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2.
Vet Parasitol ; 271: 51-53, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31303203

RESUMO

The consumption of undercooked infected chicken can be a source of infection for humans and carnivores regarding the zoonotic protozoan parasite Toxoplasma gondii. Furthermore, free-range chickens are sentinels for the presence of T. gondii oocysts in the environment because they feed from the ground. By using the modified agglutination test (MAT), we investigated the presence of antibodies to T. gondii in 178 free-range and 170 broiler chickens raised indoors and slaughtered in Portugal. Prevalence of specific antibodies was 5.6% in free-range and 0.0% in broiler chickens raised indoors (p =  0.002).


Assuntos
Anticorpos Antiprotozoários/sangue , Galinhas , Toxoplasma/imunologia , Toxoplasmose Animal/sangue , Toxoplasmose Animal/epidemiologia , Criação de Animais Domésticos , Animais , Estudos Soroepidemiológicos
3.
J Clin Immunol ; 39(5): 494-504, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31172380

RESUMO

PURPOSE: Colitis is a common and serious complication of chronic granulomatous disorder (CGD) and requires assessment. Colonoscopy is invasive and carries risks of serious complication. We therefore assessed non-invasive monitoring via magnetic resonance imaging (MRI). We also evaluated fecal calprotectin (FCP), the Harvey-Bradshaw index (HBI) clinical score, and serum cytokines. METHODS: We recruited 10 patients with CGD (8 males, mean age 29.6 years), scored a modified HBI, and obtained stool for FCP. The following day we took blood for cytokine measurement via Luminex, performed MR enterography (scored by two independent radiologists using three systems: London score, CDMI, and MaRIA) followed by colonoscopy with disease activity measurement via ulcerative colitis endoscopic index of severity (UCEIS). We assessed patient experience after each investigation and overall preference with follow-up questionnaires. RESULTS: MRI scores correlated well with colonoscopic gold standard (for London score R2 0.91, p < 0.0001; for CDMI R2 0.83, p = 0.0006; for MaRIA R2 0.89, p = 0.0002). MRI was better tolerated and generally preferred, quicker, and visualized the entire large bowel whereas colonoscopy did not reach the terminal ileum in 3 participants. Elevated FCP accurately differentiated patients with colitis from those without, and log(calprotectin) correlated well with disease activity (R2 0.71, p = 0.009). Serum interleukin (IL)-12 concentration correlated with colitis activity but IL-1ß and TNF did not. Harvey-Bradshaw index did not correlate with colitis activity. CONCLUSIONS: MRI and fecal calprotectin are useful methods for monitoring CGD colitis and should reduce the need for colonoscopy in these patients. IL-12 may represent an appropriate target for treatment.

4.
Ortho Sci., Orthod. sci. pract ; 12(47): 145-151, 2019. tab, ilus
Artigo em Inglês | BBO - Odontologia | ID: biblio-1023059

RESUMO

Os sistemas CAD/CAM, incluindo o sistema eXceed™, possibilitam aos ortodontistas a realização de diagnóstico, planejamento de casos clínicos e posicionamento virtual de bráquetes. A posição de cada acessório, determinada pelo software, é transferida para o modelo tridimensional do paciente sobre o qual um dispositivo de transferência para colagem indireta (CI) de bráquetes é produzido. A CI permite uma melhoria na precisão da colagem de acessórios ortodônticos, diminuindo o tempo e o custo do tratamento devido a uma menor necessidade de dobras de finalização e reposicionamento de bráquetes. O caso clínico apresentado tem o objetivo de apresentar um tratamento ortodôntico utilizando o método de CI com posicionamento virtual de bráquetes idealizado pelo sistema eXceed™. O posicionamento dos bráquetes programado pelo sistema eXceed™ permitiu um posicionamento "ideal" dos dentes dispensando a implementação de dobras de finalização e proporcionando uma oclusão equilibrada. Essa nova tecnologia demonstrou ser eficiente e eficaz, e o setup ortodôntico fornecido pelo sistema ficou acordante com o resultado final do tratamento ortodôntico (AU)


