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1.
Med Image Anal ; 67: 101874, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-33166771

RESUMO

Automated detection of curvilinear structures, e.g., blood vessels or nerve fibres, from medical and biomedical images is a crucial early step in automatic image interpretation associated to the management of many diseases. Precise measurement of the morphological changes of these curvilinear organ structures informs clinicians for understanding the mechanism, diagnosis, and treatment of e.g. cardiovascular, kidney, eye, lung, and neurological conditions. In this work, we propose a generic and unified convolution neural network for the segmentation of curvilinear structures and illustrate in several 2D/3D medical imaging modalities. We introduce a new curvilinear structure segmentation network (CS2-Net), which includes a self-attention mechanism in the encoder and decoder to learn rich hierarchical representations of curvilinear structures. Two types of attention modules - spatial attention and channel attention - are utilized to enhance the inter-class discrimination and intra-class responsiveness, to further integrate local features with their global dependencies and normalization, adaptively. Furthermore, to facilitate the segmentation of curvilinear structures in medical images, we employ a 1×3 and a 3×1 convolutional kernel to capture boundary features. Besides, we extend the 2D attention mechanism to 3D to enhance the network's ability to aggregate depth information across different layers/slices. The proposed curvilinear structure segmentation network is thoroughly validated using both 2D and 3D images across six different imaging modalities. Experimental results across nine datasets show the proposed method generally outperforms other state-of-the-art algorithms in various metrics.

2.
Small ; : e2005336, 2020 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-33236828

RESUMO

Highly stretchable, conductive, biocompatible conductors, and connectors are crucial for the fabrication of flexible devices. However, it remains a problem to get highly stretchable, conductive materials with low cost on a large scale. Another problem in production is the connection between soft and rigid components. Here, a new conductive nanocomposite is reported by mixing the 11-mercaptoundecanoic acid (MUA) modified liquid metal (LM) nanoparticles with polystyrene-block-polybutadiene-block-polystyrene (SBS), which is biocompatible (in vivo and in vitro), conductive (12 000 S cm-1 of conductivity), and stretchable (800% of elongation). Apart from its good performance, this material can be produced on a large scale by using a commercial polymer product and a straightforward physical production process. MUA is used to compromise the dense "gallium oxide shell" of liquid metal nanoparticles such that the whole composite can become conductive. By using resin to modify this composite, this new conductive material can be adhesive and highly conductive, and serve as a stable and efficient connector between soft conductor and rigid component.

3.
ACS Nano ; 2020 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-33030886

RESUMO

Intimal hyperplasia (IH) in vein grafts (VGs) is a major issue in coronary artery bypass grafting (CABG) surgery. Although external stents can attenuate IH of VGs to some extent, none of the existing external stents have shown satisfactory clinical outcomes. Here we develop a flexible, biodegradable, and conductive external metal-polymer conductor stent (MPCS) that can electroporate the vessel wall and produce a protein that prevents IH. We designed the plasmid DNA encoding the tissue inhibitor of metalloproteinases-3 (TIMP-3) and lyophilized it on the inner surface of the MPCS to deliver into the adventitia and the middle layer of VGs for gene therapy. Coupled with its continuous mechanical support to prevent dilation after implanting, the MPCS can inhibit the IH of VGs significantly in the rabbit model. This proof-of-concept demonstration may aid the development of other implantable bioelectronics for electroporation gene therapy.

4.
Annu Int Conf IEEE Eng Med Biol Soc ; 2020: 1360-1363, 2020 07.
Artigo em Inglês | MEDLINE | ID: mdl-33018241

RESUMO

Registration of multimodal retinal images is of great importance in facilitating the diagnosis and treatment of many eye diseases, such as the registration between color fundus images and optical coherence tomography (OCT) images. However, it is difficult to obtain ground truth, and most existing algorithms are for rigid registration without considering the optical distortion. In this paper, we present an unsupervised learning method for deformable registration between the two images. To solve the registration problem, the structure achieves a multi-level receptive field and takes contour and local detail into account. To measure the edge difference caused by different distortions in the optics center and edge, an edge similarity (ES) loss term is proposed, so loss function is composed by local cross-correlation, edge similarity and diffusion regularizer on the spatial gradients of the deformation matrix. Thus, we propose a multi-scale input layer, U-net with dilated convolution structure, squeeze excitation (SE) block and spatial transformer layers. Quantitative experiments prove the proposed framework is best compared with several conventional and deep learningbased methods, and our ES loss and structure combined with Unet and multi-scale layers achieve competitive results for normal and abnormal images.


