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1.
Acta Naturae ; 2(2): 36-59, 2010 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-22649640

RESUMO

This review focuses on new trends in nucleoside biotechnology, which have emerged during the last decade. Continuously growing interest in the study of this class of compounds is fueled by a number of factors: ( i ) a growing need for large-scale production of natural 2 ' -deoxy- ß -D-ribonucleosides as well as their analogs with modifications in the carbohydrate and base fragments, which can then be used for the synthesis and study of oligonucleotides, including short-interfering RNA (siRNA), microRNA (miRNA), etc.; ( ii ) a necessity for the development of efficient practical technologies for the production of biologically important analogs of natural nucleosides, including a number of anticancer and antiviral drugs; ( iii ) a need for further study of known and novel enzymatic transformations and their use as tools for the efficient synthesis of new nucloside analogs and derivates with biomedical potential. This article will review all of these aspects and also include a brief retrospect of this field of research.

2.
Vopr Virusol ; 46(5): 40-2, 2001.
Artigo em Russo | MEDLINE | ID: mdl-11715709

RESUMO

High antiviral activity of 2'-deoxy-2'fluoroguanosine (2'-D-2'-FG) was observed in chicken embryo cells infected with FPV/Rostock/34 (H7N1) influenza virus and herpes simplex virus (HSV) type I (strain 1C). 50% inhibitory concentration (IC50) of 2'-D-2'-FG was 1.44 microM for FPV and 0.093 microM for HSV. IC50 of remantadine hydrochloride, ribavirin (FPV), and acycloguanosine (HSV) were < 0.46, 14.4, and 0.08 microM, respectively. Anti-HSV activity was confirmed in Vero cells: IC50 < 0.34 microM for 2'-D-2'-FG and IC50 < 0.044 microM for acycloguanosine.


Assuntos
Antivirais/farmacologia , Desoxiguanosina/análogos & derivados , Desoxiguanosina/farmacologia , Herpesvirus Humano 1/efeitos dos fármacos , Orthomyxoviridae/efeitos dos fármacos , Animais , Embrião de Galinha , Testes de Sensibilidade Microbiana , Células Vero
3.
Nucleosides Nucleotides Nucleic Acids ; 19(10-12): 1861-84, 2000.
Artigo em Inglês | MEDLINE | ID: mdl-11200279

RESUMO

Synthesis of 9-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)-adenine (7, ara-A2'F) and -guanine (12, ara-G2'F) was accomplished via the condensation of 2,6-dichloropurine (1) with 2-deoxy-2-fluoro-1,3,5-tri-O-benzoyl-alpha-D-arabinofuranose (2) as a key chemical step. Condensation of silylated N6-benzoyladenine (6) with 2 gave, after deblocking and chromatographic separation, ara-A2'F (7) (14%), it's alpha-anomer 8 (14%) and N7-alpha-isomer 9 (25%). The PSEUROT analysis of N9-betaD-arabinosides 7 and 12 manifested slight preference for the S rotamer (64%) for the former, and an equal population of the N and S rotamers for the latter. The arabinosides 7 and 12 were used for the preparation of the respective phosphoamidite building blocks 13 and 14 for automated oligonucleotide synthesis. Four 15-mer oligonucleotides (ONs) complementary to the initiation codon region of firefly luciferase mRNA were prepared: unmodified 2'-deoxy-ON (AS 1) and containing (i) ara-A2'F instead of the only A (AS2), (ii) ara-G2'F vs. 3-G from the 5'-terminus (AS3), and (iii) both arabinosides at the same positions (AS4). All these ONs display practically the same (i) affinity to both complementary DNA and RNA, and (ii) ability to inhibit a luciferase gene expression in a cell-free transcription-translation system.


