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1.
Rev Bras Enferm ; 72(suppl 3): 3-8, 2019 Dec.
Artigo em Inglês, Português | MEDLINE | ID: mdl-31851228

RESUMO

OBJECTIVE: The present study evaluated the anthropometric and metabolic profiles of preterm infants (PT) born from mothers with urinary tract infections (UTI) and mothers with hypertensive disorders (HD). METHOD: This was a longitudinal prospective study conducted between May 2015 and August 2016. First, 59 mothers with premature birth were included; after excluding 29 mothers, two subgroups were created: UTI-mothers (n=12) and HD-mothers (n=18). The anthropometric and metabolic variables of mothers and their respective PT were analyzed at birth and at 6 months of corrected age (CA). RESULTS: Plasma triglyceride levels were higher among HD-mothers and their respective PT in comparison with UTI-mothers and their PT at 6 m of CA. CONCLUSION: Plasma triglyceride level is an important metabolic biomarker in HD-mothers resulting in higher triglyceride levels among PT at the CA of 6 m, suggesting an early programming effect of maternal hypertension.

2.
Lasers Med Sci ; 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31729609

RESUMO

Rheumatoid arthritis denotes hyperplasia and intense inflammatory process. Treatment involves exercise protocols and use of resources such as low-level laser therapy (LLLT) to modulate the inflammatory process and maintain physical capacity. The objective was to investigate whether treatment with LLLT and exercise modulates the inflammatory process and peripheral functionality. Sample is composed of 128 male rats, separated into three groups, control, treated and untreated, in the acute and chronic period of the disease with 64 animals in each group, divided into 8 subgroups with n = 8. The animals were immunized with injection at the base of the tail and 7 days after intra-articular injection with complete Freund adjuvant (CFA) for lesion groups, and saline solution for the controls. Joint disability was evaluated by PET (paw elevation time) and joint edema and treated with LLLT and/or resisted stair climbing exercise. Normality Shapiro-Wilk test, ANOVA mixed for the functional analyses, and ANOVA one-way for the variables of cellular differentiation, with Bonferroni post hoc, p = 5% were used. For the evaluations of joint disability and nociception, there was a significant difference between the evaluations, the groups, and the interaction groups-evaluations. The treated groups showed recovery of functionality; it is still verified that laser therapy increased the nociceptive threshold of the chronic inflammatory period, and the exercise reflected in significant functional improvement and modulation of the inflammatory process both in the acute and chronic periods. LLLT, resistance exercise, or a combination of treatments had a positive effect on the modulation of the inflammatory process, reducing the migration of leukocytes, in addition to helping the return of peripheral functionality by reducing joint disability in a model of rheumatoid arthritis induced by CFA in rats.

3.
Autoimmune Dis ; 2018: 9856910, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30364021

RESUMO

One characteristic of autoimmune diseases (ADs) is the production of autoantibodies for extractable nuclear autoantigens, which may aid in the discrimination of the different types of autoimmune diseases and is related to different antinuclear antibody (ANA) patterns. The present study verified the profile of patient samples tested for extractable nuclear antigens (ENA) antibodies in a public hospital and correlated the ENA results with ANA patterns and patient diagnoses. The study reviewed data in the medical records of patients who underwent anti-ENA tests at a public hospital in the West of the State of Paraná from February 2011 to January 2017. Patients were classified according to age, ethnicity, gender, anti-ENA test results, ANA results, and the presence or absence of AD. Thirty-six (20.9%) samples of the 172 anti-ENA tests were positive, seven (4.1%) samples were undetermined, and 129 (75%) exhibited negative results. The ANA reagent was found in 84.3% of the anti-ENA-positive samples. The anti-SSA/Ro autoantibody exhibited the highest frequency in the group, 41.7% (15/36). The most common pattern was nuclear fine speckled, which was found in 24.3% of the samples. The association results indicated a significant relationship between ANA titer and diagnosis in the anti-ENA- and ANA-positive patients. The anti-ENA-negative patients were diagnosed with an AD in 35% (45/129) of the cases, and 75% (27/36) of the anti-ENA-positive patients were diagnosed with an AD. Systemic lupus erythematosus and scleroderma were the most common pathologies in the antigen-positive patients. The anti-ENA test is a good marker to aid in the complex clinical diagnosis of patients with autoimmune diseases.

