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1.
Sci Rep ; 9(1): 13967, 2019 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-31562359

RESUMO

The interaction of ionizing radiation with matter is of critical importance in numerous areas of science and technology like space and vacuum technology and even medicine and biotechnology. Secondary electron emission is a consequence of electron irradiation on materials. We achieve extremely low secondary electron emission yield values smaller than 0.2, even up to incident electron energies ~1 keV, due to an undocumented synergy between neighbouring metal and dielectric domains in composite samples. To investigate this experimental discovery, we propose a simple 3D model where the dielectric and metallic domains are arranged in parallel and interleaved. The proposed surface profile has a triangular shape to model the surface roughness. We obtain a continuous equation to describe the electric field that arises between grounded conductors and charged dielectrics domains. The calculated trajectories of secondary electrons in this 3D geometry are used to predict dynamic secondary emission yield, which strongly depends on the charge accumulated in the dielectric domains. This research paves the way to design new materials of low secondary emission yield, addressing the technological problem not yet resolved to inhibit the electron avalanche in RF equipment that limit their maximum working power.

2.
Biotechnol Bioeng ; 2019 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-31429928

RESUMO

Escherichia coli is a heavily used platform for the production of biotherapeutic and other high-value proteins, and a favored strategy is to export the protein of interest to the periplasm to simplify downstream processing and facilitate disulfide bond formation. The Sec pathway is the standard means of transporting the target protein but it is unable to transport complex or rapidly folding proteins because the Sec system can only transport proteins in an unfolded state. The Tat system also operates to transport proteins to the periplasm, and it has significant potential as an alternative means of recombinant protein production because it transports fully folded proteins. Here, we have tested the Tat system's full potential for the production of biotherapeutics for the first time using fed-batch fermentation. We expressed human growth hormone (hGH) with a Tat signal peptide in E. coli W3110 "TatExpress" strains that contain elevated levels of the Tat apparatus. This construct contained four amino acids from TorA at the hGH N-terminus as well as the initiation methionine from hGH, which is removed in vivo. We show that the protein is efficiently exported to the periplasm during extended fed-batch fermentation, to the extent that it is by far the most abundant protein in the periplasm. The protein was shown to be homogeneous, disulfide bonded, and active. The bioassay showed that the yields of purified periplasmic hGH are 5.4 g/L culture whereas an enzyme-linked immunosorbent assay gave a figure of 2.39 g/L. Separate analysis of a TorA signal peptide linked to hGH construct lacking any additional amino acids likewise showed efficient export to the periplasm, although yields were approximately two-fold lower.

3.
Microb Cell Fact ; 18(1): 19, 2019 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-30696436

RESUMO

BACKGROUND: The Twin-arginine translocation (Tat) pathway of Escherichia coli has great potential for the export of biopharmaceuticals to the periplasm due to its ability to transport folded proteins, and its proofreading mechanism that allows correctly folded proteins to translocate. Coupling the Tat-dependent protein secretion with the formation of disulfide bonds in the cytoplasm of E. coli CyDisCo provides a powerful platform for the production of industrially challenging proteins. In this study, we investigated the effects on the E. coli cells of exporting a folded substrate (scFv) to the periplasm using a Tat signal peptide, and the effects of expressing an export-incompetent misfolded variant. RESULTS: Cell growth is decreased when either the correctly folded or misfolded scFv is expressed with a Tat signal peptide. However, only the production of misfolded scFv leads to cell aggregation and formation of inclusion bodies. The comprehensive proteomic analysis revealed that both conditions, recombinant protein overexpression and misfolded protein accumulation, lead to downregulation of membrane transporters responsible for protein folding and insertion into the membrane while upregulating the production of chaperones and proteases involved in removing aggregates. These conditions also differentially affect the production of transcription factors and proteins involved in DNA replication. The most distinct stress response observed was the cell aggregation caused by elevated levels of antigen 43. Finally, Tat-dependent secretion causes an increase in tatA expression only after induction of protein expression, while the subsequent post-induction analysis revealed lower tatA and tatB expression levels, which correlate with lowered TatA and TatB protein abundance. CONCLUSIONS: The study identified characteristic changes occurring as a result of the production of both a folded and a misfolded protein, but also highlights an exclusive unfolded stress response. Countering and compensating for these changes may result in higher yields of pharmaceutically relevant proteins exported to the periplasm.


