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2.
Vet Parasitol ; 271: 51-53, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31303203

RESUMO

The consumption of undercooked infected chicken can be a source of infection for humans and carnivores regarding the zoonotic protozoan parasite Toxoplasma gondii. Furthermore, free-range chickens are sentinels for the presence of T. gondii oocysts in the environment because they feed from the ground. By using the modified agglutination test (MAT), we investigated the presence of antibodies to T. gondii in 178 free-range and 170 broiler chickens raised indoors and slaughtered in Portugal. Prevalence of specific antibodies was 5.6% in free-range and 0.0% in broiler chickens raised indoors (p =  0.002).


Assuntos
Anticorpos Antiprotozoários/sangue , Galinhas , Toxoplasma/imunologia , Toxoplasmose Animal/sangue , Toxoplasmose Animal/epidemiologia , Criação de Animais Domésticos , Animais , Estudos Soroepidemiológicos
3.
J Clin Immunol ; 39(5): 494-504, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31172380

RESUMO

PURPOSE: Colitis is a common and serious complication of chronic granulomatous disorder (CGD) and requires assessment. Colonoscopy is invasive and carries risks of serious complication. We therefore assessed non-invasive monitoring via magnetic resonance imaging (MRI). We also evaluated fecal calprotectin (FCP), the Harvey-Bradshaw index (HBI) clinical score, and serum cytokines. METHODS: We recruited 10 patients with CGD (8 males, mean age 29.6 years), scored a modified HBI, and obtained stool for FCP. The following day we took blood for cytokine measurement via Luminex, performed MR enterography (scored by two independent radiologists using three systems: London score, CDMI, and MaRIA) followed by colonoscopy with disease activity measurement via ulcerative colitis endoscopic index of severity (UCEIS). We assessed patient experience after each investigation and overall preference with follow-up questionnaires. RESULTS: MRI scores correlated well with colonoscopic gold standard (for London score R2 0.91, p < 0.0001; for CDMI R2 0.83, p = 0.0006; for MaRIA R2 0.89, p = 0.0002). MRI was better tolerated and generally preferred, quicker, and visualized the entire large bowel whereas colonoscopy did not reach the terminal ileum in 3 participants. Elevated FCP accurately differentiated patients with colitis from those without, and log(calprotectin) correlated well with disease activity (R2 0.71, p = 0.009). Serum interleukin (IL)-12 concentration correlated with colitis activity but IL-1ß and TNF did not. Harvey-Bradshaw index did not correlate with colitis activity. CONCLUSIONS: MRI and fecal calprotectin are useful methods for monitoring CGD colitis and should reduce the need for colonoscopy in these patients. IL-12 may represent an appropriate target for treatment.

4.
J Infect Dis ; 219(2): 245-253, 2019 01 07.
Artigo em Inglês | MEDLINE | ID: mdl-30137432

RESUMO

Background: Persistent hepatitis E virus (HEV) infection is described in a number of immunosuppressive conditions. We aimed to determine the risk of persistent HEV infection in patients with primary or secondary antibody deficiency. Methods: Two hundred forty-five antibody-deficient patients receiving regular immunoglobulin replacement therapy were tested for HEV RNA and anti-HEV immunoglobulin G (IgG). Immunoglobulin products and plasma specimens obtained from 9 antibody-deficient patients before and after intravenous immunoglobulin (IVIG) therapy, 5 recently treated patients with persistent HEV infection, and 5 healthy patients recovered from acute HEV infection were analyzed for anti-HEV IgG and for antibody reacting with HEV antigen. Results: No antibody-deficient patient had detectable plasma HEV RNA. Anti-HEV IgG was detected in 38.8% of patients. All 10 immunoglobulin products tested contained anti-HEV capable of neutralizing HEV antigen. Plasma samples collected following IVIG infusion therapy demonstrated a higher anti-HEV IgG level and neutralizing activity, compared with samples collected before IVIG therapy. Neutralizing activity was similar to that in healthy patients with recent acute HEV infection. Conclusion: The risk of persistent HEV infection in patients with antibody deficiency appears extremely low. This may be due to passive seroprotection afforded by the ubiquitous presence of anti-HEV in immunoglobulin replacement products.


