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1.
Braz. j. oral sci ; 20: e210525, jan.-dez. 2021. ilus
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-1253928

RESUMO

Aim: Recent reports indicate that deproteinization of acid-etched dentin surface can extend penetration depth of adhesive agents. The main goal of the present research was to investigate the deproteinization effect of Nd:YAG and diode 940 lasers on acid-etched dentin and microleakage grade in class V composite restorations. Methods: 36 extracted human premolar teeth were selected to make standard buccal and lingual class V cavities. These samples were randomly split into three sub-groups: 1.Control group, in which composite was applied for restoration after etch and bonding process without deproteinization; 2.Nd:YAG laser group, in which the teeth were deproteinized with Nd:YAG laser after etching and painting internal surfaces of cavities with Van Geison stain and then composite restorations applied just as control group; 3.Diode laser group, in which the process was similar to Nd:YAG laser group, but instead, diode 940 laser was irradiated. The teeth were bisected into two equal longitudinal buccal and lingual halves. Marginal microleakage of samples was scored by using a stereomicroscope. Kruskal- Wallis, Mann-Whitney U and Fisher's statistical tests were employed for analysis of the obtained data. Results: A significant reduction in marginal microleakage was observed for both groups treated with laser (Nd:YAG and diode 940)compared to control (p=0.001 & p=0.047). There was no significant difference in marginal microleakage between Nd:YAG laser and diode 940groups (P = 0.333). Conclusion: Nd:YAG and diode 940 laser deproteinization of acid-etched dentin decreased the marginal microleakage of in-vitro class V resin composite restorations


Assuntos
Humanos , Condicionamento Ácido do Dente , Adesivos Dentinários , Infiltração Dentária , Lasers
2.
Artigo em Inglês | MEDLINE | ID: mdl-34480699

RESUMO

Inspired by a circular economy paradigm, an evolving momentum of policies and legislations aims to close the loop of product lifecycles through improved level of recycling, remanufacturing, and reuse, with the objective of adding value to the economy while not endangering the environment. However, the trade-off between the environmental and economic sustainability of designing business processes is inevitable. To address this trade-off in the supply chain context, competing objectives regarding both cost minimization and reduction in carbon emission should be simultaneously considered and integrated into a comprehensive model. This complexity is however elevated when uncertainty of demand is taken into consideration. In this study, the design of a closed-loop supply chain is investigated where competing objectives of cost and sustainability of supply chain operations are evaluated under demand uncertainty. Augmented Weighted Tchebycheff (AWT) and ε-constraint methods are employed to address the multi-objectivity of the problem while a robust optimization approach is applied to deal with the demand uncertainty. The results confirm that the proposed approach provides efficient solutions for designing a green closed-loop supply chain network.

3.
J Drug Target ; : 1-10, 2021 Jul 12.
Artigo em Inglês | MEDLINE | ID: mdl-34116599

RESUMO

The purpose of this research was to enhance the transdermal delivery of diclofenac sodium niosomal formulations. To characterise the obtained niosomes, SEM, XRPD, DSC and ATR-FTIR were employed. The size of the niosomes increased from 158.00 ± 6.17 to 400.87 ± 4.99 nm when cholesterol was incorporated into the formulations. It was observed that the zeta potential of niofenac varies from -25.40 ± 1.352 to -43.13 ± 1.171 mV when the cholesterol percentage decreased from 2% to 0.2%. The higher entrapment efficiency percentage (63.70 ± 0.18%) was obtained for the formulations with larger particle sizes and higher cholesterol content. The optimised niofenac formulation showed a controlled release fashion where 61.71 ± 0.59% of the drug released within 24 h. The results showed that the value of permeated diclofenac sodium through the skin layers was higher for the niofenac gel formulation (242.3 ± 31.11 µg/cm2) compared to simple gel formulation (127.40 ± 27.80 µg/cm2). Besides, niofenac formulation outperformed the anti-inflammatory activities in the formalin test compared to the control and diclofenac simple gel group. The licking time was significantly lower in both early (40.2 ± 7.3 s) and late stages (432.4 ± 31.7 s) for niofenac compared to conventional formulation (early stage 130.4 ± 8.73 s and late stage 660.6 ± 123.73 s). This study indicates that niosomal formulations can improve drug therapeutic effects by increasing drug delivery to specific sites.

