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1.
Artigo em Inglês | MEDLINE | ID: mdl-34596622

RESUMO

ABSTRACT: Endothelial dysfunction participates in the pathogenesis of various cardiovascular disorders, and dysregulated angiogenesis involves the vascular endothelial growth factor (VEGF)-matrix metalloproteinases (MMP) system. NAMPT (Nicotinamide phosphoribosyltransferase) is known to enhance endothelial function and angiogenesis. The study found that NAMPT overexpression protected human coronary artery endothelial cells from H2O2-induced injury through promoting cell viability, inhibiting cell apoptosis, enhancing cell motility, and promoting tube formation. Through analyses based on two Protein-Protein Interaction databases, Mentha and BioGrid, we identified CUL5 as a protein that might interact with NAMPT, which was then validated by Co-IP experiments. Through interacting with NAMPT, CUL5 inhibited NAMPT expression. In contrast to NAMPT, CUL5 overexpression further aggravated H2O2-induced HCAEC dysfunction. In the meantime, CUL5 overexpression reduced, whereas NAMPT overexpression increased the phosphorylation of p38 and Akt and the protein levels of VEGF and MMP2. More importantly, NAMPT overexpression partially reversed the effects of CUL5 overexpression on H2O2-stimulated HCAECs and the MAPK/PI3K-Akt/VEGF/MMP signaling. In conclusion, CUL5 interacts with NAMPT in H2O2-stimulated HCAECs, suppressing cell viability, promoting cell apoptosis, and inhibiting cell mobility and tube formation. NAMPT overexpression protects against H2O2-induced HCAEC dysfunction by promoting cell viability, inhibiting cell apoptosis, and enhancing cell mobility and tube formation.

2.
J Card Surg ; 36(10): 3554-3560, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34292632

RESUMO

PURPOSE: The role of extracorporeal membrane oxygenatio (ECMO) for rescue therapy of respiratory failure in critically ill coronavirus disease 2019 (COVID-19) patients remains controversial. We aimed to evaluate the clinical outcomes of ECMO in the treatment of COVID-19 compared with conventional ventilation support. METHODS: In this retrospective cohort study, data were collected on extremely critical patients with COVID-19 from January 2020 to March 2020 in intensive care unit of a hospital in charge by national rescue team in Wuhan, China, the epicenter of pandemic. Patients were classified into the ECMO group and the conventional ventilation non-ECMO group. Clinical characteristics, technical characteristics, laboratory results, mortality, and complications of the two groups were analyzed. RESULTS: 88 patients with extremely critical COVID-19 were screened; 34 received ECMO support and 31 received conventional ventilation support. Both groups had comparable characteristics at baseline in terms of age, gender, and comorbidities. Before ECMO or conventional therapy, patients in the two groups had sever acute respiratory distress syndrome with a mean partial pressure of arterial oxygen to the fraction of inspired oxygen (PaO2 /FiO2 ) ratio of 69.6 and 75.4, respectively. At the time of reporting, patients in the ECMO had significantly lower in-hospital mortality compared with the control group (58.8 vs. 93.5%, p = .001). CONCLUSION: ECMO is shown to decrease the mortality of extremely critical ill COVID-19 patients compared with the conventional treatment. Although complications occurred frequently, ECMO could still be a rescue therapy for the treatment of COVID-19 during the pandemic.


Assuntos
COVID-19 , Oxigenação por Membrana Extracorpórea , Estado Terminal , Humanos , Estudos Retrospectivos , SARS-CoV-2
3.
J Int Med Res ; 49(5): 3000605211013176, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33990145