4.
Acta Sci. Biol. Sci. ; 40: e41853, 2018.
Artigo | Sec. Est. Saúde SP, SESSP-IBPROD, Sec. Est. Saúde SP | ID: but-ib16020

RESUMO

Glycoalkaloids are important secondary metabolites accumulated by plants as protection against pathogens. One of them, a-tomatine, is found in high concentrations in green tomato fruits, while in the ripe fruits, its aglycone form, tomatidine, does not present a protective effect, and it is usual to find parasites of tomatoes like Phytomonasserpens in these ripe fruits. To investigate the sensitivity of trypanosomatids to the action of a-tomatine, we used logarithmic growth phase culture of 20 trypanosomatids from insects and plants and Trypanosoma cruzi. The lethal dose 50% (LD50) was determined by mixing 107 cells of the different isolates with a-tomatine at concentrations ranging from 10-3 to 10-8 M for 30 min at room temperature. The same tests performed with the tomatidine as a control showed no detectable toxicity against the same trypanosomatid cultures. The tests involved determination of the percentage (%) survival of the protozoan cultures in a Neubauer chamber using optical microscopy. The LD50 values varied from 10-4 to 10-6 M a-tomatine. Slight differences were detected among the LD50 values of the analyzed samples, and none of them showed evidence of resistance to the action of tomatinase, as shown by some pathogenic fungi.

5.
Int J Med Mushrooms ; 18(4): 313-20, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27481297

RESUMO

Ganoderma australe was studied to determine the composition of the cell wall, and polysaccharide fraction SK5 was obtained after freeze-thawing an aqueous 5% potassium hydroxide extraction. The monosaccharide composition of the SK5 fraction revealed by gas chromatography-mass spectrometry showed 81.3% glucose, and analyses by 13C nuclear magnetic resonance spectroscopy confirmed a ß-glucan with glycosidic links of the (1→3)-ß type and most likely 4-O substituted. In addition, the biological effect of the ß-glucan from G. australe was evaluated via in vitro cell cultures of peritoneal macrophages isolated from Swiss mice. Biological assays were assessed for toxicity and cell activation, interleukin-6 cytokine concentrations, and the ability to stimulate phagocytic activity. There was an increase in interleukin-6 by approximately 111% with 1.0 µg/mL of polysaccharide, and phagocyte activity was increased in all concentrations examined, obtaining 52.3% with 0.25 µg/mL polysaccharide. The results indicate that a ß-(1→3)-glucan isolated from G. australe can be classified as a biological response modifier.


Assuntos
Ganoderma/química , Fatores Imunológicos/farmacologia , Interleucina-6/metabolismo , Fagocitose/efeitos dos fármacos , Polissacarídeos/farmacologia , beta-Glucanas/farmacologia , Animais , Parede Celular/química , Macrófagos Peritoneais/efeitos dos fármacos , Macrófagos Peritoneais/imunologia , Camundongos
6.
Rev. bras. reumatol ; 55(2): 181-184, Mar-Apr/2015. graf
Artigo em Português | LILACS | ID: lil-746147

