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1.
Am J Otolaryngol ; : 102450, 2020 Feb 27.
Artigo em Inglês | MEDLINE | ID: mdl-32183991

RESUMO

OBJECTIVE: Nasal septal deviation (NSD) has a significant impact on patients' quality of life; however, there have been limited studies examining the psychological status of NSD patients. In this study, symptoms of depression and anxiety were investigated between NSD patients and controls using a self-report questionnaire. METHODS: A case-control study design was used to evaluate the psychological burden of NSD in patients who visited the general hospital. The control group comprised of ENT outpatients without a history of chronic nasal disease. The Zung Self-rating Anxiety/Depression Scale (SDS/SAS) was used to evaluate the prevalence and severity of anxiety and depression between the NSD and control group. RESULTS: Seventy-six patients with NSD and 79 control patients were enrolled in the study. We found that depression and anxiety, as well as the co-morbidity of depression with anxiety, were more common in the NSD group in comparison to the control (39.5% vs 22.8%, p = 0.025; 38.2% vs 15.2%, p = 0.001; and 27.6% vs 11.4%, p = 0.011, respectively). The average SDS and SAS score was higher in NSD patients compared to controls (SDS: 49.7 ± 13.1 vs 45.2 ± 10.4, p = 0.019 and SAS: 48.1 ± 11.6 vs 41.3 ± 9.3, p < 0.001, respectively), and NSD patients were found to have more severe levels of anxiety and depression. CONCLUSION: Depression and anxiety are more common and severe in patients with NSD. Therefore, psychological distress should be taken into consideration during the diagnostic and therapeutic process for patients with NSD.

2.
Analyst ; 2020 Mar 03.
Artigo em Inglês | MEDLINE | ID: mdl-32124870

RESUMO

A methodology is presented for the determination of four pyrethroid (PYR) preservatives in wooden food contact materials (FCMs) using direct analysis in real time (DART) coupled with quadrupole-Orbitrap high-resolution mass spectrometry (Q-Orbitrap HRMS). The sampling mode and critical parameters of the DART-Q-Orbitrap HRMS protocol were systematically investigated. Good linearity was achieved for the four analytes with correlation coefficients all greater than 0.99. The limits of detection (LODs) and limits of quantitation (LOQs) of the method were in the range of 0.04-0.20 mg kg-1 and 0.10-0.50 mg kg-1, respectively. The mean recoveries ranged from 72.1% to 82.7% with relative standard deviations (RSDs) from 5.2% to 11.8% at three spiked levels. The developed method was proved to be suitable for rapid screening of PYRs in complex wooden FCM samples to ensure product safety and consumer health.

3.
BMC Pregnancy Childbirth ; 20(1): 71, 2020 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-32013903

RESUMO

BACKGROUND: Salpingectomy-associated uterine rupture during intrauterine pregnancy is rare in the clinic. We report a case of pregnancy with bilateral rupture of the uterine horns after bilateral salpingectomy. CASE PRESENTATION: A 30-year-old woman of Han ethnicity presented with right epigastric pain at 28 weeks and 6 days of gestation. Examination by colour Doppler ultrasound showed the following: "Twin live births with normal foetal umbilical artery blood flow indexes and a 183 mm × 112 mm anechoic zone in the right front of the uterus". Initially, we made an incorrect judgement wherein we considered the amniotic sac that was protruding into the abdominal cavity to be an adnexal cyst. Fortunately, the diagnosis of uterine rupture was confirmed before the protruded amniotic sac broke. The mother did not bleed much, and the twin foetuses survived in our case. CONCLUSION: A previous history of salpingectomy via laparoscopy could be a risk factor for uterine rupture in pregnant women. Attention should be paid to rare complications of pregnancy. To avoid adverse events, we should pay special attention to women with a history of laparoscopic salpingectomy who complain about abdominal discomfort and offer them a relevant ultrasound examination.

