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1.
PLoS One ; 14(10): e0223975, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31626639

RESUMO

The Salmonella enterica serovar Typhimurium sequence type 213 (ST213) emerged as a predominant genotype in Mexico. It is characterized by harboring multidrug resistance (MDR) IncC plasmids (previously IncA/C) and the lack of the Salmonella virulence plasmid (pSTV). Here we show that the D6-like plasmid prophage is present in most of the ST213 strains. We used the reported nucleotide sequence of YU39 plasmid (pYU39_89) to design a PCR typing scheme for the D6-like plasmid prophages, and determined the complete nucleotide sequences for the D6-like prophages of three additional ST213 strains (YU07-18, SL26 and SO21). Two prophage variants were described: i) a complete prophage, containing homologous sequences for most of the genetic modules described in P1 and D6 phages, which most likely allow for the lytic and lysogenic lifestyles; and ii) an incomplete prophage, lacking a 15 kb region containing morphogenesis genes, suggesting that it is defective. The tail fiber gene inversion region was the most divergent one between D6 and pYU39_89 genomes, suggesting the production of a distinct set of tail fibers, which could be involved in host range preferences. A glutaminyl-tRNA synthetase gene (glnS), which could be involved in providing host cell increased fitness or plasmid maintenance functions, was found in all D6-like genomes. Population level analysis revealed a biogeographic pattern of distribution of these plasmid-phages and specific associations with variants of MDR IncC plasmids. Statistically significant associations were found between the two prophage variants (p75 or p89), the type of IncC plasmids (I or II) and geographic isolation regions (Sonora, San Luis Potosí, Michoacán and Yucatán). This work integrates results from molecular typing, genomics and epidemiology to provide a broad overview for the evolution of an emergent Salmonella genotype.


Assuntos
Plasmídeos/metabolismo , Prófagos/fisiologia , Salmonella typhimurium/patogenicidade , Aminoacil-tRNA Sintetases/genética , Evolução Molecular , Genoma Viral , Genômica/métodos , Genótipo , Plasmídeos/genética , Reação em Cadeia da Polimerase , Prófagos/genética , Prófagos/isolamento & purificação , Salmonella typhimurium/genética , Salmonella typhimurium/virologia , Proteínas Virais/genética , Virulência/genética
2.
Suma psicol ; 24(1): 25-33, ene.-jun. 2017. tab
Artigo em Inglês | LILACS-Express | ID: biblio-904057

RESUMO

ABSTRACT This study aimed to characterize dieting and non-dieting university students by gender, Received 14 September 2016 based on their satisfaction with life and their food-related life, self-discrepancy, food behavior and health-related aspects. A non-probabilistic sample of 305 students from five Chilean state universities responded a questionnaire that included the Revised Restraint Scale, the Satisfaction with Life Scale, the Satisfaction with Food-related Life Scale, the Health-related Quality of Life Index, the Nutrition Interest Scale and the Self-Discrepancy Index. Sociodemographic characteristics, food behavior, and approximate weight and height were also enquired. Chronic dieters and non-dieters were distinguished according to the median score of the Revised Restraint Scale. 51.1% of women and 55.5% of men classified as chronic dieters, sharing characteristics such as nutrition concern, mental health problems, higher body mass index, and physical and economic self-discrepancy. Women dieters reported lower life satisfaction and satisfaction with food-related life, more health problems and health-related restriction of food, while men dieters showed higher social and emotional self-discrepancy.


RESUMEN El objetivo de este estudio fue caracterizar a estudiantes universitarios dietantes y no dietantes de distinto género, según su satisfacción con la vida y con la alimentación, discrepancia del yo, comportamiento alimentario y aspectos relacionados con la salud. Se aplicó un cuestionario a una muestra no probabilística de 305 estudiantes de cinco universidades estatales de Chile. El cuestionario incluyó la Escala Revisada de Restricción Alimentaria, la Escala de Satisfacción con la Vida, la Escala de Satisfacción con la Alimentación, el Índice de Calidad de Vida relativo a la Salud, la Escala de Interés por la Nutrición y la Escala de Discrepancia del Yo. Se consultaron características sociodemográficas, comportamiento alimentario y el peso y estatura aproximados. Con base en la mediana de la Escala Revisada de Restricción Alimentaria se distinguieron dietantes crónicos y no dietantes. El 51.1% de las mujeres y el 55.5% de los hombres fueron clasificados como dietantes crónicos, quienes comparten características como la preocupación por la nutrición, problemas de salud mental, mayor índice de masa corporal, y discrepancia del yo en los ámbitos físico y económico. Las mujeres dietantes estuvieron menos satisfechas con su vida y con su alimentación, reportaron más problemas de salud y superior restricción de alimentos por motivos de salud. Los hombres dietantes mostraron mayor discrepancia del yo en los ámbitos social y emocional.

