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1.
Int J Food Microbiol ; 360: 109325, 2021 Dec 16.
Artigo em Inglês | MEDLINE | ID: mdl-34281717

RESUMO

Catarratto is one of the most common non-aromatic white grape varieties cultivated in Sicily (Southern Italy). In order to improve the aromatic expression of Catarratto wines a trial was undertaken to investigate the effect of yeast strain, nutrition and reduced glutathione. Variables included two Saccharomyces cerevisiae strains, an oenological strain (GR1) and one isolated from honey by-products (SPF52), three different nutrition regimes (Stimula Sauvignon Blanc™ (SS), Stimula Chardonnay™ (SC) and classic nutrition practice), and a specific inactivated yeast rich in reduced glutathione to prevent oxidative processes [Glutastar™ (GIY)] ensuing in ten treatments (T1-T10). Microbiological and chemical parameters demonstrated the aptitude of strain SPF52 to successfully conduct alcoholic fermentation. During fermentation, the Saccharomyces yeast populations ranged from 7 to 8 logarithmic CFU/mL. All wines had a final ethanol content ranging between 12.91 and 13.85% (v/v). The dominance of the two starter strains over native yeast populations was higher than 97% as estimated by interdelta analysis. The addition of nutrients SS or SC increased the aromatic complexity of the wines as reflected by volatile organic compounds (VOCs) composition and sensory profiles. In particular, 32 VOCs were identified; alcohols (62.46-81.1%), thiols (0.27-0.87%), ethers (0.09-0.16%), aldehydes (0-1.21%), ketones (0-2.28%), carboxylic acids (4.21-12.32%), esters (0-10.85%), lactones (0.9-1.49%) and other compounds (0.77-6.9%). Sensory analysis demonstrated a significant impact on wine aroma in relation to yeast starter strain used, the type of nutrition (SS, SC or classic nutrition) and the presence/absence of GIY. The wines produced with GR1 yeast strain and SS (T2), SPF52 with SC (T9) both in presence of GIY showed higher overall quality. Trials T2 and T9 showed the highest scores for 13 and 18 attributes, respectively. The different nutrition, addition of GIY and the yeast starter strains diversified and enhanced sensory expression of Catarratto wines.


Assuntos
Vitis , Compostos Orgânicos Voláteis , Vinho , Fermentação , Glutationa , Nutrientes , Odorantes/análise , Saccharomyces cerevisiae , Sicília , Compostos Orgânicos Voláteis/análise , Vinho/análise
2.
Nat Prod Res ; : 1-6, 2020 Mar 09.
Artigo em Inglês | MEDLINE | ID: mdl-32146851

RESUMO

The hydrolysis of acetyl moieties on a set of commercially relevant substrates was performed by employing the whole tissue of Citrus sinensis (L.) Osbeck peel as an efficient biocatalyst in mild reaction conditions with high degree of regioselectivity. The reaction is done in aqueous media and the product is easily recovered. Optimal reaction conditions were deduced and two practical applications were investigated: the elaboration of acetylglicerols and the preparation of vitamin K1 precursor. Peel waste (flavedo and albedo) from orange juice manufacturing was successfully employed as a biocatalyst.

3.
Nat Prod Res ; 34(15): 2131-2136, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30873863

RESUMO

Abies nebrodensis (Lojac.) Mattei (Pinaceae) is a species living in a very small population only in a confined area of Sicily. In this study, the dichloromethane extract of the leaves was analyzed. Apart from three already known metabolites namely dehydroabietic acid; maltol; and rheosmin, previously detected in other species of Abies, a lanostane derivative was isolated. Its chemical structure was elucidated by means of extensive spectroscopic methods.