Assuntos
Arabinonucleosídeos/química , Hibridização de Ácido Nucleico , Oligonucleotídeos Antissenso/química , Oligonucleotídeos Antissenso/síntese química , Oligonucleotídeos/química , Sequência de Bases , Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos , Luciferases/genética , Espectroscopia de Ressonância Magnética , Oligonucleotídeos Antissenso/farmacologia , Termodinâmica
4.
Nucleosides Nucleotides Nucleic Acids ; 19(10-12): 1885-909, 2000.
Artigo em Inglês | MEDLINE | ID: mdl-11200280

RESUMO

The synthesis of 1-(2-deoxy-3-O-phosphonomethyl-beta-D-erythropentofuranosyl)thymine (17) and its alpha-anomer 18 is described. Attempts to prepare 1-[2-deoxy-3-O-(pyrophosphoryl)phosphonomethyl-beta-D-erythro-pentofuranosyl]thymine (19) by an activation of the respective phosphonate 17 with 1,1'-carbonyldiimidazole (Im2CO) resulted in the quantitative formation of the corresponding pyrophosphonate derivative 21 (Scheme 2). Activation of inorganic pyrophosphate with Im2CO followed by the condensation with the phosphonates 17 and 18 afforded the desired analogues of nucleoside triphosphate 19 (35%) and its alpha-anomer 20 (27%) along with the respective pyrophosphonate derivatives 21 (37%) and 24 (38%) (Scheme 3). It was found that compounds 19 and 20 display (i) no substrate properties toward calf thymus terminal deoxynucleotidyl transferase (TDT) and AMV reverse transcriptase, and (ii) moderate substrate activity with E. coli DNA polymerase I (Klenow fragment).


Assuntos
DNA Polimerase Dirigida por DNA/metabolismo , Difosfatos/química , Timidina/síntese química , Animais , Sequência de Bases , Bovinos , Espectroscopia de Ressonância Magnética , Especificidade por Substrato , Timidina/análogos & derivados , Timidina/metabolismo
6.
Nucleosides Nucleotides ; 18(4-5): 687-8, 1999.
Artigo em Inglês | MEDLINE | ID: mdl-10432666

RESUMO

Chemical and enzymatic methods were employed for the synthesis of the title compound, 2'F-Guo 7. High antiviral activity of 2'F-Guo was established in chick embryo cells infected with influenza virus FPV/Rostock/34 (H7N1) and herpes simplex virus (HSV) type I (1C strain).


Assuntos
Antivirais/síntese química , Antivirais/farmacologia , Desoxiguanosina/análogos & derivados , Animais , Embrião de Galinha , Desoxiguanosina/síntese química , Desoxiguanosina/farmacologia , Herpesvirus Humano 1/efeitos dos fármacos , Orthomyxoviridae/efeitos dos fármacos
7.
Bioorg Khim ; 25(4): 282-9, 1999 Apr.
Artigo em Russo | MEDLINE | ID: mdl-10422595

RESUMO

The effect of core trimers, (2'-5')-analogues of oligoadenylic acid containing 9-(3-deoxy-3-fluoro-beta-D-xylofuranosyl)adenine (AF) and 3'-deoxy-3'-fluoroadenosine (AF) in various positions of the oligomer chain, on the lytic activity of human natural killer cells (NK cells) was studied in three different ways. The cellular cytotoxicity was determined using a highly sensitive nonradioactive approach employing a chelate europium-diethylenetriamino-pentaacetic acid complex (Eu-DTPA). It was shown that all fluorodeoxyanalogues enhance the lytic activity of intact NK lymphocytes, which follows from the lysis rate constant k2. At the same time, the substitution of either the central adenosine fragment or (to a greater extent) the 5'-terminal residue of (2'-5')A3 with AF causes a decrease in the number of active NK cells, which, unlike the case of the natural core trimer, leads to a loss of the capacity to increase the activity of NK. By contrast, isomeric ribo-analogues. (2'-5')(AF)A2 and (2'-5')A(AF)A, and trimers with the 2'(3')-terminal nucleotide substituted by AF or AF increased the activity of NK cells with an effectiveness close to or higher than the natural trimer (2'-5')A3. Inasmuch as isomeric xylo- and ribo-3'-deoxy-3'-fluoroanalogues of (2'-5')A3 are stereochemically modified oligomers, the data unambiguously suggest that the spatial structure of these trimers affects the increase in the lytic activity of NK cells.


