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1.
Artigo em Inglês | MEDLINE | ID: mdl-34636553

RESUMO

The development of stable, low-cost, and highly efficient electrocatalysts for the N2 reduction reaction (NRR) process is challenging but crucial for ammonia production. Herein, we demonstrate the synthesis of pyrite nanoparticles wrapped by graphene oxide (FeS2@GO) acting as a highly efficient NRR catalyst in a wide pH range. The FeS2 nanoparticles are uniformly dispersed across the GO nanosheet, thus leading to the fine exposure of active sites, the promotion of charge transfer, and the increment of a contact surface area, which are all beneficial for a desired catalyst. In the meantime, the low-coordinated Fe atoms are activated as highly active sites, which is in favor of the enhanced electrochemical performance for the NRR. Furthermore, density functional theory (DFT) calculations illustrated that the high activity of N2 reduction over the FeS2@GO catalyst arises from the well-exposed Fe active sites and the increment of charge density at the valence band edge. Benefiting from the well-optimized interface, the barrier of the addition of the first hydrogen atom to N2 forming *NNH species as the potential-determining step is as low as 0.93 eV in N2 electroreduction. The electrochemical test results reveal that, as expected, FeS2@GO exhibits high Faradaic efficiencies (4.7% in 0.1 M HCl solution and 6.8% in 0.1 M Na2SO4 solution) and advanced NH3 yields (78.6 and 27.9 µg h-1 mgcat.-1 in 0.1 M HCl and 0.1 M Na2SO4 solutions, respectively) in both acid and neutral conditions. This work offers a new avenue for exploring novel electrocatalysts, which has great promise to accelerate the practical application of the NRR.

3.
Zhongguo Zhong Yao Za Zhi ; 46(13): 3257-3269, 2021 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-34396745

RESUMO

Cardiovascular diseases seriously endanger human health and life. The accompanying myocardial injury has been a focus of attention in society. Chinese medicine,serving as a natural and precious reservoir for the research and development of new drugs,is advantageous in resisting myocardial injury due to its multi-component,multi-pathway,and multi-target characteristics. In recent years,with the extensive application of culture method for isolated cardiomyocytes,a cost-effective,controllable in vitro model of cardiomyocyte injury with uniform samples is becoming a key tool for mechanism research on cardiomyocyte injury and drug development.A good in vitro model can reduce experimental and manpower cost,and also accurately stimulate clinical changes to reveal the mechanism. Therefore,the selection and establishment of in vitro model are crucial for the in-depth research. This study summarized the modeling principles,evaluation indicators,and application of more than ten models reflecting different clinical conditions,such as injuries induced by hypoxia-reoxygenation,hypertrophy,oxidative stress,inflammation,internal environmental disturbance,and toxicity. Furthermore,we analyzed advantages and technical difficulties,aiming to provide a reference for in-depth research on myocardial injury mechanism and drug development.


Assuntos
Apoptose , Miócitos Cardíacos , Hipóxia Celular , Humanos , Miocárdio , Estresse Oxidativo
4.
Neurochem Res ; 46(9): 2276-2284, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34081245

RESUMO

Diabetic neuropathy is one of the common complications of type 2 diabetes mellitus (T2DM) with severe outcomes. The mechanisms of physiopathology of diabetic neuropathy are not well elucidated. Inflammation and inflammatory macrophages are recognized to be crucial in diabetic neuropathy. Toll-like receptor 2 (TLR2) is an important factor in innate immune response which could promote the polarization of inflammatory macrophages. In present study, we evaluated the effects of a TLR2 antagonist CU-CPT22 on diabetic neuropathy. We induced T2DM in mice by feeding with high fat diet (HFD). We measured the body weight, blood glucose level, paw withdrawal threshold, inflammatory cytokine production, and macrophages infiltration in T2DM mice. We evaluated the effects of CU-CPT22 on pro-inflammatory cytokines production, macrophage marker expression in lipopolysaccharides (LPS)-treated BMDMs. We administrated CU-CPT22 in T2DM mice and measured the pro-inflammatory cytokines levels, expression of macrophages markers in sciatic nerve (SCN), and paw withdrawal threshold. T2DM mice had significantly increased body weight and blood glucose, and had significantly decreased paw withdrawal threshold. Obvious increased pro-inflammatory cytokine level and infiltration of M1 phenotype macrophages was observed in SCN from T2DM mice. CU-CPT22 prevented pro-inflammatory cytokine production in LPS-treated BMDMs and re-polarized them to M2 phenotype. CU-CPT22 suppressed the inflammation and induced M2 macrophages in SCN from T2DM mice, and ameliorated the paw withdrawal threshold in T2DM mice. CU-CPT22 ameliorates neuropathic pain in T2DM by promoting M2 phenotype macrophages.