CAD/CAM systems, including the eXceed™ system, allow orthodontists to perform diagnosis, treatment plan, and brackets virtual positioning. The positioning of each bracket, determined by the software, is transferred to a tridimensional patient model enabling to build an indirect bonding (IB) tray. IB allows improvement in the ideal bracket positioning, decreasing treatment time and cost due to less detailed finishing bends and/or bracket repositioning. The clinical case report purpose is to present an orthodontic treatment using IB with virtual bracket positioning using eXceed™ system. The bracket virtual positioning from eXceed™ system allowed an ideal final teeth positioning eliminating detailed finishing bends and reaching the ideal occlusion as planned. This new technology has shown to be efficient and effective, and the virtual tridimensional orthodontic setup has been in agreement with the final treatment occlusion (AU)


Assuntos
Humanos , Masculino , Adulto , Aparelhos Ortodônticos , Ortodontia Corretiva , Projeto Auxiliado por Computador
5.
J Anal Toxicol ; 43(4): 307-315, 2019 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-30561666

RESUMO

The development of accurate and reliable analytical methodologies to detect the abuse of doping agents in sport animals is crucial to ensure their welfare, as well as to support continuing social acceptance of these sports. The detection of doping agents in racing pigeons is difficult, especially owing to the disadvantages and limitations of obtaining samples from conventional matrices. The present study aimed to develop and validate an analytical methodology combining a two-step extraction procedure (liquid-liquid extraction and solid-phase extraction) in feathers from racing pigeons with analysis by liquid chromatography tandem mass spectrometry (LC-MS-MS) that enabled the simultaneous detection of a beta-agonist drug (clenbuterol) and three corticosteroids (prednisolone, betamethasone and budesonide). The method was validated concerning linearity (with coefficients of determination always higher than 0.99), accuracy (87.3-112.4%), precision (repeatability and intermediate precision coefficient of variation (CV%) always below 15%), recovery (71.6-98.2%), limits of detection (0.24-0.52 ng/g) and quantification (0.79 and 0. 1.74 ng/g) and specificity. The applicability of the method was performed using feathers from pigeons administered orally with a daily dose of 0.075 mg of betamethasone. The drug was administered during 60 days and successive analyses of feathers were performed, at the end of the administration protocol and also after ceasing the oral administration of the drug, for a three weeks period.


Assuntos
Corticosteroides/análise , Betametasona/análise , Columbidae/fisiologia , Doping nos Esportes/prevenção & controle , Plumas/química , Detecção do Abuso de Substâncias/métodos , Administração Oral , Corticosteroides/administração & dosagem , Agonistas Adrenérgicos beta/análise , Animais , Betametasona/administração & dosagem , Cromatografia Líquida , Clembuterol/análise , Confiabilidade dos Dados , Inspeção de Alimentos/métodos , Limite de Detecção , Sensibilidade e Especificidade , Espectrometria de Massas em Tandem
6.
PLoS Negl Trop Dis ; 12(11): e0006880, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30418969

RESUMO

The recent emergence of Zika virus (ZIKV) in the Americas coincident with increased caseloads of microcephalic infants and Guillain-Barre syndrome has prompted a flurry of research on ZIKV. Much of the research is difficult to compare or repeat because individual laboratories use different virus isolates, growth conditions, and quantitative assays. Here we obtained three readily available contemporary ZIKV isolates and the prototype Ugandan isolate. We generated stocks of each on Vero mammalian cells (ZIKVmam) and C6/36 mosquito cells (ZIKVmos), determined titers by different assays side-by-side, compared growth characteristics using one-step and multi-step growth curves on Vero and C6/36 cells, and examined plaque phenotype. ZIKV titers consistently peaked earlier on Vero cells than on C6/36 cells. Contemporary ZIKV isolates reached peak titer most quickly in a multi-step growth curve when the amplifying cell line was the same as the titering cell line (e.g., ZIKVmam titered on Vero cells). Growth of ZIKVmam on mosquito cells was particularly delayed. These data suggest that the ability to infect and/or replicate in insect cells is limited after growth in mammalian cells. In addition, ZIKVmos typically had smaller, more homogenous plaques than ZIKVmam in a standard plaque assay. We hypothesized that the plaque size difference represented early adaptation to growth in mammalian cells. We plaque purified representative-sized plaques from ZIKVmos and ZIKVmam. ZIKVmos isolates maintained the initial phenotype while plaques from ZIKVmam isolates became larger with passaging. Our results underscore the importance of the cells used to produce viral stocks and the potential for adaptation with minimal cell passages. In addition, these studies provide a foundation to compare current and emerging ZIKV isolates in vitro and in vivo.