Assuntos
Algoritmos , Retina , Fundo de Olho , Retina/diagnóstico por imagem , Tomografia de Coerência Óptica
5.
Biosens Bioelectron ; 166: 112444, 2020 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-32758910

RESUMO

How to balance the sensitivity and signal-to-noise ratio of immunosensor remains many challenges during various diseases diagnosis. Here we develop a new microfluidic immunosensor based on surface-modified mesoporous nanofibers, and simultaneously realize an ultra-sensitivity and high signal-to-noise ratio for the detection of multiple biomarkers. In the current study, we fabricated titanium dioxide (TiO2)-based mesoporous electrospinning nanofibers, and modified nanofiber surface with both octadecylphosphonic acid (OPA) and poly(ethylene oxide)-poly(propylene oxide) triblock copolymer (PEO-PPO-PEO). Such nanofibers as solid substrate are covered on microfluidic channels. The porosity of our nanofibers dramatically increased the adsorption capability of antibodies, realizing an ultra sensitivity of biomarker detection. PEO-PPO-PEO modification can significantly block non-specific absorptions, obtaining a satisfied signal-to-noise ratio. For the detection of HIV p24 and interleukin 5 (IL-5), our immunosensor increased 6.41 and 6.93 fold in sensitivity and improved 504.66% and 512.80% in signal-to-noise ratio, in compared with gold standard immunoassay (ELISA) used in the clinic. Our immunosensor also broaden the linear range for the detection of HIV p24 (0.86-800 pg/ml) and IL-5 (0.70-800 pg/ml), in compared with ELISA which is 5.54-500 pg/ml for HIV p24 and 4.84-500 pg/ml for IL-5. Our work provided a guideline for the construction of advanced point-of-care immunosensor with an ultra-sensitivity and high signal-to-noise ratio for disease diagnosis.

6.
Minerva Med ; 2020 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-32406219

RESUMO

BACKGROUND: To investigate the expression and clinical value of miRNA-128 (miR-128) and insulin-like growth factor-1 (IGF- 1) in patients with acute ischemic stroke (AIS). METHODS: 80 patients with acute ischemic stroke hospitalized in Rizhao Hospital of TCM from August 2016 to August 2018 were selected as the experimental group. 60 healthy patients with normal physical examination during the same period were selected as the control group. The expression levels of miR-128 and IGF-1 were compared between the two groups and between AIS patients with different levels of nervous functional defects, different infarct size and different prognosis. ROC curve of serum miR-128 and IGF-1 expressions for AIS diagnosis alone and in combination was analyzed. RESULTS: The expression level of miR-128 in the serum of patients in the experimental group was higher than that of the control group, and the expression level of IGF-1 was lower than that of the control group (p < 0.001). The serum miR-128 expression of patients in the ineffective group was higher than that of the effective group, and the expression of IGF-1 was lower than that of the effective group (p < 0.001). The expression level of serum miR-128 in AIS patients was higher than that in healthy subjects and increased with the aggravation of the disease. The expression level of IGF-1 of AIS patients was lower than that of healthy subjects and decreased with the aggravation of the disease. The two factors were involved in the formation and deterioration of the disease. CONCLUSIONS: The expression levels can reflect the severity of the disease to a certain extent, and provide references for the early diagnosis and prognostic treatment in patients with acute ischemic stroke.

7.
IEEE Trans Med Imaging ; 39(5): 1392-1403, 2020 05.
Artigo em Inglês | MEDLINE | ID: mdl-31675323

RESUMO

The detection of retinal vessel is of great importance in the diagnosis and treatment of many ocular diseases. Many methods have been proposed for vessel detection. However, most of the algorithms neglect the connectivity of the vessels, which plays an important role in the diagnosis. In this paper, we propose a novel method for retinal vessel detection. The proposed method includes a dense dilated network to get an initial detection of the vessels and a probability regularized walk algorithm to address the fracture issue in the initial detection. The dense dilated network integrates newly proposed dense dilated feature extraction blocks into an encoder-decoder structure to extract and accumulate features at different scales. A multi-scale Dice loss function is adopted to train the network. To improve the connectivity of the segmented vessels, we also introduce a probability regularized walk algorithm to connect the broken vessels. The proposed method has been applied on three public data sets: DRIVE, STARE and CHASE_DB1. The results show that the proposed method outperforms the state-of-the-art methods in accuracy, sensitivity, specificity and also area under receiver operating characteristic curve.

