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1.
Analyst ; 2019 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-31710318

RESUMO

This study deals with the nonaqueous capillary electrophoretic enantioseparation of twenty-two amino alcohol drugs with a maltobionic acid (MA)-based ionic liquid (tetramethylammonium maltobionic acid, TMA-MA) as the novel chiral selector. In consideration of the poor solubility of MA in organic solvents, we managed to transform MA into ionic liquids (ILs) for the first time. Interestingly, this chiral selector exhibited powerful enantioselectivity towards the model analytes in company with boric acid. Systematical experiments were carried out to investigate the influence of concentration of TMA-MA, boric acid and tris (hydroxymethyl) aminomethane (Tris) as well as applied voltage on the enantioseparation. A great majority of enantiomers (except labetalol) were baseline separated under the optimized conditions and the effect of the molecular structure of amino alcohol drugs on the chiral separation was discussed. In addition, electrophoretic experiments, nuclear magnetic resonance (NMR), mass spectrometry (MS) and molecular modeling with the Gaussian program were employed to demonstrate the mechanism of chiral recognition. Based on the formation of an ionic liquid-boric acid-analyte complex, hydrogen binding was mainly responsible for enantioseparation.

2.
Heredity (Edinb) ; 2019 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-31527784

RESUMO

Flowering time is one of the most critical traits for plants' life cycles, which is influenced by various environment changes, such as global warming. Previous studies have suggested that to guarantee reproductive success, plants have shifted flowering times to adapt to global warming. Although many studies focused on the molecular mechanisms of early flowering, little was supported by the repeated sampling at different time points through the changing climate. To fully dissect the temporal and spatial evolutionary genetics of flowering time, we investigated nucleotide variation in ten flowering time candidate genes and nine reference genes for the same ten wild-barley populations sampled 28 years apart (1980-2008). The overall genetic differentiation was significantly greater in the descendant populations (2008) compared with the ancestral populations (1980); however, local adaptation tests failed to detect any single-nucleotide polymorphism (SNP)/indel under spatial-diversifying selection at either time point. By contrast, the WFABC (Wright-Fisher ABC-based approach) that detected 54 SNPs/indels was under strong selection during the past 28 generations. Moreover, all these 54 alleles were segregated in the ancestral populations, but fixed in the descendent populations. Among the top ten SNPs/indels, seven were located in genes of FT1 (FLOWERING TIME LOCUS T 1), CO1 (CONSTANS-LIKE PROTEIN 1), and VRN-H2 (VERNALIZATION-H2), which have been documented to be associated with flowering time regulation in barley cultivars. This study might suggest that all ten populations have undergone parallel evolution over the past few decades in response to global warming, and even an overwhelming local adaptation and ecological differentiation.

3.
Mikrochim Acta ; 186(9): 626, 2019 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-31418087

RESUMO

The published version of this article, unfortunately, contains error. The author found out that the given name interchanged with the family name. The second author's family name should be "Yu" and the first name should be "Tao". Given in this article is the correct author name.

4.
Mikrochim Acta ; 186(9): 636, 2019 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-31432257

RESUMO

The preparation of an organic polymer monolithic column modified with an amino acid ionic liquid and graphene oxide (AAIL-GO) and its application to capillary electrochromatography (CEC) was described. The AAIL tetramethylammonium-L-arginine was bonded to a monolithic column that was previously modified with graphene oxide by using an hydrochloride/N-hydroxysuccinimide coupling reaction. The morphology of a poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolith was examined by scanning electron microscopy. The incorporation of AAIL and graphene oxide was detected by infrared spectroscopy and elemental analysis. The resulting monolithic column produced a strong and stable electroosmotic flow from the anode to the cathode in the pH range from 3 to 9. Compared with a column modified with AAIL or graphene oxide only, the AAIL-GO-modified column has a better separation ability for amino acids, ß-blockers, and nucleotides (the resolution of three amino acids: 2.231 and 2.036, ß-blockers: 2.779 and 2.470 and nucleotides: 8.345 and 3.321). Molecular modeling was applied to demonstrate the separation mechanism of small molecules which showed a good support for experimental results. Graphical abstract Schematic representation of capillary electrochromatography (CEC) systems with an amino acid ionic liquid-graphene oxide modified organic polymer monolithic column as stationary phases for separation of amino acids, ß-blockers, and nucleotides.