7.
J Tissue Viability ; 27(4): 249-256, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30318397

RESUMO

Burns are injuries caused mainly by thermal trauma, which can progress to unsatisfactory results healing. This study aimed to evaluate the biomaterial (bacterial cellulose membrane) and photobiomodulation, exclusively and associated, in the treatment of third degree burns in rats. Forty male Wistar rats (±280 g) were randomly divided into four groups, with 10 animals each: control group (CG); bacterial cellulose membrane group (MG); laser group (LG) and bacterial cellulose membrane and laser group (MG + L). The burn was caused with a 1 cm2 aluminum plate heated to 150 °C and pressed on the animal's back for 10 s. The treatments were started immediately after induction of injury. For to laser irradiation (660 nm, 100 mW, 25 J/cm2 and energy of 1 J) on five distinct application points were used, on alternate days, a total of five sessions. After ten days of treatment the animals were euthanized for collected samples. One-way ANOVA and Tukey's tests (P < 0.05) were used. Histological analysis revealed differences regarding the healing process phase in each experimental group. MG showed the proliferative phase. The LG demonstrated greater amount of blood vessels and immune expression of VEGF. However, when the treatments were combined, the number of vessels and the immune expression of VEGF factor was lower than LG. Thus, it was concluded that both treatments proposed (biomaterial and LLLT) are good alternatives for third degree burns when applied isolated because they stimulate the healing process by acting on the modulation of the inflammatory phase and promote stimulation of angiogenesis.

8.
J Infect Dis ; 2018 Aug 18.
Artigo em Inglês | MEDLINE | ID: mdl-30137432

RESUMO

Background: Persistent hepatitis E virus (HEV) infection is described in a number of immunosuppressive conditions. We aimed to determine the risk of persistent HEV infection in patients with primary or secondary antibody deficiency. Methods: Two hundred and forty five antibody deficient patients on regular immunoglobulin replacement were tested for HEV RNA and anti-HEV IgG. Immunoglobulin products and plasma from nine antibody-deficient patients pre- and post-IVIG, five recently treated patients with persistent HEV infection and five healthy patients recovered from acute HEV infection were analysed for anti-HEV IgG and for antibody reacting with HEV antigen (HEV-Ag). Results: No antibody deficient patient had detectable plasma HEV RNA. Anti-HEV IgG was detected in 38.8% of patients. All ten immunoglobulin products tested contained anti-HEV capable of neutralising HEV-Ag. Plasma samples following IVIG infusion demonstrated higher anti-HEV IgG and neutralising activity compared with pre-IVIG samples. Neutralising activity was similar to healthy patients with recent acute HEV infection. Conclusion: The risk of persistent HEV infection in patients with antibody deficiency appears extremely low. This may be due to passive seroprotection afforded by the ubiquitous presence of anti-HEV in immunoglobulin replacement products.

9.
Artigo em Inglês | MEDLINE | ID: mdl-29736679

RESUMO

The present study attempts to better identify the neurophysiological changes occurring during flow experience and how this can be related to the mobilization of attentional resources. Self-reports of flow (using a flow feelings scale) and attention (using thought probes), autonomic activity (heart rate, heart rate variability, and breathing rate), and cerebral oxygenation (using near-infrared spectroscopy) in two regions of the frontoparietal attention network (right lateral frontal cortex and right inferior parietal lobe) were measured during the practice of two simple video games (Tetris and Pong) played at different difficulty conditions (easy, optimal, hard, or self-selected). Our results indicated that an optimal level of difficulty, compared with an easy or hard level of difficulty led to greater flow feelings and a higher concentration of oxygenated hemoglobin in the regions of the frontoparietal network. The self-selected, named autonomy condition did not lead to more flow feelings than the optimal condition; however, the autonomy condition led to greater sympathetic activity (reduced heart rate variability and greater breathing rate) and higher activation of the frontoparietal regions. Our study suggests that flow feelings are highly connected to the mobilization of attentional resources, and all the more in a condition that promotes individuals' choice and autonomy.

