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3.
Stud Health Technol Inform ; 264: 1720-1721, 2019 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-31438310

RESUMO

This study was designed to develop a wearable learning support system that enables novices to learn the skill for blood withdrawal while imitating its images displayed in front of them. For them to master "tacit knowledge," such as "proficient art" and "knacks," in intravenous injection and blood drawing techniques. This paper outlines a learning support system incorporating augmented reality (AR), which we have developed based on earlier studies.


Assuntos
Conhecimento , Aprendizagem , Enfermagem , Competência Clínica , Enfermagem/normas
4.
No Shinkei Geka ; 47(4): 449-454, 2019 Apr.
Artigo em Japonês | MEDLINE | ID: mdl-31061230

RESUMO

A female neonate presented with a cranial mass in the left parietal lobe region. She was born through Cesarean section, and there was no history of birth trauma or head injury. Her mother noticed the mass two weeks after birth. It increased slowly in size and turned stiff. CT showed a skull tumor with calcification. MRI findings, with no vascular abnormalities, indicated a possibility of cephalohematoma. 67Ga scintigraphy showed no abnormal uptake in the cranial mass or any other systemic lesion. We performed an operation to examine the histology and to adjust the shape of the skull. The diagnosis from the histological examination was cephalohematoma with calcification. Cephalohematoma occurs less commonly in the case of Cesarean section and usually resolves spontaneously without any intervention. Some studies have reported birth trauma even though the baby was born through a scheduled Cesarean section. Even if there is no definite history of head trauma, it is necessary to consider cephalohematoma when we examine a cranial mass of a neonate.


Assuntos
Encefalopatias , Cesárea , Hematoma , Neoplasias Cranianas , Encefalopatias/diagnóstico por imagem , Encefalopatias/cirurgia , Feminino , Hematoma/diagnóstico por imagem , Hematoma/cirurgia , Humanos , Recém-Nascido , Imagem por Ressonância Magnética , Gravidez , Crânio , Neoplasias Cranianas/diagnóstico por imagem , Neoplasias Cranianas/cirurgia
5.
Nutrients ; 10(11)2018 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-30463354

RESUMO

A higher protein intake is recommended for athletes compared to healthy non-exercising individuals. Additionally, the distribution and quality (i.e., leucine content) of the proteins consumed throughout the day should be optimized. This study aimed to determine the nitrogen balance and distribution of protein and amino acid intakes in competitive swimmers during the general preparation phase. Thirteen swimmers (age: 19.7 ± 1.0 years; VO2max: 63.9 ± 3.7 mL·kg-1·min-1, mean ± standard deviation) participated in a five-day experimental training period. Nutrient intakes were assessed using dietary records. Nitrogen balance was calculated from the daily protein intake and urinary nitrogen excretion. The intake amounts of amino acids and protein at seven eating occasions were determined. The average and population-safe intakes for zero nitrogen balance were estimated at 1.43 and 1.92 g·kg-1·day-1, respectively. The intake amounts of protein and leucine at breakfast, lunch, and dinner satisfied current guidelines for the maximization of muscle protein synthesis, but not in the other four occasions. The population-safe protein intake level in competitive swimmers was in the upper range (i.e., 1.2⁻2.0 g·kg-1·day-1) of the current recommendations for athletes. The protein intake distribution and quality throughout the day may be suboptimal for the maximization of the skeletal muscle adaptive response to training.


Assuntos
Aminoácidos/administração & dosagem , Atletas , Nitrogênio/metabolismo , Natação/fisiologia , Adolescente , Dieta , Proteínas na Dieta/administração & dosagem , Ingestão de Energia , Metabolismo Energético , Humanos , Leucina/administração & dosagem , Masculino , Refeições , Músculo Esquelético/fisiologia , Nitrogênio/urina , Política Nutricional , Necessidades Nutricionais , Resistência Física/fisiologia , Adulto Jovem
6.
Nat Commun ; 9(1): 3843, 2018 09 21.
Artigo em Inglês | MEDLINE | ID: mdl-30242156