4.
Physiology (Bethesda) ; 35(2): 86-95, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-32024428

RESUMO

In cancer cells, glucose is primarily metabolized to pyruvate and then to lactate in the cytosol. By allowing the conversion of pyruvate to acetyl-CoA in mitochondria, melatonin reprograms glucose metabolism in cancer cells to a normal cell phenotype. Acetyl-CoA in the mitochondria also serves as a necessary co-factor for the rate-limiting enzyme in melatonin synthesis, thus ensuring melatonin production in mitochondria of normal cells.

5.
Eur Radiol ; 2020 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-32078014

RESUMO

OBJECTIVE: The current study aimed to evaluate the clinical practice for hemodynamic tissue signature (HTS) method in IDH genotype prediction in three groups derived from high-grade gliomas. METHODS: Preoperative MRI examinations of 44 patients with known grade and IDH genotype were assigned into three study groups: glioblastoma multiforme, grade III, and high-grade gliomas. Perfusion parameters were analyzed and were used to automatically draw the four reproducible habitats (high-angiogenic enhancing tumor habitats, low-angiogenic enhancing tumor habitats, infiltrated peripheral edema habitats, vasogenic peripheral edema habitats) related to vascular heterogeneity. These four habitats were then compared between inter-patient with IDH mutation and their wild-type counterparts at these three groups, respectively. The discriminating potential for HTS in assessing IDH mutation status prediction was assessed by ROC curves. RESULTS: Compared with IDH wild type, IDH mutation had significantly decreased relative cerebral blood volume (rCBV) at the high-angiogenic enhancing tumor habitats and low-angiogenic enhancing tumor habitats. ROC analysis revealed that the rCBVs in habitats had great ability to discriminate IDH mutation from their wild type in all groups. In addition, the Kaplan-Meier survival analysis yielded significant differences for the survival times observed from the populations dichotomized by low (< 4.31) and high (> 4.31) rCBV in the low-angiogenic enhancing tumor habitat. CONCLUSIONS: The HTS method has been proven to have high prediction capabilities for IDH mutation status in high-grade glioma patients, providing a set of quantifiable habitats associated with tumor vascular heterogeneity. KEY POINTS: • The HTS method has a high accuracy for molecular stratification prediction for all subsets of HGG. • The HTS method can give IDH mutation-related hemodynamic information of tumor-infiltrated and vasogenic edema. • IDH-relevant rCBV difference in habitats will be a great prognosis factor in HGG.

6.
FEBS Open Bio ; 2020 Feb 06.
Artigo em Inglês | MEDLINE | ID: mdl-32027103

RESUMO

Nearly half of human cancers harbor p53 mutations, and mutant p53 (mutp53) promotes carcinogenesis, metastasis, tumor recurrence and chemoresistance. mutp53 is observed in 30% of breast carcinomas, including triple-negative breast cancer (TNBC), and thus mutp53 is a promising target for treatment of TNBC. In this study, we investigated the effect of a phosphatidylinositide 3 kinase/mammalian target of rapamycin dual inhibitor, NVP-BEZ235 (BEZ235), on two TNBC cell lines with mutp53: MDA-MB-231 and MDA-MB-468. Cell growth, migration and colony-formation abilities were detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide, scratch assay, transwell and soft agar assay, revealing that BEZ235 can inhibit the growth, migration and colony-formation abilities of TNBC cells. In addition, BEZ235 caused degradation of mutp53 in these cells. We investigated the underlying mechanism by inhibiting proteasome function using MG132 and inhibiting autophagy using 3-methyladenine and shRNAs. We observed that BEZ235 may induce autophagy through repression of the Akt/mammalian target of rapamycin signaling pathway. The observed interplay between mutp53 and autophagy in TNBC cells was examined further by knockdown of ATG5 and ATG7, revealing that degradation of mutp53 induced by BEZ235 may be independent of the ubiquitin-proteasome pathway and autophagy mediated by ATG5 and ATG7. Moreover, we found evidence of positive feedback between mutp53 and autophagy in TNBC cells. In conclusion, BEZ235 may exert antitumor effects against TNBC cells by targeting mutp53, and this may have implications for the development of future therapies.

