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1.
Head Neck Pathol ; 2021 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-34716904

RESUMO

Several attempts have been made to classify odontogenic tumors; however, the need for a uniform international classification system led the World Health Organization (WHO) to present a classification of odontogenic tumors in 1971. We aimed to evaluate the number and types of odontogenic tumors examined at the Tokyo Dental College Hospital in Japan to determine the frequency and types of odontogenic tumors, based on the 2017 WHO classification system, as this information has not been reported previously in Japan. We also compared the results of our evaluation with those reported in previous studies. We conducted a clinicopathological evaluation of odontogenic tumors examined at the Tokyo Dental College Hospital between 1975 and 2020. This included an analysis of 1089 cases (malignant, n = 10, 0.9%; benign, n = 1079, 99.1%) based on the 2017 World Health Organization Classification of Head and Neck Tumors. We identified 483 (44.3%), 487 (44.7%), and 109 (10.0%) benign epithelial odontogenic, mixed odontogenic, and mesenchymal tumors, respectively. The most common tumor types were odontoma (42.5%) and ameloblastoma (41.9%). Of the 1089 cases, 585 (53.7%) and 504 (46.3%) were male and female patients, respectively. Ameloblastoma and ameloblastic fibroma occurred more commonly in male patients, whereas odontogenic fibroma and cemento-ossifying fibroma affected female patients primarily. The age at diagnosis ranged from three to 87 (mean, 29.05) years. In 319 (29.3%) patients, the age at diagnosis ranged from 10 to 19 years. Ameloblastoma and odontoma were the most common tumor types among patients in their 20s and those aged 10-19 years, respectively. In 737 (67.7%) and 726 (66.7%) patients, the tumors were located in the mandible and posterior region, respectively. Ameloblastoma was particularly prevalent in the posterior mandible. Odontogenic tumors are rare lesions and appear to show a definite geographic variation.

2.
Radiol Case Rep ; 16(12): 3643-3646, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34630791

RESUMO

Mucoepidermoid carcinoma (MEC) of the lacrimal gland (LG) is a rare entity. A 47-year-old woman was aware of periorbital swelling for 3 months. At presentation, the patient showed periorbital swelling in the right eye. CT scan showed an isodense mass in the anterior superolateral part of the orbit. MRI delineated the mass as enhancing, extra-conal tumor appearing isointense on T1-weighted sequences, and to be of mixed intensity on T2-weighted sequences. The tumor was totally resected. Microscopically, the tumor tissue was comprised of squamous, epithelioid cells, and cells with plump and clear cytoplasm. Necrosis, neural invasion, or mitotic figures were not observed. Immunohistochemical examination revealed intense staining for cytokeratin 7. A subset of the cells was positively stained with periodic acid-Schiff and mucicarmine stains. Genetic analysis revealed the presence of the CRTC1-MAML2 fusion. The CRTC1-MAML2 fusion may be a useful indicator for the prognosis and planning of adjuvant therapy.

3.
Int J Surg Case Rep ; 83: 106019, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-34098188

RESUMO

INTRODUCTION: Static bone cavity (SBC) is a bone defect that develops as a result of localized pressure from tissues surrounding the mandible. It is most commonly observed in the mandibular angle of adult males caused by the submandibular gland. The condition is asymptomatic and requires no treatment. The frequency of onset is rare, especially in the anterior mandible, and SBC is extremely difficult to diagnose in children. PRESENTATION OF CASE: This report is on a case of SBC in the anterior mandible of a 10-year-old boy. The condition could not be diagnosed after panoramic radiograph and Magnetic Resonance Imaging (MRI) analyses. Computed-topography (CT) imagery revealed an oval-shaped depression 6 × 5 × 3 mm in size at the lingual apex of the mandibular left lateral tooth. Diagnosing the patient was difficult and a tumor was suspected. For treatment, the tumor-like lesion was resected and the fistula in the periosteum was sutured and closed. One year later, the defect on the lingual mandibular bone had ossified and recovered. CONCLUSION: The incidence of SBC in children is rare and the condition is difficult to diagnose because of the small size of the lesions, however in this study, CT imagery proved to be useful. SBC was detected in the subject in early childhood, and his postoperative course suggested that the lesion developed as a result of glandular tissues herniating through the periosteum and causing compression on the mandible, which resulted in bone resorption. In other words, herniation of normal salivary glandular tissues were a cause of SBC.

