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1.
Pharmacol Rep ; 74(4): 654-668, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35842893

RESUMO

BACKGROUND: The immunomodulatory properties of mesenchymal stem cells (MSCs) have made them a prospective treatment option for inflammatory and autoimmune disorders. Recent studies have found an association between the immunomodulatory function of MSCs and Toll-like receptors (TLRs). Here, we investigated the effect of priming with lipopolysaccharide (LPS) as TLR4 ligand or polyinosinic:polycytidylic acid (poly I:C) as TLR3 ligand on the immunomodulatory function of adipose-derived MSCs (ADMSCs) in vitro and for the first time in an adjuvant-induced arthritis model (AIA). METHODS: ADMSCs were treated with LPS or poly I:C for 1 h. Splenocyte proliferation in the presence of primed ADMSCs was assessed in vitro using an MTT assay. Next, we investigated the therapeutic effect of primed ADMSCs in vivo. Male Wistar rats were infused with complete Freund's adjuvant (CFA) to develop arthritis and then intraperitoneally treated with not-primed, poly I:C- or LPS-primed ADMSCs. Clinical signs, histopathological alteration, and serum and spleen cytokine levels were analyzed. RESULTS: Poly I:C-primed ADMSCs significantly reduced splenocytes proliferation, while ADMSCs primed with LPS increased splenocytes proliferation. Furthermore, poly I:C-primed ADMSCs significantly alleviated the clinical and histopathological severity and the secretion of inflammatory cytokines associated with Th17/Th1 such as IL-17 and IFN-γ. Poly I:C-primed ADMSCs also increased cytokines IL-10 and TGF-ß. TNF-α and IL-6 Levels were also markedly diminished in the serum of AIA animals treated with poly I:C-primed ADMSCs. In contrast, priming ADMSCs with LPS significantly reduced the therapeutic effect of ADMSCs in AIA animals. CONCLUSION: As a result of these findings, poly I:C priming may be a new technique for improving the therapeutic effects of MSCs in arthritic disorders.


Assuntos
Artrite Experimental , Transplante de Células-Tronco Mesenquimais , Células-Tronco Mesenquimais , Animais , Artrite Experimental/induzido quimicamente , Artrite Experimental/terapia , Citocinas , Ligantes , Lipopolissacarídeos/farmacologia , Masculino , Transplante de Células-Tronco Mesenquimais/métodos , Poli I-C/farmacologia , Ratos , Ratos Wistar
2.
Front Med ; 16(3): 358-377, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35687278

RESUMO

According to literature, certain microorganism productions mediate biological effects. However, their beneficial characteristics remain unclear. Nowadays, scientists concentrate on obtaining natural materials from live creatures as new sources to produce innovative smart biomaterials for increasing tissue reconstruction in tissue engineering and regenerative medicine. The present review aims to introduce microorganism-derived biological macromolecules, such as pullulan, alginate, dextran, curdlan, and hyaluronic acid, and their available sources for tissue engineering. Growing evidence indicates that these materials can be used as biological material in scaffolds to enhance regeneration in damaged tissues and contribute to cosmetic and dermatological applications. These natural-based materials are attractive in pharmaceutical, regenerative medicine, and biomedical applications. This study provides a detailed overview of natural-based biomaterials, their chemical and physical properties, and new directions for future research and therapeutic applications.


Assuntos
Engenharia Tecidual , Tecidos Suporte , Materiais Biocompatíveis/química , Humanos , Ácido Hialurônico , Medicina Regenerativa , Tecidos Suporte/química
3.
Carbohydr Polym ; 284: 119152, 2022 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-35287892

RESUMO

Fungal exopolysaccharides (EPSs) are natural biopolymers with diverse potential applications in the biomedical, packaging, cosmetic, and food industries. Fungal EPSs are easy to extract and purify polysaccharides that are biodegradable, biocompatible, with low immunogenicity, bioadhesion ability, antibacterial activity, and contain different reactive groups such as hydroxyl, carboxyl, and amine for chemical modifications. Despite fast progress in identifying and characterization fungal EPSs for biomedical applications, i.e., wound healing, drug, and gene delivery, only a few products have been commercialized based on fungal EPSs. This review critically discusses potential biomedical applications of fungi sourced EPSs in tissue engineering (TE), drug and gene delivery.