5.
Artigo em Inglês | MEDLINE | ID: mdl-34167955

RESUMO

INTRODUCTION: Saliva collection is a non-invasive test and is convenient. 1,5-anhydroglucitol (1,5-AG) is a new indicator reflecting short-term blood glucose levels. This study aimed to explore the relationship between saliva 1,5-AG and insulin secretion function and insulin sensitivity. RESEARCH DESIGN AND METHODS: Adult patients with type 2 diabetes who were hospitalized were enrolled. Based on blood glucose and C-peptide, homeostasis model assessment 2 for ß cell secretion function, C-peptidogenic index (CGI), △2-hour C-peptide (2hCP)/△2-hour postprandial glucose (2hPG), ratio of 0-30 min area under the curve for C-peptide and area under the curve for glucose (AUCCP30/AUCPG30), and AUC2hCP/AUC2hPG were calculated to evaluate insulin secretion function, while indicators such as homeostasis model assessment 2 for insulin resistance were used to assess insulin sensitivity. RESULTS: We included 284 subjects (178 men and 106 women) with type 2 diabetes aged 20-70 years. The saliva 1,5-AG level was 0.133 (0.089-0.204) µg/mL. Spearman's correlation analysis revealed a significantly negative correlation between saliva 1,5-AG and 0, 30, and 120 min blood glucose, glycated hemoglobin A1c, and glycated albumin (all p<0.05), and a significantly positive association between saliva 1,5-AG and CGI (r=0.171, p=0.004) and AUC CP30 /AUC PG30 (r=0.174, p=0.003). The above correlations still existed after adjusting for age, sex, body mass index, and diabetes duration. In multiple linear regression, saliva 1,5-AG was an independent factor of CGI (standardized ß=0.135, p=0.015) and AUC CP30 /AUC PG30 (standardized ß=0.110, p=0.020). CONCLUSIONS: Saliva 1,5-AG was related to CGI and AUCCP30/AUCPG30 in patients with type 2 diabetes. TRIAL REGISTRATION NUMBER: ChiCTR-SOC-17011356.


Assuntos
Diabetes Mellitus Tipo 2 , Adulto , China , Desoxiglucose , Diabetes Mellitus Tipo 2/diagnóstico , Feminino , Teste de Tolerância a Glucose , Humanos , Insulina/metabolismo , Secreção de Insulina , Masculino , Saliva/metabolismo
6.
Front Med ; 15(3): 460-471, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-34185279

RESUMO

Lifestyle interventions, including dietary adjustments and exercise, are important for obesity management. This study enrolled adults with overweight or obesity to explore whether either low-carbohydrate diet (LCD) or exercise is more effective in metabolism improvement. Forty-five eligible subjects were randomly divided into an LCD group (n = 22) and an exercise group (EX, n = 23). The subjects either adopted LCD (carbohydrate intake < 50 g/day) or performed moderate-to-vigorous exercise (⩾ 30 min/day) for 3 weeks. After the interventions, LCD led to a larger weight loss than EX ( - 3.56 ± 0.37 kg vs. - 1.24 ± 0.39 kg, P < 0.001), as well as a larger reduction in fat mass ( - 2.10 ± 0.18 kg vs. - 1.25 ± 0.24 kg, P = 0.007) and waist circumference ( - 5.25 ± 0.52 cm vs. - 3.45 ± 0.38 cm, P = 0.008). Both interventions reduced visceral and subcutaneous fat and improved liver steatosis and insulin resistance. Triglycerides decreased in both two groups, whereas low-density lipoprotein cholesterol increased in the LCD group but decreased in the EX group. Various glycemic parameters, including serum glycated albumin, mean sensor glucose, coefficient of variability (CV), and largest amplitude of glycemic excursions, substantially declined in the LCD group. Only CV slightly decreased after exercise. This pilot study suggested that the effects of LCD and exercise are similar in alleviating liver steatosis and insulin resistance. Compared with exercise, LCD might be more efficient for weight loss and glucose homeostasis in people with obesity.