Assuntos
Escherichia coli/genética , Estresse Oxidativo , Dobramento de Proteína , Proteoma , Escherichia coli/metabolismo , Proteínas de Escherichia coli/genética , Proteínas de Escherichia coli/metabolismo , Proteínas de Membrana Transportadoras , Sinais Direcionadores de Proteínas , Transporte Proteico , Proteômica , Proteínas Recombinantes de Fusão/química , Anticorpos de Cadeia Única/química
4.
Microbiol Res ; 218: 97-107, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30454663

RESUMO

In Escherichia coli, the Twin-arginine translocation (Tat) pathway secretes a set of folded proteins with important physiological functions to the periplasm and outer membrane. The loss of Tat secretion impairs outer membrane integrity and leads to decreased cell growth. Only recently, the Tat pathway has gained more attention due to its essential role in bacterial virulence and applications in the production of fully folded heterologous proteins. In this study, we investigated the influence of the deletion of all active Tat pathway components on the E. coli cells. The comprehensive proteomic analysis revealed activation of several stress responses and experimentally confirmed the dependence of certain proteins on the Tat system for export. We observed that a tat deletion triggers protein aggregation, membrane vesiculation, synthesis of colanic acid and biofilm formation. Furthermore, the mislocalization of Tat-dependent proteins disturbs iron and molybdenum homeostasis and impairs the cell envelope integrity. The results show that the functional Tat pathway is important for the physiological stability and that its dysfunction leads to a series of severe changes in E. coli cells.


Assuntos
Escherichia coli/genética , Escherichia coli/metabolismo , Transporte Proteico/genética , Estresse Fisiológico/fisiologia , Sistema de Translocação de Argininas Geminadas/genética , Membrana Celular/metabolismo , Deleção de Genes , Perfilação da Expressão Gênica , Periplasma/metabolismo , Transporte Proteico/fisiologia , Proteoma/metabolismo
5.
Int J Mol Sci ; 19(9)2018 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-30205552

RESUMO

Vitamin D is a steroid-like hormone which acts by binding to vitamin D receptor (VDR). It plays a main role in the calcium homeostasis and metabolism. In addition, vitamin D display other important effects called "non-classical actions." Among them, vitamin D regulates immune cells function and hematopoietic cells differentiation and proliferation. Based on these effects, it is currently being evaluated for the treatment of hematologic malignancies. In addition, vitamin D levels have been correlated with patients' outcome after allogeneic stem cell transplantation, where it might regulate immune response and, accordingly, might influence the risk of graft-versus-host disease. Here, we present recent advances regarding its clinical applications both in the treatment of hematologic malignancies and in the transplant setting.

6.
Ann Hematol ; 97(3): 533-535, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29151134
8.
Educ. med. super ; 31(2): 0-0, abr.-jun. 2017. graf, tab
Artigo em Espanhol | LILACS | ID: biblio-891179

RESUMO

Introducción: una de las herramientas que aumenta tanto el interés como el valor de la tarea es el uso del mapa conceptual, que utiliza la representación visual de conceptos relacionados entre sí a través de interconectores. Objetivo: comparar si el uso de estos mapas conceptuales mejora el rendimiento académico y si existen diferencias en el rendimiento en relación al turno académico asignado. Métodos: para ello se seleccionaron de manera aleatoria a 200 alumnos de Grado de Enfermería (Centro Universitario de San Rafael Nebrija-Madrid-España). Resultados: los análisis realizados indican que las medias en cuanto al rendimiento académico son más elevadas en el grupo de alumnos que usaron mapas conceptuales, encontrándose diferencias significativas respecto a los que no lo usaron (t= -2,43; gl: 198; p> ,05). Por otro lado, se rechazó la influencia del turno asignado sobre el rendimiento académico. Conclusiones: el uso de los mapas conceptuales produce un efecto positivo en el rendimiento académico, lo que nos plantea la necesidad de realizar una docencia teórico-práctica más centrada en este método de aprendizaje(AU)