Assuntos
Citotoxicidade Imunológica/efeitos dos fármacos , Desoxiadenosinas/farmacologia , Células Matadoras Naturais/imunologia , Humanos , Células Matadoras Naturais/química
8.
Bioorg Khim ; 25(9): 679-89, 1999 Sep.
Artigo em Russo | MEDLINE | ID: mdl-10624560

RESUMO

To elucidate the antibody-(2'-5')oligoadenylate relation to the mode of the hapten-immunogen conjugation, a new (2'-5')oligoadenylic acid trimer derivative containing a 2'-terminal N6-(5-carboxypentyl)adenosine and its 125I-labeled immunogenic conjugate were synthesized. The immunization with this conjugate and with a conjugate based on the 2',3'-O-[1-(2-carboxyethyl)]ethylidene derivative of the (2'-5')triadenylic acid gave antisera with different affinities toward modified (2'-5')oligonucleotides. Epitopes involved in the (2'-5')oligomer-binding to different antisera were found.


Assuntos
Nucleotídeos de Adenina/imunologia , Anticorpos/imunologia , Haptenos/imunologia , Oligorribonucleotídeos/imunologia , Vacinas Sintéticas/imunologia , Reações Cruzadas , Haptenos/química , Espectroscopia de Ressonância Magnética , Radioimunoensaio
9.
Bioorg Khim ; 25(10): 763-7, 1999 Oct.
Artigo em Russo | MEDLINE | ID: mdl-10645479

RESUMO

Mouse antibodies to (2'-5')oligoadenylates were obtained by the immunization of animals with the (2'-5')oligoadenylic acid trimer conjugated with bovine serum albumin through a 2',3'-levulinic acid residue. Using radioimmunoassay, the reactivity of mouse polyclonal antibodies to the (2'-5')oligoadenylic acid trimer was studied for the trimer analogues containing 9-(3-deoxy-3-fluro-beta-D- xylofuranosyl)adenine and 3'-deoxy-3'-fluoro-adenosine in various positions of the chain. It was found that (a) the three-dimensional structure of short oligonucleotides is an important factor in the antibody recognition; (b) antibodies are more sensitive to modifications of the 5'-terminal and central ribose fragments of the (2'-5')oligoadenylic acid trimer; (c) the 3'-hydroxyl group plays a secondary role in the formation of the antigen determinant.


Assuntos
Nucleotídeos de Adenina/imunologia , Anticorpos/imunologia , Oligorribonucleotídeos/imunologia , Nucleotídeos de Adenina/química , Animais , Anticorpos/química , Biopolímeros , Dicroísmo Circular , Camundongos , Oligorribonucleotídeos/química , Soroalbumina Bovina/imunologia , Estereoisomerismo
10.
Acta Biochim Pol ; 45(1): 87-94, 1998.
Artigo em Inglês | MEDLINE | ID: mdl-9701500

RESUMO

Two non-conventional analogues of ATP, 3'-deoxyadenosine-2'-triphosphate (3'-d-2'-ATP) and 2'-deoxyadenosine-3'-triphosphate (2'-d-3'-ATP), the syntheses of which are described, were examined as potential phosphate donors for the nucleoside kinases: 2'-deoxycytidine kinase (dCK), cytosolic thymidine kinase (TK1) and mitochondrial thymidine kinase (TK2). The reactions were monitored by means of a mixture of [gamma-32P]ATP and cold analogue, and/or with the use of 3H-labelled acceptors and cold donor. With dCK, using equimolar mixtures of ATP with each analogue, and dC as acceptor, phosphate transfer from 3'-d-2'-ATP and 2'-d-3'-ATP amounted to 34% and 14%, respectively. With each analogue used alone (each at concentration of 100 microM), phosphate transfer from 3'-d-2'-ATP was 55% that from ATP, and from 2'-d-3'-ATP 16%. With human TK2, and equimolar mixtures of [gamma-32P]ATP with each of the analogues, and 1 microM dT as acceptor, there was no detectable transfer from either analogue. But, when each analogue was used alone, phosphate transfer attained 11% and 5%, respectively, that for ATP alone. With the low affinity form of human TK1, and dT as acceptor, only low phosphate transfer occurred with either analogue used alone. Both compounds exhibited Michaelis-Menten kinetics (with significantly lower Vmax than ATP), while ATP exhibited cooperative kinetics with all three kinases.