Assuntos
Quitosana , Antibacterianos/química , Antibacterianos/farmacologia , Quitosana/química , Fungos , Engenharia Tecidual , Cicatrização
4.
Mol Biomed ; 3(1): 6, 2022 Feb 17.
Artigo em Inglês | MEDLINE | ID: mdl-35174448

RESUMO

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) virus is a highly contagious microorganism, and despite substantial investigation, no progress has been achieved in treating post-COVID complications. However, the virus has made various mutations and has spread around the world. Researchers have tried different treatments to reduce the side effects of the COVID-19 symptoms. One of the most common and effective treatments now used is steroid therapy to reduce the complications of this disease. Long-term steroid therapy for chronic inflammation following COVID-19 is harmful and increases the risk of secondary infection, and effective treatment remains challenging owing to fibrosis and severe inflammation and infection. Sometimes our immune system can severely damage ourselves in disease. In the past, many researchers have conducted various studies on the immunomodulatory properties of stem cells. This property of stem cells led them to modulate the immune system of autoimmune diseases like diabetes, multiple sclerosis, and Parkinson's. Because of their immunomodulatory properties, stem cell-based therapy employing mesenchymal or hematopoietic stem cells may be a viable alternative treatment option in some patients. By priming the immune system and providing cytokines, chemokines, and growth factors, stem cells can be employed to build a long-term regenerative and protective response. This review addresses the latest trends and rapid progress in stem cell treatment for Acute Respiratory Distress Syndrome (ARDS) following COVID-19.

5.
Materials (Basel) ; 15(1)2022 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-35009464

RESUMO

The use of bioactive glasses (BGs) has been quite fruitful in hard tissue engineering due to the capability of these materials to bond to living bone. In this work, a melt-derived magnesium (Mg)-doped BG (composition: 45SiO2-3P2O5-26CaO-15Na2O-7MgO-4K2O (mol.%)) was synthesized for being used in bone reconstruction. The prepared BGs were then manufactured as three-dimensional (3D) scaffolds by using the sponge replica approach. The microstructure of the samples was assessed by X-ray diffraction (XRD) and the surface morphology was observed by using scanning electron microscopy (SEM). The in vitro bioactivity and the release of osteo-stimulatory Mg2+ ions from the prepared samples were investigated over 7 days of incubation in simulated body fluids (SBF). In vitro cellular analyses revealed the compatibility of the Mg-doped BGs with human osteosarcoma cells (MG-63 cell line). Moreover, the Mg-doped BGs could induce bone nodule formation in vitro and improve the migratory ability of human umbilical vein endothelial cells (HUVECs). In vivo osteogenic capacity was further evaluated by implanting the BG-derived scaffolds into surgically-created critical-size bone defects in rats. Histological and immunohistological observations revealed an appropriate bone regeneration in the animals receiving the glass-based scaffolds after 12 weeks of surgery. In conclusion, our study indicates the effectiveness of the Mg-doped BGs in stimulating osteogenesis in both in vitro and in vivo conditions.

6.
Materials (Basel) ; 14(14)2021 Jul 13.
Artigo em Inglês | MEDLINE | ID: mdl-34300827

RESUMO

For bone tissue engineering, stem cell-based therapy has become a promising option. Recently, cell transplantation supported by polymeric carriers has been increasingly evaluated. Herein, we encapsulated human olfactory ectomesenchymal stem cells (OE-MSC) in the collagen hydrogel system, and their osteogenic potential was assessed in vitro and in vivo conditions. Collagen type I was composed of four different concentrations of (4 mg/mL, 5 mg/mL, 6 mg/mL, 7 mg/mL). SDS-Page, FTIR, rheologic test, resazurin assay, live/dead assay, and SEM were used to characterize collagen hydrogels. OE-MSCs encapsulated in the optimum concentration of collagen hydrogel and transplanted in rat calvarial defects. The tissue samples were harvested after 4- and 8-weeks post-transplantation and assessed by optical imaging, micro CT, and H&E staining methods. The highest porosity and biocompatibility were confirmed in all scaffolds. The collagen hydrogel with 7 mg/mL concentration was presented as optimal mechanical properties close to the naïve bone. Furthermore, the same concentration illustrated high osteogenic differentiation confirmed by real-time PCR and alizarin red S methods. Bone healing has significantly occurred in defects treated with OE-MSCs encapsulated hydrogels in vivo. As a result, OE-MSCs with suitable carriers could be used as an appropriate cell source to address clinical bone complications.