Introduction: One of the tools that increases both interest and value of the task is the use of conceptual maps, which uses the visual representation of concepts related to each other through interconnectors. Objective: To compare whether the use of these conceptual maps improves the academic performance and if there are differences in the performance in relation to the assigned academic shift. Methods: 200 students for the Nursing Degree (University Center of San Rafael Nebrija, Madrid, Spain) were randomly chosen. Results: The analyses carried out indicate that the means in terms of academic performance are higher in the group of students who used conceptual maps, finding significant differences in relationship to those who did not use them (t= -2.43; gl: 198; p> .05). On the other hand, the influence of the assigned shift on academic performance was rejected. Conclusions: The use of conceptual maps produces a positive effect on the academic performance, which raises the need for a theoretical-practical teaching centered on this method of learning(AU)


Assuntos
Desempenho Acadêmico , Estudo de Prova de Conceito , Estudantes de Enfermagem
9.
Leuk Lymphoma ; 58(8): 1893-1902, 2017 08.
Artigo em Inglês | MEDLINE | ID: mdl-27951729

RESUMO

Chronic medical diseases, evaluated by several comorbidities indexes have been reported to influence on overall survival in patients with myelodysplastic syndrome (MDS). However, these studies included patients with lower and higher-risk disease by IPSS. This study retrospectively evaluates the role of comorbidities (evaluated by the MDS comorbidity index; MDS-CI) together with clinical parameters in a series of 232 patients with LR-MDS (defined as either an IPSS score of low/intermediate-1 and favorable cytogenetic categories by IPSS-R). In multivariate analysis, together with age >75 years, diabetes requiring therapy and hemoglobin <10 g/dL; the incorporation of comorbidities by the MDS-CI (HR = 2.5; p< 0.0001) were independently associated to the probability of nonleukemic death (NLD). The combination of these variables allowed development of a model, which categorizes patients in three different groups with significantly different probability of NLD overtime (p< 0.001). This integrated score confirms the importance of comorbidities at diagnosis of patients with LR-MDS.


Assuntos
Síndromes Mielodisplásicas/diagnóstico , Síndromes Mielodisplásicas/epidemiologia , Idoso , Idoso de 80 Anos ou mais , Biomarcadores , Medula Óssea/patologia , Causas de Morte , Comorbidade , Feminino , Humanos , Incidência , Contagem de Leucócitos , Masculino , Pessoa de Meia-Idade , Síndromes Mielodisplásicas/mortalidade , Prevalência , Prognóstico , Estudos Retrospectivos , Risco , Análise de Sobrevida , Avaliação de Sintomas
10.
Clin Cancer Res ; 22(23): 5673-5681, 2016 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-27358490

RESUMO

PURPOSE: We describe the results of a prospective multicenter phase I/II trial evaluating the impact of the use of vitamin D (VitD) from day -5 to +100 on the outcome of patients undergoing allogeneic transplantation (EudraCT: 2010-023279-25; ClinicalTrials.gov: NCT02600988). EXPERIMENTAL DESIGN: A total of 150 patients were included in three consecutive cohorts of 50 patients each group: control group (CG, not receive VitD); low-dose group (LdD, received 1,000 IU VitD daily); and high-dose group (HdD, 5,000 IU VitD daily). We measured levels of VitD, cytokines, and immune subpopulations after transplantation. RESULTS: No significant differences were observed in terms of cumulative incidence of overall and grades 2-4 acute GVHD in terms of relapse, nonrelapse mortality, and overall survival. However, a significantly lower cumulative incidence of both overall and moderate plus severe chronic GVHD (cGVHD) at 1 year was observed in LdD (37.5% and 19.5%, respectively) and HdD (42.4% and 27%, respectively) as compared with CG (67.5% and 44.7%, respectively; P < 0.05). In multivariable analysis, treatment with VitD significantly decreased the risk of both overall (for LdD: HR = 0.31, P = 0.002; for HdD: HR = 0.36, P = 0.006) and moderate plus severe cGVHD (for LdD: HR = 0.22, P = 0.001; for HdD: HR = 0.33, P = 0.01). VitD modified the immune response, decreasing the number of B cells and naïve CD8 T cells, with a lower expression of CD40L. CONCLUSIONS: This is the first prospective trial that analyzes the effect of VitD postransplant. We observed a significantly lower incidence of cGVHD among patients receiving VitD. Interestingly, VitD modified the immune response after allo-SCT. Clin Cancer Res; 22(23); 5673-81. ©2016 AACR.


