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1.
Zhongguo Zhong Yao Za Zhi ; 46(12): 3016-3023, 2021 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-34467691

RESUMO

The acupoint application of Euodiae Fructus at Yongquan(KI1) can significantly improve the sleep quality of patients with insomnia with berberine as the main effective component for the efficacy. Nineteen active compounds and 203 drug targets were screened out from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP). After comparison with GeneCards and Online Mendelian Inheritance in Man(OMIM), 24 common genes of diseases and drugs were obtained. STRING 11.0 was used to construct a protein-protein interaction(PPI) network of the overlapping genes, and Matthews correlation coefficient(MCC) was employed to screen the core genes, which were then subjected to enrichment analysis with gene ontology(GO) and Kyoto encyclopedia of genes and genomes(KEGG). The results revealed that the main compounds of Euodiae Fructus, such as berberine and rutaecarpine, participated in the biological processes(such as neurotransmitter receptor activity) by regulating C-reactive protein(CRP), estrogen receptor 1(ESR1), 5-hydroxytryptamine(5-HT) receptor, and interleukin-6(IL-6) to exert sedative, anxiolytic, and antidepressant effects. Sixty 4-week-old SPF mice were randomly divided into a control group, a model group, a positive drug(diazepam tablets) group, and low-, medium-, and high-dose berberine groups. Medication with corresponding drugs was performed for one week. The results demonstrated that berberine was potent in reducing the activities and standing times of mice, down-regulating the levels of CRP and IL-6 mRNA in the hypothalamus, and up-regulating the expression of 5-HT(P<0.01); however, no significant effect on ESR1 was observed. The network of Euodiae Fructus in treating insomnia was constructed by network pharmacology and verified by tests. The findings indicated that the therapeutic efficacy of Euodiae Fructus in treating insomnia was achieved by participating in multiple biological processes, such as neurotransmitter receptor activity, which provided a scientific basis for its clinical application.


Assuntos
Medicamentos de Ervas Chinesas , Distúrbios do Início e da Manutenção do Sono , Animais , Bases de Dados Genéticas , Medicamentos de Ervas Chinesas/farmacologia , Ontologia Genética , Humanos , Medicina Tradicional Chinesa , Camundongos , Distúrbios do Início e da Manutenção do Sono/tratamento farmacológico , Distúrbios do Início e da Manutenção do Sono/genética
3.
Chin Med J (Engl) ; 131(14): 1694-1701, 2018 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-29998889

RESUMO

Background: In the current society, infertility related to age has become a social problem. The in vitro fertilization (IVF) success rate in women with poor ovarian response (POR) is very low. Dandelion extract T-1 (DE-T1) is an effective component of the extract from the leaves and stems of Taraxacum officinale, which is one of the medicines used in some patients with POR, but its molecular mechanism remains unclear. Methods: Following IVF, ovarian granulosa cells (GCs) of sixty patients were extracted and divided into normal ovarian response (NOR) and POR groups. GCs were cultured in a dose-dependent and time-dependent manner with DE-T1, proliferation of GCs was determined by Cell Counting Kit-8 assay, and mRNA levels of insulin-like growth factor 1 receptor (IGF-1R), luteotropic hormone receptor (LHR), follicle-stimulating hormone receptor (FSHR), LHR, and CYP19A1 (aromatase) were determined by quantitative polymerase chain reaction. Progesterone and estradiol (E2) concentrations were determined by enzyme-linked immunosorbent assay. Results: The cell viability gradually increased with the progressive increase in the DE-T1 concentration. Compared with the control group (without DE-T1), the mRNA expressions of FSHR, LHR, IGF-1R, and CYP19A1 were upregulated after the addition of DE-T1, especially in the 2.5% DE-T1 group (P < 0.01). The expression of IGF-1R was upregulated approximately 25 times (24.97 ± 4.02 times) in the POR group with 2.5% DE-T1. E2 and progesterone levels increased with the increasing DE-T1 concentration. There were highly significant differences in the E2 and progesterone secretion between the NOR and POR groups (P < 0.01). Conclusion: DE-T1 may promote steroid hormone synthesis by promoting GC proliferation and upregulating GC receptor expression, thereby improving ovarian endocrine function.


