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1.
Zhonghua Fu Chan Ke Za Zhi ; 56(7): 451-457, 2021 Jul 25.
Artigo em Chinês | MEDLINE | ID: mdl-34304436

RESUMO

Objective: To investigate the incidence and trend of severe postpartum hemorrhage (sPPH) in China, and to provide basic data for the development and evaluation of sPPH prevention and control strategy. Methods: Obstetric data was extracted from annual national representative sampling surveys based on the National Clinical Improvement System. From 2016 to 2019, 2 978, 3 400, 4 576 and 4 594 maternity hospitals with sPPH cases were included for statistics. The annual incidence of sPPH was calculated according to province and type of medical institutions and generalized linear model was emplyed to identify the determinants affecting sPPH incidence. Results: In China, sPPH incidence increased from 0.62% in 2016 to 0.93% in 2018, and was 0.92% in 2019. Eighteen provinces had an inverted U-shaped trend of sPPH over time and most of them had the highest incidence in 2018; ten provinces had an upward trend of sPPH and 3 provinces had a U-shaped trend. In 2019, the top five provinces with the highest sPPH incidence were Yunnan (1.88%), Beijing (1.45%), Jiangsu (1.31%), Guizhou (1.26%), and Ningxia Hui Autonomous Region (1.22%); the top five provinces with the lowest incidence were Henan (0.55%), Jiangxi (0.60%), Inner Mongolia Autonomous Region (0.64%), Liaoning (0.64%) and Gansu (0.69%). In 2019, the sPPH incidence in different types of medical institutions were as follows: tertiary public general hospital (1.15%), tertiary public specialized hospital (1.02%), secondary public general hospital (0.81%), private hospital (0.61%) and secondary public specialized hospital (0.58%). sPPH incidence was positively associated with proportion of twin pregnancies, macrosomia, primipara, and puerpera aged ≥35 years in maternity hospitals (P<0.05). Conclusions: sPPH incidence generally showes an increasing trend from 2016 and is stable at a high level in recent two years in China. It is warranted to further strengthen the monitoring of postpartum hemorrhage, and improve the capability of hierarchical management and treatment in maternity institutions and regions, in order to reduce sPPH incidence and maternal mortality.


Assuntos
Hemorragia Pós-Parto , Pequim , China/epidemiologia , Feminino , Humanos , Incidência , Hemorragia Pós-Parto/epidemiologia , Período Pós-Parto , Gravidez
3.
J Dairy Sci ; 102(3): 2443-2452, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30612791

RESUMO

Escherichia coli is a cause of subclinical and clinical mastitis in dairy cattle and goats, and sometimes causes severe clinical disease that may result in death of the animal. Previous investigation showed that ginsenoside Rg1 extracted from Panax ginseng C.A. Meyer (Araliaceae) has an anti-inflammatory effect on the sepsis induced by E. coli lipopolysaccharide via competitive binding to toll-like receptor 4. We hypothesized that intravenous injection of Rg1 had therapeutic effect on mastitis experimentally induced by intramammary infusion of lipopolysaccharide in lactating goats. In this study, 9 lactating goats were randomly assigned to 1 of the 3 groups: (1) lipopolysaccharide intramammary infusion + saline intravenous injection, (2) lipopolysaccharide intramammary infusion + Rg1 intravenous injection, and (3) saline intramammary administration + saline intravenous injection. Because no adverse clinical signs were observed after intramammary infusion of saline and intravenous injection of Rg1 in a preliminary experiment, and available qualified goats were limited in this study, this treatment was not included in this study. One udder half of each goat received intramammary infusion of lipopolysaccharide (50 µg/kg of body weight; groups 1 and 2) or saline solution (group 3), and the other half was infused with 2 mL of saline solution at h 0. Afterward, intravenous injections of saline solution (groups 1 and 3) or Rg1 (2.5 mg/kg of body weight; group 2) were administered at h 2 and 4 post-lipopolysaccharide challenge. Blood and milk samples were collected 3, 6, 9, 12, 15, 18, 21, 24, 48, and 72 h post-lipopolysaccharide challenge, and clinical signs were monitored hourly after lipopolysaccharide challenge within the first 10 h and at the same time points as blood samples. The results showed that Rg1 treatment downregulated rectal temperature, udder skin temperature, udder girth, milk somatic cell count, and N-acetyl-ß-d-glucosaminidase and upregulated milk production, lactose, and recovered blood components, such as white blood cells, neutrophils, lymphocytes, total proteins, albumin, and globulin. Considering the positive therapeutic effect on lipopolysaccharide-induced mastitis in goats presented in this study as well as the anti-inflammatory activity found previously, the botanical Rg1 deserves further study as a therapeutic agent in the treatment of E. coli mastitis in dairy animals.