RESUMO

How genetic information is modified to generate phenotypic variation within a species is one of the central questions in evolutionary biology. Here we focus on the striking intraspecific diversity of >200 aposematic elytral (forewing) colour patterns of the multicoloured Asian ladybird beetle, Harmonia axyridis, which is regulated by a tightly linked genetic locus h. Our loss-of-function analyses, genetic association studies, de novo genome assemblies, and gene expression data reveal that the GATA transcription factor gene pannier is the major regulatory gene located at the h locus, and suggest that repeated inversions and cis-regulatory modifications at pannier led to the expansion of colour pattern variation in H. axyridis. Moreover, we show that the colour-patterning function of pannier is conserved in the seven-spotted ladybird beetle, Coccinella septempunctata, suggesting that H. axyridis' extraordinary intraspecific variation may have arisen from ancient modifications in conserved elytral colour-patterning mechanisms in ladybird beetles.


Assuntos
Besouros/genética , Evolução Molecular , Fatores de Transcrição GATA/genética , Pigmentação/genética , Animais , Carotenoides/metabolismo , Besouros/crescimento & desenvolvimento , Besouros/metabolismo , Cor , Feminino , Fatores de Transcrição GATA/metabolismo , Genoma de Inseto , Proteínas de Insetos/genética , Proteínas de Insetos/metabolismo , Íntrons , Masculino , Melaninas/metabolismo , Polimorfismo Genético , Pupa/crescimento & desenvolvimento
7.
Waste Manag ; 71: 2-9, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29102448

RESUMO

In this study, the amount of prevented household waste in Kyoto city was quantified using three methods. Subsequently, the greenhouse gas (GHG) emission reduction by waste prevention was calculated in order to monitor the impact of waste prevention. The methods of quantification were "relative change from baseline year (a)," "absolute change from potential waste generation (b)," and "absolute amount of activities (c)." Method (a) was popular for measuring waste prevention, but method (b) was the original approach to determine the absolute amount of waste prevention by estimating the potential waste generation. Method (c) also provided the absolute value utilizing the information of activities. Methods (b) and (c) enable the evaluation of the waste prevention activities with a similar baseline for recycling. Methods (b) and (c) gave significantly higher GHG reductions than method (a) because of the difference in baseline between them. Therefore, setting a baseline is very important for evaluating waste prevention. In practice, when focusing on the monitoring of a specific policy or campaign, method (a) is an appropriate option. On the other hand, when comparing the total impact of waste prevention to that of recycling, methods (b) and (c) should be applied.


Assuntos
Utensílios Domésticos , Reciclagem , Eliminação de Resíduos , Cidades , Monitoramento Ambiental , Políticas , Gerenciamento de Resíduos
8.
Adv Sci (Weinh) ; 4(10): 1700176, 2017 10.
Artigo em Inglês | MEDLINE | ID: mdl-29051858

RESUMO

The oxygen evolution reaction (OER) plays a key role in emerging energy conversion technologies such as rechargeable metal-air batteries, and direct solar water splitting. Herein, a remarkably low overpotential of ≈150 mV at 10 mA cm-2disk in alkaline solutions using one of the non-Fermi liquids, Hg2Ru2O7, is reported. Hg2Ru2O7 displays a rapid increase in current density and excellent durability as an OER catalyst. This outstanding catalytic performance is realized through the coexistence of localized d-bands with the metallic state that is unique to non-Fermi liquids. The findings indicate that non-Fermi liquids could greatly improve the design of highly active OER catalysts.