Assuntos
Proliferação de Células/efeitos dos fármacos , Células da Granulosa/metabolismo , Extratos Vegetais/farmacologia , Receptores de Superfície Celular/metabolismo , Taraxacum , Células Cultivadas , Estradiol , Feminino , Hormônio Foliculoestimulante , Células da Granulosa/efeitos dos fármacos , Humanos , Fator de Crescimento Insulin-Like I , Progesterona , Receptores de Superfície Celular/efeitos dos fármacos , Receptores do FSH
4.
J Exp Clin Cancer Res ; 37(1): 80, 2018 Apr 10.
Artigo em Inglês | MEDLINE | ID: mdl-29636108

RESUMO

BACKGROUND: Growth differentiation factor 15 (GDF15) is a member of the TGF-ß superfamily, and evidence suggests that a substantial amount of GDF15 is secreted in various human cancers, such as ovarian cancer, prostate cancer, and breast cancer, among others. However, the function of GDF15 in cervical cancer has not yet been reported. METHODS: Immunohistochemistry was used to detect GDF15 expression in normal cervix and in different cervical cancer lesions. Cell growth curves, MTT, tumor formation assays and flow cytometry were utilized to observe the effects of ectopic GDF15 expression on the proliferation and cell cycle of cervical cancer cells. Real-time PCR, western blotting and immunoprecipitation assays were conducted to measure the expression of genes related to the cell cycle and the PI3K/AKT and MAPK/ERK signaling pathways. A chromatin immunoprecipitation assay was performed to confirm whether C-myc bound to a specific region of the GDF15 promoter. Inhibitor treatment and immunoprecipitation assays were employed to identify the association between GDF15 and ErbB2. RESULTS: GDF15 expression gradually increased during the progression of cervical carcinogenesis. GDF15 promoted cervical cancer cell proliferation via exogenous rhGDF15 treatment or the use of gene editing technology in vitro and in vivo and significantly accelerated the cell cycle transition from G0/G1 to S phase. The expression of p-ErbB2, p-AKT1, p-Erk1/2, CyclinD1 and CyclinE1 was up-regulated and the expression of p21 was down-regulated in GDF15-overexpressing and rhGDF15-treated cervical cancer cells. C-myc trans-activated GDF15 expression by binding to the E-box motifs in the promoter of GDF15 and contributed to the positive feedback of GDF15/C-myc/GDF15. Furthermore, GDF15 bound to ErbB2 in a protein complex in cervical cancer cells. CONCLUSIONS: Our data demonstrated that GDF15 promoted the proliferation of cervical cancer cells via the up-regulation of CyclinD1 and CyclinE1 and the down-regulation of p21 through both the PI3K/AKT and MAPK/ERK signaling pathways in a complex with ErbB2.


Assuntos
Fator 15 de Diferenciação de Crescimento/metabolismo , Proteína Quinase 1 Ativada por Mitógeno/metabolismo , Proteína Quinase 3 Ativada por Mitógeno/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Receptor ErbB-2/metabolismo , Neoplasias do Colo do Útero/metabolismo , Animais , Ciclo Celular , Proteínas de Ciclo Celular/genética , Proteínas de Ciclo Celular/metabolismo , Linhagem Celular Tumoral , Proliferação de Células , Modelos Animais de Doenças , Feminino , Expressão Gênica , Fator 15 de Diferenciação de Crescimento/genética , Xenoenxertos , Humanos , Camundongos , Fosforilação , Ligação Proteica , Transdução de Sinais , Neoplasias do Colo do Útero/genética , Neoplasias do Colo do Útero/patologia
6.
Chin J Integr Med ; 23(5): 345-349, 2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-25877464

RESUMO

OBJECTIVE: To determine the risk factors of the pregnant women with early spontaneous abortion in Beijing. METHODS: A total of 34,417 cases of pregnant women were participated in the survey from January 2000 to December 2013. A questionnaire was informed to each woman. The content of questionnaire includes four parts: general condition, obstetrical history, past history and family history, and living environment and habits. The mental condition was evaluated with Self-Rating Anxiety Scale (SAS) and Self-Rating Depression Scale (SDS). RESULTS: A total of 32,296 questionnaires were collected. The spontaneous abortion rate in the total sample was 3.0%. There was no significant difference between the normal pregnancy group and spontaneous abortion group in terms of general condition, obstetrical and past history (P>0.05). Significant differences between the two groups were found in terms of decoration during pregnancy, keeping pets, near mobile communication base station within 100 m around the residence, drinking during pregnancy, having a cold during pregnancy and SAS (P<0.05). Having a cold during pregnancy, decoration during pregnancy, near mobile communication base station within 100 m around the residence, keeping pets and high SAS were determined the independent risk factors of spontaneous abortion by Logistic regression analysis. CONCLUSIONS: Having a cold during pregnancy, decoration, keeping pets, near mobile communication base station within 100 m around the residence and high SAS are the independent risk factors of spontaneous abortion in Beijing.