7.
Polymers (Basel) ; 13(6)2021 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-33802198

RESUMO

Loss of skin integrity can lead to serious problems and even death. In this study, for the first time, the effect of exopolysaccharide (EPS) produced by cold-adapted yeast R. mucilaginosa sp. GUMS16 on a full-thickness wound in rats was evaluated. The GUMS16 strain's EPS was precipitated by adding cold ethanol and then lyophilized. Afterward, the EPS with polycaprolactone (PCL) and gelatin was fabricated into nanofibers with two single-needle and double-needle procedures. The rats' full-thickness wounds were treated with nanofibers and Hematoxylin and eosin (H&E) and Masson's Trichrome staining was done for studying the wound healing in rats. Obtained results from SEM, DLS, FTIR, and TGA showed that EPS has a carbohydrate chemical structure with an average diameter of 40 nm. Cell viability assessments showed that the 2% EPS loaded sample exhibits the highest cell activity. Moreover, in vivo implantation of nanofiber webs on the full-thickness wound on rat models displayed a faster healing rate when EPS was loaded into a nanofiber. These results suggest that the produced EPS can be used for skin tissue engineering applications.

8.
Mater Sci Eng C Mater Biol Appl ; 121: 111855, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33579488

RESUMO

Mechanical properties of tissue engineering nanofibrous scaffolds are of importance because they not only determine their ease of application, but also influence the environment for cell growth and proliferation. Cellulose nanocrystals (CNCs) are natural renewable nanoparticles that have been widely used for manipulating nanofibers' mechanical properties. In this article, cellulose nanoparticles were incorporated into poly(caprolactone) (PCL) solution, and composite nanofibers were produced. Ozawa-Flynn-Wall (OFW) methodology and X-ray diffraction were used to investigate the effect of CNC incorporation on PCL crystalline structure and its biological properties. Results showed that CNC incorporation up to 1% increases the crystallization activation energy and reduces the crystal volume, while these factors remain constant above this critical concentration. MTT assay and microscopic images of seeded cells on the nanofiber scaffolds indicated increased cell growth on the samples containing CNC. This behavior could be attributed to their greater hydrophilicity, which was confirmed using parallel exponential kinetics (PEK) model fitting to results obtained from dynamic vapor sorption (DVS) studies. Superior performance of CNC containing samples was also confirmed by in vivo implantation on full-thickness wounds. The wound area faded away more rapidly in these samples. H&E and Masson's trichrome staining showed better regeneration and more developed tissues in wounds treated with PCL-CNC1% nanofibers.


Assuntos
Nanofibras , Nanopartículas , Celulose , Cristalização , Cinética , Poliésteres , Engenharia Tecidual , Tecidos Suporte
9.
Curr Stem Cell Res Ther ; 16(6): 745-752, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33494682

RESUMO

The past decade has evidenced numerous developments in the treatment of heart diseases; however many patients with chronic heart failure suffer from low quality of life. Therapeutic methods, including drug-delivery as well as heart transplantation, have been used to improve quality of life. Cell therapy and tissue engineering have been recently introduced to the field of medicine as a novel therapeutic approach. Treatment of heart diseases has seen novel development through the introduction of cell therapy approaches. Based on the evidence, cell therapy has emerged as a promising therapeutic strategy for the treatment of cardiac diseases. Since the first cell transplant to patients, different types of (stem) cells have been studied. This study aims to provide a comprehensive review of different types of cells and their roles in cardiac cell-based therapy.