Assuntos
Abies/química , Compostos Fitoquímicos/análise , Folhas de Planta/química , Abietanos , Butanonas , Estrutura Molecular , Fenóis , Compostos Fitoquímicos/química , Pinaceae/química , Extratos Vegetais/química , Pironas , Sicília , Análise Espectral
4.
Antioxidants (Basel) ; 8(10)2019 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-31546694

RESUMO

Ethanolic extracts from Mangifera indica L. have been proved to possess anti-tumor properties in many cancer systems. However, although most effects have been demonstrated with fruit pulp extract, the underlying molecular mechanisms of mango peel are still unclear. This study was designed to explore the effects of mango peel extract (MPE) on colon cancer cell lines. MPE affected cell viability and inhibited the colony formation trend of tumor cells, while no effects were observed in human dermal fibroblasts used as a non-cancerous cell line model. These events were a consequence of the induction of apoptosis associated to reactive oxygen species (ROS) production, activation of players of the oxidative response such as JNK and ERK1/2, and the increase in Nrf2 and manganese superoxide dismutase (MnSOD). Significantly, mango peel-activated stress triggered a DNA damage response evidenced by the precocious phosphorylation of histone 2AX (γH2AX), as well as phosphorylated Ataxia telangiectasia-mutated (ATM) kinase and p53 upregulation. Mango peel extract was also characterized, and HPLC/MS (High Performance Liquid Chromatography/Mass Spectrometry) analysis unveiled the presence of some phenolic compounds that could be responsible for the anti-cancer effects. Collectively, these findings point out the importance of the genotoxic stress signaling pathway mediated by γH2AX in targeting colon tumor cells to apoptosis.

5.
Nat Prod Res ; 33(1): 80-88, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29394873

RESUMO

Distillation time can both to optimise the production and to engineer the composition of essential oil in essential oil bearing plants. Purpose of this study was to evaluate the effect of duration of hydrodistillation on composition of essential oil of Thymus × citriodorus, the natural source of commercially important geraniol and citral, a component with valuable biological properties. Essential oils were isolated by hydrodistillation at different distillation times and analysed by GC/MS analytical methods. Increase in percentage of essential oil during all hydrodistillation time gradient was uneven. Elongation of hydrodistillation time decreased percentages of monoterpenes but increased percentages of sesquiterpenes in essential oil. Results showed that the hydrodistillation of essential oil from lemon thyme longer than 60 min is useless.


Assuntos
Destilação/métodos , Óleos Voláteis/química , Thymus (Planta)/química , Monoterpenos Acíclicos , Cromatografia Gasosa-Espectrometria de Massas , Monoterpenos/análise , Sesquiterpenos/análise , Terpenos , Fatores de Tempo
6.
Plant Signal Behav ; 14(1): 1552056, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30507332

RESUMO

Sexually deceptive orchid flowers use visual, tactile and olfactory cues of female insects in order to attract males of one or a few closely related species as pollinators. Ophrys L. is the most species-rich genus of sexually deceptive orchids in the Mediterranean Basin. Despite Ophrys pollinated by Andrena male bees use alkanes and mainly alkenes with specific double-bond positions as key signals that trigger pseudocopulatory behavior, some volatile organic compounds (VOCs) with low molecular weight were found as long-range attractants non-eliciting copulatory behavior. Since floral scents in Ophrys have been extensively studied by solvent extractions here we aimed to understand which floral volatiles are found when two different collection methods are used in Ophrys panormitana flowers. By knowing their chemical composition, we could better understand the scent chemistry of this Ophrys species without overlooking VOCs which could also have a function in its pollination biology. Scent samples collected by dynamic headspace and by solvent extraction were analyzed by gas chromatography/mass spectrometry (GC/MS). The floral scent of O. panormitana is composed by a bouquet of VOCs with lower and higher molecular weights. The headspace samples contained VOCs with higher volatility (mainly one aliphatic alcohol and two aliphatic ketones) whereas the solvent extracts were composed by VOCs with lower volatility (exclusively long-chain alkanes and alkenes). Overlapping in VOCs between headspace and solvent samples were not found. For the first time Andrena nigroaenea was observed during the pseudocopulation and removing the pollinaria of a flower of O. panormitana. Abbreviations: VOCs, volatile organic compounds; GC/MS, gas chromatography-mass spectrometry; KRI, Kovats Retention Indices.