Assuntos
Doença Enxerto-Hospedeiro/imunologia , Fatores Imunológicos/imunologia , Vitamina D/imunologia , Adolescente , Adulto , Feminino , Transplante de Células-Tronco Hematopoéticas/métodos , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Transplante Homólogo/métodos , Adulto Jovem
11.
Acta investigación psicol. (en línea) ; 6(2): 2459-2468, ago. 2016. graf
Artigo em Espanhol | LILACS-Express | ID: biblio-949437

RESUMO

Resumen El objetivo del presente estudio fue analizar la influencia del apoyo social de la pareja en el ajuste a la enfermedad cardiaca, así como su influencia en la adhesión a las pautas clínicas. La muestra estuvo compuesta por 109 pacientes con sus parejas. Se llevaron a cabo las mediciones mientras realizaban el Programa de Rehabilitación Cardiaca del Hospital Ramón y Cajal de Madrid. Los resultados indican que la pareja no es una fuente de apoyo relevante para el ajuste a la enfermedad del paciente (apoyo recibido [p = 0.46] y satisfacción con el apoyo recibido [p = 0.41]. Sin embargo, el apoyo de la pareja sí resulta especialmente importante de cara al cumplimiento de hábitos cardiosaludables, sobre todo en el seguimiento realizado a los 8 y a los 12 meses (p < 0.001), lo que conduce a analizar la posibilidad de que la pareja no sea importante para el ajuste a la enfermedad debido posiblemente a que la muestra está pasando por una intervención clínica y, por tanto, es posible que otras fuentes como profesionales de la salud u otros pacientes estén ejerciendo un papel predominante de apoyo. En el momento en que esta supervisión clínica desaparece, el apoyo de la pareja es de vital importancia para la adhesión a las pautas recomendadas, las cuales tienen relación con cambios en el estilo de vida y, por tanto, en la prevención de recaídas.


Abstract The main aim of our study is to analyze the influence of social support on cardiac illness adjustment and on health recommendations adherence. The sample was 109 patients and their partners, all of them included in the Cardiac Rehabilitation Program of the Ramon y Cajal Hospital in Madrid. Our results show that partner support has not a significant impact on patients¿ adjustment (received support [P = .46] and satisfaction with received support [P = .041]). However, partner support has a significant effect on adherence to health recommendations (food, physical exercise, smoking, etc), most of all in the 8 and 12 months' follow-up (P < .001). Perhaps, in the first stages of illness, support provided for other sources (health professionals, other patients, etc.) could be more important than partner support, most of all taking into account that patients were included in a clinical intervention. As supervision decreases, the effect of partner support on adherence increases. The implications of our results for future interventions and research are discussed.

12.
Acta Haematol ; 134(2): 71-5, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25895602

RESUMO

We report on the emergence and clinical relevance of an unusual BCR-ABL1 kinase domain mutational status in a 2-year-old female with p210-BCR-ABL Philadelphia chromosome-positive acute lymphoblastic leukaemia. We detected three BCR-ABL1 clones determined by the presence of the E255V, D276G and F317L mutations. We point out the usefulness of searching for mutated populations that survive tyrosine-kinase inhibitor therapy and the role of their clonal selection over time in relation to therapeutic intervention.


Assuntos
Antineoplásicos/uso terapêutico , Resistencia a Medicamentos Antineoplásicos , Proteínas de Fusão bcr-abl/genética , Cromossomo Filadélfia , Leucemia-Linfoma Linfoblástico de Células Precursoras/tratamento farmacológico , Leucemia-Linfoma Linfoblástico de Células Precursoras/genética , Inibidores de Proteínas Quinases/uso terapêutico , Substituição de Aminoácidos , Transplante de Medula Óssea , Pré-Escolar , Células Clonais , Terapia Combinada , Monitoramento de Medicamentos , Resistência a Múltiplos Medicamentos , Evolução Fatal , Feminino , Proteínas de Fusão bcr-abl/antagonistas & inibidores , Proteínas de Fusão bcr-abl/metabolismo , Humanos , Transfusão de Linfócitos , Leucemia-Linfoma Linfoblástico de Células Precursoras/patologia , Leucemia-Linfoma Linfoblástico de Células Precursoras/terapia , Recidiva , Transplante Homólogo
13.
Clin Lymphoma Myeloma Leuk ; 15(4): 236-44, 2015 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-25487600