10.
Artigo em Inglês | MEDLINE | ID: mdl-29753212

RESUMO

The use of performance enhancing drugs is not only common in humans, but also in animal sports, including racing of horses, greyhounds and pigeons. The development of accurate analytical procedures to detect doping agents in sports is crucial in order to protect the fair-play of the game, avoid financial fraud in the attribution of eventual awards and, even more important, to protect the animals from harmful drugs and/or dangerous dosage regimens. The present study aimed to develop and validate, a method that enabled the screening and confirmation of the presence of a beta-agonist (clenbuterol) and three corticosteroids (betamethasone, prednisolone and budesonide) in faeces from pigeons. The extraction procedure entailed the combination of liquid-liquid extraction with solid-phase extraction and the analysis was performed by liquid- chromatography coupled to tandem mass spectrometry, with a single 15 minute chromatographic run-time. The method was validated concerning selectivity, linearity (with coefficients of determination always >0.99), accuracy (87.5-114.9%), inter-day and intra-day precisions, limits of detection (0.14-1.81 ng/g) and limits of quantification (0.49-6.08 ng/g), stability and extraction recovery (71.0%-99.3%). The method was successfully applied for the analysis of samples from two pigeons that had been orally administered betamethasone, demonstrating its suitability for doping control purposes.


Assuntos
Columbidae/metabolismo , Fezes/química , Detecção do Abuso de Substâncias/métodos , Espectrometria de Massas em Tandem/métodos , Corticosteroides/análise , Corticosteroides/metabolismo , Animais , Calibragem , Fracionamento Químico/métodos , Cromatografia Líquida de Alta Pressão/métodos , Clembuterol/análise , Clembuterol/metabolismo , Doping nos Esportes/métodos , Limite de Detecção , Reprodutibilidade dos Testes
11.
Insect Biochem Mol Biol ; 95: 1-9, 2018 04.
Artigo em Inglês | MEDLINE | ID: mdl-29526772

RESUMO

Previously, a Sanger-based sialotranscriptome analysis of adult female Culex tarsalis was published based on ∼2000 ESTs. During the elapsed 7.5 years, pyrosequencing has been discontinued and Illumina sequences have increased considerable in size and decreased in price. We here report an Illumina-based sialotranscriptome that allowed finding the missing apyrase from the salivary transcriptome of C. tarsalis, to determine several full-length members of the 34-62 kDa family, when a single EST has been found previously, in addition to identifying many salivary families with lower expression levels that were not detected previously. The use of multiple libraries including salivary glands and carcasses from male and female organisms allowed for an unprecedented insight into the tissue specificity of transcripts, and in this particular case permitting identification of transcripts putatively associated with blood feeding, when exclusive of female salivary glands, or associated with sugar feeding, when transcripts are found upregulated in both male and female glands.


Assuntos
Culex/metabolismo , Proteínas de Insetos/biossíntese , Caracteres Sexuais , Sialoglicoproteínas/biossíntese , Animais , Feminino , Perfilação da Expressão Gênica , Masculino
12.
Front Immunol ; 9: 543, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29599784