7.
J Fluoresc ; 2020 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-32020436

RESUMO

Excessive secretion of PTH leads to disturbance of calcium and phosphorus metabolism in the body, which promotes bone, kidney, digestive system and nervous system diseases. Due to the short half-life of PTH, it becomes a difficult issue for PTH detection in the clinical diagnosis field. We explored a competitive immunofluorescent sensing mode based on FRET of two-color CdTe QDs for ratiometric PTH 1-84 antigen detection. The FRET effect and ratiometric fluorescence between the two-color CdTe QDs motivated accurate quantification of PTH 1-84 antigen concentration from 0.01 ng mL-1 to 0.08 ng mL-1 with a limit of detection of 3 pg mL-1. More importantly, under UV irradiation, samples with different concentrations of PTH 1-84 antigen achieved fluorescence visualization, which provides huge possibility for the practical application of PTH 1-84 antigen point-of-care detection.

8.
Anal Bioanal Chem ; 412(8): 1893-1899, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32016568

RESUMO

Superoxide dismutase (SOD), also known as liver protein, is a substance widely distributed in various biological cells. It has the function of catalyzing the disproportionation reaction of superoxide free radicals. SOD can form an antioxidant chain together with peroxidase, catalase, and other substances in the body of organisms, and thus, is one of the indispensable important substances in the body of organisms. In this work, we provided a simple and fast visual electrochemiluminescence (ECL) sensor for SOD detection. CuInZnS quantum dots (QDs) worked as the ECL luminophore with hydrogen peroxide as co-reactant. In the sensing process, SOD and CuInZnS QDs on a glassy carbon electrode (GCE) competed with each other for hydrogen peroxide to produce superoxide during electrochemical luminescence, thus quenching the ECL signal of CuInZnS QDs. The proposed sensor can quantify SOD with a limit of detection (LOD) of 0.03 µg/mL. In addition, the change in the CuInZnS QDs ECL signal was easily observed with a smartphone camera. The results indicated that this sensor could effectively work in the detection of SOD in human blood. Graphical abstract.

9.
Int J Biol Sci ; 16(5): 739-751, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32071545

RESUMO

The highly conserved protease TASP1 not only takes part in critical site-specific proteolysis, but also plays an important role in numerous liquid and solid malignancies. However, the TASP1 expression and its biological regulation function in malignant gallbladder carcinoma (GBC) remain fully unknown. Here we observed that TASP1 levels were substantially overexpressed in GBC samples compared with non-tumor tissues. High TASP1 level was closely associated with T stage and metastasis, and was also correlated with poor prognosis in GBC patients. The depletion of TASP1 inhibited GBC cell proliferation and metastasis in vitro and in vivo. Furthermore, we first revealed that FAM49B had biological function and was positively regulated by TASP1 activating PI3K/AKT signaling pathway in GBC. At the same time, FAM49B also promoted GBC cell proliferation and migration. Inhibition of PI3K/AKT with LY294002 or FAM49B expression abrogated Myc-TASP1/Lv-shTASP1-induced GBC cell proliferation and motility. In conclusion, these findings demonstrate that TASP1 is critical for GBC progression via TASP1-PI3K/AKT-FAM49B axis and it may be a novel prognostic factor. The therapeutic targeting TASP1 may be a potential treatment approach for GBC patients.