RESUMO

OBJECTIVE: To investigate the effect of focused ultrasonography on clinical outcomes of septic shock. METHODS: Patients with septic shock were randomized into an integrated cardiopulmonary ultrasonography (ICUS) group and conventional (CON) group. Within 1 hour of admission, the ICUS group underwent ICUS examination for hemodynamic decision-making, while the CON group received standard treatment. The primary endpoint was 28-day mortality after admission. The secondary endpoints were cumulative fluid administration in the first 6, 24, and 72 hours; use of vasoactive drugs; lactate clearance; duration of ventilation; and ICU stay. RESULTS: Ninety-four qualified patients were enrolled (ICUS group, 49; CON group, 45). ICUS showed no significant effect on 28-day mortality. Within the initial 6 hours, the ICUS group tended to have a higher fluid balance and fluid intake than the CON group. The duration of vasopressor support was shorter in the ICUS group. There were no differences in the cumulative fluid infusion within 24 or 72 hours, lactate clearance, ICU stay, or duration of ventilation. CONCLUSIONS: The initially focused ICUS did not affect the clinical outcomes of septic shock, but it tended to be associated with a higher fluid balance within the initial 6 hours and shorter duration of vasopressor support.


Assuntos
Choque Séptico , Hidratação , Hemodinâmica , Humanos , Choque Séptico/diagnóstico por imagem , Choque Séptico/tratamento farmacológico , Ultrassonografia , Vasoconstritores/uso terapêutico
4.
Front Public Health ; 9: 603273, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33748059

RESUMO

Background: Since the coronavirus disease-2019 (COVID-19) outbreak, intensive care unit (ICU) healthcare workers were responsible for the critical infected patients. However, few studies focused on the mental health of ICU healthcare workers. This study aimed to investigate the psychological impact of COVID-19 on ICU healthcare workers in China. Methods: We distributed the nine-item Patient Health Questionnaire (PHQ-9) and seven-item General Anxiety Disorder questionnaire (GAD-7) online to ICU healthcare workers in China. Respondents were divided into frontline and second-line according to whether they have contact with COVID-19 patients. Depressive and anxiety symptoms of all respondents were evaluated based on their questionnaire scores. Results: There were 731 ICU healthcare workers finally enrolled in our study, including 303 (41.5%) male, 383 (52.4%) doctors, and 617 (84.4%) aged 26-45 years. All in all, 482 (65.9%) ICU healthcare workers reported symptoms of depression, while 429 (58.7%) reported anxiety. There was no significant difference between frontline (n = 325) and second-line (n = 406) respondents in depression (P = 0.15) and anxiety severity (P = 0.56). Logistic regression analysis showed that being female, ICU work time >5 years, and night duty number ≥10 were risk factors of developing depressive and anxiety symptoms. Income reduction was separately identified as risk of anxiety. Additionally, ICU work time >5 years was also identified as risk of developing moderate-severe depressive and anxiety symptoms. Conclusions: Frontline ICU work was not associated with higher risk of depressive and anxiety symptoms during COVID-19 pandemic remission period in China. Actions like controlling night duty number, ensuring vacation, and increasing income should be taken to relieve mental health problem. Furthermore, we should pay close attention to those who had worked long years in ICU.


Assuntos
Ansiedade/epidemiologia , COVID-19 , Depressão/epidemiologia , Pessoal de Saúde/estatística & dados numéricos , Unidades de Terapia Intensiva , Questionário de Saúde do Paciente/estatística & dados numéricos , Adulto , China/epidemiologia , Estudos Transversais , Feminino , Pessoal de Saúde/psicologia , Humanos , Masculino , Inquéritos e Questionários , Fatores de Tempo
5.
Clin EEG Neurosci ; : 1550059420984934, 2021 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-33405972

RESUMO

OBJECTIVE: This study aimed to explore the effectiveness of quantitative electroencephalogram (EEG) and EEG reactivity (EEG-R) to predict the prognosis of patients with severe traumatic brain injury. METHODS: This was a prospective observational study on severe traumatic brain injury. Quantitative EEG monitoring was performed for 8 to 12 hours within 14 days of onset. The EEG-R was tested during the monitoring period. We then followed patients for 3 months to determine their level of consciousness. The Glasgow Outcome Scale (GOS) score was used. The score 3, 4, 5 of GOS were defined good prognosis, and score 1 and 2 as poor prognosis. Univariate and multivariate analyses were employed to assess the association of predictors with poor prognosis. RESULTS: A total of 56 patients were included in the study. Thirty-two patients (57.1%) awoke (good prognosis) in 3 months after the onset. Twenty-four patients (42.9%) did not awake (poor prognosis), including 11 cases deaths. Univariate analysis showed that Glasgow coma scale (GCS) score, the amplitude-integrated EEG (aEEG), the relative band power (RBP), the relative alpha variability (RAV), the spectral entropy (SE), and EEG-R reached significant difference between the poor-prognosis and good-prognosis groups. However, age, gender, and pupillary light reflex did not correlate significantly with poor prognosis. Furthermore, multivariate logistic regression analysis showed that only RAV and EEG-R were significant independent predictors of poor prognosis, and the prognostic model containing these 2 variables yielded a predictive performance with an area under the curve of 0.882. CONCLUSIONS: Quantitative EEG and EEG-R may be used to assess the prognosis of patients with severe traumatic brain injury early. RAV and EEG-R were the good predictive indicators of poor prognosis.