Assuntos
Aborto Espontâneo/epidemiologia , Aborto Espontâneo/psicologia , Adolescente , Pequim/epidemiologia , Feminino , Hábitos , Humanos , Saúde Mental , Gravidez , Curva ROC , Fatores de Risco , Inquéritos e Questionários
7.
Pharmazie ; 72(8): 435-439, 2017 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-29441900

RESUMO

Two types of cannabinoid (CB) receptors have been described in the human body: CB1 and CB2 receptors. CB1 receptor distribution may be related to the cannabinoid functions of memory and cognition regulation as well as motor control. In addition, the endocannabinoid system (ECS) related to CB1 receptors may be involved in human emotion regulation, especially depression occurrence. Indeed, CB1 receptors are all distributed in depression associated neuroanatomical structures and neural circuits. Both animal experiments and clinical studies have demonstrated that impairment of the ECS pathway is present in depression models and patients, and application of both CB1 receptor agonists and anandamide (cannabinoid-like substance) degradation inhibitors produce similar biochemical and behavioral effects as antidepressants. These findings provide a solid basis for understanding the ECS role in the formation and development of depression. Therefore, it can be inferred that the ECS may have an important function in both depression treatment and the effects of antidepressants.


Assuntos
Depressão/fisiopatologia , Endocanabinoides/metabolismo , Receptor CB1 de Canabinoide/metabolismo , Animais , Antidepressivos/farmacologia , Ácidos Araquidônicos/farmacologia , Agonistas de Receptores de Canabinoides/farmacologia , Depressão/tratamento farmacológico , Endocanabinoides/farmacologia , Humanos , Alcamidas Poli-Insaturadas/farmacologia , Receptor CB1 de Canabinoide/agonistas
9.
Chin Med J (Engl) ; 129(19): 2331-7, 2016 10 05.
Artigo em Inglês | MEDLINE | ID: mdl-27647193

RESUMO

BACKGROUND: Premature ovarian failure (POF) is a disease that affects female fertility but has few effective treatments. Ovarian reserve function plays an important role in female fertility. Recent studies have reported that hydrogen can protect male fertility. Therefore, we explored the potential protective effect of hydrogen-rich water on ovarian reserve function through a mouse immune POF model. METHODS: To set up immune POF model, fifty female BALB/c mice were randomly divided into four groups: Control (mice consumed normal water, n = 10), hydrogen (mice consumed hydrogen-rich water, n = 10), model (mice were immunized with zona pellucida glycoprotein 3 [ZP3] and consumed normal water, n = 15), and model-hydrogen (mice were immunized with ZP3 and consumed hydrogen-rich water, n = 15) groups. After 5 weeks, mice were sacrificed. Serum anti-Müllerian hormone (AMH) levels, granulosa cell (GC) apoptotic index (AI), B-cell leukemia/lymphoma 2 (Bcl-2), and BCL2-associated X protein (Bax) expression were examined. Analyses were performed using SPSS 17.0 (SPSS Inc., Chicago, IL, USA) software. RESULTS: Immune POF model, model group exhibited markedly reduced serum AMH levels compared with those of the control group (5.41 ± 0.91 ng/ml vs. 16.23 ± 1.97 ng/ml, P = 0.033) and the hydrogen group (19.65 ± 7.82 ng/ml, P = 0.006). The model-hydrogen group displayed significantly higher AMH concentrations compared with that of the model group (15.03 ± 2.75 ng/ml vs. 5.41 ± 0.91 ng/ml, P = 0.021). The GC AI was significantly higher in the model group (21.30 ± 1.74%) than those in the control (7.06 ± 0.27%), hydrogen (5.17 ± 0.41%), and model-hydrogen groups (11.24 ± 0.58%) (all P < 0.001). The GC AI was significantly higher in the model-hydrogen group compared with that of the hydrogen group (11.24 ± 0.58% vs. 5.17 ± 0.41%, P = 0.021). Compared with those of the model group, ovarian tissue Bcl-2 levels increased (2.18 ± 0.30 vs. 3.01 ± 0.33, P = 0.045) and the Bax/Bcl-2 ratio decreased in the model-hydrogen group. CONCLUSIONS: Hydrogen-rich water may improve serum AMH levels and reduce ovarian GC apoptosis in a mouse immune POF model induced by ZP3.


