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1.
Phys Rev Lett ; 123(1): 015701, 2019 Jul 03.
Artigo em Inglês | MEDLINE | ID: mdl-31386397

RESUMO

Strain glass is being established as a conceptually new state of matter in highly doped alloys, yet the understanding of its microscopic formation mechanism remains elusive. Here, we use a combined numerical and experimental approach to establish, for the first time, that the formation of strain glasses actually proceeds via the gradual percolation of strain clusters, namely, localized strain clusters that expand to reach the percolating state. Furthermore, our simulation studies of a wide variety of specific materials systems unambiguously reveal the existence of distinct scaling properties and universal behavior in the physical observables characterizing the glass transition, as obeyed by many existing experimental findings. The present work effectively enriches our understanding of the underlying physical principles governing glassy disordered materials.

2.
Yi Chuan ; 41(4): 318-326, 2019 Apr 20.
Artigo em Chinês | MEDLINE | ID: mdl-30992253

RESUMO

Breast cancer is one of the most common malignant tumors endangering women. It has been found that the subunits of the COP9 complex are closely related to the occurrence and development of malignant tumors, and the CSN4 subunit plays an important role in regulating the whole complex. In the breast cancer cell line MDA-MB-231, we successfully established a lentivirus-mediated CSN4-knockdown cell line. CCK8 cell proliferation assays and colony formation experiments confirmed that CSN4 knockdown significantly decreased the cellular proliferation rate. Cell cycle analysis showed that CSN4 knockdown increased sub-G1 population and induced apoptosis. In addition, Western blotting assays confirmed that CSN4 regulates the expression of CDK6 and Caspase3, suggesting that CSN4 modulates the proliferation and apoptosis of breast cancer cells by regulating the expression of CDK6 and Caspase3 genes and thereby tumorigenesis. This study has deepened our understanding of the molecular mechanism of apoptosis and cell growth in breast cancers, and further revealed the role and mechanism of CSN4 in cancer biology.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal/genética , Apoptose , Neoplasias da Mama/genética , Neoplasias da Mama/patologia , Complexo do Signalossomo COP9/genética , Proliferação de Células , Caspase 3/metabolismo , Linhagem Celular Tumoral , Quinase 6 Dependente de Ciclina/metabolismo , Feminino , Regulação Neoplásica da Expressão Gênica , Técnicas de Silenciamento de Genes , Humanos
3.
Adv Mater ; : e1802902, 2018 Aug 14.
Artigo em Inglês | MEDLINE | ID: mdl-30109765

RESUMO

The voltage modulation of yttrium iron garnet (YIG) is of practical and theoretical significance; due to its advantages of compactness, high-speed response, and energy efficiency, it can be used for various spintronic applications, including spin-Hall, spin-pumping, and spin-Seebeck effects. In this study, a significant ferromagnetic resonance change is achieved within the YIG/Pt bilayer heterostructures uisng ionic modulation, which is accomplished by modifying the interfacial magnetism in the deposited "capping" platinum layer. With a small voltage bias of 4.5 V, a large ferromagnetic field shift of 690 Oe is achieved in heterostructures of YIG (13 nm)/Pt (3 nm)/(ionic liquid, IL)/(Au capacitor). The remarkable magnetoelectric (ME) tunability comes from the additional and voltage-induced ferromagnetic ordering, caused by uncompensated d-orbital electrons in the Pt metal layer. Confirmed by first-principle calculations, this finding paves the way for novel voltage-tunable YIG-based spintronics.

4.
Adv Mater ; 30(39): e1803612, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30133018

RESUMO

E-field control of antiferromagnetic (AFM) orders is promising for the realization of fast, compact, and energy-efficient AFM applications. However, as the AFM spins are strongly pinned, the E-field control process is mainly based on the exchange bias regulation that usually confines at a low temperature. Here, a new magnetoelectric (ME) coupling mechanism for the modulation of AFM orders at room temperature is explored. Based on the FeCoB/Ru/FeCoB/(011) Pb(Mg1/3 Nb2/3 )O3 -PbTiO3 (PMN-PT) synthetic antiferromagnetic (SAF) heterostructures, the external E-field generates relative magnetization switching in the two ferromagnetic (FM) layers, leading the Ruderman-Kittel-Kasuya-Yosida (RKKY) interaction tuning. This voltage-induced switching behavior can be repeated in a stable and reversible manner for various SAFs, which is a key challenge in the E-field control of AFM coupling and is not resolved yet. The voltage-induced RKKY interaction changes by analyzing the dynamic optical and acoustic modes is quantified, and with first-principles calculations, it is found that the distortion of the Fermi surface by the lattice reconstruction is the key of the relative magnetization switching and RKKY interaction modulation. This voltage control of the RKKY interaction in ME heterostructures provides an easy way to achieve the next generation of AFM/FM spintronic applications.

