Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 13 de 13
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Inflammation ; 2019 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-31802382

RESUMO

Ellipticine, a natural product from Ochrosia elliptica, has been broadly investigated for its anticancer effects. Although inflammation has been clearly identified as a key factor in the onset and progression of cancer, the relationship between ellipticine and inflammation remains unknown. Hence, the aims of the present study were to assess the effects of ellipticine on the inflammatory responses to lipopolysaccharide (LPS)-induced macrophages and to potentially identify the underlying mechanisms involved. Viability testing showed that ellipticine was not significantly toxic to Raw264.7 cells and actually conveyed protective effects to LPS-stimulated Raw264.7 cells and human peripheral blood monocytes by decreasing the secretion of inflammatory factors (TNF-α and IL-6). The results of western blot analysis and electrophoretic mobility shift assays showed that ellipticine markedly suppressed LPS-induced activation of the JNK/AP-1 (c-Fos and c-Jun) signaling pathway, but not ERK/p38/NF-κB pathway (p65 and p50) activation. Furthermore, ellipticine reduced the inflammatory response and mortality in a mouse model of LPS-induced endotoxic shock. Collectively, these data indicate that ellipticine may be a potential therapeutic agent for the treatment of inflammation-associated diseases.

2.
Arch Microbiol ; 200(6): 841-846, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-29846759

RESUMO

Virus infections are the root cause of epidemics in the world. Vaccines and antiviral agents have been the two important methods to control viral diseases; in recent times, RNA-mediated therapeutics and prevention have received much attention. In this review, we provide an overview of the current information regarding the use of vaccines, antiviral agents, and RNA-mediated methods in controlling or preventing viral infections. We stress specifically on the potential of existing RNA-mediated methods in clinical applications.


Assuntos
Antivirais/farmacologia , Descoberta de Drogas/tendências , Viroses/virologia , Vírus/efeitos dos fármacos , Animais , Humanos , RNA Viral/genética , Viroses/tratamento farmacológico , Fenômenos Fisiológicos Virais/efeitos dos fármacos , Vírus/genética
3.
J Vet Res ; 62(4): 431-437, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30729199

RESUMO

Introduction: The extremely high genetic variation and the continuously emerging variants of foot-and-mouth disease virus (FMDV) of Southern African Territory (SAT) serotypes including SAT1, SAT2, and SAT3 make it necessary to develop a new RT-PCR for general use for monitoring viruses based on the updated genome information. Material and Methods: A FMDV SAT-D8 one-step RT-PCR was established based on the 1D2A2B genes of the SAT serotype viruses with a multiplex primer set. FMDV A, O, C, and Asia 1 serotypes, other vesicular disease viruses, inactivated SAT viruses, and 125 bovine, ovine, caprine and porcine tissue samples collected from the Chinese mainland were included for evaluating the assay. Results: The new RT-PCR was proven to be specific without cross-reactions with Eurasian FMDV, swine vesicular disease virus (SVDV), Seneca valley virus (SVV), or other common viral pathogens of cattle, sheep, goat, and pig. An around 257 bp-sized amplicon clearly appeared when the inactivated SAT viruses were detected. However, all 125 samples collected from FMDV-susceptible animals from the Chinese mainland which has not known SAT epidemics showed negative results. Conclusions: A FMDV SAT-D8 one-step RT-PCR is a promising method for primary screening for FMDV SAT serotypes.