5.
Int J Biol Macromol ; 140: 119-128, 2019 Aug 14.
Artigo em Inglês | MEDLINE | ID: mdl-31419562

RESUMO

Cellulose/graphene oxide (CG) porous composites with 3D network structure were prepared via a solution mixing-regeneration and freeze-drying process. The CG aerogels were functionalized with 1,4-butane sultone under mild reaction conditions to achieve sulfated composite aerogels (SCGs), in which the sulfo groups were simultaneously introduced onto GO and cellulose components. The adsorption ability of SCG aerogel was greatly enhanced compared with CG aerogels. The fitting results of adsorption models suggested the monolayer adsorption and chemisorptive characteristics with the maximal uptake capacity as high as 421.9 mg/g for methylene blue (MB) and 163.4 mg/g for tetracycline (TC). The adsorption mechanism was also studied in detail. For the simulated wastewater containing MB and TC, the novel SCG adsorbent exhibited a removal efficiency of 99%. Furthermore, its adsorption capacity was not apparently deteriorated after seven cycles for MB and ten cycles for TC while the original structural integrity was almost maintained. Herein, this recyclable and reusable adsorbent exhibited the potential application on the removal of MB and TC from water.

6.
Anal Bioanal Chem ; 411(22): 5855-5866, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31286176

RESUMO

Recently, increasing attention has been given to the research on chiral ionic liquids (CILs) in chiral separation field; however, only a few literatures focus on the exploration of CILs as the sole chiral selector. In this study, an ionic liquid chiral selector based on antibiotic, namely tetramethylammonium clindamycin phosphate (TMA-CP), was originally synthesized and subsequently utilized for enantioseparation in capillary electrophoresis (CE). Remarkably improved separations of eight racemic analytes were achieved in TMA-CP system in contrast to the clindamycin phosphate (CP) system. The optimal separation conditions were determinated by systematic experiments on several crucial parameters including the type and proportion of organic modifier, CIL concentration, buffer pH, and applied voltage. Additionally, molecular modeling with AutoDock was applied to probe into the chiral recognition mechanism of the ionic liquid chiral selectors, which well corresponded with the experimental results. It is the first time that antibiotic-based ionic liquid was exploited as favorable sole chiral selector in CE, and this strategy has paved a new way for development of novel ionic liquids chiral selectors based on antibiotics. Graphical abstract.

7.
Mikrochim Acta ; 186(7): 462, 2019 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-31227901

RESUMO

This work shows that the metal organic framework (MOF) HKUST-1 of type Cu3(BTC)2 (also referred to as MOF-199; a face-centered-cubic MOF containing nanochannels) is a most viable coating for use in enantioseparation in capillary electrochromatography (CEC). A HKUST-1 modified capillary was prepared and characterized by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectra, elemental analysis and thermogravimetric analysis. CEC-based enantioseparation of the basic drugs propranolol (PRO), esmolol (ESM), metoprolol (MET), amlodipine (AML) and sotalol (SOT) was performed by using carboxymethyl-ß-cyclodextrin as the chiral selector. Compared with a fused-silica capillary, the resolutions are improved (ESM: 1.79; MET: 1.80; PRO: 4.35; SOT: 1.91; AML: 2.65). The concentration of chiral selector, buffer pH value, applied voltage and buffer concentration were optimized, and the reproducibilities of the migration times and Rs values were evaluated. Graphical abstract Schematic presentation of the preparation of a HKUST-1@capillary for enantioseparation of racemic drugs. Cu(NO3)2 and 1,3,5-benzenetricarboxylic acid (BTC) were utilized to prepare the HKUST-1@capillary. Then the capillary was applied to construct capillary electrochromatography system with carboxymethyl-ß-cyclodextrin (CM-ß-CD) for separation of basic racemic drugs.

