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Biomaterials ; 222: 119442, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31491561


Polyelectrolyte modified iron oxide nanoparticles have great potential applications for clinical magnetic resonance imaging (MRI) and anemia treatments, however, possible associated heart toxicity is rarely reported. Here, polyacrylic acid (PAA)-coated Fe3O4 nanoparticles (PION) were synthesized and lethal reactions appeared when it was applied in vivo. The investigation of underlying mechanism showed that PION could break electrolyte balance and further resulted in serious heart failure, which was observed under color doppler ultrasound and dynamic vector blood flow technique. The results demonstrated that PION had a strong absorption tendency for divalent ions and the maximum tolerated dose (MTD) was lower than 100 mg/kg. From electrocardiography (ECG), PION presented an obvious impact on CaV1.2 ion channel, which leading to fatal arrhythmia. An appropriate solution for preventing this deadly effect was pre-chelation Ca2+ (n (Ca): n (COOH) = 3: 8) to PION (PION-Ca), which displayed much higher cardiac and electrophysiological safety when sealing the binding point of divalent cation ions with PAA. The injection in Beagle dogs further confirmed the safety of PION-Ca. This study explored the mechanism and offered a solution for cardiac toxicity induced by PAA-coated nanoparticles, which guides for enhancing the safety of such polyelectrolyte decorated nanoparticles and provides assurance for clinical applications.

Biosens Bioelectron ; 66: 321-6, 2015 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-25437370


Vicinal-dithiol-containing Proteins (VDPs) are overexpressed in cancer cells and become a potential biomarker for aggressive tumors, the synthesized 2-p-aminophenyl-1, 3, 2-dithiarsenolane (VTA2) is proved to be a highly selective ligand for vicinal dithiols of VDPs in cells. In this report, we developed a new cytosensor based on three-dimensional (3D)-like VTA2-conjugated multiwalled carbon nanotubes (VTA2@MWCNTs) array modified indium tin oxide (ITO) electrode for sensitive and selective detection of VDPs-overexpression tumor cells. The layer-by-layer assembling and cellular detection performances of the 3D-VTA2@MWCNTs-based cytosensor were investigated by atomic force microscopy (AFM) and electrochemical methods including cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). Attributed to signal amplification and targeting recognition of the 3D-structured MWCNTs@VTA2, the fabricated cytosensor showed high specificity and sensitivity to the detection of VDPs-overexpression HL-60 cells ranging from 2.7×10(2) to 2.7×10(7)cellsmL(-1) with a low detection limit of ca. 90cellsmL(-1). Furthermore, the captured cancer cells on the cytosensor also could be directly visualized by optical microscopy technology. This proposed 3D-MWCNTs array-based cytosensing strategy provides a simple, sensitive approach for non-invasive cell detection, presenting potential applications in cancer diagnosis.

Técnicas Biossensoriais/instrumentação , Nanotubos de Carbono/química , Neoplasias/diagnóstico , Proteínas/química , Tolueno/análogos & derivados , Compostos de Anilina/química , Arsenicais/química , Biomarcadores Tumorais/química , Técnicas Eletroquímicas/instrumentação , Desenho de Equipamento , Células HL-60 , Humanos , Nanotubos de Carbono/ultraestrutura , Tolueno/análise