3.
Eat Behav ; 21: 123-8, 2016 04.
Artigo em Inglês | MEDLINE | ID: mdl-26835591

RESUMO

Self-discrepancy describes the distance between an ideal and the actual self. Research suggests that self-discrepancy and dietary restraint are related, causing a significant impact on the person's well-being. However, this relationship has been mostly reported in female and mixed populations. In order to further explore dietary behaviors and their relations to self-discrepancy and well-being-related variables in men, a survey was applied to a non-probabilistic sample of 119 male students from five Chilean state universities (mean age=21.8, SD=2.75). The questionnaire included the Revised Restraint Scale (RRS) with the subscales weight fluctuations (WF) and diet concern (DC), the Satisfaction with Life Scale (SWLS), the Satisfaction with Food-Related Life Scale (SWFL), the Nutrition Interest Scale (NIS), and the Self-discrepancy Index (SDI). Questions were asked about socio-demographic characteristics, eating and drinking habits, and approximate weight and height. A cluster analysis applied to the Z-scores of the RRS classified the following typologies: Group 1 (22.7%), men concerned about weight fluctuations; Group 2 (37.0%), men concerned about diet and weight fluctuations; Group 3 (40.3%), unconcerned about diet and weight fluctuations. The typologies differed in their SDI score, restriction on pastry consumption and reported body mass index (BMI). Students with higher DC and WF scores had a higher BMI, and tended to report high self-discrepancy not only on a physical level, but also on social, emotional, economic and personal levels. This study contributes to the literature on subjective well-being, dietary restraint and self-discrepancy in men from non-clinical samples.


Assuntos
Peso Corporal , Dieta Redutora/psicologia , Comportamento Alimentar , Satisfação Pessoal , Estudantes/psicologia , Inquéritos e Questionários , Universidades , Escala de Avaliação Comportamental , Estatura , Índice de Massa Corporal , Chile , Análise por Conglomerados , Emoções , Alimentos , Humanos , Masculino , Ciências da Nutrição , Adulto Jovem
4.
BMC Microbiol ; 16: 18, 2016 Feb 09.
Artigo em Inglês | MEDLINE | ID: mdl-26862033

RESUMO

BACKGROUND: Classical strains of Salmonella enterica serovar Typhimurium (Typhimurium) predominantly cause a self-limiting diarrheal illness in humans and a systemic disease in mice. In this study, we report the characterization of a strain isolated from a blood-culture taken from a 15-year old woman suffering from invasive severe salmonellosis, refractory to conventional therapy with extended-spectrum cephalosporin (ESC). RESULTS: The strain, named 33676, was characterized as multidrug-resistant Salmonella serogroup A by biochemical, antimicrobial and serological tests. Multilocus sequence typing (MLST) and XbaI macrorestrictions (PFGE) showed that strain 33676 belonged to the Typhimurium ST213 genotype, previously described for other Mexican Typhimurium strains. PCR analyses revealed the presence of IncA/C, IncFIIA and ColE1-like plasmids and the absence of the Salmonella virulence plasmid (pSTV). Conjugation assays showed that the ESC-resistance gene bla CMY-2 was carried on the conjugative IncF plasmid, instead of the IncA/C plasmid, as found in previously studied ST213 strains. Although the IncA/C plasmid conferred most of the observed antimicrobial resistances it was not self-conjugative; it was rather able to conjugate by co-integrating with the IncF plasmid. Strain 33676 was fully attenuated for virulence in BALB/c mice infections. Both type-three secretion system (T3SS), encoded in Salmonella pathogenicity islands 1 and 2 (SPI-1 and SPI-2), were functional in the 33676 strain and, interestingly, this strain produced the H2 FljB flagellin instead of the H1 FliC flagellin commonly expressed by S. enterica strains. CONCLUSIONS: Strain 33676 showed two main features that differentiate it from the originally described ST213 strains: 1) the bla CMY-2 gene was not carried on the IncA/C plasmid, but on a conjugative IncF plasmid, which may open a new route of dissemination for this ESC-resistance gene, and 2) it expresses the H2 FljB flagella, in contrast with the other ST213 and most Typhimurium reference strains. To our knowledge this is the first report of an IncF bla CMY-2-carrying plasmid in Salmonella.