Assuntos
Anticorpos Neutralizantes/imunologia , Anticorpos Anti-Hepatite/uso terapêutico , Hepatite E/imunologia , Hepatite E/terapia , Imunoglobulinas Intravenosas/uso terapêutico , Síndromes de Imunodeficiência/imunologia , Adulto , Idoso , Estudos Transversais , Feminino , Anticorpos Anti-Hepatite/sangue , Vírus da Hepatite E , Humanos , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Imunoglobulinas Intravenosas/sangue , Síndromes de Imunodeficiência/complicações , Masculino , Pessoa de Meia-Idade , RNA Viral/sangue
5.
J Anal Toxicol ; 43(4): 307-315, 2019 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-30561666

RESUMO

The development of accurate and reliable analytical methodologies to detect the abuse of doping agents in sport animals is crucial to ensure their welfare, as well as to support continuing social acceptance of these sports. The detection of doping agents in racing pigeons is difficult, especially owing to the disadvantages and limitations of obtaining samples from conventional matrices. The present study aimed to develop and validate an analytical methodology combining a two-step extraction procedure (liquid-liquid extraction and solid-phase extraction) in feathers from racing pigeons with analysis by liquid chromatography tandem mass spectrometry (LC-MS-MS) that enabled the simultaneous detection of a beta-agonist drug (clenbuterol) and three corticosteroids (prednisolone, betamethasone and budesonide). The method was validated concerning linearity (with coefficients of determination always higher than 0.99), accuracy (87.3-112.4%), precision (repeatability and intermediate precision coefficient of variation (CV%) always below 15%), recovery (71.6-98.2%), limits of detection (0.24-0.52 ng/g) and quantification (0.79 and 0. 1.74 ng/g) and specificity. The applicability of the method was performed using feathers from pigeons administered orally with a daily dose of 0.075 mg of betamethasone. The drug was administered during 60 days and successive analyses of feathers were performed, at the end of the administration protocol and also after ceasing the oral administration of the drug, for a three weeks period.


Assuntos
Corticosteroides/análise , Betametasona/análise , Columbidae/fisiologia , Doping nos Esportes/prevenção & controle , Plumas/química , Detecção do Abuso de Substâncias/métodos , Administração Oral , Corticosteroides/administração & dosagem , Agonistas Adrenérgicos beta/análise , Animais , Betametasona/administração & dosagem , Cromatografia Líquida , Clembuterol/análise , Confiabilidade dos Dados , Inspeção de Alimentos/métodos , Limite de Detecção , Sensibilidade e Especificidade , Espectrometria de Massas em Tandem
6.
Ortho Sci., Orthod. sci. pract ; 12(47): 145-151, 2019. tab, ilus
Artigo em Inglês | BBO - Odontologia | ID: biblio-1023059

RESUMO

Os sistemas CAD/CAM, incluindo o sistema eXceed™, possibilitam aos ortodontistas a realização de diagnóstico, planejamento de casos clínicos e posicionamento virtual de bráquetes. A posição de cada acessório, determinada pelo software, é transferida para o modelo tridimensional do paciente sobre o qual um dispositivo de transferência para colagem indireta (CI) de bráquetes é produzido. A CI permite uma melhoria na precisão da colagem de acessórios ortodônticos, diminuindo o tempo e o custo do tratamento devido a uma menor necessidade de dobras de finalização e reposicionamento de bráquetes. O caso clínico apresentado tem o objetivo de apresentar um tratamento ortodôntico utilizando o método de CI com posicionamento virtual de bráquetes idealizado pelo sistema eXceed™. O posicionamento dos bráquetes programado pelo sistema eXceed™ permitiu um posicionamento "ideal" dos dentes dispensando a implementação de dobras de finalização e proporcionando uma oclusão equilibrada. Essa nova tecnologia demonstrou ser eficiente e eficaz, e o setup ortodôntico fornecido pelo sistema ficou acordante com o resultado final do tratamento ortodôntico (AU)