Assuntos
Anti-Inflamatórios/uso terapêutico , Ginsenosídeos/uso terapêutico , Doenças das Cabras/tratamento farmacológico , Lipopolissacarídeos/farmacologia , Extratos Vegetais/uso terapêutico , Animais , Anti-Inflamatórios/química , Feminino , Ginsenosídeos/química , Doenças das Cabras/imunologia , Cabras , Injeções Intravenosas/veterinária , Panax/química , Extratos Vegetais/química , Distribuição Aleatória
4.
Eur Rev Med Pharmacol Sci ; 22(20): 6896-6904, 2018 10.
Artigo em Inglês | MEDLINE | ID: mdl-30402855

RESUMO

OBJECTIVE: To explore the behavioral changes and the expressions of the A1 receptor (A1R) and balanced nucleoside transporter-1 (ENT1) in the brain of epileptic rats after activating the NF-E2-related factor 2 (Nrf2)-ARE signaling pathway. MATERIALS AND METHODS: Adult male Sprague-Dawley (SD) rats were randomly divided into normal control group, epilepsy group, and t-butylhydroquinone (tBHQ) group, with 10 rats in each group. Lithium-pilocarpine induced epilepsy model in rats was established. The first epileptic latency and seizure frequency within 1 hour were observed in each group using the Racine scoring system. HE (Hematoxylin and Eosin) staining was used to observe the pathological lesions in the brain tissue of each group. The expressions of A1R, ENT1, and relative genes in Nrf2-ARE pathway in rat hippocampus was detected by immunohistochemistry and Western blot. RESULTS: Compared with rats in epileptic group, the first seizure latency was prolonged and the seizure frequency decreased in tBHQ group (p<0.05). The degree of brain lesions in tHBQ group was lighter than that of epilepsy group. ENT1 expression in rat hippocampus of epileptic group was significantly upregulated than that of normal control group and tBHQ group. Besides, the protein levels of A1R, Nrf2, HO-1, and ARE in rat hippocampus of epilepsy group markedly decreased compared with those of normal control group. However, protein expressions of A1R, Nrf2, HO-1, and ARE proteins in rat hippocampus of tBHQ group were markedly upregulated. CONCLUSIONS: Activation of the Nrf2-ARE signaling pathway can reduce the pathological damage of rat hippocampal neurons, prolong the latency of seizures, and reduce the degree of epileptic seizure in rats.


Assuntos
Proteínas de Transporte/genética , Epilepsia/patologia , Fator 2 Relacionado a NF-E2/metabolismo , Receptor A1 de Adenosina/genética , Animais , Encéfalo/metabolismo , Modelos Animais de Doenças , Transportador Equilibrativo 1 de Nucleosídeo , Hipocampo/metabolismo , Hidroquinonas/administração & dosagem , Masculino , Neurônios/metabolismo , Ratos , Ratos Sprague-Dawley , Convulsões/patologia , Transdução de Sinais , Regulação para Cima
5.
Eur Rev Med Pharmacol Sci ; 22(5): 1366-1373, 2018 03.
Artigo em Inglês | MEDLINE | ID: mdl-29565495