8.
Arch Environ Occup Health ; : 1-14, 2019 Jun 18.
Artigo em Inglês | MEDLINE | ID: mdl-31210102

RESUMO

To investigate the contamination levels of respirable dust released in the work environment and the induced workers' health risk at a coal-fired power plant, we collected 405 dust samples from different dusty workstations by personal sampling during the coal-fired power generation process. Then, an inhalation risk assessment model from the USEPA was combined with the Monte Carlo simulation method to quantitatively evaluate the health risk caused by dust inhalation. Of 10 workstations researched, the dust concentration in the most workstations exceeded the prescribed occupational exposure limit. Workers engaged in ash removal suffered the highest health risk at 4.08 × 10-6 ± 2.85 × 10-6 (95% CI), closely followed by those involved in other job categories. The results can contribute to the formulation of targeted dust prevention measures and implementation of risk management for the coal-fired power sector.

9.
J Chromatogr A ; 1601: 340-349, 2019 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-31060783

RESUMO

In this study, three functionalized chiral ionic liquids (CILs) derived from l-valinol, l-prolinol and l-phenylalaninol, namely N,N,N-trimethyl-l-valinol-bis(trifluoromethanesulfon)imide ([TMLV]+[Tf2N]-, CIL1), N,N-dimethyl-l-prolinol-bis(trifluoromethanesulfon)imide ([DMLP]+[Tf2N]-, CIL2) and N,N,N-trimethyl-l-phenylalaninol-bis(trifluoromethanesulfon)imide ([TMLP]+[Tf2N]-, CIL3), were synthesized and subsequently utilized for enantiomeric separation in capillary electrophoresis (CE) with 2-hydroxypropyl-ß-cyclodextrin (HP-ß-CD) as chiral selector for the first time. Compared with traditional single HP-ß-CD separation system, the synergistic system exhibited substantially improved separations of six tested drugs. Using the CIL1/HP-ß-CD as a model system, the influence of crucial parameters including the type and proportion of organic modifier, CILs concentration, HP-ß-CD concentration and buffer pH was investigated in detail. Additionally, molecular modeling with AutoDock was applied to elucidate the enhanced enantioselectivity in the presence of CILs, which has certain guiding value in predicting the migration order of the enantiomers and studying the interactions important for the chiral recognition.


Assuntos
Amino Álcoois/química , Eletroforese Capilar/métodos , Líquidos Iônicos/síntese química , 2-Hidroxipropil-beta-Ciclodextrina/química , Concentração de Íons de Hidrogênio , Líquidos Iônicos/química , Estereoisomerismo
10.
Artigo em Inglês | MEDLINE | ID: mdl-31109043

RESUMO

China's coal mine production situation is grim and various types of accidents occur frequently, and gas explosion accidents are the highest incidence of coal mine accidents. The authors selected 200 gas explosion accidents of coal mine enterprises in recent years, and extracted a large number of workers' unsafe behaviors. Meanwhile, four working types related to gas explosion accidents were obtained, namely ventilation, gas prevention and fire extinguishing, blasting, and electrician. This article listed some influencing factors of unsafe behaviors and corrected the probability of unsafe behaviors. In addition, a probabilistic risk assessment model was established, and the Monte Carlo method was used to analyze the risks caused by unsafe behaviors of various working types. The results show that the risk of unsafe behaviors caused by the ventilation working type is the highest, followed by gas prevention and fire extinguishing, and finally blasting and electrician. This paper studies the influencing factors of miners' unsafe behaviors from the perspective of behavior, guarantees effectively the safety management of coal mine enterprises, and lays a foundation for studying unsafe behaviors related to coal mine gas explosions.

