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Infectio ; 23(3): 271-304, July-Sept. 2019. tab
Artigo em Inglês | LILACS-Express | ID: biblio-1002162


Abstract Invasive Candidiasis (IC) and candidemia (as its most frequent manifestation) have become the main cause of opportunistic mycosis at hospital settings. This study, made by members of the Colombian Association of Infectious Diseases (ACIN), was aimed at providing a set of recommendations for the management, follow-up and prevention of IC / candidemia and mucous membrane candida infection in adult, pediatric and neonatal patients in a hospital setting, including the hemato-oncological and critical care units. All the data obtained through an exhaustive search were reviewed and analyzed in a comprehensive manner by all the members of the group, and the recommendations issued are being made after a careful review of the scientific literature available and the consensus of all specialists involved; the emergence of Candida Spp. problem is highlighted and a correct orientation to health professionals regarding the management of patients with candidiasis is provided in a rational and practical way, emphasizing patient evaluation, diagnostic strategies, prophylaxis, empirical treatment, directed treatment and preventative therapy.

Resumen La Candidiasis Invasora (CI) y la candidemia, como su manifestación más frecuente, se ha convertido en la principal causa de micosis oportunista a nivel hospitalario. Este manuscrito realizado por miembros de la Asociación Colombiana de Infectología (ACIN), tuvo como objetivo proporcionar un conjunto de recomendaciones para manejo, seguimiento y prevención de la CI/candidemia y de la infección candidiásica de mucosas, en población adulta, pediátrica y neonatal, en un entorno hospitalario, incluyendo las unidades hemato-oncológicas y unidades de cuidado crítico. Todos los datos obtenidos mediante una búsqueda exhaustiva, fueron revisados y analizados de manera amplia por todos los miembros del grupo, y las recomendaciones emitidas se elaboraron luego de la evaluación de la literatura científica disponible, y el consenso de todos los especialistas involucrados, reconociendo el problema de la emergencia de las infecciones por Candida Spp. y brindando una correcta orientación a los profesionales de la salud sobre el manejo de pacientes con enfermedad candidiásica, de una forma racional y práctica, enfatizando en la evaluación del paciente, estrategias de diagnóstico, profilaxis, tratamiento empírico, tratamiento dirigido y terapia preventiva.

FASEB J ; : fj201900347RR, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31370681


The impact of the mitochondria-targeted antioxidant MitoQ was evaluated in the metabolic alterations and the adipose tissue remodeling associated with obesity. Male Wistar rats were fed either a high-fat diet (HFD; 35% fat) or a standard diet (3.5% fat) for 7 wk and treated with MitoQ (200 µM). A proteomic analysis of visceral adipose tissue from patients with obesity and patients without obesity was performed. MitoQ partially prevented the increase in body weight, adiposity, homeostasis model assessment index, and adipose tissue remodeling in HFD rats. It also ameliorated protein level changes of factors involved in insulin signaling observed in adipose tissue of obese rats: reductions in adiponectin and glucose transporter 4 (GLUT 4) and increases in dipeptidylpeptidase 4, suppressor of cytokine signaling 3 (SOCS3), and insulin receptor substrate 1 phosphorylation. MitoQ prevented down-regulation of adiponectin and GLUT 4 and increases in SOCS3 levels in a TNF-α-induced insulin-resistant 3T3-L1 adipocyte model. MitoQ also ameliorated alterations in mitochondrial proteins observed in obese rats: increases in cyclophylin F and carnitine palmitoyl transferase 1A and reductions in mitofusin1, peroxiredoxin 4, and fumarate hydratase. The proteomic analysis of the visceral adipose tissue from patients with obesity show alterations in mitochondrial proteins similar to those observed in obese rats. Therefore, the data show the beneficial effect of MitoQ in the metabolic dysfunction induced by obesity.-Marín-Royo, G., Rodríguez, C., Le Pape, A., Jurado-López, R., Luaces, M., Antequera, A., Martínez-González, J., Souza-Neto, F. V., Nieto, M. L., Martínez-Martínez, E., Cachofeiro, V. The role of mitochondrial oxidative stress in the metabolic alterations in diet-induced obesity in rats.