Assuntos
Farmacorresistência Bacteriana Múltipla , Plasmídeos/genética , Infecções por Salmonella/microbiologia , Salmonella typhimurium/enzimologia , Salmonella typhimurium/patogenicidade , beta-Lactamases/metabolismo , Animais , Antibacterianos/farmacologia , Feminino , Humanos , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Plasmídeos/metabolismo , Salmonella typhimurium/efeitos dos fármacos , Salmonella typhimurium/genética , Virulência , beta-Lactamases/genética
5.
Genome Announc ; 3(6)2015 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-26564044

RESUMO

Salmonella enterica subsp. enterica serovar Typhimurium strain 33676 was isolated in Mexico City, Mexico, from a patient with a systemic infection, and its complete genome sequence was determined using PacBio single-molecule real-time technology. Strain 33676 harbors an IncF plasmid carrying the extended-spectrum cephalosporin gene blaCMY-2 and a multidrug resistance IncA/C plasmid.

6.
Int Microbiol ; 18(2): 99-104, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-26496617

RESUMO

In this work, IS200 and multi-locus sequence typing (MLST) were used to analyze 19 strains previously serotyped as Salmonella enterica serovar Typhi and isolated in Indonesia (16 strains), Mexico (2 strains), and Switzerland (1 strain). Most of the strains showed the most common Typhi sequence types, ST1 and ST2, and a new Typhi genotype (ST1856) was described. However, one isolate from Mexico and another from Indonesia were of the ST365 and ST426 sequence types, indicating that they belonged to serovars Weltevreden and Aberdeen, respectively. These results were supported by the amplification of IS200 fragments, which rapidly distinguish Typhi from other serovars. Our results demonstrate the utility of IS200 and MLST in the classification of Salmonella strains into serovars. These methods provide information on the clonal relatedness of strains isolated worldwide.


Assuntos
Tipagem de Sequências Multilocus/métodos , Reação em Cadeia da Polimerase/métodos , Infecções por Salmonella/microbiologia , Salmonella typhi/isolamento & purificação , Sequência de Bases , Humanos , Indonésia , Dados de Sequência Molecular , Filogenia , Salmonella typhi/classificação , Salmonella typhi/genética
7.
Front Microbiol ; 6: 807, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26300871

RESUMO

Salmonella enterica can cause intestinal or systemic infections in humans and animals mainly by the presence of pathogenicity islands SPI-1 and SPI-2, containing 39 and 44 genes, respectively. The AraC-like regulator HilD positively controls the expression of the SPI-1 genes, as well as many other Salmonella virulence genes including those located in SPI-2. A previous report indicates that the two-component system CpxR/A regulates the SPI-1 genes: the absence of the sensor kinase CpxA, but not the absence of its cognate response regulator CpxR, reduces their expression. The presence and absence of cell envelope stress activates kinase and phosphatase activities of CpxA, respectively, which in turn controls the level of phosphorylated CpxR (CpxR-P). In this work, we further define the mechanism for the CpxR/A-mediated regulation of SPI-1 genes. The negative effect exerted by the absence of CpxA on the expression of SPI-1 genes was counteracted by the absence of CpxR or by the absence of the two enzymes, AckA and Pta, which render acetyl-phosphate that phosphorylates CpxR. Furthermore, overexpression of the lipoprotein NlpE, which activates CpxA kinase activity on CpxR, or overexpression of CpxR, repressed the expression of SPI-1 genes. Thus, our results provide several lines of evidence strongly supporting that the absence of CpxA leads to the phosphorylation of CpxR via the AckA/Pta enzymes, which represses both the SPI-1 and SPI-2 genes. Additionally, we show that in the absence of the Lon protease, which degrades HilD, the CpxR-P-mediated repression of the SPI-1 genes is mostly lost; moreover, we demonstrate that CpxR-P negatively affects the stability of HilD and thus decreases the expression of HilD-target genes, such as hilD itself and hilA, located in SPI-1. Our data further expand the insight on the different regulatory pathways for gene expression involving CpxR/A and on the complex regulatory network governing virulence in Salmonella.

8.
Suma psicol ; 22(2): 120-128, jul.-dic. 2015. ilus, tab
Artigo em Inglês | LILACS-Express | ID: lil-779705

RESUMO

This study aimed to identify the factors determining the happiness in young and middleaged adults in Chile, including health and food-related factors, and their relationships between them. To achieve this objective, a survey was conducted on a total of 1163 people (from 20 to 65 years old), proportionally distributed in the cities over 100000 inhabitants in the central area of Chile. The main scales used were: Subjective Happiness Scale (SHS), Health Related Quality of Life Index (HRQoL), and Satisfaction with Food-related Life (SWFL). In this study, the logistic ordinal regression models were estimated. The mainfinding was that people have more unhealthy days, poorer perception of their health, and are less satisfied with their food-related life, and are less happy in both age groups. Therefore, health-related factors and SWFL are strong predictors of happiness.