Assuntos
Hidrogênio/farmacologia , Reserva Ovariana/efeitos dos fármacos , Insuficiência Ovariana Primária/metabolismo , Insuficiência Ovariana Primária/prevenção & controle , Água/química , Água/farmacologia , Animais , Hormônio Antimülleriano/sangue , Apoptose/efeitos dos fármacos , Feminino , Células da Granulosa/citologia , Hidrogênio/química , Camundongos , Camundongos Endogâmicos BALB C , Reserva Ovariana/fisiologia , Ovário/efeitos dos fármacos , Ovário/metabolismo , Insuficiência Ovariana Primária/sangue , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Água/administração & dosagem , Zona Pelúcida/efeitos dos fármacos , Zona Pelúcida/fisiologia , Proteína X Associada a bcl-2/metabolismo
10.
Chin Med J (Engl) ; 128(20): 2772-6, 2015 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-26481744

RESUMO

BACKGROUND: Genetic factors are the main cause of early miscarriage. This study aimed to investigate aneuploidy in spontaneous abortion by fluorescence in situ hybridization (FISH) using probes for 13, 16, 18, 21, 22, X and Y chromosomes. METHODS: A total of 840 chorionic samples from spontaneous abortion were collected and examined by FISH. We analyzed the incidence and type of abnormal cases and sex ratio in the samples. We also analyzed the relationship between the rate of aneuploidy and parental age, the rate of aneuploidy between recurrent abortion and sporadic abortion, the difference in incidence of aneuploidy between samples from previous artificial abortion and those from no previous induced abortion. RESULTS: A total of 832 samples were finally analyzed. 368 (44.23%) were abnormal, in which 84.24% (310/368) were aneuploidies and 15.76% (58/368) were polyploidies. The first was trisomy 16 (121/310), followed by trisomy 22, and X monosomy. There was no significant difference in the rate of aneuploidy in the advanced maternal age group (≥ 35 years old) and young maternal age group (<35 years old). However, the rate of trisomy 22 and the total rate of trisomies 21, 13, and 18 (the number of trisomy 21 plus trisomy 13 and trisomy 18 together) showed significantly different in two groups. We found no skewed sex ratio. There was no significant difference in the rate of aneuploidy between recurrent miscarriage and sporadic abortion or between the samples from previous artificial abortion and those from no previous artificial abortion. CONCLUSIONS: Aneuploidy is a principal factor of miscarriage and total parental age is a risk factor. There is no skewed sex ratio in spontaneous abortion. There is also no difference in the rate of aneuploidy between recurrent abortion and sporadic abortion or between previous artificial abortion and no previous induced abortion.


Assuntos
Aborto Espontâneo/genética , Aneuploidia , Aborto Habitual/genética , Adulto , Feminino , Humanos , Hibridização In Situ , Pessoa de Meia-Idade , Gravidez , Razão de Masculinidade
11.
Asian J Androl ; 16(6): 817-23, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24994782

RESUMO

Androgen therapy is the mainstay of treatment for the hypogonadotropic hypogonadal micropenis because it obviously enhances penis growth in prepubescent microphallic patients. However, the molecular mechanisms of androgen treatment leading to penis growth are still largely unknown. To clarify this well-known phenomenon, we successfully generated a castrated male Sprague Dawley rat model at puberty followed by testosterone administration. Interestingly, compared with the control group, testosterone treatment stimulated a dose-dependent increase of penis weight, length, and width in castrated rats accompanied with a dramatic recovery of the pathological changes of the penis. Mechanistically, testosterone administration substantially increased the expression of androgen receptor (AR) protein. Increased AR protein in the penis could subsequently initiate transcription of its target genes, including keratin 33B (Krt33b). Importantly, we demonstrated that KRT33B is generally expressed in the rat penis and that most KRT33B expression is cytoplasmic. Furthermore, AR could directly modulate its expression by binding to a putative androgen response element sequence of the Krt33b promoter. Overall, this study reveals a novel mechanism facilitating penis growth after testosterone treatment in precastrated prepubescent animals, in which androgen enhances the expression of AR protein as well as its target genes, such as Krt33b.