10.
Rapid Commun Mass Spectrom ; : e8731, 2020 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-31960493

RESUMO

RATIONALE: Linear ion traps with simplified structure have been widely used in miniaturized mass spectrometers. However, linear ion traps usually have low ion detection efficiency when used in a miniaturized mass spectrometer, in which only one ion detector can be installed. To solve this problem, an asymmetric radio frequency voltage was applied to introduce the asymmetric electric fields in the trapping volume of the linear ion traps, which would lead to unidirectional ion ejection. METHODS: An asymmetric RF voltage was applied on both half round-rod electrode and triangular electrode linear ion traps, and their performances including unidirectional ion ejection efficiency and mass resolution were evaluated by computer simulation. The relationship between asymmetric RF voltage difference δ and internal electric field distribution was investigated, and the impact of δ and resonance excitation signal frequency on the unidirectional ion ejection efficiency and mass resolution was also explored. RESULTS: A unidirectional ion ejection efficiency of around 83% and a mass resolution of over 2700 were achieved with δ=7% in both half round-rod electrode and triangular electrode linear ion traps. CONCLUSIONS: By applying an asymmetric RF voltage, the ion detection efficiency could be significantly improved without any change of the structures of existing linear ion traps. This method provided a simple and general solution for improving the ion detection efficiency and sensitivity of miniaturized linear ion trap mass spectrometers.

11.
Cell Mol Life Sci ; 2020 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-31970423

RESUMO

Melatonin has the ability to intervene in the initiation, progression and metastasis of some experimental cancers. A large variety of potential mechanisms have been advanced to describe the metabolic and molecular events associated with melatonin's interactions with cancer cells. There is one metabolic perturbation that is common to a large number of solid tumors and accounts for the ability of cancer cells to actively proliferate, avoid apoptosis, and readily metastasize, i.e., they use cytosolic aerobic glycolysis (the Warburg effect) to rapidly generate the necessary ATP required for the high metabolic demands of the cancer cells. There are several drugs, referred to as glycolytic agents, that cause cancer cells to abandon aerobic glycolysis and shift to the more conventional mitochondrial oxidative phosphorylation for ATP synthesis as in normal cells. In doing so, glycolytic agents also inhibit cancer growth. Herein, we hypothesize that melatonin also functions as an inhibitor of cytosolic glycolysis in cancer cells using mechanisms, i.e., downregulation of the enzyme (pyruvate dehydrogenase kinase) that interferes with the conversion of pyruvate to acetyl CoA in the mitochondria, as do other glycolytic drugs. In doing so, melatonin halts the proliferative activity of cancer cells, reduces their metastatic potential and causes them to more readily undergo apoptosis. This hypothesis is discussed in relation to the previously published reports. Whereas melatonin is synthesized in the mitochondria of normal cells, we hypothesize that this synthetic capability is not present in cancer cell mitochondria because of the depressed acetyl CoA; acetyl CoA is necessary for the rate limiting enzyme in melatonin synthesis, arylalkylamine-N-acetyltransferase. Finally, the ability of melatonin to switch glucose oxidation from the cytosol to the mitochondria also explains how tumors that become resistant to conventional chemotherapies are re-sensitized to the same treatment when melatonin is applied.

