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1.
Hepatol Int ; 15(6): 1431-1441, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34843069

RESUMO

BACKGROUND: Mesenchymal stem cell (MSC) infusion was reported to improve liver function in patients with decompensated liver cirrhosis (DLC); however, whether the medication can improve outcome of these patients is poorly understood. METHODS: This prospective, open-labeled, randomized controlled study enrolled 219 patients with HBV-related DLC who were divided into control group (n = 111) and umbilical cord-derived MSC (UC-MSC)-treated group (n = 108), then all of them received a follow-up check from October 2010 to October 2017. The treated patients received three times of UC-MSC infusions at 4-week intervals plus conventional treatment that was only used for control group. The overall survival rate and HCC-free survival rate were calculated as primary endpoints and the liver function and adverse events associated with the medication were also evaluated. RESULTS: During the follow-up check period from 13 to 75th months, there was a significantly higher overall survival rate in the treated group than the control group, while the difference of the hepatocellular carcinoma event-free survival rate between the treated and control groups was not observed during the 75-month follow-up. UC-MSC treatment markedly improved liver function, as indicated by the levels of serum albumin, prothrombin activity, cholinesterase, and total bilirubin during 48 weeks of follow-up. No significant side effects or treatment-related complications were observed in the UC-MSC group. CONCLUSIONS: Therapy of UC-MSC is not only well tolerated, but also significantly improves long-term survival rate, as well as the liver function in patients with HBV-related DLC. UC-MSC medication, therefore, might present a novel therapeutic approach for the disease.

2.
Front Pharmacol ; 12: 704481, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34483908

RESUMO

Background: Psoriasis is a T help 17 (Th17) cell-mediated chronic inflammatory skin disease. Recent studies have shown that dihydroartemisinin (DHA) can significantly reduce experimental autoimmune encephalomyelitis and rheumatoid arthritis by regulating Th17 cells. Objective: To verify whether DHA can improve the symptoms of psoriasis and to further explore the possible mechanism. Methods: The efficiency of DHA was preliminary detected on human keratinocytes (HaCaT) cells in psoriatic condition. Then, imiquimod-induced psoriasis-like model in BALB/c mice was established to evaluate the effects of DHA in vivo. Results: Under the stimulation of tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ), DHA inhibited the proliferation of HaCaT cells and significantly affected the mRNA expression levels of IFN-γ, interleukin (IL), IL-17A and IL-23. DHA treatment reduced the severity of psoriasis-like skin and resulted in less infiltration of immune cells in skin lesions. DHA restored the expression of IFN-γ, IL-17A, and IL-23 in skins, as well as a decrease of cytokines and chemokines in skin supernatant. DHA also altered the cellular composition in the spleen, which is the makeup of the T cells, dendritic cells (DCs), and macrophages. DHA recovered Th17-related profile with decreased frequency of IL-17+CD4+T cells from splenocyte of mice. Furthermore, DHA also inhibited the concentration of IL-17 from Th17 cells and the expression of Th17 cell-related transcription factors retinoid-related orphan receptor-gamma t (ROR-γt) in vitro. In addition, phosphorylation of signal transducer and activator of transcription-3 (STAT3) was significantly reduced in DHA treatment mice, suggesting that the IL-23/Th17 axis plays a pivotal role. Conclusion: DHA inhibits the progression of psoriasis by regulating IL-23/Th17 axis and is expected to be an effective drug for the treatment of psoriasis.

3.
Nat Metab ; 3(7): 909-922, 2021 07.
Artigo em Inglês | MEDLINE | ID: mdl-34158670

RESUMO

Exosomes represent a subtype of extracellular vesicle that is released through retrograde transport and fusion of multivesicular bodies with the plasma membrane1. Although no perfect methodologies currently exist for the high-throughput, unbiased isolation of pure plasma exosomes2,3, investigation of exosome-enriched plasma fractions of extracellular vesicles can confer a glimpse into the endocytic pathway on a systems level. Here we conduct high-coverage lipidomics with an emphasis on sterols and oxysterols, and proteomic analyses of exosome-enriched extracellular vesicles (EVs hereafter) from patients at different temporal stages of COVID-19, including the presymptomatic, hyperinflammatory, resolution and convalescent phases. Our study highlights dysregulated raft lipid metabolism that underlies changes in EV lipid membrane anisotropy that alter the exosomal localization of presenilin-1 (PS-1) in the hyperinflammatory phase. We also show in vitro that EVs from different temporal phases trigger distinct metabolic and transcriptional responses in recipient cells, including in alveolar epithelial cells, which denote the primary site of infection, and liver hepatocytes, which represent a distal secondary site. In comparison to the hyperinflammatory phase, EVs from the resolution phase induce opposing effects on eukaryotic translation and Notch signalling. Our results provide insights into cellular lipid metabolism and inter-tissue crosstalk at different stages of COVID-19 and are a resource to increase our understanding of metabolic dysregulation in COVID-19.