4.
Clin Exp Dent Res ; 7(3): 344-353, 2021 06.
Artigo em Inglês | MEDLINE | ID: mdl-33783980

RESUMO

OBJECTIVES: Transplantation of stem cells into wounds has become popular in regeneration therapies. As stem cells for transplantation, human dental pulp stem cells (hDPSCs) are known to be pluripotent cells that are relatively easy to collect from the pulp of deciduous or wisdom teeth. The purpose of this study was to investigate whether hDPSCs treated with fibroblast growth factor 7 (FGF7) would contribute to the regeneration of wounded rat submandibular glands (SMGs). MATERIALS AND METHODS: In in vitro studies, hDPSCs were treated with or without FGF7 and mRNA expression levels were examined at days 3, 7 and 14 using qRT-PCR. The target genes analyzed were BMI1 as an undifferentiated marker, AQP5 as an acinar cell marker, CK19 as a ductal epithelial cell marker, αSMA as a myoepithelial cell marker and VIMENTIN as a fibroblast marker. In in vivo studies, hDPSCs treated with or without FGF7 for 14 days were mixed with type I collagen gels and were transplanted into wounded rat SMGs. Hematoxylin-Eosin and immunohistochemical staining were performed at days 3 and 7, and the numbers of positive cells were counted. The primary antibodies used were against BMI1, AQP5, αSMA, PanCK and VIMENTIN. RESULTS: In the in vitro studies, mRNA levels of BMI1 were decreased and αSMA were increased at days 3, 7 and 14, while AQP5 was increased at day 14 in the FGF7 group. In the in vivo studies, the proliferation of hDPSCs and cell islands was observed at day 7 in the FGF7 group. Few BMI1-positive cells were observed, while numbers of AQP5-positive and αSMA-positive cells were increased at days 3 and 7 in the FGF7 group. Moreover, cell islands were AQP5-positive. CONCLUSION: These results suggest that FGF7-treated hDPSCs differentiate into AQP5-positive and αSMA-positive cells. Moreover, AQP5-positive cell aggregations were formed.

5.
Oral Radiol ; 37(2): 336-344, 2021 04.
Artigo em Inglês | MEDLINE | ID: mdl-32909103

RESUMO

Sialolithiasis is a common salivary pathology, and an uncommon complication of sialadenitis and sialolithiasis is the formation of fistulous tracts to other compartments. Submandibular gland sialo-oral fistulae are not particularly remarkable, given the location of the gland and Wharton's duct, but submandibular sialolith-associated fistulae to other cervico-facial compartments (transcervical sialo-cutaneous and sialo-pharyngeal fistulae) are much less common. We report herein an unusual case of a 49-year-old obese man with sialo-cutaneous fistula containing a large, ectopic sialolith in subcutaneous tissue that was expected to undergo spontaneous elimination, but revealed hidden Eagle syndrome featuring an ipsilateral enlarged, elongated styloid process. Furthermore, we offer a thorough review of the literature regarding sialo-fistulae and highlight the relationship between an abnormal styloid process and submandibular sialadenitis with sialolithiasis and new tract formation based on computed tomography.


Assuntos
Fístula Cutânea , Cálculos das Glândulas Salivares , Doenças da Glândula Submandibular , Fístula Cutânea/diagnóstico por imagem , Fístula Cutânea/etiologia , Humanos , Masculino , Pessoa de Meia-Idade , Cálculos das Glândulas Salivares/diagnóstico por imagem , Glândula Submandibular/diagnóstico por imagem , Doenças da Glândula Submandibular/diagnóstico por imagem , Doenças da Glândula Submandibular/etiologia , Tomografia Computadorizada por Raios X
6.
Clin Exp Dent Res ; 7(2): 205-210, 2021 04.
Artigo em Inglês | MEDLINE | ID: mdl-33274608