Assuntos
Queratinas/metabolismo , Pênis/crescimento & desenvolvimento , Receptores Androgênicos/metabolismo , Transdução de Sinais , Testosterona/fisiologia , Animais , Sequência de Bases , Primers do DNA , Masculino , Pênis/metabolismo , Ratos , Reação em Cadeia da Polimerase Via Transcriptase Reversa
12.
Asian J Androl ; 16(3): 478-81, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24589466

RESUMO

This study aimed to investigate the correlations among androgen receptor (AR) CAG repeat polymorphism, sex hormones and penile length in healthy Chinese young adult men. Two hundred and fifty-three healthy men (aged 22.8 ± 3.1 years) were enrolled. The individuals were grouped as CAG short (CAG S ) if they harbored repeat length of ≤ 20 or as CAG long (CAG L ) if their CAG repeat length was >20. Body height/weight, penile length and other parameters were examined and recorded by the specified physicians; CAG repeat polymorphism was determined by the polymerase chain reaction (PCR) method; and the serum levels of the sex hormones were detected by radioimmunoassay. Student's t-test or linear regression analysis was used to assess the associations among AR CAG repeat polymorphism, sex hormones and penile length. This investigation showed that the serum total testosterone (T) level was positively associated with the AR CAG repeat length (P = 0.01); whereas, no significant correlation of T or AR CAG repeat polymorphism with the penile length was found (P = 0.593). Interestingly, an inverse association was observed between serum prolactin (PRL) levels and penile length by linear regression analyses (ß= -0.024, P = 0.039, 95% confidence interval (CI): -0.047, 0). Collectively, this study provides the first evidence that serum PRL, but not T or AR CAG repeat polymorphism, is correlated with penile length in the Han adult population from northwestern China.


Assuntos
Hormônios Esteroides Gonadais/sangue , Pênis/anatomia & histologia , Receptores Androgênicos/genética , Repetições de Trinucleotídeos , Adulto , Grupo com Ancestrais do Continente Asiático/genética , China , Humanos , Masculino , Polimorfismo Genético , Prolactina/sangue , Testosterona/sangue , Adulto Jovem
13.
Zhonghua Liu Xing Bing Xue Za Zhi ; 34(6): 557-9, 2013 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-24125602

RESUMO

OBJECTIVE: To understand the epidemiological characteristics of hepatitis C in Henan province. METHODS: Multistage sampling was used to investigate a group of 1-75 year-old general population, living in 30 districts of 18 cities, Henan province. Blood samples were collected and tested for anti-HCV and hepatitis C virus(HCV), from April to July 2012. RESULTS: 32 203 persons were investigated. Among the general population aged 1 to 75 years old, the overall prevalence rates of anti-HCV and HCV RNA were 0.64% and 0.35% respectively. The prevalence rates of anti-HCV among males and females were 0.60%, and 0.68% respectively, with the rates of HCV RNA as 0.37%, and 0.33% respectively. The prevalence rates of anti-HCV and HCV RNA were increasing with age. The prevalence rates of anti-HCV and HCV RNA in urban area were 0.58% and 0.32%, and in rural area as 0.41%, and 0.19% respectively. CONCLUSION: After the implementation of comprehensive measures for HCV control and prevention, Henan province became low prevalent for HCV infection. Our data revealed that the overall prevalence remained low, but the epidemic was severe in some geographical regions in Henan province.


