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1.
J Cell Physiol ; 2021 Feb 02.
Artigo em Inglês | MEDLINE | ID: mdl-33533043

RESUMO

Aberrant expression of CD43 in malignant tumors of nonhematopoietic origin such as those from lung, cervix, colon, and breast has been shown to correlate with poor prognosis, providing tumor cells with enhanced motility, anchorage-independent growth, and in vivo tumor size, while protecting the cells of NK lysis and apoptosis. To further characterize the role of CD43 in cell transformation, we tested whether interfering its expression modified the capacity of the A549 non-small cell lung cancer cells to secrete molecules contributing to malignancy. The proteomic analysis of the secretome of serum-starved A549 cells revealed that cells expressing normal levels of CD43 released significantly high levels of molecules involved in extracellular matrix organization, angiogenesis, platelet degranulation, collagen degradation, and inflammation, as compared to CD43 RNAi cells. This data reveals a novel and unexpected role for CD43 in lung cancer development, mainly in remodeling the tumor microenvironment.

2.
BMC Res Notes ; 13(1): 364, 2020 Aug 03.
Artigo em Inglês | MEDLINE | ID: mdl-32746904

RESUMO

OBJECTIVE: To determine if fermented soy supplementation relieves heartburn and improves gastrointestinal symptoms and quality of life, a randomized, double-blind parallel study was conducted with adults experiencing mild or moderate heartburn. Participants consumed up to 3, 1 g sachets of flavored, Lactobacillus delbrueckii fermented with soy flour (n = 23) or placebo (maltodextrin) (n = 27) sachets per heartburn incident as needed for 3 weeks. Symptom intensity at 5, 15, and 30 min post-administration was assessed using a Likert-like scale. The Gastrointestinal Symptoms Rating Scale (GSRS) and Gastro-esophageal Reflux Disease Quality of Life Questionnaire (GERD-QOL) were administered at baseline, post-intervention and following a 1-week washout. RESULTS: No significant differences between groups were seen for heartburn severity or frequency, GSRS syndromes, or GERD-QOL domains. However, individual QOL items related to inconvenience of taking medications, fear of eating, inability to concentrate at work, and disturbance of after-meal activities and rest improved with fermented soy compared to placebo. Frequency of heartburn, diarrhea, and bloating improved during washout vs. baseline for the fermented soy group compared to placebo. Lactobacillus delbrueckii fermented soy supplementation improved QOL indicators and may decrease heartburn occurrence over time vs. an acute effect; efficacy of daily intake and longer duration requires investigation.

3.
ChemSusChem ; 13(23): 6360-6369, 2020 Dec 07.
Artigo em Inglês | MEDLINE | ID: mdl-32672415

RESUMO

A straightforward procedure has been developed to prepare a porous carbon material decorated with iron by direct pyrolysis of a mixture of a porous polymer and iron chloride. Characterization of the material with X-ray diffraction, X-ray absorption spectroscopy, and electron microscopy indicates the presence of iron carbide nanoparticles encapsulated inside the carbon matrix, and elemental mapping and cyanide poisoning experiments demonstrate the presence of atomic Fe centers, albeit in trace amounts, which are active sites for electrochemical CO2 reduction. The encapsulated iron carbide nanoparticles are found to boost the catalytic activity of atomic Fe sites in the outer carbon layers, rendering the material highly active and selective for CO2 reduction, although these atomic Fe sites are only present in trace amounts. The target material exhibits near-unity selectivity (98 %) for CO2 -to-CO conversion at a small overpotential (410 mV) in water. Furthermore, the material holds potential for practical application, as a current density over 30 mA cm-2 and a selectivity of 93 % can be achieved in a flow cell.

4.
Prog Mol Biol Transl Sci ; 171: 237-263, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32475524

RESUMO

Although there is associative evidence linking fecal microbiome profile to health and disease, many studies have not considered the confounding effects of dietary intake. Consuming food provides fermentable substrate which sustains the microbial ecosystem that resides with most abundance in the colon. Western, Mediterranean and vegetarian dietary patterns have a role in modulating the gut microbiota, as do trending restrictive diets such the paleolithic and ketogenic. Altering the amount or ratio of carbohydrate, protein and fat, particularly at the extremes of intake, impacts the microbiome. Diets high in fermentable carbohydrates support the relative abundance of Bifidobacterium, Prevotella, Ruminococcus, Dorea and Roseburia, among others, capable of degrading polysaccharides, oligosaccharides and sugars. Conversely, very high fat diets increase bile-resistant organisms such as Bilophila and Bacteroides. Food form, whole foods vs. ultra-processed, alters the provision of macronutrient substrate to the colon due to differing digestibility, and thereby may impact the microbiota and its metabolic activity. In addition, phytochemicals in plant-based foods have specific and possibly prebiotic effects on the microbiome. Further, food ingredients such as certain low-calorie sweeteners enhance Bifidobacterium spp. The weight of evidence to date suggests a high level of interindividual variability in the human microbiome vs. clearly defined, dietary-induced profiles. Healthful dietary patterns, emphasizing plant foods high in microbial-available carbohydrate, support favorable microbiome profiles active in saccharolytic fermentation. Future research into diet and microbiome should consider the balance of gut microbial-generated metabolites, an important link between microbiome profile and human health.

5.
Curr Dev Nutr ; 4(2): nzaa005, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32025615

RESUMO

Background: Consuming foods with added fiber may help older adults achieve fiber recommendations; however, many high-fiber ingredients have little effect on laxation and may contribute to unpleasant gastrointestinal side effects. Objectives: The aim of the study was to determine the effects of consuming snacks fortified with pea hull fiber (PHF) on stool frequency and form, gastrointestinal symptoms, and appetite in older adults. An exploratory aim was to determine if PHF altered the microbiota profile. Methods: A 10-wk, randomized, blinded, crossover study was carried out. Following a 2-wk baseline period, participants [aged (mean ± SD) 69.7 ± 6.5 y; n = 31; 14 men, 17 women] consumed snacks providing 10 g/d of PHF or a control, each for 2-wk periods followed by 2-wk washouts. Participants used the Bristol Stool Form Scale (BSFS) to record daily stool frequency and gastrointestinal symptoms, and completed the Gastrointestinal Symptom Rating Scale (GSRS) and Simplified Nutritional Appetite Questionnaire (SNAQ) biweekly. One stool was collected per period for 16S ribosomal RNA high-throughput amplicon sequencing of the fecal microbiota profile. Results: Participants reported 1.63 ± 0.05 stools/d and 76.6% normal transit stool form at baseline and no change with PHF. GSRS syndrome scores were similarly unchanged. Daily abdominal noises and bloating were higher for PHF versus control, and flatulence was higher for PHF versus baseline, suggesting fermentation in some individuals. There was no evidence to suggest a common PHF-induced microbiome response for the group as a whole; however, a subgroup of participants (n = 7) who responded with increased flatulence (fermenters), harbored many different taxa than nonfermenters, and demonstrated lower abundance of Clostridiales with PHF. Appetite was unchanged with PHF. Conclusions: PHF did not modulate stool form or frequency in older adults with normal bowel habits. Because snacks fortified with PHF did not suppress appetite, PHF may be an appropriate fiber source for older adults at nutritional risk. Microbiome profile may be predictive of gastrointestinal symptom response to PHF. This trial was registered at www.clinicaltrials.gov as NCT02778230.

6.
Angew Chem Int Ed Engl ; 58(45): 16172-16176, 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31496012

RESUMO

Conversion of CO2 into valuable molecules is a field of intensive investigation with the aim of developing scalable technologies for making fuels using renewable energy sources. While electrochemical reduction into CO and formate are approaching industrial maturity, a current challenge is obtaining more reduced products like methanol. However, literature on the matter is scarce, and even more for the use of molecular catalysts. Here, we demonstrate that cobalt phthalocyanine, a well-known catalyst for the electrochemical conversion of CO2 to CO, can also catalyze the reaction from CO2 or CO to methanol in aqueous electrolytes at ambient conditions of temperature and pressure. The studies identify formaldehyde as a key intermediate and an unexpected pH effect on selectivity. This paves the way for establishing a sequential process where CO2 is first converted to CO which is subsequently used as a reactant to produce methanol. Under ideal conditions, the reaction shows a global Faradaic efficiency of 19.5 % and chemical selectivity of 7.5 %.

7.
Nat Commun ; 10(1): 3602, 2019 Aug 09.
Artigo em Inglês | MEDLINE | ID: mdl-31399585

RESUMO

Molecular catalysts that combine high product selectivity and high current density for CO2 electrochemical reduction to CO or other chemical feedstocks are urgently needed. While earth-abundant metal-based molecular electrocatalysts with high selectivity for CO2 to CO conversion are known, they are characterized by current densities that are significantly lower than those obtained with solid-state metal materials. Here, we report that a cobalt phthalocyanine bearing a trimethyl ammonium group appended to the phthalocyanine macrocycle is capable of reducing CO2 to CO in water with high activity over a broad pH range from 4 to 14. In a flow cell configuration operating in basic conditions, CO production occurs with excellent selectivity (ca. 95%), and good stability with a maximum partial current density of 165 mA cm-2 (at -0.92 V vs. RHE), matching the most active noble metal-based nanocatalysts. These results represent state-of-the-art performance for electrolytic carbon dioxide reduction by a molecular catalyst.

8.
ChemSusChem ; 12(19): 4500-4505, 2019 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-31432616

RESUMO

A Fe quaterpyridine complex was used as a molecular precursor for the electrochemical reduction of CO2 to CH4 in acetonitrile in the presence of triethanolamine. CH4 was produced with a faradaic yield of approximately 2.1 % at 25 °C and 1 atm pressure of CO2 as reactant. Controlled potential electrolysis coupled to ex situ X-ray photoelectron spectroscopy and X-ray absorption spectroscopy of the electrode surface revealed the formation of metallic iron covered by iron oxides as species responsible for catalysis.

9.
Can J Diet Pract Res ; 79(1): 35-41, 2018 03 01.
Artigo em Inglês | MEDLINE | ID: mdl-28971691

RESUMO

The high prevalence of constipation in long-term care (LTC) residents has been a long-standing issue for caregivers, attending health professionals, and the residents themselves. The traditional medical response has been to utilize pharmaceutical laxatives, enemas, and suppositories for treatment. The purpose of this review was to determine if fibre supplementation (including fibre added to foods) is effective in increasing stool frequency, improving stool consistency, and decreasing laxative use in LTC residents. A systematic search was conducted using PubMed and CINAHL databases, inclusive to March 2017. Search terms included: "long-term care" or "nursing home" AND "fiber (fibre)," "bran," "psyllium," "inulin," or "prebiotic." Intervention trials of fibre supplementation with ≥5 LTC residents were included. The search generated 456 articles following removal of duplicates; 8 studies met the inclusion criteria. Three additional trials were identified through a hand search of references of pertinent articles. Current evidence suggests that added fibre may be effective in increasing stool frequency and/or decreasing laxative use in LTC residents and, thus, may lessen the burden of constipation. However, randomized controlled trials are needed to clearly demonstrate the effects of adding fibre to foods, particularly insoluble and less fermentable sources, on constipation in LTC residents.


Assuntos
Constipação Intestinal/terapia , Fibras na Dieta/administração & dosagem , Laxantes/administração & dosagem , Assistência de Longa Duração/métodos , Canadá/epidemiologia , Constipação Intestinal/epidemiologia , Constipação Intestinal/etiologia , Suplementos Nutricionais , Humanos , Estado Nutricional , Qualidade de Vida
10.
Int Wound J ; 14(3): 546-554, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27488810

RESUMO

Foreign modelling agent reactions (FMAR) are the result of the injection of unapproved high-viscosity fluids with the purpose of cosmetic body modelling. Its consequences lead to ulceration, disfigurement and even death, and it has reached epidemic proportions in several regions of the world. We describe a series of patients treated for FMARs in a specialised wound care centre and a thorough review of the literature. A retrospective chart review was performed from January 1999 to September 2015 of patients who had been injected with non-medical foreign agents and who developed cutaneous ulceration needing treatment at the dermatology wound care centre. This study involved 23 patients whose ages ranged from 22 to 67 years with higher proportion of women and homosexual men. The most commonly injected sites were the buttocks (38·5%), legs (18%), thighs (15·4%) and breasts (11·8%). Mineral oil (39%) and other unknown substances (30·4%) were the most commonly injected. The latency period ranged from 1 week to 17 years. Complications included several skin changes such as sclerosis and ulceration as well as systemic complications. FMAR is a severe syndrome that may lead to deadly complications, and is still very common in Latin America.


Assuntos
Cosméticos/efeitos adversos , Corpos Estranhos/imunologia , Reação a Corpo Estranho/complicações , Óleo Mineral/efeitos adversos , Úlcera Cutânea/etiologia , Úlcera Cutânea/terapia , Adulto , Idoso , Mama/fisiopatologia , Nádegas/fisiopatologia , Cosméticos/administração & dosagem , Feminino , Homossexualidade Masculina/estatística & dados numéricos , Humanos , Masculino , Pessoa de Meia-Idade , Óleo Mineral/administração & dosagem , Estudos Retrospectivos , Pele/fisiopatologia , Adulto Jovem
11.
Case Rep Oncol ; 9(1): 241-5, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27194985

RESUMO

BACKGROUND: B-Raf is a serine/threonine protein kinase activating the MAP kinase/ERK-signaling pathway. It has been shown that 50% of melanomas harbor activating BRAF mutations, with over 90% being the V600E mutation. OBJECTIVE: The goal of this research was to determine the prevalence of the BRAF V600E mutation in patients from Central Mexico diagnosed with primary melanoma. METHODS: Skin biopsies from 47 patients with melanoma were obtained from the dermatology department of the Hospital General 'Dr. Manuel Gea González' in Mexico City. For BRAF mutation determination, after DNA isolation, the gene region where the mutation occurs was amplified by PCR. Subsequently, the presence or absence of the V600E mutation was detected by Sanger sequencing performed at the private molecular diagnostic laboratory Vitagénesis in Monterrey, Mexico. RESULTS: Of the 47 patients sampled, 6.4% harbored the V600E mutation. No statistical significance was found between mutations and the type of tumor.

12.
Dermatol Clin ; 33(2): 273-6, 2015 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-25828717

RESUMO

Nail punch biopsy is used to obtain a tissue sample for the diagnosis and treatment of nail diseases. The best results will be possible if the surgeon is familiar with the anatomy and physiology of the nail apparatus. A punch biopsy can be used in all regions of the nail apparatus in the presence or absence of nail plate. When the procedure is performed with a careful handling of the anatomic site and specimen, in most cases a successful diagnosis can be achieved.


Assuntos
Biópsia/métodos , Doenças da Unha/patologia , Unhas/patologia , Anestesia Local , Biópsia/efeitos adversos , Biópsia/instrumentação , Humanos , Manejo de Espécimes
13.
Dermatol Surg ; 41(3): 411-4, 2015 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-25738445

RESUMO

BACKGROUND: Onychocryptosis is one of the most common painful nail conditions. Conservative treatment may take a long time to obtain effective results. OBJECTIVE: The purpose of this study is to show the effectiveness of a conservative treatment of ingrown nails that shows rapid results. METHODS AND MATERIALS: Patients with painful Stage 1 to 2 onychocryptosis who were not candidates or refused surgery were treated with the cotton cast. The severity and cause of onychocryptosis was clinically evaluated during a 2-month period. A questionnaire was applied to all patients to evaluate pain, final treatment, and possible complications. RESULTS: All patients noticed results in less than 72 hours. Pain subsided in less than 24 hours in half of the patients and before 72 hours in 100% of the patients without the need of other treatments. The use of the cast prevented surgery and the accompanying morbidities in most of the patients (80%). CONCLUSION: The "cotton nail cast" is an effective conservative method for mild nail embedding. It is easy to apply, inexpensive, relieves pain rapidly, and avoids surgery in most patients.


Assuntos
Fibra de Algodão , Cianoacrilatos , Unhas Encravadas/terapia , Dor/prevenção & controle , Idoso , Bandagens , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Unhas Encravadas/complicações , Unhas Encravadas/patologia , Dor/etiologia , Dor/patologia , Paroniquia/etiologia , Paroniquia/patologia , Paroniquia/prevenção & controle , Resultado do Tratamento
14.
Chemistry ; 21(2): 808-13, 2015 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-25370199

RESUMO

The simultaneous measurement of the decrease of available Fe(II) ions and the increase of available Fe(III) ions allowed the analysis of the ferroxidase activity of two distinct apoferritins. Although recombinant human apoferritin (HuFtH) rapidly oxidizes Fe(II) to Fe(III) , this iron is not properly stored in the ferritin cavity, as otherwise occurs in horse-spleen H/L-apoferritin (HsFt; H=heavy subunit, L=light subunit). Iron storage in these apoferritins was also studied in the presence of two copper-loaded mammalian metallothioneins (MT2 and MT3), a scenario that occurs in different brain-cell types. For HuFtH, unstored Fe(III) ions trigger the oxidation of Cu-MT2 with concomitant Cu(I) release. In contrast, there is no reaction with Cu-MT2 in the case of HsFt. Similarly, Cu-MT3 does not react during either HuFtH or HsFt iron reconstitution. Significantly, the combination of ferritin and metallothionein isoforms reported in glia and neuronal cells are precisely those combinations that avoid a harmful release of Fe(II) and Cu(I) ions.


Assuntos
Apoferritinas/metabolismo , Ferritinas/metabolismo , Metalotioneína/metabolismo , Animais , Compostos Férricos/análise , Compostos Férricos/metabolismo , Ferritinas/química , Compostos Ferrosos/análise , Compostos Ferrosos/metabolismo , Cavalos , Humanos , Metalotioneína/análise , Proteínas Recombinantes/metabolismo
15.
Int J Dermatol ; 53(8): 947-51, 2014 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-24527729

RESUMO

BACKGROUND: Progressive hemifacial atrophy or Parry-Romberg Syndrome (PRS) is a rare, acquired, progressive dysplasia of subcutaneous tissue and bone characterized by unilateral facial involvement. Its etiology is unknown, but theories about its pathogenesis include infectious, degenerative, autoimmune, and traumatic causes among others. The causal relationship of PRS and linear morphea en coup de sabre (LMCS) with Borrelia burgdorferi infection remains controversial. Our goal was to serologically determine anti-B. burgdorferi antibodies in patients diagnosed with PRS and LMCS to establish a possible association as a causative agent. METHODS: We conducted a serology study with patients belonging to a group of 21 individuals diagnosed with PRS, six with LMCS, and 21 matched controls. Anti-Borrelia IgG antibodies were determined by ELISA. A descriptive statistical analysis and Fischer's exact test were done. RESULTS: In serological tests, only two cases had borderline values and were further analyzed by Western blot with non-confirmatory results. For both the PRS and LMCS group, the association test was not significant, suggesting a lack of association between PRS or LMCS and the presence of anti-Borrelia antibodies. CONCLUSION: In Mexico there are no previous studies on Borrelia infection and its relationship between PRS or LMCS. Our result showed a lack of association of either clinical entities with anti-Borrelia-antibodies. Former reports of this association may suggest coincidental findings without causal relationship.


Assuntos
Anticorpos Antibacterianos/sangue , Borrelia burgdorferi/imunologia , Hemiatrofia Facial/imunologia , Imunoglobulina G/sangue , Esclerodermia Localizada/imunologia , Adolescente , Adulto , Idoso , Estudos de Casos e Controles , Criança , Hemiatrofia Facial/epidemiologia , Hemiatrofia Facial/microbiologia , Feminino , Humanos , Doença de Lyme/complicações , Masculino , México/epidemiologia , Pessoa de Meia-Idade , Esclerodermia Localizada/epidemiologia , Esclerodermia Localizada/microbiologia , Estudos Soroepidemiológicos , Adulto Jovem
16.
Gac Med Mex ; 150(1): 96-100, 2014.
Artigo em Espanhol | MEDLINE | ID: mdl-24481436

RESUMO

BACKGROUND: Trichoepithelioma is a benign follicular tumor, affects young female adults, and has three clinical forms: solitary, multiple, or desmoplastic. Diagnosis represents a clinical challenge for the dermatologist. There are many differential diagnoses, and the most important is basal cell carcinoma because they share histological features. It has good prognosis and therefore treatment is usually for aesthetic purposes, even though it is associated with recurrence. METHODS: This is a retrospective study carried out from January 1993 to December 2012 of all the patients who were diagnosed by histopathology to have trichoepithelioma in the dermatology department of the Dr. Manuel Gea González General Hospital. RESULTS: There were 47 cases, of which 70.2% were female, with an average age of 43.6 years. Solitary trichoepithelioma was the most frequent clinical form (66%) and only three of 14 cases of multiple trichoepithelioma had a positive family history.


Assuntos
Síndromes Neoplásicas Hereditárias , Neoplasias Cutâneas , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Biópsia , Criança , Feminino , Hospitais Gerais , Humanos , Masculino , Pessoa de Meia-Idade , Síndromes Neoplásicas Hereditárias/patologia , Estudos Retrospectivos , Neoplasias Cutâneas/patologia , Adulto Jovem
17.
Metallomics ; 5(3): 193-6, 2013 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23416900

RESUMO

A silica-protein hybrid material has been prepared by simultaneous covalent deposition of apoferritin and lactoferrin on functionalized silica. This material exhibits strong antibacterial activity against E. coli due to its high iron-uptake capacity.


Assuntos
Antibacterianos/química , Apoferritinas/química , Escherichia coli/efeitos dos fármacos , Ferro/química , Lactoferrina/química , Dióxido de Silício/química , Antibacterianos/farmacologia
18.
J Dermatol Case Rep ; 6(1): 1-4, 2012 Mar 27.
Artigo em Inglês | MEDLINE | ID: mdl-22514581

RESUMO

BACKGROUND: Most patients with oral mucosal melanoma have had a mucosal hyper pigmented area for months or even years before the diagnosis, it is important to consider the differential diagnosis of mucosal melanoma, which in many cases is a difficult diagnosis and because of the aggressive biological behavior of mucosal melanoma it is important to do a quick diagnosis. MAIN OBSERVATION: A 40-year-old Mexican male patient, presented with a lesion on the lower right half of the lip covering almost the entire vermillion border, 1 mm below the white roll. The lesion was a 1.5 x 4 cm pigmented macule with asymmetric and irregular borders and colors. Dermoscopy showed a multi component pattern. An incision biopsy was performed under the impression of mucosal melanoma. The pathologic report described a Clark I vermillion edge mucosal melanoma in situ. CONCLUSIONS: This case had confounding clinical signs that could have misguided the clinician. But dermoscopy proved to be useful when suspecting a malignant lesion, which prompted a biopsy and a correct diagnosis.

19.
PLoS One ; 6(10): e25844, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-22022456

RESUMO

Territoriality is a widespread behaviour in animals and its analysis is crucial in several areas of behavioural, ecological and evolutionary research. Commonly, territory size is assessed through territory mapping and the application of simple area estimators such as minimum convex polygons. In the present study we demonstrate that territory size can be determined adequately with an active approach through intrusion experiments, a technique that is commonly used in behavioural research in other contexts. Tests with simulated data indicate that a minimum of twelve trials needs to be performed to establish reliable orders of relative territory size. To estimate absolute territory size, detailed hull techniques are most appropriate when analyzing point patterns of intrusion experiments, while the local convex hull estimator enables the construction of internal utilization distributions based on such point patterns. Additionally we suggest a 'stretch the centre' approach to emphasize the actual process of intrusion experiments in the construction of internal utilization distributions. To demonstrate the utility of the method, we apply all findings from the simulations to data from fieldwork with the model species Allobates femoralis, a territorial aromobatid frog from the lowland rainforest of French Guiana.


Assuntos
Anuros/fisiologia , Simulação por Computador , Etologia/métodos , Territorialidade , Animais , Feminino , Masculino , Tamanho da Amostra
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