9.
J Alzheimers Dis ; 56(1): 1-23, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-27911310

RESUMO

We previously reported that neuroinflammation contributes to the amnesia of AßPPswe/PSEN1dE9 Alzheimer's disease model mice fed a high-fat diet to induce type-2 diabetes (T2DM-AD mice), but the underlying mechanism for the memory decline remained unclear. Recent studies have suggested that cholinergic modulation is involved in neuroinflammatory cellular reactions including neurogenesis and gliosis, and in memory improvement. In this study, we administered a broad-spectrum cholinesterase inhibitor, rivastigmine (2 mg/kg/day, s.c.), into T2DM-AD mice for 6 weeks, and evaluated their memory performance, neurogenesis, and neuroinflammatory reactions. By two hippocampal-dependent memory tests, the Morris water maze and contextual fear conditioning, rivastigmine improved the memory deterioration of the T2DM-AD mice (n = 8, p < 0.01). The number of newborn neurons in the hippocampal dentate gyrus was 1138±324 (Ave±SEM) in wild-type littermates, 2573±442 in T2DM-AD-Vehicle, and 2165±300 in T2DM-AD-Rivastigmine mice, indicating that neurogenesis was accelerated in the two T2DM-AD groups (n = 5, p < 0.05). The dendritic maturation of new neurons in T2DM-AD-Vehicle mice was severely abrogated, and rivastigmine treatment reversed this retarded maturation. In addition, the hippocampus of T2DM-AD-Vehicle mice showed increased proinflammatory cytokines IL-1ß and TNF-α and gliosis, and rivastigmine treatment blocked these inflammatory reactions. Rivastigmine did not change the insulin abnormality or amyloid pathology in these mice. Thus, cholinergic modulation by rivastigmine treatment led to enhanced neurogenesis and the suppression of gliosis, which together ameliorated the memory decline in T2DM-AD model mice.


Assuntos
Doença de Alzheimer/complicações , Diabetes Mellitus Tipo 2/complicações , Gliose/tratamento farmacológico , Transtornos da Memória , Neurogênese/efeitos dos fármacos , Rivastigmina/farmacologia , Rivastigmina/uso terapêutico , Doença de Alzheimer/genética , Precursor de Proteína beta-Amiloide/genética , Animais , Peso Corporal/efeitos dos fármacos , Peso Corporal/genética , Células Cultivadas , Inibidores da Colinesterase/farmacologia , Inibidores da Colinesterase/uso terapêutico , /fisiologia , Diabetes Mellitus Tipo 2/etiologia , Dieta Hiperlipídica/efeitos adversos , Modelos Animais de Doenças , Gliose/etiologia , Humanos , Insulina/sangue , Aprendizagem em Labirinto/efeitos dos fármacos , Transtornos da Memória/tratamento farmacológico , Transtornos da Memória/etiologia , Transtornos da Memória/genética , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Neurônios/efeitos dos fármacos , Neurônios/metabolismo , Presenilina-1/genética
10.
Int J Cardiovasc Imaging ; 31 Suppl 2: 177-85, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26202159

RESUMO

We evaluated the image quality and diagnostic performance of late iodine enhancement (LIE) in dual-source computed tomography (DSCT) with low kilo-voltage peak (kVp) images and a denoise filter for the detection of acute myocardial infarction (AMI) in comparison with late gadolinium enhancement (LGE) magnetic resonance imaging (MRI). The Hospital Ethics Committee approved the study protocol. Before discharge, 19 patients who received percutaneous coronary intervention after AMI underwent DSCT and 1.5 T MRI. Immediately after coronary computed tomography (CT) angiography, contrast medium was administered at a slow injection rate. LIE-CT scans were acquired via dual-energy CT and reconstructed as 100-, 140-kVp, and mixed images. An iterative three-dimensional edge-preserved smoothing filter was applied to the 100-kVp images to obtain denoised 100-kVp images. The mixed, 140-kVp, 100-kVp, and denoised 100-kVp images were assessed using contrast-to-noise ratio (CNR), and their diagnostic performance in comparison with MRI and infarcted volumes were evaluated. Three hundred four segments of 19 patients were evaluated. Fifty-three segments showed LGE in MRI. The median CNR of the mixed, 140-, 100-kVp and denoised 100-kVp images was 3.49, 1.21, 3.57, and 6.08, respectively. The median CNR was significantly higher in the denoised 100-kVp images than in the other three images (P < 0.05). The denoised 100-kVp images showed the highest diagnostic accuracy and sensitivity. The percentage of myocardium in the four CT image types was significantly correlated with the respective MRI findings. The use of a denoise filter with a low-kVp image can improve CNR, sensitivity, and accuracy in LIE-CT.