En este estudio se identifican los determinantes de la felicidad en adultos jóvenes y personas de edad media en Chile, incluyendo factores relacionados con la salud y la alimentación, y las conexiones entre ellos. Un total de 1163 personas fueron entrevistadas (entre 20 y 65 años de edad), distribuidas proporcionalmente por las ciudades de más de 100000 habitantes de la zona central de Chile. Las principales escalas utilizadas fueron: Escala de Felicidad Subjetiva, Calidad de Vida Relacionada con la Salud, y Satisfacción con la Vida Relacionada con los Alimentos. En este estudio se estimaron modelos de regresión logística ordinal. El principal hallazgo es que las personas con más días no saludables, más pobre percepción de su estado de salud, y menos satisfechas con su vida relacionada con los alimentos, son menos felices en ambos grupos de edad. Esto significa que los factores relacionados con la salud y la satisfacción con la vida relacionada con los alimentos son fuertes predictores de la felicidad.

9.
Nutr Hosp ; 31(6): 2752-63, 2015 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-26040392

RESUMO

AIM: To characterize types of university students based on satisfaction with life domains that affect eating habits, satisfaction with food-related life and subjective happiness. MATERIALS AND METHODS: A questionnaire was applied to a nonrandom sample of 305 students of both genders in five universities in Chile. The questionnaire included the abbreviated Multidimensional Student's Life Satisfaction Scale (MSLSS), Satisfaction with Food-related Life Scale (SWFL) and the Subjective Happiness Scale (SHS). Eating habits, frequency of food consumption in and outside the place of residence, approximate height and weight and sociodemographic variables were measured. RESULTS: Using factor analysis, the five-domain structure of the MSLSS was confirmed with 26 of the 30 items of the abbreviated version: Family, Friends, Self, Environment and University. Using cluster analysis four types of students were distinguished that differ significantly in the MSLSS global and domain scores, SWFL and SHS scores, gender, ownership of a food allowance card funded by the Chilean government, importance attributed to food for well-being and socioeconomic status. CONCLUSIONS: Higher levels of life satisfaction and happiness are associated with greater satisfaction with food-related life. Other major life domains that affect students' subjective well-being are Family, Friends, University and Self. Greater satisfaction in some domains may counterbalance the lower satisfaction in others.


Assuntos
Alimentos , Felicidade , Satisfação Pessoal , Estudantes/psicologia , Chile , Comportamento Alimentar , Feminino , Humanos , Masculino , Psicometria , Fatores Socioeconômicos , Inquéritos e Questionários , Adulto Jovem
10.
Genome Announc ; 3(3)2015 Jun 18.
Artigo em Inglês | MEDLINE | ID: mdl-26089426

RESUMO

Salmonella enterica subsp. enterica serovar Typhimurium strain YU39 was isolated in 2005 in the state of Yucatán, Mexico, from a human systemic infection. The YU39 strain is representative of the multidrug-resistant emergent sequence type 213 (ST213) genotype. The YU39 complete genome is composed of a chromosome and seven plasmids.

11.
Span J Psychol ; 18: E38, 2015 Jun 17.
Artigo em Inglês | MEDLINE | ID: mdl-26083311

RESUMO

This study aimed to assess the effect of satisfaction with food-related life on life satisfaction among inhabitants of the main municipalities of central Chile. A survey was applied to a sample of 1,277 people, distributed proportionally by municipality. The questionnaire included the following scales: SWLS (Satisfaction with Life Scale), SWFL (Satisfaction with Food-related Life) and the Health-Related Quality of Life Index (HRQOL). Questions were asked regarding eating habits inside and outside the home, time available for meals at home, the assessment of five sources of happiness and the demographic characteristics of those surveyed. An ordered logit model was proposed, in which the dependent variable was satisfaction with life. Satisfaction with life was significantly related to the respondent's socioeconomic status, self-perception of health, degree of satisfaction with food-related life, monthly food expenditure, time available for supper with the family (p < .01); gender, self-reported number of days affected by mental health problems, frequency of supper with the family, the degree of agreement with respect to family being an important source of happiness (p < .05); and family size and frequency of food consumption in fast food outlets (p < .10). Satisfaction with life in the study sample is related to aspects associated with health, family and eating, and the family interaction associated with eating may play an important role in overall satisfaction with life.