Assuntos
Miocárdio , Transplante de Células-Tronco , Engenharia Tecidual , Terapia Baseada em Transplante de Células e Tecidos , Coração , Humanos , Miócitos Cardíacos , Qualidade de Vida
10.
Int J Biol Macromol ; 166: 200-212, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-33190822

RESUMO

Nanotechnology-based fabricated wound dressings are known as appropriate substrates to enhance healing in both acute and chronic wounds. These types of materials have the ability to deliver therapeutic agents. In this study, a wound dressing including heparinized zinc oxide nanoparticles in combination with chitosan and poly(vinyl alcohol) was developed to investigate its antibacterial and regenerative properties in a rat model of full thickness skin wounds. By adding nanoparticles, the mechanical strength increased up to twice as compared to the sample without nanoparticles. In addition, heparin release profile follows the Hixson-Crowell release kinetic. Protein adsorption enhanced by adding nanoparticles in hydrogels and the prepared wound dressings were completely biocompatible. In terms of antibacterial activity, the minimum inhibitory concentration decreased by conjugation of heparin on the surface of zinc oxide nanoparticles compared to the non-functionalized nanoparticles, and, this shows the increased antibacterial synergistic effect by adding heparin to nanoparticles. Furthermore, it was found that the heparinized zinc oxide nanoparticles effectively accelerate wound closure, re-epithelialization and decrease collagen deposition compared to other groups after implantation. Hence, the prepared wound dressings have the capacity to significantly enhance healing of acute wounds.


Assuntos
Anti-Infecciosos/química , Curativos Hidrocoloides , Heparina/análogos & derivados , Hidrogéis/química , Nanopartículas Metálicas/química , Cicatrização/efeitos dos fármacos , Óxido de Zinco/química , Animais , Anti-Infecciosos/farmacologia , Linhagem Celular , Heparina/farmacologia , Hidrogéis/farmacologia , Masculino , Camundongos , Ratos , Ratos Wistar , Óxido de Zinco/farmacologia
11.
J Tissue Eng Regen Med ; 15(1): 78-87, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-33175464

RESUMO

Diaphragmatic wall defects caused by congenital disorders or disease remain a major challenge for physicians worldwide. Polymeric patches have been extensively explored within research laboratories and the clinic for soft tissue and diaphragm reconstruction. However, patch usage may be associated with allergic reaction, infection, granulation, and recurrence of the hernia. In this study, we designed and fabricated a porous scaffold using a combination of 3D printing and freeze-drying techniques. A 3D printed polycaprolactone (PCL) mesh was used to reinforcegelatin scaffolds, representing an advantage over previously reported examples since it provides mechanical strength and flexibility. In vitro studies showed that adherent cells were anchorage-dependent and grew as a monolayer attached to the scaffolds. Microscopic observations indicated better cell attachments for the scaffolds with higher gelatin content as compared with the PCL control samples. Tensile testing demonstrated the mechanical strength of samples was significantly greater than adult diaphragm tissue. The biocompatibility of the specimens was investigated in vivo using a subcutaneous implantation method in Bagg albino adult mice for 20 days, with the results indicating superior cellular behavior and attachment on scaffolds containing gelatin in comparison to pure PCL scaffolds, suggesting that the porous PCL/gelatin scaffolds have potential as biodegradable and flexible constructs for diaphragm reconstruction.


Assuntos
Diafragma , Gelatina , Poliésteres , Impressão Tridimensional , Procedimentos Cirúrgicos Reconstrutivos , Tecidos Suporte/química , Células 3T3 , Animais , Diafragma/metabolismo , Diafragma/cirurgia , Liofilização , Gelatina/química , Gelatina/farmacologia , Camundongos , Poliésteres/química , Poliésteres/farmacologia , Porosidade
12.
J Mater Sci Mater Med ; 31(11): 101, 2020 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-33140201

RESUMO

To boost the healing process in a full-thickness wound, a simple and efficient strategy based on adipose-derived mesenchymal stem cells (ADSCs) transplantation is described in this work. To increase the chance of ADSCs immobilization in the wound bed and prevent its migration, these cells are fully grown on the surface of a thermoresponsive dressing membrane under in vitro condition. Then, the cells sheet with their secreted extracellular matrix (ECM) is transferred to the damaged skin with the help of this dressing membrane. This membrane remains on wound bed and acts both as a cell sheet transfer vehicle, after external reduction of temperature, and protect wound during the healing process like a common wound dressing. The visual inspection of wounded skin (rat animal model) at selected time intervals shows a higher wound closure rate for ADSCs treated group. For this group of rats, the better quality of reconstructed tissue is approved by results of histological and immunohistochemical analysis since the higher length of the new epidermis, the higher thickness of re-epithelialization layer, a higher level of neovascularization and capillary density, and the least collagen deposition are detected in the healed tissue.