Assuntos
Meios de Contraste/administração & dosagem , Gadolínio DTPA/administração & dosagem , Iopamidol/administração & dosagem , Imagem por Ressonância Magnética , Infarto do Miocárdio/diagnóstico por imagem , Infarto do Miocárdio/patologia , Miocárdio/patologia , Interpretação de Imagem Radiográfica Assistida por Computador/métodos , Tomografia Computadorizada por Raios X/métodos , Idoso , Idoso de 80 Anos ou mais , Desenho de Equipamento , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Valor Preditivo dos Testes , Estudos Prospectivos , Reprodutibilidade dos Testes , Sobrevivência de Tecidos
11.
Acta Chim Slov ; 61(3): 453-6, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25286200

RESUMO

Using radio frequency - magnetron sputtering, calcium-doped barium zirconate titanate ((Ba(0.85)Ca(0.15))(Zr(0.1)Ti(0.9))O(3), BCZT) thin films were deposited on Si wafers with different bottom electrodes. The obtained BCZT thin film on a lanthanum nickel oxide (LNO) electrode had a highly c-axis preferred orientation, while the BCZT thin film on a Pt bottom electrode had (111) preferred orientation. Furthermore, the out-of-plane lattice constant of the BCZT on LNO/Si was 3.4% larger than that of the reported bulk material because of the compressive thermal stress from LNO with a large thermal expansion coefficient. This compressive thermal stress engenders an increase of the Curie temperature. The local piezoelectric response of the BCZT thin film on a LNO/Si structure was measured by piezoresponse force microscope.

12.
Springerplus ; 3: 75, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24570849

RESUMO

PURPOSE: The purpose of this study was to investigate the utility incremental diagnostic value of combined assessment with coronary CT angiography (CCTA) and myocardial CT perfusion imaging (CTP) using dual-energy technology with an Adenosine Triphosphate (ATP) load technique. MATERIALS AND METHODS: Twenty-one patients underwent ATP-provocation dual-energy CT and CAG. We compared the diagnostic accuracy with CAG, for ischemic region due coronary stenosis by CCTA alone and CCTA combined with CTP (Combined CCTA/CTP). RESULTS: All of 21 patients CTP images could be evaluated, however 8 CCTA images could not be evaluated by calcification and motion artifact, so assessability was 61.9% (13/21) for CCTA alone, and 100% for Combined CCTA/CTP. With CAG results as a comparison, the sensitivity, specificity, positive predictive value, and negative predictive value were, respectively, 83.3% (20/24), 74.4% (29/39), 66.7% (20/30), and 87.8% (29/33) for CCTA alone, and 66.7% (16/24), 92.3% (36/39), 84.2% (16/19), and 81.8% (36/44) for combined CCTA/CTP. The diagnostic accuracy of the two methods were 77.8% (49/63) and 82.5% (52/63). CONCLUSION: Dual-energy CT may be a useful modality for perfusion assessment and correlated well with the severity of stenosis on CAG. This technique may even be of use in cases of severe calcification in the coronary artery wall.

13.
Int J Pediatr Otorhinolaryngol ; 78(3): 554-7, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-24480121

RESUMO

We report VEMP results in a patient with aplasia of bilateral semicircular canals and a small vestibular cavity. The patient was a 27-year-old male. The computed tomograph showed absolutely no formation of his semicircular canals, together with hypoplasia of his vestibular cavity and cochlea in both ears. His oVEMP was recorded near the extraocular muscles on the left side when elicited by BCV in the Fz. The clinical profile of this patient suggested that oVEMP elicited by BCV recorded near the extraocular muscles originated from otolithic end organs, and not from semicircular canal afferents.