6.
Zhongguo Dang Dai Er Ke Za Zhi ; 22(12): 1326-1330, 2020 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-33328005

RESUMO

A boy, aged 6 years and 11 months, was admitted due to nephrotic syndrome for 2 years, cough for 1 month, and shortness of breath for 15 days. The boy had a history of treatment with hormone and immunosuppressant. Chest CT after the onset of cough and shortness of breath showed diffuse ground-glass opacities in both lungs. Serum (1, 3)-beta-D glucan was tested positive, and the nucleic acid of cytomegalovirus was detected in respiratory secretions. After the anti-fungal and anti-viral treatment, the child improved temporarily but worsened again within a short period of time. Pneumocystis jirovecii was identified by Gomori's methenamine silver staining in bronchoalveolar lavage fluid. The child was diagnosed with severe pneumonia (Pneumocystis jirovecii and cytomegalovirus infection), acute respiratory distress syndrome, and nephrotic syndrome. After anti-infective therapy with sulfamethoxazole/trimethoprim and ganciclovir and respiratory support, the child still experienced progressive aggravation of dyspnea and tension pneumothorax, and extracorporeal membrane oxygenation (ECMO) was given on day 13 of invasive ventilation. Anti-infective therapy with sulfamethoxazole/trimethoprim, ganciclovir, and linezolid, anticoagulation therapy, sedation therapy, nutrition, and comprehensive management of the respiratory tract were given during ECMO. The child was successfully weaned from ECMO after 72 days, resulting in a length of hospital stay of 134 days. The child was followed up for 6 months after discharge, and there was a significant improvement on lung CT, without organ dysfunction. It is concluded that Pneumocystis jirovecii pneumonia is a potential lifethreatening infection for children with low immunity, and that ECMO can effectively improve the prognosis of children with severe respiratory distress syndrome.


Assuntos
Tosse/etiologia , Infecções por Citomegalovirus/diagnóstico , Dispneia/etiologia , Síndrome Nefrótica , Pneumonia por Pneumocystis/diagnóstico , Síndrome do Desconforto Respiratório/diagnóstico , Criança , Infecções por Citomegalovirus/terapia , Oxigenação por Membrana Extracorpórea , Humanos , Masculino , Síndrome Nefrótica/complicações , Pneumonia por Pneumocystis/terapia , Síndrome do Desconforto Respiratório/terapia
7.
Sci Rep ; 10(1): 13434, 2020 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-32778746

RESUMO

The understanding of water adsorption and desorption behavior in the shale rocks is of great significance in the reserve estimation, wellbore stability and hydrocarbon extraction in the shale gas reservoirs. However, the water sorption behavior in the shales remains unclear. In this study, water vapor adsorption/desorption isotherms of the Longmaxi shale in the Sichuan Basin, China were conducted at various temperatures (30 °C, 60 °C) and a relative pressure up to 0.97 to understand the water sorption behavior. Then the effects of temperature and shale properties were analyzed, and the water adsorption, hysteresis, saturation and capillary pressure were discussed. The results indicate that water adsorption isotherms of the Longmaxi shale exhibit the type II characteristics. The water molecules initially adsorb on the shale particle/pore surfaces at low relative pressure while the capillary condensation dominates at high relative pressure. Temperature favors the water sorption in the shales at high relative pressure, and the GAB isotherm model is found to be suitable for describe the water adsorption/desorption behavior. The high organic carbon and full bedding are beneficial to water adsorption in the shales while the calcite inhibits the behavior. There exists the hysteresis between water adsorption and desorption at the whole relative pressure, which suggests that the depletion of condensed water from smaller capillary pores is more difficult than that from larger pores, and the chemical interaction contributes to the hysteresis loop for water sorption. The capillary pressure in the shales can be up to the order of several hundreds of MPa, and thus the desorption of water from the shales may not be as easy as the water adsorption due to the high capillary pressure, which results in water retention behavior in the shale gas reservoirs. These results can provide insights into a better understanding of water sorption behavior in the shale so as to optimize extraction conditions and predict gas productivity in the shale gas reservoirs.