4.
Genomics ; 113(4): 2793-2799, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34118384

RESUMO

OTOG encodes for otogelin, a component of the tectorial membrane. This gene is associated with nonprogressive mild-to-moderate hearing loss. However, no studies have yet identified the association between OTOG variation and severe-to-profound hearing loss. Therefore, to address this issue, a family-based whole-exome sequencing strategy (WES) was carried out. Two unrelated Iranian families with non-syndromic hearing loss were identified, and WES was conducted on one selected candidate from each family. As a result, a rare homozygous missense variant, OTOG (c.C2383T:p.R795C), was detected in both of the subjected probands, and segregation analysis confirmed the c.C2383T variant in seven cases of severe-to-profound hearing loss. Additionally, the results from the protein modeling demonstrated that the altered position of a few disulfide bonds in the TIL domain may have a deleterious impact on protein stability and normal functionality. In conclusion, it seems that the homozygosity of the OTOG c.C2383T mutation sheds light on hearing loss pathobiology. Nevertheless, further studies are required to unravel the precise function of OTOG mutation, which is potentially associated with severe-to-profound hearing loss.

5.
Cell Oncol (Dordr) ; 44(3): 495-523, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33759063

RESUMO

BACKGROUND: Chimeric antigen receptor (CAR)-modified T cell therapy has shown great potential in the immunotherapy of patients with hematologic malignancies. In spite of this striking achievement, there are still major challenges to overcome in CAR T cell therapy of solid tumors, including treatment-related toxicity and specificity. Also, other obstacles may be encountered in tackling solid tumors, such as their immunosuppressive microenvironment, the heterogeneous expression of cell surface markers, and the cumbersome arrival of T cells at the tumor site. Although several strategies have been developed to overcome these challenges, aditional research aimed at enhancing its efficacy with minimum side effects, the design of precise yet simplified work flows and the possibility to scale-up production with reduced costs and related risks is still warranted. CONCLUSIONS: Here, we review main strategies to establish a balance between the toxicity and activity of CAR T cells in order to enhance their specificity and surpass immunosuppression. In recent years, many clinical studies have been conducted that eventually led to approved products. To date, the FDA has approved two anti-CD19 CAR T cell products for non-Hodgkin lymphoma therapy, i.e., axicbtagene ciloleucel and tisagenlecleucel. With all the advances that have been made in the field of CAR T cell therapy for hematologic malignancies therapy, ongoing studies are focused on optimizing its efficacy and specificity, as well as reducing the side effects. Also, the efforts are poised to broaden CAR T cell therapeutics for other cancers, especially solid tumors.

6.
Spectrochim Acta A Mol Biomol Spectrosc ; 255: 119697, 2021 Jul 05.
Artigo em Inglês | MEDLINE | ID: mdl-33774416

RESUMO

In the current research, an analytical method was proposed for the quantitative determination of surface tension of anionic surfactant solutions in the presence of hydrophilic silica nanoparticles using attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy and chemometric methods. The surface tension behavior of anionic surfactant solutions considerably changes by the addition of silica nanoparticles with different particle size. The spectral data of solutions were used for prediction of surface tension using two calibration methods based on support vector machine regression (SVM-R) as a non-linear algorithm and partial least squares regression (PLS-R) as a linear algorithm. For preprocessing of data, baseline correction and standard normal variate (SNV) were also applied. Root mean square error of prediction (RMSEP) in SVM-R and PLS-R methods were 4.203 and 4.507, respectively. Considering the complexity of the samples, the SVM-R model was found to be reliable. The proposed method is fast and easy for measurement of the surface tension of surfactant solutions without any sample preparation step in chemical enhanced oil recovery (C-EOR).