Assuntos
Condução Óssea/fisiologia , Síndrome CHARGE/diagnóstico , Canais Semicirculares/anormalidades , Potenciais Evocados Miogênicos Vestibulares/fisiologia , Adulto , Humanos , Masculino , Doenças Raras , Índice de Gravidade de Doença , Testes de Função Vestibular , Vibração
14.
Neurosci Lett ; 409(2): 118-23, 2006 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-17049741

RESUMO

We recently reported that hypoxic stress induces the expression of HIF-1alpha, HIF-2alpha, cyclooxygenases-2 (COX-2) and VEGF in vivo. In this study, we investigated whether HIF-1alpha, HIF-2alpha, or COX-2 naked DNA induced angiogenesis in a cerebral ischemic model in vivo. We utilized a rat encephalo-myo-synangiosis (EMS) model and inoculated naked DNA into the brain surface. We analyzed whether DNA induced angiogenic factors and neovascularization. New blood vessel formation was detected by anti-Factor VIII staining. A histological section treated with HIF-2alpha or COX-2 DNA showed an increased expression of VEGF with angiogenesis, in comparison to the control DNA. The HIF-1alpha, HIF-2alpha, and COX-2 are able to promote significant angiogenesis development. These results suggest the feasibility of a novel approach for therapeutic angiogenesis of cerebral ischemia in which neovascularization may be indirectly achieved using a transcriptional and cytokine's regulatory strategy.


Assuntos
Fatores de Transcrição Hélice-Alça-Hélice Básicos/genética , Ciclo-Oxigenase 2/genética , DNA/administração & dosagem , DNA/genética , Subunidade alfa do Fator 1 Induzível por Hipóxia/genética , Neovascularização Fisiológica/genética , Animais , Isquemia Encefálica/patologia , Isquemia Encefálica/terapia , Fator VIII , Imuno-Histoquímica , Óperon Lac/genética , Masculino , Ratos , Ratos Wistar , Fator A de Crescimento do Endotélio Vascular/biossíntese
15.
Neurol Res ; 27(5): 503-8, 2005 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-15978176

RESUMO

BACKGROUND: Hypoxia-inducible factor-1 (HIF-1) is a transcription factor that regulates the adaptive response to hypoxia in mammalian cells. It consists of a regulatory subunit HIF-1alpha, which accumulates under hypoxic conditions, and a constitutively expressed subunit, HIF-1beta. In this study, we investigated HIF-1alpha naked DNA-induced angiogenesis in a cerebral ischemic model in vivo. METHODS: We utilized a rat encephalo-myo-synangiosis (EMS) model and inoculated HIF-1alpha DNA into the brain surface or the temporal muscle. We analysed whether HIF-1alpha induced angiogenic factors and collateral circulation. RESULTS: A histological section treated with HIF-1alpha DNA showed an increased expression of HIF1 a and VEGF with collateral circulation, in comparison with control DNA (p < 0.01). The HIF-1alpha transcription factor is able to promote significant angiogenesis development. CONCLUSION: These results suggest the feasibility of a novel approach for therapeutic collateral circulation of cerebral ischemia in which neovascularization may be achieved indirectly using a transcriptional regulatory strategy.


Assuntos
Isquemia Encefálica/patologia , Circulação Colateral/efeitos dos fármacos , Fatores de Transcrição/uso terapêutico , Vacinas de DNA/administração & dosagem , Animais , Vasos Sanguíneos/efeitos dos fármacos , Vasos Sanguíneos/fisiologia , Encéfalo/efeitos dos fármacos , Encéfalo/metabolismo , Encéfalo/patologia , Isquemia Encefálica/terapia , Contagem de Células/métodos , Circulação Colateral/fisiologia , DNA/imunologia , DNA/metabolismo , Modelos Animais de Doenças , Regulação da Expressão Gênica , Terapia Genética/métodos , Subunidade alfa do Fator 1 Induzível por Hipóxia , Imuno-Histoquímica/métodos , Masculino , Neovascularização Patológica/genética , Ratos , Ratos Wistar , Fatores de Transcrição/biossíntese , Fatores de Transcrição/genética , Fator A de Crescimento do Endotélio Vascular/metabolismo , beta-Galactosidase/genética , beta-Galactosidase/metabolismo
16.
J Immunol ; 174(4): 2258-64, 2005 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-15699160