8.
Nat Prod Res ; : 1-7, 2020 Aug 18.
Artigo em Inglês | MEDLINE | ID: mdl-32811200

RESUMO

One new 14-membered ring cyclopeptide alkaloid, plenane A (1), along with six known compounds (2-7), was isolated from the roots of Clematis florida. Their structures were elucidated by means of NMR spectroscopic analysis and mass spectrometry. In addition, their anti- inflammatory effects on lipopolysaccharide-induced RAW264.7 macrophages were evaluated in vitro. The compounds 1, 2 and 6 exhibited potent indirect inhibitory effects, with IC50 values of 40.92, 22.88 and 6.32 µM, respectively.

9.
Nat Prod Res ; : 1-5, 2020 Jun 11.
Artigo em Inglês | MEDLINE | ID: mdl-32524853

RESUMO

Three new phomalone derivatives, phomalichenones E-G (1-3), and seven known analogues (4-10) were isolated from the cultures of a deep-sea-derived fungus Alternaria sp. MCCC 3A00467. Their structures were elucidated by spectroscopic methods, including the 1D and 2D NMR, and ECD spectrum. Among the compounds isolated, phomalichenone F (2) presented cytotoxic activity against human myeloma cancer U266 cells with IC50 value of 24.99 µg/mL. The most active compound, 10, showed cytotoxicity against U266, HepG2 and A549 cells with IC50 values of 13.26, 14.69 and 24.39 µg/mL, respectively.

10.
Aging (Albany NY) ; 12(4): 3249-3265, 2020 02 15.
Artigo em Inglês | MEDLINE | ID: mdl-32065780

RESUMO

OBJECTIVE: The high mortality rate associated with acute respiratory distress syndrome (ARDS) is a major challenge for intensive care units. In the present study, we applied bioinformatics and animal models to identify core genes and potential corresponding pathways in ARDS. RESULTS: Using bioinformatics analysis, IL-1ß was identified as the core gene of ARDS. Cell experiments showed that up-regulation of IL-1ß downregulates claudin18 to promote lung barrier function damage by regulating the IL-1ß-HER2/HER3 axis, further promoting the development of ARDS. This was validated in the animal models. CONCLUSION: IL-1ß promotes the development of ARDS by regulating the IL-1ß-HER2/HER3 axis. These findings deepen the understanding of the pathological mechanisms of ARDS. METHODS: Transcription data sets related to ARDS were subjected to differential expression gene analysis, functional enrichment analysis, and receiver operating characteristic curve analysis and, so as to identify core genes in ARDS. Cell experiments were used to further explore the effects of core genes on lung barrier function damage. Animal models were applied to validate the effects of core gene in mediating biological signal pathways in ARDS.


Assuntos
Claudina-4/genética , Claudinas/genética , Interleucina-1beta/genética , Pulmão/patologia , Receptor ErbB-2/genética , Síndrome do Desconforto Respiratório/genética , Animais , Claudina-4/metabolismo , Claudinas/metabolismo , Biologia Computacional , Modelos Animais de Doenças , Humanos , Interleucina-1beta/metabolismo , Pulmão/metabolismo , Coelhos , Receptor ErbB-2/metabolismo , Síndrome do Desconforto Respiratório/metabolismo , Síndrome do Desconforto Respiratório/patologia , Transdução de Sinais/fisiologia , Regulação para Cima
11.
Nat Prod Res ; 34(21): 3094-3100, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31230492

RESUMO

A new demethyl abietane diterpenoid, Triptotin K (3) together with three known compounds, friedelin (1), canophyllal (2), and triptonoterpene (4) were isolated from the roots of Tripterygium wilfordii Hook. f. by silica gel column and preparative high performance liquid chromatography. Their structures were determined by extensive NMR data and mass spectroscopic analysis. Triptotin K showed cytotoxic activities against KB, KBv200, HepG2, and MCF-7/ADM cells lines with IC50 values of 29.88, 36.50, 39.55, and 41.38 µM, respectively.