RESUMO

Activated phosphoinositide 3-kinase (PI3K) δ Syndrome (APDS), caused by autosomal dominant mutations in PIK3CD (APDS1) or PIK3R1 (APDS2), is a heterogeneous primary immunodeficiency. While initial cohort-descriptions summarized the spectrum of clinical and immunological manifestations, questions about long-term disease evolution and response to therapy remain. The prospective European Society for Immunodeficiencies (ESID)-APDS registry aims to characterize the disease course, identify outcome predictors, and evaluate treatment responses. So far, 77 patients have been recruited (51 APDS1, 26 APDS2). Analysis of disease evolution in the first 68 patients pinpoints the early occurrence of recurrent respiratory infections followed by chronic lymphoproliferation, gastrointestinal manifestations, and cytopenias. Although most manifestations occur by age 15, adult-onset and asymptomatic courses were documented. Bronchiectasis was observed in 24/40 APDS1 patients who received a CT-scan compared with 4/15 APDS2 patients. By age 20, half of the patients had received at least one immunosuppressant, but 2-3 lines of immunosuppressive therapy were not unusual before age 10. Response to rapamycin was rated by physician visual analog scale as good in 10, moderate in 9, and poor in 7. Lymphoproliferation showed the best response (8 complete, 11 partial, 6 no remission), while bowel inflammation (3 complete, 3 partial, 9 no remission) and cytopenia (3 complete, 2 partial, 9 no remission) responded less well. Hence, non-lymphoproliferative manifestations should be a key target for novel therapies. This report from the ESID-APDS registry provides comprehensive baseline documentation for a growing cohort that will be followed prospectively to establish prognostic factors and identify patients for treatment studies.

14.
Biomed Mater Eng ; 29(1): 29-42, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29254071

RESUMO

INTRODUCTION: Burn injuries represent a high risk of morbidity and mortality. The wound healing process is complex and requires the participation of different types of cells. Therefore, new biomaterials, which innovate the wound healing process, are being investigated. OBJECTIVE: The aim of this study was to investigate the use of bacterial cellulose both in its pure state and enriched with lidocaine in full-thickness burns in rats. METHODS: Thirty rats (Wistar) (260 ± 20 gramas) divided into control group (CG), bacterial cellulose membrane group (MG) and bacterial cellulose membrane enriched with lidocaine group (MLG) were used. The burns were induced using a 150°C heated soldering iron, held on the animal neck for 10 seconds. The biomaterial was applied immediately after injury and skin samples were collected on the tenth day of the treatment. The level of significance of p⩽0.05 was used for the conclusion of the statistical analysis. RESULTS: The groups treated with the biomaterials, a histological pattern compatible with a more advanced repair stage showing skin appendages, mild inflammatory infiltrate, better collagen fiber organization and mild immunostaining COX-2 and MMP-9 was observed, when compared to the control group that did not receive any type of treatment. CONCLUSION: Thus, was concluded that the bacterial cellulose-based biomaterial both in its pure state and enriched with lidocaine optimizing the full-thickness burn wound healing in rats.


Assuntos
Materiais Biocompatíveis/uso terapêutico , Curativos Biológicos , Queimaduras/terapia , Celulose/uso terapêutico , Polissacarídeos Bacterianos/uso terapêutico , Anestésicos Locais/uso terapêutico , Animais , Materiais Biocompatíveis/química , Queimaduras/patologia , Celulose/química , Lidocaína/uso terapêutico , Masculino , Membranas Artificiais , Polissacarídeos Bacterianos/química , Ratos Wistar , Pele/efeitos dos fármacos , Pele/patologia , Cicatrização/efeitos dos fármacos
15.
Clin Biomech (Bristol, Avon) ; 44: 83-89, 2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-28364674

RESUMO

BACKGROUND: There is a frequent complaint about balance problems among fibromyalgia syndrome patients; however, there are not enough studies that have shown static postural sway of women with fibromyalgia syndrome. This study aimed to compare static postural sway of women with and without fibromyalgia syndrome. METHODS: This is a cross-sectional study in which twenty-nine women with fibromyalgia syndrome and 20 without took part. A posturography evaluation was performed in six different situations (bipedal, right tandem and left tandem, with eyes opened and closed), and questionnaires for clinical depression symptoms, clinical anxiety symptoms, sleep quality, and Visual Analogue Scales for Pain and Fatigue were applied. Mann-Whitney U test was used to check differences among groups; Wilcoxon matched-pair test was used to check differences intragroup; Cohen d coefficient was used to measure effect sizes and Pearson Correlation Coefficient was used for correlations among variables. Level of significance adopted was 5%. FINDINGS: Women with fibromyalgia syndrome have presented worse postural sway than women without fibromyalgia syndrome in all situations (P<0.05), and worse scores in all questionnaires (P<0.05). In the eyes closed situations, women with fibromyalgia syndrome presented worse postural sway than women without in the same conditions. INTERPRETATION: Women with fibromyalgia syndrome have worse performance in the static posture test, more prominent in reduced support bases with eyes closed. Pain, fatigue, depression and anxiety may have directly influenced postural sway in fibromyalgia syndrome patients.