RESUMO

OBJECTIVES: Epigenetic phenomena are changes in gene expression not involving the DNA sequence. DNA methylation is a major occurrence underlying epigenetic changes in human cells. Although aberrant DNA methylation is well documented in malignant lesions, limited information has been shown on the involvement of DNA methylation in oral lichen planus and oral lichenoid lesions (OLP). The present study aimed to investigate DNA methylation of E-cadherin and p16 in OLP, and compare the findings with those in non-inflamed gingiva (Non), radicular cyst (RC), and oral squamous cell carcinoma (SCC). MATERIALS AND METHODS: Paraffin-embedded surgical biopsy specimens were sliced, DNA was extracted, bisulfite treatment was applied, and methylation-specific polymerase chain reaction was performed. Immunohistochemistry was performed to observe the relative expression patterns of these genes. RESULTS: E-cadherin was hypermethylated in OLP (p < 0.01), SCC (p < 0.01), and RC (p < 0.05), when compared with Non; DNA hypermethylation was confirmed in OLP and SCC when compared to Non and RC. Hypermethylation of p16ink4a was observed only in SCC (p < 0.01). CONCLUSION: DNA methylation levels of E-cadherin and p16ink4a were significantly higher in OLP than in normal tissues, and may be associated with the pathogenesis and progression of the disease.

7.
Pathol Int ; 71(2): 113-123, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33333616

RESUMO

Diagnostic utility of a homeobox transcription factor, engrailed homeobox 1 (En1) in the histopathology of salivary gland neoplasms was studied. The expression of En1 was immunohistochemically examined in 51 cases of adenoid cystic carcinoma (AdCC) and 143 cases of other salivary gland neoplasms. In all 51 AdCCs, En1 was expressed in 30-100% of tumor cells. In eight of nine polymorphous adenocarcinomas (PACs), En1 was expressed in 40-100% of tumor cells. Less than 5% of tumor cells expressed En1 in three of 12 epithelial-myoepithelial carcinomas, one of 17 basal cell adenomas (BCAs), and one of 34 pleomorphic adenomas (PAs). Among 55 other carcinoma cases, 1-30% of tumor cells expressed En1 in three salivary duct carcinomas (SDCs) ex PA. None of the myoepitheliomas and Warthin tumors expressed En1. When the cut-off value of the percentage of En1-expressing cells was set to 25%, all 51 AdCCs, eight of nine PACs and one SDC ex PA were En1-positive and the others were En1-negative. En1 is expressed consistently in AdCCs, frequently in PACs, but rarely in other salivary gland neoplasms. En1 is a possible diagnostic marker for AdCC and PAC in the histopathology of salivary gland neoplasms.


Assuntos
Biomarcadores Tumorais/metabolismo , Carcinoma Adenoide Cístico/diagnóstico , Proteínas de Homeodomínio/metabolismo , Neoplasias das Glândulas Salivares/diagnóstico , Adenoma/diagnóstico , Adenoma/metabolismo , Adenoma/patologia , Adenoma Pleomorfo/diagnóstico , Adenoma Pleomorfo/metabolismo , Adenoma Pleomorfo/patologia , Carcinoma Adenoide Cístico/metabolismo , Carcinoma Adenoide Cístico/patologia , Diagnóstico Diferencial , Humanos , Imuno-Histoquímica , Curva ROC , Neoplasias das Glândulas Salivares/metabolismo , Neoplasias das Glândulas Salivares/patologia , Sensibilidade e Especificidade
8.
Oncol Lett ; 20(3): 2153-2160, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32782532

