoints with the lowest sensitivity to pain. The duration of acupressure treatment was 4 weeks, and the patients were evaluated at baseline, week 4, and week 8. The primary outcome was a change in the frequency of angina attacks from baseline. The secondary outcomes included nitroglycerin consumption, the Canadian Cardiovascular Society classification, and the Seattle Angina Questionnaire score. Adverse events such as bleeding and subcutaneous haemorrhage were recorded in both groups. Results: The effect of acupressure compared with baseline on the prevention of angina pectoris in HSG was better than that in LSG at week 4 (incidence rate ratio (IRR): 0.691 and 95% confidence interval (CI): [0.569, 0.839]) and week 8 (IRR: 0.692 and 95% CI: [0.569, 0.839]). No significant difference between groups was found in the frequency of nitroglycerin consumption at week 4 (odds ratio (OR) = 0.863 and 95% CI: [0.147, 5.077]) or week 8 (OR = 1.426 and 95% CI: [0.211, 9.661]). Two themes in the questionnaire showed significantly different changes from baseline between the two groups. Scores on the angina frequency (AF) subscale had changed more from the baseline in the HSG at week 8 than in the LSG (mean difference (MD) = 3.807 and 95% CI: [0.673, 6.942]). Scores on the treatment satisfaction (TS) subscale had also changed more in the HSG than in the LSG at week 4 (MD = 3.651 and 95% CI: [0.327, 7.327]) and week 8 (MD = 4.220 and 95% CI: [0.347, 7.346]). One patient in the LSG reported bruising at the acupoint. No unexpected safety problems arose. Conclusions: This study showed that acupressure at acupoints with high sensitivity to pain may effectively reduce the frequency of stable angina pectoris episodes. This trial is registered with NCT03975140.

8.
Front Psychol ; 12: 701629, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34733199

RESUMO

Objective: To identify the prevalence of comorbid anxiety and depression (CAD) and analyze the relationship between CAD and sociodemographic and obstetric-related variables in pregnant and postpartum Chinese women during the COVID-19 pandemic. Methods: Participants were 2,237 pregnant and postpartum women (aged 19-47 years) who visited various medical institutions in China between February 28, 2020, and April 26, 2020. They were asked to complete an online survey assessing the anxiety and depression, obstetric characteristics, and sociodemographic variables. The women were grouped into the following categories in accordance with the Generalized Anxiety Disorder Scale-7 (GAD-7) and the Patient Health Questionnaire-9 (PHQ-9): (a) CAD, (b) "anxiety only," (c) "depression only," and (d) "no depression or anxiety." After estimating the prevalence of CAD, "anxiety only," and "depression only," we carried out chi-squared tests and multiple logistic regression analysis to examine the related factors between these groups of pregnant and postpartum Chinese women. Results: Comorbid anxiety and depression, "anxiety only," and "depression only," occurred in 6.3, 5.8, and 3.9% of participants, respectively. The prevalence rates of CAD during the first, second, and third trimesters of pregnancy and the postpartum period were found to be 7.4, 6.5, 5.7, and 8.2%, respectively. The factors that differed among the groups were age (p < 0.05), marital status (p < 0.001), level of education (p < 0.05), family support (p < 0.001), and total live births (p < 0.001). "Poor family support" (odds ratio (OR): 1.90; 95% confidence interval (CI): 1.30-2.78; p = 0.0009) and "no birth" (OR: 1.91; 95% CI: 1.32-2.75; p = 0.0006) remained significant factors for the CAD group, while "poor family support" (OR: 2.16; 95% CI: 1.34-3.47; p = 0.0015) remained a significant factor for the "depression only" group when their results were compared to those of the "no depression or anxiety" group in the multiple logistic regression analysis. Conclusion: Pregnant and postpartum Chinese women with poor family support and primipara are at high risk for CAD during the COVID-19 pandemic. These results support the need for targeted perinatal programs to address CAD in pregnant and postpartum women during the pandemic period.

9.
Mol Cell Endocrinol ; 541: 111507, 2021 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-34785282

RESUMO

Bisphenol A (BPA) is a common endocrine disruptor and a high-fat diet (HFD) also affects fertility. However, little is known about the long-term consequences of simultaneous exposure to BPA and a HFD on reproductive health. Herein, we assessed the effects of maternal exposure to BPA in combination with a HFD on reproductive function in subsequent generations of female mice and evaluated its effects on the hypothalamic-pituitary-gonadal axis. We found that the combination of maternal exposure to BPA and a HFD led to increased urine BPA levels, precocious puberty, altered estrous cyclicity, decreased follicle numbers, and altered hypothalamic Kiss1 methylation status in F1 and F2 mice. Therefore, we demonstrated that maternal exposure to BPA in combination with a HFD exerts a trans-generational effect on female reproduction.