Assuntos
COVID-19/metabolismo , COVID-19/virologia , Vesículas Extracelulares/metabolismo , Lipidômica , Metabolômica , SARS-CoV-2 , Transporte Biológico , COVID-19/epidemiologia , Fracionamento Celular , Membrana Celular/metabolismo , Fracionamento Químico , Análise por Conglomerados , Biologia Computacional/métodos , Exossomos/metabolismo , Interações Hospedeiro-Patógeno , Humanos , Lipidômica/métodos , Metaboloma , Metabolômica/métodos , Estudos Retrospectivos , SARS-CoV-2/genética , SARS-CoV-2/imunologia
4.
Infect Dis Poverty ; 9(1): 161, 2020 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-33239109

RESUMO

BACKGROUND: Coronavirus disease 2019 (COVID-19) is pandemic. It is critical to identify COVID-19 patients who are most likely to develop a severe disease. This study was designed to determine the clinical and epidemiological features of COVID-19 patients associated with the development of pneumonia and factors associated with disease progression. METHODS: Seventy consecutive patients with etiologically confirmed COVID-19 admitted to PLA General Hospital in Beijing, China from December 27, 2019 to March 12, 2020 were enrolled in this study and followed-up to March 16, 2020. Differences in clinical and laboratory findings between COVID-19 patients with pneumonia and those without were determined by the χ2 test or the Fisher exact test (categorical variables) and independent group t test or Mann-Whitney U test (continuous variables). The Cox proportional hazard model and Generalized Estimating Equations were applied to evaluate factors that predicted the progression of COVID-19. RESULTS: The mean incubation was 8.67 (95% confidence interval, 6.78-10.56) days. Mean duration from the first test severe acute respiratory syndrome coronavirus 2-positive to conversion was 11.38 (9.86-12.90) days. Compared to pneumonia-free patients, pneumonia patients were 16.5 years older and had higher frequencies of having hypertension, fever, and cough and higher circulating levels of neutrophil proportion, interleukin-6, low count (< 190/µl) of CD8+ T cells, and neutrophil/lymphocyte ratio. Thirteen patients deteriorated during hospitalization. Cox regression analysis indicated that older age and higher serum levels of interleukin-6, C-reactive protein, procalcitonin, and lactate at admission significantly predicted the progression of COVID-19. During hospitalization, circulating counts of T lymphocytes, CD4+ T cells, and CD8+ T cells were lower, whereas neutrophil proportion, neutrophil/lymphocyte ratio, and the circulating levels of interleukin-6, C-reactive protein, and procalcitonin were higher, in pneumonia patients than in pneumonia-free patients. CD8+ lymphocyte count in pneumonia patients did not recover when discharged. CONCLUSIONS: Older age and higher levels of C-reactive protein, procalcitionin, interleukin-6, and lactate might predict COVID-19 progression. T lymphocyte, especially CD8+ cell-mediated immunity is critical in recovery of COVID-19. This study may help in predicting disease progression and designing immunotherapy for COVID-19.


Assuntos
Linfócitos T CD8-Positivos/patologia , COVID-19/patologia , Interleucina-6/sangue , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , COVID-19/diagnóstico , COVID-19/epidemiologia , Criança , Pré-Escolar , China/epidemiologia , Progressão da Doença , Feminino , Hospitalização , Humanos , Contagem de Leucócitos , Masculino , Pessoa de Meia-Idade , Neutrófilos/patologia , Pneumonia Viral/diagnóstico , Pneumonia Viral/epidemiologia , Pneumonia Viral/patologia , Fatores de Risco , SARS-CoV-2 , Adulto Jovem
5.
Inquiry ; 57: 46958020948668, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32783479

RESUMO

Resistiveness to care is very common among patients of dementia as these patients do not take medicines, meals or bath very easily. Indeed, it is a very challenging task for health caregivers and there is a significant rise in time and cost involved in managing dementia patients. Amongst different factors, the type of communication between resident dementia patients and health caregivers is an important contributing factor in the development of resistiveness to care. Elderspeak (baby talk) is a type of communication in which health caregivers adjust their language and style while interacting with elderly and dependent patients. It involves the use of short sentences, simple grammar, slow and high pitch voice, repeating phrases to provide a comfortable and friendly environment to patients. Most of the time, caregivers tend to adapt elderspeak as they handle weak and fragile older patients for routine activities. Although elderspeak is meant to provide support, warmth and care to patients, yet patients perceive elderspeak as patronizing and it induces negative feelings about self-esteem. Scientists have found a correlation between the development of resistiveness to care and the extent of elderspeak in communication. Therefore, there have been strategies to develop alternative communication strategies by avoiding the use of elderspeak. Moreover, the beneficial effects of such communications have been documented as it improves the quality of life, reduces aggression, agitation and psychosocial symptoms. The present review discusses the scientific studies discussing the use of elderspeak in communication and development of resistiveness to care in resident patients of dementia.