Sci Rep ; 9(1): 9607, 2019 Jul 03.
Artigo em Inglês | MEDLINE | ID: mdl-31270370


Although optimal therapy for myocardial infarction includes reperfusion to restore blood flow to the ischemic region, ischemia/reperfusion (IR) also initiates an inflammatory response likely contributing to adverse left ventricular (LV) extracellular matrix (ECM) remodeling. Galectin-3 (Gal-3), a ß-galactoside-binding-lectin, promotes cardiac remodeling and dysfunction. Our aim is to investigate whether Gal-3 pharmacological inhibition using modified citrus pectin (MCP) improves cardiac remodeling and functional changes associated with IR. Wistar rats were treated with MCP from 1 day before until 8 days after IR (coronary artery ligation) injury. Invasive hemodynamics revealed that both LV contractility and LV compliance were impaired in IR rats. LV compliance was improved by MCP treatment 8 days after IR. Cardiac magnetic resonance imaging showed decreased LV perfusion in IR rats, which was improved with MCP. There was no difference in LV hypertrophy in MCP-treated compared to untreated IR rats. However, MCP treatment decreased the ischemic area as well as Gal-3 expression. Gal-3 blockade paralleled lower myocardial inflammation and reduced fibrosis. These novel data showing the benefits of MCP in compliance and ECM remodeling in IR reinforces previously published data showing the therapeutic potential of Gal-3 inhibition.

BMC Complement Altern Med ; 19(1): 153, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31262287


BACKGROUND: Rhus trilobata Nutt. (Anacardiaceae) (RHTR) is a plant of Mexico that is traditionally used as an alternative treatment for several types of cancer. However, the phytochemical composition and potential toxicity of this plant have not been evaluated to support its therapeutic use. Therefore, this study aimed to evaluate the biological activity of RHTR against colorectal adenocarcinoma cells, determine its possible acute toxicity, and analyze its phytochemical composition. METHODS: The traditional preparation was performed by decoction of stems in distilled water (aqueous extract, AE), and flavonoids were concentrated with C18-cartridges and ethyl acetate (flavonoid fraction, FF). The biological activity was evaluated by MTT viability curves and the TUNEL assay in colorectal adenocarcinoma (CACO-2), ovarian epithelium (CHO-K1) and lung/bronchus epithelium (BEAS-2B) cells. The toxicological effect was determined in female BALB/c mice after 24 h and 14 days of intraperitoneal administration of 200 mg/kg AE and FF, respectively. Later, the animals were sacrificed for histopathological observation of organs and sera obtained by retro-orbital bleeding for biochemical marker analysis. Finally, the phytochemical characterization of AE and FF was conducted by UPLC-MSE. RESULTS: In the MTT assays, AE and FF at 5 and 18 µg/mL decreased the viability of CACO-2 cells compared with cells treated with vehicle or normal cells (p ≤ 0.05, ANOVA), with changes in cell morphology and the induction of apoptosis. Anatomical and histological analysis of organs did not reveal important pathological lesions at the time of assessment. Additionally, biochemical markers remained normal and showed no differences from those of the control group after 24 h and 14 days of treatment (p ≤ 0.05, ANOVA). Finally, UPLC-MSE analysis revealed 173 compounds in AE-RHTR, primarily flavonoids, fatty acids and phenolic acids. The most abundant compounds in AE and FF were quercetin and myricetin derivates (glycosides), methyl gallate, epigallocatechin-3-cinnamate, ß-PGG, fisetin and margaric acid, which might be related to the anticancer properties of RHTR. CONCLUSION: RHTR exhibits biological activity against cancer cells and does not present adverse toxicological effects during its in vivo administration, supporting its traditional use.

Int J Infect Dis ; 87: 60-66, 2019 Jul 19.
Artigo em Inglês | MEDLINE | ID: mdl-31330321