CAD/CAM systems, including the eXceed™ system, allow orthodontists to perform diagnosis, treatment plan, and brackets virtual positioning. The positioning of each bracket, determined by the software, is transferred to a tridimensional patient model enabling to build an indirect bonding (IB) tray. IB allows improvement in the ideal bracket positioning, decreasing treatment time and cost due to less detailed finishing bends and/or bracket repositioning. The clinical case report purpose is to present an orthodontic treatment using IB with virtual bracket positioning using eXceed™ system. The bracket virtual positioning from eXceed™ system allowed an ideal final teeth positioning eliminating detailed finishing bends and reaching the ideal occlusion as planned. This new technology has shown to be efficient and effective, and the virtual tridimensional orthodontic setup has been in agreement with the final treatment occlusion (AU)


Assuntos
Humanos , Masculino , Adulto , Aparelhos Ortodônticos , Ortodontia Corretiva , Projeto Auxiliado por Computador
7.
Ortho Sci., Orthod. sci. pract ; 12(48): 113-119, 2019. ilus, tab
Artigo em Português | BBO - Odontologia | ID: biblio-1048713

RESUMO

Resumo Introdução: Os sistemas CAD/CAM auxiliam ortodontistas na confecção de alinhadores estéticos e na colagem indireta (posicionamento virtual de bráquetes) em modelos digitais tridimensionais. Este estudo avaliou a precisão e validade do software eXceedTM. Métodos: Vinte modelos de gesso de pacientes foram digitalizados utilizando um escâner de mesa e os arquivos obtidos foram convertidos em estereolitografia pelo software OrthoAnalyserTM. Utilizando os modelos de gesso e digital, seis medidas foram aferidas: Largura Intermolares (LM), Largura Intercaninos (LC), Comprimento do Arco posterior (CA), Diâmetro da Coroa do Pré-Molar (DP), Altura da Coroa do Canino (AC) e Overjet (OJ). Os erros sistemáticos e aleatórios foram avaliados em análises das réplicas das mensurações. As diferenças foram avaliadas usando o teste t de Student. Os erros aleatórios foram quantificados usando o erro do método (V(Sd2/2n) e o Coeficiente de Correlação Intraclasse (ICC). Resultados: Duas das medidas das réplicas em modelos digitais (CA) e de gesso (LC) demostraram erros sistemáticos estatisticamente significativos. O ICC variou de 0,916 a 0,997. Os erros do método foram todos inferiores a 0,41 mm (0,22 mm). Os gráficos de Bland-Altman mostraram que as diferenças de repetitividade entre os dois métodos estavam dentro dos limites de concordância. Os valores DP (0,253 mm), LC (0,396 mm), AC (0,314 mm) e CA (0,359 mm) foram maiores em modelos de gesso do que nos modelos digitais. Conclusão: As medidas realizadas em ambos os métodos foram confiáveis e reprodutíveis, e as medidas dos modelos de gesso foram ligeiramente maiores do que os dos modelos digitais correspondentes (AU)


Abstract Introduction: CAD/CAM systems help orthodontists in the production of aesthetic aligners and in indirect bonding (virtual bracket positioning) in three-dimensional digital models. This study evaluated the accuracy and validity of the eXceedTM 3D software (Roosikrantsi, Tallinn, Estonia). Methods: Twenty patient plaster models were digitized by a desktop scanner, and the files obtained were converted to stereolithography by OrthoAnalyserTM software. Using the cast and digital models, six measurements were performed: Intermolar Width (LM); Intercanine Width (LC); Posterior Arch Length (CA); Premolar Crown Diameter (DP); Canine Crown Hight (AC) and Overjet (OJ). Intra-observer systematic differences between the replicates were described with mean absolute differences and standard errors (SE); differences were assessed using a paired Students t test. Random errors were quantified using the method error statistic (V[Sd2/2n]) and Intraclass Correlation Coefficient (ICC). Differences between methods was evaluated using Students t test. Results: Two of the measurements of the replicas in the digital model (CA) and in the plaster model (LC) showed statistically significant systematic errors. The ICC ranged from 0.916 to 0.997. The method errors were all less than 0.41 mm (0.22 mm). Bland-Altman plots showed that the differences of repeatability between the two methods were within the limits of agreement. The values DP (0.253 mm), LC (0.396 mm), AC (0.314 mm) and CA (0.359 mm) were higher in plaster models than in digital models. Conclusion: The measurements performed in both methods were reliable and reproducible, and plaster models measurements were slightly higher than those of the corresponding digital models. (AU)