Assuntos
Demência , Qualidade de Vida , Idoso , Cuidadores , Comunicação , Demência/terapia , Humanos , Relações Enfermeiro-Paciente
6.
Nat Commun ; 11(1): 3410, 2020 07 08.
Artigo em Inglês | MEDLINE | ID: mdl-32641700

RESUMO

COVID-19 is associated with 5.1% mortality. Although the virological, epidemiological, clinical, and management outcome features of COVID-19 patients have been defined rapidly, the inflammatory and immune profiles require definition as they influence pathogenesis and clinical expression of COVID-19. Here we show lymphopenia, selective loss of CD4+ T cells, CD8+ T cells and NK cells, excessive T-cell activation and high expression of T-cell inhibitory molecules are more prominent in severe cases than in those with mild disease. CD8+ T cells in patients with severe disease express high levels of cytotoxic molecules. Histochemical studies of lung tissue from one fatality show sub-anatomical distributions of SARS-CoV-2 RNA and massive infiltration of T cells and macrophages. Thus, aberrant activation and dysregulation of CD8+ T cells occur in patients with severe COVID-19 disease, an effect that might be for pathogenesis of SARS-CoV-2 infection and indicate that immune-based targets for therapeutic interventions constitute a promising treatment for severe COVID-19 patients.


Assuntos
Betacoronavirus/patogenicidade , Infecções por Coronavirus/imunologia , Infecções por Coronavirus/patologia , Pneumonia Viral/imunologia , Pneumonia Viral/patologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Betacoronavirus/imunologia , Biomarcadores/metabolismo , Linfócitos T CD4-Positivos/imunologia , Linfócitos T CD4-Positivos/patologia , Linfócitos T CD8-Positivos/imunologia , Linfócitos T CD8-Positivos/patologia , COVID-19 , Quimiotaxia de Leucócito , China/epidemiologia , Infecções por Coronavirus/sangue , Infecções por Coronavirus/virologia , Citocinas/sangue , Feminino , Humanos , Inflamação , Células Matadoras Naturais/imunologia , Células Matadoras Naturais/patologia , Contagem de Leucócitos , Pulmão/imunologia , Pulmão/virologia , Ativação Linfocitária , Masculino , Pessoa de Meia-Idade , Pandemias , Pneumonia Viral/sangue , Pneumonia Viral/virologia , SARS-CoV-2
7.
Cell Metab ; 32(2): 188-202.e5, 2020 08 04.
Artigo em Inglês | MEDLINE | ID: mdl-32610096

RESUMO

The coronavirus disease 2019 (COVID-19) pandemic presents an unprecedented threat to global public health. Herein, we utilized a combination of targeted and untargeted tandem mass spectrometry to analyze the plasma lipidome and metabolome in mild, moderate, and severe COVID-19 patients and healthy controls. A panel of 10 plasma metabolites effectively distinguished COVID-19 patients from healthy controls (AUC = 0.975). Plasma lipidome of COVID-19 resembled that of monosialodihexosyl ganglioside (GM3)-enriched exosomes, with enhanced levels of sphingomyelins (SMs) and GM3s, and reduced diacylglycerols (DAGs). Systems evaluation of metabolic dysregulation in COVID-19 was performed using multiscale embedded differential correlation network analyses. Using exosomes isolated from the same cohort, we demonstrated that exosomes of COVID-19 patients with elevating disease severity were increasingly enriched in GM3s. Our work suggests that GM3-enriched exosomes may partake in pathological processes related to COVID-19 pathogenesis and presents the largest repository on the plasma lipidome and metabolome distinct to COVID-19.


Assuntos
Infecções por Coronavirus/sangue , Infecções por Coronavirus/patologia , Exossomos/metabolismo , Gangliosídeo G(M3)/sangue , Gangliosídeos/sangue , Pneumonia Viral/sangue , Pneumonia Viral/patologia , Adulto , Idoso , Betacoronavirus , Linfócitos T CD4-Positivos/imunologia , Linfócitos T CD4-Positivos/patologia , COVID-19 , Diglicerídeos/sangue , Feminino , Humanos , Masculino , Metaboloma/fisiologia , Metabolômica/métodos , Pessoa de Meia-Idade , Pandemias , SARS-CoV-2 , Esfingomielinas/sangue , Espectrometria de Massas em Tandem , Adulto Jovem
8.
Artif Cells Nanomed Biotechnol ; 47(1): 757-766, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30856346