OBJECTIVES: Methicillin-resistant Staphylococcus aureus (MRSA) skin and soft tissue infections (SSTIs) represent a major clinical problem in Colombia. The aim of this study was to evaluate the risk factors associated with MRSA SSTI in Colombia. METHODS: A multicenter cohort study with nested case-control design was performed. Patients with an SSTI with at least 48h of inpatient care were included. Patients with an MRSA SSTI were considered the case group and patients with either a non-MRSA SSTI or with an Methicillin-susceptible S. aureus (MSSA) SSTI were the control groups. A multivariate logistic regression approach was used to evaluate risk factors associated with MRSA SSTI with two different statistical models. RESULTS: A total 1134 patients were included. Cultures were positive for 498 patients, of which 52% (n=259) were Staphylococcus aureus. MRSA was confirmed in 68.3% of the S. aureus cultures. In the first model, independent risk factors for MRSA SSTI were identified as the presence of abscess (P<0.0001), cellulitis (P=0.0007), age 18-44 years (P=0.001), and previous outpatient treatment in the previous index visit (P=0.003); surgical site infection was a protective factor (P=0.008). In the second model, the main risk factor found was previous outpatient treatment in the previous index visit (P=0.013). CONCLUSIONS: Community-acquired SSTIs in Colombia are commonly caused by MRSA. Therefore, clinicians should consider MRSA when designing the initial empirical treatment for purulent SSTI in Colombia, although there seems to be low awareness of this fact.

Clin Sci (Lond) ; 133(14): 1537-1548, 2019 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-31285364


Background: Soluble ST2 (interleukin 1 receptor-like 1) (sST2) is involved in inflammatory diseases and increased in heart failure (HF). We herein investigated sST2 effects on oxidative stress and inflammation in human cardiac fibroblasts and its pathological role in human aortic stenosis (AS).Methods and results: Using proteomics and immunodetection approaches, we have identified that sST2 down-regulated mitofusin-1 (MFN-1), a protein involved in mitochondrial fusion, in human cardiac fibroblasts. In parallel, sST2 increased nitrotyrosine, protein oxidation and peroxide production. Moreover, sST2 enhanced the secretion of pro-inflammatory cytokines interleukin (IL)-6, IL-1ß and monocyte chemoattractant protein-1 (CCL-2). Pharmacological inhibition of transcriptional factor nuclear factor κB (NFκB) restored MFN-1 levels and improved oxidative status and inflammation in cardiac fibroblasts. Mito-Tempo, a mitochondria-specific superoxide scavenger, as well as Resveratrol, a general antioxidant, attenuated oxidative stress and inflammation induced by sST2. In myocardial biopsies from 26 AS patients, sST2 up-regulation paralleled a decrease in MFN-1. Cardiac sST2 inversely correlated with MFN-1 levels and positively associated with IL-6 and CCL-2 in myocardial biopsies from AS patients.Conclusions: sST2 affected mitochondrial fusion in human cardiac fibroblasts, increasing oxidative stress production and inflammatory markers secretion. The blockade of NFκB or mitochondrial reactive oxygen species restored MFN-1 expression, improving oxidative stress status and reducing inflammatory markers secretion. In human AS, cardiac sST2 levels associated with oxidative stress and inflammation. The present study reveals a new pathogenic pathway by which sST2 promotes oxidative stress and inflammation contributing to cardiac damage.

Acta colomb. psicol ; 22(1): 107-117, ene.-jun. 2019. tab, graf
Artigo em Espanhol | LILACS | ID: biblio-989077


Resumen La desconexión moral tiene como consecuencia efectos negativos para el desarrollo psicosocial en la niñez, por lo cual se hace necesario contar con escalas para evaluarla. En el presente estudio se analizaron las propiedades psicométricas de la escala Desconexión Moral en Situaciones de Acoso en niños (DMAE). Participaron 661 estudiantes mexicanos de 5.º (51 %) y 6.º grado (49 %), 48 % niñas (M edad = 10.51, DE = .64 años) y 52 % niños (M edad = 10.59, DE = .68 años). Se analizó la validez (estructura interna y concurrente), invarianza de medida para ambos sexos y fiabilidad de la escala. De los resultados del análisis factorial confirmatorio se infirió que el modelo de medición multidimensional que mide la justificación moral, la difusión de la responsabilidad y la atribución de la culpa presenta mejor ajuste a los datos que el unidimensional. Además, se encontró que la DMAE presenta invariancia de medición en ambos sexos y evidencias de validez concurrente. Se concluye que la escala cuenta con propiedades psicométricas adecuadas para la medición de la desconexión moral en niños mexicanos.