7.
Biochem Genet ; 59(1): 31-41, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32720141

RESUMO

Deregulation of the renin-angiotensin system (RAS) plays an important role in suicide. Angiotensin converting enzyme (ACE) gene is a key component in this system. The relationship between insertion/deletion (I/D) polymorphism of ACE gene with suicide attempt (SA) is controversial. According to previous studies, allele D in this polymorphism has been considered as a potential risk factor for suicide. However, no study has been conducted in Iran to investigate this matter. This case-control study has focused on investigating the association of ACE I/D polymorphism (rs1799752) with SA in an Iranian population. The frequency of genotypes was 14% for II, 55% for ID, and 31% for DD in the case group (100 persons), and 18% for II, 74% for ID, and 8% for DD in control group (100 persons). Results show there was a significant difference in the distribution of ACE I/D polymorphism genotypes in men with SA compared to controls, as well as in women with SA compared to controls. Also, there was a significant association between DD genotype and the risk of SA compared to II genotype as reference. The severity of depression was significantly different between DD and II genotypes in SA group. According to the results, we suggest that the presence of DD genotype is possibly associated with an increased risk of SA. Maybe part of that is related to severity of depression in DD genotypes carriers of ACE I/D polymorphism.

8.
J Dent (Shiraz) ; 21(3): 158-176, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-33062809

RESUMO

Statement of the Problem: Dental caries is a global health issue, which imposes a great deal on individuals and the community. Purpose: The main purpose of this study was to identify the dental caries status and/or DMFT/dmft data and its related factors in Iran. Materials and Method: The search performed in the online databases to identify all literature published up to Oct 2018. The random effects model was applied to pool analysis and verses. Funnel plots and Egger test used to examine publication bias. All analyses were carried out with R software version 3.2.1 and STATA (version 11.1). Results: 69 studies selected as eligible for final analysis in which all subjects were in age range less than 18 years old except 4; so, all results and analyzes were only calculated in this age group. The total rate of dental caries was 72.8% (95%CI, 69.2-76.4%) and the mean of dental caries was 2.33 (95% CI, 2.12-2.54) based on DMFT values and 3.86 (95% CI, 3.49-4.22) based on the dmft values. These rates were almost similar in both sexes. In addition, these trend were higher in rural than urban regions. Subgroup analysis found a direct relationship between DMFT index and age, while the reverse was true for relationship between dmft and age. The mean dental caries were higher in children with low socioeconomic status, low family income, low educated and unemployed parents, crowded families, excessive carbohydrate intake, and less toothbrushes frequency. Conclusion: The present study showed high prevalence and experience of dental caries among children and adolescents (<18 years of age) in Iran. This illustrates ineffective oral health national preventive programs and lack of educational measures. New preventive procedures, practical educational programs, and modern therapeutic methods are needed to improve oral health status.

9.
Eur J Pharmacol ; 886: 173509, 2020 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-32889065

RESUMO

In spite of advancements in breast cancer therapy, this disease is still one of the significant causes of women fatalities globally. Dysregulation of miRNA plays a pivotal role in the initiation and progression of cancer. Therefore, the administration of herbal compounds with anticancer effects through controlling microRNA expression can be considered as a promising therapy for cancer. Oleuropein is the most prevalent phenolic compound in olive. Given its domestic consumption, low cost, and nontoxicity for human beings, oleuropein can be used in combination with the standard chemotherapy drugs. To this end, we examined the effect of oleuropein on two breast cancer cell lines (MCF7 and MDA-MB-231). Our findings revealed that oleuropein significantly decreased cell viability in a dose- and time-dependent manner, while it increased the apoptosis in MCF7 and MDA-MB-231 cells. In the presence of oleuropein, the expression levels of miR-125b, miR-16, miR-34a, p53, p21, and TNFRS10B increased, while that of bcl-2, mcl1, miR-221, miR-29a and miR-21 decreased. The findings pointed out that oeluropein may induce apoptosis via not only increasing the expression of pro-apoptotic genes and tumor suppressor miRNAs, but also decreasing the expression of anti-apoptotic genes and oncomiR. Consequently, oleuropein can be regarded as a suitable herbal medication for cancer therapy.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Proteínas Reguladoras de Apoptose/efeitos dos fármacos , Apoptose/efeitos dos fármacos , Neoplasias da Mama/tratamento farmacológico , Glucosídeos Iridoides/farmacologia , Transdução de Sinais/efeitos dos fármacos , Antineoplásicos Fitogênicos/uso terapêutico , Neoplasias da Mama/patologia , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Biologia Computacional , Relação Dose-Resposta a Droga , Feminino , Regulação Neoplásica da Expressão Gênica , Genes Supressores de Tumor/efeitos dos fármacos , Humanos , Glucosídeos Iridoides/uso terapêutico , MicroRNAs/biossíntese , MicroRNAs/genética
10.
Complement Ther Med ; 52: 102452, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32951715

