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1.
Med Sci Monit Basic Res ; 27: e929207, 2021 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-33397841

RESUMO

As of November 25, 2020, over 60 million people have been infected worldwide by COVID-19, causing almost 1.43 million deaths. Puzzling low incidence numbers and milder, non-fatal disease have been observed in Thailand and its Southeast (SE) Asian neighbors. Elusive genetic mechanisms might be operative, as a multitude of genetic factors are widely shared between the SE Asian populations, such as the more than 60 different thalassemia syndromes (principally dominated by the HbE trait). In this study, we have plotted COVID-19 infection and death rates in SE Asian (SEA) countries against heterozygote HbE and thalassemia carrier prevalence. COVID-19 infection and death incidence numbers appear inversely correlated with the prevalence of HbE and thalassemia heterozygote populations. We posit that the evolutionary protective effect of the HbE and other thalassemic variants against malaria and the dengue virus may extend its advantage to resistance to COVID-19 infection, as HbE heterozygote population prevalence appears to be positively correlated with immunity to COVID-19. Host immune system modulations induce antiviral interferon responses and alter structural protein integrity, thereby inhibiting cellular access and viral replication. These changes are possibly engendered by HbE carrier miRNAs. Proving this hypothesis is important, as it may shed light on the mechanism of viral resistance and lead to novel antiviral treatments. This development can thus guide decision-making and action to prevent COVID-19 infection.


Assuntos
/genética , Resistência à Doença/genética , Suscetibilidade a Doenças , Hemoglobina E/genética , Antivirais/uso terapêutico , Grupo com Ancestrais do Continente Asiático , /imunologia , Dengue/genética , Heterozigoto , Humanos , Sistema Imunitário , Interferons , Malária/genética , Pandemias , Prevalência , Tailândia/epidemiologia , Talassemia/epidemiologia , Talassemia/genética
2.
Biomed Res Int ; 2020: 5292090, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32685500

RESUMO

Results: In 221 cycles from 138 patients (104 cycles requiring HLA matching), 90.5% had embryo(s) biopsied for genetic testing. There were 119 embryo transfers for thalassemia (76) and thalassemia-HLA cases (43), respectively, resulting in overall clinical pregnancy rates of 54.6%, implantation rates of 45.7%, and live birth rates of 44.1%. Our dataset included fifteen PGD-HLA live births with successful HSCT in twelve affected siblings, 67% using umbilical cord blood stem cells (UCBSC) as the only SC source. Conclusions: We report favorable thalassemia PGD and PGD-HLA laboratory and clinical outcomes from a single center. The ultimate success in PGD-HLA is of course the cure of a thalassemia-affected sibling by HSCT. Our PGD-HLA HSCT series is the first and largest performed entirely in Asia with twelve successful and two pending cures and predominant UCBSC use.

4.
Fertil Steril ; 91(2): 305-15, 2009 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-19201275

RESUMO

OBJECTIVE: To review currently available literature on the association between imprinting disorders (Beckwith-Wiedemann syndrome [BWS], Angelman syndrome [AS] and retinoblastoma) and assisted reproductive technology (ART) in humans. DESIGN: Publications related to imprinting/epigenetic disorders including BWS, AS, and retinoblastoma with ART, as well as articles publishing outcome of ART, including IVF and ICSI from July 1978 to February 2008, were identified using PubMed, Medline, and EMBASE. RESULT(S): Considerable evidence in animal studies has demonstrated alteration in gene imprinting of embryos cultured in vitro. Publications from Europe, the United States, and Australia have suggested an association between ART and BWS. Importantly, more than 90% of children with BWS that were born after ART had imprinting defects, compared with 40%-50% of children with BWS and conceived without ART. Moreover, there have been other reports suggesting an association between AS and ART. The majority of children with AS born after ART had an imprinting defect as the underlying etiology, specifically loss of methylation of the maternal allele. There was a single report suggesting an increased incidence of retinoblastoma in children conceived with ART. CONCLUSION(S): Because the absolute incidence of imprinting disorders is small (<1:12,000 births), routine screening for imprinting disorders in children conceived by ART is not recommended. Additional large cohort studies of children born after ART are needed to determine whether there is a genuine association between ART and imprinting disorders.


Assuntos
Síndrome de Angelman/genética , Síndrome de Beckwith-Wiedemann/genética , Regulação da Expressão Gênica no Desenvolvimento , Impressão Genômica , Técnicas de Reprodução Assistida/efeitos adversos , Retinoblastoma/genética , Animais , Metilação de DNA , Medicina Baseada em Evidências , Feminino , Humanos , Gravidez , Medição de Risco , Fatores de Risco
5.
Obstet Gynecol ; 112(3): 667-9, 2008 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-18757667

RESUMO

Intrauterine devices (IUDs) are a viable treatment option for immunocompromised women who need contraception or menses suppression. They may also be an alternative treatment for women who have a contraindication to estrogen use. A review of the literature on IUD use in this population is sparse, but currently available data suggest that immunocompromised women are not at greater risk of developing pelvic infections.