Resumo A desconexão moral tem como consequência efeitos negativos para o desenvolvimento psicossocial na infância, o que torna necessário contar com escalas para avaliá-la. Neste estudo, foram analisadas as propriedades psicométricas da escala Desconexão Moral em Situações de Assédio em Crianças. Participaram 661 estudantes mexicanos do 5º (51 %) e 6º anos (49 %), 48 % meninas (M idade = 10.51, DP = .64 anos) e 52 % meninos (M idade = 10.59, DP = .68 anos). Foi analisada a validade (estrutura interna e concorrente), invariância de medida para ambos os sexos e confiabilidade da escala. Dos resultados da análise fatorial confirmatória, foi inferido que o modelo de medição multidimensional que mede a justificativa moral, a difusão da responsabilidade e a atribuição da culpa apresenta melhor ajuste aos dados do que o unidimensional. Além disso, foi constatado que a escala apresenta invariância de medida em ambos os sexos e evidências de validade concorrente. Concluise que a escala possui propriedades psicométricas adequadas para a medição da desconexão moral em crianças mexicanas.

Abstract The moral disengagement has negative effects for psychosocial development in childhood, which makes it necessary to have scales to evaluate it. The present study aimed to analyze the psychometric properties of the Moral Disengagement in Children Situations of Bullying (DMAE, its initials in Spanish) scale. 661 Mexican students participated from 5th grade (51%) and 6th grade (49%), 48% girls (M age = 10.51, SD = .64 years) and 52% boys (M age = 10.59, SD = .68 years). The validity (internal and concurrent), measurement invariance for both sexes and reliability of the scale were analyzed. From the results of the confirmatory factor analysis, it was inferred that the multidimensional measurement model that measures moral justification, diffusion of responsibility and attribution of blame presents a better fit to the data than the one-dimensional one. It was also found that the DMAE presents measurement invariance in both sexes and evidence of concurrent validity. It was concluded that the scale has adequate psychometric properties for the measurement of moral disengagement in Mexican children

Humanos , Masculino , Feminino , Criança , Avaliação Educacional , Emoções , Bullying , Status Moral
Rev Med Inst Mex Seguro Soc ; 57(1): 6, 2019 Apr 01.
Artigo em Espanhol | MEDLINE | ID: mdl-31071247


In this letter to the editor it is discussed the use of Tru-cut needle in pleural biopsy and its profitability for the diagnosis of mesothelioma; it is exposed as well its usefulness, having the appropriate training, to obtain guiding results in obese people.

Hypertension ; 73(3): 602-611, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30612490


Myocardial fibrosis is a main contributor to the development of heart failure (HF). CT-1 (cardiotrophin-1) and Gal-3 (galectin-3) are increased in HF and associated with myocardial fibrosis. The aim of this study is to analyze whether CT-1 regulates Gal-3. Proteomic analysis revealed that Gal-3 was upregulated by CT-1 in human cardiac fibroblasts in parallel with other profibrotic and proinflammatory markers. CT-1 upregulation of Gal-3 was mediated by ERK (extracellular signal-regulated kinase) 1/2 and Stat-3 (signal transducer and activator of transcription 3) pathways. Male Wistar rats and B6CBAF1 mice treated with CT-1 (20 µg/kg per day) presented higher cardiac Gal-3 levels and myocardial fibrosis. In CT-1-treated rats, direct correlations were found between cardiac CT-1 and Gal-3 levels, as well as between Gal-3 and perivascular fibrosis. Gal-3 genetic disruption in human cardiac fibroblasts and pharmacological Gal-3 inhibition in mice prevented the profibrotic and proinflammatory effects of CT-1. Dahl salt-sensitive hypertensive rats with diastolic dysfunction showed increased cardiac CT-1 and Gal-3 expression together with cardiac fibrosis and inflammation. CT-1 and Gal-3 directly correlated with myocardial fibrosis. In HF patients, myocardial and plasma CT-1 and Gal-3 were increased and directly correlated. In addition, HF patients with high CT-1 and Gal-3 plasma levels presented an increased risk of cardiovascular death. Our data suggest that CT-1 upregulates Gal-3 which, in turn, mediates the proinflammatory and profibrotic myocardial effects of CT-1. The elevation of both molecules in HF patients identifies a subgroup of patients with a higher risk of cardiovascular mortality. The CT-1/Gal-3 axis emerges as a candidate therapeutic target and a potential prognostic biomarker in HF.