Assuntos
Dieta com Restrição de Carboidratos , Perda de Peso , Adulto , Glicemia , Homeostase , Humanos , Projetos Piloto
7.
Zhongguo Zhong Yao Za Zhi ; 46(9): 2254-2259, 2021 May.
Artigo em Chinês | MEDLINE | ID: mdl-34047128

RESUMO

Rhus chinensis is an important resource plant. The aqueous extract of R. chinensis roots or stems was to produce Shuguantong Syrup, which is mainly used for the treatment of coronary heart disease and angina pectoris with definite curative effect. On this basis, the crude phenolic part of R. chinensis prepared by macroporous resin was evaluated for the cardio protective effect against myocardial ischemia in mice. The results showed that the phenolic part group with oral administration at the dosages of 190.8-381.6 mg·kg~(-1), compared with the model group, reduced the values of left ventricular end systolic diameter(LVEDs) and the left ventricular end diastolic diameter(LVEDd), and increased the cardiac ejection fraction(EF) and left ventricular fractional shortening(FS) rate, which could effectively improve cardiac function and exert its anti-myocardial ischemia effect, and reduce the rising levels of creatine kinase isoenzyme(CK-MB) and lactate dehydrogenase(LDH) in serum. HE staining showed that the phenolic part group reduced the infiltration of myocardial inflammatory cells and alleviated the degree of myocardial fibrosis and collagen deposition. TUNEL staining showed that the blue-green fluorescence of the phenolic part group decreased successively, and the degree of myocardial cell apoptosis was reduced. Immunohistochemical staining suggested that it could reduce the number of positive cells for p53 protein expression and significantly improve myocardial cell damage. All above data suggested that the phenolic part group had an anti-mycardial ischemis effect. Related mechanism studies revealed that the crude phenolic part could regulate the expressions of the p53 gene(p53), Bcl-2-associated X protein(Bax), B lymphoma-2 gene(Bcl-2), and caspase-3 protein(caspase-3) in myocardial tissue, suggesting that it could reduce cardiac remodeling and myocardial ischemic damage, and improve cardiac function by inhibiting myocardial apoptosis.This research laid a foundation for the elucidation of the pharmacological ingredients R. chinensis.


Assuntos
Isquemia Miocárdica , Extratos Vegetais/farmacologia , Rhus , Animais , Apoptose , Camundongos , Isquemia Miocárdica/tratamento farmacológico , Miocárdio , Miócitos Cardíacos , Proteína X Associada a bcl-2
8.
Langmuir ; 2021 May 12.
Artigo em Inglês | MEDLINE | ID: mdl-33979523

RESUMO

Removal of oil from water is strongly desired due to environmental pollution, and related studies are mainly limited to the material itself. Here, we propose an oil-water separation device, called a floating well, which includes a container for oil collection and a modified mesh screen for oil separation. The mesh screen is superhydrophilic to oil and superhydrophobic to water. The oil removal experiment was performed in a basin. Under the calm surface condition, the oil collection efficiency is shown to be 42-69%. The inadequate oil collection is explained by the breakdown of a thin oil film above water to form an oil-free area, preventing direct contact between the separator and the oil. Sustained by a wave generator, we are surprised to find that with a low-frequency surface wave, the oil collection efficiency is increased to 98%, and the collection speed reaches 2.5 times that under the calm surface condition. The almost complete collection is due to the sustained contact between the separator and the oil, under which the surface wave continuously drives the oil film toward the separator from elsewhere, thus the oil-free area cannot be formed. Our work presents a new clue for large-scale in situ applications, in which the nature wave energy of river/sea can be the driving force for continuous oil separation and collection.