4.
Medicine (Baltimore) ; 95(48): e5074, 2016 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-27902584

RESUMO

This study aims to evaluate the effect of naloxone on intravenous fentanyl patient-controlled analgesia after laparoscopic cholecystectomy under total intravenous anesthesia.A total of 90 patients, who underwent intravenous fentanyl patient-controlled analgesia after laparoscopic cholecystectomy under total intravenous anesthesia, were included into this study. All patients were randomly divided into 3 groups (each group, n=30): naloxone group (naloxone+fentanyl), tropisetron group (tropisetron+fentanyl), and fentanyl group (fentanyl). Patients in each group were given a corresponding dose of naloxone. Postoperative analgesia effect and the incidence of side effects such as nausea and vomiting were observed.Small doses of naloxone or tropisetron combined with fentanyl used for intravenous patient-controlled analgesia can significantly reduce the incidence of nausea and vomiting. Six hours after surgery, visual analogue scale (VAS) scores were significantly lower in patients that underwent intravenous patient-controlled analgesia using low-dose naloxone combined with fentanyl compared with patients who received fentanyl alone; however, the postoperative analgesic effect of tropisetron was not observed. Compared with the combination of tropisetron and fentanyl, low-dose naloxone combined with fentanyl can obviously reduce the incidence of nausea and vomiting in patients who underwent intravenous patient-controlled analgesia after laparoscopic cholecystectomy, and enhance the analgesic effect of fentanyl 6 hours after surgery.Low-dose naloxone can reduce the incidence of nausea and vomiting in patients who underwent laparoscopic cholecystectomy under total intravenous anesthesia, and exhibits a certain synergic analgesic effect.


Assuntos
Analgesia Controlada pelo Paciente , Analgésicos Opioides/administração & dosagem , Anestésicos Intravenosos/administração & dosagem , Antieméticos/administração & dosagem , Colecistectomia Laparoscópica , Fentanila/administração & dosagem , Indóis/administração & dosagem , Naloxona/administração & dosagem , Antagonistas de Entorpecentes/administração & dosagem , Dor Pós-Operatória/tratamento farmacológico , Náusea e Vômito Pós-Operatórios/prevenção & controle , Idoso , Quimioterapia Combinada , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Medição da Dor , Tropizetrona
5.
Mil Med Res ; 3: 33, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27833759

RESUMO

Trauma still represents one of the major causes of death worldwide. Despite the reduction of post-traumatic sepsis over the past two decades, the mortality of septic trauma inpatients is still high (19.5-23 %). Early prevention of sepsis development can aid in the subsequent treatment of patients and help improve their outcomes. To date, the prevention of trauma-related infection/sepsis has mainly included infection prevention (e.g., surgical management, prophylactic antibiotics, tetanus vaccination, immunomodulatory interventions) and organ dysfunction prevention (e.g., pharmaceuticals, temporary intravascular shunts, lung-protective strategies, enteral immunonutrition, acupuncture). Overall, more efficient ways should be developed to prevent trauma-related infection/sepsis.


Assuntos
Traumatismo Múltiplo/complicações , Sepse , Infecção dos Ferimentos , Humanos , Sepse/etiologia , Sepse/prevenção & controle , Infecção dos Ferimentos/etiologia , Infecção dos Ferimentos/prevenção & controle
6.
Artigo em Inglês | MEDLINE | ID: mdl-27244702

RESUMO

A new chemosensor L based on the naphthalimide moiety was synthesized and characterized. L exhibited the high selectivity and sensitivity for Al(3+) in CH3OH, along with colorimetric and fluorometric dual-signaling responses based on the joint contribution of the ICT and CHEF processes. A 1:1 stoichiometry for the L-Al(3+) complex was formed with an association constant of 7.6×10(4)M(-1), and the limit of detection for Al(3+) was determined as 6.9µM. In addition, L was successfully applied to the determination of Al(3+) in real water samples.

7.
Guang Pu Xue Yu Guang Pu Fen Xi ; 34(8): 2126-31, 2014 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-25474948

RESUMO

Agarose gel/gold nanoparticles hybrid was prepared by adding gold nanoparticles to preformed agarose gel. Naniocomposite structures and properties were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and UV-Vis-NIR absorption spectroscopy. Experimental data indicated a uniform distribution of gold nanoparticles adsorbed on agarose gel network And the excellent optical absorption properties were shown. Based on the swelling-contraction characteristics of agarose gel and the adjustable localized surface plasmon resonance (LSPR) of the gold nanoparticles, the nano-composites were used as surface enhanced Raman scattering (SERS) substrate to detect the Raman signal molecules Nile blue A. Results revealed that the porous structure of the agarose gel provided a good carrier for the enrichment of the gold nanoparticles. The gold nanoparticles dynamic hot-spot effect arising from the agarose gel contraction loss of water in the air greatly enhanced the Raman signal.