Sci Rep ; 9(1): 444, 2019 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-30679580


Cardiac lipotoxicity is involved in the cardiac functional consequences associated with obesity. Therefore, the aim of this study was to explore whether changes in the mitochondrial lipid cardiac profile could reflect differences in cardiac function and structure in obese and non-obese rats with myocardial infarction (MI). Whether these changes can also be reflected in a specific plasma miRNA signature as markers of cardiac damage was also evaluated. Rats were fed with either standard (3.5% fat) or high fat diet (35% fat) for 6 weeks before the induction of MI and sacrificed 4 weeks later. MI showed cardiac lipotoxicity independently of the presence of obesity, although obese and non-obese rats did not present the same cardiac lipid profile at mitochondrial level. Several cardiac lipid species in mitochondria, including cardiolipins and triglycerides, were associated with myocardial fibrosis, with mitochondrial triglyceride levels being independently associated with it; this supports that lipotoxicity can affect cardiac function. MI down-regulated plasma levels of miRNA 15b-5p and 194-5p in obese and non-obese animals, which were associated with cardiac function, mitochondrial lipids and myocardial fibrosis, with miRNA 15b-5p levels being independently associated with cardiac fibrosis. This could support that lipotoxicity could affect heart function by modulating plasma miRNAs.

Angew Chem Int Ed Engl ; 58(11): 3382-3386, 2019 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-30620428


The reactivity of the As-zincosilaarsene LZn-As=SiL' A (L=[CH(CMeNDipp)2 ]- , Dipp=2,6-i Pr2 C6 H3 , L'=[{C(H)N(2,6-i Pr2 -C6 H3 )}2 ]2- ) towards small molecules was investigated. Due to the pronounced zwitterionic character of the Si=As bond of A, it undergoes addition reactions with H2 O and NH3 , forming LZnAs(H)SiOH(L') 1 and LZnAs(H)SiNH2 (L') 2. Oxygenation of A with N2 O at -60 °C furnishes the deep blue 1,2-disiloxydiarsene, [LZnOSi(L')As]2 4, presumably via dimerization of the arsinidene intermediate LZnOSi(L')As 3. Oxygenation of A with CO2 leads to the monomeric arsaethynolato siloxido zinc complex LZnOSi(L')(OC≡As) 5, essentially trapping the intermediary arsinidene 3 with liberated CO following initial oxidation of the Si=As bond. DFT calculations confirm the ambident coordination mode of the anionic [AsCO] ligand in solution, with the O-arsaethynolato [As≡C-O].- in 5, and the As-arsaketenylido ligand mode [O=C=As]- present in LZnO-Si(L')(-As=C=O) 5' akin to the analogous phosphorus system, [PCO]- .

Rev. colomb. psicol ; 27(2): 177-192, jul.-dic. 2018. tab, graf
Artigo em Espanhol | LILACS-Express | ID: biblio-978311


Resumen El estudio se propuso analizar las propiedades psicométricas del Participant Role Appoach (PRA) para medir roles de espectadores en el acoso escolar en pre-adolescentes. Participaron 787 estudiantes, 415 (52.7%) niños (M edad=10.59, DE=.66 años) y 372 (47.3%) niñas (M edad=10.51, DE=.64 años). Los resultados del análisis factorial confirmatorio sugirieron que el modelo de tres roles (pro-acoso, pro-social y no comprometido) se ajusta mejor a los datos que el de cuatro roles (alentador, asistente, defensor y no comprometido). La PRA mostró invariancia de medición en ambos sexos, y evidencias de validez concurrente. Se concluyó que la escala posee propiedades psicométricas adecuadas para la medición de roles de espectadores en pre-adolescentes.

Abstract The objective of this research was to analyze the psychometric properties of the Positive Adolescent Development Value Scale (EVDPA, according to its acronym in Spanish) in Chilean students. The sample for the study was made up of 2250 adolescent male and female students between the ages of 12 and 21, from 25 secondary schools in Chile. A robust analysis procedure based on the cross-validation method was used. The results of the exploratory and confirmatory factor analysis made it possible to obtain a reduced factorial structure with three latent factors: social values, personal values, and individualistic values. Evidence of reliability due to internal coherence was favorable. The conclusion is that despite the significant reduction of items and first-order dimensions, the EVDPA delivers sufficient evidence and can be used in the population of Chilean adolescent students.