RESUMO

OBJECTIVES: Many factors implicated in the pathogenesis of non-alcoholic fatty liver disease (NAFLD) are including oxidative stress, insulin resistance and abnormal production of adipokines. The aim of this clinical trial was to evaluate the effect of melatonin supplement on some important biochemical markers and signs related to NAFLD. DESIGN: A randomized double-blind, placebo-controlled, clinical trial. SETTING: Twenty-four participants in the melatonin group and 21 participants in the placebo group completed the study. INTERVENTION: Participants received 6 mg melatonin or placebo daily, 1 h before bedtime. The intervention period was 12 weeks. MAIN OUTCOME MEASURES: Anthropometric measurements, systolic and diastolic blood pressure, liver enzymes, high sensitive C­reactive protein (hs-CRP), fatty liver grade, also leptin and adiponectin serum levels, were measured at the baseline and the end of intervention. RESULTS: A significant improvement was observed in weight (p = 0.043), waist circumference (p = 0.027), abdominal circumference (p = 0.043), systolic (p = 0.039), and diastolic (p = 0.015) blood pressure, leptin serum levels (p = 0.032), hs-CRP (p = 0.024), alanine aminotransferase (p = 0.011), aspartate aminotransferase (p = 0.034), also the grade of fatty liver (p = 0.020) in melatonin treated group compared with the placebo. CONCLUSIONS: Administration of 6 mg/day melatonin had improvement effect on many factors related to NAFLD such as liver enzymes, hs-CRP, anthropometric measurements, blood pressure, leptin serum levels and the grade of fatty liver.


Assuntos
Melatonina/administração & dosagem , Hepatopatia Gordurosa não Alcoólica/tratamento farmacológico , Adulto , Antioxidantes/administração & dosagem , Biomarcadores/sangue , Pressão Sanguínea/efeitos dos fármacos , Pesos e Medidas Corporais , Método Duplo-Cego , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
11.
Int J Mol Cell Med ; 9(1): 1-32, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32832482

RESUMO

Neurodegeneration is a gradual mechanism of neuronal loss arising from numerous cellular and molecular events such as mitochondrial dysfunction, oxidative stress, inflammation, and apoptosis, and the consequence of these processes is neuroplasticity impairment, cognitive diseases, mood-related diseases, and normal cellular activity. Over the last year, major advances have been made in the field of the introduction of herbal compounds with neuroprotective efficacy, one of which is curcumin. Curcumin (diferuloylmethane) is the most abundant turmeric component extracted from the Curcuma longa plant rhizomes. Accumulating evidence indicates that curcumin may induce mitochondrial biogenesis and can function as an antioxidant, anti-inflammatory, and anti-apoptotic agent, which may be used effectively to treat chronic neurodegenerative diseases and any situation in which the neurodegeneration process takes place. Curcumin has been shown to play a critical role in activating two essential signaling pathways phosphatidylinositol-3(PI3)/ protein kinase B(Akt)/ glycogen synthase kinase-3 (GSK3) and PI3/Akt/cAMP response element-binding protein (CREB)/brain-derived neurotrophic factor (BDNF) and preventing the incidence of neurodegeneration via these two pathways. Curcumin's protective functions against neural cell degeneration due to mitochondrial dysfunction and consequent events such as oxidative stress, inflammation, and apoptosis in neural cells have been documented and clinical data have increased to suggest that curcumin may be a standard candidate as a neuroprotective agent. Therefore, in this review, we summarized the clinical and experimental studies and interpreted the key contributory mechanisms of neuroprotective properties of curcumin in neurodegenerative diseases and disorders. We also tried to understand the function of PI3/Akt/GSK3 and PI3/Akt/CREB/BDNF signaling pathways in the neuroprotective properties of curcumin and tried to evaluate their association with antioxidant, anti-inflammatory, anti-apoptosis and biogenesis effects of mitochondria.

