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Neural Regen Res ; 16(2): 388-393, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32859803


Massage therapy is an alternative treatment for chronic pain that is potentially related to brain plasticity. However, the underlying mechanism remains unclear. We established a peripheral nerve injury model in rats by unilateral sciatic nerve transection and direct anastomosis. The experimental rats were treated over the gastrocnemius muscle of the affected hindlimb with a customized massage instrument (0.45 N, 120 times/min, 10 minutes daily, for 4 successive weeks). Resting-state functional magnetic resonance imaging revealed that compared with control rats, the amplitude of low-frequency fluctuations in the sensorimotor cortex contralateral to the affected limb was significantly lower after sciatic nerve transection. However, amplitudes were significantly higher in the massage group than in a sham-massage group. These findings suggest that massage therapy facilitated adaptive change in the somatosensory cortex that led to the recovery of peripheral nerve injury and repair. This study was approved by the Animal Ethics Committee of Shanghai University of Traditional Chinese Medicine of China (approval No. 201701001) on January 12, 2017.

Brain Behav ; 10(9): e01747, 2020 09.
Artigo em Inglês | MEDLINE | ID: mdl-32657022


INTRODUCTION: Numerous treatments suggest that brain plasticity changes after peripheral nerve injury (PNI), and most studies examining functional magnetic resonance imaging focused on abnormal changes in specific brain regions. However, it is the large-scale interaction of neuronal networks instead of isolated brain regions contributed to the functional recovery after PNI. In the present study, we examined the intra- and internetworks alterations between the related functional resting-state networks (RSNs) in a sciatic nerve injury rat model. METHODS: Ninety-six female rats were divided into a control and model group. Unilateral sciatic nerve transection and direct anastomosis were performed in the latter group. We used an independent component analysis (ICA) algorithm to observe the changes in RSNs and assessed functional connectivity between different networks using the functional networks connectivity (FNC) toolbox. RESULTS: Six RSNs related to PNI were identified, including the basal ganglia network (BGN), sensorimotor network (SMN), salience network (SN), interoceptive network (IN), cerebellar network (CN), and default mode network (DMN). The model group showed significant changes in whole-brain FC changes within these resting-state networks (RSNs), but four of these RSNs exhibited a conspicuous decrease. The interalterations performed that significantly decreased FNC existed between the BGN and SMN, BGN and IN, and BGN and DMN (p < .05, corrected). A significant increase in FNC existed between DMN and CN and between CN and SN (p < .05, corrected). CONCLUSION: The results showed the large-scale functional reorganization at the network level after PNI. This evidence reveals new implications to the pathophysiological mechanisms in brain plasticity of PNI.

Mapeamento Encefálico , Traumatismos dos Nervos Periféricos , Animais , Encéfalo/diagnóstico por imagem , Feminino , Imageamento por Ressonância Magnética , Rede Nervosa/diagnóstico por imagem , Plasticidade Neuronal , Traumatismos dos Nervos Periféricos/diagnóstico por imagem , Ratos
Brain Res ; 1690: 61-73, 2018 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-29654733


Electroacupuncture (EA) is an alternative therapy for peripheral nerve injury (PNI). The treatment relies on post-therapeutic effect rather than real-time effect. We utilized fMRI to clarify the resting-state alteration caused by sustained effect of EA on peripheral nerve repairing model. Twenty-four rats were divided equally into three groups: normal group, model group and intervention group. Rats of the model and intervention group underwent sciatic nerve transection and direct anastomosis. EA intervention at ST-36 and GB-30 was conducted continuously for 4 months on the intervention group. Behavioral assessments and fMRI were performed 1 month and 4 months after surgery. Intervention group showed significant improvement on the gait parameters max contact mean intensity (MCMI) and thermal withdrawal latency (TWL) than model group. EA-related sustained effects of amplitude of low frequency fluctuations (ALFF) could be described as a remolding pattern of somatosensory area and sensorimotor integration regions which presented higher ALFF in the contralateral hemisphere and lower in the ipsilateral hemisphere than model group. Interhemispheric functional connectivity (FC) analysis showed a significantly lower FC after EA therapy between the largest significantly different clusters in bilateral somatosensory cortices than the model group 4 months after surgery(p < 0.05). And the model group presented significantly higher FC than the normal group at both two time-points (p < 0.01). The sustained effect of EA on peripheral nerve repairing rats appeared to induce both regional and extensive neuroplasticity in bilateral hemispheres. We proposed that such EA-related effect was a reverse of maladaptive plasticity caused by PNI.