Assuntos
Hospedeiro Imunocomprometido , Dispositivos Intrauterinos , Feminino , Humanos
6.
Rev Recent Clin Trials ; 3(2): 104-10, 2008 May.
Artigo em Inglês | MEDLINE | ID: mdl-18474020

RESUMO

In recent years, the success of human oocyte cryopreservation has improved dramatically. Currently, there are a couple hundred babies born worldwide with the use of cryopreserved oocytes. The oocyte survival after freezing/thawing process is in the range of 70-95%, comparable to embryo cryopreservation, depending on techniques used. With the change of cryopreservation media to choline-base, the outcome of oocyte cryopreservation has shown to improve. Moreover, new freezing technique, vitrification, has been developed resulting in better survival outcome without the need of sophisticated equipment. Fertilization rate of cryopreserved oocytes with ICSI is approximately 70-90%, comparable to that of fresh oocytes. However, the pregnancy rate varies from 10-40%. The main reason of varying outcome is the difference in technique of cryopreservation. Clinically, oocyte cryopreservation can be applied to preserve fertility of female cancer survivors, to delay childbearing and to quarantine oocytes in oocyte donor program. Oocyte cryopreservation can also be performed as an alternative to embryo cryopreservation due to ethical consideration.


Assuntos
Criopreservação/métodos , Oócitos , Feminino , Humanos , Técnicas de Reprodução Assistida
7.
J Reprod Med ; 51(6): 493-9, 2006 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-16846090

RESUMO

OBJECTIVE: To assess the embryo formation rate after electrical activation on failed-to-fertilize oocytes after intracytoplasmic sperm injection (ICSI). STUDY DESIGN: A prospective, randomized, experimental study was conducted in our research laboratory at the Reproductive Medicine Unit, Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University, between January and September 2004. One hundred failed-to-fertilize oocytes after ICSI were randomly assigned by stratified allocation according to oocyte grading before ICSI. Fifty unfertilized oocytes were activated with 3 consecutive 40-/microsec pulses of 1.5 kV/cm direct electrical current in buffer solution. The other 50 unfertilized oocytes, composing the control group, were treated in same way but without electrical activation. The embryo formation rates between the groups were compared. RESULTS: Embryo formation rates in the electrically activated group were 80% as compared with 16% in the control group (p < 0.001). CONCLUSION: Failed-to-fertilize oocytes after ICSI seem to be able to resume embryonic development after electrical activation.


Assuntos
Desenvolvimento Embrionário/fisiologia , Fertilização/fisiologia , Oócitos/fisiologia , Injeções de Esperma Intracitoplásmicas , Células Cultivadas , Estimulação Elétrica , Embrião de Mamíferos/anormalidades , Feminino , Humanos , Masculino , Estudos Prospectivos
8.
Int Surg ; 91(5 Suppl): S3-14, 2006.
Artigo em Inglês | MEDLINE | ID: mdl-17436600

RESUMO

The development of techniques to prolong the window of fertility for women has the ability to meet the needs of future populations and their delay in childbearing. With current techniques of cryopreservation of embryos, oocytes, or ovarian tissue, prolongation of fertility with varying success is feasible. In the future, rejuvenation of aging oocytes will offer the opportunity for the aging women to achieve pregnancy with their own genetic offspring. Production of germ cells from embryonic stem cells is another alternative for patient with poor or depleted germ cells. Better understanding of the cause of infertility can be achieved with new techniques to evaluate the oocytes, spermatozoa, and embryos on molecular and genetic levels. With improved understanding of the etiology of infertility, treatment will be more effective. With the advance of new drugs and surgical equipment, treatment of infertility will become more convenient and effective.


Assuntos
Infertilidade Feminina/terapia , Técnicas Reprodutivas/tendências , Animais , Diferenciação Celular , Criopreservação , Fragmentação do DNA , Células-Tronco Embrionárias/fisiologia , Feminino , Humanos , Marcação In Situ das Extremidades Cortadas , Masculino , Robótica , Espermatozoides/fisiologia , Telomerase/metabolismo
9.
Arch Gynecol Obstet ; 268(3): 211-3, 2003 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-12819988

RESUMO

BACKGROUND: Simultaneous or concomitant bilateral adnexal pathology is rare. But simultaneous tubal pregnancy and twisted ovarian cyst is even rarer. CASE: A 25-year-old woman, gravida 2, parity 0, presented with acute abdomen after 12 weeks of amenorrhea. Simultaneous right tubal pregnancy and twisted left ovarian cyst were intraoperatively diagnosed. Right salpingostomy and left salpingo-oophorectomy were performed. The follow-up serum beta-hCG was negative at the nineteenth postoperative day. She was well at discharge and throughout the 4-week follow-up period. CONCLUSION: Although simultaneous or concomitant bilateral adnexal pathology is uncommon, a careful assessment of both adnexa is mandatory especially in the cases with inconsistent site of symptom and pathology in order to avoid undiagnosed simultaneous pathologies that may be missed.


Assuntos
Cistos Ovarianos/diagnóstico , Gravidez Tubária/diagnóstico , Diagnóstico Pré-Natal , Adulto , Diagnóstico Diferencial , Feminino , Humanos , Cistos Ovarianos/complicações , Cistos Ovarianos/patologia , Cistos Ovarianos/cirurgia , Ovariectomia , Gravidez , Primeiro Trimestre da Gravidez , Gravidez Tubária/complicações , Gravidez Tubária/patologia , Gravidez Tubária/cirurgia , Salpingostomia
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