RESUMO

OBJECTIVE: Acute lymphocytic leukemia (ALL) is a multi-factorial blood disease with unknown pathogenesis. Histone H3K4 methylation was significantly reduced in ALL patients, whereas jumonji AT-rich interactive domain 1B (JARID1B) was the specific demethylase of H3K4me. This study explores the expression level of JARID1B in ALL patients and down-regulated JARID1B expression in ALL cells to explore the function of JARID1B in ALL. PATIENTS AND METHODS: JARID1B mRNA expression level in ALL patients was detected by Real-time PCR. The peripheral blood mononuclear cells from healthy volunteers were selected as control. JARID1B shRNA was transfected with MOLT-4 cells and BALL-1 cells. JARID1B protein expression and H3K4me2 and H3K4me3 levels were detected by Western blot assay. Cell proliferation was assessed by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay. Cell apoptosis and cell cycle were determined by flow cytometry. Bcl-2, Bax, Procaspase 3, and cyclin P21 expressions were evaluated by Western blot assay. RESULTS: JARID1B mRNA expression in primary bone marrow cells from ALL patients was significantly higher than that of healthy volunteers (p<0.05). The levels of histone H3K4me3 and H3K4me2 were up-regulated after JARID1B shRNA transfection. JARID1B shRNA significantly inhibited the proliferation of MOLT-4 and BALL-1 cells, induced apoptosis, and blocked cell cycle in G0/G1 phase compared with the control group (p<0.05). CONCLUSIONS: JARID1B is highly expressed in ALL. Down-regulating its expression inhibited leukemia cell proliferation, promoted apoptosis, and blocked cell cycle in G0/G1 phase through histone H3K4 methylation. JARID1B is an oncogene in ALL.


Assuntos
Histona Desmetilases com o Domínio Jumonji/metabolismo , Proteínas Nucleares/metabolismo , Leucemia-Linfoma Linfoblástico de Células Precursoras/patologia , Proteínas Repressoras/metabolismo , Adolescente , Adulto , Idoso , Apoptose , Caspase 3/metabolismo , Linhagem Celular Tumoral , Proliferação de Células , Regulação para Baixo , Feminino , Pontos de Checagem da Fase G1 do Ciclo Celular , Histonas/metabolismo , Humanos , Histona Desmetilases com o Domínio Jumonji/genética , Masculino , Pessoa de Meia-Idade , Proteínas Nucleares/genética , Leucemia-Linfoma Linfoblástico de Células Precursoras/metabolismo , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Interferência de RNA , RNA Interferente Pequeno/metabolismo , Proteínas Repressoras/genética , Adulto Jovem
6.
Exp Neurol ; 293: 115-123, 2017 07.
Artigo em Inglês | MEDLINE | ID: mdl-28365474

RESUMO

BACKGROUND: T-cell death-associated gene 8 (TDAG8), a member of the proton-sensitive G-protein-coupled receptor (GPCR) class with an immune-specific expression profile, was recently shown to be expressed in the rat brain; however, its role in ischaemic stroke remains unknown. METHODS: We initially confirmed the time-dependent expression of TDAG8 in rat brain tissue after ischaemic stroke and reperfusion. Further evaluations were performed to increase TDAG8 expression 6h prior to middle cerebral artery occlusion (MCAO) by injecting a specific agonist, BTB09089, into the lateral ventricle to increase TDAG8 expression. Twenty-four hours before MCAO, a specific small interfering RNA (siRNA) was introduced. The infarction volume, neurological deficit score and cleaved caspase-3 and Bcl-2 expression were used to assess the effects of TDAG8 on ischaemic stroke. Finally, the effects of TDAG8 on the development of primary cortical neurons exposed to oxygen-glucose deprivation (OGD) were investigated. RESULTS: TDAG8 expression increased both in vivo and in vitro. Pretreatment with BTB09089 up-regulated TDAG8 and Bcl-2 expression and down-regulated cleaved caspase-3 expression, while the infarction volume was reduced, and neurological deficits were ameliorated 24 and 72h after MCAO. However, the protective effects of TDAG8 were reversed when its level was reduced in TDAG8-deficient rats. More importantly, these findings are consistent with data from neurons subjected to OGD. CONCLUSIONS: TDAG8 plays an important neuroprotective role through inhibition of neuronal apoptosis and alleviation of neurological deficits by activating the Akt signalling pathway in rats.