Assuntos
Humanos , Masculino , Feminino , Adolescente , Adulto , Ortodontia , Tecnologia Odontológica , Projeto Auxiliado por Computador , Modelos Dentários , Precisão da Medição Dimensional
8.
PLoS Negl Trop Dis ; 12(11): e0006880, 2018 11.
Artigo em Inglês | MEDLINE | ID: mdl-30418969

RESUMO

The recent emergence of Zika virus (ZIKV) in the Americas coincident with increased caseloads of microcephalic infants and Guillain-Barre syndrome has prompted a flurry of research on ZIKV. Much of the research is difficult to compare or repeat because individual laboratories use different virus isolates, growth conditions, and quantitative assays. Here we obtained three readily available contemporary ZIKV isolates and the prototype Ugandan isolate. We generated stocks of each on Vero mammalian cells (ZIKVmam) and C6/36 mosquito cells (ZIKVmos), determined titers by different assays side-by-side, compared growth characteristics using one-step and multi-step growth curves on Vero and C6/36 cells, and examined plaque phenotype. ZIKV titers consistently peaked earlier on Vero cells than on C6/36 cells. Contemporary ZIKV isolates reached peak titer most quickly in a multi-step growth curve when the amplifying cell line was the same as the titering cell line (e.g., ZIKVmam titered on Vero cells). Growth of ZIKVmam on mosquito cells was particularly delayed. These data suggest that the ability to infect and/or replicate in insect cells is limited after growth in mammalian cells. In addition, ZIKVmos typically had smaller, more homogenous plaques than ZIKVmam in a standard plaque assay. We hypothesized that the plaque size difference represented early adaptation to growth in mammalian cells. We plaque purified representative-sized plaques from ZIKVmos and ZIKVmam. ZIKVmos isolates maintained the initial phenotype while plaques from ZIKVmam isolates became larger with passaging. Our results underscore the importance of the cells used to produce viral stocks and the potential for adaptation with minimal cell passages. In addition, these studies provide a foundation to compare current and emerging ZIKV isolates in vitro and in vivo.


Assuntos
Adaptação Fisiológica , Aedes/citologia , Zika virus/crescimento & desenvolvimento , Zika virus/fisiologia , Aedes/virologia , Animais , Linhagem Celular , Humanos , Técnicas In Vitro , Fenótipo , Células Vero , Replicação Viral , Zika virus/classificação , Zika virus/isolamento & purificação
9.
J Tissue Viability ; 27(4): 249-256, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30318397

RESUMO

Burns are injuries caused mainly by thermal trauma, which can progress to unsatisfactory results healing. This study aimed to evaluate the biomaterial (bacterial cellulose membrane) and photobiomodulation, exclusively and associated, in the treatment of third degree burns in rats. Forty male Wistar rats (±280 g) were randomly divided into four groups, with 10 animals each: control group (CG); bacterial cellulose membrane group (MG); laser group (LG) and bacterial cellulose membrane and laser group (MG + L). The burn was caused with a 1 cm2 aluminum plate heated to 150 °C and pressed on the animal's back for 10 s. The treatments were started immediately after induction of injury. For to laser irradiation (660 nm, 100 mW, 25 J/cm2 and energy of 1 J) on five distinct application points were used, on alternate days, a total of five sessions. After ten days of treatment the animals were euthanized for collected samples. One-way ANOVA and Tukey's tests (P < 0.05) were used. Histological analysis revealed differences regarding the healing process phase in each experimental group. MG showed the proliferative phase. The LG demonstrated greater amount of blood vessels and immune expression of VEGF. However, when the treatments were combined, the number of vessels and the immune expression of VEGF factor was lower than LG. Thus, it was concluded that both treatments proposed (biomaterial and LLLT) are good alternatives for third degree burns when applied isolated because they stimulate the healing process by acting on the modulation of the inflammatory phase and promote stimulation of angiogenesis.