Assuntos
Adipócitos/citologia , Epiderme/metabolismo , Células-Tronco Mesenquimais/citologia , Engenharia Tecidual/instrumentação , Tecido Adiposo/citologia , Animais , Bandagens , Adesão Celular , Proliferação de Células , Matriz Extracelular/metabolismo , Imuno-Histoquímica , Masculino , Ratos , Ratos Wistar , Células-Tronco/citologia , Temperatura , Engenharia Tecidual/métodos , Cicatrização
13.
Biotechnol J ; 15(12): e2000117, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32845071

RESUMO

Quantum dots (QDs) are semiconductor materials that have gained great interest due to their unique characteristics like optical properties. They are extensively being used in different areas, including solar cells, light-emitting diodes, laser technology, as well as biological and biomedical applications. In this review, comprehensive information about different aspects of QDs is provided, including their types and classifications, synthesis approaches, in vitro and in vivo toxicity, biological applications, and potentials in clinical applications. With a focus on the biological aspects, the respective in vitro and in vivo studies are collected and presented. Various surface modifications on QDs are discussed as directly influencing their properties like toxicity and optical abilities. Given the promising results, these materials are clinically used for targeted molecular therapy and imaging. However, there are a large number of questions that should be addressed before the wide application of QDs in a clinical setting. Regarding the existing barriers to QDs, suggestions are given and discussed to present an appropriate route for the clinical use of these materials.


Assuntos
Pontos Quânticos , Diagnóstico por Imagem , Luz
14.
Heliyon ; 6(4): e03798, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32368647

RESUMO

There have been several attempts to design innovative biomaterials as surface coatings to enhance the biological performance of biomedical implants. The objective of this study was to design multifunctional Cu/a-C:H thin coating depositing on the Ti-6Al-4V alloy (TC4) via magnetron sputtering in the presence of Ar and CH4 for applications in bone implants. Moreover, the impact of Cu amount and sp2/sp3 ratio on the interior stress, corrosion behavior, mechanical properties, and tribological performance and biocompatibility of the resulting biomaterial was discussed. X-ray photoelectron spectroscopy (XPS) revealed that the sp2/sp3 portion of the coating was enhanced for samples having higher Cu contents. The intensity of the interior stress of the Cu/a-C:H thin bio-films decreased by increase of Cu content as well as the sp2/sp3 ratio. By contrast, the values of Young's modulus, the H3/E2 ratio, and hardness exhibited no significant difference with enhancing Cu content and sp2/sp3 ratio. However, there was an optimum Cu content (36.8 wt.%) and sp2/sp3 ratio (4.7) that it is feasible to get Cu/a-C:H coating with higher hardness and tribological properties. Electrochemical impedance spectroscopy test results depicted significant improvement of Ti-6Al-4V alloy corrosion resistance by deposition of Cu/a-C:H thin coating at an optimum Ar/CH4 ratio. Furthermore, Cu/a-C:H thin coating with higher Cu contents showed better antibacterial properties and higher angiogenesis and osteogenesis activities. The coated samples inhibited the growth of bacteria as compared to the uncoated sample (p < 0.05). In addition, such coating composition can stimulate angiogenesis, osteogenesis and control host response, thereby increasing the success rate of implants. Moreover, Cu/a-C:H thin films encouraged development of blood vessels on the surface of titanium alloy when the density of grown blood vessels was increased with enhancing the Cu amount of the films. It is speculated that such coating can be a promising candidate for enhancing the osseointegration features.

15.
Methods ; 171: 11-19, 2020 01 15.
Artigo em Inglês | MEDLINE | ID: mdl-31326597

RESUMO

The efficacy of decellularized products for healing of acute and chronic wounds mostly relies on physical and chemical properties, processing methods and host response. Human Amniotic Membrane (HAM) is considered as an effective and highly used wound dressing in clinic. According to the proposed decellularization protocols for developing of HAM, we have compared different protocols to introduce the most efficient methods, which can be used as a functional dermal matrix. In this study, different methods of HAM decellularization were used to achieve an optimal process. After achievement of appropriate decellularized method in vitro the amniotic membrane were examined in term of animal in vivo study and human clinical trial. The results of in vitro and in vivo assay indicate that the HAMs which were prepared with peracetic acid (2 M) had a significantly different in term of GAGs quantification, DNA isolation and quantification, histological assessment, collagen analysis, Cell-Tissue Interaction Study and cytotoxicity (P < 0/05). Tissue samples treated with peracetic acid (2 M) were more acceptable than that of samples prepared with other protocols in terms of preserving natural components and structure and removing of cell fragments. The peracetic acid-processed HAM was further functionally evaluated through in vivo assessments that can further lead to tissue reconstruction within the human host.


