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J Chromatogr A ; 1609: 460510, 2020 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-31515077


In this study, a novel phenyl-boronic acid polymeric monolith (PBAPM) in polyether ether ketone (PEEK) tube was fabricated. The inner wall of PEEK tube was modified with mussel inspired polydopamine layer to firmly bond PBAPM, so as to avoid the outflow of PBAPM from PEEK tube and improve the service life and application scope of PBAPM. The PBAPM was synthesized by initiator-free ring-opening polymerization based on our previous work. The boric acid groups provided B-N coordination sites, as well as the hydrophobic amino and epoxy monomers provided hydrophobic interaction sites. Due to the synergistic effect of hydrophobic interaction and B-N coordination, the PBAPM exhibited excellent binding amounts for nitrogen-containing sulfonamides (SAs). In addition, the PBAPM possessed excellent stability, rigidity and permeability. Therefore, the PBAPM was used as solid phase microextraction (SPME) material for enrichment and separation of SAs from aqueous samples. The PBAPM SPME was optimized in detail, and combined with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) analysis for simultaneous determination of 10 kinds of SAs from tap, lake and river water. Using only 1 mL of water samples, limit of quantitation of SAs could reach 0.54-4.5 ng L-1. Recoveries of standard spiked SAs from water samples were between 82.0% and 105.4%, with intra-day and inter-day relative standard deviation ranging from 3.3% to 5.6% and 4.2% to 8.1%, respectively. The PBAPM SPME combined with UPLC-MS/MS method shown better or similar recoveries, and used fewer samples than previous methods. These results demonstrated that the PBAPM could selectively separate and enrich ultra-trace nitrogen-containing SAs from aqueous samples.

Ácidos Borônicos/química , Cromatografia Líquida de Alta Pressão/métodos , Polimerização , Polímeros/química , Microextração em Fase Sólida , Sulfonamidas/análise , Espectrometria de Massas em Tandem/métodos , Sulfonamidas/química , Água/química , Poluentes Químicos da Água/análise
Appl Microbiol Biotechnol ; 103(11): 4313-4324, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31016357


In recent years, eukaryotic microorganisms have been widely applied to offer many solutions for everyday life and have come to play important roles in agriculture, food, health care, and the fine-chemicals industry. However, the complex genetic background and low homologous recombination efficiency have hampered the implementation of large-scale and high-throughput gene editing in many eukaryotic microorganisms. The low efficiency of homologous recombination (HR) not only makes the modification process labor-intensive but also completely precludes the application of many otherwise very useful genome editing techniques. Thus, increasing the efficiency of HR is clearly an enabling technology for basic research and gene editing in eukaryotic microorganisms. In this review, we summarize the current strategies for enhancing the efficiency of HR in eukaryotic microorganisms (particularly yeasts and filamentous fungi), list some small molecules and candidate genes associated with homologous and non-homologous recombination, and briefly discuss the further development prospects of these strategies.

Fungos/genética , Edição de Genes/métodos , Recombinação Homóloga , Engenharia Metabólica/métodos , Leveduras/genética
Bioresour Technol ; 281: 449-456, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30846235


Terpenoids are a large class of natural compounds based on the C5 isoprene unit, with many biological effects such activity against cancer and allergies, while some also have an agreeable aroma. Consequently, they have received extensive attention in the food, pharmaceutical and cosmetic fields. With the identification and analysis of the underlying natural product synthesis pathways, current microbial-based metabolic engineering approaches have yielded new strategies for the production of highly valuable terpenoids. Yarrowia lipolytica is a non-conventional oleaginous yeast that is rapidly emerging as a valuable host for the production of terpenoids due to its own endogenous mevalonate pathway and high oil production capacity. This review aims to summarize the status and strategies of metabolic engineering for the heterologous synthesis of terpenoids in Y. lipolytica in recent years and proposes new methods aiming towards further improvement of terpenoid production.

Engenharia Metabólica/métodos , Terpenos/metabolismo , Yarrowia/metabolismo , Produtos Biológicos/metabolismo
Sci Rep ; 9(1): 1540, 2019 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-30733513


This study proposes the usage of an effective potential to investigate a dissipative quantum system with rotational velocity. After gauge transformation, a Doebner- Goldin equation (DGE) that describes the dissipative quantum system with a Dirac potential is obtained. The DGE is solved based on constraint of vertical relation between the rotational velocity field and density gradient when a harmonic oscillator model is considered. It is observed that the dissipative quantum system is directly equivalent to a monopole system and that the two gauge potentials that are given by Wu and Yang in the north and south hemispheres can be reproduced. Furthermore, a set of gauge-invariant parameters is obtained to discuss the dissipation characteristics of the system.