Assuntos
Nucleotídeos de Desoxiadenina/química , Desoxicitidina Quinase/química , Timidina Quinase/química , Catálise , Humanos , Fosforilação
11.
New Microbiol ; 21(2): 141-6, 1998 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-9579338

RESUMO

The antiviral effect of 2',5'-trioligoadenylate (2',5'-A3) and some of its analogues was studied using several model cell culture systems and viruses: mice L929 fibroblast cells inoculated with vaccine virus, testicular piglet cells inoculated with Aueszki disease virus (strain BUK-628), and the same culture inoculated with a reference strain of transmissible gastroenteritis virus, strain Purdue-115. Our results suggest that both 2',5'-trioligoadenylate and its analogues are promising antiviral substances against DNA- and RNA-containing viruses.


Assuntos
Nucleotídeos de Adenina/farmacologia , Antivirais/farmacologia , Herpesvirus Suídeo 1/efeitos dos fármacos , Oligorribonucleotídeos/farmacologia , Vírus da Gastroenterite Transmissível/efeitos dos fármacos , Vírus Vaccinia/efeitos dos fármacos , Animais , Células Cultivadas/virologia , Fibroblastos/virologia , Camundongos , Testes de Sensibilidade Microbiana , Suínos/virologia
12.
New Microbiol ; 21(2): 197-201, 1998 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-9579344

RESUMO

The effect of trimeric 2',5'-oligoadenylic acid (2',5' A3) and its epoxy-derivative (2',5'-A2 (RAA) on human immunodeficiency virus (HIV-1) reproduction was studied. An HIV-1 infectivity titer decrease was shown on the model of limphoblastoid cells when the substances under study were used. The interferonogenic effect of both substances was discovered. 2',5'-A2 (RAA) inhibited the activity of retrovirus reverse transcriptase (a C-type).


Assuntos
Nucleotídeos de Adenina/farmacologia , Antivirais/farmacologia , HIV-1/efeitos dos fármacos , Oligorribonucleotídeos/farmacologia , DNA Polimerase Dirigida por RNA/efeitos dos fármacos , Repressão Enzimática , HIV-1/enzimologia , HIV-1/fisiologia , Humanos , Células Tumorais Cultivadas , Replicação Viral/efeitos dos fármacos
13.
Biochem Biophys Res Commun ; 245(2): 430-4, 1998 Apr 17.
Artigo em Inglês | MEDLINE | ID: mdl-9571169

RESUMO

To elucidate further the roles played by the adenine bases in the interaction of RNase L (EC 3.1.2.6) with the 2',5'-oligoadenylate 2-5A, p5'A2'(p5'A2')np5' A, a series of sequence-specific 1-deazaadenosine (c1A)-substituted analogues were synthesized and evaluated for their ability to bind to and activate human RNase L in comparison to earlier reported inosine-substituted congeners of 2-5A. Substitution of only the 5'-terminal adenosine of p5'A2'p5'A2 p5 A with c1A afforded an analogue with strongly diminished RNase L binding and activation ability, while replacement of the second or middle adenosine of p5 A2' p5'A2'p5' A had only a modest effect. In distinct contrast to p5'A2'p5'A2'p5'I, the c1A analogue with the third or 2'-terminal adenosine replacement approached parent p5' A2'p5'A2'p5' A in RNase L activation ability. These results permitted a further dissection of the role of various nucleotidic functional groups in the interaction of 2-5A with RNase L: specifically, that the 5'-terminal adenosine purine N-1 moiety is key for binding to RNase L, while the 2'-terminal adenosine N-6 exocyclic amino group is critical for RNase L activation.


Assuntos
Nucleotídeos de Adenina/farmacologia , Endorribonucleases/metabolismo , Oligorribonucleotídeos/farmacologia , Adenosina/análogos & derivados , Adenosina/metabolismo , Ativação Enzimática/fisiologia , Humanos , Estrutura Molecular , Oligorribonucleotídeos/metabolismo , Proteínas de Ligação a RNA , Proteínas Recombinantes/metabolismo , Tubercidina/química
15.
Carbohydr Res ; 285: 17-28, 1996 May 14.
Artigo em Inglês | MEDLINE | ID: mdl-9011376