Assuntos
Derme Acelular , Âmnio/química , Bandagens , Cicatrização/efeitos dos fármacos , Âmnio/citologia , Animais , Ensaios Clínicos como Assunto , Colágeno/química , Colágeno/farmacologia , Matriz Extracelular/química , Humanos , Modelos Animais , Engenharia Tecidual , Tecidos Suporte/química
16.
ACS Biomater Sci Eng ; 6(11): 6285-6298, 2020 11 09.
Artigo em Inglês | MEDLINE | ID: mdl-33449643

RESUMO

The human amniotic membrane (HAM) has been viewed as a potential regenerative material for a wide variety of injured tissues because of its collagen-rich content. High degradability of HAM limits its wide practical application in bone tissue engineering. In this study, the natural matrix of the decellularized amniotic membrane was developed by the double diffusion method. The results confirmed a reduction of the amniotic membrane's degradability because of the deposition of calcium and phosphate ions during the double diffusion process. Real-time PCR results showed a high expression of osteogenesis-related genes from adipose-derived mesenchymal stem cells (ADMSCs) cultured on the surface of the developed mineralized amniotic membrane (MAM). Further in vivo experiments were conducted using an MAM preseeded with ADMSCs and a critical-size rat calvarial defect model. Histopathological results confirmed that the MAM + cell sample has excellent potential in bone regeneration.


Assuntos
Âmnio , Engenharia Tecidual , Animais , Biomimética , Regeneração Óssea , Diferenciação Celular , Humanos , Ratos
17.
J Cell Physiol ; 234(12): 23763-23773, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31173364

RESUMO

Olfactory ectomesenchymal stem cells (OE-MSCs) possess the immunosuppressive activity and regeneration capacity and hold a lot of promises for neurodegenerative disorders treatment. This study aimed to determine OE-MSCs which are able to augment and differentiate into functional neurons and regenerate the CNS and also examine whether the implantation of OE-MSCs in the pars compacta of the substantia nigra (SNpc) can improve Parkinson's symptoms in a rat model-induced with 6-hydroxydopamine. We isolated OE-MSCs from lamina propria in olfactory mucosa and characterized them using flow cytometry and immunocytochemistry. The therapeutic potential of OE-MSCs was evaluated by the transplantation of isolated cells using a rat model of acute SN injury as a Parkinson's disease. Significant behavioral improvement in Parkinsonian rats was elicited by the OE-MSCs. The results demonstrate that the expression of PAX2, PAX5, PITX3, dopamine transporter, and tyrosine hydroxylase was increased by OE-MSCs compared to the control group which is analyzed with real-time polymerase chain reaction technique and immunohistochemical staining. In the outcome, the transplantation of 1,1'-dioctadecyl-3,3,3'3'-tetramethyl indocarbocyanine perchlorate labeled OE-MSCs that were fully differentiated to dopaminergic neurons contribute to a substantial improvement in patients with Parkinson's. Together, our results provide that using OE-MSCs in neurodegenerative disorders might lead to better neural regeneration.


Assuntos
Neurônios Dopaminérgicos/citologia , Transplante de Células-Tronco Mesenquimais , Células-Tronco Mesenquimais/citologia , Mucosa Olfatória/citologia , Doença de Parkinson/terapia , Animais , Terapia Baseada em Transplante de Células e Tecidos/métodos , Células Cultivadas , Proteínas da Membrana Plasmática de Transporte de Dopamina/biossíntese , Proteínas de Homeodomínio/biossíntese , Masculino , Células-Tronco Mesenquimais/metabolismo , Fator de Transcrição PAX2/biossíntese , Fator de Transcrição PAX5/biossíntese , Ratos , Ratos Wistar , Fatores de Transcrição/biossíntese , Tirosina 3-Mono-Oxigenase/biossíntese
18.
Recent Pat Biotechnol ; 13(2): 137-148, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30973107