Assuntos
Isquemia Encefálica/metabolismo , Proteína Oncogênica v-akt/metabolismo , Receptores Acoplados a Proteínas G/metabolismo , Traumatismo por Reperfusão/metabolismo , Transdução de Sinais/fisiologia , Animais , Isquemia Encefálica/complicações , Isquemia Encefálica/tratamento farmacológico , Células Cultivadas , Córtex Cerebral/citologia , Modelos Animais de Doenças , Embrião de Mamíferos , Regulação da Expressão Gênica/efeitos dos fármacos , Regulação da Expressão Gênica/fisiologia , Glucose/deficiência , Hipóxia/tratamento farmacológico , Hipóxia/metabolismo , L-Lactato Desidrogenase/metabolismo , Masculino , Doenças do Sistema Nervoso/tratamento farmacológico , Doenças do Sistema Nervoso/etiologia , Neurônios/efeitos dos fármacos , Fosfopiruvato Hidratase/metabolismo , Piridazinas/farmacologia , Piridazinas/uso terapêutico , RNA Interferente Pequeno/uso terapêutico , Ratos , Ratos Sprague-Dawley , Receptores Acoplados a Proteínas G/genética , Traumatismo por Reperfusão/complicações , Traumatismo por Reperfusão/tratamento farmacológico , Transdução de Sinais/efeitos dos fármacos , Tiadiazinas/farmacologia , Tiadiazinas/uso terapêutico
8.
Genet Mol Res ; 16(2)2017 Apr 13.
Artigo em Inglês | MEDLINE | ID: mdl-28407176

RESUMO

We investigated the differences between the serum proteomic spectral characteristics of acute myeloid leukemia (AML) patients and those of healthy people. We collected peripheral blood serum samples from 62 AML patients and 15 healthy controls. After removing high-abundance proteins, low-abundance serum proteins were separated using two-dimensional gel electrophoresis to identify differences between AML patients and healthy people. We investigated the different protein dots by mass fingerprint analysis, and evaluated the results using the Masort retrieval program provided by the MSDB protein bank. To further investigate the relationship between standard chemotherapy treatment efficacy and differences in protein patterns, we divided 21 patients into two groups (A and B) according to the efficacy of standard chemotherapy. Compared with the healthy cases, the AML patients demonstrated significant abnormal expression in 14 proteins (P < 0.05); α1-trypsin inhibitor (P < 0.01), prealbumin (P < 0.01), apolipoprotein E (P < 0.010), and apolipoprotein A-IV (P < 0.01) expression decreased, whereas haptoglobin HP2 (P < 0.05), serum exogenous lectin (P < 0.05), H factor homologue protein (P < 0.05), and serum amyloid A1 (P < 0.01) expression increased. Further stratified analysis revealed that patients with high serum lectin expression had poor outcomes. The study revealed various proteins with differential expression levels in the peripheral blood of AML patients, and the difference in serum lectin expression is related to the efficiency of standard chemotherapy. Therefore, these proteins are potential diagnosis markers or prognostic indicators of AML.


Assuntos
Biomarcadores Tumorais/sangue , Leucemia Mieloide Aguda/sangue , Proteoma/química , Adulto , Idoso , Estudos de Casos e Controles , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Proteoma/genética , Proteoma/metabolismo
9.
Eur Rev Med Pharmacol Sci ; 21(3): 606-611, 2017 02.
Artigo em Inglês | MEDLINE | ID: mdl-28239805

RESUMO

OBJECTIVE: To observe the efficacy of traditional administration, continuous pump injection, and closed-loop target controlled infusion of cisatracurium to determine the optimal method of drug administration, and to establish the individualized and rational administration of muscle relaxants in elderly patients. PATIENTS AND METHODS: A total of 150 patients who underwent spinal surgery under tracheal intubation general anesthesia in our hospital from August 2013 to April 2015 were selected. All patients were administered with general anesthesia and randomly divided into three groups: group A (n = 50) was treated under closed-loop target controlled infusion (CLTCI), group B (n = 50) was treated under muscle relaxation monitoring, and group C (n = 50) was treated under continuous pump injection. Hemodynamic changes and blood oxygen saturation of the three groups were observed, and the muscle relaxation recovery, dosage, and bleeding of the three groups were compared. RESULTS: MAP and HR of group A were significantly lower than those of group B and group C (p < 0.05). There were no cases of insufficient muscle relaxation in group A, five cases in group C, and 14 cases in group B, and the differences between any two groups were statistically significant (p < 0.05). Regarding muscle relaxation recovery, the time (T¬¬1) of recovery from 10%-25% and 25%-75%, and the time from drug withdrawal to recovery to TOFr from 0.7-0.9 of group A were the shortest, followed by group C and group B. The differences between any two groups were statistically significant (p < 0.05). The total dosage of cisatracurium of group A was the least, followed by group C and group B, and differences between any two groups were statistically significant (p < 0.05). Moreover, the bleeding volume of group A (235.2 ± 141.3 ml) was smaller than in group B (353.1 ± 173.8 ml) and group C (316.5 ± 155.2 ml), and differences between the three groups were statistically significant (p < 0.05). CONCLUSIONS: For spinal surgery of elderly patients, closed-loop target controlled infusion of cisatracurium was superior to continuous infusion and intravenous injection. The time of muscle relaxation recovery was shortened, the dosage of cisatracurium was reduced, and the number of cases of insufficient muscle relaxation was reduced.