5.
Adv Mater ; 30(30): e1801639, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-29809283

RESUMO

Electric field (E-field) modulation of perpendicular magnetic anisotropy (PMA) switching, in an energy-efficient manner, is of great potential to realize magnetoelectric (ME) memories and other ME devices. Voltage control of the spin-reorientation transition (SRT) that allows the magnetic moment rotating between the out-of-plane and the in-plane direction is thereby crucial. In this work, a remarkable magnetic anisotropy field change up to 1572 Oe is achieved under a small operation voltage of 4 V through ionic liquid (IL) gating control of SRT in Au/[DEME]+ [TFSI]- /Pt/(Co/Pt)2 /Ta capacitor heterostructures at room temperature, corresponding to a large ME coefficient of 378 Oe V-1 . As revealed by both ferromagnetic resonance measurements and magnetic domain evolution observation, the magnetization can be switched stably and reversibly between the out-of-plane and in-plane directions via IL gating. The key mechanism, revealed by the first-principles calculation, is that the IL gating process influences the interfacial spin-orbital coupling as well as net Rashba magnetic field between the Co and Pt layers, resulting in the modulation of the SRT and in-plane/out-of-plane magnetization switching. This work demonstrates a unique IL-gated PMA with large ME tunability and paves a way toward IL gating spintronic/electronic devices such as voltage tunable PMA memories.

6.
Nat Commun ; 9(1): 991, 2018 03 07.
Artigo em Inglês | MEDLINE | ID: mdl-29515180

RESUMO

To overcome the fundamental challenge of the weak natural response of antiferromagnetic materials under a magnetic field, voltage manipulation of antiferromagnetic interaction is developed to realize ultrafast, high-density, and power efficient antiferromagnetic spintronics. Here, we report a low voltage modulation of Ruderman-Kittel-Kasuya-Yosida (RKKY) interaction via ionic liquid gating in synthetic antiferromagnetic multilayers of FeCoB/Ru/FeCoB and (Pt/Co)2/Ru/(Co/Pt)2. At room temperature, the distinct voltage control of transition between antiferromagnetic and ferromagnetic ordering is realized and up to 80% of perpendicular magnetic moments manage to switch with a small-applied voltage bias of 2.5 V. We related this ionic liquid gating-induced RKKY interaction modification to the disturbance of itinerant electrons inside synthetic antiferromagnetic heterostructure and the corresponding change of its Fermi level. Voltage tuning of RKKY interaction may enable the next generation of switchable spintronics between antiferromagnetic and ferromagnetic modes with both fundamental and practical perspectives.

7.
Pediatr Cardiol ; 39(5): 911-923, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29556784

RESUMO

Normal ECG values in newborns, infants, and children have been collected and published. ECG in the adolescent, however, remains, to be collected and studied. The present study was designed and carried out to establish the normal ECG standards in male and female adolescents. A total of 898 school children and adolescents screened and examined as healthy were divided by age and sex into 6-9, 9-13, and 13-18 years age-groups. A 12 lead conventional ECG was recorded in 10 mm/mV and 25 mm/s, utilizing an automated Fukuda Denshi FCP-4301, MS-DOS/IBM-AT ECG machine. Lead V3R was not taken. Analog-to-digital conversion was performed by Fukuda signal acquisition module at a sampling rate of 500 Hz. The data on 69 ECG parameters were analyzed for the mean, standard deviation, 2nd to 98th percentiles, 95% confidence intervals, and sex difference. Normal values on 69 ECG parameters, sex-specific heart rate, P-QRS-T interval, duration, axis, wave amplitude, and calculated R/S amplitude ratio and ventricular activation time by age-group and sex were established. Male and female difference was noted in 49 (71.0%) parameters, of which 3 (6.1%) began in 6-9 years age-group, 30 (61.2%) began in 9-13 years age-group, and 16 (32.7%) in 13-18 years age-group. No sex difference occurred in 20 (29.0%) parameters. Normal male and female ECG standards on 69 ECG parameters in the adolescent were established. ECG sex difference began to appear the earliest at ages 6-9 years, and it occurred mostly at ages 9-13 years and 13-18 years, reflecting the anatomical and physiological consequences of puberty.