10.
J Clin Pharm Ther ; 2021 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-34796971

RESUMO

WHAT IS KNOWN AND OBJECTIVE: An increasing number of targeted drugs have been used to treat advanced or metastatic gastric cancer (GC) and gastroesophageal junction cancer (GEJC). However, the optimal treatment efficacy of these drugs is still controversial. The aims of this study are to systematically summarize the efficacy and safety of current targeted drugs for advanced or metastatic GC and GEJC. METHODS: PubMed, EmBase, Cochrane Library, Web of Science and ClinicalTrials were searched for double-blind randomized controlled trials (RCTs) on GC and GEJC up to December 2019. Additionally, we updated the literature search from Jan, 1, 2020 to September 30, 2021. Narrative and quantitative analysis were performed to analyse the efficacy and safety. STATA 15.1 was used to identify publication bias, and the SUCRA (surface under the cumulative ranking) curve was conducted to rank the treatments for each outcome. RESULTS: A total of 27 RCTs with 9295 GC and GEJC patients treated by 19 drugs were included. SUCRA showed that regorafenib was the most likely to improve patients' progression-free survival (96.4%), followed by apatinib (90.7%), nivolumab (82.4%), everolimus (76.5%) and pertuzumab (68.5%). Meanwhile, apatinib (92.4%) was most likely to improve overall survival, followed by nivolumab (87.9%), regorafenib (72.5%), olaparib (67.7%) and lapatinib (63.2%). Additionally, neutropenia, diarrhoea and fatigue were the most common adverse events caused by these drugs, followed by pain, nausea, decreased appetite, anaemia and vomiting. WHAT IS NEW AND CONCLUSION: Regorafenib and nivolumab have higher efficacy and tolerability and are the most advantageous for advanced GC and GEJC. Moreover, apatinib has higher efficacy but lower tolerability. Everolimus and pertuzumab combined with chemotherapy have best secondary higher efficacy for progression-free survival and good tolerability. Lapatinib and olaparib combined with chemotherapy have moderate efficacy for overall survival and good tolerability.

11.
Food Chem ; 373(Pt B): 131435, 2021 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-34741971

RESUMO

Blue mussel (Mytilus edulis L.) is a popular, nutritional, and tasty mollusk. To better understand the composition of nutrients and improve further processing of the mussels, metabolomic approaches were used to analyze the free amino acids, 5'-nucleotides, and lipid compositions of different tissues. Our results showed that the viscera and gonad were rich in glutamine and glycine. Adenosine 5'-monophosphate, uridine 5'-monophosphate, guanosine 5'-monophosphate, and inosine 5'-monophosphate were abundant in the mantle, foot, and adductor muscle. Three main types of lipids, phospholipids (PLs), glycerides, and fatty acids (FAs), were semi-quantified. PLs were mainly distributed in the gonad of male mussels and viscera, gonad, and mantles of female mussels. FAs were relatively high in the viscera of males and in the gonad and viscera of females. The viscera of females were rich in phosphatidylcholine, such as 16:0/22:6 and 16:0/20:5. Triglycerides were the key lipids for distinguishing different tissues, especially 16:0/18:1/18:3 and 16:0/18:4/20:5.

12.
Phytother Res ; 2021 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-34847624

RESUMO

Alnustone, a diarylheptane compound, exhibits potent growth inhibition against hepatocellular carcinoma (HCC) BEL-7402 cells. However, the underlying mechanisms associated with its anticancer activity remain unknown. In the present study, we evaluated the anticancer effect of alnustone against several human cancers focused on HCC and the possible associated mechanisms. The results showed that alnustone significantly inhibited the growth of several cancer cells by CCK-8 assay. Alnustone markedly induced apoptosis and decreased mitochondrial membrane potential in BEL-7402 and HepG2 cells. Alnustone inhibited the expression of proteins related to apoptosis and PI3K/Akt/mTOR/p70S6K pathways and generated ROS production in BEL-7402 and HepG2 cells. Moreover, N-acetyl-L-cysteine (NAC, a ROS inhibitor) could significantly reverse the effects of alnustone on the growth inhibition of BEL-7402 and HepG2 cells and the expression of proteins related to apoptosis and PI3K/Akt/mTOR signaling pathway in HepG2 cells. Furthermore, alnustone significantly inhibited tumor growth of HepG2 xenografts, obviously induced apoptosis in the tumor tissues and improved the pathological condition of liver tissues of mice in vivo. The study provides evidence that alnustone is effective against HCC via ROS-mediated PI3K/Akt/mTOR/p70S6K pathway and the compound has the potential to be developed as a novel anticancer agent for the treatment of HCC clinically.