Assuntos
Atracúrio/análogos & derivados , Relaxamento Muscular/efeitos dos fármacos , Coluna Vertebral/cirurgia , Idoso , Anestesia Geral , Atracúrio/administração & dosagem , Feminino , Humanos , Injeções Intravenosas , Masculino , Pessoa de Meia-Idade , Bloqueadores Neuromusculares
10.
Rev Sci Instrum ; 87(11): 11E112, 2016 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-27910414

RESUMO

To meet experimental requirements, the J-TEXT electron cyclotron emission (ECE) diagnostic is being upgraded. The front end antenna and transmission line have been modified and a new 8-channel W-band detecting unit has been developed. The improved ECE system will extend the frequency range from 94.5-124.5 GHz to 80.5-124.5 GHz. This will enable the system to cover the most plasma in the radius direction for BT = 1.8-2.2 T, and it even can cover almost the whole plasma range ρ = - 0.8-0.9 (minus means the high field side) at BT = 1.8 T. A new auxiliary channel bank with 8 narrow band, tunable yttrium iron garnet filters is planned to add to the ECE system. Due to observations along a major radius, perpendicular to BT, and relatively low electron temperature, Doppler and relativistic broadening are minimal and thus high spatial resolution measurements can be made at variable locations with these tunable channels.

11.
Rev Sci Instrum ; 87(11): 11E106, 2016 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-27910430

RESUMO

A new 2D Electron Cyclotron Emission Imaging (ECEI) diagnostic is being developed for the J-TEXT tokamak. It will provide the 2D electron temperature information with high spatial, temporal, and temperature resolution. The new ECEI instrument is being designed to support fundamental physics investigations on J-TEXT including MHD, disruption prediction, and energy transport. The diagnostic contains two dual dipole antenna arrays corresponding to F band (90-140 GHz) and W band (75-110 GHz), respectively, and comprises a total of 256 channels. The system can observe the same magnetic surface at both the high field side and low field side simultaneously. An advanced optical system has been designed which permits the two arrays to focus on a wide continuous region or two radially separate regions with high imaging spatial resolution. It also incorporates excellent field curvature correction with field curvature adjustment lenses. An overview of the diagnostic and the technical progress including the new remote control technique are presented.

12.
Scand J Immunol ; 84(3): 165-73, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27384769

RESUMO

The immune system can be damaged by chronic stress. However, for this process, the involved molecular alterations and their regulatory roles played in immunosuppression still remain unclear. This study was aimed to identify the differences in serum protein expressions that are closely associated with the effect of chronic stress on immune function. Serum protein levels of rats in control group and chronic stress group were measured by iTRAQ analysis. Subsequently, among the 121 differentially expressed proteins screened between the two groups, 46 proteins were upregulated (>1.5-fold, P < 0.05), while 75 proteins were downregulated (<0.67-fold, P < 0.05). Bioinformatics analysis revealed that most of the differentially expressed proteins were in relation with the metabolic, cellular, response stimulus and immune system processes. The significantly differential expression of ceruloplasmin, haptoglobin, catalase and peroxiredoxin-1 were picked out for reconfirmation by ELISA analysis. The results were consistent with those obtained by iTRAQ. What is more, the roles of above-mentioned four proteins, apolipoprotein B-100 and heat-shock protein 90 in immunosuppression induced by chronic stress were discussed. Taken together, these findings may provide a new insight into better understanding the molecular mechanisms of immunosuppression induced by chronic stress.