12.
Biophys Chem ; 258: 106317, 2020 03.
Artigo em Inglês | MEDLINE | ID: mdl-31918025

RESUMO

The antimicrobial activity of amphotericin B (AmB) depends on its interaction with ergosterol-containing cell membranes of fungus. Cholesterol is a sterol in mammalian cell membrane, and its structure is very similar to ergosterol, which caused to the toxic of amphotericin B to mammalian or human cell membranes. Even so, it is still the gold standard for the treatment of fungal infections. The mechanism of its toxicity to mammalian cell membrane has become a hot topic. The toxicity mechanism of amphotericin B on the cell membrane is also related to the phospholipids on the membrane. The effects of saturated and unsaturated fat chains on the interaction of amphotericin B with phospholipid monolayers containing cholesterol or ergosterol were studied at the molecular level using an air-water interface monolayer model. Both atomic force microscope and Brewster angle microscope were used to observe the surface morphology of the monolayer. The analysis of limiting molecular area suggested that the interaction between AmB and the two kinds of sterol is significantly different on the unsaturated lipid monolayer. According to the elastic modulus, the AmB molecules can increase the compressibility or viscoelasticity of the phospholipid/sterol monolayer. However, this impact of AmB on the DOPC/sterol monolayer containing ergosterol was stronger than that containing cholesterol at 25 ~ 50 mN/m. While this impact of AmB on the DPPC/sterol monolayer containing cholesterol was stronger than that containing ergosterol at 32 ~ 56 mN/m. The excess Gibbs free energy of the monolayer showed that, in the presence of saturated fat chain, amphotericin B could make the molecules of the DPPC/cholesterol monolayer and the DPPC/ergosterol monolayer arrange more closely and make intermolecular interaction stronger. There was no significant difference between DPPC/cholesterol monolayer and DPPC/ergosterol monolayer. However, in the presence of unsaturated chain, the effects of amphotericin B on the DOPC/cholesterol monolayer and the DOPC/ergosterol monolayer were significantly different. Amphotericin B made the molecular arrangement of DOPC/ergosterol monolayer more loosed, and the intermolecular force weakened at 5-35 mN/m. AFM images reflect that AmB can perforate the phospholipid-ergosterol monolayer, which was no significant correlation with saturation of the lipid monolayer. But the areas of dark areas shaped holes on the DPPC/ergosterol monolayer were larger than that on the DOPC/ergosterol monolayer. The adsorption of amphotericin B on lipid/sterol monolayer suggests that the orientation of amphotericin B may be different when it is inserted into the monolayer of phospholipid-sterol in the presence of saturated or unsaturated chains. The results are helpful to understand the complex mechanism of toxicity of amphotericin B to cell membrane.


Assuntos
Anfotericina B/química , Antibacterianos/química , Colesterol/química , Ergosterol/química , Fosfolipídeos/química , Ar , Humanos , Tamanho da Partícula , Pressão , Propriedades de Superfície , Água/química
13.
Anal Chim Acta ; 1098: 66-74, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31948588

RESUMO

A streamlined analytical workflow was developed for the analysis of infant drink samples using a miniature mass spectrometry system preceded by solid-phase microextraction (SPME) and extraction nano-electrospray ionization. Potential chemical contaminants in infant drinks (milk, lactic acid bacteria beverage, and fruit juice) were extracted and enriched using a custom-made stainless-steel SPME probe, which was coated with a thin layer of polyaniline and multi-walled carbon nanotubes nanocomposites (PANI/MWCNTs) by electrochemical deposition. The resulting porous microstructure has a larger surface area and enhanced microextraction efficiency, with enrichment factors ranging from 3055 to 8695 for exemplary analytes of antibiotics, bisphenol A, and perfluorinated compounds. The enriched analytes on the electrochemically fabricated SPME probe were simultaneously desorbed and ionized within a pulled glass capillary by extraction nano-electrospray ionization. The ionized species were subjected to instrumental analysis on a miniature ion trap mass spectrometer with adequate tandem mass spectrometry capability. The developed method was optimized and validated in terms of sensitivity, linearity, repeatability, and recovery. The integrated experimental protocol combining SPME, ambient ionization, and miniature mass spectrometry is promising for rapid, on-site screening of hazardous substances in food to ensure consumer health.

14.
Artigo em Inglês | MEDLINE | ID: mdl-31893511

RESUMO

Metal-organic framework (MOF) glasses are promising candidates for membrane fabrication due to their significant porosity, the ease of processing, and most notably, the potential to eliminate the grain boundary that is unavoidable for polycrystalline MOF membranes. Herein, we developed a ZIF-62 MOF glass membrane and exploited its intrinsic gas-separation properties. The MOF glass membrane was fabricated by melt-quenching treatment of an in situ solvothermally synthesized polycrystalline ZIF-62 MOF membrane on a porous ceramic alumina support. The molten ZIF-62 phase penetrated into the nanopores of the support and eliminated the formation of intercrystalline defects in the resultant glass membrane. The molecular sieving ability of the MOF membrane is remarkably enhanced via vitrification. The separation factors of the MOF glass membrane for H2 /CH4 , CO2 /N2 and CO2 /CH4 mixtures are 50.7, 34.5, and 36.6, respectively, far exceeding the Robeson upper bounds.