Encéfalo/fisiopatologia , Eletroacupuntura , Traumatismos dos Nervos Periféricos/fisiopatologia , Traumatismos dos Nervos Periféricos/terapia , Nervo Isquiático/lesões , Animais , Encéfalo/diagnóstico por imagem , Mapeamento Encefálico , Modelos Animais de Doenças , Imageamento por Ressonância Magnética , Masculino , Condução Nervosa , Vias Neurais/diagnóstico por imagem , Vias Neurais/fisiopatologia , Plasticidade Neuronal , Limiar da Dor , Traumatismos dos Nervos Periféricos/diagnóstico por imagem , Ratos Sprague-Dawley , Descanso , Nervo Isquiático/fisiopatologia
World Neurosurg ; 114: e267-e282, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29524702


OBJECTIVE: We used functional magnetic resonance imaging to provide a longitudinal description of cortical plasticity caused by electroacupuncture (EA) of sciatic nerve transection and direct anastomosis in rats. METHODS: Sixteen rats in a sciatic nerve transection and direct anastomosis model were randomly divided into intervention and control groups. EA intervention in the position of ST-36, GB-30 was conducted continuously for 4 months in the intervention group. Functional magnetic resonance imaging and gait assessment were performed every month after intervention. RESULTS: The somatosensory area was more activated in the first 2 months and then deactivated in the rest 2 months when EA was applied. The pain-related areas had the same activation pattern as the somatosensory area. The limbic/paralimbic areas fluctuated more during the EA intervention, which was not constantly activated or deactivated as previous studies reported. We attributed such changes in somatosensory and pain-related areas to the gradual reduction of sensory afferentation. The alterations in limbic/paralimbic system might be associated with the confrontation between the upregulating effect of paresthesia or pain and the downregulating effect of EA intervention through the autonomic nerve system. The gait analysis showed significantly higher maximum contact mean intensity in the intervention group. CONCLUSIONS: The alterations in the brain brought about by the long-term therapeutic effect of EA could be described as a synchronized activation pattern in the somatosensory and pain-related areas and a fluctuating pattern in the limbic/paralimbic system.

Anastomose Cirúrgica/métodos , Mapeamento Encefálico , Córtex Cerebral/fisiopatologia , Eletroacupuntura/métodos , Neuropatia Ciática/terapia , Animais , Córtex Cerebral/diagnóstico por imagem , Modelos Animais de Doenças , Lateralidade Funcional , Transtornos Neurológicos da Marcha/etiologia , Processamento de Imagem Assistida por Computador , Estudos Longitudinais , Imageamento por Ressonância Magnética , Masculino , Oxigênio/sangue , Ratos , Ratos Sprague-Dawley , Neuropatia Ciática/complicações , Neuropatia Ciática/patologia
Ying Yong Sheng Tai Xue Bao ; 25(12): 3521-6, 2014 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-25876403


Pot grown cotton plants were watered with saltwater (NaCl solutions of different concentrations), followed by a duration of progressive drought stress. The changes in plantlet growth, photosynthetic rate, chlorophyll fluorescence were measured, and the water status of the plantlets, such as relative water content, water potential, osmotic potential, the Na+ and K+ contents in leaves during drought were measured and analyzed, in order to get an insight into the role of Na+ played in the adaptation of cotton to drought stress. The results showed that the growth of the plantlets was significantly inhibited, the net photosynthetic rates were remarkably lowered by the drought stress, but the plant height, biomass, net photosynthetic rate and Fv/Fm values in the cotton plants watered with 25-100 mmol x L(-1) x NaCl solution under drought stress were significantly higher than those watered with water under the same intensity of drought stress. Meanwhile, the soil and leaf relative water content, cell turgor, Na+ concentration in plants watered with 25-100 mmol x L(-1) NaCl solution were all significantly higher compared with that of plants watered with water, but the plant water potential and tissue osmotic potential were significantly lower with the decrease in tissue osmotic potential significantly correlated with the Na+ content. These results indicated that the presence of a moderate amount of Na+ in the soil could improve the water status of both the soil and the cotton plants, accelerate the absorption and accumulation of Na+ in the roots, lower the tissue osmotic potential, thereby enhancing the suction force of water in plant for maintaining a high cell turgor to maintain a relatively higher photosynthetic rate and growth rate. In this sense, it was suggested that the existence of a certain amount of NaCl in soil could effectively alleviate the adverse effects of drought on cotton.

Irrigação Agrícola , Secas , Gossypium/crescimento & desenvolvimento , Fotossíntese , Adaptação Fisiológica , Biomassa , Folhas de Planta/química , Raízes de Plantas/química , Plântula/crescimento & desenvolvimento , Sódio/química , Cloreto de Sódio/química , Solo/química , Água