Assuntos
Regulação da Expressão Gênica/imunologia , Estresse Psicológico/genética , Animais , Apolipoproteína B-100/sangue , Apolipoproteína B-100/genética , Apolipoproteína B-100/imunologia , Catalase/sangue , Catalase/genética , Catalase/imunologia , Ceruloplasmina/genética , Ceruloplasmina/imunologia , Biologia Computacional/métodos , Perfilação da Expressão Gênica , Proteínas de Choque Térmico HSP90/sangue , Proteínas de Choque Térmico HSP90/genética , Proteínas de Choque Térmico HSP90/imunologia , Haptoglobinas/genética , Haptoglobinas/imunologia , Imobilização , Células Matadoras Naturais/química , Células Matadoras Naturais/imunologia , Peroxirredoxinas/sangue , Peroxirredoxinas/genética , Peroxirredoxinas/imunologia , Ratos , Ratos Wistar , Estresse Psicológico/imunologia , Natação
13.
Rev Sci Instrum ; 86(4): 043501, 2015 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-25933856

RESUMO

To study the anomalous transport, a correlation electron cyclotron emission (CECE) was planned to be developed on J-TEXT for electron temperature fluctuation measurement. The spectral decorrelation method was employed for the CECE system. It was developed based on the previous 16-channel electron cyclotron emission system. They shared the optical transmission line and mixer. The CECE part consists of 4 channels. Two fixed frequency narrow band filters were used for two channels and two yttrium iron garnet (YIG) filters for the other two channels. To meet the measuring requirement, some tests have been taken for the YIG filters. The results show good performance of the filters. Gaussian optics is used to produce a good poloidal resolution. Wavenumbers resolved by the CECE diagnostic are k(θ) ≤ 1.5 rad/cm and k(r) ≤ 12 rad/cm. Some preliminary experiment results are also presented in this paper.

14.
Eur Rev Med Pharmacol Sci ; 18(20): 3045-7, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-25392103

RESUMO

OBJECTIVE: To explore surgical strategies for unexpected gallbladder carcinoma (UGC). PATIENTS AND METHODS: A study of hospital records was performed in 26 patients with UGC treated in our institution from January 1990 to December 2002. The surgical therapies and disease prognosis were analyzed. RESULTS: Five cases of GBC were noted during open cholecystectomy (OC) and 15 were confirmed during minicholecystectomy (MC), while were 6 identified during laparoscopic (LC) surgery; all patients with Nevin Stage I gallbladder carcinoma (GBC) survived > 3 years; patients with Stage III GBC died within 2 years and patients with Stage IV GBC died within 1 year. CONCLUSIONS: Caution must be taken when high risk conditions and atypical ultrasonography of gallbladder are encountered. Examination of frozen sections should be performed routinely in all cases to reduce the rates of misdiagnosis. Surgery is an effective treatment for GBC.


Assuntos
Colecistectomia Laparoscópica/métodos , Neoplasias da Vesícula Biliar/diagnóstico por imagem , Neoplasias da Vesícula Biliar/cirurgia , Adulto , Idoso , Idoso de 80 Anos ou mais , Colecistectomia/métodos , Erros de Diagnóstico/prevenção & controle , Feminino , Secções Congeladas/métodos , Neoplasias da Vesícula Biliar/mortalidade , Humanos , Masculino , Pessoa de Meia-Idade , Prognóstico , Resultado do Tratamento , Ultrassonografia
15.
Gene ; 524(2): 208-13, 2013 Jul 25.
Artigo em Inglês | MEDLINE | ID: mdl-23644138