Assuntos
Fibromialgia/fisiopatologia , Equilíbrio Postural/fisiologia , Adulto , Estudos Transversais , Fadiga/fisiopatologia , Feminino , Humanos , Pessoa de Meia-Idade , Dor/fisiopatologia , Medição da Dor , Inquéritos e Questionários
16.
PLoS Negl Trop Dis ; 11(2): e0005394, 2017 02.
Artigo em Inglês | MEDLINE | ID: mdl-28187142

RESUMO

Half of the human population is at risk of infection by an arthropod-borne virus. Many of these arboviruses, such as West Nile, dengue, and Zika viruses, infect humans by way of a bite from an infected mosquito. This infectious inoculum is insect cell-derived giving the virus particles distinct qualities not present in secondary infectious virus particles produced by infected vertebrate host cells. The insect cell-derived particles differ in the glycosylation of virus structural proteins and the lipid content of the envelope, as well as their induction of cytokines. Thus, in order to accurately mimic the inoculum delivered by arthropods, arboviruses should be derived from arthropod cells. Previous studies have packaged replicon genome in mammalian cells to produce replicon particles, which undergo only one round of infection, but no studies exist packaging replicon particles in mosquito cells. Here we optimized the packaging of West Nile virus replicon genome in mosquito cells and produced replicon particles at high concentration, allowing us to mimic mosquito cell-derived viral inoculum. These particles were mature with similar genome equivalents-to-infectious units as full-length West Nile virus. We then compared the mosquito cell-derived particles to mammalian cell-derived particles in mice. Both replicon particles infected skin at the inoculation site and the draining lymph node by 3 hours post-inoculation. The mammalian cell-derived replicon particles spread from the site of inoculation to the spleen and contralateral lymph nodes significantly more than the particles derived from mosquito cells. This in vivo difference in spread of West Nile replicons in the inoculum demonstrates the importance of using arthropod cell-derived particles to model early events in arboviral infection and highlights the value of these novel arthropod cell-derived replicon particles for studying the earliest virus-host interactions for arboviruses.


Assuntos
Aedes/virologia , Infecções por Arbovirus/virologia , Arbovirus/fisiologia , Insetos Vetores/virologia , Replicon , Febre do Nilo Ocidental/virologia , Vírus do Nilo Ocidental/genética , Animais , Arbovirus/genética , Feminino , Humanos , Camundongos , Camundongos Endogâmicos C57BL , Proteínas Estruturais Virais/genética , Proteínas Estruturais Virais/metabolismo , Montagem de Vírus , Cultura de Vírus , Vírus do Nilo Ocidental/crescimento & desenvolvimento , Vírus do Nilo Ocidental/fisiologia
17.
Microsc Res Tech ; 79(4): 313-20, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26853699