Assuntos
Ingestão de Alimentos/etnologia , Família/etnologia , Alimentos , Satisfação Pessoal , Qualidade de Vida/psicologia , Adulto , Chile/etnologia , Humanos , Renda
12.
Nutr. hosp ; 31(6): 2752-2763, jun. 2015. tab
Artigo em Inglês | IBECS | ID: ibc-142266

RESUMO

Aim: to characterize types of university students based on satisfaction with life domains that affect eating habits, satisfaction with food-related life and subjective happiness. Materials and methods: a questionnaire was applied to a nonrandom sample of 305 students of both genders in five universities in Chile. The questionnaire included the abbreviated Multidimensional Student’s Life Satisfaction Scale (MSLSS), Satisfaction with Food-related Life Scale (SWFL) and the Subjective Happiness Scale (SHS). Eating habits, frequency of food consumption in and outside the place of residence, approximate height and weight and sociodemographic variables were measured. Results: using factor analysis, the five-domain structure of the MSLSS was confirmed with 26 of the 30 items of the abbreviated version: Family, Friends, Self, Environment and University. Using cluster analysis four types of students were distinguished that differ significantly in the MSLSS global and domain scores, SWFL and SHS scores, gender, ownership of a food allowance card funded by the Chilean government, importance attributed to food for well-being and socioeconomic status. Conclusions: higher levels of life satisfaction and happiness are associated with greater satisfaction with food-related life. Other major life domains that affect students’ subjective well-being are Family, Friends, University and Self. Greater satisfaction in some domains may counterbalance the lower satisfaction in others (AU)


Objetivo: caracterizar tipologías de estudiantes universitarios según la satisfacción en dominios de la vida que afectan a los hábitos alimentarios, satisfacción con la alimentación y nivel de felicidad subjetiva. Material y método: se aplicó un cuestionario a una muestra no probabilística de 305 estudiantes de ambos géneros de cinco universidades de Chile. El cuestionario incluyó: Escala Multidimensional de Satisfacción con la Vida para Estudiantes (MSLSS) abreviada, Satisfacción con la Alimentación (SWFL) y la Escala de Felicidad Subjetiva (SHS). Se consultaron hábitos de consumo de alimentos, frecuencia de comidas dentro y fuera del lugar de residencia, peso y estatura aproximada y variables sociodemográficas. Resultados: mediante análisis factorial se confirmó la estructura de cinco dominios de la MSLSS, con 26 de los 30 ítems de la versión abreviada: Familia, Amigos, Sí mismo, Entorno de vida y Universidad. Mediante análisis clúster se distinguieron cuatro tipologías que difirieron significativamente en los puntajes de los dominios de la MSLSS y en su puntaje global, en los puntajes de la SWFL, SHS, género, contar con una tarjeta de alimentación financiada por el Estado chileno, importancia asignada a la alimentación para el bienestar personal y nivel socioeconómico Conclusiones: mayores niveles de satisfacción con la vida y de felicidad se asocian con una mayor satisfacción con la alimentación. Otros dominios importantes que afectan al bienestar subjetivo de los estudiantes corresponden a la familia, los amigos, la universidad y el sí mismo. La mayor satisfacción en unos dominios compensaría la menor satisfacción en otros (AU)


Assuntos
Humanos , Felicidade , Comportamento Alimentar/psicologia , Comportamento Alimentar/psicologia , Psicometria/instrumentação , Estudantes/psicologia , Qualidade de Vida , Satisfação Pessoal
13.
Int. microbiol ; 18(2): 99-104, jun. 2015. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-143387

RESUMO

In this work, IS200 and multi-locus sequence typing (MLST) were used to analyze 19 strains previously serotyped as Salmonella enterica serovar Typhi and isolated in Indonesia (16 strains), Mexico (2 strains), and Switzerland (1 strain). Most of the strains showed the most common Typhi sequence types, ST1 and ST2, and a new Typhi genotype (ST1856) was described. However, one isolate from Mexico and another from Indonesia were of the ST365 and ST426 sequence types, indicating that they belonged to serovars Weltevreden and Aberdeen, respectively. These results were supported by the amplification of IS200 fragments, which rapidly distinguish Typhi from other serovars. Our results demonstrate the utility of IS200 and MLST in the classification of Salmonella strains into serovars. These methods provide information on the clonal relatedness of strains isolated worldwide (AU)


No disponible


Assuntos
Humanos , Salmonella enterica/isolamento & purificação , Tipagem de Sequências Multilocus/métodos , Febre Tifoide/imunologia , Tipagem Molecular/métodos , Mutagênese Insercional/imunologia
14.
Appetite ; 89: 203-14, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25675858