RESUMO

Altered airway smooth muscle (ASM) function and enrichment of the extracellular matrix (ECM) with interstitial collagen and fibronectin are major pathological features of airway remodeling in asthma. We have previously shown that these ECM components confer enhanced ASM proliferation in vitro, but their action on its newly characterized secretory function is unknown. Here, we examined the effects of fibronectin and collagen types I, III, and V on IL-1beta-dependent secretory responses of human ASM cells, and characterized the involvement of specific integrins. Cytokine production (eotaxin, RANTES, and GM-CSF) was evaluated by ELISA, RT-PCR, and flow cytometry. Function-blocking integrin mAbs and RGD (Arg-Gly-Asp)-blocking peptides were used to identify integrin involvement. IL-1beta-dependent release of eotaxin, RANTES, and GM-CSF was enhanced by fibronectin and by fibrillar and monomeric type I collagen, with similar changes in mRNA abundance. Collagen types III and V had no effect on eotaxin or RANTES release but did modulate GM-CSF. Analogous changes in intracellular cytokine accumulation were found, but in <25% of the total ASM cell population. Function-blocking Ab and RGD peptide studies revealed that alpha2beta1, alpha5beta1, alphavbeta1, and alphavbeta3 integrins were required for up-regulation of IL-1beta-dependent ASM secretory responses by fibronectin, while alpha2beta1 was an important transducer for type I collagen. Thus, fibronectin and type I collagen enhance IL-1beta-dependent ASM secretory responses through a beta1 integrin-dependent mechanism. Enhancement of cytokine release from ASM by these ECM components may contribute to airway wall inflammation and remodeling in asthma.


Assuntos
Adjuvantes Imunológicos/fisiologia , Colágeno Tipo I/fisiologia , Citocinas/metabolismo , Fibronectinas/fisiologia , Integrina beta1/fisiologia , Pulmão/metabolismo , Músculo Liso/metabolismo , Adulto , Idoso , Anticorpos Bloqueadores/farmacologia , Anticorpos Monoclonais/farmacologia , Células Cultivadas , Quimiocina CCL11 , Quimiocinas CC/metabolismo , Citocinas/genética , Proteínas da Matriz Extracelular/fisiologia , Feminino , Regulação da Expressão Gênica/imunologia , Humanos , Integrina beta1/imunologia , Interleucina-1/fisiologia , Líquido Intracelular/imunologia , Líquido Intracelular/metabolismo , Pulmão/imunologia , Masculino , Pessoa de Meia-Idade , Músculo Liso/citologia , Músculo Liso/imunologia
17.
Clin Cancer Res ; 10(9): 3013-9, 2004 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-15131037

RESUMO

PURPOSE: We detected the relative DNA copy numbers (RCNs) at target loci in patients with stomach cancer with quantitative microsatellite analysis. We additionally clarified the relationship between DNA copy number aberrations and the clinical outcome of the patients. EXPERIMENTAL DESIGN: Fresh frozen samples were obtained from 30 patients who had undergone surgery for stomach cancer. Seven microsatellite loci in chromosomes 8q, 16q, and 20q and one gene-specific locus (ZNF217) were selected as the target loci. The DNA copy number was obtained relatively to a pooled reference consisting of six microsatellite primer sets selected from the regions where few aberrations have been reported in comparative genomic hybridization analysis. On the basis of the TaqMan PCR system, the internal probes used were carrying donor (6-carboxyfluorescein) and acceptor (6-carboxytetramethylrhodamine) fluorescent molecules complementary to CA repeats in the microsatellite markers and to one gene-specific oligomer in the gene-specific marker. RESULTS: Chromosome 8q gain, 20q gain, and 16q loss were detected in 18 (60.0%), 8 (26.7%), and 13 (43.3%) cases, respectively. Gains in the RCNs of D8S1801 and D8S1724 were most frequently found (36.7%). There was a significant correlation between the loss of D16S3026 and reduced survival duration (P = 0.0158), and the simultaneous aberrations of D8S1801 gain and D16S3026 loss (double marker positive) was significantly associated with reduced survival duration (P = 0.0008). According to Cox proportional hazards model, the double marker positive was a significant and independent factor indicating an unfavorable prognostic factor (relative risk, 17.176; 95% confidence interval, 2.782-106.026; P = 0.0022). CONCLUSION: RCN aberrations in tumor tissues determined by quantitative microsatellite analysis enable identification of the prognostic factors that correlate with clinical outcome of the patients with stomach cancer.