11.
Mikrochim Acta ; 186(4): 244, 2019 03 15.
Artigo em Inglês | MEDLINE | ID: mdl-30877441

RESUMO

Magnetic nanoparticles (MNPs) modified with ß-cyclodextrin and mono-6-deoxy-6-(1-methylimidazolium)-ß-cyclodextrin tosylate (an ionic liquid), which called MNP-ß-CD and MNP-ß-CD-IL, were coated into the capillary inner wall. Compared to an uncoated capillary, the new systems show good reproducibility and durability. The systems based on the use of MNP-ß-CD or MNP-ß-CD-IL as stationary phases were established for enantioseparation of Dns-modified amino acids. Improved resolutions were obtained for both CEC systems. Primary parameters such as running buffer pH value and applied voltage were systematically optimized in order to obtain optimal enantioseparations. Under the optimized conditions, the capillaries exhibited excellent chiral recognition ability for six Dns-amino acids (the DL-forms of alanine, leucine, lsoleucine, valine, methionine, glutamic acid) and provided a promising way for the preparation of chiral column. Graphical Abstract Schematic presentation of the open-tubular capillary electrochromatography systems with MNP-ß-CD and MNP-ß-CD-IL as stationary phases for enantioseparation of dansylated amino acids.

12.
Environ Geochem Health ; 41(5): 2179-2193, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30874937

RESUMO

With a growing awareness of environmental protection, the dust pollution caused by automobile foundry work has become a serious and urgent problem. This study aimed to explore contamination levels and health effects of automobile foundry dust. A total of 276 dust samples from six types of work in an automobile foundry factory were collected and analysed using the filter membrane method. Probabilistic risk assessment model was developed for evaluating the health risk of foundry dust on workers. The health risk and its influencing factors among workers were then assessed by applying the Monte Carlo method to identify the most significant parameters. Health damage assessment was conducted to translate health risk into disability-adjusted life year (DALY). The results revealed that the mean concentration of dust on six types of work ranged from 1.67 to 5.40 mg/m3. The highest health risks to be come from melting, cast shakeout and finishing, followed by pouring, sand preparation, moulding and core-making. The probability of the risk exceeding 10-6 was approximately 85%, 90%, 90%, 75%, 70% and 45%, respectively. The sensitivity analysis indicated that average time, exposure duration, inhalation rate and dust concentration (C) made great contribution to dust health risk. Workers exposed to cast shakeout and finishing had the largest DALY of 48.64a. These results can further help managers to fully understand the dust risks on various types of work in the automobile foundry factories and provide scientific basis for the management and decision-making related to health damage assessment.

13.
J Hazard Mater ; 373: 278-284, 2019 Jul 05.
Artigo em Inglês | MEDLINE | ID: mdl-30925387

RESUMO

The adsorption ability and photoactivity of a photocatalyst largely determine the mineralization efficiency of antibiotics. Herein, aiming to enhance the adsorption and mineralization of antibiotics, we constructed a hierarchical porous core-shell structure by filling amorphous TiO2 in the pores of Pt-doped mesoporous TiO2 crystals (MCs). The physical-chemical properties of the prepared samples were investigated by surface photovoltage spectroscopy, X-ray photoelectron spectroscope, etc. Adsorption and photocatalysis experiments were conducted with tetracycline hydrochloride as the model antibiotic. Pt nanoparticles doped at the interface of the rutile-amorphous homojunction remarkably enhanced the built-in electric field. The enhanced electric field increased the hole transfer to the catalyst surface, and the Pt doping treatment promoted the growth of amorphous TiO2 into the mesopores of the MCs. The optimization increased the surface area of the catalyst without increasing the thickness of the amorphous TiO2 shell, thereby reducing the charge migration distance from the core-shell interface to the catalyst surface. The adsorption amount and mineralization efficiency of tetracycline hydrochloride for the porous core-shell composite were 6.7 and 3.8 times of those for MCs, respectively.