RESUMO

Paciente do sexo feminino, queixando-se de astenia e dor em hipogastro, foi admitida no pronto-socorro do Hospital Universitário do Oeste do Paraná (HUOP). Durante a anamnese relatou tratamento de infecção crônica pelo vírus da hepatite C (VHC) com inteferon peguilado e ribavirina. Dentre os exames laboratoriais solicitados, a pesquisa de autoanticorpos contra antígenos celulares (PAAC-HEp-2), conhecido tradicionalmente como fator antinúcleo (FAN), apresentou fluorescência em forma de bastões e/ou anéis no citoplasma das células. Esse padrão é caracterizado por bastões de 3-10 µm e anéis com 2-5 µm de diâmetro espalhados através do citoplasma da célula. Portanto, esse novo padrão tem sido designado como "bastões e anéis" (traduzido do inglês: Rods and Rings, RR). O alvo antigênico dessa reação foi identificado como inosina-5'-monofosfato desidrogenase tipo 2 (IMPDH2) que é uma enzima chave na síntese de nucleotídeos púricos. A enzima IMPDH2 agregada ou modificada em forma de RR nos pacientes tratados com ribavirina pode tornar-se antigênica e induzir uma resposta autoimune. É possível que o interferon alfa estimule a ocorrência de reatividade anti-RR aparentemente induzida pela ribavirina. Até o momento não se sabe por que o padrão RR em células HEp-2 ocorrem apenas em uma fração de pacientes portadores do VHC. Os dados apresentados em trabalhos anteriores possibilitam afirmar que esses anticorpos associados ao padrão RR estão fortemente relacionados com o tratamento da hepatite C. Além disso, pode-se afirmar que a ocorrência de reatividade anti-RR é promovida pela terapia combinada com interferon alfa e ribavirina.


Female patient, complaining of weakness and pain in hypogastric, was admitted to the emergency department of the University Hospital of the West of Paraná (HUOP). During the interview reported treatment of chronic infection with hepatitis C virus (HCV) with peginterferon and ribavirin. Among the laboratory tests ordered, the search for self-antibodies against cellular antigens, traditionally known as antinuclear factor, showed fluorescence shaped like rods and/or rings in the cytoplasm of cells. This study attempts to clarify the relationship between this pattern not yet completely understood and the clinical picture of the patient. This pattern is characterized by 3–10 µm rods or rings with 2–5 µm in diameter scattered throughout the cytoplasm of the cell. Therefore, this new standard has been designated as "rods and rings" (RR). The antigenic target of this reaction was identified as inosine-5'-monophosphate dehydrogenase type 2 (IMPDH2) which is a key enzyme in the synthesis of purine nucleotides. The IMPDH2 enzyme aggregated or modified shaped RR in those patients treated with ribavirin may become antigenic and induce an autoimmune response. It is possible that interferon alpha stimulates the occurrence of anti-RR reactivity apparently induced by ribavirin. So far it is not known why the standard RR in HEp2 cells occurs only in a fraction of patients with HCV. Previous studies presented in this paper allow affirming that these antibodies associated with the standard RR are strongly related to hepatitis C. Moreover, it can be stated that the occurrence of anti-RR reactivity is promoted by combination therapy with interferon and ribavirin.


Assuntos
Humanos , Feminino , Antivirais/uso terapêutico , Citoplasma/ultraestrutura , Hepatite C Crônica/tratamento farmacológico , Hepatite C Crônica/patologia
7.
Rev Bras Reumatol ; 55(2): 181-4, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25476473

RESUMO

Female patient, complaining of weakness and pain in hypogastric, was admitted to the emergency department of the University Hospital of the West of Paraná (HUOP). During the interview reported treatment of chronic infection with hepatitis C virus (HCV) with peginterferon and ribavirin. Among the laboratory tests ordered, the search for self-antibodies against cellular antigens, traditionally known as antinuclear factor, showed fluorescence shaped like rods and/or rings in the cytoplasm of cells. This study attempts to clarify the relationship between this pattern not yet completely understood and the clinical picture of the patient. This pattern is characterized by 3-10 µm rods or rings with 2-5 µm in diameter scattered throughout the cytoplasm of the cell. Therefore, this new standard has been designated as "rods and rings" (RR). The antigenic target of this reaction was identified as inosine-5'-monophosphate dehydrogenase type 2 (IMPDH2) which is a key enzyme in the synthesis of purine nucleotides. The IMPDH2 enzyme aggregated or modified shaped RR in those patients treated with ribavirin may become antigenic and induce an autoimmune response. It is possible that interferon alpha stimulates the occurrence of anti-RR reactivity apparently induced by ribavirin. So far it is not known why the standard RR in HEp2 cells occurs only in a fraction of patients with HCV. Previous studies presented in this paper allow affirming that these antibodies associated with the standard RR are strongly related to hepatitis C. Moreover, it can be stated that the occurrence of anti-RR reactivity is promoted by combination therapy with interferon and ribavirin.


