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Recent Pat Biotechnol ; 8(1): 102-7, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-22642822


Artemisinin, a potent antimalarial natural products isolated from aerial parts of Artemisia annua L. Many patents have been reported that the demand for artemisinin is exponentially increasing year after year due to increased incidences of drug resistant malaria throughout the world. Leaf explants were used frequently as target tissue to generate transgenic of Artemisia. annua L. However, obtaining a large number of transgenic lines through out the year is a laborious and delicate process. To circumvent this, we have developed a highly efficient leaf explant based Agrobacterium mediated transformation of A. annua L. plant. The gus gene was used as screenable marker to assess and optimize the performance of T-DNA delivery. The age of explant, kind of bacterial inoculation, suspension duration, infection times and co-culture conditions were optimized. The co-culture was carried out with Agrobacterium tumefaciens strain EHA105 under desiccation condition in the dark at 25-28 0C for 2-4 days. Complete analysis of transgene insertion demonstrated that the optimized method of transformation from leaf explants of A. annua L. was efficient and highly reproducible.

Agrobacterium/metabolismo , Artemisia annua/metabolismo , Agrobacterium/genética , Artemisia annua/citologia , Técnicas de Cocultura , Glucuronidase/genética , Glucuronidase/metabolismo , Folhas de Planta/citologia , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Brotos de Planta/citologia , Plasmídeos/genética , Plasmídeos/metabolismo , Transformação Genética
Recent Pat Biotechnol ; 5(3): 227-34, 2011 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22360470


In this report, rapid and effective shoot as well as root regeneration system through direct multiplication was successfully developed for Cichorium intybus L. Furthermore, the effect of exogenous growth regulators (TDZ and IAA) at different concentrations on the regulation process of the plant was also studied. Enhanced production of esculin in developed C. intybus L. was evaluated using leaf extract. Only on the expense of 20 days, regeneration was seen and very low dose of TDZ was seen to be more effective. When 0.02 mg/L of TDZ was combined with 1.5mg/L of IAA, nearly 100% of explants produced shoots with the highest number of regenerated shoots (85.37). With further increase in concentration (≥ 0.05 mg/L), the number of shoots per explants get decreased. A lower NAA to IBA ratio (1.0mg/L of IBA and 0.5mg/L of NAA) seemed to be more effective for root generation and considered to be the most effective combination among the tried groups. IBA was more effective in root development than NAA, but both were comparatively effective. On quantitative analysis by RP-HPLC, the 76.23% of Esculin were found in leaf extract of the in vitro developed C. intybus L. This amount was 26.77% higher than normal grown plants.

Chicória/crescimento & desenvolvimento , Cromatografia Líquida de Alta Pressão , Cromatografia de Fase Reversa , Esculina/análise , Chicória/efeitos dos fármacos , Chicória/metabolismo , Esculina/isolamento & purificação , Ácidos Indolacéticos/química , Ácidos Indolacéticos/farmacologia , Patentes como Assunto , Compostos de Fenilureia/química , Compostos de Fenilureia/farmacologia , Reguladores de Crescimento de Planta/química , Reguladores de Crescimento de Planta/farmacologia , Raízes de Plantas/efeitos dos fármacos , Raízes de Plantas/crescimento & desenvolvimento , Raízes de Plantas/metabolismo , Brotos de Planta/efeitos dos fármacos , Brotos de Planta/crescimento & desenvolvimento , Brotos de Planta/metabolismo , Tiadiazóis/química , Tiadiazóis/farmacologia
J Ayub Med Coll Abbottabad ; 22(3): 132-5, 2010.
Artigo em Inglês | MEDLINE | ID: mdl-22338438


BACKGROUND: Abdominal obesity is an increasing public health problem and is associated with a number of cardiovascular risk factors. The aim of this study was to determine the frequency of abdominal obesity and associated risk factors in patients of various ethnic groups presenting with acute coronary syndrome. METHODS: A total of 477 patients presenting with acute coronary syndrome to the National Institute of Cardiovascular Diseases, Karachi were studied. The sample was divided into 5 major ethnic groups, Muhajir, Punjabi, Sindhi, Pathan, Baluchi and Others. Waist circumference of each patient was taken at the level of iliac crest and abdominal obesity was defined according to the International Diabetes Federation criteria of 2005 for South Asians as waist circumference > or = 90 Cm in males and > or = 80 Cm in females. Patients were also assessed for diabetes mellitus, hypertension, smoking, low HDL and elevated triglycerides. RESULTS: Out of 477 patients (355 males, 122 females), abdominal obesity was present in 318 (67%) patients, 62% of males and 81% of females were obese. Abdominal obesity was 62% in Muhajir, 78% in Punjabi, 67% in Sindhi, 68% in Pathan, 59% in Baluchi and 64% in others. Hypertension was present in 46%, Diabetes mellitus in 32%, Smoking in 33%, Low level of High density lipoprotein in 85.5% and raised triglycerides in 35% of patients. CONCLUSION: Abdominal obesity in Punjabi ethnic group is more common as compared to other ethnic groups. Low level of high density lipoprotein is almost universally present in patients with acute coronary syndrome.

Síndrome Coronariana Aguda/etnologia , Obesidade Abdominal/etnologia , Síndrome Coronariana Aguda/epidemiologia , Diabetes Mellitus/epidemiologia , Diabetes Mellitus/etnologia , Feminino , Humanos , Hipertensão/epidemiologia , Hipertensão/etnologia , Lipídeos/sangue , Masculino , Pessoa de Meia-Idade , Obesidade Abdominal/epidemiologia , Paquistão/epidemiologia , Fatores de Risco , Fumar/epidemiologia , Fumar/etnologia , Circunferência da Cintura
J Phys Chem A ; 113(41): 10886-90, 2009 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-19722598


We combine classical molecular dynamics simulations and quantum density functional theory calculations to study the temperature effects on the electron affinity of the water octamer. The atomistic simulations provide a sample of the cluster's conformations as a function of the temperature, on which the density functional calculations are carried on. As the temperature increases, the cluster undergoes its characteristic phase change from a cubic, solidlike structure to a liquidlike state. This phase change is also reflected by an increase on the total dipole moment of the cluster. The quantum calculations indicate that the large dipole moment conformations have a positive electron affinity. Relaxing the high temperature conformations of the cluster anion to its local minimum, the average vertical detachment energy is calculated and shows a clear tendency to increase as the temperature increases. The analysis of the high temperatures conformations reveals that origin of higher values of the vertical detachment energy is not the stability of the negative octamer but the high energy of the corresponding neutral cluster.