Assuntos
Queimaduras/terapia , Celulose/farmacologia , Terapia com Luz de Baixa Intensidade/normas , Cicatrização/efeitos da radiação , Análise de Variância , Animais , Celulose/administração & dosagem , Celulose/uso terapêutico , Ciclo-Oxigenase 2/análise , Modelos Animais de Doenças , Terapia com Luz de Baixa Intensidade/métodos , Masculino , Ratos , Ratos Wistar , Fator A de Crescimento do Endotélio Vascular/análise
10.
Cogn Affect Behav Neurosci ; 18(4): 810-823, 2018 08.
Artigo em Inglês | MEDLINE | ID: mdl-29736679

RESUMO

The present study attempts to better identify the neurophysiological changes occurring during flow experience and how this can be related to the mobilization of attentional resources. Self-reports of flow (using a flow feelings scale) and attention (using thought probes), autonomic activity (heart rate, heart rate variability, and breathing rate), and cerebral oxygenation (using near-infrared spectroscopy) in two regions of the frontoparietal attention network (right lateral frontal cortex and right inferior parietal lobe) were measured during the practice of two simple video games (Tetris and Pong) played at different difficulty conditions (easy, optimal, hard, or self-selected). Our results indicated that an optimal level of difficulty, compared with an easy or hard level of difficulty led to greater flow feelings and a higher concentration of oxygenated hemoglobin in the regions of the frontoparietal network. The self-selected, named autonomy condition did not lead to more flow feelings than the optimal condition; however, the autonomy condition led to greater sympathetic activity (reduced heart rate variability and greater breathing rate) and higher activation of the frontoparietal regions. Our study suggests that flow feelings are highly connected to the mobilization of attentional resources, and all the more in a condition that promotes individuals' choice and autonomy.


Assuntos
Atenção/fisiologia , Lobo Frontal/metabolismo , Lobo Parietal/metabolismo , Adulto , Emoções/fisiologia , Feminino , Frequência Cardíaca/fisiologia , Hemoglobinas/metabolismo , Humanos , Masculino , Percepção/fisiologia , Desempenho Psicomotor/fisiologia , Respiração , Espectroscopia de Luz Próxima ao Infravermelho , Jogos de Vídeo
11.
Artigo em Inglês | MEDLINE | ID: mdl-29753212

RESUMO

The use of performance enhancing drugs is not only common in humans, but also in animal sports, including racing of horses, greyhounds and pigeons. The development of accurate analytical procedures to detect doping agents in sports is crucial in order to protect the fair-play of the game, avoid financial fraud in the attribution of eventual awards and, even more important, to protect the animals from harmful drugs and/or dangerous dosage regimens. The present study aimed to develop and validate, a method that enabled the screening and confirmation of the presence of a beta-agonist (clenbuterol) and three corticosteroids (betamethasone, prednisolone and budesonide) in faeces from pigeons. The extraction procedure entailed the combination of liquid-liquid extraction with solid-phase extraction and the analysis was performed by liquid- chromatography coupled to tandem mass spectrometry, with a single 15 minute chromatographic run-time. The method was validated concerning selectivity, linearity (with coefficients of determination always >0.99), accuracy (87.5-114.9%), inter-day and intra-day precisions, limits of detection (0.14-1.81 ng/g) and limits of quantification (0.49-6.08 ng/g), stability and extraction recovery (71.0%-99.3%). The method was successfully applied for the analysis of samples from two pigeons that had been orally administered betamethasone, demonstrating its suitability for doping control purposes.


Assuntos
Columbidae/metabolismo , Fezes/química , Detecção do Abuso de Substâncias/métodos , Espectrometria de Massas em Tandem/métodos , Corticosteroides/análise , Corticosteroides/metabolismo , Animais , Calibragem , Fracionamento Químico/métodos , Cromatografia Líquida de Alta Pressão/métodos , Clembuterol/análise , Clembuterol/metabolismo , Doping nos Esportes/métodos , Limite de Detecção , Reprodutibilidade dos Testes
12.
Insect Biochem Mol Biol ; 95: 1-9, 2018 04.
Artigo em Inglês | MEDLINE | ID: mdl-29526772