RESUMO

Disorders of the oral mucosa are considered easy to diagnose since they can be visualized and examined directly. A change in the color of the oral mucosa reflects histopathological changes and is an important diagnostic parameter. However, the subjective perception of color varies. To determine the extent of resection for oral mucosa conditions, it is necessary to digitize the color and perform objective assessments. In recent years, fluorescence visualization devices and analysis software that measure tissue luminance G have been employed for the identification of oral mucosa diseases. Fluorescence visualization is presumably based on the decrease in epithelial flavin adenine dinucleotide content and luminance G values due to the destruction of collagen cross-links [fluorescence visualization loss (FVL)]. However, cases with differences between luminance values and histopathological presentation exist. Therefore, additional factors may affect fluorescence visualization. The present study used a portable, non-contact oral mucosa fluorescence visualization device for luminance measurements in seven patients with oral squamous cell carcinoma. Furthermore, Picro-Sirius Red and immunohistochemical staining were performed for CK13, CK17, Ki67, p53 and E-cadherin in the FVL(+) (lesion) and FVL(-) (resection stump) areas to elucidate the principle of fluorescence visualization. Fluorescence was significantly lower in the FVL(+) than in the FVL(-) areas, and the mean luminance G value was 56. The Picro-Sirius Red stain revealed collagen destruction in the FVL(+) areas but no collagen disruption in the FVL(-) areas. CK13 was negative in the FVL(+) and positive in the FVL(-) areas, whereas the opposite pattern was observed for CK17. In the FVL(+) area, p53 staining was positive. E-cadherin expression was enhanced in the FVL(-) areas and reduced in the FVL(+) areas. Furthermore, the luminance G value tended to be lower in cases with weaker E-cadherin staining. The aforementioned results suggest that decreased E-cadherin expression may be a factor that regulates fluorescence visualization.

9.
Bull Tokyo Dent Coll ; 61(1): 61-69, 2020 Mar 12.
Artigo em Inglês | MEDLINE | ID: mdl-32074583

RESUMO

We report herein a case of a luminal and intramural unicystic ameloblastoma (UA) with a marked fluid-fluid level. The validity of imaging findings in diagnosing UA in the present case is discussed in reference to the literature. The patient was a 50-year-old woman who presented with swelling of the gingiva in the region of the left mandibular third molar and numbness in the lower lip. Computed tomography (CT) and magnetic resonance imaging (MRI) revealed a large mass lesion with a unilocular appearance and a biphasic aspect, suggesting liquid content. Contrast-enhanced MRI (CE-MRI) and dynamic contrast-enhanced MRI (DCE-MRI) demonstrated that the biphasic aspect indicated a fluid-fluid level with no blood pooling/flow; it also revealed a thick rim-enhanced margin with mural protrusion. Postoperatively, the lesion was histopathologically diagnosed as a luminal and intramural UA. In conclusion, extensive imaging including both standard CT and MRI together with CE-MRI and DCE-MRI allowed mural protrusions or nodules on a thick cystic wall and liquid content to be correctly identified. This suggests that such imaging can play an important role in diagnosing a UA, even though the results were at first misleading due to the marked fluid-fluid level.


Assuntos
Ameloblastoma , Feminino , Humanos , Imageamento por Ressonância Magnética , Pessoa de Meia-Idade , Tomografia Computadorizada por Raios X
10.
Bull Tokyo Dent Coll ; 60(4): 267-277, 2019 Dec 10.
Artigo em Inglês | MEDLINE | ID: mdl-31761877

RESUMO

The purpose of this study was to investigate the behavior of epithelial lining derived from Malassez's epithelial rest (MER) cells in experimentally created inflammatory cysts in vivo and in vitro. Porcine MER cells were cultured in vitro with or without interleukin (IL)-1ß (1 ng/ml) or IL-6 (1 ng/ml). Cell proliferation was assessed and expression levels of CK19 and CK13 mRNA determined using RT-PCR. In vivo, a cavity was created in the first molar of Sprague-Dawley male rats and tissue repair observed using immunohistochemical methods. In vitro, treatment with IL-1ß or IL-6 increased proliferation of MER cells and decreased expression of CK19 mRNA, but increased CK13 mRNA at day 1 (p<0.05). In vivo, at 2 weeks, CK19-positive epithelial cells were observed adjacent to the cementum, in the cystic lesion, and in connective tissue. At 3 weeks, they were only detected in cells adjacent to the connective tissue. Cells positive for CK13 were observed throughout the epithelium, except in cells adjacent to connective tissue at weeks 2 and 3. Exposure to IL-1ß and/or IL-6 induced proliferation and differentiation of MER cells.