RESUMO

BACKGROUND: The prognosis of myelodysplastic syndromes (MDS) and acute myeloid leukaemia (AML) is very heterogeneous. PATIENTS AND METHODS: We analyzed the prognostic value of several genes in a cohort of 85 MDS and AML patients. RESULTS: Overexpression of glycogen synthase 1 and macrophage migration inhibitory factor genes had an adverse outcome in multivariate analysis (P = .003 and P < .001, respectively). Furthermore, the higher expression of myelocytomatosis oncogene was associated with a lower response to azacitidine (P = .03). CONCLUSION: In the current study we identified a specific gene expression profile as prognostic factors for response to azacitidine and survival in MDS and AML.


Assuntos
Expressão Gênica , Genes myc , Glicogênio Sintase/genética , Oxirredutases Intramoleculares/genética , Leucemia Mieloide Aguda/tratamento farmacológico , Leucemia Mieloide Aguda/genética , Fatores Inibidores da Migração de Macrófagos/genética , Síndromes Mielodisplásicas/tratamento farmacológico , Síndromes Mielodisplásicas/genética , Idoso , Idoso de 80 Anos ou mais , Antimetabólitos Antineoplásicos/uso terapêutico , Azacitidina/uso terapêutico , Perfilação da Expressão Gênica , Humanos , Hipóxia/genética , Leucemia Mieloide Aguda/diagnóstico , Leucemia Mieloide Aguda/mortalidade , Pessoa de Meia-Idade , Síndromes Mielodisplásicas/diagnóstico , Síndromes Mielodisplásicas/mortalidade , Prognóstico , Análise de Sobrevida , Resultado do Tratamento
14.
Span. j. psychol ; 17(1): e10.1-e10.8, ene.-dic. 2014. tab
Artigo em Inglês | IBECS | ID: ibc-130519

RESUMO

This study analyzes the effect on levels of patient anxiety and depression of a partner joining a cardiac rehabilitation program support group, also taking into account the sex of the patient. The study was undertaken using a two-group comparison design with pre-and post-test measures in non-equivalent groups. The sample comprised patients in the cardiac rehabilitation program (CRP) at the Ramón y Cajal Hospital, Madrid (Spain). Analysis of covariance (ANCOVA) showed direct effects of sex and partner participation in support groups on the anxiety trait. Similarly, interaction effects were observed between the sex variable and partner participation. These results indicate the pertinence of designing separate groups for patients and partners (AU)


No disponible


Assuntos
Humanos , Masculino , Feminino , Ansiedade/psicologia , Escala de Ansiedade Frente a Teste/normas , Depressão/psicologia , Doenças Cardiovasculares/psicologia , Doenças Cardiovasculares/reabilitação , Psicologia Industrial/educação , Psicologia Industrial/métodos , Resultado do Tratamento , Anormalidades Cardiovasculares/psicologia , Análise de Variância , Psicologia Industrial/normas
15.
Span J Psychol ; 17: E10, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25011462

RESUMO

This study analyzes the effect on levels of patient anxiety and depression of a partner joining a cardiac rehabilitation program support group, also taking into account the sex of the patient. The study was undertaken using a two-group comparison design with pre-and post-test measures in non-equivalent groups. The sample comprised patients in the cardiac rehabilitation program (CRP) at the Ramón y Cajal Hospital, Madrid (Spain). Analysis of covariance (ANCOVA) showed direct effects of sex and partner participation in support groups on the anxiety trait. Similarly, interaction effects were observed between the sex variable and partner participation. These results indicate the pertinence of designing separate groups for patients and partners.