RESUMO

Inflammatory bowel disease (IBD) is an intestinal chronic inflammatory disease, and is related to imbalance of CD4+T subsets. However, the current treatments of chronic colitis are not ideal and have potential side effects. Therefore, more effective and safer biologically active substances which are extracted from natural plants have been widely concerned. In this study, it was found that Inonotus obliquus polysaccharides (IOP), the main bioactive constituent of Inonotus obliquus, can alleviate dextran sodium sulfate-induced chronic murine intestinal inflammation. Oral administration of IOP (100, 200, 300 mg/kg) can significantly reduce the disease active index and alleviate the pathological changes in colitis mice, where the tight junction proteins Occludin and ZO-1 losses in colon tissues were reduced. It can also regulate imbalanced Th1/Th2 and Th17/Treg in colon tissues, mesenteric lymph nodes and spleen using Reverse Transcription-Polymerase Chain Reaction detection and flow cytometry. Immunohistochemistry and western blot assays further revealed the modulatory effect of IOP on the p-STAT1, p-STAT6, p-STAT3 expression, which promoted the balance of Th1/Th2, Th17/Treg in the colon of chronic colitis mice. In short, these results indicated that IOP was potentially effective therapeutic agent for IBD.


Assuntos
Basidiomycota/química , Colite/tratamento farmacológico , Polissacarídeos Fúngicos/farmacologia , Linfócitos T Reguladores/patologia , Equilíbrio Th1-Th2/efeitos dos fármacos , Células Th17/patologia , Animais , Colite/induzido quimicamente , Colite/patologia , Colo/efeitos dos fármacos , Colo/imunologia , Colo/metabolismo , Colo/patologia , Citocinas/metabolismo , Sulfato de Dextrana/toxicidade , Modelos Animais de Doenças , Doenças Inflamatórias Intestinais/induzido quimicamente , Doenças Inflamatórias Intestinais/tratamento farmacológico , Doenças Inflamatórias Intestinais/patologia , Contagem de Linfócitos , Masculino , Camundongos Endogâmicos BALB C , Fatores de Transcrição STAT/metabolismo , Transdução de Sinais/efeitos dos fármacos , Baço/imunologia
9.
Hepatol Res ; 48(2): 153-164, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-28456135

RESUMO

AIM: To determine the differential characteristics and prognosis of patients with hepatitis B virus (HBV)-related acute-on-chronic liver failure (ACLF) detected using Asian Pacific Association for the Study of the Liver (APASL) criteria and then classified using European Association for the Study of the Liver-Chronic Liver Failure (EASL-CLIF) criteria. METHODS: We retrospectively reviewed 316 HBV-related APASL ACLF patients treated at Beijing 302 Hospital or Beijing You'An Hospital (both Beijing, China) between February 2015 and February 2016. Clinical characteristics and mortality rates were compared among patients with different EASL-CLIF ACLF severity grades (no ACLF, and ACLF grades 1-3). RESULTS: According to the EASL-CLIF criteria, 138 patients had no ACLF, 123 had ACLF at enrollment, and 55 developed ACLF during hospitalization. Both 28-day and 90-day transplant-free mortality were dramatically lower in patients without EASL-CLIF ACLF (0.7% and 5.1%, respectively) than in patients with EASL-CLIF ACLF (40.7% and 63.2%, respectively; both P < 0.001). Liver failure rates were similar in patients with and without EASL-CLIF ACLF, but extrahepatic organ failure was rare in patients without EASL-CLIF ACLF. Baseline serum creatinine, new bacterial infection and new acute kidney injury during hospitalization, maximum rising rates of CLIF-C ACLF score, and Model for End-stage Liver Disease score were independent predictors of EASL-CLIF ACLF after enrollment. CONCLUSIONS: The EASL-CLIF ACLF classification can accurately prognosticate the short-term mortality of patients with HBV-related APASL ACLF. It can also distinguish distinct clinical characteristics and prognoses in patients with and without EASL-CLIF ACLF.

10.
Huan Jing Ke Xue ; 38(9): 3635-3644, 2017 Sep 08.
Artigo em Chinês | MEDLINE | ID: mdl-29965242

RESUMO

Concentrations of seven heavy metals in the coastal sediments of the northern Dongying City were measured. The spatial distribution and sources of heavy metals were discussed and the ecological risk was assessed using sediment quality guidelines (SQGs) and the potential ecological risk index (PERI). The concentration ranges of Hg, As, Pb, Cd, Cr, Cu, and Zn were calculated as 0.005-0.092, 3.44-10.41, 6.59-19.00, 0.50-1.10, 32.42-60.25, 1.72-23.78, and 31.13-69.96 mg·kg-1, respectively. Higher metal concentrations were observed at site S10, which was close to the mouth of Tiaohe River and contained plenty of organic matter, silt, and clay. Principal component analysis (PCA) indicated that As and Pb in the sediments were derived from natural weathering processes, while other metals were mainly attributed to anthropogenic sources, i.e., land-sourced pollutants transported by runoff. Concentrations of As, Cd, Cr, and Cu at some sampling sites exceeded the threshold effect level (TEL) stated in the SQGs implying occasional harmful effects on biological life. PERI showed that the sediments in this area generally had a medium risk, except sites S10, S3, and S9 posing a considerable risk, and that Cd and Hg were the major contributors to the ecological risk. It is necessary to take effective measures to control heavy metal fluxes from rivers around this area and to reduce the risk.