Assuntos
Cistos , Queratinas , Animais , Células Epiteliais , Masculino , RNA Mensageiro , Ratos , Ratos Sprague-Dawley , Suínos
11.
Dent Mater J ; 38(5): 830-838, 2019 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-31341145

RESUMO

To investigate the effect of EDTA-treated dentin on the differentiation of mouse induced pluripotent stem (iPS) cells. Dentin discs were prepared from bovine incisors and treated with 17% EDTA. Embryoid bodies (EBs) formed from mouse iPS cells were seeded on the dentin discs for the experiment. The roughness of the EDTA-treated dentin surface, Sa and Sdr, was higher and collagen fibrillike structures were observed by the scanning electron microscopy (SEM) in vitro. In RT-PCR, the mRNA levels of the osteoblast markers Bsp and Ocn were significantly higher in the experimental group. Expression of the DMP1, DSP, and BSP proteins were more notable in the experimental group by immunofluorescence (ICF) study. In vivo study, cartilage and bone-like tissue were observed adjacent to the EDTA-treated dentin. The study demonstrates that the dentin treated with 17% EDTA induces mouse iPS cells to differentiate into the osteo/odontogenesis.


Assuntos
Células-Tronco Pluripotentes Induzidas , Animais , Bovinos , Diferenciação Celular , Células Cultivadas , Dentina , Ácido Edético , Camundongos , Odontogênese
12.
Bull Tokyo Dent Coll ; 60(1): 29-37, 2019 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-30700642

RESUMO

Oral exfoliative cytology is now used by general practitioners in Japan to screen for oral cancer. With conventional cytology, however, the number of cells that can be sampled is small. Moreover, cell deformation and piling of cells when preparing specimens has been reported. The purpose of this study was to compare conventional and liquid based cytology (LBC), which has been employed with increasing frequency in recent years. We believe that identifying potential pitfalls in oral exfoliative cytology will help improve diagnostic accuracy. A total of 153 patients with tongue squamous cell carcinoma who were diagnosed and treated initially at our hospital between January 2000 and December 2010 were included. Of these, 124 underwent conventional cytology, while the remaining 29 underwent LBC. Histopathological and clinical findings were used as criteria. Conventional cytology yielded a positive rate of 54.8% and LBC 79.3%, while values of 28.2% and 13.8% were obtained for a suspected positive rate, respectively. Liquid based cytology yielded a significantly higher percentage of accurate diagnoses and fewer suspected positives (p<0.05) in cases clinically classified as endophytic and those classified as ulcerative in terms of clinical growth pattern. No significant difference was observed between conventional cytology and LBC in cases of an infiltrative growth pattern, however.The present results suggest that LBC is superior to conventional cytology in achieving an accurate diagnosis based on oral exfoliative cytology. The present findings also suggest that exophytic type, and especially leukoderma type clinical growth patterns constitute pitfall cases in oral exfoliative cytology.


Assuntos
Carcinoma de Células Escamosas/diagnóstico , Técnicas Citológicas/métodos , Detecção Precoce de Câncer/métodos , Neoplasias da Língua/diagnóstico , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Sensibilidade e Especificidade , Adulto Jovem
13.
J Biomed Mater Res B Appl Biomater ; 107(7): 2288-2296, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-30735289

RESUMO

The effect of differing polystyrene substrate topographies on the osteogenic potential of the outgrowing cells (OGCs) formed from mouse-induced pluripotent stem (iPS) cells (miPSCs)-derived embryoid bodies (EBs) was investigated. Polystyrene substrates were sandblasted with 25, 50, and 150 µm aluminum oxide particles to obtain topographies with average Sa values of 0.6, 1.1, and 1.8 µm, respectively. 3D-SEM was used to evaluate substrate's topographies. Examination was done by scanning electron microscopy (SEM), by immunocytofluorescence (ICF) analysis for vinculin, Runx2 and collagen type I, and by quantitative RT-PCR (qRT-PCR) analysis for Runx2 and collagen type I. SEM and ICF analyses revealed that surface roughness caused cells elongation (2, 6, 8, 10 times for the NT, 0.6 µm, 1.1 µm, and 1.8 µm, respectively). Vinculin staining demonstrated how the Sa value affected cellular attachment to the substrate. FA points were randomly distributed on flat surfaces, but rough surfaces resulted in more concentrated FA points on the podia of the cells (11.7, 25.2, 26.7, 16.6 vinculin spots per 20 µm2 for the NT, 0.6 µm, 1.1 µm, and 1.8 µm, respectively). qRT-PCR revealed that Runx2 expression was highest on day 16 on surfaces with Sa of 0.6 µm and 1.1 µm. Collagen type I expression increased from day 0 to day 16, no significance was found among the groups. In conclusion, surface topography affects cell shape and expression of early osteogenic potential in OGC, particularly surfaces with Sa values of 0.6 µm and 1.1 µm which showed the highest concentration of FA points on podia. These findings could be utilized in the development of inner surface topographies of scaffolds used with iPSCs. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 2288-2296, 2019.