8.
J Med Genet ; 57(2): 73-81, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-31484719

RESUMO

Parkinson's disease (PD) is a movement disorder identified more than 200 years ago; today it is defined by specific motor symptoms that together receive the name of parkinsonism. PD diagnosis is reached with the full parkinsonian syndrome, but in recent years, a series of non-motor symptoms have arisen as intrinsic components of PD. These non-motor symptoms are variable, creating a widely heterogenous disease presentation. Some non-motor symptoms appear in late disease stages and are explained as the natural progression of PD pathology into other brain centres, including the frontal cortex. Other symptoms can appear a decade or earlier preceding PD diagnosis, particularly hyposmia (loss of smell) and constipation. These early symptoms and the accompanying protein pathology have stimulated a lively conversation about the origin and nature of PD and other related conditions: some authors propose that PD starts in the olfactory mucosa and the gut due to direct exposure to toxins or pathogens. This pathology then travels by anatomically interconnected networks to the midbrain to cause motor symptoms and the cortex to cause late complications. Other models propose that PD develops in multiple independent foci that do not require pathology spread. We will review these hypotheses in the context of recent developments regarding the spread of amyloids and propose a mixed model where a multifocal origin explains the variable presentation of PD, while cell-to-cell spread explains stereotypical disease progression.

9.
Onco Targets Ther ; 12: 8125-8138, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31686858

RESUMO

Background: Upregulation of CXCL8 (C-X-C motif ligand 8) in tumor cells has been reported in several types of cancer, and it correlates with a poor prognosis. However, the role of CXCL8 in glioma progression remains unknown. Materials and methods: In this study, we examined CXCL8 expression levels in human glioma cell lines and in sixteen human gliomas with different grades. The molecular role of CXCL8 in glioma cells was investigated using quantitative polymerase chain reaction (qRT-PCR) assays, Western blotting, CCK-8 assays, EdU assays, colony formation assays, Transwell migration and invasion assays. Results: We found that high expression levels of CXCL8 were positively associated with progression and poor prognosis in human glioma. Mechanistically, CXCL8 promoted the epithelial-mesenchymal transition (EMT) in glioma cells by activating the JAK/STAT1/HIF-1α/Snail signaling pathway. Conclusion: Taken together, our data provide a plausible mechanism for CXCL8-modulated glioma progression, which suggests that CXCL8 may represent a potential therapeutic target in the prevention and treatment of gliomas.

10.
Lab Chip ; 19(16): 2750-2757, 2019 08 21.
Artigo em Inglês | MEDLINE | ID: mdl-31338499

RESUMO

Point-of-care (POC) medical assays provide critical information to guide clinical therapy for a broad range of medical scenarios, such as resource-poor settings and specialty departments in hospitals. Even though many types of POC assays can be done in automated devices, these POC assays typically cannot well accommodate the multiplexed detection of biomarkers where a large dynamic range is needed. Here, we report a POC assay, which is both automated and suitable for detecting multiple biomarkers with dynamic detection ranges. We call it a dynamic multiplexed immunoassay (DMI). We control the concentrations of capture antibodies and the intensity of the readout signal to dynamically modulate the detection range of immunoassays (pg mL-1 to µg mL-1), leading to the multiplexed detection of C-reactive protein (CRP), procalcitonin (PCT), and interleukin 6 (IL-6) simultaneously in undiluted human serum samples. The POC assay allows the rapid and accurate detection of infection in patients.


Assuntos
Proteína C-Reativa/análise , Imunoensaio/instrumentação , Interleucina-6/sangue , Análise em Microsséries/instrumentação , Sistemas Automatizados de Assistência Junto ao Leito , Pró-Calcitonina/sangue , Biomarcadores/sangue , Humanos , Microscopia Eletrônica de Varredura/instrumentação
11.
Biomed Microdevices ; 21(3): 69, 2019 07 04.
Artigo em Inglês | MEDLINE | ID: mdl-31273551

RESUMO

Microfluidics has shown its vitality in scientific research. But the lack of fast and straightforward approaches for aligning chip and easy-to-control on-chip valve still prevent microfluidic chips from becoming powerful commercial products. This work presents an aligner based on hinge structures, which we call a "hinge aligner", for aligning microfluidic chips. Two flat chip holders are connected by a connecting rod so that the chip holders can rotate relative to each other along the connecting rod, in the way a hinge works. The two chip holders contain pre-designed recesses for placing two chips which can align chips with 20 µm resolution. Meanwhile, with this hinge aligner, we can easily implement a fully sealed on-chip valve, which can prevent aqueous liquids from leaking even at 80 °C for 30 min. The real immunoassay result shows aligned microfluidic chips can detect protein with improved reproducibility in both high and low concentration of biomarkers.