RESUMO

BRIP1 (BRCA1-interacting protein 1), a DNA-dependent ATPase and a DNA helicase, is critical for BRCA-associated DNA damage repair functions, and may be involved in the development of cervical cancer. Genetic markers in different regions of the BRIP1 gene have a plausible role in modulating the risk of cervical cancer. In this study, we evaluate the association between the BRIP1 variations and the risk of cervix cancer. We examined the potential association between cervical cancer and eighteen single nucleotide polymorphisms (SNPs, rs2048718, rs16945692, rs4968451, rs6504074, rs4988344, rs8077088, rs10515211, rs9897121, rs9906313, rs2159450, rs4986764, rs11871785, rs4986763, rs11079454, rs7213430, rs34289250, rs4988345 and rs12937080) of the BRIP1 gene using the MassARRAY system. The participants enrolled in this study included 298 patients with cervical cancer and 286 healthy women as the healthy controls from a Chinese Han population. The results showed that rs16945692 (intron 1), rs4968451 (intron 4), rs4986764 (exon 18) and rs7213430 (3'UTR) were significantly associated with cervical cancer (P<0.05). Furthermore, strong linkage disequilibrium (LD) was observed in three blocks (D'>0.9), and significantly more T-A-C-A haplotypes (block 1) (P=0.001) were found in the patients with cervical cancer. Significantly higher frequencies of C-A-T haplotypes (block 2) (P=0.018) and A-A haplotypes (block 3) (P=0.009) were detected in the healthy controls than in the patients with cervical cancer, suggesting that they may show protective effects against cervical cancer. These findings point to a role for the BRIP1 gene polymorphisms in cervical cancer in a Chinese Han population, and may be informative for future genetic or biological studies on cervical cancer.


Assuntos
Proteínas de Ligação a DNA/genética , Predisposição Genética para Doença , Polimorfismo de Nucleotídeo Único , RNA Helicases/genética , Neoplasias do Colo do Útero/genética , Adulto , Estudos de Casos e Controles , China/epidemiologia , Éxons , Proteínas de Grupos de Complementação da Anemia de Fanconi , Feminino , Frequência do Gene , Estudos de Associação Genética , Haplótipos , Humanos , Íntrons , Desequilíbrio de Ligação , Pessoa de Meia-Idade , Neoplasias do Colo do Útero/enzimologia , Neoplasias do Colo do Útero/etnologia
16.
Biochem Biophys Res Commun ; 433(2): 232-6, 2013 Apr 05.
Artigo em Inglês | MEDLINE | ID: mdl-23473757

RESUMO

To evaluate the association between gene variations in BRIP1 (BRCA1-interacting protein 1) and the risk of cervical cancer, we examined eight single nucleotide polymorphisms (SNPs: rs2048718, rs12937080, rs4988344, rs6504074, rs4988345, rs4986764, rs4986763, and rs11079454) in the BRIP1 gene in cervical tissue from a Chinese population using the MassARRAY system. The participants enrolled included 454 cervical cancer patients and 562 healthy controls. Quantitative real-time reverse transcription PCR (qRT-PCR) was performed to examine the potential correlation between functional BRIP1 SNP genotypes and mRNA levels in cervical cancer tissues. Our results first showed that rs4986764, located in exon 18 in the BRIP1 gene, was significantly associated with cervical cancer (χ(2)=11.191, P=0.001, odds ratio (OR)=1.384, 95% confidence interval (CI)=1.144-1.675). Another significant association was observed for rs4986763 located in exon 20 in BRIP1 (χ(2)=4.988, P=0.026, OR=1.241, 95% CI=1.027-1.500). Strong linkage disequilibrium was observed in the rs11079454-rs4986763-rs4986764 SNP block (D'>0.9). The frequencies of haplotype T-T-T are higher in controls than in these patients (P=2.01E-5). Moreover, cervical cancer tissues with a homozygous C/C genotype for rs4986764 had the lowest level of BRIP1, which was 2.8 and 2.9-fold lower than the C/T heterozygote and the T/T homozygote, respectively. These findings indicate a role for BRIP1 gene variations in cervical cancer and may be informative for future genetic or biological studies on cervical cancer.


Assuntos
Proteínas de Ligação a DNA/genética , Predisposição Genética para Doença , Polimorfismo de Nucleotídeo Único , RNA Helicases/genética , Neoplasias do Colo do Útero/genética , Adulto , Estudos de Casos e Controles , Proteínas de Grupos de Complementação da Anemia de Fanconi , Feminino , Homozigoto , Humanos , Desequilíbrio de Ligação , Pessoa de Meia-Idade , RNA Mensageiro/análise , RNA Mensageiro/metabolismo
17.
Scott Med J ; 56(4): 214-9, 2011 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-22089043