Assuntos
Monitoramento Ambiental , Sedimentos Geológicos/análise , Metais Pesados/análise , Poluentes Químicos da Água/análise , China , Ecologia , Metais Pesados/efeitos adversos , Medição de Risco , Rios , Poluentes Químicos da Água/efeitos adversos
11.
J Gastroenterol Hepatol ; 29(3): 554-60, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-24117714

RESUMO

BACKGROUND AND AIM: Liver transplantation (LT) for hepatitis B virus (HBV)-related disease can be complicated by HBV recurrence. The aim of this study was to evaluate the risk factors, prophylaxis treatment, and histological characteristics of HBV recurrence after LT when using long-term, low-dose hepatitis B immunoglobulin (HBIG) plus nucleoside analog (lamivudine [LAM] or entecavir [ETV]). METHODS: Retrospective data from 253 adult LT patients using long-term, low-dose HBIG plus nucleoside analog after LT, for a mean treatment duration of 1-72 months, were collected from a single center in Beijing, China. Univariate analyses were conducted to determine the association among gender, age, hepatocellular carcinoma, hepatitis B e antigen-positive status, HBV-DNA level and tyrosine-methionine-aspartate-aspartate (YMDD) mutations on HBV recurrence in these patients. RESULTS: Overall, the HBV recurrence rate was 6.32% (16/253). There was no significant difference in the survival rate between the HBV recurrence and non-recurrence groups. Risk factors for HBV recurrence were: hepatitis B e antigen positivity, HBV-DNA > 10(5) copies/mL, hepatocellular carcinoma, and YMDD mutation. Sixteen patients receiving LAM had HBV recurrence (16/169; mean treatment duration: 61.8 ± 18.3 months). No HBV recurrence occurred in patients receiving ETV after LT (0/84; mean treatment duration: 57.1 ± 15.9 months). Differences in rate of mortality and HBV recurrence were not significant between the two groups. CONCLUSIONS: LT is an effective treatment for HBV-related end-stage liver disease. The combination of ETV and intramuscular HBIG for HBV recurrence prophylaxis after LT was more effective than LAM, especially in Chinese patients with HBV recurrence risk factors.


Assuntos
Doença Hepática Terminal/terapia , Hepatite B/complicações , Hepatite B/prevenção & controle , Transplante de Fígado , Adulto , Fatores Etários , Antivirais , Ácido Aspártico/genética , Carcinoma Hepatocelular , China/epidemiologia , DNA Viral , Quimioterapia Combinada , Doença Hepática Terminal/etiologia , Guanina/administração & dosagem , Guanina/análogos & derivados , Hepatite B/epidemiologia , Antígenos E da Hepatite B , Vírus da Hepatite B/genética , Humanos , Imunoglobulinas/administração & dosagem , Lamivudina/administração & dosagem , Neoplasias Hepáticas , Masculino , Metionina/genética , Pessoa de Meia-Idade , Mutação , Estudos Retrospectivos , Fatores de Risco , Prevenção Secundária , Fatores Sexuais , Tirosina/genética
12.
Huan Jing Ke Xue ; 33(10): 3336-43, 2012 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-23233957

RESUMO

This paper assessed the coastal environmental quality along the Beibu Gulf using a statistical approach, multi-biomarker pollution index (MPI). Samples of clam (Paphia undulata) and sediment were collected at nine sites from the intertidal zone of Beibu Gulf in April 2011. Nine biomarkers of response were measured both in gill and digestive gland in Paphia undulata, and twelve kinds of contaminants were measured in different sediment samples. According to the Pearson' s correlation coefficients between biomarker responses in Paphia undulata and contaminant levels in sediments, five biomarkers either in gill for oxidized glutathione (GSSG), reduced glutathione (GSH), glutathione S-transferase (GST) or in digestive gland for thiobarbituric acid reactive substances (TBARS) and glutathione peroxidase (GPx) were selected for MPI calculation. For each biomarker at each site, a response index was allocated, and the MPI value of this site was calculated as the sum of the response index of the five biomarkers. The results of the calculation (MPI values from 18 to 39) showed significant differences among sampling sites. The environmental quality of all sites ranged from class 1 (clean) to class 3 (lightly contaminated), and no site fell into class 4 (contaminated) or class 5 (heavily contaminated), indicating a good environmental quality in the intertidal zone of Beibu Gulf. However, the environmental quality at some sites (S1, S3, 54 and S7) fell into class 3 (lightly contaminated), indicating mild interference from human activities has occurred.


Assuntos
Biomarcadores/análise , Bivalves , Monitoramento Ambiental/métodos , Poluentes Químicos da Água/análise , Animais , China , Glutationa/análise , Glutationa Peroxidase/análise , Glutationa Transferase/análise , Metais Pesados/análise , Oceanos e Mares , Água do Mar/química , Poluentes Químicos da Água/toxicidade
13.
Ying Yong Sheng Tai Xue Bao ; 23(4): 1128-36, 2012 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-22803485

RESUMO

Biomarkers are applied worldwide in marine environmental assessment due to their "early warning" function to chemical pollutants. Several integrative index approaches such as multi-marker pollution index (MPI), integrated biomarker response (IBR), bioeffect assessment index (BAI), biomarker response index (BRI), and health assessment index (HIS), have been developed based on biomarkers. By transforming the complex alterations of biomarkers into a single class or value, these approaches have been so far the useful tools for assessing the environmental quality. This review summarized the establishment of evaluation indicator system, the calculation of integrative index, the grading of pollution status, and the practical applications of each approach, and discussed the existing problems in marine environmental assessment based on biomarker index. Some improving suggestions were also proposed.


Assuntos
Biomarcadores/análise , Monitoramento Ambiental/métodos , Água do Mar/análise , Poluentes Químicos da Água/análise , Ecossistema , Oceanos e Mares , Medição de Risco/métodos
14.
Yi Chuan ; 33(1): 54-9, 2011 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-21377959

RESUMO

To investigate the association between the single nucleotide polymorphisms (SNPs) in adiponectin gene promoter and essential hypertension (EH) in Chinese Korean and Han of Yanbian area, 220 EH patients and 268 normotensive control individuals were enrolled. PCR and direct DNA sequencing were used to determine the -11426A>G (rs16861194), -11391G>A (rs17300539), -11377C>G (rs62620185), -11156insCA (rs60806105), and -11043C>T (rs76786086) SNPs in the promoter region of adiponectin gene. Total cholesterol (TC), the triglyceride (TG), fasting plasma glucose (FPG), low density lipoprotein-cholesterol (LDL-C), and high density lipoprotein-cholesterol (HDL-C) levels were examined by oxi-dase method. The plasma adiponectin and insulin were measured by enzyme linked immunosorbent assay (ELISA). The results showed that: (1) SNPs of -11426A>G, -11377C>G, and -11156insCA were found and in Hardy-Weinberg equilib-rium (P>0.05), but not the case in -11391G>A and -11043C>T. (2) -11426A>G and -11156insCA were perfectly in link-age disequilibrium (D'=1; r2=1). (3) The allele G frequency of -11426A>G polymorphism in Chinese Korean (21.10%) was significantly higher than that in Chinese Han (12.50%), and also higher in EH group than in the control group of Chinese Han. The genotype and allele frequencies of -11377C>G showed no significant difference between the two groups ob-served. (4) The haplotype -11426G -11377C frequency in EH of Chinese Han was higher than in the control group (P<0.05). (5) The EH showed lower plasma adiponectin level compared with the control group (P<0.001) in both Chinese Korean and Han. Our results indicate that: (1) the perfect linkage disequilibrium of -11426A>G and -11156insCA is first reported, and the SNP of -11426A>G is associated with Chinese Han and Korean. (2) -11426 G and -11426G -11377C are risk factor and risk haplotype in Yanbian Chinese Han, but not in Chinese-Korean. (3) The lower hypoadiponectinemia is the important risk factors for EH in Chinese Korean and Han of Yanbian area. (4) There is no relationship between -11426A>G polymorphism and the plasma adiponectin level.


Assuntos
Adiponectina/genética , Hipertensão/genética , Polimorfismo de Nucleotídeo Único , Regiões Promotoras Genéticas , Adiponectina/sangue , Adulto , Idoso , China/etnologia , Frequência do Gene , Genótipo , Humanos , Coreia (Geográfico)/etnologia , Desequilíbrio de Ligação , Pessoa de Meia-Idade
15.
Artigo em Chinês | MEDLINE | ID: mdl-21110426

RESUMO

OBJECTIVE: To investigate the characteristics of HCV genotypes of Han and Korean in Yanbian area of Jilin Province. METHODS: The HCV RNA load and genotypes of the 119 chronic hepatitis C patients in Yanbian area of Jilin Province were determined by real-time PCR and LiPA. The differences of the HCV genotypes in Han and Korean cases, in severity of the diseases, in HCV-RNA load, and in the relation with type 2 diabetes mellitus were analyzed. RESULTS: There was no significant difference in the distribution of each HCV genotype between Han and Korean patients (P > 0.05) with chronic hepatitis C. The difference between HCV genotype and HCV-RNA load was not significant (P > 0.05). With and without type 2 diabetes mellitus in these patients. The distribution of HCV genotype was also not significantly different (P > 0.05). The type 1b of HCV genotype in the moderate to severe chronic hepatitis C patients accounted for 58.06%. It was different compared with mild chronic hepatitis C patients (P < 0.05). CONCLUSION: 1) The type 1b is the most popular HCV genotype in Yanbian area of Jilin Province, type 2a is the second and there are still a few other genotypes. 2) There is no significant difference in the distribution of HCV genotypes between Han and Korean cases. 3) The HCV genotypes has nothing to do with the load of HCV-RNA. 4) The distribution of HCV genotypes in chronic hepatitis C patients with and without diabetes mellitus is not significantly different. 5) Type 1b of HCV infection is relatively severe.


Assuntos
Hepacivirus/genética , Hepatite C/virologia , Adolescente , Adulto , Idoso , Diabetes Mellitus Tipo 2/complicações , Feminino , Genótipo , Hepatite C/complicações , Hepatite C/epidemiologia , Humanos , Coreia (Geográfico)/epidemiologia , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Adulto Jovem
16.
Guang Pu Xue Yu Guang Pu Fen Xi ; 29(10): 2756-9, 2009 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-20038054

RESUMO

Three molecularly imprinted polymers were synthesized with different functional monomers respectively, using methyl parathion as the template. These monomers are methacrylic acid, acrylamide and 4-vinylpyridine. According to the UV spectrum study the interaction between methyl parathion and 4-vinylpyridine was stronger than that of the others. Comparably, the infrared spectrum study showed the same results which indicated that 4-vinylpyridine could associate with the template at two different kinds of binding sites, the P-O-C and the -NO2 site, and is most likely to form steady covalent bonds with methyl parathion, while the other two monomers could only associate with the template at the P-O-C site. Furthermore, the infrared spectra of the synthesized polymers confirmed the existence of the functional groups in each kind of polymer, which might interact with the template.

17.
Ai Zheng ; 28(3): 255-61, 2009 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-19619439

RESUMO

BACKGROUND AND OBJECTIVE: The anti-tumor activity of dexamethasone derivatives (9-fluoro-16alpha-methyl-11,17-dihydroxy-3-oxo-1,4-androsladiene-17beta-carboxylic acid) is superior to that of dexamethasone. This study was to screen the proteins interacting with dexamethasone derivates, thus to explore the anti-tumor mechanism of dexamethasone derivates in vivo. METHODS: The bait plasmid pGBKT7-GRalpha-LBD was constructed. Screening of the target proteins interacting with dexamethasone derivatives was performed by yeast three-hybrid technique using human K562 cell cDNA library. RESULTS: The bait plasmid was successfully constructed. It produced a 31 ku bait protein with no toxicity, leakage and self-activation. Thirty-seven positive clones which interacted with dexamethasone derivatives were obtained from human K562 cell cDNA library, 20 of which were identified by re-transforming into yeast AH109 cells. CONCLUSION: Twenty positive clones interacting with dexamethasone derivates are identified in vivo.


Assuntos
Androstadienos/farmacologia , Dexametasona/análogos & derivados , Mapeamento de Interação de Proteínas , Proteínas Recombinantes de Fusão/metabolismo , Antineoplásicos Hormonais/farmacologia , Dexametasona/farmacologia , Biblioteca Gênica , Vetores Genéticos , Humanos , Células K562 , Plasmídeos , Ligação Proteica , Transformação Genética , Técnicas do Sistema de Duplo-Híbrido
18.
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue ; 19(7): 419-21, 2007 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-17631711

RESUMO

OBJECTIVE: To observe the pathological changes and investigate the correlation of hepatocyte apoptosis with cytochrome P450 2E1(CYP2E1) expression and oxygen free radical in alcoholic liver disease (ALD) in rat. METHODS: 40% ethanol in the dose of 8 g/kg body weight was given to rats by gavage twice daily for 8 weeks in model group (n=37), and rats in control group (n=33) received same volume of saline by gavage. At the end of the 8th week, the contents of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined. The pathological changes in the liver was observed under light microscope with hematoxylin and eosin (HE) staining, and hepatocyte apoptosis was detected by the terminal deoxynucleotidyl transferase mediated dUTP biotin nick and labeling (TUNEL) method. Expression of serum CYP2E1 was determined by polymerase chain reaction (PCR), the contents of malondialdehyde (MDA) and activities of superoxide dismutase (SOD) in serum were detected by thibabituric acid (TBA) quantifying method. RESULTS: The TUNEL positive cells were located around the central vein, and spotty and focal necrosis was found in the ALD group. The apoptotic index (AI) in the ALD group was significantly higher than in the control group (P<0.05). To express in CYP2E1, the allelic frequency of c1 and c2 were 91.65%, and 8.35% respectively, in control group, while the allelic frequency of c1 and c2 were 53.35% and 46.65%, respectively, in ALD group, and there were significantly differences between two groups (all P<0.05). The content of MDA in serum had positive correlation with hepatocyte apoptosis index (MDA vs. AI, r( MDA ) =0.644), and the activity of SOD in serum had negative correlation with AI (SOD vs. AI, r( SOD ) =-0.511, all P<0.05) in the ALD group, and there was negative correlation between MDA and SOD (r=-0.582, P<0.05). CONCLUSION: Chronic alcohol administration induced alcoholic liver disease and liver dysfunction, and hepatocyte apoptosis is enhanced. Rsa I and Pst I RFLPs are related with ALD in model rats, and c2 gene might be related with the development of ALD. The content of MDA and activity of SOD play an important role in the process of hepatocyte apoptosis and lipid peroxidation process.


Assuntos
Apoptose , Citocromo P-450 CYP2E1/metabolismo , Hepatócitos/patologia , Hepatopatias Alcoólicas/patologia , Estresse Oxidativo , Animais , Modelos Animais de Doenças , Fígado/metabolismo , Fígado/patologia , Hepatopatias Alcoólicas/metabolismo , Masculino , Ratos , Ratos Wistar
19.
Huan Jing Ke Xue ; 27(9): 1829-34, 2006 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-17117641

RESUMO

A biosensor based on the flow injection system was constructed with the immobilized AChE from Scomberomorus niphonius (Curier) as identification element and a pH electrode as transducer. When phosphate buffer was used as carrier liquid, a good reproducibility (RSD = 1.427% , n=10) of the biosensor response was obtained after the substrate was injected repetitively. After an incubation time of 20 min, the calibration graph to methyl-parathion is linear (r = 0.9986) when its concentration ranges from 4.29 x 10(-10) mol x L(-1) to 4.29 x 10(-8) mol x L(-1) , and the detection limit is 1.3 x 10(-10) mol x L(-1). However, the sensitivity of this biosensor to methyl-parathion when using clean seawater as carrier liquid is not as good as that using phosphate buffer. But after preoxidation of methyl-parathion with NaClO as oxidant, the detection limit of the biosensor to methyl-parathion in seawater can be improve to 2.16 x 10(-7) mol x L(-1).


Assuntos
Acetilcolinesterase/metabolismo , Técnicas Biossensoriais , Enzimas Imobilizadas/metabolismo , Compostos Organofosforados/análise , Poluentes Químicos da Água/análise , Eletroquímica , Monitoramento Ambiental/instrumentação , Monitoramento Ambiental/métodos , Praguicidas/análise , Reprodutibilidade dos Testes , Água do Mar/análise
20.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 22(4): 507-9, 2006 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-16806018

RESUMO

AIM: To prepare the fusion protein of a single chain variable fragment 637 (scFv637) against human acetylcholine receptor (AChR) and human serum albumin (HSA) to increase the stability of scFv637. METHODS: HSA gene was amplified by PCR and cloned into vector pHEN2 containing a scFv637 gene to construct recombinant vector pHEN2-scFv637-HSA and then transformed into E. coli HB2151 for expression. The expression of fusion protein in periplasm of E. coli was detected by dot hybridization. Relative molecular mass of fusion protein was checked by SDS-PAGE and Western blot. RESULTS: Agarose gel electrophoresis analysis showed that the size of HSA gene and fusion gene was about 1,770 bp and 7,054 bp, respectively. DNA sequencing proved that the nucleotide sequence of constructed scFv637-HSA gene was correct and it was cloned into the open reading frame (ORF) of pHEN2. scFv637-HSA fusion protein only existed in periplasm of E. coli HB2151 transformed with pHEN2-scFv637-HSA. SDS-PAGE and Western blot analysis indicated that relative molecular mass of fusion protein was about 95,900. CONCLUSION: The scFv637-HSA fusion protein can be successfully expressed in E. coli HB2151, which provides a sound basis for further research into its function and clinical application.


Assuntos
Escherichia coli/genética , Região Variável de Imunoglobulina/biossíntese , Região Variável de Imunoglobulina/imunologia , Receptores Colinérgicos/imunologia , Proteínas Recombinantes de Fusão/biossíntese , Proteínas Recombinantes de Fusão/imunologia , Albumina Sérica/imunologia , Animais , Western Blotting , Clonagem Molecular , Eletroforese em Gel de Poliacrilamida , Expressão Gênica , Humanos , Região Variável de Imunoglobulina/análise , Região Variável de Imunoglobulina/genética , Camundongos , Proteínas Recombinantes de Fusão/análise , Proteínas Recombinantes de Fusão/genética
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