RESUMO

BACKGROUND: The increase of oxidant compounds is the most well-known reasons for the tolerance to the analgesic properties of Morphine. Additionally, the production of proxy-nitrite impairs receptors, proteins and enzymes involved in the signaling pathways of analgesia, apoptosis and necrosis. Also, we revised all patents relating to opioid tolerance control methods. OBJECTIVE: The aim of this study was to assess the effects of Alpha-tocopherol as an anti-oxidant agent to reduce Morphine tolerance. METHOD: Forty male rats randomly divided into four groups. 10 mg/kg of morphine was injected subcutaneously to create the desired level of tolerance. After modeling, 70 mg/kg Alpha- Tocopherol was injected intraperitoneal. Also, the hot plate recorded pain threshold alterations was used to evaluate the behavioral test. All tissue samples were extracted from the spinal cord, thalamus and frontal cortex for molecular and gene expression evaluations. Also, the effect of Alpha- Tocopherol on the apoptosis and necrosis parameters was analyzed using nissl staining and tunel test. RESULTS: The time latency results showed that there were no significant differences in the different days in groups treated with Morphine plus Alpha-Tocopherol. However, our data highlighted that the pain threshold and their time latency in respond to it had substantially increased in comparison with the control group. Furthermore, we found that the Alpha-Tocopherol obviously decreased c-fos gene expression, especially in the spinal cord. CONCLUSION: Thus, co-administration of Alpha-Tocopherol with Morphine can decrease the adverse effects of nitrite proxy, which is released due to repeated injections of Morphine.


Assuntos
Analgésicos Opioides/farmacologia , Antioxidantes/farmacologia , Tolerância a Medicamentos/genética , Genes fos , Morfina/farmacologia , Dor/tratamento farmacológico , alfa-Tocoferol/farmacologia , Animais , Lobo Frontal/efeitos dos fármacos , Lobo Frontal/metabolismo , Expressão Gênica/efeitos dos fármacos , Injeções Intraperitoneais , Injeções Subcutâneas , Masculino , Dor/genética , Dor/metabolismo , Dor/fisiopatologia , Patentes como Assunto , Ratos , Medula Espinal/efeitos dos fármacos , Medula Espinal/metabolismo , Tálamo/efeitos dos fármacos , Tálamo/metabolismo
19.
Mol Neurobiol ; 56(1): 307-318, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29549645

RESUMO

A neurological disorder is any disorder or abnormality in the nervous system. Among different neurological disorders, Alzheimer's disease (AD) is recognized as the sixth leading cause of death globally. Considerable research has been conducted to find pioneer treatments for this devastating disorder among which cell therapy has attracted remarkable attentions over the last decade. Up to now, targeted differentiation into specific desirable cell types has remained a major obstacle to clinical application of cell therapy. Also, potential risks including uncontrolled growth of stem cells could be disastrous. In our novel protocol, we used basal forebrain cholinergic progenitor cells (BFCN) derived from human chorion-derived mesenchymal stem cells (hC-MSCs) which made it possible to obtain high-quality population of cholinergic neurons and in vivo in much shorter time period than previous established methods. Remarkably, the transplanted progenitors fully differentiated to cholinergic neurons which in turn integrated in higher cortical networks of host brains, resulting in significant improvement in cognitive assessments. This method may have profound implications in cell therapies for any other neurodegenerative disorders. Graphical Abstract ᅟ.


Assuntos
Neurônios Colinérgicos/transplante , Córion/citologia , Doenças do Sistema Nervoso/terapia , Transplante de Células-Tronco , Células-Tronco/citologia , Doença de Alzheimer/patologia , Doença de Alzheimer/terapia , Animais , Diferenciação Celular , Neurônios Colinérgicos/citologia , Cognição , Modelos Animais de Doenças , Humanos , Masculino , Prosencéfalo/citologia , Ratos Wistar , Recuperação de Função Fisiológica
20.
Mol Neurobiol ; 56(1): 319-320, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29700773

RESUMO

The original version of this article unfortunately contained mistake in the affiliation. Affiliation 1 should be read as "Neuroscience Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran". The original article has been corrected.

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