Assuntos
Aberrações Cromossômicas , DNA de Neoplasias/metabolismo , Repetições de Microssatélites/genética , Neoplasias Gástricas/patologia , Idoso , Idoso de 80 Anos ou mais , Cromossomos Humanos Par 16/genética , Cromossomos Humanos Par 20/genética , Cromossomos Humanos Par 8/genética , DNA de Neoplasias/genética , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Análise Multivariada , Reação em Cadeia da Polimerase , Modelos de Riscos Proporcionais , Neoplasias Gástricas/genética , Análise de Sobrevida
18.
Circ J ; 68(4): 355-60, 2004 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-15056834

RESUMO

BACKGROUND: Some patients with hypertrophic cardiomyopathy (HCM) develop left ventricular (LV) wall thinning associated with LV dilatation and systolic dysfunction. Recently, matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs (TIMPs) were reported to be involved in ventricular remodeling, however, little is known about MMPs and TIMPs in patients with HCM. METHODS AND RESULTS: Enzyme-linked immunoassays were used to measure the plasma concentrations of MMP-2, MMP-3, MMP-9, TIMP-1, and TIMP-2 in 11 patients with HCM accompanied by systolic dysfunction (fractional shortening (FS) <25%, group A), 17 patients with HCM who had preserved systolic function (FS> or =25%, group B), and 50 age-matched clinically healthy control subjects (mean age: 57 years). The concentration of MMP-2 in group A was significantly higher than in group B and the control subjects (1,124 +/- 84, 792 +/- 49, 809 +/- 26 ng/ml, respectively), whereas there was no significant difference between group B and the control subjects. MMP-2 concentrations significantly increased as the New York Heart Association functional class increased in patients with HCM. TIMP-2 was also significantly higher in group A patients than in group B and the control subjects (45.3 +/- 4.7, 34.6 +/- 2.2, 33.7 +/- 1.8 ng/ml, respectively), but there was no difference between group B and control subjects. TIMP-1 was significantly higher in HCM patients than in control subjects. MMP-3 and MMP-9 concentrations did not differ among the 3 groups. Both MMP-2 and TIMP-2 correlated significantly with FS and LV dimension, negatively and positively, respectively. CONCLUSIONS: These results suggest that changes in the release and activity of MMP-2 and TIMP-2 may be associated with the mechanisms responsible for cardiac remodeling in patients with HCM.


Assuntos
Cardiomiopatia Hipertrófica/sangue , Metaloproteinase 2 da Matriz/sangue , Inibidor Tecidual de Metaloproteinase-2/sangue , Remodelação Ventricular/fisiologia , Cardiomiopatia Hipertrófica/complicações , Cardiomiopatia Hipertrófica/fisiopatologia , Progressão da Doença , Eletrocardiografia , Ensaio de Imunoadsorção Enzimática , Feminino , Insuficiência Cardíaca/etiologia , Humanos , Masculino , Metaloproteinase 2 da Matriz/fisiologia , Pessoa de Meia-Idade , Sensibilidade e Especificidade , Sístole , Inibidor Tecidual de Metaloproteinase-1/sangue , Inibidor Tecidual de Metaloproteinase-2/fisiologia
19.
Am J Respir Crit Care Med ; 169(5): 596-603, 2004 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-14670803