12.
Artigo em Inglês | MEDLINE | ID: mdl-32850705

RESUMO

The rate-limiting component of cellulase for efficient degradation of lignocellulosic biomass through the enzymatic route depends on glucosidase's sensitivity to the end product (glucose). Therefore, there is still a keen interest in finding glucose-tolerant ß-glucosidase (BGL) that is active at high glucose concentrations. The main objective of this study was to identify, isolate, and characterize novel highly glucose-tolerant and halotolerant ß-glucosidase gene (PersiBGL1) from the mixed genome DNA of sheep rumen metagenome as a suitable environment for efficient cellulase by computationally guided experiments instead of costly functional screening. At first, an in silico screening approach was utilized to find primary candidate enzymes with superior properties. The structure-dependent mechanism of glucose tolerance was investigated for candidate enzymes. Among the computationally selected candidates, PersiBGL1 was cloned, isolated, and structurally characterized, which achieved very high activity in relatively high temperatures and alkaline pH and was successfully used for the hydrolysis of cellobiose. This enzyme exhibits a very high glucose tolerance, with the highest inhibition constant K i (8.8 M) among BGLs reported so far and retained 75% of its initial activity in the presence of 10 M glucose. Furthermore, a group of multivalent metal, including Mg2+, Mn2+, and Ca2+, as a cofactor, could improve the catalytic efficiency of PersiBGL1. Our results demonstrated the power of computational selected candidates to discover novel glucose tolerance BGL, effective for the bioconversion of lignocellulosic biomass.

13.
Anesth Pain Med ; 10(2): e103532, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32754435

RESUMO

Background: Several studies have demonstrated an association between chronic pain and impairment of cognitive capabilities. Since the number sense is one of the cognitive ability involved in the evaluation of pain intensity using some pain measurement tools, impairment of number sense may impact pain assessment. Therefore, the validity of number-based pain assessment tools should be re-evaluated. Objectives: This study aimed to determine whether number sense is altered in chronic and acute pain patients compared to healthy subjects. Methods: Overall, 150 participants were recruited and divided into the three groups of controls, chronic and acute pain patients. Pain intensity was evaluated using numeric and verbal rating scales as pain assessment tools. Number sense was assessed using number naming, number marking, and line bisection tasks. Deviation from correct answers was measured for each task. Results: Patients with chronic pain (migraine headache) had higher pain intensity scores than acute pain subjects. Chronic pain patients showed significant deviation from the expected responses compared to controls in the line bisection task. Conclusions: Chronic pain patients may have impaired number sense and may differently use number-based pain assessment tools in comparison with healthy individuals.

14.
Front Oncol ; 10: 531, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32695658

RESUMO

Notch suppression by gamma-secretase inhibitors is a valid approach against melanoma. However, most of studies have evaluated the short-term effect of DAPT on tumor cells or even cancer stem cells. In the present study, we surveyed the short-term and long-term effects of DAPT on the stem cell properties of A375 and NA8 as melanoma cell lines. The effects of DAPT were tested both in vitro and in vivo using xenograft models. In A375 with B-raf mutation, DAPT decreased the level of NOTCH1, NOTH2, and HES1 as downstream genes of the Notch pathway. This was accompanied by enhanced apoptosis after 24 h treatment, arrest in the G2-M phase, and impaired ability of colony and melanosphere formation at the short term. Moreover, tumor growth also reduced during 13 days of treatment. However, long-term treatment of DAPT promoted tumor growth in the xenograft model and enhanced the number and size of colonies and spheroids in vitro. The gene expression studies confirmed the up-regulation of Wnt and Notch downstream genes as well as AXIN1, CSNK2A3, and CEBPA2 following the removal of Notch inhibitor in vitro and in the xenograft model. Moreover, the Gompertz-based mathematical model determined a new drug resistance term in the present study. Our data supported that the long-term and not short-term inhibition of Notch by DAPT may enhance tumor growth and motility through up-regulation of AXIN1, CSNK2A3, and CEBPA2 genes in B-raf mutated A375 cells.