Resumo O estudo propôs-se a analisar as propriedades psicométricas do Participant Role Approach (PRA) para medir os papéis de observadores no bullying em pré-adolescentes. Participaram 787 estudantes, 415 (52.7%) meninos (M idade=io.59, DP=.66 anos) e 372 (47.3%) meninas (M idade=io.5i, DP=.64 anos). Os resultados da análise fatorial confirmatoria sugeriram que o modelo de três papéis (pró-bullying, pró-social e não comprometido) ajusta-se melhor aos dados do que o modelo de quatro papéis (encorajador, assistente, defensor e não comprometido). A PRA mostrou invariância de medição em ambos os sexos e evidências de validez concorrente. Concluiu-se que a escala tem propriedades psicométricas adequadas para a medição de papéis de observadores em pré-adolescentes.

Rev. medica electron ; 40(3): 806-814, may.-jun. 2018. ilus
Artigo em Espanhol | LILACS-Express | ID: biblio-961252


RESUMEN La extrofia vesical es una grave anomalía del desarrollo embriológico del seno urogenital y del sistema esquelético vecino. Se caracteriza por una falla de la pared anterior del abdomen y de la vejiga, cuya cara posterior-inferior, evertida hacia afuera, protruye en forma de hernia y exterioriza por completo el trígono, con los meatos ureterales eyaculando directamente hacia la pared abdominal; la asociación con epispadias es la regla. Se describió el caso de una paciente pediátrica con diagnóstico del complejo extrofia vesical-epispadias, entidad poco frecuente en nuestro medio, la cual evolucionó de manera favorable y se encuentra en espera de un segundo tiempo quirúrgico como parte de su tratamiento.

ABSTRACT The bladder exstrophy is a serious anomaly of theurogenital sinus´ embryologic development and the close skeletal system, characterized by a failure of the abdomen anterior wall and bladder, whose posterior inferior face, turned outside out, protrudes like an hernia and completely exteriorizes the trigone, with the ureteral meatuses ejaculating right down to the abdominal wall; the association with epispadias is the rule. It was described the case of a pediatric patient with diagnosis of exstrophy- epispadias complex, infrequent entity in our country, who has evolved with great results and is waiting a second surgical time to finish her treatment.

Sci Rep ; 8(1): 6801, 2018 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-29717226


Aldosterone (Aldo) contributes to mitochondrial dysfunction and cardiac oxidative stress. Using a proteomic approach, A-kinase anchor protein (AKAP)-12 has been identified as a down-regulated protein by Aldo in human cardiac fibroblasts. We aim to characterize whether AKAP-12 down-regulation could be a deleterious mechanism which induces mitochondrial dysfunction and oxidative stress in cardiac cells. Aldo down-regulated AKAP-12 via its mineralocorticoid receptor, increased oxidative stress and induced mitochondrial dysfunction characterized by decreased mitochondrial-DNA and Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) expressions in human cardiac fibroblasts. CRISPR/Cas9-mediated knock-down of AKAP-12 produced similar deleterious effects in human cardiac fibroblasts. CRISPR/Cas9-mediated activation of AKAP-12 blunted Aldo effects on mitochondrial dysfunction and oxidative stress in human cardiac fibroblasts. In Aldo-salt-treated rats, cardiac AKAP-12, mitochondrial-DNA and PGC-1α expressions were decreased and paralleled increased oxidative stress. In myocardial biopsies from patients with aortic stenosis (AS, n = 26), AKAP-12, mitochondrial-DNA and PGC-1α expressions were decreased as compared to Controls (n = 13). Circulating Aldo levels inversely correlated with cardiac AKAP-12. PGC-1α positively associated with AKAP-12 and with mitochondrial-DNA. Aldo decreased AKAP-12 expression, impairing mitochondrial biogenesis and increasing cardiac oxidative stress. AKAP-12 down-regulation triggered by Aldo may represent an important event in the development of mitochondrial dysfunction and cardiac oxidative stress.