RESUMO

The purpose of this study was to distinguish and characterize university student typologies according to their life satisfaction and satisfaction with their food-related life. An online survey was applied between June and August 2013 in five state universities in Chile, to 369 university students (mean age = 20.9 years, SD = 2.27). The survey included the Health-related Quality of Life Index-4 (HRQOL), Satisfaction with Life Scale (SWLS), Satisfaction with Food-related Life Scale (SWFL), as well as questions about the place of residence, importance of food for well-being, frequency of meals in the place of residence and the frequency of consumption of eight food groups. A cluster analysis was used to determine student typologies. Three typologies of students were distinguished with significant differences in the average scores of the SWLS and SWFL scales, self-perception of health, days with mental health problems, number of days of health-related incapacity, place of residence, socioeconomic status, importance of food for well-being, frequency of breakfast and dinner in the place of residence, frequency of consumption of meat, milk, fruits and vegetables. It was found that most students with higher levels of life satisfaction and satisfaction with food-related life live with their parents, eat at home more frequently, report fewer health problems, have healthful eating habits and consider food very important for their well-being. Although it is necessary to promote or improve the campaigns that foster healthful eating in the entire university population, these campaigns must be specifically targeted to students who do not receive direct support from their families.


Assuntos
Dieta , Comportamento Alimentar , Saúde , Satisfação Pessoal , Qualidade de Vida , Estudantes , Universidades , Adulto , Atitude Frente a Saúde , Chile , Análise por Conglomerados , Autoavaliação Diagnóstica , Feminino , Humanos , Masculino , Refeições , Pais , Características de Residência , Classe Social , Inquéritos e Questionários , Adulto Jovem
15.
Span. j. psychol ; 18: e38.1-e38.14, 2015. tab
Artigo em Inglês | IBECS | ID: ibc-137227

RESUMO

This study aimed to assess the effect of satisfaction with food-related life on life satisfaction among inhabitants of the main municipalities of central Chile. A survey was applied to a sample of 1,277 people, distributed proportionally by municipality. The questionnaire included the following scales: SWLS (Satisfaction with Life Scale), SWFL (Satisfaction with Food-related Life) and the Health-Related Quality of Life Index (HRQOL). Questions were asked regarding eating habits inside and outside the home, time available for meals at home, the assessment of five sources of happiness and the demographic characteristics of those surveyed. An ordered logit model was proposed, in which the dependent variable was satisfaction with life. Satisfaction with life was significantly related to the respondent’s socioeconomic status, self-perception of health, degree of satisfaction with food-related life, monthly food expenditure, time available for supper with the family (p < .01); gender, self-reported number of days affected by mental health problems, frequency of supper with the family, the degree of agreement with respect to family being an important source of happiness (p < .05); and family size and frequency of food consumption in fast food outlets (p < .10). Satisfaction with life in the study sample is related to aspects associated with health, family and eating, and the family interaction associated with eating may play an important role in overall satisfaction with life (AU)


No disponible


Assuntos
Humanos , Adulto , Satisfação Pessoal , Alimentos , Família/psicologia , Qualidade de Vida/psicologia , Inquéritos e Questionários , Comportamento Alimentar/psicologia , Comportamento Alimentar/psicologia , Chile
16.
Nutr Hosp ; 30(2): 453-61, 2014 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-25208802

RESUMO

OBJECTIVE: To characterize university students typologies according to chronic food restriction, satisfaction with life and food consumption. MATERIALS AND METHOD: A questionnaire was applied on a non-probability sample of 369 male and female students from five Chilean universities. The questionnaire included: Revised Restraint Scale (RRS), Satisfaction with Life Scale (SWLS), Satisfaction with Food-related Life (SWFL) and the Health-related Quality of Life Index. The survey included food and drink consumption habits, weight and approximate height and sociodemographic variables. RESULTS: Two factors in the RRS were detected by exploratory factor analysis: Preoccupation with Diet (PD) and Weight fluctuations (WF). A confirmatory factor analysis validated the bifactor structure of the RRS with an acceptable adjustment kindness. The cluster analysis allowed a distinction of four typologies with a significant variation in PD, WF, SWLS and SWFL scoring, number of days with mental health problems, frequency of alcoholic drinks consumption, restraint on the consumption of certain foods, drinks and spices, consumption frequency of fruit out of the main meals and types. Typologies did not differ on their body mass index. CONCLUSIONS: Both, students preoccupied with diet and those who are not, experience higher levels of satisfaction with life and with food. Lower levels of global life satisfaction and satisfaction with food are related with the fluctuations in weight.