15.
Physiol Mol Biol Plants ; 26(3): 459-474, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32205923

RESUMO

To study the possibility of increasing the drought tolerance of common bean with the exogenous application of 24-epibrassinolide (EBL), an experiment was conducted in 2016 and 2017. In this experiment, two irrigation levels (optimal irrigation and drought stress) were applied to the main plots and two common bean genotypes (Kusha cultivar and COS16 genotype) and four EBL concentrations (0, 2, 4, and 6 µM) were allocated to sub-plots as factorial. In the flowering stage, drought stress was applied and plants were sprayed with EBL. The results showed that drought stress reduced relative water content (RWC) and increased proline content, malondialdehyde (MDA) content, and antioxidant enzymes activity. However, exogenous application of EBL reduced the seed yield loss and increased the drought stress tolerance in both common bean genotypes by decreasing the MDA content and increasing the RWC, proline content, antioxidant enzymes activity, and nitrate reductase activity. It can be concluded that foliar spray of 4 µM EBL as the best concentration may increase the seed yield and enhance the drought stress tolerance of common bean. Also, Cu/Zn-SOD was up-regulated in response to the drought stress and exogenous EBL. The COS16 genotype showed better response to the drought stress and exogenous EBL than the Kusha cultivar, because of the higher up-regulation of Cu/Zn-SOD in this genotype compared to the Kusha cultivar. Therefore, EBL can be used as a plant growth regulator to enhance drought stress tolerance and minimize the seed yield loss of common bean caused by water deficit.

16.
Spectrochim Acta A Mol Biomol Spectrosc ; 232: 118157, 2020 May 05.
Artigo em Inglês | MEDLINE | ID: mdl-32106028

RESUMO

Classification based on °API gravity is very important to estimate the parameters related to the extraction, purification, toxicity, and pricing of crude oils. Spectroscopy methods show some advantages over ASTM and API methods for crude oil analysis. The attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy coupled with chemometric methods has been applied as a quick and non-destructive method for crude oil analysis. In this work, a new analytical method using ATR-FTIR spectroscopy associated with chemometric methods were proposed for adressing regression and classification tasks for crude oils analysis based on °API gravity values. The designed methods are rapid, economic, and nondestructive ways in production process of oil industry. The spectral data were used for estimation of °API gravity using two approaches according to PLS-R and SVM-R algorithm, separately. The ATR-FTIR spectral data were also analyzed by classification method using the partial least squares-discriminant analysis (PLS-DA) for crude oil classification. The samples were classified into three classes based on their °API gravity values. The SVM-R model showed better results than PLS-R for °API gravity values using the F-test at 95% of confidence. The result of classification, showed about 100% accuracy and a zero classification error for calibration and prediction samples in PLS-DA algorithm.

17.
Biomed Pharmacother ; 121: 109635, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31739165

RESUMO

Breast cancer is the most common type of cancer among women. Therefore, discovery of new and effective drugs with fewer side effects is necessary to treat it. Sulforaphane (SFN) is an organosulfur compound obtained from cruciferous plants, such as broccoli and mustard, and it has the potential to treat breast cancer. Hence, it is vital to find out how SFN targets certain genes and cellular pathways in treating breast cancer. In this review, molecular targets and cellular pathways of SFN are described. Studies have shown SFN inhibits cell proliferation, causes apoptosis, stops cell cycle and has anti-oxidant activities. Increasing reactive oxygen species (ROS) produces oxidative stress, activates inflammatory transcription factors, and these result in inflammation leading to cancer. Increasing anti-oxidant potential of cells and discovering new targets to reduce ROS creation reduces oxidative stress and it eventually reduces cancer risks. In short, SFN effectively affects histone deacetylases involved in chromatin remodeling, gene expression, and Nrf2 anti-oxidant signaling. This review points to the potential of SFN to treat breast cancer as well as the importance of other new cruciferous compounds, derived from and isolated from mustard, to target Keap1 and Akt, two key regulators of cellular homeostasis.


Assuntos
Brassicaceae/química , Neoplasias da Mama/tratamento farmacológico , Isotiocianatos/farmacologia , Isotiocianatos/uso terapêutico , Animais , Feminino , Humanos , Preparações de Plantas/farmacologia , Preparações de Plantas/uso terapêutico
18.
Front Immunol ; 11: 585819, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33519807