Assuntos
Abietanos/química , Abietanos/farmacologia , Antineoplásicos Fitogênicos/farmacologia , Tripterygium/química , Abietanos/isolamento & purificação , Antineoplásicos Fitogênicos/química , Ensaios de Seleção de Medicamentos Antitumorais , Células Hep G2 , Humanos , Células MCF-7 , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Raízes de Plantas/química , Triterpenos/isolamento & purificação , Triterpenos/farmacologia
12.
Nat Prod Res ; : 1-4, 2020 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-33930989

RESUMO

Clematis florida is widely used in She Ethnopharmacy in China owing to its significant anti-inflammatory activities. This study aimed to investigate the anti-inflammatory effect of the active fraction of C. florida (CFAF) in an arthritis animal model and its possible mechanism. Pre-inflammatory cytokine levels were examined by ELISA. CFAF can significantly improve the symptoms of arthritis such as paw swelling, arthritic index, and histological condition in AA rat. CFAF can also reduce levels of IL-1ß, TNF-α and IL-6. Further studies showed that triterpene saponins from CFAF induced anti-inflammatory activity inhibited inflammatory mediators by blocking JAK/STAT signalling pathways in the LPS-treated macrophages.

13.
Braz. J. Pharm. Sci. (Online) ; 56: e17542, 2020. tab, graf
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1089205

RESUMO

Hepatocellular carcinoma is one of the most prevalent malignancies and a leading cause of cancer-related mortality worldwide. However, the therapies to prevent hepatocellular carcinoma are still limited and the emergence of drug resistance leads to the development of new anti-cancer drugs and combinational chemotherapy regimens. Our study was aimed to explore the anticancer effects of the essential oil extract (EEEO) from Euphorbia esula which has been widely used in traditional Chinese folk medicine and possessed potential cytotoxic effects in several human tumor cells. However, the mechanisms of EEEO-induced anti-proliferation and apoptosis have not been completely elucidated. In this study, EEEO was prepared by hydro-distillation and the main chemical component of EEEO was identified by GC-MS. HepG2 cells were treated with EEEO in vitro and then evaluated with respect to proliferation, apoptosis, and levels of reactive oxygen species (ROS) and apoptotic proteins. Our studies showed that EEEO decreased cell viability, elevated ROS levels, and induced apoptosis of HepG2 cells in a concentration- and time-dependent manner. Furthermore, Bcl-2 was down-regulated, while Bax was up-regulated in HepG2 after EEEO treatment. These results suggest that EEEO induced apoptosis of HepG2 cells and indicate that this apoptosis might be mediated by the mitochondrial pathway.

14.
J Environ Radioact ; 208-209: 105981, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31212250

RESUMO

Some new results were obtained by the array of EN-detectors (Electron and Neutron detectors) developed in the frame of the PRISMA (PRImary Spectrum Measurement Array) project for Extensive Air Showers detection. Our EN-detectors running both on the Earth surface and underground are continuously measuring the environmental thermal neutron flux. Neutrons are partially produced by radioactive gas radon and its daughter decays through (α,n)-reactions in soil close to the detectors. Then neutrons thermalize in media and, being in equilibrium with it, they are sensitive to many geo-dynamic phenomena including earthquakes. In this work the EN-detectors were measuring the variations of an environmental neutron flux in Tibet (30.11 N, 90.53 E, 4300 m a.s.l) at a distance of ∼600 km from the collision zone of the Asian-Indian plates subduction zone (Nepal region). We have observed some anomalies in the dynamics of the neutron flux around the time of the catastrophic earthquakes of magnitude M = 7.8 happened in Gorkha (Nepal) on 25.04.2015 followed by a series of aftershocks of M > 6. The use of nuclear physics methods can provide novel results in geophysics and this work demonstrates the sensitivity of the environmental thermal neutron flux to changes in tense-deformed crust conditions caused by earthquakes with epicentral distances greater than 500 km.