Assuntos
Eletrocardiografia/métodos , Frequência Cardíaca/fisiologia , Adolescente , Fatores Etários , Criança , Estudos Transversais , Feminino , Humanos , Masculino , Valores de Referência , Fatores Sexuais , Maturidade Sexual
8.
Phys Chem Chem Phys ; 20(1): 553-561, 2017 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-29220056

RESUMO

Ferromagnetic (FM) two-dimensional (2D) transition metal dichalcogenides (TMDs) have potential applications in modern electronics and spintronics and doping of TMDs with transition metals can enhance the magnetic characteristics. In this work, the structural stability, electronic states, and magnetic properties of Mn-doped monolayer/bilayer MoS2 are studied systematically by first-principles calculations. Substitutional Mn dopants at the Mo sites are energetically favorable in both monolayer and bilayer MoS2 under the S-rich condition which is common in the synthesis of MoS2 nanosheets. Two Mn dopants participate in the FM interaction in monolayer MoS2 and magnetic coupling of two Mn dopants via the double-exchange mechanism can be mediated by the nearest neighboring S. Magnetic coupling can be ascribed to the competition between the double-exchange, direct-exchange, and super-exchange interactions, which take place between two Mn dopants in bilayer MoS2 with the MniMnMo, MniMnS and MnMo-MnMo configurations. Our results reveal the geometrical dependence of magnetic-exchange coupling suggesting that Mn-doped monolayer/bilayer MoS2 has large potential in spintronic devices.

9.
Sci Rep ; 7(1): 15166, 2017 11 09.
Artigo em Inglês | MEDLINE | ID: mdl-29123129

RESUMO

In van der Waals epitaxial growth, the substrate plays a particularly important role in the crystal morphology. Here, we synthesized MoS2 by chemical vapour deposition on silicon carbide (SiC). The obtained MoS2 dendritic crystals show six-fold symmetry, which are different from the conventional triangular shapes on SiO2 substrate and from those with three-fold symmetry on SrTiO3 substrate. Interestingly, these MoS2 dendritic crystals on SiC exhibit an average fractal dimension 1.76, which is slightly larger than the classical Diffusion-limited-Aggregation fractal dimension 1.66. The first principle calculation indicates that the six-fold symmetry of the dendritic MoS2 is determined by the lattice symmetry of SiC. To further demonstrating the substrate effect, we break the natural six-fold lattice symmetry of SiC (0001) into groove arrays through etching the substrate. And then we successfully synthesized cross-type dendritic crystal MoS2 with two-fold symmetry. Its average fractal dimension 1.83 is slightly larger than the fractal dimension 1.76 of the previous MoS2 dendrite with six-fold symmetry. In a word, the symmetry of SiC substrate determined the symmetry and the fractal dimension of the dendritic MoS2. This work provides one possibility of inducing the growth orientation of dendritic crystals through controlling the substrate surface symmetry artificially.