13.
Int J Clin Pract ; : e15007, 2021 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-34784092

RESUMO

OBJECTIVE: To apply the mini-clinical evaluation exercise (Mini-CEX) to orthopaedics and check its influence on clinical operational abilities and thinking abilities. METHODS: The original Mini-CEX was modified to fit orthopaedics, and another Mini-CEX was established to test interns' clinical operational abilities. A total of 39 interns had to complete two types of Mini-CEX twice, once at the beginning and once at the end of the internship. Clinical supervisors collected all the scores and analysed the differences in the average scores between the first and second assessments. The interns were divided into Qualified teacher group and Excellent teacher group according to their Mini-CEX scores. RESULTS: The results of the Mini-CEX examination of the two groups were compared. Researchers found a significant difference between the two assessments on seven domains (all P < 0.05). The scores at the end were higher than those at the beginning, which indicated that the interns' clinical thinking and operational abilities had improved. The average scores of the interns in the Excellent teacher group were significantly higher than those of interns in the Qualified teacher group. CONCLUSIONS: The modified Mini-CEX is suitable for orthopaedic education and could help cultivate interns' clinical thinking ability.

14.
J Med Virol ; 2021 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-34825712

RESUMO

OBJECTIVES: This study compared the willingness to receive human papillomavirus (HPV) vaccination and the influencing factors between male and female university students who had never been vaccinated against HPV in China. METHODS: University students were recruited from seven universities in China. A self-administered questionnaire was used to collect information about demographic characteristics, willingness to HPV vaccination, and possible influencing factors including demographics, knowledge, and attitude toward HPV vaccination. The Chi-square test was used to test the difference between males and females' knowledge and attitudes to HPV vaccination. Multivariate logistic regression was employed to detect the factors associated with willingness to HPV vaccination. RESULTS: A total of 7335 university students participated in the survey (3570 males and 3765 females). Over 70% of the participants had previously received sex education and knowledge. The average age of sexual debut was 17.5 years old. More male students reported acceptance of premarital sex than female students did (χ2 =708.458, p<0.001). Female students had better knowledge and attitudes to HPV in general, while male students perceived a higher chance of being infected by HPV compared with female students (χ2 =6.047, p=0.014). The level of knowledge (Male: AOR=1.935, 95%CI:1.516-2.470; Female: AOR=1.227, 95%CI:1.055-1.428) and receiving sex education (Male: AOR=1.414, 95%CI:1.109-1.804; Female: AOR=1.289, 95%CI:1.064-1.562) were indicators of the HPV vaccination willingness for both genders. For male students, those who had ever inoculated optional self-paid vaccines were more likely to receive the HPV vaccine than those who hadn't (AOR=1.567, 95%CI: 1.242-1.977). Female students were more willing to be vaccinated against HPV, if they had higher living expenses (AOR=1.395 and 3.717, 95%CI: 1.071-1.426 and 1.776-7.752), relatives or friends had certain cancer (AOR=1.290, 95%CI: 1.095-1.518), ever had sexual experiences (AOR=2.628, 95%CI: 1.788-3.863), and had ever consulted on HPV vaccination issues (AOR=1.612, 95%CI: 1.367-1.901). CONCLUSIONS: In China, more active education should be provided to improve university students' knowledge and attitudes on HPV and HPV vaccination. Including HPV vaccine uptake for both males and females at recommended ages in National Immunization Program would be the most cost-effective way to prevent HPV infection. This article is protected by copyright. All rights reserved.