Assuntos
Terremotos , Nêutrons , Monitoramento de Radiação/métodos , Elétrons , Gases , Radônio/análise , Solo , Tibet
15.
Radiat Prot Dosimetry ; 184(3-4): 453-456, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31038699

RESUMO

Accurate measurement of radon progeny concentration is important for the dose assessment of radon exposure and the study of radon progeny behaviours. For measuring 218Po, 214Pb and 214Bi concentration as well as EEC with high sensitivity, an alpha-beta spectrometry method was developed and applied in a step-advanced filter radon progeny monitor. The derivation details of this method is given in this paper and the uncertainty is discussed. The comparison experiments are carried out in radon chamber and in field. Results show that the alpha-beta spectrometry method can give 218Po, 214Pb and 214Bi concentration as well as EEC with high sensitivity either for 60 min or for 30 min cycle, which leads to low uncertainty. This method can be used as a reference method for radon chamber and is suitable for portable radon progeny monitor.


Assuntos
Poluentes Radioativos do Ar/análise , Bismuto/análise , Radioisótopos de Chumbo/análise , Polônio/análise , Monitoramento de Radiação/métodos , Produtos de Decaimento de Radônio/análise , Espectrometria gama/métodos , Partículas alfa , Partículas beta
16.
J Cell Biochem ; 120(9): 14518-14530, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31026096

RESUMO

Endothelial progenitor cells (EPCs) have been reported to replace the damaged endothelial cells to repair the injured or dead endothelium. However, EPC senescence might lead to the failure in EPC function. Thus, developing an in-depth understanding of the mechanism of EPC senescence might provide novel strategies for related vascular disorders' treatments. Herein, nicotinamide phosphoribosyltransferase (NAMPT) overexpression could increase cell proliferation and suppress cell senescence in EPCs. miR-223 directly bound to the 3'-untranslated region of NAMPT to inhibit its expression, therefore modulating EPC proliferation and senescence through NAMPT and phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) signaling. Long noncoding RNA (lncRNA) GAS5 sponges miR-223, consequently downregulating miR-223 expression. GAS5 knockdown inhibited EPC proliferation and promoted senescence. GAS5 might serve as a competing endogenous RNA for miR-223 to counteract miR-223-mediated suppression on NAMPT, thus regulating EPC proliferation and senescence via the PI3K/AKT signaling pathway. In summary, our findings provide a solid experimental basis for understanding the role and mechanism of lncRNA GAS5/miR-223/NAMPT axis in EPC proliferation and senescence.


Assuntos
Citocinas/genética , Células Progenitoras Endoteliais/citologia , MicroRNAs/genética , Nicotinamida Fosforribosiltransferase/genética , RNA Longo não Codificante/genética , Regiões 3' não Traduzidas , Linhagem Celular , Proliferação de Células , Senescência Celular , Células Progenitoras Endoteliais/metabolismo , Humanos , Fosfatidilinositol 3-Quinases/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Transdução de Sinais
17.
Nat Prod Res ; 33(20): 2925-2931, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30518257

RESUMO

One new indole-type alkaloid, α-L-rhamnopyranosyl-(1→6)-ß-D- glucopyranosyl 6-methoxy-3-indolecarbonate (1), together with three known alkaloids (2-4), one aromatic acid (5) and five known saponins (6-10), was isolated from the roots of Clematis florida var. plena. Their structures were established by NMR spectroscopic analysis and acid hydrolysis. In in vivo anti-inflammatory activity, n-butanol extract was found to be potent against ear edema in mice, with inhibition rate of 48.7% at a dose of 800 mg/kg. Furthermore, compounds 8 and 9 obtained from the n-butanol extract exhibited significant anti-inflammatory activities with inhibition rates of 50.9% and 54.7% at a dose of 200 mg/kg.