RESUMO

The synthesis of some 3'-C-branched-3'-deoxy adenine nucleosides is described. Starting from the known 3-deoxy-3-C-(hydroxymethyl)-1,2;5,6-di-O-isopropylidene-alpha-D-allof uranose (1), a versatile glycosylating agent, namely 1,2-di-O-acetyl-5-O-benzoyl-3-deoxy-3-C-(mesyloxymethyl)-beta-D-ri bofuranose (6), was prepared in three steps. Condensation of the latter with bis(trimethysilylated) N6-benzoyladenine in the presence of tin(IV) chloride gave the N9-beta-nucleoside 7. Compound 7 was converted into (i) 9-[3-C-(azidomethyl)-3-deoxy-beta-D-ribofuranosyl]adenine (10), (ii) 9-[3-C-(azidomethyl)-2,3-dideoxy-beta-D-glycero-pent-2-enofuran osyl]adenine (14) and 9-[2-azido-3-C-(azidomethyl)-2,3-dideoxy-beta-D-arabinofuranosyl]a denine (15), and (iii) 9-[3-deoxy-2-O,3-C-(methylene)-beta-D-ribofuranosyl]adenine (16). None of the tested nucleosides showed marked cytostatic or antiviral activity in vitro.


Assuntos
Antivirais/síntese química , Desoxiadenosinas/síntese química , Desoxiadenosinas/farmacologia , Vírus/efeitos dos fármacos , Animais , Antivirais/química , Antivirais/farmacologia , Linhagem Celular , Dicroísmo Circular , Desoxiadenosinas/química , Humanos , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Nucleosídeos/química , Nucleosídeos/farmacologia
16.
Eur J Biochem ; 221(2): 759-68, 1994 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-8174555

RESUMO

A one- and two-dimensional NMR study has been performed on seven A(2'-5')A(2'-5')A fragments containing 9-(3'-fluoro-3'-deoxy-beta-D-xylofuranosyl)-adenine (AF) or 3'-fluoro-3'-deoxyadenosine (AF) residues at different positions, and on the corresponding monomers. A(2'-5')A(2'-5')A served as a reference compound. The fluoro substituent governs the conformation of the sugar ring: an AF residue displays mainly N-type sugar and the ring is considerably flattened (phi N approximately 30 degrees) compared to AF residues (phi S approximately 40 degrees), which exhibit almost pure S-type conformation. Moreover, in AF moieties the rotamer distribution around torsion angle gamma (O5'-C5'-C4'-C3') and the base orientation are influenced to a large extent by the presence of the fluorine substituent. The sugar rings of nonfluorinated residues in the trimers appear rather flexible. A possible correlation between the conformational characteristics of the fluorinated fragments and their biological activity has been found: the fragments that meet the prerequisites for binding to RNase L indeed show enhanced binding to this endonuclease. Furthermore, substitution of the 3'-OH group of the second residue by hydrogen or of the 3'-OH group of the 2'-terminal residue by fluorine or hydrogen results in increased resistance towards 2'-5'-phosphodiesterase.


Assuntos
Nucleotídeos de Adenina/química , Desoxiadenosinas/química , Endorribonucleases/metabolismo , Nucleotídeos de Adenina/farmacologia , Desoxiadenosinas/farmacologia , Espectroscopia de Ressonância Magnética , Conformação Molecular , Polímeros , Relação Estrutura-Atividade
17.
J Med Chem ; 34(7): 2195-202, 1991 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-2066992

RESUMO

A series of 3'-deoxy-3'-fluoro- and 2'-azido-2',3'-dideoxy-3'-fluoro-D-ribofuranosides of natural heterocyclic bases have been synthesized with the use of universal carbohydrate precursors, viz., 1-O-acetyl-2,5-di-O-benzoyl-3-deoxy-3-fluoro-D-ribofuranose and methyl 2-azido-5-O-benzoyl-2,3-dideoxy-3-fluoro-beta-D-ribofuranoside, respectively. The cytostatic and antiviral activity of the compounds was evaluated against a variety of tumor cell lines and DNA/RNA viruses, respectively. As the most active compound, from both a cytostatic and antiviral activity viewpoint, emerged 3'-deoxy-3'-fluoroadenosine. It inhibited the proliferation of some tumor cell lines (i.e. murine leukemia L1210 and human T-lymphocyte MT-4) at a concentration of 0.2-2 micrograms/mL, and proved inhibitory to the replication of positive-stranded RNA viruses (i.e. polio, Coxsackie, Sindbis, Semliki forest), double-stranded RNA viruses (i.e. reo), and some DNA viruses (i.e. vaccinia) at a concentration of 1-4 micrograms/mL, which is well below the cytotoxicity threshold (40 micrograms/mL).