Assuntos
Dispositivos Lab-On-A-Chip , Fosfatase Alcalina/sangue , Imunoensaio , Limite de Detecção , Sistemas Automatizados de Assistência Junto ao Leito , Fatores de Tempo
12.
Int J Immunopathol Pharmacol ; 33: 2058738419866021, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31359794

RESUMO

Ginsenoside Rb1 (Rb1) possesses a cardioprotective effect via mediating microRNAs (miRs), while it is unexplored whether miR-210 is regulated by Rb1 in response to oxidative stress. Human endothelial EA.hy926 cells were stimulated with H2O2 before Rb1 treatment. After transfection, cell viability, apoptosis, migration, and invasion assays were conducted. Western blot was applied to quantify protein. BCL2/adenovirus E1B 19-kDa interacting protein 3 (BNIP3) and miR-210 were analyzed with quantitative reverse transcription polymerase chain reaction. Dual luciferase activity assay was performed. Rb1 elevated viability, migration, and invasion of H2O2-treated cells. H2O2-induced apoptosis was moderated by Rb1. miR-210 was augmented in H2O2-treated cells after Rb1 stimulation. miR-210 inhibitor abolished the positive effects of Rb1. BNIP3 was negatively modulated by miR-210 and implicated in modulating viability, apoptosis, and migration and invasion. In addition, BNIP3 modulated phosphorylation of regulators. Rb1 repressed oxidative injury via elevating miR-210. miR-210 negatively mediated BNIP3, which participated in oxidative damage via regulating mammalian targets of rapamycin (mTOR) and nuclear factor-κB (NF-κB).


Assuntos
Ginsenosídeos/farmacologia , Peróxido de Hidrogênio/farmacologia , MicroRNAs/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Substâncias Protetoras/farmacologia , Apoptose/efeitos dos fármacos , Linhagem Celular , Movimento Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Células Endoteliais , Humanos , Proteínas de Membrana/metabolismo , NF-kappa B/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Serina-Treonina Quinases TOR/metabolismo
13.
Anal Chim Acta ; 1060: 133-141, 2019 Jul 04.
Artigo em Inglês | MEDLINE | ID: mdl-30902327

RESUMO

This report demonstrates that a microfluidic device with integrated silicon filter exhibits outstanding capture efficiency and superior enrichment purity when employed to separate tumor cells from whole blood samples. We fabricate the silicon filter with pyramidal microcavity array (MCA) by microfabrication. We design the structure of the cavity to efficiently enrich tumor cells, while allowing hematologic cells to deform and pass through. The capture efficiency of MCF-7, SW620 and Hela cells spiked in 1 mL of whole blood are approximately 80%. Unwanted white blood cells (WBCs) trapped on the MCA are below 0.003%. In addition, this microfluidic device successfully identifies circulating tumor cells (CTCs) in 5 of 6 patients' blood samples, with a range of 5-86 CTCs per mL. These results reveal that the disposable microfluidic device can effectively enrich tumor cells with different sizes and various morphologies, while maintaining high capture efficiency and purity. Therefore, this label-free technique can serve as a versatile platform to facilitate CTCs analysis in diverse biochemical applications.


Assuntos
Separação Celular , Técnicas Analíticas Microfluídicas , Células Neoplásicas Circulantes/patologia , Desenho de Equipamento , Humanos , Técnicas Analíticas Microfluídicas/instrumentação , Microscopia de Fluorescência , Silício/química , Células Tumorais Cultivadas
14.
Curr Microbiol ; 76(4): 495-502, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30798378

RESUMO

Bacterial strain 71-2 with phosphate-solubilizing activity was isolated from tobacco rhizosphere and classified as Burkholderia cenocepacia based on sequence analyses of 16S rRNA and recA genes. To learn phosphate-solubilizing mechanisms of 71-2, mutants showing reduced solubilizing phosphate activity were obtained using the EZ-Tn5 transposon. Mutant 71-2-MT51 was reduced in the solubilizing phosphate content to 34.36% as compared with the wild-type strain 71-2. The disrupted gene in 71-2-MT51 was cloned and sequenced, and the putative protein from the gene shared 65.26% identity to protein sequences of enolase from Escherichia coli, which suggests the gene encodes an enzyme of enolase. Complementation analyzing showed that Eno was responsible for phosphate solubilizing for B. cenocepacia strain 71-2. To our knowledge, this is the first report of Eno involved in phosphate solubilizing in B. cenocepacia as well as in other bacteria.