Assuntos
Orchidaceae/metabolismo , Compostos Orgânicos Voláteis/metabolismo , Flores/metabolismo , Cromatografia Gasosa-Espectrometria de Massas , Odorantes , Polinização/fisiologia , Compostos Orgânicos Voláteis/análise
7.
Phytochemistry ; 158: 86-90, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30481663

RESUMO

Iris species can adopt different pollination strategies to attract their pollinators, generalized shelter-mimicking, specialized deceptive sexual-mimicking or food-rewarding. As attractive stimuli, Iris flowers may use their colours, large-size, symmetry, and volatile organic compounds (VOCs). However, relatively few studies investigated Iris floral olfactory cues in the context of plant-visitor/pollinator interactions. In the present study we combined the identification of the floral volatiles of the nectariferous I. planifolia with insects visiting its flowers to gather data on its biology. Floral volatiles were collected in the natural environment by dynamic headspace and analysed by gas chromatography-mass spectrometry (GC-MS). Insect visitors/pollinators were also recorded. The volatile bouquet was aromatic-dominated with 1,4 dimethoxybenzene as major compound. Among the insects visiting its flowers, bumble and honey bees were the most abundant followed by hover flies. Overall, our results suggest that I. planifolia advertises its food reward by an aromatic dominated volatile composition.


Assuntos
Flores/fisiologia , Insetos/fisiologia , Iris (Planta)/fisiologia , Compostos Orgânicos Voláteis/análise , Animais , Anisóis/análise , Abelhas , Flores/química , Cromatografia Gasosa-Espectrometria de Massas , Iris (Planta)/química , Itália , Polinização
8.
Nutrients ; 10(10)2018 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-30322062

RESUMO

Litchi chinensis Sonnerat is a tropical tree whose fruits contain significant amounts of bioactive polyphenols. Litchi cultivation has recently spread in Sicily where the climate conditions are particularly favorable for this crop. Recent findings have shown that Litchi extracts display anti-tumor and pro-apoptotic effects in vitro, but the precise underlying mechanisms have not been fully elucidated. In this study, we report for the first time the effects of Sicilian litchi fruit extracts on colon cancer cells. The results indicated that litchi exocarp, mesocarp and endocarp fractions reduce the viability and clonogenic growth of HT29 cells. These effects were due to cell cycle arrest in the G2/M phase followed by caspase-dependent cell death. Interestingly, litchi exocarp and endocarp triggered a precocious autophagic response (16⁻24 h), which was accompanied by an increase in the level of autophagy related 1/autophagy activating kinase 1 (ATG1/ULK1), beclin-1, microtubule associated protein 1 light chain 3 (LC3)-II and p62 proteins. Autophagy inhibition by bafilomycin A1 or beclin-1 silencing increased cell death, thus suggesting that autophagy was initially triggered as a pro-survival response. Significant effects of Litchi extracts were also observed in other colon cancer cells, including HCT116 and Caco-2 cells. On the other hand, differentiated Caco-2 cells, a model of human enterocytes, appeared to be insensitive to the extracts at the same treatment conditions. High-Performance Liquid Chromatography⁻Electrospray Ionization-Quadrupole-Time-Of-Flight HPLC/ESI/Q-TOF evidenced the presence of some polyphenolic compounds, specifically in exocarp and endocarp extracts, that can account for the observed biological effects. The results obtained suggest a potential therapeutic efficacy of polyphenolic compounds purified from Sicilian Litchi fractions for the treatment of colon cancer. Moreover, our findings indicate that modulation of autophagy can represent a tool to improve the effectiveness of these agents and potentiate the anti-tumor response of colon cancer cells.


Assuntos
Antineoplásicos Fitogênicos/uso terapêutico , Apoptose , Autofagia , Neoplasias do Colo/tratamento farmacológico , Litchi/química , Fitoterapia , Polifenóis/uso terapêutico , Antineoplásicos Fitogênicos/farmacologia , Células CACO-2 , Pontos de Checagem do Ciclo Celular/efeitos dos fármacos , Frutas/química , Células HT29 , Humanos , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico , Polifenóis/farmacologia , Sicília , Transdução de Sinais
9.
Plant Mol Biol ; 98(4-5): 407-425, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30341661