10.
J Pediatr ; 180: 80-86.e2, 2017 01.
Artigo em Inglês | MEDLINE | ID: mdl-27817879

RESUMO

OBJECTIVE: To investigate the postnatal risk of Kawasaki disease and coronary complications from a nationwide birth cohort in Taiwan, a country with the third-highest incidence of Kawasaki disease worldwide. STUDY DESIGN: We enrolled children born between 2000 and 2009 with complete postnatal medical care records for 2000-2014 in the Taiwan national database. RESULTS: Out of a total of 2 150 590 live births, we identified 6690 (62.6% boys) patients with Kawasaki disease. The onset was mostly (93.9%) within the first 5 years of life (median, 16 months; 38% during infancy), but was rare within the first 3 months of life. The overall cumulative incidence of Kawasaki disease by age 5 years was 2.78‰ (3.33‰ for boys and 2.17‰ for girls; P < .001) and exhibited an increasing trend with birth year (from 2.28‰ for 2000 to 3.67‰ for 2009). The incidence ratio was 1.535 in boys and 1.055 in each increasing year. Kawasaki disease recurred more often in younger patients (cumulative incidence, 2.3% in infants vs 1.7% in children aged 1-4 years). Coronary complications occurred in 16.2% of the patients, including 4 cases of acute myocardial infarction (3 occuring during the acute stage and 1 occurring 5 years later). The probability of a major cardiac event (infarction, undergoing percutaneous coronary intervention or coronary artery bypass grafting, or death) by adolescence was 1.9%. CONCLUSIONS: The postnatal risk of Kawasaki disease was 3‰-4‰ and increased with every birth year. Patients with Kawasaki disease are at substantial risk for a major cardiac events during childhood.


Assuntos
Síndrome de Linfonodos Mucocutâneos/epidemiologia , Pré-Escolar , Estudos de Coortes , Doença das Coronárias/epidemiologia , Doença das Coronárias/etiologia , Bases de Dados Factuais , Feminino , Humanos , Incidência , Lactente , Masculino , Síndrome de Linfonodos Mucocutâneos/complicações , Medição de Risco , Taiwan
11.
Acta Cardiol Sin ; 31(6): 500-6, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-27122914

RESUMO

UNLABELLED: With advances that have been made over the recent decades in transcatheter and surgical interventions, most patients with congenital heart disease (CHD) can survive into adulthood. Overall, probably half of these surviving patients are female. When these female CHD patients reach childbearing age, however, pregnancy management will be a major issue. In order to meet the demands of fetal growth, the maternal cardiovascular system starts a series of adaptations beginning in early pregnancy. These adaptations include: decreased systemic and pulmonary vascular resistances, decreased blood pressure, expansion of the blood volume, increased heart rate and increased cardiac output. For women with CHD, this hemodynamic alteration may increase the risks of adverse cardiovascular events as well as the fetal and neonatal complications. Therefore, proper risk stratification and effective counseling for women with CHD who are planning their pregnancies is an important undertaking. KEY WORDS: Congenital heart disease; Pregnancy.

12.
J Pediatr ; 163(1): 126-31.e1, 2013 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-23312687

RESUMO

OBJECTIVE: To update the epidemiologic trend in Kawasaki disease (KD) and develop models for projection. STUDY DESIGN: From our national databases 2000-2010 and previous studies, we obtained the epidemiologic data to develop and validate system dynamics models. Population model incorporated birth rate and mortality. KD model incorporated the population at risk, incidence, and risk of coronary complications. RESULTS: The average annual incidence in age group <5, 5-10, 10-15, and 15-20 years was 67.3, 5.75, 0.79, and 0.26 per 100,000. The KD population was 23,349 and the model estimated 20,254 patients with KD, and 25% of these patients received medical care or continued surveillance in 2010. Projection up to 2030 suggests an average of 725 new patients with KD annually and a KD population of 35,006 by 2030. In 2030, 1469 patients with KD will need medical care for coronary complications. Simulation on the model modified to US data is also effective and suggests an average of 6200 new patients annually and KD population of 161,776 by 2030, and 5664 patients will need coronary care in 2030. By 2030, there will be 1 per 700 people in Taiwan and 1 per 1600 in the US with a history of KD. CONCLUSION: Simulations on our system dynamics models tailored to any epidemiologic and outcome variables and any changes with medical advance can dynamically project the futures.