9.
Acta Diabetol ; 58(9): 1217-1224, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-33871690

RESUMO

AIMS: Atherosclerotic cardiovascular disease remains the leading cause of death among patients with diabetes. Early identification of subclinical atherosclerosis is essential for the management of diabetic patients. This study aimed to characterize serum metabolic signatures associated with carotid intima-media thickness (C-IMT), a proxy of subclinical atherosclerosis, in patients with type 2 diabetes mellitus (T2DM). METHODS: After 1:1 matching by sex, age, body mass index, glycated haemoglobin A1c, and other clinical parameters, a total of 462 T2DM patients were enrolled, consisting of 231 patients with C-IMT of ≥ 1 mm (abnormal C-IMT) and 231 patients with C-IMT of < 1 mm (normal C-IMT). C-IMT was assessed using ultrasonography. The serum metabolic profiling of fasting blood samples was performed using liquid chromatography-tandem triple quadrupole mass spectrometer coupled with the multivariate and univariate statistical analysis. RESULTS: Patients with abnormal C-IMT had significantly higher deoxycholic acid (DCA) and taurodeoxycholic acid (TDCA) levels, and lower levels of taurocholic acid (TCA) than those with normal C-IMT. Conditional logistic regression analysis revealed that per 1-standard deviation increase of DCA, TDCA and TCA were significantly associated with 64.7% (95% CI: 1.234-2.196) and 38.5% (95% CI: 1.124-1.706) higher, and 26.8% (95% CI: 0.597-0.897) lower risk of abnormal C-IMT, after adjustment of confounders. The addition of DCA, TCA, or DCA × TDCA/TCA ratio significantly improved the discrimination of abnormal C-IMT over traditional risk factors. CONCLUSIONS: Serum bile acids may be potential biomarkers for subclinical atherosclerosis in T2DM patients, which needs further confirmation.


Assuntos
Aterosclerose , Diabetes Mellitus Tipo 2 , Aterosclerose/complicações , Aterosclerose/diagnóstico , Espessura Intima-Media Carotídea , Diabetes Mellitus Tipo 2/complicações , Jejum , Humanos , Fatores de Risco
10.
Cell Rep ; 35(1): 108948, 2021 04 06.
Artigo em Inglês | MEDLINE | ID: mdl-33826900

RESUMO

Fascin protein is the main actin-bundling protein in filopodia and invadopodia, which are critical for tumor cell migration, invasion, and metastasis. Small-molecule fascin inhibitors block tumor invasion and metastasis and increase the overall survival of tumor-bearing mice. Here, we report a finding that fascin blockade additionally reinvigorates anti-tumor immune response in syngeneic mouse models of various cancers. Fascin protein levels are increased in conventional dendritic cells (cDCs) in the tumor microenvironment. Mechanistically, fascin inhibitor NP-G2-044 increases the number of intratumoral-activated cDCs and enhances the antigen uptake by cDCs. Furthermore, together with PD-1 blocking antibody, NP-G2-044 markedly increases the number of activated CD8+ T cells in the otherwise anti-PD-1 refractory tumors. Reduction of fascin levels in cDCs, but not fascin gene knockout in tumor cells, mimics the anti-tumor immune effect of NP-G2-044. These data demonstrate that fascin inhibitor NP-G2-044 simultaneously limits tumor metastasis and reinvigorates anti-tumor immune responses.

11.
Langmuir ; 2021 Apr 06.
Artigo em Inglês | MEDLINE | ID: mdl-33822627

RESUMO

The contact angle of a nanodroplet on a surface may deviate from that of a macrodroplet on a surface. Even though there are many studies regarding line tension, it is not well understood. In this paper, molecular dynamics simulation is performed for nanodroplets on a concave solid wall. The Lennard-Jones (L-J) potential is directly used or modified to simulate the force interaction between argon atoms and between solid-liquid particles. The initial droplet radius is 4, 5, and 6 nm, respectively. The k coefficient is defined as the ratio of the initial droplet radius with respect to the curvature radius of concave walls, which is in the range of 0-0.9, in which k = 0 refers to a flat surface. We found that indeed the contact angle θ of a nanodroplet on a concave wall deviates from that of a macrodroplet on a flat surface. Contact angles display a two region distribution, in which θ increases with increasing k for k < 0.5 and decreases with increasing k for k > 0.5. The k coefficient influences the droplet morphology. With k in the range of 0-0.9, the vapor-liquid interface is switched from a convex shape to a flat shape and finally reaches a concave shape. The line tension generally behaves in an increasing trend with the increase of k but becomes constant when k is beyond 0.7. The liquid densities, radial distribution functions, and coordination numbers show that the liquid particles are more closely packed with each other with the increase of k. The line tension achieves a positive sign and on the magnitude of 10-11 N, which has a linear increase with respect to the peak density of the first liquid layer.