RESUMO

KEY MESSAGE: Our results provide a comprehensive overview how the alloplasmic condition might lead to a significant improvement in citrus plant breeding, developing varieties more adaptable to a wide range of conditions. Citrus cybrids resulting from somatic hybridization hold great potential in plant improvement. They represent effective products resulting from the transfer of organelle-encoded traits into cultivated varieties. In these cases, the plant coordinated array of physiological, biochemical, and molecular functions remains the result of integration among different signals, which derive from the compartmentalized genomes of nucleus, plastids and mitochondria. To dissect the effects of genome rearrangement into cybrids, a multidisciplinary study was conducted on a diploid cybrid (C2N), resulting from a breeding program aimed to improve interesting agronomical traits for lemon, the parental cultivars 'Valencia' sweet orange (V) and 'femminello' lemon (F), and the corresponding somatic allotetraploid hybrid (V + F). In particular, a differential proteomic analysis, based on 2D-DIGE and MS procedures, was carried out on leaf proteomes of C2N, V, F and V + F, using the C2N proteome as pivotal condition. This investigation revealed differentially represented protein patterns that can be associated with genome rearrangement and cell compartment interplay. Interestingly, most of the up-regulated proteins in the cybrid are involved in crucial biological processes such as photosynthesis, energy production and stress tolerance response. The cybrid differential proteome pattern was concomitant with a general increase of leaf gas exchange and content of volatile organic compounds, highlighting a stimulation of specific pathways that can be related to observed plant performances. Our results contribute to a better understanding how the alloplasmic condition might lead to a substantial improvement in plant breeding, opening new opportunities to develop varieties more adaptable to a wide range of conditions.


Assuntos
Núcleo Celular/fisiologia , Citrus sinensis/genética , Citrus/genética , Citoplasma/fisiologia , Diploide , Melhoramento Vegetal/métodos , Núcleo Celular/metabolismo , Cromatografia Líquida de Alta Pressão , Cromatografia de Fase Reversa , Citrus/metabolismo , Citrus sinensis/metabolismo , Citoplasma/metabolismo , Dissacarídeos , Eletroforese em Gel Bidimensional , Glucuronatos , Espectrometria de Massas , Metabolômica/métodos , Folhas de Planta/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/isolamento & purificação , Proteínas de Plantas/metabolismo , Proteômica/métodos , Compostos Orgânicos Voláteis/metabolismo
10.
Foods ; 7(6)2018 Jun 20.
Artigo em Inglês | MEDLINE | ID: mdl-29925768

RESUMO

The aim of this study (first analytical approach) was to obtain data concerning the fatty acid composition of gluten-free foods (bakery products) for celiac people. The study included 35 different products (snacks, biscuits, bakery products, pasta, flours, etc.) from several manufacturers. After extraction and esterification, the fatty acid composition was determined by Gaschromatography (GC⁻MS) Monounsaturated fatty acids (MUFAs) were found to be the major constituents (57%), followed by saturated fatty acids (SFAs) (30%), and polyunsaturated fatty acid (13%). Only 15 of the 35 gluten-free samples analyzed appeared to provide adequate energy intake, while, in 11 samples, saturated fatty acids were found to supply more energy than that recommended by the European Food Safety Authority EFSA. Moreover, data analyses showed that, although gluten-free commercial products are high added-value foods, industrial products in many cases contain palm and palm kernel oils, whereas the local producers generally use the finest raw materials, such as olive oil.

11.
PLoS One ; 13(5): e0196947, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29723282

RESUMO

The biological properties of essential oils have been demonstrated in the treatment of several diseases and to enhance the bioavailability of other drugs. In natural habitats the essential oils compounds may play important roles in the protection of the plants as antibacterials, antivirals, antifungals, insecticides and also against herbivores by reducing their appetite for such plants or by repelling undesirable others. We analyzed by gas-chromatography mass spectrometry the chemical composition of the essential oil of aerial parts of Glandora rosmarinifolia (Ten.) D.C. Thomas obtained by hydrodistillation and verified some biological activities on a panel of hepatocellular carcinoma cell lines (HA22T/VGH, HepG2, Hep3B) and triple negative breast cancer cell lines (SUM 149, MDA-MB-231). In the essential oil we detected 35 compounds. The results of the biological assays indicate that essential oil of G. rosmarinifolia induces cell growth inhibition at concentration-dependent way in all cell line models. This oil does not seem to possess antioxidant activity, while the cytotoxicity of G. rosmarinifolia essential oil appeared to involve, at least in part, a pro-oxidant mechanism. Our results show for the first time the antitumoral and pro-oxidant activities of G. rosmarinifolia essential oil and suggest that it may represent a resource of pharmacologically active compounds.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Boraginaceae/química , Óleos Voláteis/química , Oxidantes/farmacologia , Óleos Vegetais/química , Compostos Orgânicos Voláteis/farmacologia , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/isolamento & purificação , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Relação Dose-Resposta a Droga , Células Epiteliais/efeitos dos fármacos , Células Epiteliais/patologia , Hepatócitos/efeitos dos fármacos , Hepatócitos/patologia , Humanos , Concentração Inibidora 50 , Oxidantes/química , Oxidantes/isolamento & purificação , Componentes Aéreos da Planta/química , Extratos Vegetais/química , Compostos Orgânicos Voláteis/química , Compostos Orgânicos Voláteis/isolamento & purificação
12.
Nat Prod Res ; 31(23): 2759-2767, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-28278620