Assuntos
Hepatite C/epidemiologia , Adolescente , Adulto , Idoso , Criança , Pré-Escolar , China/epidemiologia , Feminino , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Fatores de Risco , Inquéritos e Questionários
14.
Asian J Androl ; 15(4): 529-32, 2013 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-23603919

RESUMO

Human papillomavirus (HPV) is one of the most common sexually transmitted diseases which comprises a group of small DNA viruses that infect both cutaneous and mucous squamous epithelia. Liquid bead microarray technology (LBMA) were used to evaluate 24 HPV genotypes in confirmed fertile and infertile males of North China so that the effects of HPV infection on semen parameters and relationship with male infertility could be discussed. A total of 1138 subjects were recruited in this study; 142 were HPV-positive (12.48%). Among 523 confirmed fertile males, only 35 were HPV-positive (6.70%), and two of them had multiple infections. Among 615 infertile males, 107 were HPV-positive (17.4%), and 29 of them had multiple infections. Infertile males had a relatively high HPV infection rate compared with confirmed fertile males. Sperm progressive motility (PR) and the normal morphology rate were significantly decreased in HPV-positive subjects. HPV-45, HPV-52, HPV-18, HPV-59 and HPV-16 infections were more frequently in infertile males. Hence, HPV infection is closely related to male infertility which will decrease sperm PR and morphology. HPV-45, HPV-52, HPV-18, HPV-59 and HPV-16 infection seems to be major risk factors.


Assuntos
Papillomavirus Humano 16/isolamento & purificação , Papillomavirus Humano 18/isolamento & purificação , Infertilidade Masculina/virologia , Infecções por Papillomavirus/complicações , Espermatozoides/virologia , Adulto , China/epidemiologia , Genótipo , Papillomavirus Humano 16/genética , Papillomavirus Humano 18/genética , Humanos , Infertilidade Masculina/epidemiologia , Masculino , Pessoa de Meia-Idade , Infecções por Papillomavirus/epidemiologia , Fatores de Risco , Adulto Jovem
15.
Zhonghua Liu Xing Bing Xue Za Zhi ; 34(11): 1083-5, 2013 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-24517939

RESUMO

OBJECTIVE: To examine the malnutrition status of infants exposed to HIV in rural areas of Henan province and related risk factors. METHODS: Z scores of weight for age, height for age and weight for height of infants exposed to HIV in rural areas of Henan province born in 2010-2012 were calculated at 1, 3, 6, 9, 12 and 18 months of age. Z scores were used to evaluate the nutrition status of infants under study. Data regarding the pregnant women and infants were collected and analyzed by non-conditional logistic regression model. RESULTS: One hundred fifty-seven infants were surveyed in which 125 were HIV negative and 32 were HIV positive. The incidence rates of underweight, athrepsia and growth retardation of HIV-negative infants were 8.8%, 16.8% and 30.4%, respectively. The incidence rates of underweight, athrepsia and growth retardation among the HIV-positive infants were 15.0%, 30.0% and 41.7%, respectively. Anemia in pregnancy (RR = 2.05, 95% CI:1.15-3.66), low count of CD(4)(+)T leuko-monocyte (RR = 1.43, 95% CI:1.18-1.74), status of complementary feeding before 4 months old (RR = 1.96, 95% CI:1.40-2.74) might serve as the risk factors related to the situation. Infants received antiviral treatment (RR = 0.14, 95% CI:0.02-1.04), normal birth weight (RR = 0.08, 95%CI:0.04-0.15) might serve as the protective factors. CONCLUSION: The malnutrition incidence was high among those infants younger than 18 months exposed to HIV in rural area of Henan province. Essential health services such as antenatal care, artificial feeding and growth monitoring for infants and pregnant infants should be provided to prevent malnutrition among these sub-populations.


Assuntos
Infecções por HIV , Transtornos da Nutrição do Lactente/epidemiologia , Exposição Materna , Peso Corporal , China/epidemiologia , Feminino , Humanos , Lactente , Gravidez , Complicações Infecciosas na Gravidez , Fatores de Risco
16.
Zhonghua Liu Xing Bing Xue Za Zhi ; 33(1): 71-4, 2012 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-22575115

RESUMO

OBJECTIVE: To study the management status of HIV-discordant family in Henan province from 2006 to 2010. METHODS: Data on AIDS infected family from "AIDS Infected Family Follow-up Management and Analysis System" was collected and analyzed to understand the management status of HIV-discordant situation. RESULTS: The sero-conversion rates of HIV-discordant spouse were 1.94/100 person-years in 2006, 1.79/100 person-years in 2007, 0.59/100 person-years in 2008 and 0.41/100 person-years in 2009, showing the yearly decrease. Regarding the number of sero-conversion in the same year, male was significantly higher than female in 2007, but not in the other years. The ratio on the course of sero-conversion which was > 2.5 years was increasing. Frequency of most HIV-discordant couples' sexual activities was 1-4 times/month with consistent use of condoms. CONCLUSION: The population of HIV-discordant spouse in Henan province was stable. Regarding management of those HIV-discordant spouse, the acceptance on the rate of test rate was increasing. The course of sero-conversion was also increasing, with most HIV-discordant couples insist on condom use while the sero-conversion rate of HIV-discordant spouse appeared to be low.