Assuntos
Alcaloides/isolamento & purificação , Anti-Inflamatórios/isolamento & purificação , Clematis/química , Raízes de Plantas/química , Alcaloides/análise , Animais , Edema/etiologia , Florida , Hidrólise , Indóis/isolamento & purificação , Espectroscopia de Ressonância Magnética , Camundongos , Estrutura Molecular , Extratos Vegetais/farmacologia , Saponinas/química , Triterpenos/química
18.
Nat Prod Res ; 33(3): 386-392, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29569484

RESUMO

Two new phenylspirodrimanes, stachybotrin H (1) and stachybotrysin H (9) together with eleven known analogues (2-8, 10-13) were isolated from deep-sea derived Stachybotrys sp. MCCC 3A00409. Their structures were determined by extensive NMR data and mass spectroscopic analysis. Compounds 9-12 showed weak cytotoxicity against three human cancer cell lines K562, Hela and HL60 with IC50 in the range of 18.5-52.8 µM.


Assuntos
Stachybotrys/química , Antineoplásicos/química , Antineoplásicos/isolamento & purificação , Linhagem Celular Tumoral , Humanos , Estrutura Molecular , Análise Espectral , Compostos de Espiro/química , Compostos de Espiro/isolamento & purificação
19.
Front Pharmacol ; 9: 483, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29867489

RESUMO

Eupatorium adenophorum Spreng. (EA) is a well-known noxious invasive species. Gas chromatography-mass spectrometry (GC-MS) analysis revealed that the essential oil derived from EA (EAEO) is mainly composed of sesquiterpenes. However, the pharmacological value of EAEO in hepatocellular carcinoma (HCC) remains largely unexplored. Herein, we investigated the anti-HCC activities of EAEO, and explored the potential mechanisms of EAEO-induced apoptosis. An MTT assay showed that EAEO inhibited HCC cell proliferation with little toxicity on normal liver cells. Wound healing and FACS assays revealed that EAEO suppressed HCC cell migration and arrested cell cycle, respectively. Moreover, EAEO promoted in vitro HCC cell apoptosis, and EAEO treatment inhibited HepG2 xenografts growth and enhanced apoptotic nucleus of xenografts in HepG2-bearing nude mice. Mechanistically, EAEO significantly decreased the ratio of Bcl-2/Bax and resulted in the activation of caspase-9 and -3. EAEO also reduced the expression of Grp78, which in turn relieved the inhibition of caspase-12 and -7. Meanwhile, EAEO suppressed the phosphorylation of STAT3 and AKT, indicative of its anti-HCC potential. In summary, we determined that EAEO treatment promoted HCC apoptosis via activation of the apoptotic signaling pathway in mitochondria and endoplasmic reticulum, as well as repressed the activity of STAT3 and AKT in HCC cells.

20.
Zhongguo Ying Yong Sheng Li Xue Za Zhi ; 34(2): 187-192, 2018 Feb 08.
Artigo em Chinês | MEDLINE | ID: mdl-29926688

RESUMO

OBJECTIVES: To set up ELISA for detection of atrazine with high precision. METHODS: The reaction condition of indirect-ELISA was optimized, including atrazine-ovalbumin(AT-OVA) concentration and primary antibody concentration, organic solvent, goat anti-rat immunoglobin G-horseradish peroxidase(IgG-HRP) concentration. The actual samples were detected by the ELISA method established in our laboratory. Then the ELISA method was compared with the HPLC. RESULTS: The specification curve of indirect-ELISA was set up after optimization. The relation coefficient R2=0.9958. The limit of detection (LOD) was 1.972 ng/ml. The percent recovery of the actual samples was in range of 80%~120%. The ELISA detection sensitivity was higher than the HPLC in the range of 0 ng/ml~6 ng/ml atrazine. CONCLUSIONS: The ELISA to detect atrazine has good specificity and high precision. And it can be applied in testing real atrazine samples replacing of the large-scale instrument.


Assuntos
Atrazina/análise , Ensaio de Imunoadsorção Enzimática , Animais , Cromatografia Líquida de Alta Pressão , Sensibilidade e Especificidade
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