RESUMO

The chemical composition of the essential oils isolated from the aerial parts of Anthemis arvensis L. subsp. arvensis, Anthemis cretica subsp. messanensis (Brullo) Giardina & Raimondo and from flowers and leaves of Anthemis cretica subsp. columnae (Ten.) Frezén were determinated by GC-FID and GC-MS analyses. Torreyol (85.4%) was recognised as the main constituent of the Anthemis arvensis subsp. arvensis essential oil, while in the essential oils of Anthemis cretica subsp. messanensis, collected on the rock and cultivated in Hortus Botanicus Panormitanus, (E)-chrysanthenyl acetate (28.8 and 24.2% resp.), 14-hydroxy-α-humulene (8.1 and 5.3% resp.), santolina triene (8 and 5.8% resp.) and α-pinene (6.7 and 5.4% resp.) prevailed. 18-cineole (13.3 and 12.2% resp.), was the main component of both flower and leaf oils of Anthemis cretica subsp. columnae together with δ-cadinene (9.0 and 8.2% resp.) and (E)-caryophyllene (8.3 and 5.6% resp.).


Assuntos
Anthemis/química , Anti-Infecciosos/química , Anti-Infecciosos/farmacologia , Óleos Voláteis/química , Monoterpenos Bicíclicos , Compostos Bicíclicos com Pontes/análise , Cicloexanóis/análise , Avaliação Pré-Clínica de Medicamentos/métodos , Eucaliptol , Flores/química , Cromatografia Gasosa-Espectrometria de Massas , Testes de Sensibilidade Microbiana , Monoterpenos/análise , Óleos Voláteis/análise , Folhas de Planta/química , Sesquiterpenos Policíclicos , Sesquiterpenos/análise , Sicília
13.
Chem Biodivers ; 14(2)2017 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-27490979

RESUMO

Abies nebrodensis (Lojac.) Mattei (Pinaceae) is a species occurring in a very small population only in a restricted area of Sicily. Its taxonomic classification as different species has been object of discussion. In this work the chemical composition of the essential oil from the leaves is presented for the first time and compared to the essential oils from other euroasiatic species reported in literature. Peculiar characteristics of the essential oil of A. nebrodensis are highlighted.


Assuntos
Abies/química , Óleos Voláteis/química , Folhas de Planta/química , Óleos Vegetais/química , Cromatografia Gasosa-Espectrometria de Massas
14.
Nat Prod Res ; 31(9): 990-999, 2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-27855490

RESUMO

Globe artichoke has been long considered a nutraceutical food for its valuable content of bioactive compounds. However, beside a well-known polyphenol profile, poor information is available about its metabolite and mineral composition. The aim of this study was to investigate edible parts of Sicilian artichokes, 'Spinoso di Menfi' and 'Violetto di Niscemi', by 1H NMR and ICP-MS for elucidating these compositional aspects. Although bracts and hearts of both artichokes shared a very similar metabolite pattern, 'Spinoso di Menfi' showed a higher number of metabolites, such as amino acids and polyphenols, than 'Violetto di Niscemi'. 'Spinoso di Menfi' was also marked by higher levels of macro- and microelements when compared to 'Violetto di Niscemi'. Also, artichoke heart demonstrated to accumulate higher mineral levels than bracts. 1H NMR and ICP-MS successfully profiled metabolites and metals in such plant food, partially covering the lack of literature data about 'Spinoso di Menfi' and 'Violetto di Niscemi' artichokes.