Assuntos
Subunidade alfa 1 de Fator de Ligação ao Core/biossíntese , Corpos Embrioides/metabolismo , Regulação da Expressão Gênica , Células-Tronco Pluripotentes Induzidas/metabolismo , Poliestirenos/química , Animais , Corpos Embrioides/citologia , Células-Tronco Pluripotentes Induzidas/citologia , Camundongos
14.
Bull Tokyo Dent Coll ; 59(3): 207-212, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30224615

RESUMO

On images, a dermoid cyst is often described as resembling a "sack of marbles" or "marbles in a bag". Typically, it comprises an inhomogeneity filled with multiple nodules in a fluid matrix on both computed tomography and magnetic resonance imaging (MRI). How it appears, however, will vary depending on its histological contents, which may cause confusion in arriving at a diagnosis. This report describes a dermoid cyst in the floor of the mouth of a 55 year-old woman that showed an atypical internal appearance on MRI. Most of the lesion showed homogeneous high signal intensity on T1 - and T2-weighted images, suggesting that it was derived from fat. A small area within the mass, however, showed moderate signal intensity almost equal to that of muscle on T1-weighted images and high signal intensity on fat-suppressed T2-weighted images. Given the location of the lesion, a dermoid cyst was one possible diagnosis. A lipoma or lipoma variants were also considered, however, based on signal intensity. Histopathological section of the excised specimen revealed a dermoid cyst with sebaceous glands in its walls and keratin in its cavity. Dermoid cysts show variation in their internal structures and contents. Since MRI can reflect such histological variation, signal intensity requires careful interpretation.


Assuntos
Cisto Dermoide/diagnóstico por imagem , Cisto Dermoide/patologia , Soalho Bucal/diagnóstico por imagem , Neoplasias Bucais/diagnóstico por imagem , Neoplasias Bucais/patologia , Feminino , Humanos , Imageamento por Ressonância Magnética , Pessoa de Meia-Idade
15.
Bull Tokyo Dent Coll ; 59(2): 127-132, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29962420

RESUMO

Congenital epulis is an unusual benign oral mucosal lesion in newborns with no tendency to recur after excision. The histogenesis of the lesion is unknown, but it is believed to be of mesenchymal origin. We describe a case of congenital epulis (20×10 mm) in the mandibular gingiva of a newborn. The mass, which was smooth-surfaced and pedunculated with a healthy color, was surgically removed at 5 months post-birth. Histologically, the tumor consisted mainly of large eosinophilic granular cells. Immunohistochemical studies revealed intense staining for vimentin, STRO-1, and CD44, suggesting that it was derived from mesenchymal cells. The literature and immunohistochemical profile of congenital epulis are also discussed.


Assuntos
Neoplasias Gengivais/patologia , Neoplasias Mandibulares/patologia , Antígenos de Superfície/análise , Biomarcadores Tumorais/análise , Diagnóstico Diferencial , Feminino , Gengiva/patologia , Neoplasias Gengivais/cirurgia , Humanos , Receptores de Hialuronatos/análise , Imuno-Histoquímica , Recém-Nascido , Mandíbula/patologia , Mandíbula/cirurgia , Neoplasias Mandibulares/química , Neoplasias Mandibulares/cirurgia
16.
Dent Mater J ; 36(1): 103-110, 2017 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-28090031

RESUMO

In the present report, we have generated osteoblast-like cells derived from mouse induced-pluripotent stem (iPS) cells on PLGA with osteoinduction medium in vitro and in vivo. The cell culture period was 2 weeks. At 2 weeks, mRNA level of type I collagen was significantly higher than at 1 week. Osteocalcin mRNA level at 2 weeks was tendency to increase compared with at 1 week. And the cells cultured on PLGA were positive for immunofluorescent staining of osteocalcin and alizarin red S staining. The scaffold and osteogenic-like cells induced in vitro were implanted subcutaneously into SCID mice. In resected teratoma, hard tissues resembling bone were observed mixed with other tissues on the scaffold. The sum of these findings suggests that PLGA does not disturb the osteogenesis of iPS cells.