RESUMO

Burns are injuries caused by direct or indirect contact to chemical, physical, or biological agents. Low-level laser therapy (LLLT) is a promising treatment since it is low-cost, non-invasive, and induces cell proliferation. This study aimed to investigate the effects of LLLT (660 nm) at two different fluences (12.5 J/cm(2) and 25 J/cm(2) ) per point of application on third-degree burns in rats. Thirty rats (Wistar) divided into GC, GL12.5, and GL25 were used in the study, and submitted to burn injury through a soldering iron at 150°C, pressed on their back for 10 s. LLLT was applied immediately, and 2, 4, 6, and 8 days after wound induction. Histological analysis revealed a decreased inflammatory infiltrate in the group treated with 25 J/cm(2) , and intense inflammatory infiltrate in the control group and in the group treated with 12.5 J/cm(2) . The immunostaining of COX-2 was more intense in the control groups and in the group treated with 12.5 J/cm(2) than in the group treated with 25 J/cm(2) . Conversely, VEGF immunomarking was more expressive in the group treated with 25 J/cm(2) than it was in the other two groups. Therefore, our findings suggest that the use of 25 J/cm(2) and 1 J of energy was more effective in stimulating the cellular processes involved in tissue repair on third-degree burns in rats by reducing the inflammatory phase, and stimulating angiogenesis, thus restoring the local microcirculation which is essential for cell migration.


Assuntos
Queimaduras/terapia , Terapia com Luz de Baixa Intensidade/métodos , Animais , Queimaduras/patologia , Queimaduras/fisiopatologia , Modelos Animais de Doenças , Humanos , Terapia com Luz de Baixa Intensidade/instrumentação , Masculino , Ratos , Ratos Wistar , Cicatrização/efeitos da radiação
18.
In. Santos, Elizabete Silva dos; Trindade, Pedro Henrique Duccini Mendes; Moreira, Humberto Graner. Tratado Dante Pazzanese de emergências cardiovasculares. São Paulo, Atheneu, 2016. p.921-933, ilus, tab.
Monografia em Português | Sec. Est. Saúde SP, SESSP-IDPCPROD, Sec. Est. Saúde SP | ID: ses-33170
19.
Sci Rep ; 5: 14761, 2015 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-26434597

RESUMO

The prospect of carbon-based magnetic materials is of immense fundamental and practical importance, and information on atomic-scale features is required for a better understanding of the mechanisms leading to carbon magnetism. Here we report the first direct detection of the microscopic magnetic field produced at (13)C nuclei in a ferromagnetic carbon material by zero-field nuclear magnetic resonance (NMR). Electronic structure calculations carried out in nanosized model systems with different classes of structural defects show a similar range of magnetic field values (18-21 T) for all investigated systems, in agreement with the NMR experiments. Our results are strong evidence of the intrinsic nature of defect-induced magnetism in magnetic carbons and establish the magnitude of the hyperfine magnetic field created in the neighbourhood of the defects that lead to magnetic order in these materials.

20.
Vet Ital ; 51(2): 93-8, 2015 Apr-Jun.
Artigo em Inglês | MEDLINE | ID: mdl-26129659

RESUMO

Since modernization and expansion of the poultry industry, infections with Mycoplasma spp. bacteria have been reported as a cause of considerable economic losses. The prevalence of Mycoplasma synoviae infection in 974,000 Portuguese broiler breeders, belonging to 36 flocks, was investigated from December 2008 to March 2012. This study was conducted using a commercial indirect enzyme-linked immunosorbent assay (ELISA) for the analysis of serum antibodies, and a polymerase chain reaction (PCR) for the tracheal tissue. Twenty-four flocks were simultaneously found positive by ELISA and PCR [66.7%, 95% confidence interval (CI): 43.5-76.9%]. The M. synoviae prevalence among chickens averaged 40.3% (483/1,200), with values ranging from 0.0 to 83.3% per flock. The prevalence of farms where M. synoviae positive birds have been found was determined in different poultry categories such as density, biosecurity, strains, offspring quality, premises'age, and others husbandry factors. Prevalence values were significantly higher among birds housed in new facilities (less than 3 years old) and were also significantly higher in the production period. The high prevalence of M. synoviae infection detected in the present study suggests the need to adopt appropriate control measures.


Assuntos
Criação de Animais Domésticos , Galinhas , Infecções por Mycoplasma/veterinária , Mycoplasma synoviae , Doenças das Aves Domésticas/epidemiologia , Animais , Estudos Epidemiológicos , Infecções por Mycoplasma/sangue , Infecções por Mycoplasma/epidemiologia , Reação em Cadeia da Polimerase , Portugal , Doenças das Aves Domésticas/sangue , Prevalência
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