Assuntos
Cynara scolymus/metabolismo , Espectroscopia de Ressonância Magnética/métodos , Minerais/análise , Espectrofotometria Atômica/métodos , Cynara scolymus/química , Espectrometria de Massas , Polifenóis/análise , Polifenóis/metabolismo
15.
Chem Biodivers ; 13(10): 1357-1368, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27444992

RESUMO

Ocimum basilicum L. (Lamiaceae) is an aromatic plant of great tradition in the Mediterranean area. Its economic importance is growing up determining an expansion of cultivation. This paper evaluated the morphological traits, the chemical profiles, and antibacterial activity of 21 cultivars of basil belonging to 'Genovese', 'Napoletano', and 'Purple basil' types. The cultivars were characterized by different growth rate and morphological traits. The chemical composition of the oils analyzed by GC and GC/MS analysis, supported by the PCA analysis, underlined the strong influence of chemotype. It is noteworthy that estragole, never present in Genovese and purple basil types, occurred in Napoletano type. The high presence of eugenol, methyl eugenol, and linalool in the majority of cultivars, belonging both to Genovese and to Napoletano types was registered. Of great interest resulted the composition of the purple basil 'Opal'. All the samples tested exhibited similar antibiotic profiles with moderate antibacterial activity. The results enhanced the importance of determination of essential-oil profile in the selection of cultivars characterized by diverse morphological traits and are useful for different purposes.


Assuntos
Antibacterianos/farmacologia , Ocimum basilicum/química , Óleos Voláteis/química , Óleos Voláteis/farmacologia , Antibacterianos/análise , Antibacterianos/química , Relação Dose-Resposta a Droga , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Positivas/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Óleos Voláteis/análise , Relação Estrutura-Atividade
16.
Curr Pharm Des ; 22(26): 4011-27, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27281330

RESUMO

BACKGROUND: The use of aromatic plants to relief different illness is not a new therapy. Actually aromatic plants have been used for many centuries by different cultures around the world. Pharmacological studies provide scientific support to the traditional use of aromatic medicinal plants and aromatherapy; nevertheless, more clinical trials are required regarding to their effectiveness in order to establish a guidance for their use in routine healthcare. Moreover, modern medicine in studies about olfactory function has attained great achievements and got Nobel Prize in 2004. These new searches have obviously fueled interest in the essential oils and volatile compounds of natural origin. Several reviews on the newly discovered AChEi obtained from plants, fungus and marine organisms have also been published over the last years. The majority of these AChEi belong to the alkaloid group, including indole, isoquinoline, quinolizidine, piperidine and steroidal alkaloids. RESULTS: Probably the interest in the essential oils and volatile compounds will be fueled from the new available scientific data about receptor on olfactory mucosa of nasal cavity. It can receive and distinguish different odor molecules, which produce nerve impulse and transmit into olfactory bulb via olfactory nerves. The nerve cells in the olfactory bulb transmit the signals into hippocampus. Because hippocampus is closely related with learning and memory functions, the volatile compounds can be potential drugs in AD therapies.


Assuntos
Doença de Alzheimer/tratamento farmacológico , Óleos Voláteis/uso terapêutico , Compostos Orgânicos Voláteis/uso terapêutico , Animais , Humanos , Óleos Voláteis/química , Compostos Orgânicos Voláteis/química
17.
Chem Biodivers ; 13(2): 151-9, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26880428

RESUMO

The chemical profile of the essential oils in ten populations of the genus Helichrysum Mill. (Asteraceae), collected in the loci classici of the nomenclatural types of the taxa endemic to Sicily, were analyzed. Our results confirm that the analysis of secondary metabolites can be used to fingerprint wild populations of Helichrysum, the chemical profiles being coherent with the systematic arrangement of the investigated populations in three main clusters, referring to the aggregates of H. stoechas, H. rupestre, and H. italicum, all belonging to the section Stoechadina. The correct nomenclatural designation of the investigated populations is discussed and the following two new combinations are proposed: Helichrysum preslianum subsp. compactum (Guss.) Maggio, Bruno, Guarino, Senatore & Ilardi and Helichrysum panormitanum subsp. latifolium Maggio, Bruno, Guarino, Senatore & Ilardi.