12.
Cancer Immunol Immunother ; 70(10): 2925-2935, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-33710368

RESUMO

PURPOSE: Identification of reliable postoperative indicators for accurately evaluating prognosis of clear cell renal cell carcinoma (ccRCC) patients remains an important clinical issue. This study determined the prognostic value of UBR5 expression in ccRCC patients by combining with CD163+ tumor-associated macrophages (TAMs) and the established clinical parameters. METHODS: The expression of UBR5 was analyzed in ccRCC patients from TCGA databases. A total of 310 ccRCC patients were randomly divided into the training and validation cohorts at a 3:2 or 1:1 ratio, and immunohistochemistry (IHC) and statistical analyses were performed to examine the prognostic value of UBR5 and CD163+ TAMs. RESULTS: UBR5 expression was commonly downregulated in human ccRCC specimens, which was associated with TNM stage, SSIGN, WHO/ISUP Grading and poor prognosis of ccRCC patients. In addition, UBR5 expression was negatively correlated with CD163 expression (a TAM marker) in ccRCC tissues, and combining expressions of UBR5 and CD163 better predicted worse overall survival and progression-free survival of ccRCC patients. Even after multivariable adjustment, UBR5, CD163, TNM stage and SSIGN appeared to be independent risk factors. By time-dependent c-index analysis, the integration of intratumoral UBR5 and CD163 achieved higher c-index value than UBR5, CD163, TNM stage or SSIGN alone in predicting ccRCC patients' prognosis. Moreover, the incorporation of both UBR5 and CD163 into the clinical indicators TNM stage or SSIGN exhibited highest c-index value. CONCLUSIONS: Integrating intratumoral UBR5 and CD163+ TAMs with the current clinical parameters achieves better accuracy in predicting ccRCC patients' postoperative prognosis.


Assuntos
Antígenos CD/metabolismo , Antígenos de Diferenciação Mielomonocítica/metabolismo , Carcinoma de Células Renais/genética , Neoplasias Renais/genética , Macrófagos/metabolismo , Receptores de Superfície Celular/metabolismo , Ubiquitina-Proteína Ligases/metabolismo , Carcinoma de Células Renais/patologia , Feminino , Humanos , Neoplasias Renais/patologia , Masculino , Prognóstico , Estudos Retrospectivos , Fatores de Risco
13.
Nano Lett ; 2021 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-33646790

RESUMO

Materials with interfaces often exhibit extraordinary phenomena exemplified by rich physics, such as high-temperature superconductivity and enhanced electronic correlations. However, demonstrations of confined interfaces to date have involved intensive effort and fortuity, and no simple path is consistently available. Here, we report the achievement of interfacial superconductivity in the nonsuperconducting parent compounds AEFe2As2, where AE = Ca, Sr, or Ba, by simple subsequent annealing of the as-grown samples in an atmosphere of As, P, or Sb. Our results indicate that the superconductivity originates from electron transfer at the interface of the hybrid van der Waals heterostructures, consistent with the two-dimensional superconducting transition observed. The observations suggest a common origin of interfaces for the nonbulk superconductivity previously reported in the AEFe2As2 compound family and provide insight for the further exploration of interfacial superconductivity.

14.
Nat Commun ; 12(1): 1487, 2021 03 05.
Artigo em Inglês | MEDLINE | ID: mdl-33674561

RESUMO

Hyocholic acid (HCA) is a major bile acid (BA) species in the BA pool of pigs, a species known for its exceptional resistance to spontaneous development of diabetic phenotypes. HCA and its derivatives are also present in human blood and urine. We investigate whether human HCA profiles can predict the development of metabolic disorders. We find in the first cohort (n = 1107) that both obesity and diabetes are associated with lower serum concentrations of HCA species. A separate cohort study (n = 91) validates this finding and further reveals that individuals with pre-diabetes are associated with lower levels of HCA species in feces. Serum HCA levels increase in the patients after gastric bypass surgery (n = 38) and can predict the remission of diabetes two years after surgery. The results are replicated in two independent, prospective cohorts (n = 132 and n = 207), where serum HCA species are found to be strong predictors for metabolic disorders in 5 and 10 years, respectively. These findings underscore the association of HCA species with diabetes, and demonstrate the feasibility of using HCA profiles to assess the future risk of developing metabolic abnormalities.