Assuntos
Síndrome de Linfonodos Mucocutâneos/epidemiologia , Adolescente , Adulto , Criança , Pré-Escolar , Bases de Dados Factuais , Previsões , Humanos , Lactente , Modelos Estatísticos , Prevalência , Taiwan/epidemiologia , Adulto Jovem
13.
Sci Total Environ ; 429: 266-71, 2012 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-22578525

RESUMO

The oxy-fuel combustion system is a promising technology to control CO2 and NO(x) emissions. Furthermore, sulfation reaction mechanism under CO2-rich atmospheric condition in a furnace may lead to in-furnace desulfurization. In the present study, we evaluated characteristics of calcium carbonate (CaCO3) sorbent particles under different atmospheric conditions. To examine the physical/chemical characteristics of CaCO3, which is used as a sorbent particle for in-furnace desulfurization in the oxy-fuel combustion system, they were injected into high temperature drop tube furnace (DTF). Experiments were conducted at varying temperatures, residence times, and atmospheric conditions in a reactor. To evaluate the aerosolizing characteristics of the CaCO3 sorbent particle, changes in the size distribution and total particle concentration between the DTF inlet and outlet were measured. Structural changes (e.g., porosity, grain size, and morphology) of the calcined sorbent particles were estimated by BET/BJH, XRD, and SEM analyses. It was shown that sorbent particles rapidly calcined and sintered in the air atmosphere, whereas calcination was delayed in the CO2 atmosphere due to the higher CO2 partial pressure. Instead, the sintering effect was dominant in the CO2 atmosphere early in the reaction. Based on the SEM images, it was shown that the reactions of sorbent particles could be explained as a grain-subgrain structure model in both the air and CO2 atmospheres.

14.
Circ Cardiovasc Qual Outcomes ; 5(4): 566-70, 2012 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-22589296

RESUMO

BACKGROUND: Patients with Kawasaki disease (kDa) may develop coronary arterial lesions and subsequent coronary events. The first reported case in Taiwan was in 1976, and the annual incidence from 2003 to 2006 was 69/100 000 children < 5 years. A population study from Taiwan, a country with a high incidence of kDa, national health insurance, and easily accessible medical care, would adequately reflect the long-term risk. METHODS AND RESULTS: We retrieved the data of kDa patients from a national health insurance 2000 to 2010 database of Taiwan, a country with a child health index similar to those in the United States. The occurrence of coronary complications and interventions was identified by the respective International Classification of Diseases, Ninth Revision, codes. The prevalence of kDa in the population < 40 years was 34.9/100 000 (male/female ratio, 1.47). Coronary complications occurred in 1254 patients (5.37%; male/female ratio, 2.19), with an average annual risk of 2.4% (2.7% for males and 2.0% for females). An acute myocardial infarction occurred in 19 patients (0.08%; 18 males and 1 female), of whom one third were aged between 10 and 15 years (median, 15.7 years; range, 0.7-36.7 years). A coronary intervention was performed by catheterization in 18 patients (all males) at a median age of 24.5 years and by surgery in 10 patients (male/female ratio, 4.0) at a median age of 21.7 years, with mortality at discharge being 0% and 25%, respectively. CONCLUSIONS: This study estimated the overall prevalence of kDa (≈1/2940) in a population < 40 years. They, particularly the males, carry long-term coronary risks from a young age. Risk stratification for a timely coronary intervention and risk modification are mandatory.


Assuntos
Doença das Coronárias/epidemiologia , Síndrome de Linfonodos Mucocutâneos/epidemiologia , Adolescente , Adulto , Fatores Etários , Angioplastia Coronária com Balão/efeitos adversos , Angioplastia Coronária com Balão/mortalidade , Cateterismo Cardíaco/efeitos adversos , Cateterismo Cardíaco/mortalidade , Criança , Pré-Escolar , Ponte de Artéria Coronária/efeitos adversos , Ponte de Artéria Coronária/mortalidade , Doença das Coronárias/mortalidade , Doença das Coronárias/terapia , Bases de Dados Factuais , Feminino , Humanos , Lactente , Masculino , Síndrome de Linfonodos Mucocutâneos/mortalidade , Infarto do Miocárdio/epidemiologia , Isquemia Miocárdica/epidemiologia , Programas Nacionais de Saúde , Prevalência , Medição de Risco , Fatores de Risco , Fatores Sexuais , Taiwan/epidemiologia , Fatores de Tempo , Resultado do Tratamento , Adulto Jovem
15.
Bioresour Technol ; 101(11): 4187-92, 2010 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-20144863

RESUMO

A simple expression for the apparent reaction rate of large wood char gasification with steam is proposed. Large char samples were gasified under steam atmosphere using a thermo-balance reactor. The apparent reaction rate was expressed as the product of the intrinsic rate and the effective factor. The effective factor was modified to include the effect of change in char diameter and intrinsic reaction rate during the reaction. Assuming uniform conversion ratio throughout a particle, the simplified reaction scheme was divided into three stages. In the initial stage, the local conversion ratio increases without particle shrinkage. In the middle stage, the particle shrinks following the shrinking core model without change in the local conversion ratio. In the final stage, the local conversion ratio increases without particle shrinkage. The validity of the modified effective value was confirmed by comparison with experimental results.