Assuntos
Dieta , Comportamento Alimentar , Chile , Feminino , Humanos , Masculino , Satisfação Pessoal , Qualidade de Vida , Estudantes , Inquéritos e Questionários , Universidades , Adulto Jovem
17.
J Bacteriol ; 196(21): 3746-55, 2014 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-25135218

RESUMO

Salmonella pathogenicity islands 1 and 2 (SPI-1 and SPI-2) have essential roles in the pathogenesis of Salmonella enterica. Previously, we reported transcriptional cross talk between SPI-1 and SPI-2 when the SPI-1 regulator HilD induces expression of the SsrA/B two-component system, the central positive regulator of SPI-2, during the growth of Salmonella to late stationary phase in LB rich medium. Here, we further define the mechanism of the HilD-mediated expression of ssrAB. Expression analysis of cat transcriptional fusions containing different regions of ssrAB revealed the presence of negative regulatory sequences located downstream of the ssrAB promoter. In the absence of these negative cis elements, ssrAB was expressed in a HilD-independent manner and was no longer repressed by the global regulator H-NS. Consistently, when the activity of H-NS was inactivated, the expression of ssrAB also became independent of HilD. Furthermore, electrophoretic mobility shift assays showed that both HilD and H-NS bind to the ssrAB region containing the repressing sequences. Moreover, HilD was able to displace H-NS bound to this region, whereas H-NS did not displace HilD. Our results support a model indicating that HilD displaces H-NS from a region downstream of the promoter of ssrAB by binding to sites overlapping or close to those sites bound by H-NS, which leads to the expression of ssrAB. Although the role of HilD as an antagonist of H-NS has been reported before for other genes, this is the first study showing that HilD is able to effectively displace H-NS from the promoter of one of its target genes.


Assuntos
Proteínas de Bactérias/metabolismo , Proteínas de Ligação a DNA/metabolismo , Regulação Bacteriana da Expressão Gênica/fisiologia , Proteínas de Membrana/metabolismo , Proteínas de Ligação a RNA/metabolismo , Salmonella enterica/metabolismo , Fatores de Transcrição/metabolismo , Proteínas de Bactérias/genética , Proteínas de Ligação a DNA/genética , Proteínas de Membrana/genética , Proteínas de Ligação a RNA/genética , Salmonella enterica/genética , Transcrição Genética
18.
Nutr. hosp ; 30(2): 453-461, ago. 2014. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-142546

RESUMO

Objective: To characterize university students typologies according to chronic food restriction, satisfaction with life and food consumption. Materials and method: A questionnaire was applied on a non-probability sample of 369 male and female students from five Chilean universities. The questionnaire included: Revised Restraint Scale (RRS), Satisfaction with Life Scale (SWLS), Satisfaction with Food-related Life (SWFL) and the Health-related Quality of Life Index. The survey included food and drink consumption habits, weight and approximate height and sociodemographic variables. Results: Two factors in the RRS were detected by exploratory factor analysis: Preoccupation with Diet (PD) and Weight fluctuations (WF). A confirmatory factor analysis validated the bifactor structure of the RRS with an acceptable adjustment kindness. The cluster analysis allowed a distinction of four typologies with a significant variation in PD, WF, SWLS and SWFL scoring, number of days with mental health problems, frequency of alcoholic drinks consumption, restraint on the consumption of certain foods, drinks and spices, consumption frequency of fruit out of the main meals and types. Typologies did not differ on their body mass index. Conclusions: Both, students preoccupied with diet and those who are not, experience higher levels of satisfaction with life and with food. Lower levels of global life satisfaction and satisfaction with food are related with the fluctuations in weight (AU)


Objetivo: Caracterizar tipologías de estudiantes universitarios según restricción alimentaria crónica, satisfacción con la vida y con la alimentación. Material y método: Se aplicó un cuestionario a una muestra no probabilística de 369 estudiantes de ambos géneros de cinco universidades de Chile. El cuestionario incluyó: Escala Revisada de Restricción Alimentaria (RRS), Satisfaction with Life Scale (SWLS), Satisfaction with Food-related Life (SWFL) y el Índice de Calidad de Vida relativo a la Salud. Se consultaron hábitos de consumo de alimentos y bebidas, peso y estatura aproximada y variables sociodemográficas. Resultados: Mediante análisis factorial exploratorio se detectaron dos factores en la RRS: Preocupación por la Dieta (PD) y Fluctuaciones de Peso (FP). Mediante análisis factorial confirmatorio se validó la estructura bifactorial de la RRS con una aceptable bondad de ajuste. Mediante análisis clúster se distinguieron cuatro tipologías que difirieron significativamente en los puntajes de PD y FP, los puntajes de la SWLS y SWFL, número de días con problemas de salud mental, frecuencia de consumo de bebidas alcohólicas, restricción del consumo de algunos alimentos, bebidas y condimentos, frecuencia de consumo de fruta a deshora y género. Las tipologías no difirieron en su índice de masa corporal. Conclusiones: Tanto los estudiantes que se preocupan por la dieta como los que no, experimentan mayores niveles satisfacción con la vida y con su alimentación. Menores niveles de satisfacción global y en el dominio de la alimentación se relacionan con las fluctuaciones de peso (AU)