RESUMO

Our aim was to explore the clinical application value of high-intensity focused ultrasound (HIFU) therapy for tubal pregnancy. Forty hospitalized patients with tubal pregnancies (28 cases of non-ruptured tubal pregnancy and 12 cases of ruptured tubal pregnancy) were selected to receive HIFU therapy. Serum human chorionic gonadotropin (ß-HCG) concentrations were compared before and after treatment. Serum ß-HCG was measured weekly and patients received observation only if the concentration decreased by 15% or more, compared with the previous value. Patients were given supplement HIFU therapy if the decrease in the serum ß-HCG was <15% within two weeks. Ultrasound was used to detect the volume changes in the ectopic lesions before and after treatment, and changes in vital signs and complications were recorded. Contrast-enhanced ultrasonography was used to assess fallopian tube patency after treatment. HIFU treatment was successful in 33 of the 40 patients (82%). Seven patients failed HIFU treatment and received surgical therapy (18%). Before and after treatment, serum ß-HCG concentrations and lesion volume were significantly different (P < 0.05, P < 0.01, respectively). Post-treatment tubal contrast-enhanced ultrasonography showed tubal patency on the affected side in 21 cases (64%) at six months and in 27 cases (82%) at 12 months. In conclusion, HIFU is safe and effective, and can be a treatment option for tubal pregnancy.


Assuntos
Ablação por Ultrassom Focalizado de Alta Intensidade , Gravidez Tubária/terapia , Adulto , Gonadotropina Coriônica Humana Subunidade beta/sangue , Testes de Obstrução das Tubas Uterinas , Tubas Uterinas/diagnóstico por imagem , Feminino , Humanos , Gravidez , Gravidez Tubária/sangue , Gravidez Tubária/diagnóstico por imagem , Resultado do Tratamento , Ultrassonografia
18.
Phys Rev Lett ; 102(20): 205503, 2009 May 22.
Artigo em Inglês | MEDLINE | ID: mdl-19519040

RESUMO

A self-corrugated Cu(3)N-Cu(110) molecular network shows the potential to overcome the element dependence barrier as demonstrated by epitaxial growth of atomic nanowires (approximately 1 nm in width) among various 3d, 4d, and 5d elements. Scanning tunneling microscopy shows that all of the investigated atomic nanowires share an identical structure, featuring uniform width, height, orientation and the same minimum separation distance. Ab initio study reveals that the formation mechanism of atomic nanowires can be directly attributed to a strain relief guided asymmetric occupation of atoms on the originally symmetric crest zone of the corrugated network.

19.
Phys Rev Lett ; 102(20): 207206, 2009 May 22.
Artigo em Inglês | MEDLINE | ID: mdl-19519071

RESUMO

The magnetic anisotropy of Fe film grown on vicinal Ag(1,1,10) surfaces was studied with the in situ magneto-optic Kerr effect. Below 200 K, strong oscillations of the uniaxial magnetic anisotropy as a function of Fe thickness with a period of 5.7 monolayers are found, which can even cause the easy magnetization axis to oscillate between perpendicular and parallel to the steps. Such novel oscillation of the anisotropy is attributed to the quantum well states of d-band electrons at the Fermi level in the Fe film. This is unlike the previously observed oscillatory behaviors of ferromagnetic films caused by the quantum well states in nonmagnetic interfacing layers.

20.
Radiat Prot Dosimetry ; 128(1): 68-71, 2008.
Artigo em Inglês | MEDLINE | ID: mdl-17545664

RESUMO

Soft X-rays at carbon, nitrogen, oxygen K-shell edges have special radiobiological effects. Using Aspergillus oryzae spores as sample, the radiation effects of soft X-rays near the K-shell edges of C, N and O elements from synchrotron radiation were investigated. Also the dose depositions of different X-ray energies in spore were discussed. At the same time, the spores were irradiated by gamma rays from 60Co and relative biological effects were compared with those produced by soft X-rays. The results showed that soft X-rays near K-shell edges of O element had higher ability of radiation damage than that of X-rays near K-shell edges of C and N elements as compared with one another. But they all had higher killing abilities per unit dose than that of gamma rays from 60Co. The relative biological effects (RBEs), the comparison of dose to gamma rays at 10% survival level, of the three soft X-rays were 1.65, 1.73 and 1.91, respectively.


Assuntos
Aspergillus oryzae/efeitos da radiação , Esporos/efeitos da radiação , Carbono/química , Radioisótopos de Cobalto , Raios gama , Nitrogênio/química , Oxigênio/química , Raios X
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