15.
Nature ; 577(7791): 531-536, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31942070

RESUMO

The hippocampus is an important part of the limbic system in the human brain that has essential roles in spatial navigation and the consolidation of information from short-term memory to long-term memory1,2. Here we use single-cell RNA sequencing and assay for transposase-accessible chromatin using sequencing (ATAC-seq) analysis to illustrate the cell types, cell linage, molecular features and transcriptional regulation of the developing human hippocampus. Using the transcriptomes of 30,416 cells from the human hippocampus at gestational weeks 16-27, we identify 47 cell subtypes and their developmental trajectories. We also identify the migrating paths and cell lineages of PAX6+ and HOPX+ hippocampal progenitors, and regional markers of CA1, CA3 and dentate gyrus neurons. Multiomic data have uncovered transcriptional regulatory networks of the dentate gyrus marker PROX1. We also illustrate spatially specific gene expression in the developing human prefrontal cortex and hippocampus. The molecular features of the human hippocampus at gestational weeks 16-20 are similar to those of the mouse at postnatal days 0-5 and reveal gene expression differences between the two species. Transient expression of the primate-specific gene NBPF1 leads to a marked increase in PROX1+ cells in the mouse hippocampus. These data provides a blueprint for understanding human hippocampal development and a tool for investigating related diseases.

16.
Environ Pollut ; 260: 114042, 2020 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-32000029

RESUMO

Urbanization and industrialization have elevated metal concentrations in soils. However, systematic investigation on their availability in regional soils under industrial impacts is lacking. In this study, 230 paired soil-rice samples were collected from two areas in Southeast China, with low and high industrial impacts. Classic equilibrium-based CaCl2 and EDTA extraction methods, and dynamic-based diffusive gradients in thin-films (DGT) technique were used to study metal availability in soils, with the results being compared with metal concentrations in soils and rice grains. Generally, Cd, Ni, Cu, Zn, Cr and Pb concentrations in soils exceeded the Chinese Soil Quality Standard (GB15618-2018), whereas only Cd and Ni in some rice grains exceeded the Chinese Safety Guidelines. CaCl2 and EDTA extractions, DGT method and soil total metal concentrations provided good predication of grain Cd (R = 0.51-0.66, p < 0.01), whereas only CaCl2 and DGT tests provided good predication of grain Ni (R = 0.36-0.47, p < 0.01). Overall, CaCl2 extraction best predicted Cd and Ni accumulation in rice grains, explaining 66% of grain Cd and 47% of grain Ni. The extraction rate of available Cd was higher than that of Ni, indicating higher Cd availability than Ni, consistent with the parameters (response time, Tc, and desorption rate, k-1) from DIFS (DGT-induced flux in soils) model and bioconcentration factor values. This study showed that, at regional scale, CaCl2 extraction method is efficient in predicting Cd and Ni accumulation in rice grains from contaminated soils.

17.
Am J Nephrol ; 51(1): 65-73, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31770758

RESUMO

BACKGROUND: Successful parathyroidectomy (PTX) with stable postoperative parathyroid hormone (PTH) levels is associated with the stabilization of coronary artery calcification score (CACS) in patients with severe secondary hyperparathyroidism (SHPT) undergoing hemodialysis. To use computed tomography (CT) to evaluate the influence of PTX on CACS in asymptomatic patients. METHODS: This study was performed with institutional review board approval. Written consent was obtained from each patient. Forty-eight patients (mean age ± SD 54.50 ± 13.14 years; range 33-77 years) undergoing maintenance hemodialysis who underwent PTX and 56 patients (mean age 55.28 ± 12.32 years; range 35-76 years) who did not undergo PTX received prospective follow-up for 2 years. CACS was quantitatively performed using the Agatston method after CT scan. The differences between baseline and end-of-study PTX parameters were analyzed with the paired Student t test. RESULTS: For patients who underwent PTX, mean square root of CACS did not increase from 23.16 ± 18.58 to 23.68 ± 18.16 (t = 1.846, p = 0.072) from baseline to follow-up. In patients who did not undergo PTX, mean square root of CACS increased from 20.21 ± 16.47 to 28.72 ± 20.95 (t = 10.110, p < 0.001) from baseline to follow-up. In addition, the median annual progression rate of CACS was lower among PTX patients (4.89%), compared with the marked annual progression rate of CACS in non-PTX patients (52.81%; t = 11.264, p < 0.001). CONCLUSION: Dysregulation of calcium and phosphate metabolism caused by SHPT may result in significant CAC progression in asymptomatic hemodialysis patients. Low PTH state after PTX may stabilize CAC progression in hemodialysis patients.