Clin Sci (Lond) ; 132(9): 909-923, 2018 May 16.
Artigo em Inglês | MEDLINE | ID: mdl-29739822


Neutrophil gelatinase-associated lipocalin (NGAL) is a small circulating protein that is highly modulated in a wide variety of pathological situations, making it a useful biomarker of various disease states. It is one of the best markers of acute kidney injury, as it is rapidly released after tubular damage. However, a growing body of evidence highlights an important role for NGAL beyond that of a biomarker of renal dysfunction. Indeed, numerous studies have demonstrated a role for NGAL in both cardiovascular and renal diseases. In the present review, we summarize current knowledge concerning the involvement of NGAL in cardiovascular and renal diseases and discuss the various mechanisms underlying its pathological implications.

Clin Sci (Lond) ; 132(13): 1471-1485, 2018 Jul 18.
Artigo em Inglês | MEDLINE | ID: mdl-29674526


Galectin-3 (Gal-3) is increased in heart failure (HF) and promotes cardiac fibrosis and inflammation. We investigated whether Gal-3 modulates oxidative stress in human cardiac fibroblasts, in experimental animal models and in human aortic stenosis (AS). Using proteomics and immunodetection approaches, we have identified that Gal-3 down-regulated the antioxidant peroxiredoxin-4 (Prx-4) in cardiac fibroblasts. In parallel, Gal-3 increased peroxide, nitrotyrosine, malondialdehyde, and N-carboxymethyl-lysine levels and decreased total antioxidant capacity. Gal-3 decreased prohibitin-2 expression without modifying other mitochondrial proteins. Prx-4 silencing increased oxidative stress markers. In Gal-3-silenced cells and in heart from Gal-3 knockout mice, Prx-4 was increased and oxidative stress markers were decreased. Pharmacological inhibition of Gal-3 with modified citrus pectin restored cardiac Prx-4 as well as prohibitin-2 levels and improved oxidative status in spontaneously hypertensive rats. In serum from 87 patients with AS, Gal-3 negatively correlated with total antioxidant capacity and positively correlated with peroxide. In myocardial biopsies from 26 AS patients, Gal-3 up-regulation paralleled a decrease in Prx-4 and in prohibitin-2. Cardiac Gal-3 inversely correlated with Prx-4 levels in myocardial biopsies. These data suggest that Gal-3 decreased Prx-4 antioxidant system in cardiac fibroblasts, increasing oxidative stress. In pathological models presenting enhanced cardiac Gal-3, the decrease in Prx-4 expression paralleled increased oxidative stress. Gal-3 blockade restored Prx-4 expression and improved oxidative stress status. In AS, circulating levels of Gal-3 could reflect oxidative stress. The alteration of the balance between antioxidant systems and reactive oxygen species production could be a new pathogenic mechanism by which Gal-3 induces cardiac damage in HF.

Chem Commun (Camb) ; 54(48): 6124-6127, 2018 Jun 12.
Artigo em Inglês | MEDLINE | ID: mdl-29682662


The decarbonylation of the first zinco(ii) arsaketene complexes LZnAsCO (2) and LZn(AsCO)(NHC) (4) (L = {CH(CMeNDipp)2}-, Dipp = 2,6-iPr2C6H3; NHC = [C(Me)N(iPr)]2C:) has been investigated in the presence of the N-heterocyclic silylenes, tBuNHSi (tBuNHSi = [C(H)N(tBu)]2Si:) and DippNHSi (DippNHSi = [C(H)N(2,6-iPr2-C6H3)]2Si:). Depending on the steric demand of the NHSi donor, dimers or monomers of silylene-stabilised arsinidenes are isolated. The bonding situations in all of the novel arsinidene complexes have been elucidated through X-ray diffraction analyses and theoretical calculations.

Rev Chilena Infectol ; 35(1): 83-87, 2018.
Artigo em Espanhol | MEDLINE | ID: mdl-29652977


Sporotrichosis is the most common subcutaneous or implantation mycosis in Mexico. The case of a preauricular cutaneous-fixed sporotrichosis simulating atypical bacterial cellulitis is reported in an elderly patient with no history of trauma. The biopsy showed a suppurative granuloma with scarce yeast. Sporothrix schenckii was identified in the culture and confirmed by molecular biology. She was treated with itraconazole and a clinical and mycological cure was obtained. The case of atypical presentation is presented, coming from a semi-arid zone with extreme weather.