14.
J Chromatogr A ; 1594: 199-207, 2019 Jun 07.
Artigo em Inglês | MEDLINE | ID: mdl-30837164

RESUMO

In this paper, a chiral selector, N-(4-H-1, 2, 4-triazolium)-lactobionamides (LA-ATM), was synthesized and applied for enantioseparation in capillary electrophoresis (CE) for the first time. Compared with lactobionic acid (LA) separation system, enhanced enantioseparation of five tested drugs was achieved in this modification system. In order to achieve good chiral separation, several parameters such as chiral selector concentration, buffer pH, applied voltage as well as the type and proportion of organic modifier were systematically investigated. Molecular modeling was applied to demonstrate the chiral recognition mechanism of the LA-ATM, which well supported the experimental results. Furthermore, a mathematical equation built up based on the molecular mechanics calculations was used in predicting resolution of tested drugs using LA or LA-ATM mediated CE, the predicted result was well correlated with the experimental result.


Assuntos
Amitrol (Herbicida)/síntese química , Dissacarídeos/síntese química , Eletroforese Capilar/métodos , Concentração de Íons de Hidrogênio , Modelos Moleculares , Estereoisomerismo
15.
Int Immunopharmacol ; 70: 135-146, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30802676

RESUMO

Acute lung injury (ALI) is a common clinical syndrome of excessive uncontrolled inflammatory response in lung tissues with high mortality rates and limited therapeutic approaches. MicroRNAs (miRNAs) are a class of small non-coding RNAs which attach at 3'UTR of mRNA for further regulation of diverse proteins. MiRNAs are a current focus in regulating the inflammatory processes. The extent of pro-inflammatory gene activated against Staphylococcus aureus (S. aureus) is still unclear. Myeloid differentiation primary response 88 (MyD88) is involved in gram positive bacteria-induced lung inflammation by Toll-like receptors (TLRs). Then MyD88 activates NF-κB through IRAKs which are in charge of inflammation. Target prediction analyses revealed MyD88, a result of projections from multiple bio-websites, to be a putative target of miR-128. Here we probe the expression of the MyD88 and miRNA in mode of inflammation. We found up-regulated expression of MyD88 and down-regulation of miR-128 after S. aureus infection in mouse lung tissues and RAW264.7 cells via qPCR and western blotting (WB) analysis. Moreover, MyD88-miR-128 interaction was validated by luciferase assays. Then, we proved that miR-128 expression caused a reduction in IκBα and p65 phosphorylation and resulted in significant reduction in secretion of inflammatory cytokines, being consistent with the deletion of MyD88 in macrophages. It revealed that miR-128 specifically blocked the further development of inflammation through MyD88 down-regulation. Finally, we demonstrated a novel role of miR-128 that it mediates negative regulation in S. aureus induced inflammation by targeting MyD88.


Assuntos
Lesão Pulmonar Aguda/imunologia , Inflamação/imunologia , Pulmão/imunologia , MicroRNAs/genética , Infecções Estafilocócicas/imunologia , Staphylococcus aureus/fisiologia , Lesão Pulmonar Aguda/genética , Animais , Regulação da Expressão Gênica , Inflamação/genética , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Fator 88 de Diferenciação Mieloide/metabolismo , NF-kappa B/genética , NF-kappa B/metabolismo , Células RAW 264.7 , Transdução de Sinais , Infecções Estafilocócicas/genética
16.
Sci Total Environ ; 664: 161-174, 2019 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-30739851