Assuntos
Diferenciação Celular , Células-Tronco Pluripotentes Induzidas , Osteogênese , Animais , Células Cultivadas , Células-Tronco Mesenquimais , Camundongos , Camundongos SCID
17.
Arch Oral Biol ; 70: 158-164, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27371807

RESUMO

OBJECTIVE: This study investigated the expression of receptor activator of nuclear factor-κB ligand (RANKL) in periodontal ligament (PDL) cells co-cultured with dental pulp (DP) cells following mechanical stress in vitro. Furthermore, the expression of prostaglandin (PG) E2 and substance P (SP) by the PDL cells and by the DP cells were also examined. DESIGN: PDL and DP cells were obtained from 10 rats. The experimental group consisted of PDL cells subjected to centrifugal force as mechanical stress and co-cultured with DP cells. The 3 control groups of PDL cells were: 1) PDL cells without mechanical stress, 2) PDL cells treated with mechanical stress and 3) PDL cells co-cultured with DP cells. The 2 control groups of DP cells were: 1) DP cells without mechanical stress and 2) DP cells co-cultured with PDL cells. In each group, both cells were examined at day 1 and day 3, and mRNA levels of RANKL by PDL cells were analyzed using Real time quantitative Reverse Transcription (RT)-PCR. Furthermore, RANKL expression was observed using Immunofluorescence staining. PGE2 and SP expression levels by PDL cells and DP cells were characterized by ELISA analysis. RESULTS: The expression of RANKL by PDL cells under mechanical stress increased by co-culture with DP cells. PGE2 and SP expressions were increased in the group of PDL cells subjected to mechanical stress and co-cultured with DP cells. CONCLUSION: DP cells may facilitate the expression of RANKL in PDL cells under mechanical stress via PGE2 and SP.


Assuntos
Polpa Dentária/citologia , Polpa Dentária/metabolismo , Ligamento Periodontal/citologia , Ligamento Periodontal/metabolismo , Prostaglandinas/biossíntese , Ligante RANK/biossíntese , Substância P/biossíntese , Animais , Células Cultivadas , Técnicas de Cocultura , Citocinas/biossíntese , Citocinas/metabolismo , Polpa Dentária/diagnóstico por imagem , Ensaio de Imunoadsorção Enzimática , Células Epiteliais/metabolismo , Masculino , Microscopia Confocal , Ligante RANK/genética , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Ratos , Ratos Sprague-Dawley , Reação em Cadeia da Polimerase em Tempo Real , Estresse Mecânico , Substância P/genética
18.
Int J Oral Sci ; 8(1): 16-23, 2016 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-27025261

RESUMO

This study developed an animal model of surgically wounded submandibular glands (SMGs) and investigated the effects of collagen gel with basic fibroblast growth factor (bFGF) on tissue regeneration of surgically wounded SMGs in vivo. The animal model was produced by creating a surgical wound using a 3-mm diameter biopsy punch in SMGs. The wound was filled with collagen gel with bFGF (bFGF group) or without bFGF (control group). In the animal model of surgically wounded SMGs, salivary glands without scar tissue around the wound area were observed with smaller areas of collagen gel. Small round and spindle-shape cells invaded the collagen gel in both groups after operation day (AOD) 5, and this invasion dramatically increased at AOD 7. Host tissue completely replaced the collagen gel at AOD 21. The invading immune cells in the group treated with collagen gel with bFGF were positive for vimentin, α-smooth muscle actin (αSMA), CD49f, c-kit and AQP5 at AOD 7. Similarly, the mRNA expression of vimentin, αSMA, CD49f, keratin19 and AQP5 was also increased. This study suggests that the use of collagen gels with bFGF improves salivary gland regeneration.