Assuntos
Gases , Vapor , Madeira , Cinética
16.
Artigo em Chinês | MEDLINE | ID: mdl-18441993

RESUMO

OBJECTIVE: To prepare and identify immune serum against the recombinant fusion protein of rhoptry 2 (ROP2) and major surface protein 1(P30) from Toxoplasma gondii. METHODS: The constructed recombinant plasmid of pET28b/ROP2-P30 was transformed to a bacterium BL21-Codon Plus (DE3)-RIL strain and was expressed under IPTG induction. Cells were lysed by multiple rounds of sonication to obtain supernatant and inclusion body respectively. The inclusion body was washed with 2 mol/L and 4 mol/L urea to remove the nonspecific protein. The washed products dissolved in 8 mol/L urea were received by SDS-PAGE. Two rabbits were immunized with the fusion protein rROP2-P30 and sera from the rabbits were collected. Immune diffusion test, indirect ELISA and Western-blot were used to detect antibody titer and specificity of the immune serum against rROP2-P30. RESULTS: Immune diffusion test demonstrated that specific immune serum were obtained. Indirect ELISA confirmed that the antibody titer in the serum reached 1:12 800 and the rROP2-P30 was recognized by specific IgG in this serum by Western-blot analysis. CONCLUSION: Specific immune serum against the recombinant fusion protein rROP2-P30 has been prepared.


Assuntos
Soros Imunes/isolamento & purificação , Proteínas de Membrana/imunologia , Proteínas de Protozoários/imunologia , Proteínas Recombinantes de Fusão/imunologia , Toxoplasma/imunologia , Animais , Anticorpos Antiprotozoários/sangue , Anticorpos Antiprotozoários/imunologia , Western Blotting , Ensaio de Imunoadsorção Enzimática , Soros Imunes/imunologia , Plasmídeos/genética , Coelhos , Toxoplasma/genética
17.
World J Gastroenterol ; 11(9): 1297-302, 2005 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-15761967

RESUMO

AIM: To find a cost-effective method of preparation of short interfering RNAs based on cloning, fermentation, digestion and purification (CFDP) and test its feasibility to inhibit hepatitis B virus replication in cell culture. METHODS: We constructed an expression vector containing T7 and tac promoter in a head-to-head orientation. cDNA fragment of interest was cloned into this vector between the opposing promoters. dsRNAs were expressed with this vector in Escherichia coli, and purified by affinity chromatography using CF 11 column. They were digested by RNase III in a buffer containing manganese ions, then separated on 15% non-denaturing PAGE, and the siRNAs about 25 bp in length were recovered. siRNAs prepared with CFDP were co-transfected with target gene expression plasmid into human cell lines with lipofectamine 2,000 to test their inhibition efficiency. RESULTS: siRNAs corresponding to part of the hepatitis B virus polymerase gene (siHBVP) prepared by CFDP specifically and dramatically suppressed the virus protein expression. The HBsAg expression level was reduced to 10% that of the control by co-transfection of 60 nmol/L siHBVP in SMMC7721 cells. Dose-dependent effect on suppression of HBsAg and HBeAg expression was observed in HepG2 cells. The highest inhibition rate was kept at 70% during the six days after transfection of 7.5 nmol/L siHBVP. CONCLUSION: We show CFDP is a very promising method to prepare therapeutic agents in anti-virus applications.