Assuntos
Biomarcadores/sangue , Ácidos Cólicos/sangue , Ácidos Cólicos/urina , Doenças Metabólicas/diagnóstico , Adulto , Estudos de Coortes , Estudos Transversais , Diabetes Mellitus Tipo 2/metabolismo , Fezes/química , Feminino , Humanos , Estudos Longitudinais , Masculino , Pessoa de Meia-Idade , Obesidade/metabolismo , Sobrepeso/metabolismo , Estado Pré-Diabético/diagnóstico , Estudos Prospectivos
15.
Pediatr Int ; 63(7): 757-763, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33600060

RESUMO

BACKGROUND: We aimed to assess the efficacy of different initial intravenous immunoglobulin (IVIG) regimens in Kawasaki disease (KD) patients to find more cost-effective therapy options. METHODS: A multicenter, open-label, blind-endpoint randomized controlled trial was conducted from January 2014 to December 2015. Patients with KD, within 10 days of illness, were randomly assigned to receive different IVIG regimens (Group A, 2 g/kg once; Group B, 1 g/kg for 2 consecutive days; Group C, 1 g/kg once) and aspirin 30mg/kg/d. Primary outcomes included hours to defervescence and development of coronary artery lesions during the study period. Major secondary outcomes included total fever days, total dose of IVIG, changes of laboratory data, length of stay, and hospitalization expenses. (ClinicalTrials.gov: NCT02439996). RESULTS: A total of 404 patients underwent randomization. No difference was found in the outcomes of defervescence among three groups at 6, 12, 24, and 36 hours after completion of initial IVIG infusion. There were no differences in the incidence of coronary artery lesions during the study period (at week 2, month 1, month 3, and month 6 of illness), changes of laboratory data, total fever days, and length of stay. Group C patients had the lowest total dose of IVIG (mean: 1.2 vs 2.2 vs 2.1 g/kg; P < 0.001) and hospitalization expenses (mean: 8443.8 vs 10798.4 vs 11011.4 Chinese Yuan; P < 0.001) than other two groups. CONCLUSIONS: A single dose of 1g/kg IVIG is a low-cost treatment with the same efficacy as 2 g/kg IVIG and can be an option for the initial therapy of KD patients.


Assuntos
Imunoglobulinas Intravenosas , Síndrome de Linfonodos Mucocutâneos , Aspirina , Febre , Humanos , Imunoglobulinas Intravenosas/uso terapêutico , Lactente , Síndrome de Linfonodos Mucocutâneos/tratamento farmacológico
16.
Appl Microbiol Biotechnol ; 105(5): 2033-2042, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33582833

RESUMO

Liver cancer, one of the most common types of cancer in the world, is the second leading cause of death for cancer patients. For liver cancer, there is an urgent need for an effective treatment with no or less toxic side effects. Lactonic sophorolipids (LSL), as a potential anticancer drug, has attracted wide attention of pharmaceutical researchers with its good biological activities. The effects of LSL and cell death inhibitors were measured by MTT test on HepG2 cells. Meanwhile, the morphology of the cells was observed under a microscope. The apoptosis rate was detected by flow cytometry, and the expression levels of enzyme activity of Caspase-3 and Caspase-9 were measured by detection kits. Meanwhile, mRNA levels of Apaf-1, Caspase-3, Bax, and Bcl-2 were measured by quantitative real-time RT-PCR; protein levels of Caspase-3, Cleaved Caspase-3, Bax, and Bcl-2 were measured by western blot. LSL can inhibit the proliferation of cells, and it is possible to induce apoptosis in cells. The HepG2 cells with LSL co-culture exhibited typical apoptotic morphology, and the expression levels of enzyme activity of Caspase-3 and Caspase-9 increased (P< 0.05). We also found that LSL increases cell apoptosis rate and regulates the expression of genes and proteins associated with apoptosis through the Caspase-3 pathway. These results indicate that LSL may be one of the potential drug candidates to inhibit the proliferation and induce apoptosis in HepG2 cells.Key points• LSL, which is of good biological activities such as anti-bacterium, virus elimination, and inflammatory response elimination, has been firstly used to intervene in vitro to investigate its effect on HepG2 cell proliferation.• LSL can inhibit the proliferation of cells, and it is possible to induce apoptosis in HepG2 cells through the Caspase-3 pathway.• The mechanism of LSL action on HepG2 cell proliferation was firstly also discussed, which provides a certain experimental reference for the clinical treatment of liver cancer.