Assuntos
Ansiedade/psicologia , Depressão/psicologia , Cardiopatias/reabilitação , Grupos de Autoajuda , Cônjuges/psicologia , Adulto , Idoso , Feminino , Cardiopatias/psicologia , Humanos , Masculino , Pessoa de Meia-Idade
16.
Clin Lymphoma Myeloma Leuk ; 14(1): 80-6, 2014 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-24220615

RESUMO

Incidence, etiology, and outcome of infectious episodes in patients with myeloid neoplasms receiving azacitidine are uncertain, with no prospective data available in this group of patients. The aim of the current study was to analyze the incidence and factors related to the probability of infection in a cohort of patients with myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML) treated with azacitidine who did not receive any type of antimicrobial prophylaxis. Significantly, the group of patients who received prior intensive chemotherapy had more infectious episodes (P = 10(-4)), and particularly, invasive aspergillosis (P = .015), than patients who received frontline azacitidine. Primary antifungal prophylaxis might be recommended in MDS and AML patients receiving azacitidine as salvage therapy after intensive regimens.


Assuntos
Antifúngicos/uso terapêutico , Antimetabólitos Antineoplásicos/uso terapêutico , Azacitidina/uso terapêutico , Leucemia Mieloide Aguda/tratamento farmacológico , Leucemia Mieloide Aguda/microbiologia , Síndromes Mielodisplásicas/tratamento farmacológico , Síndromes Mielodisplásicas/microbiologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Humanos , Incidência , Pessoa de Meia-Idade , Estudos Retrospectivos , Terapia de Salvação/métodos
17.
J Women Aging ; 25(4): 358-71, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24116995

RESUMO

The purpose of this study is to estimate the prevalence of lifetime intimate partner violence (IPV) in older women and to analyze its effect on women's health and Healthcare Services utilization. Women aged 55 years and over (1,676) randomly sampled from Primary Healthcare Services around Spain were included. Lifetime IPV prevalence, types, and duration were calculated. Descriptive and multivariate procedures using logistic and multiple lineal regression models were used. Of the women studied, 29.4% experienced IPV with an average duration of 21 years. Regardless of the type of IPV experienced, abused women showed significantly poorer health and higher healthcare services utilization compared to women who had never been abused. The high prevalence detected long standing duration, negative health impact, and high healthcare services utilization, calling attention to a need for increased efforts aimed at addressing IPV in older women.


Assuntos
Serviços de Saúde/estatística & dados numéricos , Maus-Tratos Conjugais/psicologia , Maus-Tratos Conjugais/estatística & dados numéricos , Saúde da Mulher/estatística & dados numéricos , Idoso , Analgésicos/uso terapêutico , Antidepressivos/uso terapêutico , Estudos Transversais , Feminino , Nível de Saúde , Humanos , Pessoa de Meia-Idade , Prevalência , Espanha/epidemiologia , Inquéritos e Questionários , Tranquilizantes/uso terapêutico
18.
Clin Lymphoma Myeloma Leuk ; 13(2): 144-52, 2013 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-23137720

RESUMO

UNLABELLED: Prognosis of myelodysplastic syndromes (MDS) is an area of ongoing interest. Identification of patients with poor outcome in the categories of lower risk disease is critical. In this study, we classify a cohort of 332 lower risk MDS into 3 groups with differences in survival and risk for leukemic progression that could drive treatment approaches to improve prognosis in a fraction of these patients. BACKGROUND: Prognosis of MDS and particularly in patients categorized as lower risk (< 10% blasts or low and intermediate-1 International Prognostic Scoring System [IPSS]) is very heterogeneous and includes patients with very different outcomes with current scoring systems. Recently, a new cytogenetic classification has been proposed for the revised IPSS in predicting the outcome for MDS. PATIENTS AND METHODS: To evaluate the prognostic significance of multiple variables for survival and risk of progression to acute myeloid leukemia, we analyzed baseline characteristics of 332 lower risk MDS patients within the lower risk cytogenetic categories by IPSS and the recent proposal for the new cytogenetic classification. RESULTS: In multivariate analysis, severity of cytopenias, age > 60 years, bone marrow blasts (5%-9%) and transfusion dependency significantly influenced outcome. The combination of these variables allowed development of a model which categorizes patients in 3 different groups with median survival of 95, 44, and 13 months for groups 1, 2, and 3, respectively (P < .001). In addition, this score also stratified patients for their risk for leukemic progression, estimated at 2 years in 3.1%, 7.6%, and 21.3% for each group (P = .024). CONCLUSION: Although karyotype remains the main prognostic factor in MDS, the current study identifies clinical parameters predicting outcome among patients with the better cytogenetic profile. Degree of cytopenias, blasts 5%-9% and transfusion dependence might identify a subset of patients within the nonadverse karyotype, in which early or more aggressive approaches could possibly be required to improve survival or prevent disease progression.