Assuntos
Antivirais/uso terapêutico , Citoplasma/ultraestrutura , Hepatite C Crônica/tratamento farmacológico , Hepatite C Crônica/patologia , Feminino , Humanos
8.
Gen Dent ; 61(4): 10-3, 2013 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-23823336

RESUMO

Disinfecting dental impressions is necessary to decrease the risk of cross-contamination in dental offices. Ultrasonic nebulization has been mentioned as a microbicidal technique that can be used to disinfect contaminated dental impressions. This study compared the microbicidal effect of 2% glutaraldehyde and 0.2% peracetic acid for the disinfection of dental impressions made with vinyl polysiloxane, using 2 disinfection methods: immersion and ultrasonic nebulization. Bactericial efficacy was examined using Staphylococcus aureus and Bacillus atrophaeus as indicators. Thirty impressions were obtained and distributed randomly in 5 groups (n = 6). Group 1 was immersed in 2% glutaraldehyde immersion for 10 minutes, Group 2 was immersed in 0.2% peracetic acid for 10 minutes, Group 3 underwent ultrasonic nebulization for 10 minutes in 2% glutaraldehyde solution, Group 4 underwent ultrasonic nebulization for 10 minutes in 0.2% peracetic acid solution, and Group 5 was a control group that received no disinfectant. Both solutions experienced a 100% reduction in microorganisms following ultrasonic nebulization, as did peracetic acid following immersion; however, immersion in glutaraldehyde demonstrated lower values of reduction in B atrophaeus group, with a statistically significant difference compared with the other experimental groups.


Assuntos
Desinfetantes de Equipamento Odontológico , Materiais para Moldagem Odontológica , Técnica de Moldagem Odontológica , Desinfetantes , Desinfecção , Glutaral , Staphylococcus aureus , Ultrassom
9.
Rev Bras Reumatol ; 53(1): 57-65, 2013 Feb.
Artigo em Inglês, Português | MEDLINE | ID: mdl-23588516

RESUMO

INTRODUCTION: Brazilian epidemiological studies on rheumatoid arthritis are scarce, thus all data currently available originate from the international literature. OBJECTIVES: To determine the incidence and some clinical and laboratory characteristics of patients with rheumatoid arthritis in the municipality of Cascavel, state of Paraná, Brazil. PATIENTS AND METHODS: Data were collected between August 2010 and July 2011 in all health services of the municipality of Cascavel that provided health care in Rheumatology: a university-affiliated hospital, a public outpatient clinic and four private clinics. RESULTS: We identified 38 patients diagnosed with rheumatoid arthritis, resulting in an estimated incidence of 13.4 cases per 100,000 inhabitants/year. Thirty two patients were females, whose mean age was 47.6 years. The age group with the highest incidence was over 40 years. The mean time between first symptoms and diagnosis was 12.4 months. Rheumatoid factor was positive in 68.4% of the patients, and 18.4% already had radiological abnormalities at diagnosis. The pharmacological treatment of patients was also assessed and proved to be in accordance with those found in the literature. CONCLUSION: The incidence of rheumatoid arthritis obtained in the municipality of Cascavel was lower than those reported in international studies.


Assuntos
Artrite Reumatoide/diagnóstico , Adolescente , Adulto , Idoso , Artrite Reumatoide/sangue , Artrite Reumatoide/epidemiologia , Brasil/epidemiologia , Feminino , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Adulto Jovem
10.
Rev. bras. reumatol ; 53(1): 61-65, jan.-fev. 2013. tab
Artigo em Português | LILACS | ID: lil-670984