Assuntos
Fator 2 de Crescimento de Fibroblastos/farmacologia , Regeneração/efeitos dos fármacos , Glândula Submandibular/cirurgia , Cicatrização/efeitos dos fármacos , Animais , Colágeno/farmacologia , Modelos Animais de Doenças , Géis , Imuno-Histoquímica , Masculino , Ratos , Ratos Sprague-Dawley , Reação em Cadeia da Polimerase Via Transcriptase Reversa
19.
Photomed Laser Surg ; 34(4): 157-63, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26982347

RESUMO

OBJECTIVE: The purpose of this study was to investigate the effects of CO2 lasers on the proliferation and differentiation of dental pulp cells, and their latent self-recovery in connection with their stemness using reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry. MATERIALS AND METHODS: The first molars from male Sprague-Dawley rats, each weighing ∼150-200 g, were used for this study. The upper first molars were irradiated with a 10,600 nm wavelength CO2 laser under identical parameters (2 W CO2 laser, energy 4J, energy density 203.84 J/cm(2) for 8.8 sec) through the dentin of the occlusal surface. The molars were extracted immediately, or at 1, 3 or 5 days after the laser irradiation. RT-PCR analysis using primers specific for heat shock protein 70 (Hsp70), adenosine triphosphate (ATP)-binding cassette transporter G2 (ABCG2), dentin sialophosphoprotein (DSPP), and dentin matrix protein 1 (DMP1), and immunohistochemistry using antibodies specific for proliferating cell nuclear antigen (PCNA), ABCG2, CD34, and CD44 were performed. RESULTS: RT-PCR analysis revealed that Hsp70 mRNA expression in the immediate group and ABCG2 mRNA expression at day 1 were the highest. DSPP and DMP1 mRNA expression in the laser-irradiated groups increased gradually, reaching its peak on the 5th day of the experiment, although no significant difference found among groups with regard to DMP1 expression. Immunohistochemically, PCNA-positive cells were observed at all times after the laser irradiation; however, they were most evident on day 3. CD44-positive cells were observed strongly on day 1 and day 3, while ABCG2-positive cells were the most evident on day 3. CONCLUSIONS: These results demonstrate that CO2 laser irradiation induces degeneration in the pulp tissue, which is then repaired by newly formed odontoblast-like cells.


Assuntos
Polpa Dentária/citologia , Lasers de Gás , Células-Tronco Mesenquimais/efeitos da radiação , Animais , Diferenciação Celular/efeitos da radiação , Proliferação de Células/efeitos da radiação , Polpa Dentária/efeitos da radiação , Masculino , Modelos Animais , Odontoblastos/efeitos da radiação , Ratos , Ratos Sprague-Dawley
20.
BMC Oral Health ; 16: 22, 2016 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-26888092

RESUMO

BACKGROUND: The purpose of the present study was to examine the in vitro responses of ERM cells under the combination of centrifugal and compression forces, in terms of their expression of HSP70 mRNA. METHODS: The ERM cells were positive for CK19 indicating that they were derived from the odontogenic epithelium. Cultured ERM cells were applied centrifugal force and compressing force at one to three times as mechanical forces. After addition of forces, cells were observed using scanning electron microscope (SEM) and were measured expression of HSP70 mRNA by RT-PCR. RESULTS: SEM observations showed the cells were flattened immediately after the application of mechanical force, but nuclear protrusions recovered the same as the control 3 h later. A significantly higher expression of HSP70 mRNA was observed in ERM cells under mechanical force compared with the control, but it gradually decreased with time. No accumulation of HSP70 mRNA expression occurred with intermittent force. However, the expression of HSP70 mRNA with intermittent force repeated 3 times was significantly higher compared with intermittent force applied only once or twice. CONCLUSIONS: These findings suggest that ERM cells express HSP70 mRNA in response to mechanical force, and that intermittent force maintains the level of HSP70 mRNA expression.


Assuntos
Células Epiteliais/metabolismo , Proteínas de Choque Térmico HSP70/metabolismo , Microscopia Eletrônica de Varredura , Animais , Células Cultivadas , Células Epiteliais/ultraestrutura , RNA Mensageiro/metabolismo , Estresse Mecânico , Suínos
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