Assuntos
Proteínas de Bactérias/genética , Burkholderia cenocepacia/genética , Burkholderia cenocepacia/metabolismo , Fosfatos/metabolismo , Fosfopiruvato Hidratase/genética , Proteínas de Bactérias/metabolismo , Burkholderia cenocepacia/classificação , Burkholderia cenocepacia/crescimento & desenvolvimento , DNA Bacteriano/genética , Teste de Complementação Genética , Mutagênese , Mutação , Fosfopiruvato Hidratase/metabolismo , Filogenia , RNA Ribossômico 16S/genética , Recombinases Rec A/genética , Rizosfera , Análise de Sequência de DNA , Microbiologia do Solo , Tabaco/microbiologia
15.
ACS Appl Mater Interfaces ; 11(7): 7138-7147, 2019 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-30681826

RESUMO

Recently, great stretchability progress has been witnessed in elastic electronics. However, such electronics are costly, toxic, or cannot pattern on a broad range of substrates, which limit their large-scale fabrications and applications. Here, to overcome those limitations, an ink comprising liquid metal particles and desirable polymer solutions is developed. The polymer solutions in our ink can be adjusted to print on different surfaces and avoid toxic organic solvents in most cases. The ink can be sintered by a small strain (∼10%) in room temperature. Using our ink, conductors with high stretchability (380000 S/m at a strain of 1000%) can be printed in low consumption (liquid metal consumption 3.27 mg/cm2), in a large area (bestrew the entire surface of a T-shirt), and in high throughputs (∼105 cm2/h). The ink can be printed on a T-shirt to achieve a smart wearable platform that integrates electronics for strain/electrophysiology/electrochemistry detection and temperature monitoring/controlling.

16.
Adv Mater ; 31(45): e1805033, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30345586

RESUMO

The rapid development of microfluidics technology has promoted new innovations in materials science, particularly by interacting with biological systems, based on precise manipulation of fluids and cells within microscale confinements. This article reviews the latest advances in microfluidics-based biomaterials and biodevices, highlighting some burgeoning areas such as functional biomaterials, cell manipulations, and flexible biodevices. These areas are interconnected not only in their basic principles, in that they all employ microfluidics to control the makeup and morphology of materials, but also unify at the ultimate goals in human healthcare. The challenges and future development trends in biological application are also presented.


Assuntos
Materiais Biocompatíveis , Dispositivos Lab-On-A-Chip , Sistemas de Liberação de Medicamentos/instrumentação , Humanos , Fenômenos Mecânicos , Análise Serial de Tecidos/instrumentação
17.
Anal Chem ; 90(19): 11423-11430, 2018 10 02.
Artigo em Inglês | MEDLINE | ID: mdl-30191718

RESUMO

Stimuli-responsive hydrogels (SRhG) that undergo response to physicochemical stimuli have been broadly applied in separation, biosensing, and drug delivery. Since, most of the SRhG are based on the structural behaviors (swelling or collapse). Herein, we describe a more simple and convenient colorimetric SRhG of polydopamine-coated gold nanoparticles (Au@PDA NPs) hydrogel. The newly developed SRhG is based on the in situ surface chemistry of Au@PDA NPs with core-shell structure embedding in agarose hydrogel. Silver ions can in situ form Ag NPs on surfaces of Au@PDA NPs (Ag_Au@PDA NPs with core-satellites like structure) at ambient conditions, which shift the localized surface plasmon resonance (LSPR) absorption peak and result in color change. The solid sensing phase of SRhG shows greatly improved stability and anti-interference ability comparing to that of solution phase sensing. With rational designs, Au@PDA NPs hydrogel shows great potential in optical sensing, for example, biothiol detection, and coupled with enzyme-cascade reaction for acetylcholinesterase activity detection and inhibitor assays with excellent sensitivity and selectivity.