RESUMO

Previously, a Sanger-based sialotranscriptome analysis of adult female Culex tarsalis was published based on ∼2000 ESTs. During the elapsed 7.5 years, pyrosequencing has been discontinued and Illumina sequences have increased considerable in size and decreased in price. We here report an Illumina-based sialotranscriptome that allowed finding the missing apyrase from the salivary transcriptome of C. tarsalis, to determine several full-length members of the 34-62 kDa family, when a single EST has been found previously, in addition to identifying many salivary families with lower expression levels that were not detected previously. The use of multiple libraries including salivary glands and carcasses from male and female organisms allowed for an unprecedented insight into the tissue specificity of transcripts, and in this particular case permitting identification of transcripts putatively associated with blood feeding, when exclusive of female salivary glands, or associated with sugar feeding, when transcripts are found upregulated in both male and female glands.


Assuntos
Culex/metabolismo , Proteínas de Insetos/biossíntese , Caracteres Sexuais , Sialoglicoproteínas/biossíntese , Animais , Feminino , Perfilação da Expressão Gênica , Masculino
13.
Front Immunol ; 9: 543, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29599784

RESUMO

Activated phosphoinositide 3-kinase (PI3K) δ Syndrome (APDS), caused by autosomal dominant mutations in PIK3CD (APDS1) or PIK3R1 (APDS2), is a heterogeneous primary immunodeficiency. While initial cohort-descriptions summarized the spectrum of clinical and immunological manifestations, questions about long-term disease evolution and response to therapy remain. The prospective European Society for Immunodeficiencies (ESID)-APDS registry aims to characterize the disease course, identify outcome predictors, and evaluate treatment responses. So far, 77 patients have been recruited (51 APDS1, 26 APDS2). Analysis of disease evolution in the first 68 patients pinpoints the early occurrence of recurrent respiratory infections followed by chronic lymphoproliferation, gastrointestinal manifestations, and cytopenias. Although most manifestations occur by age 15, adult-onset and asymptomatic courses were documented. Bronchiectasis was observed in 24/40 APDS1 patients who received a CT-scan compared with 4/15 APDS2 patients. By age 20, half of the patients had received at least one immunosuppressant, but 2-3 lines of immunosuppressive therapy were not unusual before age 10. Response to rapamycin was rated by physician visual analog scale as good in 10, moderate in 9, and poor in 7. Lymphoproliferation showed the best response (8 complete, 11 partial, 6 no remission), while bowel inflammation (3 complete, 3 partial, 9 no remission) and cytopenia (3 complete, 2 partial, 9 no remission) responded less well. Hence, non-lymphoproliferative manifestations should be a key target for novel therapies. This report from the ESID-APDS registry provides comprehensive baseline documentation for a growing cohort that will be followed prospectively to establish prognostic factors and identify patients for treatment studies.


Assuntos
Síndromes de Imunodeficiência/tratamento farmacológico , Imunossupressores/uso terapêutico , Sistema de Registros , Sirolimo/uso terapêutico , Adolescente , Adulto , Criança , Pré-Escolar , Classe I de Fosfatidilinositol 3-Quinases , Europa (Continente) , Humanos , Pessoa de Meia-Idade , Sociedades Médicas , Adulto Jovem
15.
Biomed Mater Eng ; 29(1): 29-42, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29254071

RESUMO

INTRODUCTION: Burn injuries represent a high risk of morbidity and mortality. The wound healing process is complex and requires the participation of different types of cells. Therefore, new biomaterials, which innovate the wound healing process, are being investigated. OBJECTIVE: The aim of this study was to investigate the use of bacterial cellulose both in its pure state and enriched with lidocaine in full-thickness burns in rats. METHODS: Thirty rats (Wistar) (260 ± 20 gramas) divided into control group (CG), bacterial cellulose membrane group (MG) and bacterial cellulose membrane enriched with lidocaine group (MLG) were used. The burns were induced using a 150°C heated soldering iron, held on the animal neck for 10 seconds. The biomaterial was applied immediately after injury and skin samples were collected on the tenth day of the treatment. The level of significance of p⩽0.05 was used for the conclusion of the statistical analysis. RESULTS: The groups treated with the biomaterials, a histological pattern compatible with a more advanced repair stage showing skin appendages, mild inflammatory infiltrate, better collagen fiber organization and mild immunostaining COX-2 and MMP-9 was observed, when compared to the control group that did not receive any type of treatment. CONCLUSION: Thus, was concluded that the bacterial cellulose-based biomaterial both in its pure state and enriched with lidocaine optimizing the full-thickness burn wound healing in rats.