Assuntos
Carcinoma Hepatocelular , Neoplasias Hepáticas , Apoptose , Caspase 3/genética , Proliferação de Células , Glicolipídeos , Células Hep G2 , Humanos
17.
Ann Thorac Surg ; 112(3): e177-e180, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-33515543

RESUMO

Cardiac fibroma is a rare disease, and surgical resection generally remains the treatment of choice for these tumors in children. However, open surgery for some patients has to be rejected due to high risk. Here, we report a case of somewhat successful radiofrequency ablation of a huge left ventricular fibroma in a 3-year-old asymptomatic child. At the 3-month follow-up, the child was clinically well. Our case highlights that radiofrequency ablation may be used as an alternative treatment for the cardiac tumors, which are unsuitable for cardiac operation.


Assuntos
Fibroma/cirurgia , Neoplasias Cardíacas/cirurgia , Ventrículos do Coração , Ablação por Radiofrequência , Pré-Escolar , Humanos
18.
Front Med ; 15(1): 91-100, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32820380

RESUMO

Congenital heart disease (CHD) is the most common birth defect worldwide. Long non-coding RNAs (lncRNAs) have been implicated in many diseases. However, their involvement in CHD is not well understood. This study aimed to investigate the role of dysregulated lncRNAs in CHD. We used Gene Expression Omnibus data mining, bioinformatics analysis, and analysis of clinical tissue samples and observed that the novel lncRNA SAP30-2:1 with unknown function was significantly downregulated in damaged cardiac tissues from patients with CHD. Knockdown of lncRNA SAP30-2:1 inhibited the proliferation of human embryonic kidney and AC16 cells and decreased the expression of heart and neural crest derivatives expressed 2 (HAND2). Moreover, lncRNA SAP30-2:1 was associated with HAND2 by RNA immunoprecipitation. Overall, these results suggest that lncRNA SAP30-2:1 may be involved in heart development through affecting cell proliferation via targeting HAND2 and may thus represent a novel therapeutic target for CHD.


Assuntos
Cardiopatias Congênitas , RNA Longo não Codificante , Fatores de Transcrição Hélice-Alça-Hélice Básicos , Proliferação de Células , Cardiopatias Congênitas/genética , Histona Desacetilases , Humanos , RNA Longo não Codificante/genética , Fatores de Transcrição
19.
Gen Thorac Cardiovasc Surg ; 69(2): 356-359, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32749598

RESUMO

Primary cardiac lymphoma (PCL) is one of the rarest tumors and the most common type is large B-cell lymphoma. The right atrium and right ventricle are the most often involved, which should be diagnosed and treated in time. In this case, a female patient underwent an emergency surgical treatment for a large pericardial effusion and a huge mass in the right atrium. The huge mass was resected successfully. The pathological evaluation suggested that PCL and staging systemic chemotherapy were initiated according to the result, which significantly improve the prognosis of and quality of life.


Assuntos
Tamponamento Cardíaco , Neoplasias Cardíacas , Linfoma Difuso de Grandes Células B , Derrame Pericárdico , Tamponamento Cardíaco/etiologia , Tamponamento Cardíaco/cirurgia , Feminino , Neoplasias Cardíacas/complicações , Neoplasias Cardíacas/diagnóstico por imagem , Neoplasias Cardíacas/cirurgia , Humanos , Linfoma Difuso de Grandes Células B/complicações , Linfoma Difuso de Grandes Células B/diagnóstico , Linfoma Difuso de Grandes Células B/tratamento farmacológico , Derrame Pericárdico/etiologia , Derrame Pericárdico/cirurgia , Qualidade de Vida
20.
J Mol Neurosci ; 71(5): 972-980, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33009636

RESUMO

Accumulating evidence has elucidated that human mesenchymal stem cells (hMSCs) exert profound analgesic effects on numerous animal models of neuropathic pain, including drug-induced peripheral nerves, diabetes-induced neuropathy, and chronic constriction injury. We aimed to address whether forcing expression of sirtuin 1 (SIRT1) can enhance the efficacy of hMSCs on alleviation of pain sensation. A rat model of chronic constriction injury (CCI) mimicking peripheral nerve injury was incorporated in the study. Paw withdrawal threshold (PWT) and paw withdrawal latency (PWL) testing were used to measure pain-related behavior. Our results demonstrated that SIRT1 was decreased post-CCI surgery. Compared to hMSCs-control implantation, the hMSCs-SIRT1 (hMSCs overexpressing SIRT1) implantation exhibited superior effects on reducing pro-inflammatory cytokine levels in serum and spinal dorsal horn, while ameliorating neuropathic pain in CCI rat. Engineering hMSCs such as overexpressing SIRT1 may serve as a promising strategy for the treatment of patients with neuropathic pain.

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