Assuntos
Ouro/química , Hidrogéis/química , Indóis/química , Nanopartículas Metálicas/química , Polímeros/química , Acetilcolinesterase/química , Acetilcolinesterase/metabolismo , Inibidores da Colinesterase/química , Inibidores da Colinesterase/metabolismo , Colorimetria , Glutationa/análise , Prata/química , Compostos de Sulfidrila/análise , Ressonância de Plasmônio de Superfície
18.
iScience ; 4: 302-311, 2018 Jun 29.
Artigo em Inglês | MEDLINE | ID: mdl-30240749

RESUMO

Stretchable, biocompatible devices can bridge electronics and biology. However, most stretchable conductors for such devices are toxic, costly, and regularly break/degrade after several large deformations. Here we show printable, highly stretchable, and biocompatible metal-polymer conductors by casting and peeling off polymers from patterned liquid metal particles, forming surface-embedded metal in polymeric hosts. Our printable conductors present good stretchability (2,316 S/cm at a strain of 500%) and repeatability (ΔR/R <3% after 10,000 cycles), which can satisfy most electrical applications in extreme deformations. This strategy not only overcomes large surface tension of liquid metal but also avoids the undesirable sintering of its particles by stress in deformations, such that stretchable conductors can form on various substrates with high resolution (15 µm), high throughput (∼2,000 samples/hour), and low cost (one-quarter price of silver). We use these conductors for stretchable circuits, motion sensors, wearable glove keyboards, and electroporation of live cells.

19.
Lab Chip ; 17(13): 2225-2234, 2017 06 27.
Artigo em Inglês | MEDLINE | ID: mdl-28573279

RESUMO

Microfluidic platforms capable of automated, rapid, sensitive, and quantitative detection of biomarkers from patient samples could make a major impact on clinical or point-of-care (POC) diagnosis. In this work, we realize an automated diagnostic platform composed of two main components: (1) a disposable, self-contained, and integrated microfluidic chip and (2) a portable instrument that carries out completely automated operations. To demonstrate its potential for real-world application, we use injection molding for mass fabrication of the main components of disposable microfluidic chips. The assembled three-layered chip with on-chip mechanical valves for fluid control consists of (1) a top silicone fluidic layer with embedded zigzag microchannels, reagent reservoirs and a negative pressure port, (2) a middle tinfoil layer with patterned antibody/antigen stripes, and (3) a bottom silicone substrate layer with waste reservoirs. The versatility of the microfluidics-based system is demonstrated by implementation of a chemiluminescence immunoassay for quantitative detection of C-reactive protein (CRP) and testosterone in real clinical samples. This lab-on-a-chip platform with features of quantitation, portability and automation provides a promising strategy for POC diagnosis.


Assuntos
Automação Laboratorial/instrumentação , Biomarcadores/sangue , Dispositivos Lab-On-A-Chip , Medições Luminescentes/instrumentação , Técnicas Analíticas Microfluídicas/instrumentação , Humanos , Medições Luminescentes/métodos , Técnicas Analíticas Microfluídicas/métodos , Testosterona/sangue
20.
Adv Healthc Mater ; 6(15)2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28322517

RESUMO

Conventional immunoassays suffer from at least one of these following limitations: long processing time, high costs, poor user-friendliness, technical complexity, poor sensitivity and specificity. Microfluidics, a technology characterized by the engineered manipulation of fluids in channels with characteristic lengthscale of tens of micrometers, has shown considerable promise for improving immunoassays that could overcome these limitations in medical diagnostics and biology research. The combination of microfluidics and immunoassay can detect biomarkers with faster assay time, reduced volumes of reagents, lower power requirements, and higher levels of integration and automation compared to traditional approaches. This review focuses on the materials-related aspects of the recent advances in microfluidics-based immunoassays for point-of-care (POC) diagnostics of biomarkers. We compare the materials for microfluidic chips fabrication in five aspects: fabrication, integration, function, modification and cost, and describe their advantages and drawbacks. In addition, we review materials for modifying antibodies to improve the performance of the reaction of immunoassay. We also review the state of the art in microfluidic immunoassays POC platforms, from the laboratory to routine clinical practice, and also commercial products in the market. Finally, we discuss the current challenges and future developments in microfluidic immunoassays.


Assuntos
Materiais Biocompatíveis/química , Técnicas Biossensoriais/instrumentação , Imunoensaio/instrumentação , Técnicas Analíticas Microfluídicas/instrumentação , Microfluídica/instrumentação , Sistemas Automatizados de Assistência Junto ao Leito , Desenho de Equipamento , Análise de Falha de Equipamento , Humanos , Reprodutibilidade dos Testes , Sensibilidade e Especificidade
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