Celulite (Flegmão)/microbiologia , Celulite (Flegmão)/patologia , Otopatias/microbiologia , Otopatias/patologia , Esporotricose/patologia , Idoso de 80 Anos ou mais , Antifúngicos/uso terapêutico , Biópsia , Diagnóstico Diferencial , Otopatias/tratamento farmacológico , Feminino , Humanos , Itraconazol/uso terapêutico , Sporothrix/isolamento & purificação , Esporotricose/tratamento farmacológico , Resultado do Tratamento
Int J Cardiol ; 258: 217-223, 2018 05 01.
Artigo em Inglês | MEDLINE | ID: mdl-29544935


AIMS: Galectin-3 (Gal-3), a ß-galactoside-binding lectin involved in cardiac inflammation and fibrosis, could regulate oxidative stress, although the mechanisms have not been elucidated. We herein investigated the changes in oxidative stress-related mediators induced by Gal-3 in human cardiac fibroblasts and in pathological animal and human models of cardiac diseases. RESULTS: Using quantitative proteomics and immunodetection approaches, we have identified that Gal-3 down-regulated fumarate hydratase (FH) in human cardiac fibroblasts. In parallel, Gal-3 increased fumarate production in a time-dependent manner. Gal-3 treatment enhanced carbonylated proteins detected through OxyBlot technique. Interestingly, treatment of cells with fumarate induced oxidative stress, enhanced fibroblast activation markers and increased collagen and interleukin-6 secretion. In Gal-3-silenced cells and in heart from Gal-3 knock-out mice, FH was increased and fumarate was decreased. In myocardial biopsies from patients with aortic stenosis (AS, n=26), FH levels were decreased as compared to Controls (n=13). Cardiac Gal-3 inversely correlated with FH levels in myocardial biopsies. In an experimental model of AS rats, pharmacological inhibition of Gal-3 restored cardiac FH, decreased fumarate concentration and improved oxidative status. CONCLUSION: In human cardiac fibroblasts, Gal-3 decreased FH expression increasing fumarate concentration and promoting oxidative stress. In human AS, cardiac levels of Gal-3 inversely associated with FH. Gal-3 blockade restored FH and improved fumarate and oxidative stress status in AS rats. FH is therefore a key molecule mediating Gal-3-induced oxidative stress in cardiac cells.

Fibroblastos/metabolismo , Fumarato Hidratase/fisiologia , Galectina 3/metabolismo , Miocárdio/metabolismo , Estresse Oxidativo/fisiologia , Animais , Células Cultivadas , Fibroblastos/patologia , Galectina 3/deficiência , Humanos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Miocárdio/patologia , Ratos , Ratos Wistar
Actas Esp Psiquiatr ; 46(1): 1-11, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29417976


OBJECTIVE: To describe the relationship between neuropsychological variables and serum cortisol levels as a measure of physiological stress in patients with fibromyalgia. METHODOLOGY: A sample of 60 women was intentionally selected: 30 with Fibromyalgia diagnosis and 30 with normal controls. Cortisol levels were determined using two blood samples (AM and PM) and a neuropsychological and emotional battery was applied with a standardized protocol in Colombian population to evaluate different cognitive domains. Comparative and correlational non-parametric analyzes were performed, a multiple regression analysis to determine influences between variables. RESULTS: Significant differences between the study groups in the neuropsychological variables (attention, memory, language, visual-constructive praxis and executive functions (EF), (p<0.05) were found, obtaining better scores in the control group. Significant correlations between the cortisol profile, with false acknowledgments of Rey auditory- verbal learning test, and with perseverative errors of the Wisconsin test were found. Multiple regression analysis predicts the influence of memory and EF variables on the cortisol profile in an 88.7%. CONCLUSIONS: The findings show that, in patients with FM, there are neuropsychological alterations, mainly in executive functioning (cognitive flexibility) and episodic memory (evocation and storage). Likewise, executive dysfunction is related to physiological stress reciprocally and in turn are conditioned by emotional alterations such as symptoms of depression, which supports the neurophysiological model that compromises the hypothalamic-pituitary-adrenal axis and the prefrontal cortex, rich in corticosteroid receptors.