Assuntos
Ouro , Nanopartículas Metálicas , Microscopia Eletrônica de Varredura , Microscopia Eletrônica de Transmissão , Sefarose , Espectroscopia de Luz Próxima ao Infravermelho , Análise Espectral Raman , Ressonância de Plasmônio de Superfície , Propriedades de Superfície
8.
World J Biol Psychiatry ; 14(2): 91-9, 2013 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-21745129

RESUMO

UNLABELLED: Abstract Objectives. Several lines of evidence have shown that both RELN mRNA and protein are possibly down-regulated in the brain of schizophrenia patients. Recent association studies in European populations suggested RELN as a risk gene for schizophrenia. In this study, we test if RELN contributes to the risk of schizophrenia in Chinese population. Methods. We conducted case-control association analysis of 19 representative single nucleotide polymorphisms (SNPs) spanning the entire region of RELN in two independent Han Chinese samples from southwestern China (the Kunming sample and the Yuxi sample). Results. We identified six SNPs significantly associated with schizophrenia in the Kunming sample and four of them remained significant in the combined samples (the P values range from 0.006 to 4.0 × 10(-5)). Haplotype analysis also suggested significant associations for the haplotypes incorporating the six significant SNPs (global P < 1.0 × 10(-5)). Additionally, we also observed several other haplotypes (defined by a different set of SNPs) significantly associated with schizophrenia in the Kunming sample. However, the reported association of rs7341475 in Ashkenazi Jews was not significant in Han Chinese. CONCLUSIONS: Our findings demonstrate that RELN is a susceptibility gene for schizophrenia in Chinese population, and it is likely a common risk gene for schizophrenia in major populations worldwide.


Assuntos
Encéfalo , Moléculas de Adesão Celular Neuronais , Proteínas da Matriz Extracelular , Proteínas do Tecido Nervoso , Esquizofrenia/genética , Serina Endopeptidases , Adulto , Encéfalo/metabolismo , Encéfalo/patologia , Moléculas de Adesão Celular Neuronais/genética , Moléculas de Adesão Celular Neuronais/metabolismo , China , Regulação para Baixo , Proteínas da Matriz Extracelular/genética , Proteínas da Matriz Extracelular/metabolismo , Feminino , Frequência do Gene , Predisposição Genética para Doença , Haplótipos , Humanos , Classificação Internacional de Doenças , Masculino , Pessoa de Meia-Idade , Proteínas do Tecido Nervoso/genética , Proteínas do Tecido Nervoso/metabolismo , Polimorfismo de Nucleotídeo Único , Esquizofrenia/diagnóstico , Esquizofrenia/metabolismo , Serina Endopeptidases/genética , Serina Endopeptidases/metabolismo
10.
Acta Crystallogr C ; 66(Pt 9): m245-8, 2010 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-20814095

RESUMO

The asymmetric unit of the title compound, [Pb(2)(C(8)H(4)O(4))(2)(C(18)H(11)N(5))(2)](n), contains two Pb(II) atoms, two benzene-1,4-dicarboxylate (1,4-bdc) dianions and two 6-(4-pyridyl)-5H-imidazolo[4,5-f][1,10]phenanthroline (L) ligands. Each Pb(II) atom is eight-coordinated by three N atoms from two different L ligands and five carboxylate O atoms from three different 1,4-bdc dianions. The two 1,4-bdc dianions (1,4-bdc(1) and 1,4-bdc(2)) show different coordination modes. Each 1,4-bdc(1) coordinates to two Pb(II) atoms in a chelating bis-bidentate mode. Each carboxylate group of the 1,4-bdc(2) anion connects two Pb(II) atoms in a chelating-bridging tridentate mode to form a dinuclear unit. Neighbouring dinuclear units are connected together by the aromatic backbone of the 1,4-bdc dianions and the L ligands into a three-dimensional six-connected alpha-polonium framework. The most striking feature is that two identical three-dimensional single alpha-polonium nets are interlocked with each other, thus leading directly to the formation of a twofold interpenetrated three-dimensional alpha-polonium architecture. The framework is held together in part by strong N-H...O hydrogen bonds between the imidazole NH groups of the L ligands and the carboxylate O atoms of 1,4-bdc dianions within different alpha-polonium nets.