Assuntos
Helichrysum/química , Óleos Voláteis/química , Cromatografia Gasosa-Espectrometria de Massas , Helichrysum/classificação , Óleos Voláteis/classificação , Análise de Componente Principal , Sicília
18.
Nat Prod Res ; 30(1): 25-34, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-25880372

RESUMO

The chemical compositions of the essential oil and of the non-polar extracts (petroleum ether, dichloromethane) of the aerial parts (flowers, leaves and stems) of Salvia argentea L. were determined by GC-FID and gas chromatography-mass spectrometry analysis. 14-Hydroxy-α-humulene (40.1%) was recognised as the main constituents of the essential oil of S. argentea, together with 1,3,8-p-menthatriene (12.1%), globulol (7.4%) and ß-sesquiphellandrene (5.8%). Tritriacontane (9.9% and 14.1%), heptacosane (8.4% and 10.5%), hentriacontane (8.3% and 10.9%), tetradecanal (8.4% and 10.2%) and methyldotriacontane (7.9% and 7.6%) were recognised as the main constituents of the extracts in petroleum ether and dichloromethane, respectively, whereas methyl linolenate (36.6% and 13.5%) and methyl myristoleate (10.5% and 18.5%) were recognised as the main constituents of the methylated extracts.


Assuntos
Óleos Voláteis/química , Salvia/química , Ácidos Graxos Monoinsaturados/isolamento & purificação , Flores/química , Cromatografia Gasosa-Espectrometria de Massas , Ácidos Linolênicos/isolamento & purificação , Óleos Voláteis/análise , Parafina/isolamento & purificação , Petróleo , Extratos Vegetais/química , Folhas de Planta/química , Sesquiterpenos/isolamento & purificação , Sicília
19.
Nat Prod Res ; 30(9): 1103-7, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26540480

RESUMO

Although Origanum vulgare (L.) has been deeply analysed at phytochemical level, poor knowledge is available regarding non-volatile compounds such as lipids. The aim of this work was to characterise five wild Sicilian Origanum ecotypes from an agronomic, metabolomic and lipidomic perspective. Serradifalco presented higher dry weight and inflorescences/plant than the others while Favara had a significantly higher number of branches per plant and more extensive flowered stratum. Metabolomic analysis, performed with LC-MS-TOF, allowed a preliminary characterisation of the non-volatile metabolome of the five oregano ecotypes Origanum vulgare ssp. hirtum. Twenty-five metabolites were identified belonging to organic acids, amino acids, lysophosphatidylcholines, carnithines, nucleic bases and lysophosphatidylethanolamines. Lipidomic analysis identified 115 polar plant membrane glycerolipid species. Thirteen of them were differentially present in the two chosen ecotypes. The role of these metabolites in plant physiology from a qualitative and pharmacological point of view was discussed.


Assuntos
Origanum/química , Agricultura , Cromatografia Líquida , Ecótipo , Flores , Lipídeos/química , Espectrometria de Massas , Metabolômica , Origanum/metabolismo , Análise de Componente Principal , Sicília
20.
Chem Biodivers ; 12(5): 781-99, 2015 May.
Artigo em Inglês | MEDLINE | ID: mdl-26010666

RESUMO

The chemical composition of the essential oil isolated from the aerial parts of Pulicaria sicula (L.) Moris was characterized by GC-FID and GC/MS analyses. The oil was particularly rich in oxygenated terpenoids. Among the oxygenated monoterpenes (content of 44.5%), the most abundant were borneol (23.7%), bornyl acetate (6.5%), and isothymol isobutyrate (6.2%). Caryophyllene oxide (10.2%), caryophylladienol I (4.3%), and caryophylla-3,8(13)-dien-5ß-ol (4.4%) were identified as the main constituents among the oxygenated sesquiterpenes. Furthermore, a complete literature review on the composition of the essential oils of all the Pulicaria taxa studied so far was performed and a principal component analysis (PCA) was carried out.


Assuntos
Óleos Voláteis/análise , Óleos Voláteis/química , Componentes Aéreos da Planta/química , Pulicaria/química , Cromatografia Gasosa-Espectrometria de Massas , Estrutura Molecular , Análise de Componente Principal , Sicília
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