15.
Mediterr J Hematol Infect Dis ; 13(1): e2021060, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34804434

RESUMO

Objective: This study intends to investigate the prognostic risk factors of bloodstream infection in Beijing. Methods: This study is a clinical retrospective study. Four hundred forty-six patients with community-onset bloodstream infections (COBSI), admitted to the emergency department and inpatient department of Beijing Jishuitan Hospital from January 1, 2015, to December 31, 2019, were selected as the main research objects. According to whether the patient survives for 100 days or not, 363 cases were in the survival group, and 83 cases were in the death group. By analyzing the clinical data of the two groups of patients, the epidemiology, clinical characteristics, bacterial resistance, and risk factors affecting the prognosis of the patients were analyzed. Results: A total of 446 pathogenic bacteria were isolated in this study, including 324 Gram-negative (G-) bacteria (72.6%), 121 Gram-positive (G+) bacteria (27.1%). The results of the study showed that there were significant differences in MDR, initial antibiotic use, solid tumor, CKD, septic shock, acute liver injury, AKI, central venous catheter, urinary catheter, blood replacement therapy, invasive operation, and use of three or more antibiotics between the two groups (p<0.05). The multiple logistic regression analysis showed that solid tumors (OR=3.339, 95% CI: (1.441, 7.734), p=0.005), combined septic shock (OR=20.729, 95% CI: (10.235, 41.982), p<0.001), indwelling catheters (OR=3.556, 95% CI: (1.538, 8.222), p=0.003) and continuous venovenous hemofiltration (CVVH, OR=19.548, 95% CI: (8.724, 35.641), p=0.003) are independent risk factors affecting the prognosis of COBSI patients. Appropriate initial antibiotic therapy is a protective factor affecting the prognosis of COBSI patients. Conclusion: Solid tumors, combined septic shock, indwelling catheters, CVVH are independent risk factors affecting the prognosis of COBSI patients.

16.
Artigo em Inglês | MEDLINE | ID: mdl-34802765

RESUMO

Colorimetric biosensors, based on enzyme-like nanomaterials, have come into the spotlight in virtue of their visual detection. Herein, a daisy-like zeolitic imidazolate framework-67/reduced grapheme oxide (ZIF-67/rGO) nanozyme with unique 3D hierarchical structures has been designed to realize visual detection of hydrogen peroxide (H2O2) that is recognized as a strong oxidizing agent or reactive oxygen species associated with oxidative stress in biological systems. The daisy-like ZIF-67/rGO is prepared by a facile one-step liquid-phase method conducted under room temperature. The successful introduction of rGO endows the daisy-like ZIF-67/rGO nanozyme with abundant porous structure, high specific surface area, and good charge transfer capability, which significantly accelerates the adsorbability and recognition towards the substrates and the oxidation rate of TMB-H2O2 reaction, and thus improving the nanozyme activity observably. It is conductive to nanozyme-modulated H2O2 determination. The established colorimetric biosensor platform based on ZIF-67/rGO nanozyme exhibits remarkable sensitivity and high specificity for the application in visual detection of H2O2. The detection limit of ZIF-67/rGO-based biosensor platform is as low as 3.81 µM, which is nearly 8 times lower than that of ZIF-67-based biosensor platform. Moreover, its potential applicability as an ideal platform for colorimetric biosensors is demonstrated by testing the concentration of H2O2 in milk samples, which sheds light on the promising application of the proposed biosensing system in point-of-care detection.

17.
J Org Chem ; 2021 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-34807586

RESUMO

The development of a novel environmental benign and sustainable synthetic method for highly efficient construction and direct C-H functionalization of N-heterocycles remains a pivotal central research topic for organic and medicinal chemistry. Herein, a novel visible-light-enabled biomimetic aza-6π electrocyclization for efficient assembly of diverse pyridines and further tandem Minisci-type reaction were developed. A broad spectrum of polysubstituted picolinaldehydes were readily constructed with high efficacy and good functional group tolerance under metal- and oxidant-free conditions.

18.
Ann Vasc Surg ; 2021 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-34808262

RESUMO

OBJECTIVE: Currently, no standard treatment has been established for the total lesion in patient with Marfan Syndrome (MFS). This case report aims to present a total aortic and branches repair with hybrid therapy in a young patient with MFS. METHODS: Clinical data including imaging manifestation, surgical document, and follow-up results were retrospectively collected and presented. RESULTS: A young patient with MFS underwent multi-stage endovascular aortic management and open surgical repair. On-the-table fenestration technique was applied to reconstruct the branches of the abdominal aorta. A bypass from superior mesenteric artery to celiac trunk was performed. A Bentall operation was conducted to repair his ascending aorta and aortic valves. Finally, in situ fenestration technique was adopted to recanalize the branches of aortic arch. The 18-month follow-up computed tomography angiography demonstrated patency of all the aorta branches. CONCLUSION: It may be feasible to perform the total aortic and branches repair with hybrid therapy in patients with MFS.