Assuntos
Soropositividade para HIV/epidemiologia , China/epidemiologia , Feminino , Infecções por HIV/epidemiologia , Soronegatividade para HIV , Humanos , Masculino , Cônjuges
17.
Chin Med J (Engl) ; 124(8): 1164-8, 2011 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21542989

RESUMO

BACKGROUND: Almost all reported fluorescence in situ hybridization (FISH) kits for prenatal diagnosis use probes from foreign (non-Chinese) countries. The aim of this study was to analyze the reliability of domestic (Chinese) FISH probe sets to detect aneuploidies of chromosomes 13, 18, 21, X, and Y related to prenatal diagnosis in 4210 cases. METHODS: Cytogenetic karyotyping was carried out as a standard prenatal diagnostic test, and amniotic fluid cell interphase FISH analysis was performed using two sets of probes (centromeric probes for chromosomes 18, X, and Y, and locus-specific probes for chromosomes 13 and 21) provided by GP Medical Technologies, Beijing, China. Then we compared the two results and found the performance characteristics for informative FISH results of aneuploidies by the domestic kit probes. RESULTS: In 4210 cases, 4126 cases generated karyotype results and 133 abnormal karyotypes (including 97 aneuploidies) were found. The FISH results of 98 cases (among them, 31 cases gave normal cytogenetic results) were uninformative. The rate of abnormal cases was 3.2% (133/4126). For the abnormal karyotypes, the rate of aneuploidy was 72.9% (97/133). Among the 97 aneuploidies, there were 58 cases of trisomy 21 (58/97, 59.8%), four cases of trisomy 13, 23 cases of trisomy 18, and 12 cases of sex chromosomal aneuploidies. The total concordance of the two methods was 97.9% (95/97; two cases were mosaics that had a low percentage of abnormal cells), and the concordance of trisomy 21, 13, and 18 by the two methods was 100%. CONCLUSIONS: The two sets of the domestic FISH kit probes are reliable for prenatal diagnosis. The results demonstrate that FISH is a rapid and accurate clinical method for prenatal identification of chromosome aneuploidies.


Assuntos
Líquido Amniótico/citologia , Aneuploidia , Hibridização in Situ Fluorescente/métodos , Aberrações Cromossômicas , Feminino , Humanos , Gravidez
18.
Chin Med J (Engl) ; 123(16): 2176-80, 2010 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-20819660

RESUMO

BACKGROUND: For patients with severe endometriosis, the spontaneous pregnancy rates have been reported to be near 0 due to extreme distortion of normal pelvic anatomy. Surgery is one of the treatment options; however, if patients failed to conceive after surgery, in vitro fertilization (IVF) is effective. The objective of this retrospective study was to determine the clinical characteristics of IVF/intracytoplasmic sperm injection (ICSI) in patients with stage III/IV endometriosis, and to determine the impact of the interval from surgery to IVF/ICSI on outcome. METHODS: One hundred and sixty patients who were diagnosed with stage III/IV endometriosis underwent IVF/ICSI cycles between February 2004 and June 2009 were enrolled. The mean interval from surgery to IVF, number of oocytes retrieved, fertilization rate, implantation rate, embryos transferred, and good embryos transferred were compared between two age groups (35 years). RESULTS: The mean interval from surgery to IVF was (37.9+/-28.9) months for the group35 years of age. Twenty-five IVF/ICSI cycles (12.8%) were performed during the first year after surgery, and 34.9% IVF/ICSI cycles were performed 2 years after surgery. No significant differences existed between the two groups with respect to the fertilization rate, implantation rate, number of embryos transferred, number of good embryos, clinical pregnancy rates, live birth rates, and cumulative clinical pregnancy rates (P>0.05). The probability of cumulative clinical pregnancies was 75%, 50%, and 25% ((29.0+/-4.8), (61.0+/-7.6), and (120.0+/-16.9) months after surgery, respectively). CONCLUSIONS: For infertile patients with stage III/IV endometriosis, the optimal time to conceive by IVF/ICSI is <2 years after surgery; nevertheless, most of the patients took a longer time to conceive.