18.
World Neurosurg ; 134: e8-e11, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31421295

RESUMO

BACKGROUND: Hypothyroidism is widely thought to cause vascular endothelial disorders and atherosclerosis. The purpose of this study was to explore whether patients with hypertension and hypothyroidism may have a higher incidence of hypertensive intracerebral hemorrhage. METHODS: Cases of hypertensive intracerebral hemorrhage collected from the neurology department and neurosurgery department of our hospital from January 1, 2018, to December 31, 2018, were retrospectively collected. A case-control study was conducted on an equal number of patients with hypertension without hypertensive intracerebral hemorrhage randomly selected through age matching in the same period. The history of hypothyroidism and other common risk factors at admission was recorded. RESULTS: A total of 231 patients with hypertensive intracerebral hemorrhage were included and 231 patients with hypertension were selected for control subjects according to the age matching and random screening principles. Hypothyroidism was present in 54 patients (23.4%) and 33 matched controls (14.3%). Multivariate logistic regression analysis showed that hypothyroidism was an independent risk factor for hypertensive intracerebral hemorrhage (odds ratio, 2.29; 95% confidence interval, 1.38-3.79; P = 0.001). CONCLUSIONS: Hypothyroidism may be independently associated with hypertensive intracerebral hemorrhage. In view of the known pathophysiologic relationship between hypothyroidism and vascular endothelial dysfunction and atherosclerosis, further research and exploration are necessary.

19.
J Eukaryot Microbiol ; 67(1): 45-53, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31332864

RESUMO

Nosema bombycis (Nb) is a deadly species of microsporidia capable of causing pébrine, leading to heavy losses in sericulture. Germination is an important biological event in the invasion process of microsporidia. Septins, a family of membrane-associated proteins, play a critical role in tissue invasion and have been recognized as a virulence factor in numerous pathogens. Previous work in our laboratory has shown that Nosema bombycis septin2 (Nbseptin2) interacts with subtilisin-like protease 2 (NbSLP2). Herein, we found that Nbseptin2 was mainly associated with the plasma membrane in spores. Following spore germination, Nbseptin2 was found to co-localize with polar tube protein 1 (NbPTP1) at the polar cap and proximal zone of the polar tube. Co-immunoprecipitation and yeast two-hybrid analysis further confirmed that Nbseptin2 interacted with NbPTP1. The translocation and interaction of Nbseptin2 in the spores suggest that Nbseptin2 may play a significant role in microsporidia polar tube extrusion process. Our findings improve understanding of the mechanisms underlying microsporidia germination.

20.
Protoplasma ; 257(2): 537-547, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31811389

RESUMO

Energy dissipation plays a crucial role in mediating responses to oxidative stress in plants. Although the beneficial effects of silicon on plant resistance to drought stress have been well documented, the potential interactions between energy dissipation and Si in response to drought stress have not been examined. Here, a project was initiated that focused on the relationship between energy dissipation and the functions of Si. In this study, silicon-mediated proteins promoted the consumption of light energy capture and NPQ in chloroplasts. Additionally, we confirmed that the role of silicon-mediated energy dissipation in mitochondria was important for photosynthetic optimization. The energy dissipation in mitochondria was improved, which further optimized the energy dissipation in chloroplasts via Si-mediated alternative oxidase and the malate/oxaloacetate shuttle. ROS accumulation decreased because of the silicon-mediated energy dissipation.

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