19.
J Adv Nurs ; 2021 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-34812513

RESUMO

AIM: The present study aims to explore the research hot spots, development trends and knowledge structure of dignity in the nursing field. DESIGN: Quantitative and co-word biclustering analysis were used. METHODS: Articles on dignity care published from 01 Jan 2011 to 31 Dec 2020, were retrieved from PubMed. The extracted Medical Subject Headings (MeSH) terms were quantitatively analysed using Bibliographic Item Co-occurrence Matrix Builder software. To determine the hot spots, a biclustering analysis was completed using gCluto1.0 software. A strategic diagram and a social network analysis (SNA) were used to reveal trends in the theme and knowledge structure. RESULTS: In the parameters of the retrieval strategy, a total of 1977 papers were included in the present study. Amongst all the extracted MeSH terms, 27 high-frequency MeSH terms were identified, and the hot spots were grouped into five categories. These were namely dignity in: (1) dementia care, (2) palliative care, (3) older people care, (4) healthcare and (5) clinical nursing. In the strategic diagram, the study of dignity in clinical nursing was active and should become an emerging field of research in the near future. CONCLUSIONS: Based on the co-word biclustering of dignity care over the past 10 years, five hot spots were identified, and it was predicted that research on dignity in clinical nursing would be the main trend in future studies. Amongst the five themes it was interesting to note that dignity in dementia and palliative care are core priorities to which scholars should pay more attention. IMPACT: In recent years, dignity-conserving care has been highly valued, however, there are few relevant bibliometric articles that can be referenced on this topic. The present study was considered to offer novel insights into research on dignity in nursing and could be a reliable reference point for researchers when launching new projects.

20.
Curr Alzheimer Res ; 2021 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-34819006

RESUMO

BACKGROUND: As the most basic material, synthetic human Amyloid-ß (1-42) (Aß42) pep- tides from different manufacturers have been widely used. Their aggregation ability is vital to the reliability, repeatability and comparability of studies on Aß42 physiology and pathology. However, it has not been evaluated and compared. OBJECTIVES: To analyze the consistency of the aggregation ability of 5 commercially available Aß42 peptides. METHODS: 5 Aß42 peptides represented as A, B, C, D and E were pretreated by HFIP. The pretreated Aß42 peptides were dissolved in Thioflavin T (ThT) solution, and their aggregation kinetics was monitored for 30 h with the aggregation kinetics test. Meanwhile, the pretreated peptides were ag- gregated in phosphate buffered saline. After aggregated for 12 h, they were detected by methods of ThT fluorescence, far-UV circular dichroism (CD), SDS-PAGE, western blot, and transmission electron microscopy (TEM), respectively. After aggregation for 8 h and 12 h, their cytotoxicity to SH-SY5Y cells was further evaluated using Cell Counting Kit-8. RESULTS: For aggregation kinetics, peptides A, C and E remained low level curves, while peptides B and D presented typical sigmoidal kinetics curves. In CD measurement, the aggregates of pep- tides B and D showed relatively high negative CD peaks with the height of -8.09 mdeg and -14.37 mdeg, while the height of peptides A, C and E was -1.04, -3.55, and -3.88. In ThT assay, relative fluorescence intensity of the aggregates of peptides B and D were 7.79 and 8.82, higher than 1.19, 1.71, and 2.70 of peptide A, C and E, respectively. In SDS-PAGE, all aggregates contained monomers and eleven polymers. Moreover, peptide B-E presented a trapezoidal distribution from dimers to trimers, and peptide A aggregated to dimers. By western blot, the bands of monomers re- mained in all aggregates. Furthermore, peptides B and D aggregated to dimers and trimers, pep- tides A and C only aggregated to dimers, and peptide E showed a strong band of trimers. By TEM, protofibrils were observed only in peptide B, while substantial spherical aggregates were formed in other peptides. Additionally, peptides B, D and E exhibited higher cytotoxicity after being aggregat- ed for 8 h, whereas peptides A, B and D presented relatively high cytotoxicity after 12-hour aggre- gation. CONCLUSION: Commercially available Aß42 peptides showed obvious differences in aggregation abil- ity, which should arouse enough attention in the field of basic study related to Aß42. The aggrega- tion ability evaluation with the various assay methods has some discrepancies, and it is highly ur- gent to establish a reasonable and uniform measurement strategy.

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