RESUMO

Water use efficiency (WUE) is an important variable used in hydrometeorology study to reveal the links between carbon-water cycles in sandy ecosystems which are highly sensitive to climate change and can readily reflect the effects of it. In light of the Paris Agreement, it is essential to identify the regional impacts of 0.5 °C of additional global warming to inform climate adaptation and mitigation strategies. Using the modified Carnegie-Ames-Stanford Approach (CASA) and Advection-Aridity (AA) models with global warming values of 1.5 °C and 2.0 °C above preindustrial levels from Inter-Sectoral Impact Model Intercomparison Project (ISIMIP2b) datasets, we conducted a new set of climate simulations to assess the effects of climate on WUE (the ratio of net primary productivity (NPP) to actual evapotranspiration (ETa)) in different sandy land types (mobile sandy land, MSL; semimobile/semifixed sandy land, SMSF; and fixed sandy land, FSL) during the period of baseline (1986-2005) and future (2006-2100). The spatiotemporal patterns of ETa, NPP, and WUE mostly showed increasing trends; the value of WUE decreased (6.40%) only in MSL with an additional 0.5 °C of warming. Meteorological and vegetation factors determined the variations in WUE. With warming, only the correlation between precipitation and WUE decreased in the three sandy land types, and the leaf area index (LAI) increased with an additional 0.5 °C of warming. The desertification degree comprehensively reflects the linkages among the standardized precipitation evapotranspiration index (SPEI), LAI and WUE. Simulation results indicated the sandy area extent could potential increase by 20 × 104 km2 per decade on average during 2016-2047 and that the increase could be gradual (2.60 × 104 km2 per decade) after 2050 (2050-2100). These results highlight the benefits of limiting the global mean temperature change to 1.5 °C above preindustrial levels and can help identify the risk of desertification with an additional 0.5 °C of warming.

17.
Artigo em Inglês | MEDLINE | ID: mdl-30717157

RESUMO

Miners' unsafe behavior is the main cause of roof accidents in coal mines, and behavior intervention plays a significant role in reducing the occurrence of miners' unsafe behavior. However, traditional behavior intervention methods lack pertinence. In order to improve the intervention effect and reduce the occurrence of coal mine roof accidents more effectively, this study proposed a targeted intervention method for unsafe behavior. The process of targeted intervention node locating was constructed, and based on the analysis of 331 coal mine roof accidents in China, three kinds of targeted intervention nodes were located. The effectiveness of targeted intervention nodes was evaluated by using structural equation model (SEM) through randomly distributing questionnaires to miners of Pingdingshan coal. The results show that, in preventing roof accidents of coal mines, the targeted intervention nodes have a significant positive impact on the intervention effect. The method can also be applied to the safety management of other industries by adjusting the node location and evaluation process.


Assuntos
Prevenção de Acidentes/métodos , Minas de Carvão , Comportamentos de Risco à Saúde/fisiologia , Mineradores/psicologia , Saúde do Trabalhador , Acidentes de Trabalho/prevenção & controle , Acidentes de Trabalho/estatística & dados numéricos , China , Humanos , Modelos Estatísticos , Gestão da Segurança/métodos
18.
Electrophoresis ; 40(15): 1921-1930, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-30663787

RESUMO

Recently, chiral ionic liquids (CILs) have received increasing interest in chiral separation by CE. Nevertheless, the lack of deep perception of the specific mechanism about CILs in CE for enantioseparation still perplexes a legion of researchers, despite the strenuous efforts. In this paper, a lactobionic acid based ionic liquid, tetramethylammonium-lactobionate (TMA-LA) was applied for the first time in CE to establish dual selector system with clindamycin phosphate (CP) for enantiomeric separation. Compared to single CP system or single TMA-LA system significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol) were observed in the dual selector systems. Several crucial parameters such as type and proportion of organic modifier, buffer composition and pH, and concentration of TMA-LA/CP were systematically investigated to achieve satisfied enantioseparation. Meanwhile, molecular modeling was applied to demonstrate the chiral recognition mechanism of the TMA-LA/CP dual-selector separation system using the molecular docking software Autodock, which well supported the experimental results. The existence of TMA-LA/CP complex may give rise to a higher discriminatory ability against the enantiomers, indicating the reason of improved separation in TMA-LA/CP system. All the influence factors evaluated by means of Statistical Product and Service Solutions (SPSS) to research the influences on the chiral separation system.