RESUMO

INTRODUÇÃO: Estudos epidemiológicos brasileiros sobre artrite reumatoide são bastante escassos, e os dados existentes hoje são majoritariamente de literatura internacional. OBJETIVOS: Determinar a incidência e algumas características clínicas e laboratoriais de pacientes com artrite reumatoide em Cascavel, PR, Brasil. PACIENTES E MÉTODOS: Os dados foram coletados entre agosto de 2010 e julho de 2011 em todos os serviços de saúde do município que possuíam atendimento na especialidade de reumatologia: um hospital universitário, o Centro Regional de Especialidades do Consórcio Intermunicipal de Saúde do Oeste do Paraná (CRE-CISOP) e quatro clínicas privadas da cidade. RESULTADOS: Foram identificados 38 pacientes com diagnóstico de artrite reumatoide, resultando em uma incidência estimada de 13,4 casos/100.000 habitantes/ano. Trinta e dois pacientes eram do gênero feminino, com média de idade de 47,6 anos. A faixa etária com maior incidência foi > 40 anos. O tempo médio entre os primeiros sintomas e o diagnóstico foi de 12,4 meses. O fator reumatoide foi positivo em 68,4% dos casos, e 18,4% já apresentavam alterações radiológicas no momento do diagnóstico. O tratamento farmacológico dos pacientes também foi avaliado e mostrou estar de acordo com o encontrado na literatura. CONCLUSÃO: A incidência de artrite reumatoide obtida em Cascavel está abaixo das incidências observadas em estudos internacionais.


INTRODUCTION: Brazilian epidemiological studies on rheumatoid arthritis are scarce, thus all data currently available originate from the international literature. OBJECTIVES: To determine the incidence and some clinical and laboratory characteristics of patients with rheumatoid arthritis in the municipality of Cascavel, state of Paraná, Brazil. PATIENTS AND METHODS: Data were collected between August 2010 and July 2011 in all health services of the municipality of Cascavel that provided health care in Rheumatology: a university-affiliated hospital, a public outpatient clinic and four private clinics. RESULTS: We identified 38 patients diagnosed with rheumatoid arthritis, resulting in an estimated incidence of 13.4 cases per 100,000 inhabitants/year. Thirty two patients were females, whose mean age was 47.6 years. The age group with the highest incidence was over 40 years. The mean time between first symptoms and diagnosis was 12.4 months. Rheumatoid factor was positive in 68.4% of the patients, and 18.4% already had radiological abnormalities at diagnosis. The pharmacological treatment of patients was also assessed and proved to be in accordance with those found in the literature. CONCLUSION: The incidence of rheumatoid arthritis obtained in the municipality of Cascavel was lower than those reported in international studies.


Assuntos
Adolescente , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem , Artrite Reumatoide/diagnóstico , Artrite Reumatoide/sangue , Artrite Reumatoide/epidemiologia , Brasil/epidemiologia , Incidência
11.
Rev. odonto ciênc ; 27(3): 213-217, 2012. ilus, tab
Artigo em Inglês | LILACS, BBO - Odontologia | ID: lil-656787

RESUMO

PURPOSE: This study evaluated the rate of microbial contamination of toothbrushes used by students and established a protocol for the spraying of 0.12% chlorhexidine to decrease the bacterial presence on the bristles of the brushes. METHODS: We performed cultures on selective and nonselective media from toothbrushes used by Dentistry students from State University of West Paraná (Unioeste/PR) to identify and quantify the number of total and specific microorganisms. For the chlorhexidine test, a crossover and double-blind study was conducted, with a random selection of volunteers. Each experimental phase consisted of fourteen days of brushing and an interval of seven days between treatments. For the test, thirty volunteers, divided into three groups of 10, using new toothbrushes, sprayed with either water or 0.12% chlorhexidine gluconate at different intervals (once or three times daily) after brushing. For statistical analysis, we used main effects ANOVA, and a P-value<0.05 was considered significant. RESULTS: There was microbial growth on 91% of the used toothbrushes, with 81.3% showing growth of Streptococcus. In 56.3% of the brushes, Staphylococcus and Enterobacteriaceae developed. The use of the chlorhexidine spray only three times per day was significantly more effective than water. CONCLUSION: Even in a group that knows the ideal conditions for the storage of toothbrushes, much microbial contamination still exists on the toothbrushes. A lower bacterial load can be obtained from the simple and inexpensive act of spraying chlorhexidine on the toothbrush after every brushing of the day.


OBJETIVO: Este trabalho verificou o índice de contaminação microbiológica de escovas dentais em uso por universitários e estabeleceu um protocolo para o borrifamento de clorexidina 0,12% para diminuir a presença bacteriana nas cerdas das escovas. MÉTODOS: Foram realizadas culturas em meios seletivos e não seletivos de escovas dentais em uso por acadêmicos do curso de Odontologia da Unioeste/PR para identificar e quantificar microrganismos totais e específicos. Para o teste com a clorexidina, foi realizado um estudo cruzado, duplo-cego com seleção aleatória dos voluntários em que cada fase experimental consistia de quatorze dias de escovação e sete dias de intervalo entre os tratamentos. Para o teste, trinta voluntários, divididos em três grupos de 10, utilizando escovas dentais novas, realizavam o borrifamento de água ou gluconato de clorexidina 0,12% com periodicidades diferentes (uma vez ou três vezes ao dia) seguidos à escovação. Para análise estatística foi utilizado uma ANOVA de efeitos principais e P<0,05 foi considerado significativo. RESULTADOS: Houve crescimento microbiano em 91% das escovas em uso, com 81,3% de crescimento de Streptococcus. Em 56,3% das escovas houve desenvolvimento de Staphylococcus e de enterobactérias. Quanto à eficácia da clorexidina somente utilizando três borrifamentos diários obteve diferença significativa em relação ao grupo que utilizou água. CONCLUSÃO: Mesmo em um grupo que tem conhecimento sobre as condições ideais para o armazenamento de escovas dentais, uma alta contaminação microbiana ainda existe. Uma menor carga bacteriana pode ser obtida a partir do uso de spray de clorexidina na escova após cada escovação diária.


Assuntos
Humanos , Masculino , Feminino , Antissepsia/métodos , Contaminação , Clorexidina/uso terapêutico , Enterobacteriaceae , Staphylococcus
12.
Rev Bras Reumatol ; 51(3): 231-9, 2011.
Artigo em Inglês, Português | MEDLINE | ID: mdl-21625811

RESUMO

INTRODUCTION: Brazilian epidemiological studies on systemic lupus erythematosus (SLE) are scarce, and currently available data originate almost entirely from international literature. OBJECTIVES: To determine the incidence and some clinical and laboratory characteristics of patients with SLE in the municipality of Cascavel, state of Paraná, Brazil. PATIENTS AND METHODS: Data were collected from August 2007 to July 2008 in all health services of Cascavel providing health care in rheumatology: a university-affiliated hospital, a public outpatient clinic, and three private clinics. RESULTS: The study identified 14 patients diagnosed with SLE, which resulted in an estimated incidence of 4.8 cases/100,000 inhabitants/year. All patients were female, and the mean age was 41.5 years. The highest incidence of disease occurred between 30 and 39 years of age, and 92.8% of patients met at least four of the 11 American College of Rheumatology (ACR) criteria for diagnosis of SLE. The drug treatment of patients was also assessed and proved to be in accordance with the Brazilian Consensus for Treatment of SLE. CONCLUSION: The incidence obtained in the municipality of Cascavel is close to those reported in international studies.


Assuntos
Lúpus Eritematoso Sistêmico/epidemiologia , Adulto , Idoso , Brasil/epidemiologia , Feminino , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Saúde da População Urbana , Adulto Jovem
13.
Rev. bras. reumatol ; 51(3): 235-239, maio-jun. 2011. tab
Artigo em Português | LILACS | ID: lil-588179

RESUMO

INTRODUÇÃO: Estudos epidemiológicos brasileiros sobre o lúpus eritematoso sistêmico (LES) são bastante escassos e os dados existentes hoje são praticamente todos de literatura internacional. OBJETIVOS: Determinar a incidência e algumas características clínicas e laboratoriais de pacientes com LES em Cascavel, Paraná - Brasil. PACIENTES E MÉTODOS: Os dados foram coletados entre agosto de 2007 e julho de 2008 em todos os serviços de saúde do município que possuíam atendimentos na especialidade de Reumatologia: um hospital universitário, um ambulatório público e três clínicas privadas da cidade. RESULTADOS: Foram identificados 14 pacientes com diagnóstico de LES, resultando em uma incidência estimada de 4,8 casos/100.000 habitantes/ano. Todos os pacientes eram do sexo feminino, com média de idade de 41,5 anos. A faixa etária com maior incidência foi a de 30 - 39 anos e 92,8 por cento apresentaram quatro ou mais dos 11 critérios do American College of Rheumatology (ACR) para o diagnóstico de LES. O tratamento farmacológico dos pacientes também foi avaliado e mostrou estar de acordo com o Consenso Brasileiro para o tratamento de LES. CONCLUSÃO: A incidência obtida em Cascavel/PR está próxima das incidências observadas em estudos internacionais.


INTRODUCTION: Brazilian epidemiological studies on systemic lupus erythematosus (SLE) are scarce, and currently available data originate almost entirely from international literature. OBJECTIVES: To determine the incidence and some clinical and laboratory characteristics of patients with SLE in the municipality of Cascavel, state of Paraná, Brazil. PATIENTS AND METHODS: Data were collected from August 2007 to July 2008 in all health services of Cascavel providing health care in rheumatology: a university-affiliated hospital, a public outpatient clinic, and three private clinics. RESULTS: The study identified 14 patients diagnosed with SLE, which resulted in an estimated incidence of 4.8 cases/100,000 inhabitants/year. All patients were female, and the mean age was 41.5 years. The highest incidence of disease occurred between 30 and 39 years of age, and 92.8 percent of patients met at least four of the 11 American College of Rheumatology (ACR) criteria for diagnosis of SLE. The drug treatment of patients was also assessed and proved to be in accordance with the Brazilian Consensus for Treatment of SLE. CONCLUSION: The incidence obtained in the municipality of Cascavel is close to those reported in international studies.


Assuntos
Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem , Lúpus Eritematoso Sistêmico/epidemiologia , Brasil/epidemiologia , Incidência , Saúde da População Urbana
14.
FEMS Immunol Med Microbiol ; 39(3): 257-64, 2003 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-14642311

RESUMO

The immune cross-reactivity between Trypanosoma cruzi, the protozoan that causes Chagas' disease, and Phytomonas serpens, a trypanosomatid that infects tomatoes, was studied. Sera from patients with Chagas' disease presented a strong reactivity with P. serpens antigens by conventional serological assays such as indirect immunofluorescence (IIF) and direct agglutination test (DAT), confirmed after cross-absorption experiments. The results show that this protozoan is highly immunogenic and that rabbit and mouse hyperimmune serum raised against T. cruzi or P. serpens was able to recognize both T. cruzi and P. serpens antigens in immunofluorescence and agglutination assays. The antigenic cross-reactivity between T. cruzi and P. serpens was also demonstrated in vivo. BALB/c mice immunized by the intraperitoneal or oral route with P. serpens and later challenged with a lethal inoculum of T. cruzi blood forms showed a significant decrease in parasitemia and increase in survival compared to controls. A practical implication of these findings is that the ingestion by humans or animals of living plant trypanosomatids present in naturally infected edible fruits could potentially prime the immune response to T. cruzi antigens and interfere with the development of T. cruzi infection.


Assuntos
Antígenos de Protozoários/imunologia , Doença de Chagas/imunologia , Trypanosoma cruzi/imunologia , Trypanosomatina/imunologia , Testes de Aglutinação , Animais , Doença de Chagas/parasitologia , Doença de Chagas/prevenção & controle , Reações Cruzadas/imunologia , Feminino , Técnica Indireta de Fluorescência para Anticorpo , Humanos , Imunização , Lycopersicon esculentum/parasitologia , Camundongos , Camundongos Endogâmicos BALB C , Parasitemia , Coelhos , Análise de Sobrevida
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