Assuntos
Humanos , Adulto Jovem , Restrição Calórica/psicologia , Comportamento Alimentar , Comportamento Alimentar , Psicometria/instrumentação , Estudantes/estatística & dados numéricos , Satisfação Pessoal , Qualidade de Vida , Saúde Mental
19.
Microbiology ; 160(Pt 4): 733-41, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24440835

RESUMO

The Salmonella enterica ompS1 gene encodes a quiescent porin that belongs to the OmpC/OmpF family. In the present work we analysed the regulatory effects of OmpR phosphorylation on ompS1 expression. We found that in vivo, OmpR in its phosphorylated form (OmpR-P) was important in the regulation of the two ompS1 promoters: OmpR-P activated the P1 promoter and repressed the P2 promoter in an EnvZ-dependent manner; expression occurs from the P2 promoter in an ompR mutant. In vitro, OmpR-P had a higher DNA-binding-affinity to the ompS1 promoter region than OmpR and OmpRD55A, showing an affinity even higher than that of equivalent DNA regions in the 5'-upstream regulatory sequence of the major porin-encoding genes ompC and ompF. By analysing different environmental conditions, we found that glucose and glycerol enhanced ompS1 expression in the wild-type strain. Interestingly the stimulation by glycerol was OmpR-dependent while the effect of glucose was still observed in the absence of OmpR. Acetyl phosphate produced by the AckA-Pta pathway did not influence ompS1 regulation. These data indicate the important role of the phosphorylation in the activity of OmpR on the differential regulation of both ompS1 promoters and its impact on the pathogenesis.


Assuntos
Proteínas da Membrana Bacteriana Externa/biossíntese , Regulação Bacteriana da Expressão Gênica , Porinas/biossíntese , Regiões Promotoras Genéticas , Processamento de Proteína Pós-Traducional , Salmonella typhi/genética , Salmonella typhi/metabolismo , Fatores de Transcrição/metabolismo , Expressão Gênica/efeitos dos fármacos , Glucose/metabolismo , Glicerol/metabolismo , Fosforilação , Ligação Proteica
20.
Suma psicol ; 21(1): 54-62, ene.-jun. 2014. ilus, tab
Artigo em Espanhol | LILACS-Express | ID: lil-735191

RESUMO

El presente estudio tiene por objeto identificar variables que influyen en la satisfacción con la vida de personas pertenecientes a distintos niveles socioeconómicos (NSE) en el sur de Chile. Para lograr este objetivo, 316 residentes en las principales ciudades de esta parte del país, distribuidos proporcionalmente por comuna, respondieron un cuestionario que incluía las escalas SWLS (Satisfacción con la vida) y SWFL (Satisfacción con la alimentación), el Índice de Calidad de Vida Relativo a la Salud (ICVRS) y la Escala de Discrepancia del Yo. Además se consultaron las características demográficas de los encuestados. Como resultado se obtuvo que la mayor proporción de personas extremadamente satisfechas con su vida y su alimentación se encuentran en el NSE más alto.Asimismo, se plantearon cuatro modelos logit ordinales, en los cuales la variable dependiente fue la satisfacción con la vida, considerando la muestra total y tres submuestras: estratos alto y medio-alto, medio-medio y en conjunto los niveles medio-bajo, bajo y muy bajo. Estos cuatro modelos resultaron significativos, pero difirieron en las variables explicativas que fueron significativas. También se evidenció que, independientemente del NSE, las personas con alta satisfacción con su alimentación tienen mayor probabilidad de tener alta satisfacción con la vida. Por último, se obtuvo que otras variables que se relacionan con la satisfacción con la vida son la salud, la familia, la discrepancia con el yo ideal, la edad y la tenencia de bienes en el hogar.


This study aimed to identify the variables influencing satisfaction with life in people from different socioeconomic groups in southern Chile.To do this, a questionnaire was applied to a sample of 316 residents in the main cities of the southern side of the country, distributed Ordinal logit Socioeconomic level proportionally by municipality. The questionnaire included the Satisfaction with Life Scale (SWLS), Satisfaction with Food-related Life (SWFL), the Health-Related Quality of Life Index (HRQOL), the Self-Discrepancy Scale, and questions on demographic characteristics of those surveyed. The highest proportion of people extremely satisfied with their life and food-related life was found in the highest socioeconomic group. Four multinomial logit models were considered, in which the dependent variable was satisfaction with life. This analysis comprised the total sample and three sub-samples: high and upper-middle class, middle-middle class, and lower-middle, low and very-low classes considered jointly. The four models were significant, but differed in the explanatory variables that were significant. Regardless of socioeconomic status, individuals who experienced high satisfaction with their food-related life were more likely to report high life satisfaction. Other variables related to satisfaction with life were health, family, discrepancy with the ideal self, age and ownership of certain goods in the home.

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