Assuntos
Antineoplásicos/síntese química , Antivirais/síntese química , Didesoxinucleosídeos/síntese química , Hidrocarbonetos Fluorados/síntese química , Animais , Antineoplásicos/farmacologia , Antivirais/farmacologia , Linhagem Celular , Fenômenos Químicos , Química , Didesoxinucleosídeos/farmacologia , Humanos , Hidrocarbonetos Fluorados/farmacologia , Leucemia L1210/tratamento farmacológico , Testes de Sensibilidade Microbiana , Coelhos , Relação Estrutura-Atividade , Células Tumorais Cultivadas/efeitos dos fármacos
18.
Nucleic Acids Symp Ser ; (24): 67-70, 1991.
Artigo em Inglês | MEDLINE | ID: mdl-1841383

RESUMO

Rabbit antibodies to 2',5'-linked triadenylate were prepared by immunization with (2',5')A3 conjugated via the 2'3'-levulinic group, (2'5')A3-Lev, to BSA. New radioimmunoassay for (2',5')oligoadenylates was developed using 125I thyrosine labeled derivative of (2',5')A3-Lev. Reactivity of antibodies with phosphorothioate and seco analogs of oligoadenylates was studied. It was found that (i) stereospecific substitution of the diastereotopic oxygens with sulfur in the internucleotide phosphodiester linkages changes the immunoreactivity of such analogs; (ii) the seco analogs of oligoadenylates display in some cases a rather high reactivity.


Assuntos
Nucleotídeos de Adenina/imunologia , Antivirais/imunologia , Oligorribonucleotídeos/imunologia , Animais , Especificidade de Anticorpos , Antivirais/síntese química , Reações Cruzadas , Coelhos/imunologia , Relação Estrutura-Atividade
19.
Appl Microbiol Biotechnol ; 32(6): 658-61, 1990 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-1367439

RESUMO

Synthesis of 9-(beta-D-arabinofuranosyl)guanine (ara-G) from 1-(beta-D-arabinofuranosyl)cytosine (ara-C) and guanine, guanosine or 2'-deoxyguanosine (dG) by glutaraldehyde-treated Escherichia coli BM-11 cells is described. It is shown that the concentration of phosphate ions, molar ratio of substrates and pH of the reaction medium are factors affecting product yield. Under optimum conditions ara-G was produced in the reaction mixture in a yield of 63%-65% based on dG as the best source of guanine base. The yield of isolated ara-G was 48%-53%.


Assuntos
Arabinonucleosídeos/biossíntese , Escherichia coli/metabolismo , Arabinonucleosídeos/isolamento & purificação , Proteínas de Bactérias/metabolismo , Citarabina/metabolismo , Citosina Desaminase , Glutaral , Guanina/metabolismo , Nucleotídeos de Guanina/metabolismo , Nucleosídeo Desaminases/metabolismo , Purina-Núcleosídeo Fosforilase/metabolismo , Uridina Fosforilase/metabolismo
20.
Biochem Biophys Res Commun ; 167(1): 20-6, 1990 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-2155612

RESUMO

Analogs of 2-5A trimer 5'-monophosphate (2'-5')pA3,p5'A2'p5'A2'p5'A containing 9-(3-fluoro-3-deoxy-c-D-xylofuranosyl)adenine (AF) or 3'-fluoro-3'- deoxyadenosine (AF) at different positions of the chain have been synthesized. All of them were compared with (2'-5')pA3 and (2'-5')pA2 (3'dA) by (i) their ability to bind to 2-5A-dependent endoribonuclease(RNase L) of mouse L cells and of rabbit reticulocyte lysates and (ii) their susceptibility to the degradation by the (2'-5')phosphodiesterase activity. The results of this study suggest that the oligonucleotide conformation is important for its biochemical properties.


Assuntos
Nucleotídeos de Adenina/metabolismo , Endorribonucleases/metabolismo , Oligorribonucleotídeos/metabolismo , Diester Fosfórico Hidrolases/metabolismo , Animais , Células Cultivadas , Camundongos , Coelhos
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