11.
Acta Crystallogr C ; 65(Pt 12): m459-62, 2009 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-19966426

RESUMO

In the isomorphous title compounds, [Cd(2)(C(8)H(4)O(4))(2)(C(19)H(10)ClFN(4))(2)(H(2)O)(2)] and [Zn(2)(C(8)H(4)O(4))(2)(C(19)H(10)ClFN(4))(2)(H(2)O)(2)], the Cd(II) centre is seven-coordinated by two N atoms from one [2-(2-chloro-6-fluorophenyl)-1H-imidazo[4,5-f][1,10]phenanthroline (L) ligand, one water O atom and four carboxylate O atoms from two different benzene-1,2-dicarboxylate (1,2-bdc) ligands in a distorted pentagonal-bipyramidal coordination, while the Zn(II) centre is six-coordinated by two N atoms from one L ligand, one water O atom and three carboxylate O atoms from two different 1,2-bdc ligands in a distorted octahedral coordination. Each pair of adjacent metal centres is bridged by two 1,2-bdc ligands to form a dimeric structure. In the dimer, each L ligand coordinates one metal centre. The dimer is centrosymmetric, with a crystallographic inversion centre midway between the two metal centres. The aromatic interactions lead the dimers to form a two-dimensional supramolecular architecture. Finally, O-H...O and N-H...O hydrogen bonds reinforce the two-dimensional structures of the two compounds.

12.
Acta Crystallogr C ; 65(Pt 12): m472-4, 2009 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-19966430

RESUMO

The asymmetric unit of the title compound, [Cd(C(8)H(4)O(4))(C(17)H(8)ClN(5))(H(2)O)](n), contains one Cd(II) atom, two half benzene-1,4-dicarboxylate (1,4-bdc) anions, one 11-chloropyrido[2',3':2,3]pyrimidino[5,6-f][1,10]phenanthroline (L) ligand and one coordination water molecule. The 1,4-bdc ligands are on inversion centers at the centroids of the arene rings. The Cd(II) atom is six-coordinated by two N atoms from one L ligand, three carboxylate O atoms from two different 1,4-bdc ligands and one water O atom in a distorted octahedral coordination sphere. Each Cd(II) center is bridged by the 1,4-bdc dianions to give a one-dimensional chain. Pi-pi stacking interactions between L ligands of neighboring chains extend adjacent chains into a two-dimensional supramolecular (6,3) network. Neighboring (6,3) networks are interpenetrated in an unusual inclined mode, resulting in a three-dimensional framework. Additionally, the water-carboxylate O-H...O hydrogen bonds observed in the network consolidate the interpenetrating nets.

13.
Acta Crystallogr C ; 65(Pt 8): m276-8, 2009 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-19652299

RESUMO

In the title coordination compound, [Mn(C8H10O4)(C14H14N4)(H2O)2]n, each Mn(II) centre occupies an inversion centre. The 1,4-bis(imidazol-1-ylmethyl)benzene (1,4-bix) ligand and the trans-cyclohexane-1,4-dicarboxylate dianion (chdc) both function in bridging modes, linking adjacent Mn(II) centres into a two-dimensional four-connected (4,4) network. These two-dimensional layers are stacked in a parallel mode. Hydrogen bonds between water molecules and carboxylate O atoms link neighbouring (4,4) networks, yielding a three-dimensional alpha-polonium net.


Assuntos
Manganês/química , Compostos Organometálicos/química , Cristalografia por Raios X , Ligações de Hidrogênio , Ligantes , Modelos Moleculares , Água
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA