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1.
Nat Prod Res ; : 1-6, 2020 Dec 08.
Artigo em Inglês | MEDLINE | ID: mdl-33289429

RESUMO

Zanthoxylum armatum, its peels possessed better special flavour, as well as various bioactivities, such as anti-inflammatory, anti-microbial and anti-tumour. In our chemical investigation on the peels of Z. armatum, two new lignans (1 and 2) and three known lignans (3-5) were isolated by silica gel column chromatography, ODS column and preparative HPLC and their structures were established as zanthlignans A and B (1-2), (-)-asarinin (3), phylligenin (4) and planispine A (5) through various spectroscopic techniques including UV, IR, HR-ESI-MS, NMR and CD methods.

2.
Neural Plast ; 2020: 8842110, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33299396

RESUMO

Musculoskeletal pain (MSP) is one of the most severe complaints in women undergoing menopause. The prevalence of MSP varied when taking the menopausal state and age factor into consideration. This study investigated the prevalence of MSP in perimenopausal women and its association with menopausal state. The MEDLINE, Embase, Web of Science, and PubMed databases were searched from inception to July 2020, and 16 studies were retrieved for the current meta-analysis. The primary outcome measure was the MSP Odds Ratio (OR). The estimated overall prevalence of MSP among perimenopausal women was 71% (4144 out of 5836, 95% confidence interval (CI): 64%-78%). Perimenopausal women demonstrated a higher risk for MSP than premenopausal ones (OR: 1.63, 95% CI: 1.35-1.96, P = 0.008, I 2 = 59.7%), but similar to that in postmenopausal ones (OR: 1.07, 95% CI: 0.95-1.20, P = 0.316, I 2 = 13.4%). The postmenopausal women were at a higher risk of moderate/severe MSP than the premenopausal ones (OR: 1.45, 95% CI: 1.21-1.75, P = 0.302, I 2 = 16.5%) or the perimenopausal ones (OR: 1.40, 95% CI: 1.09-1.79, P = 0.106, I 2 = 55.4%). In conclusion, the perimenopause is a state during which women are particularly predisposed to develop MSP. As to moderate to severe degrees of MSP, the odds increase linearly with age, from premenopause to peri- and then to postmenopause.

3.
Nat Prod Res ; : 1-6, 2020 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-33118393

RESUMO

Three undescribed prenylated benzoic acid derivatives berberisides A-C (1-3) and a new clerodane glycoside berberiside D (4) were isolated from Berberis tsarica Aherndt. Their structures were elucidated on the basis of extensive NMR and HR-ESI-MS analysis. The in vitro cytotoxic activities of all isolates were studied against lung carcinoma A549, hepatocellular carcinoma HepG2 and breast carcinoma MDA-MB-231 cell lines. Among them, compounds 1 and 4 exhibited anti-proliferative effects against three tumor cell lines with IC50 ranging from 28.97 ± 2.18 to 35.83 ± 0.72 µM.

5.
J Agric Food Chem ; 68(19): 5327-5338, 2020 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-32320608

RESUMO

Panax notoginseng inflorescences (PNI) and leaves (PNL) are commonly used as folk medicine and food supplements. In this study, an online two-dimensional hydrophilic interaction × reversed-phase liquid chromatography coupled to linear trap quadropole mass spectrometry method was developed to determine 24 ginsenosides, including two novel compounds, in PNI and PNL extracted by water and methanol. Our data demonstrated that ginsenosides Rd, Rc, Rb2, Rb3, Rb1, Ra2, Ra1, and Ra3 in both PNI and PNL extracted by water rather than methanol can be transformed to ginsenoside F2, notoginsenoside Fe, ginsenoside Rd2, notoginsenoside Fd, gypenoside XVII, PN02, PN01, and PN03, respectively, by selectively cleaving the ß-(1→2)-glucosidic linkage at the C-3 position. Ginsenoside transformation was further verified to be mediated by the proteins isolated from samples. Additionally, the two newly discovered transformed products, namely, PN02 and PN03, were prepared and identified as novel compounds by nuclear magnetic resonance. Our findings provide new insight into the importance of extraction solvents on the component profile of natural products.

6.
Nat Prod Res ; 34(8): 1124-1130, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30873860

RESUMO

One new neolignan glycoside, dolomiside A (1), together with 11 known phenylpropanoid glycosides were isolated from Dolomiaea souliei (Franch.) Shih. The structures of these isolates were determined by UV, CD, HR-ESI-TOFMS, 1D and 2D NMR analysis.


Assuntos
Asteraceae/química , Glicosídeos/isolamento & purificação , Lignanas/isolamento & purificação , Glicosídeos Cardíacos , Medicamentos de Ervas Chinesas/química , Glicosídeos/química , Lignanas/química , Estrutura Molecular , Análise Espectral
7.
Int J Biol Macromol ; 136: 341-351, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31202844

RESUMO

A neutral polysaccharide (ALP-1) and an acidic polysaccharide (ALP-3) were purified from Atractylodes lancea (Thunb.) DC. ALP-1 exhibited a linear backbone composed of (2 → 1)-linked ß-d-fructofuranose. The backbone of ALP-3 was elucidated as →4)-GalAp-(1 → 3,4)-Rhap-(1→, and with branch chain substituted at O-3 position of →3,4)-Rhap-(1→. The branch chain consists of →3,5)-Araf-(1→, →5)-Araf-(1→, and Araf-(1→. Particularly, ALP-3 could significantly stimulate macrophage proliferation in a dose-dependent manner, and stimulate phagocytic, NO and cytokines production than ALP-1 on RAW264.7 cells. Besides, both ALP-1 and ALP-3 could activate T-cells in Peyer's patch cells and enhance the production of colony stimulation factors. While the ALP-3 also showed better intestinal immune system modulating activity than ALP-1. The result of this study indicated that the structure diversity of polysaccharide is crucial for its bioactivity. And also provided evidence for that carbohydrate polymers in A. lancea definitely contributed to its pharmaceutical effects.


Assuntos
Atractylodes/química , Fatores Imunológicos/química , Fatores Imunológicos/farmacologia , Polissacarídeos/química , Polissacarídeos/farmacologia , Rizoma/química , Animais , Sequência de Carboidratos , Citocinas/metabolismo , Camundongos , Óxido Nítrico/biossíntese , Fagocitose/efeitos dos fármacos , Células RAW 264.7 , Relação Estrutura-Atividade
8.
Mar Drugs ; 16(9)2018 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-30208577

RESUMO

Seven long-chain amides, including five previously undescribed bacillamidins A⁻E (1⁻5) and two previously reported synthetic analogs, bacillamidins F (6) and G (7), were isolated from extracts of the marine-derived Bacillus pumilus strain RJA1515. The structures of the new compounds were established by extensive analysis of 1D and 2D nuclear magnetic resonance (NMR) data as well as high resolution mass spectrometry (HRMS), and the absolute configurations of the stereogenic carbons of 1⁻4 were established by comparison of the calculated and the experimental electronic circular dichroism (ECD) spectra. The cytotoxic and antimicrobial activities of 1⁻7 were evaluated.


Assuntos
Amidas/farmacologia , Anti-Infecciosos/farmacologia , Antineoplásicos/farmacologia , Organismos Aquáticos/química , Bacillus pumilus/química , Amidas/química , Amidas/isolamento & purificação , Anti-Infecciosos/química , Anti-Infecciosos/isolamento & purificação , Antineoplásicos/química , Antineoplásicos/isolamento & purificação , Bactérias/efeitos dos fármacos , Linhagem Celular Tumoral , Dicroísmo Circular , Humanos , Espectroscopia de Ressonância Magnética , Testes de Sensibilidade Microbiana , Estrutura Molecular
9.
Chin Med ; 13: 13, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29541156

RESUMO

Background: Coptidis rhizoma (CR) is the dried rhizome of Coptis chinensis Franch., C. deltoidea C. Y. Cheng et Hsiao or C. teeta Wall. (Ranunculaceae) and is commonly used in Traditional Chinese Medicine for the treatment of various diseases including bacillary dysentery, typhoid, tuberculosis, epidemic cerebrospinal meningitis, empyrosis, pertussis, and other illnesses. Methods: A literature survey was conducted via SciFinder, ScieneDirect, PubMed, Springer, and Wiley databases. A total of 139 selected references were classified on the basis of their research scopes, including chemical investigation, quality evaluation and pharmacological studies. Results: Many types of secondary metabolites including alkaloids, lignans, phenylpropanoids, flavonoids, phenolic compounds, saccharides, and steroids have been isolated from CR. Among them, protoberberine-type alkaloids, such as berberine, palmatine, coptisine, epiberberine, jatrorrhizine, columamine, are the main components of CR. Quantitative determination of these alkaloids is a very important aspect in the quality evaluation of CR. In recent years, with the advances in isolation and detection technologies, many new instruments and methods have been developed for the quantitative and qualitative analysis of the main alkaloids from CR. The quality control of CR has provided safety for pharmacological applications. These quality evaluation methods are also frequently employed to screen the active components from CR. Various investigations have shown that CR and its main alkaloids exhibited many powerful pharmacological effects including anti-inflammatory, anti-cancer, anti-diabetic, neuroprotective, cardioprotective, hypoglycemic, anti-Alzheimer and hepatoprotective activities. Conclusion: This review summarizes the recent phytochemical investigations, quality evaluation methods, the biological studies focusing on CR as well as its main alkaloids.

10.
Molecules ; 22(12)2017 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-29186881

RESUMO

Panacis Japonici Rhizoma (Zhu-Jie-Shen in Chinese), the root of P. japonicus C.A. Mey., is commonly used in traditional Chinese Medicine. Saponins are the major bioactive compounds in this herb. The similarity of polarity and structure of the natural products in herb caused the difficulty of purification and resulted in the shortage and high cost of the reference compounds, which has greatly hindered efforts toward quantification in quality control. A novel strategy using a standardized reference fraction for qualification of the major saponins in Panacis Japonici Rhizoma was proposed to easily and effectively control the quality of PJR. The strategy is feasible and reliable, and the methodology of the developed approach is also validated. The standardized reference fraction was used for quantification, which might solve the shortage of the pure reference compounds in the quality control of herbal medicines.


Assuntos
Ginsenosídeos/química , Extratos Vegetais/química , Raízes de Plantas/química , Rizoma/química , Saponinas/química , Cromatografia Líquida de Alta Pressão/métodos , Ginsenosídeos/isolamento & purificação , Limite de Detecção , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/normas , Plantas Medicinais/química , Controle de Qualidade , Padrões de Referência , Reprodutibilidade dos Testes , Saponinas/isolamento & purificação
11.
Phytochemistry ; 142: 76-84, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28688991

RESUMO

Five previously undescribed compounds including two triterpenoid aglycones, 3ß,23-dihydroxy-1,12-dioxo-olean-28-oic acid and 3ß,23,27-trihydroxy-1-oxo-olean-12-ene-28-oic acid, and three triterpenoid glucosides cyclocarioside L-N, along with 17 known compounds were isolated from a CH3Cl-soluble extract of the leaves of Cyclocarya paliurus. Two 27-nor-triterpenoid glycosides were isolated from the genus for the first time. Furthermore, the characterized compounds were tested for the inhibitory effects on apoliprotein B48 secretion in Caco-2 cells. Seven triterpenoid aglycones together with four triterpenoid saponins significantly decreased the apoliprotein B48 oversecretion induced by oleic acid in Caco-2 cells.


Assuntos
Apolipoproteína B-48/antagonistas & inibidores , Medicamentos de Ervas Chinesas/isolamento & purificação , Medicamentos de Ervas Chinesas/farmacologia , Juglandaceae/química , Triterpenos/isolamento & purificação , Triterpenos/farmacologia , Células CACO-2 , Medicamentos de Ervas Chinesas/química , Glicosídeos/química , Glicosídeos/isolamento & purificação , Humanos , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Ácido Oleico/farmacologia , Folhas de Planta/efeitos dos fármacos , Saponinas/química , Saponinas/isolamento & purificação , Saponinas/farmacologia , Triterpenos/química
13.
Phytomedicine ; 23(13): 1555-1565, 2016 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-27823619

RESUMO

BACKGROUND: Panacis Japonici Rhizoma (PJR) is one of the most famous Chinese medical herbs that is known for exhibiting potential anti-cancer effects. PURPOSE: This study aims to isolate and investigate the anti-cancer potential of saponins from PJR in ovarian cancer cells. METHODS: The compounds were separated by comprehensive chromatographic methods. By comparison of the 1H- and 13C NMR data, as well as the HR-ESI-MS data, with the corresponding references, the structures of compounds were determined. MTT assay was performed to evaluate cell viability, along with flow cytometry for cell cycle analysis. JC-1 staining, Annexin V-PI double staining as well as Hoechst 33; 342 staining were used for detecting cell apoptosis. Western blot analysis was conducted to determine the relative protein level. Transwell assays were performed to investigate the effect of the saponin on cell migration and invasion and zymography experiments were used to detect the enzymatic activities. RESULTS: Eleven saponins were isolated from PJR and their anti-proliferative effects were evaluated in human ovarian cancer cells. Chikusetsusaponin IVa methyl ester (1) exhibited the highest anti-proliferative potential among these isolates with the IC50 values at less than 10 µM in both ovarian cancer A2780 and HEY cell lines. Compound 1 induced G1 cell cycle arrest accompanied with an S phase decrease, and down-regulated the expression of cyclin D1, CDK2, and CDK6. Further study showed that compound 1 effectively decreased the cell mitochondrial membrane potential, increased the annexin V positive cells and nuclear chromatin condensation, as well as enhanced the expression of cleaved PARP, Bax and cleaved-caspase 3 while decreasing that of Bcl-2. Moreover, compound 1 suppressed the migration and invasion of HEY and A2780 cells, down-regulated the expression of Cdc42, Rac, RohA, MMP2 and MMP9, and decreased the enzymatic activities of MMP2 and MMP9. CONCLUSION: These results provide a comprehensive evaluation of compound 1 as a potential agent for the treatment of ovarian cancer.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Ácido Oleanólico/análogos & derivados , Neoplasias Ovarianas/tratamento farmacológico , Saponinas/farmacologia , Antineoplásicos Fitogênicos/química , Apoptose/efeitos dos fármacos , Caspase 3/metabolismo , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Ciclina D1/metabolismo , Quinase 2 Dependente de Ciclina/metabolismo , Ensaios de Seleção de Medicamentos Antitumorais , Medicamentos de Ervas Chinesas/química , Feminino , Pontos de Checagem da Fase G1 do Ciclo Celular/efeitos dos fármacos , Humanos , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Estrutura Molecular , Ácido Oleanólico/química , Ácido Oleanólico/farmacologia , Neoplasias Ovarianas/metabolismo , Neoplasias Ovarianas/patologia , Saponinas/química
14.
Neural Plast ; 2016: 2130901, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26819761

RESUMO

To mimic multilevel nerve root compression and intervertebral foramina stenosis in human, we established a new animal model of the chronic compression of unilateral multiple lumbar DRGs (mCCD) in the rat. A higher occurrence of signs of spontaneous pain behaviors, such as wet-dog shaking and spontaneous hind paw shrinking behaviors, was firstly observed from day 1 onward. In the meantime, the unilateral mCCD rat exhibited significant bilateral hind paw mechanical and cold allodynia and hyperalgesia, as well as a thermal preference to 30°C plate between 30 and 35°C. The expression of activating transcription factor 3 (ATF3) was significantly increased in the ipsilateral and contralateral all-sized DRG neurons after the mCCD. And the expression of CGRP was significantly increased in the ipsilateral and contralateral large- and medium-sized DRG neurons. ATF3 and CGRP expressions correlated to evoked pain hypersensitivities such as mechanical and cold allodynia on postoperative day 1. The results suggested that bilateral neuropathy of primary sensory neurons might contribute to bilateral hypersensitivity in the mCCD rat.


Assuntos
Gânglios Espinais/fisiopatologia , Hiperalgesia/fisiopatologia , Síndromes de Compressão Nervosa/fisiopatologia , Doenças do Sistema Nervoso Periférico/fisiopatologia , Animais , Comportamento Animal/fisiologia , Modelos Animais de Doenças , Hiperalgesia/etiologia , Masculino , Síndromes de Compressão Nervosa/complicações , Medição da Dor , Doenças do Sistema Nervoso Periférico/etiologia , Ratos , Ratos Sprague-Dawley
15.
Guang Pu Xue Yu Guang Pu Fen Xi ; 36(4): 991-5, 2016 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-30048095

RESUMO

Peroxpolytungstic acid (PPTA) is one of the important precursors to synthesize nanostructured tungsten oxides with the chemical routes. In this paper,PPTA sol, being prepared with H2O2, W and C2H5OH, was placed at room temperature for a long time till it was jellified naturally and then was dried at 120 ℃ for 3 hours for this investigation. Structures, thermal stability and optical UV-Vis absorption of the samples have been investigated by XRD, SEM, Raman, TG/DSC and UV-Vis spectrum, respectively. The wide peaks from XRD indicate that the sample is amorphous. The Gaussian fitting for these XRD peaks indicates that the sample is the composite of tungsten oxides and tungsten oxide hydrates. SEM indicates that the sample possesses the morphologies of the nano-particles with the sizes of about 50~100 nm and the nano-flakes with the thickness of about 50 nm. The Gaussian fitting of the wide Raman peaks illustrates that the sample possesses the obvious the modes of symmetrical O­W­O stretching, asymmetrical O­W­O stretching and WO vibration accompanied with the modes of symmetrical O­W­O bending, asymmetrical O­W­O bending and the adsorptive water vibration. This fact further indicates that the sample is composed of the amorphous tungsten oxides and tungsten oxide hydrates. Analysis on the TG/DSC curves indicates that PPTA gels possess four different thermal dynamic processes with increasing the treatment temperature from 120 to 500 ℃: (1) the slow crystallization of PPTA (120~165 ℃), (2) the dissociation of adsorptive H2O2 and the desorption of adsorptive H2O (165~236 ℃), (3) the quick decomposition of tungsten oxide hydrate (236~287 ℃) and (4) the crystallization and phase transformation of the final products WO3 (287~500 ℃). Optical absorption of PPTA gels happens in the range of 350~600 nm, where the intensity of the optical absorption gradually increases and finally reaches the saturation with the increase of photon energy. The optical band gap was estimated to be about 2.25 eV, being obviously lower than the known values for WO3 and H2WO4 (2.48~3.50 eV). The key factors for the low gap value of the composite can be attributed to the molecular water, the oxygen defects and the structural distortion.

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