RESUMO

Regulatory T cells (Tregs) are an immunosuppressive subgroup of CD4+ T cells which are identified by the expression of forkhead box protein P3 (Foxp3). The modulation capacity of these immune cells holds an important role in both transplantation and the development of autoimmune diseases. These cells are the main mediators of self-tolerance and are essential for avoiding excessive immune reactions. Tregs play a key role in the induction of peripheral tolerance that can prevent autoimmunity, by protecting self-reactive lymphocytes from the immune reaction. In contrast to autoimmune responses, tumor cells exploit Tregs in order to prevent immune cell recognition and anti-tumor immune response during the carcinogenesis process. Recently, numerous studies have focused on unraveling the biological functions and principles of Tregs and their primary suppressive mechanisms. Due to the promising and outstanding results, Tregs have been widely investigated as an alternative tool in preventing graft rejection and treating autoimmune diseases. On the other hand, targeting Tregs for the purpose of improving cancer immunotherapy is being intensively evaluated as a desirable and effective method. The purpose of this review is to point out the characteristic function and therapeutic potential of Tregs in regulatory immune mechanisms in transplantation tolerance, autoimmune diseases, cancer therapy, and also to discuss that how the manipulation of these mechanisms may increase the therapeutic options.


Assuntos
Imunoterapia/métodos , Linfócitos T Reguladores/imunologia , Animais , Humanos
19.
Comput Methods Programs Biomed ; 175: 25-33, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31104712

RESUMO

OBJECTIVES: There is an association between long and thick myocardial bridging (MB), haemodynamic perturbations and increased risk of myocardial infarction. This study aims to investigate the alteration in coronary haemodynamics with increasing the length of MB. METHODS: Angiography and intravascular ultrasound were performed in 10 patients with varying length of MB in the left anterior descending (LAD) artery. In silico models of MB were developed based on the reconstructed three-dimensional model of the LAD. The entire LAD was divided into 3 segments, proximal (pre-bridge), bridge and distal (post-bridge). Transient computational fluid dynamics simulations were performed to derive distribution of blood residence time and wall shear stress (WSS) over entire vessel including proximal, bridge and distal segments. RESULTS: With increasing the length of MB, a decreasing trend was observed in the WSS over proximal segment whereas an increasing trend was found in the WSS over bridge segment. When patients were divided into 2 groups based on the average length of MB in the whole cohort (Lave = 23.92 mm), patients with bridges longer than Lave had smaller WSS and higher residence time in the proximal segment compared to those with bridges shorter than Lave (0.59 ± 0.31 vs 0.21 ± 0.14 Pa and 0.0021 ± 0.0015 vs 0.0045 ± 0.0021 s). In contrast, patients with bridges longer than Lave had greater WSS in the bridge segment compared to those with bridges shorter than Lave (1.37 ± 1.66 vs 2.53 ± 3.14 Pa). No significant difference was found in the distal WSS of patients with short and long bridges. CONCLUSION: Our findings revealed a direct relationship between the length of MB and haemodynamic perturbations in the proximal segment such that the increased length of MB is associated with decreased WSS and increased residence time.


Assuntos
Vasos Coronários/diagnóstico por imagem , Cardiopatias Congênitas/fisiopatologia , Hemodinâmica , Ponte Miocárdica/fisiopatologia , Adulto , Idoso , Algoritmos , Artérias , Simulação por Computador , Angiografia Coronária , Vasos Coronários/anatomia & histologia , Vasos Coronários/fisiopatologia , Feminino , Cardiopatias Congênitas/complicações , Humanos , Hidrodinâmica , Processamento de Imagem Assistida por Computador , Imageamento Tridimensional , Masculino , Pessoa de Meia-Idade , Modelos Cardiovasculares , Movimento (Física) , Ponte Miocárdica/complicações , Resistência ao Cisalhamento , Estresse Mecânico , Ultrassonografia
20.
Spectrochim Acta A Mol Biomol Spectrosc ; 220: 117049, 2019 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-31141782

RESUMO

An analytical method was proposed for quantitative determination of rheological properties of polyacrylamide (PAM) solution in the presence of SiO2 nanoparticle and NaCl. The viscosity of PAM-SiO2 nanohybrid solution was predicted using attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy in the wavenumber range of 800-3000 cm-1 and chemometrics methods. Support vector machine regression (SVM-R) as a non-linear multivariate calibration procedure and partial least squares regression (PLS-R) as a linear procedure were applied for calibration. Preprocessing methods such as baseline correction and standard normal variate (SNV) were also utilized. Root mean square error of prediction (RMSEP) in SNV-SVM and SNV-PLS methods were 3.231 and 6.302, respectively. Considering the complexity of the samples, the SVM-R model was found to be reliable. The proposed method is rapid and simple without any sample preparation step for measurement of the viscosity of polymer solutions in chemical enhanced oil recovery (CEOR).

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