Assuntos
Materiais Biocompatíveis/uso terapêutico , Curativos Biológicos , Queimaduras/terapia , Celulose/uso terapêutico , Polissacarídeos Bacterianos/uso terapêutico , Anestésicos Locais/uso terapêutico , Animais , Materiais Biocompatíveis/química , Queimaduras/patologia , Celulose/química , Lidocaína/uso terapêutico , Masculino , Membranas Artificiais , Polissacarídeos Bacterianos/química , Ratos Wistar , Pele/efeitos dos fármacos , Pele/patologia , Cicatrização/efeitos dos fármacos
16.
Clin Biomech (Bristol, Avon) ; 44: 83-89, 2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-28364674

RESUMO

BACKGROUND: There is a frequent complaint about balance problems among fibromyalgia syndrome patients; however, there are not enough studies that have shown static postural sway of women with fibromyalgia syndrome. This study aimed to compare static postural sway of women with and without fibromyalgia syndrome. METHODS: This is a cross-sectional study in which twenty-nine women with fibromyalgia syndrome and 20 without took part. A posturography evaluation was performed in six different situations (bipedal, right tandem and left tandem, with eyes opened and closed), and questionnaires for clinical depression symptoms, clinical anxiety symptoms, sleep quality, and Visual Analogue Scales for Pain and Fatigue were applied. Mann-Whitney U test was used to check differences among groups; Wilcoxon matched-pair test was used to check differences intragroup; Cohen d coefficient was used to measure effect sizes and Pearson Correlation Coefficient was used for correlations among variables. Level of significance adopted was 5%. FINDINGS: Women with fibromyalgia syndrome have presented worse postural sway than women without fibromyalgia syndrome in all situations (P<0.05), and worse scores in all questionnaires (P<0.05). In the eyes closed situations, women with fibromyalgia syndrome presented worse postural sway than women without in the same conditions. INTERPRETATION: Women with fibromyalgia syndrome have worse performance in the static posture test, more prominent in reduced support bases with eyes closed. Pain, fatigue, depression and anxiety may have directly influenced postural sway in fibromyalgia syndrome patients.


Assuntos
Fibromialgia/fisiopatologia , Equilíbrio Postural/fisiologia , Adulto , Estudos Transversais , Fadiga/fisiopatologia , Feminino , Humanos , Pessoa de Meia-Idade , Dor/fisiopatologia , Medição da Dor , Inquéritos e Questionários
17.
PLoS Negl Trop Dis ; 11(2): e0005394, 2017 02.
Artigo em Inglês | MEDLINE | ID: mdl-28187142

RESUMO

Half of the human population is at risk of infection by an arthropod-borne virus. Many of these arboviruses, such as West Nile, dengue, and Zika viruses, infect humans by way of a bite from an infected mosquito. This infectious inoculum is insect cell-derived giving the virus particles distinct qualities not present in secondary infectious virus particles produced by infected vertebrate host cells. The insect cell-derived particles differ in the glycosylation of virus structural proteins and the lipid content of the envelope, as well as their induction of cytokines. Thus, in order to accurately mimic the inoculum delivered by arthropods, arboviruses should be derived from arthropod cells. Previous studies have packaged replicon genome in mammalian cells to produce replicon particles, which undergo only one round of infection, but no studies exist packaging replicon particles in mosquito cells. Here we optimized the packaging of West Nile virus replicon genome in mosquito cells and produced replicon particles at high concentration, allowing us to mimic mosquito cell-derived viral inoculum. These particles were mature with similar genome equivalents-to-infectious units as full-length West Nile virus. We then compared the mosquito cell-derived particles to mammalian cell-derived particles in mice. Both replicon particles infected skin at the inoculation site and the draining lymph node by 3 hours post-inoculation. The mammalian cell-derived replicon particles spread from the site of inoculation to the spleen and contralateral lymph nodes significantly more than the particles derived from mosquito cells. This in vivo difference in spread of West Nile replicons in the inoculum demonstrates the importance of using arthropod cell-derived particles to model early events in arboviral infection and highlights the value of these novel arthropod cell-derived replicon particles for studying the earliest virus-host interactions for arboviruses.