Assuntos
Endometriose/cirurgia , Injeções de Esperma Intracitoplásmicas , Adulto , Feminino , Humanos , Gravidez , Taxa de Gravidez , Estudos Retrospectivos , Fatores de Tempo
19.
Chin Med J (Engl) ; 123(16): 2185-9, 2010 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-20819662

RESUMO

BACKGROUND: Polycystic ovary syndrome (PCOS) is considered to be the most common endocrine disorder in women of reproductive age. The involvement of an abnormal menstrual cycle in the etiology of PCOS remains unclear. We aimed to analyze the characteristics of abnormal menstrual cycles and their association with PCOS in community and hospital patient populations. METHODS: Women with PCOS identified from 2111 permanent female residents in the community of Beijing and 506 outpatients obtained from the reproductive clinic of Peking University Third Hospital were recruited for this study, comprising the PCOS community group and the PCOS hospital group, respectively. Each group was further divided into four subgroups according to the length of menstrual cycles: <21 days; 21-34 days; 35-60 days; and >60 days. Women in each group were interviewed using a questionnaire to assess factors including age, age of menarche, menstrual cycle history, related family history, and modified Ferriman-Gallwey (mF-G) score. All women received transvaginal ultrasound scan and had fasting blood samples taken for endocrine evaluation. A two-tailed P value of <0.05 was considered significant. RESULTS: In the community population, the prevalence of abnormal menstrual cycle was 27.19% (574/2111). The prevalence of PCOS in the community was 6.11% (129/2111) according to Rotterdam criteria. In the community group, the most common menstrual cycle length was 35-60 days, whereas for the hospital group, it was >60 days. In both the community and hospital groups, the most common phenotype of PCOS was that of Oligo/amenorrhea+PCO+ hyperandrogenism (HA) (O+P+H) (P=0.000). With increasing cycle length of 35-60 days to >60 days, the percentages of Oligo/amenorrhea+PCO (O+P) and O+P+H were found to significantly decrease in the community group and significantly increase in the hospital group (P=0.000 for each). In the hospital group, as the menstrual cycle length increased from 35-60 days to >60 days, the rate of spontaneous abortion increased significantly (P=0.000), meanwhile the rate of poorly-secreted endometrium and abnormal endometrial hyperplasia increased significantly (P=0.000). CONCLUSIONS: The prevalence of PCOS in the Beijing community of women was 6.11%. Oligo/amenorrhea was the most common type of abnormal menstrual cycle and may be an indicator for PCOS and endometrial lesions. Gynecologists should seek relevant medical information from women in the community to promptly diagnose PCOS and then follow up patients for potential development of subsequent complications.


Assuntos
Ciclo Menstrual/fisiologia , Síndrome do Ovário Policístico/diagnóstico , Adulto , Hiperplasia Endometrial/diagnóstico , Hiperplasia Endometrial/fisiopatologia , Feminino , Humanos , Pessoa de Meia-Idade , Síndrome do Ovário Policístico/fisiopatologia
20.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao ; 32(3): 340-2, 2010 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-20602892

RESUMO

OBJECTIVE: To evaluate the effect of micronutrients on the immune status of human immunodeficiency virus (HIV)-positive individuals. METHODS: Totally 102 HIV-positive individuals were randomly divided into supplementation group (received micronutrients supplement) and control group (received placebo). Physical examinations were performed at baseline and at the end of the trial. Immune status were determined in both two groups. RESULTS: Age, height, weight, and sex ratio were not significantly different between two groups (all P>0.05). Baseline CD3+, CD4+, and CD8+ T lymphocytes and IgA, IgG, IgM, C3 levels were not significantly different between two groups (all P>0.05), while the levels of CD3+, CD4+, and CD8+ T lymphocytes and IgA, IgG, IgM, C3 were significantly higher in supplementation group than in control group(all P0.05). CONCLUSION: Supplementation of micronutrients can improve the immune status of HIV-positives individuals.


Assuntos
Infecções por HIV/imunologia , Micronutrientes/uso terapêutico , Adulto , Suplementos Nutricionais , Infecções por HIV/tratamento farmacológico , Humanos , Pessoa de Meia-Idade
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