RESUMO

Interleukin (IL)-13 receptor activation on airway smooth muscle cells induces eotaxin release and activates multiple signaling pathways including mitogen-activated protein kinases, and signal transducer and activator of transcription 6 (STAT6). To examine a requirement for STAT6 in mediating IL-13-stimulated eotaxin release we used antisense oligodeoxynucleotides (ODNs) to downregulate endogenous STAT6 protein. STAT6 antisense ODNs were taken up by about 85% of cells. Selective downregulation of STAT6 protein occurred with antisense ODNs, but not with sense or scrambled ODNs. Eotaxin release induced by IL-13 or IL-4 (10 ng/ml) was reduced by 81 +/- 4 and 75 +/- 7%, respectively, in cells transfected with antisense ODNs (p < 0.001), but not with a sense ODN or a scrambled ODN. Eotaxin release induced by IL-1beta was unaffected by STAT6 antisense ODN (p > 0.05). Finally, IL-13- or IL-4-dependent eotaxin release was abolished when inhibitors of both p42/p44 ERK (U0126, 10 microM) and p38 (SB202190, 10 microM) mitogen-activated protein kinase pathways were combined in STAT6 antisense ODN-transfected cells. In contrast, about 25% of the response remained when each inhibitor was examined alone in STAT6 antisense ODN-treated cells. These data support roles for both STAT6- and mitogen-activated protein kinase-dependent pathways in mediating eotaxin release from airway smooth muscle by IL-13 or IL-4.


Assuntos
Asma/imunologia , Quimiocinas/imunologia , Interleucina-13/imunologia , Músculo Liso , Mucosa Respiratória/imunologia , Transdução de Sinais/imunologia , Adulto , Idoso , Asma/prevenção & controle , Hiper-Reatividade Brônquica/imunologia , Butadienos/imunologia , Butadienos/farmacologia , Células Cultivadas , Quimiocina CCL11 , Quimiocinas CC/imunologia , Regulação para Baixo , Feminino , Humanos , Imidazóis/imunologia , Imidazóis/farmacologia , Inflamação , Interleucina-4/imunologia , Masculino , Pessoa de Meia-Idade , Proteína Quinase 1 Ativada por Mitógeno/antagonistas & inibidores , Proteína Quinase 1 Ativada por Mitógeno/imunologia , Proteína Quinase 3 Ativada por Mitógeno , Proteínas Quinases Ativadas por Mitógeno/antagonistas & inibidores , Proteínas Quinases Ativadas por Mitógeno/imunologia , Músculo Liso/citologia , Músculo Liso/imunologia , Músculo Liso/metabolismo , Nitrilos/imunologia , Nitrilos/farmacologia , Oligodesoxirribonucleotídeos Antissenso/imunologia , Piridinas/imunologia , Piridinas/farmacologia , Mucosa Respiratória/metabolismo , Fator de Transcrição STAT6 , Transativadores/imunologia , Proteínas Quinases p38 Ativadas por Mitógeno
20.
Scand J Infect Dis ; 35(8): 495-7, 2003.
Artigo em Inglês | MEDLINE | ID: mdl-14514151

RESUMO

A 1998-1999 outbreak of measles in Kawasaki City was studied to provide an up-to-date epidemiological understanding of the disease in Japan. 21 of the 69 total patients (30.4%) seen at the hospital were under 1 y of age. None of the 69 patients had received measles vaccine. 43 out of 50 nasopharyngeal swabs subjected to polymerase chain reaction assay (86%) were positive for the hemagglutinin (H) gene. The measles strain isolated from patients was classified as a D3 genotype. Such outbreaks are important epidemiologically, and these findings shed light on vaccination and immunity patterns in this urban population.


Assuntos
Surtos de Doenças/prevenção & controle , Vacina contra Sarampo/administração & dosagem , Sarampo/epidemiologia , Vacinação/normas , Adolescente , Distribuição por Idade , Criança , Pré-Escolar , Feminino , Humanos , Esquemas de Imunização , Incidência , Lactente , Japão/epidemiologia , Masculino , Estudos Retrospectivos , Fatores de Risco , Distribuição por Sexo , População Suburbana , Tóquio/epidemiologia , Vacinação/tendências
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