19.
Inflamm Res ; 68(3): 231-240, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30673803

RESUMO

OBJECTIVE: In both humans and animals, endometritis is severe inflammation of the uterus, and it causes great economic losses in dairy cow production. MicroRNAs have been reported to play an important role in various inflammatory diseases. However, the regulatory mechanisms of miR-19a in endometritis remain unclear. Thus, the aims of this study are to investigate the role of miR-19a in a mouse model of lipopolysaccharide (LPS)-induced endometritis and elucidate the possible mechanisms in bovine endometrial epithelial cells (bEECs). METHODS AND RESULTS: Histological analysis showed that LPS induced severe pathological changes, suggesting that the endometritis mouse model was well established. The qPCR assay indicated that miR-19a expression in the uterine tissues of mice with endometritis and in bEECs with LPS stimulation was significantly reduced. The overexpression of miR-19a significantly decreased the expression of inflammatory cytokines (TNF-α, IL-6 and IL-1ß) and the phosphorylation of NF-κB p65 and IκBα. Similar results were also obtained following the knockdown of TBK1. Furthermore, a dual luciferase reporter assay further validated that miR-19a inhibited TBK1 expression by binding directly to the 3'-UTR of TBK1. CONCLUSION: We demonstrated that miR-19a has anti-inflammatory effects and mediates the negative regulation of the NF-κB Pathway in LPS-induced endometritis by targeting TBK1.


Assuntos
Endometrite/imunologia , MicroRNAs/fisiologia , NF-kappa B/imunologia , Proteínas Serina-Treonina Quinases/imunologia , Animais , Bovinos , Linhagem Celular , Citocinas/imunologia , Endometrite/induzido quimicamente , Endometrite/patologia , Feminino , Inativação Gênica , Humanos , Lipopolissacarídeos , Camundongos Endogâmicos BALB C , MicroRNAs/genética , Proteínas Serina-Treonina Quinases/genética , Transdução de Sinais , Útero/imunologia , Útero/patologia
20.
Front Genet ; 9: 506, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30487810

RESUMO

As one of the hotspot regions for sympatric speciation studies, Evolution Canyon (EC) became an ideal place for its high level of microclimatic divergence interslopes. In this study, to highlight the genetic mechanisms of sympatric speciation, phenotypic variation on flowering time and transcriptomic divergence were investigated between two ecotypes of Ricotia lunaria, which inhabit the opposite temperate and tropical slopes of EC I (Lower Nahal Oren, Mount Carmel, Israel) separated by 100 m at the bottom of the slopes. Growth chamber results showed that flowering time of the ecotype from south-facing slope population # 3 (SFS 3) was significantly 3 months ahead of the north-facing slope population # 5 (NFS 5). At the same floral development stage, transcriptome analysis showed that 1,064 unigenes were differentially expressed between the two ecotypes, which enriched in the four main pathways involved in abiotic and/or biotic stresses responses, including flavonoid biosynthesis, α-linolenic acid metabolism, plant-pathogen interaction and linoleic acid metabolism. Furthermore, based on Ka/Ks analysis, nine genes were suggested to be involved in the ecological divergence between the two ecotypes, whose homologs functioned in RNA editing, ABA signaling, photoprotective response, chloroplasts protein-conducting channel, and carbohydrate metabolism in Arabidopsis thaliana. Among them, four genes, namely, SPDS1, FCLY, Tic21 and BGLU25, also showed adaptive divergence between R. lunaria and A. thaliana, suggesting that these genes could play an important role in plant speciation, at least in Brassicaceae. Based on results of both the phenotype of flowering time and comparative transcriptome, we hypothesize that, after long-time local adaptations to their interslope microclimatic environments, the molecular functions of these nine genes could have been diverged between the two ecotypes. They might differentially regulate the expression of the downstream genes and pathways that are involved in the interslope abiotic stresses, which could further diverge the flowering time between the two ecotypes, and finally induce the reproductive isolation establishment by natural selection overruling interslope gene flow, promoting sympatric speciation.

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