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1.
Lik Sprava ; (2): 88-92, 2002.
Artigo em Ucraniano | MEDLINE | ID: mdl-12073272

RESUMO

Data are submitted on individual prophylaxis of damage to the genetical apparatus of bodily normal cells in cytostatic therapy of immunodeficiency states by observing changes in biochemical indices, such as activity of enzymes, content of biologically active substances and hormones.


Assuntos
Biomarcadores/sangue , Doenças do Sistema Imunitário/tratamento farmacológico , Imunossupressores/efeitos adversos , Imunossupressores/uso terapêutico , Criança , Colinesterases/sangue , Humanos , Doenças do Sistema Imunitário/imunologia , Doenças do Sistema Imunitário/fisiopatologia , Mediadores da Inflamação/sangue , Prognóstico , alfa-Amilases/sangue
2.
Lik Sprava ; (1): 88-91, 2002.
Artigo em Russo | MEDLINE | ID: mdl-11944394

RESUMO

Two cases are presented of replication of enteroviruses over a long period of time, that caused a protracted atypical clinical course of infection. No typical clinical markers of infection can be seen in the wake of enterovirus replication; the replication itself can be of a protracted recurrent character. Replication of enteroviruses is capable of inducing a protracted recurrent subfebrile condition in adults that has no clinical signs of immunodeficiency state. Parallel activation of replication of a number of viruses, according to our data, is a widespread phenomenon, which fact is to be taken account of in planning examination and treatment strategies. Laboratory tests are needed for the activation of diseases of enteroviral etiology to be ascertained.


Assuntos
Infecções por Enterovirus/diagnóstico , Infecções por Enterovirus/virologia , Adulto , Infecções por Enterovirus/tratamento farmacológico , Feminino , Humanos , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Tempo
4.
Artigo em Russo | MEDLINE | ID: mdl-8017136

RESUMO

The examination of 99 children immunized with adsorbed DPT vaccine revealed that the intensity of immune response to the diphtheria and tetanus components of the preparation were linked with a number of genetic markers (respectively, HLA-A9, B35, etc.; HLA-A25, B8, etc.) and their combinations. The groups of children with high and low sensitivity include an elevated number of persons with the corresponding titers of antibodies of other specificity. The results obtained in this study should be considered in working out individual immunization schedules.


Assuntos
Anticorpos Antibacterianos/biossíntese , Clostridium tetani/imunologia , Corynebacterium diphtheriae/imunologia , Vacina contra Difteria, Tétano e Coqueluche/imunologia , Adsorção , Anticorpos Antibacterianos/sangue , Especificidade de Anticorpos/genética , Especificidade de Anticorpos/imunologia , Antígenos de Grupos Sanguíneos/genética , Antígenos de Grupos Sanguíneos/imunologia , Marcadores Genéticos/genética , Marcadores Genéticos/imunologia , Antígenos HLA-A/genética , Antígenos HLA-A/imunologia , Antígenos HLA-B/genética , Antígenos HLA-B/imunologia , Humanos , Lactente
5.
Lab Delo ; (6): 34-6, 1991.
Artigo em Russo | MEDLINE | ID: mdl-1717750

RESUMO

A micromodification of the specific leukocyte lysis method was developed; only 0.02 ml of blood is sufficient for the test. The micromodification improved the reproducibility of the technique more than twofold, simplified it, and is recommended for examinations of some populations, including children whose body reactivity is changed and an individual approach to their immunization with TDP vaccine is necessary.


Assuntos
Vacina contra Difteria, Tétano e Coqueluche/efeitos adversos , Hipersensibilidade/imunologia , Leucócitos/imunologia , Pré-Escolar , Vacina contra Difteria, Tétano e Coqueluche/administração & dosagem , Humanos , Técnicas Imunológicas , Técnicas In Vitro , Lactente
6.
Lab Delo ; (2): 20-3, 1990.
Artigo em Russo | MEDLINE | ID: mdl-1692359

RESUMO

The developed procedure for the isolation of mononuclears in density gradient d = 1.0077 g/cm3 consists in separation of the plasma by preliminary centrifugation at 200 g for 7-10 min and isolation of leukocytic mononuclear cells from the cellular sediment diluted with the isotonic solution. The length of centrifugation at 400 g is selected with consideration for the thickness of the density gradient. An anticoagulant is added to the isotonic solution and ficoll-verografin mixture to improve the quality of the cell isolation.


Assuntos
Separação Celular/métodos , Centrifugação com Gradiente de Concentração/métodos , Leucócitos Mononucleares/citologia , Humanos
8.
Artigo em Russo | MEDLINE | ID: mdl-3137742

RESUMO

Blood groups, including rhesus grouping, have been determined in 218 donors and HLA of loci A, B, C in 121 donors immunized with adsorbed tetanus toxoid (40 binding units). The presence of HLA Aw 32, B 8; HLA Bw 16, haplotypes A3 Bw 16 and A1, blank antigens of locus C is linked with the production of, respectively, high or low (less than or equal to 16 I.U./ml) antibody titers. The effectiveness of immunization is influenced by agglutinogen B, which is confirmed by its associations (in a complex with HLA Bw 16 or Bw 35) with low immune response.


Assuntos
Doadores de Sangue , Marcadores Genéticos , Imunização , Toxoide Tetânico/imunologia , Sistema ABO de Grupos Sanguíneos/genética , Sistema ABO de Grupos Sanguíneos/imunologia , Adsorção , Anticorpos Antibacterianos/análise , Clostridium tetani/imunologia , Antígenos HLA/genética , Antígenos HLA/imunologia , Humanos , Sistema do Grupo Sanguíneo Rh-Hr/genética , Sistema do Grupo Sanguíneo Rh-Hr/imunologia , Caracteres Sexuais
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