Assuntos
Aedes/virologia , Infecções por Arbovirus/virologia , Arbovirus/fisiologia , Insetos Vetores/virologia , Replicon , Febre do Nilo Ocidental/virologia , Vírus do Nilo Ocidental/genética , Animais , Arbovirus/genética , Feminino , Humanos , Camundongos , Camundongos Endogâmicos C57BL , Proteínas Estruturais Virais/genética , Proteínas Estruturais Virais/metabolismo , Montagem de Vírus , Cultura de Vírus , Vírus do Nilo Ocidental/crescimento & desenvolvimento , Vírus do Nilo Ocidental/fisiologia
18.
Microsc Res Tech ; 79(4): 313-20, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26853699

RESUMO

Burns are injuries caused by direct or indirect contact to chemical, physical, or biological agents. Low-level laser therapy (LLLT) is a promising treatment since it is low-cost, non-invasive, and induces cell proliferation. This study aimed to investigate the effects of LLLT (660 nm) at two different fluences (12.5 J/cm(2) and 25 J/cm(2) ) per point of application on third-degree burns in rats. Thirty rats (Wistar) divided into GC, GL12.5, and GL25 were used in the study, and submitted to burn injury through a soldering iron at 150°C, pressed on their back for 10 s. LLLT was applied immediately, and 2, 4, 6, and 8 days after wound induction. Histological analysis revealed a decreased inflammatory infiltrate in the group treated with 25 J/cm(2) , and intense inflammatory infiltrate in the control group and in the group treated with 12.5 J/cm(2) . The immunostaining of COX-2 was more intense in the control groups and in the group treated with 12.5 J/cm(2) than in the group treated with 25 J/cm(2) . Conversely, VEGF immunomarking was more expressive in the group treated with 25 J/cm(2) than it was in the other two groups. Therefore, our findings suggest that the use of 25 J/cm(2) and 1 J of energy was more effective in stimulating the cellular processes involved in tissue repair on third-degree burns in rats by reducing the inflammatory phase, and stimulating angiogenesis, thus restoring the local microcirculation which is essential for cell migration.


Assuntos
Queimaduras/terapia , Terapia com Luz de Baixa Intensidade/métodos , Animais , Queimaduras/patologia , Queimaduras/fisiopatologia , Modelos Animais de Doenças , Humanos , Terapia com Luz de Baixa Intensidade/instrumentação , Masculino , Ratos , Ratos Wistar , Cicatrização/efeitos da radiação
19.
In. Santos, Elizabete Silva dos; Trindade, Pedro Henrique Duccini Mendes; Moreira, Humberto Graner. Tratado Dante Pazzanese de emergências cardiovasculares. São Paulo, Atheneu, 2016. p.921-933, ilus, tab.
Monografia em Português | Sec. Est. Saúde SP, SESSP-IDPCPROD, Sec. Est. Saúde SP | ID: ses-33170
20.
Sci Rep ; 5: 14761, 2015 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-26434597

RESUMO

The prospect of carbon-based magnetic materials is of immense fundamental and practical importance, and information on atomic-scale features is required for a better understanding of the mechanisms leading to carbon magnetism. Here we report the first direct detection of the microscopic magnetic field produced at (13)C nuclei in a ferromagnetic carbon material by zero-field nuclear magnetic resonance (NMR). Electronic structure calculations carried out in nanosized model systems with different classes of structural defects show a similar range of magnetic field values (18-21 T) for all investigated systems, in agreement with the NMR experiments. Our results are strong evidence of the intrinsic nature of defect-induced magnetism in magnetic carbons and establish the magnitude of the hyperfine magnetic field created in the neighbourhood of the defects that lead to magnetic order in these materials.

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