Assuntos
Vírus da Hepatite B/crescimento & desenvolvimento , Vírus da Hepatite B/genética , RNA Interferente Pequeno/economia , RNA Interferente Pequeno/genética , Replicação Viral/genética , Sequência de Bases , Carcinoma Hepatocelular , Linhagem Celular Tumoral , Análise Custo-Benefício , Regulação Viral da Expressão Gênica , Proteínas de Fluorescência Verde/genética , Antígenos de Superfície da Hepatite B/genética , Antígenos E da Hepatite B/genética , Humanos , Neoplasias Hepáticas , Dados de Sequência Molecular , Plasmídeos/genética , RNA/genética , RNA de Cadeia Dupla/genética , Ribonuclease III , Transfecção
18.
Artigo em Chinês | MEDLINE | ID: mdl-16566209

RESUMO

OBJECTIVE: To clone and express the fused gene fragment coding rhoptry protein ROP2 and major surface protein P30 from Toxoplasma gondii as a preparation for the construction of the complex ROP2-P30 antigen by gene engineering. METHODS: The gene fragment encoding P30 was amplified by PCR from T. gondii RH strain and subcloned into the recombinant plasmid pUC119/ROP2 already constructed. The recombinant plasmid pUC119/ROP2-P30 was digested by Sac I/HindIII and inserted into the same site of expression vector pET28b. The recombinant plasmid of pET28b/ROP2-P30 was transformed to E. coli and expressed under the induction of IPTG. RESULTS: The gene fragment 700 bp encoding P30 was obtained from the total DNA of T. gondii by PCR. The recombinant plasmid pET28b/ROP2-P30 was successfully constructed, which was highly expressed in E. coli, a fusion protein with molecular weight of 69000. CONCLUSION: The fusion gene encoding the rhoptry protein ROP2 and the major surface protein P30 of T. gondii has been successfully cloned and expressed to be an expected recombinant fusion protein ROP2-P30 with molecular weight 69000.


Assuntos
Antígenos de Protozoários/biossíntese , Antígenos de Superfície/biossíntese , Proteínas de Membrana/biossíntese , Proteínas de Protozoários/biossíntese , Proteínas Recombinantes de Fusão/biossíntese , Toxoplasma/genética , Animais , Antígenos de Protozoários/genética , Antígenos de Superfície/genética , Clonagem Molecular , Escherichia coli/genética , Expressão Gênica , Proteínas de Membrana/genética , Plasmídeos/genética , Reação em Cadeia da Polimerase , Proteínas de Protozoários/genética
19.
Acta Paediatr Taiwan ; 45(1): 19-22, 2004.
Artigo em Inglês | MEDLINE | ID: mdl-15264701

RESUMO

Arterial switch operation (ASO) is considered the procedure of choice for transposition of great arteries (TGA). The results and long-term prognosis improved with recent advances in perioperative management. We herein analyze the clinical outcome of patients undergoing ASO at our institution during the past 3 years. From 2000 to 2002, 44 patients (30 male and 14 female) of TGA received ASO. Age at operation varied from 4 days to 6.6 years (median 14 days) with body weight ranged from 2.25 kg to 18.1 kg (median 3.3 kg). Palliative procedure prior to ASO was performed in 8 patients (18.8%). Normal coronary artery pattern was found in 28 patients (63.6%). The early mortality was 11% (5/44). Only associated ventricular septal defect (VSD) was a significant predictor for operative mortality (p=0.012). With a follow-up ranged from 11 to 44 months, the gradient of neo-pulmonary artery stenosis was 16.5 +/- 18.2 mmHg. Four patients (10%) received balloon dilatation and the other three (7.7%) underwent reoperation. The gradient of neo-aortic stenosis was 16.5 +/- 18.2 mmHg that needed to be dilated in three patients (7.7%). The probability free from reintervention was 73% at the 3rd postoperative year. One patient had moderate degree of pulmonary valve regurgitation and six had moderate neo-aortic valve regurgitation. In conclusion, the ASO can be performed in infants with satisfactory results, even in those with a body weight less than 2.5 kg. Only associated VSD was shown to be a risk factor.


Assuntos
Procedimentos Cirúrgicos Cardiovasculares/métodos , Comunicação Interventricular/cirurgia , Transposição dos Grandes Vasos/cirurgia , Cateterismo/métodos , Criança , Pré-Escolar , Feminino , Seguimentos , Comunicação Interventricular/mortalidade , Humanos , Lactente , Recém-Nascido , Masculino , Análise de Sobrevida , Taxa de Sobrevida , Taiwan , Transposição dos Grandes Vasos/mortalidade , Resultado do Tratamento
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