Assuntos
Células da Medula Óssea/patologia , Aberrações Cromossômicas , Leucemia Mieloide Aguda/etiologia , Leucemia Mieloide Aguda/mortalidade , Síndromes Mielodisplásicas/diagnóstico , Síndromes Mielodisplásicas/mortalidade , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Progressão da Doença , Feminino , Humanos , Cariótipo , Masculino , Pessoa de Meia-Idade , Prognóstico , Adulto Jovem
19.
J Biomed Mater Res A ; 100(6): 1615-22, 2012 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-22447651

RESUMO

The surface properties of porous silicon (PSi) evolve rapidly in phosphate-buffered saline. X-ray photoelectron spectra indicate the formation of a Si-OH and C-O enriched surface, which becomes increasingly hydrophilic with aging time. Multiscale stripe micropatterns of Si and PSi have been fabricated by means of a high-energy ion-beam irradiation process. These micropatterns have been aged in physiological conditions and used to analyze human mesenchymal stem cell (hMSC) adhesion. The actin cytoskeleton of hMSCs orients following the uniaxial micropatterns. In the wider Si stripes, hMSCs are dominantly located on Si areas. However, for reduced Si widths, adhesion is avoided on PSi by a split assembly of the actin cytoskeleton on two parallel Si areas. These results confirm that nanostructured Si-OH/C-O-rich surfaces with hydrophilic character are specially adapted for the creation of cell adhesion surface contrasts.


Assuntos
Materiais Biocompatíveis/química , Silício/química , Adesão Celular , Células Cultivadas , Humanos , Interações Hidrofóbicas e Hidrofílicas , Células-Tronco Mesenquimais/citologia , Nanoestruturas/química , Porosidade , Propriedades de Superfície
20.
J Epidemiol Community Health ; 66(4): 352-8, 2012 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21177662

RESUMO

BACKGROUND: Research on women''s responses to intimate partner violence (IPV) has largely been limited to women who have been exposed to severe physical violence with scarce generalisation. This study aimed to analyse how Spanish abused women from different backgrounds and with different IPV characteristics respond to violence. METHOD: Women experiencing IPV before the previous year (1469) were selected from a large cross-sectional national survey of adult women recruited during 2006-7 among female patients seeking medical care for whatever reason in primary healthcare services. The outcome variables were women's responses to IPV and the predictor variables were personal and social resources profiles and characteristics of the abuse (type, duration and women's age at onset). Stepwise logistic regression models were fitted. RESULTS: 87.5% of abused women took some kind of action to overcome IPV. Significant differences on personal and social profile and type and duration of the abuse were detected between the three strategic responses: distancing, in process and inhibition. The probability of a woman responding with a distancing strategy (seeking outside help or leaving temporarily) is almost three times greater if she is employed, was young when the abuse began, had experienced physical and psychological abuse and when the abuse was under 5 years. CONCLUSIONS: The results of this study show that personal and social resources and the specific circumstances of the abuse should be taken into account to understand women's responses to IPV. Well-validated interventions targeted at abused women's needs and the circumstances of IPV remain a priority.


Assuntos
Mulheres Maltratadas/psicologia , Violência Doméstica/estatística & dados numéricos , Aceitação pelo Paciente de Cuidados de Saúde/psicologia , Atenção Primária à Saúde/estatística & dados numéricos , Parceiros Sexuais , Adulto , Mulheres Maltratadas/estatística & dados numéricos , Estudos Transversais , Violência Doméstica/prevenção & controle , Características da Família , Feminino , Pesquisa sobre Serviços de Saúde , Humanos , Modelos Logísticos , Saúde Mental , Pessoa de Meia-Idade , Gravidez , Autoimagem , Fatores Socioeconômicos , Espanha/epidemiologia , Inquéritos e Questionários , Saúde da Mulher/estatística & dados numéricos
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