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1.
J Dairy Sci ; 103(8): 7411-7415, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32534928

RESUMO

This study assessed potential risk factors associated with introduction of Mycobacterium avium ssp. paratuberculosis (MAP) into dairy cattle herds in the Galicia region, northwestern Spain. The study was carried out with data collected from 93 dairies enrolled in a voluntary MAP control program. Information on potential risk factors was obtained through personal interviews with the farmers and veterinarians in charge of the control program of each farm. In addition, blood samples were taken annually over 2 years from cows on the farms in the program, and analyzed with a commercial ELISA to detect antibodies to MAP. Fecal samples of all ELISA-positive cows were analyzed using PCR. Based on χ2 test and Fisher's exact test, purchase practices, shared manure truck, shared materials, and visitors per month who contacted animals were found to be significantly associated with farm MAP infection status. Multiple logistic regression indicated that purchase practices and herd size (included as a potential confounder) are the variables that best predict MAP status.

2.
J Dairy Sci ; 103(7): 6454-6472, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32359990

RESUMO

A quantitative risk assessment model was developed to estimate the annual probability of introducing bovine viral diarrhea virus (BVDV) and bovine herpesvirus 1 (BoHV-1) at the farm level through animal movements. Data from 2017 official animal movements, biosecurity questionnaires, scientific literature, and expert opinion from field veterinarians were taken into consideration for model input parameters. Purchasing or introducing cattle, rearing replacement heifers offsite, showing cattle at competitions, sharing transport vehicles with other herds, and transporting cattle in vehicles that have not been cleaned and disinfected were considered in the model. The annual probability of introducing BVDV or BoHV-1 through infected animals was very heterogeneous between farms. The median likelihoods of BVDV and BoHV-1introduction were 12 and 9%, respectively. Farms that purchased cattle from within their region (i.e., local movements) and shared transport with other farms had a higher probability for BVDV and BoHV-1 introduction. This model can be a useful tool to support decision-making on biosecurity measures that should be prioritized to reduce the probability of introduction of these 2 diseases in dairy herds.

3.
Poult Sci ; 97(9): 3149-3155, 2018 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-29897592

RESUMO

Exogenous enzymatic supplementation of poultry feeds, including α-galactosidase and xylanase, has been shown to increase metabolically available energy, although little information has been published on the impact on amino acid digestibility. An experiment was conducted to investigate a multicarbohydrase containing α-galactosidase and xylanase on amino acid digestibility, ileal digestible energy (IDE), and CP in male broiler chicks. The experiment was a 2 × 2 (diet × enzyme) factorial arrangement with 15 replicates of 8 male broilers per replicate raised for 21 d in a battery setting. The 2 dietary treatments included a positive control (PC) and a negative control (NC) diet formulated to contain 2.5% less calculated AME and digestible amino acids. Each of these diets was fed with and without enzyme. Broilers were fed a starter diet from 0-14 d (crumble) and a grower from 14-21 d (pellet). Birds were sampled on day 21 to determine ileal amino acid digestibility, IDE, and CP digestibility. Titanium dioxide (TiO2) was used as an indigestible marker for the determination of digestibility coefficients. Total ileal amino acid digestibility was increased (P = 0.008) by 3.80% with the inclusion of enzyme. Methionine and lysine digestibility was improved (P < 0.05) with the inclusion of enzyme by 3.37% and 2.61%, respectively. Enzyme inclusion increased (P = 0.001) cysteine digestibility by 9.3%. Diet-influenced ileal amino acid digestibility with tryptophan, threonine, isoleucine, and valine digestibility being increased (P < 0.05) in the PC when compared to the NC. IDE was decreased (P = 0.037) in broilers fed the NC diet by 100 kcal/kg feed when compared to broilers fed the PC diet. Enzyme inclusion increased (P = 0.047) IDE value by 90 kcal/kg. Crude protein digestibility was not influenced by diet; however, similar improvements in CP digestibility with enzyme inclusion were observed as with energy. These data support the benefits of a multicarbohydrase containing α-galactosidase and xylanase inclusion to improve nutrient and ileal amino acid digestibility across multiple dietary nutrient profiles.


Assuntos
Galinhas/fisiologia , Digestão/fisiologia , Endo-1,4-beta-Xilanases/metabolismo , Íleo/fisiologia , alfa-Galactosidase/metabolismo , Aminoácidos/metabolismo , Ração Animal/análise , Fenômenos Fisiológicos da Nutrição Animal/efeitos dos fármacos , Animais , Dieta/veterinária , Proteínas na Dieta/metabolismo , Suplementos Nutricionais/análise , Digestão/efeitos dos fármacos , Endo-1,4-beta-Xilanases/administração & dosagem , Metabolismo Energético/efeitos dos fármacos , Íleo/efeitos dos fármacos , Masculino , Distribuição Aleatória , alfa-Galactosidase/administração & dosagem
4.
Nanoscale ; 10(2): 765-772, 2018 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-29256567

RESUMO

An innovative approach has been developed to measure small molecule diffusion in polyelectrolyte multilayers (PEMs) assembled on colloidal particles by means of flow cytometry (FACS). FACS allows changes in fluorescence emission as a function of time to be recorded per particle in a colloidal dispersion. Dithionite, S2O42-, diffusion in PEMs composed of polyallylamine hydrochloride (PAH) and poly styrene sulfonate (PSS) assembled on silica particles has been studied by recording the quenching of (7-nitrobenz-2-oxa-1,3-diazol-4yl)amino (NBD) labelled PAH layers by FACS. NBD is reduced when it encounters dithionite, and is therefore no longer fluorescent. The decay in fluorescence will be used to follow the kinetics of dithionite diffusion. The fluorescence decay curves show slow diffusion that does not follow classical Fickean law. However, by assuming that the diffusion coefficient is time dependent and follows an inverse power law in an atypical diffusion case, it was possible to obtain an excellent fit for the decay curves.

5.
Nanoscale ; 9(38): 14730-14739, 2017 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-28948261

RESUMO

In the biomedical applications of nanoparticles (NPs), the proper choice of surface chemistry is a crucial aspect in their design. The nature of the coating can heavily impact the interaction of NPs with biomolecules, affect the state of aggregation, and ultimately determine their biological fate. As such, protein corona formation and the aggregation behaviour of gold NPs (Au NPs) are studied here. Au NPs are prepared with four distinct surface functionalisations, namely mercaptosuccinic acid (MSA), N-4-thiobutyroil glucosamine, HS-PEG5000 and HS-alkyl-PEG600. Corona formation, aggregation, and the intracellular behaviour of the Au NPs are then investigated by means of Fluorescence Correlation Spectroscopy (FCS) in cell culture media and in live cells. To evaluate the state of aggregation and the formation of a protein corona, the Au NPs are incubated in cell media and the diffusion coefficient is determined via FCS. The in vitro behaviour is compared with the level of aggregation of the NPs in cells. Diffusion times of the NPs are estimated at different positions in the cell after a one hour incubation period. It is found that the majority of MSA and glucose-Au NPs are present inside the cell as slowly diffusing species with diffusion times (τD) greater than 6000 µs (hydrodynamic diameter >250 nm). PEGylated Au NPs adsorb a small amount of protein and manifest low agglomeration both in media and in living cells. In particular, the HS-alkyl-PEG600 coating shows an excellent correlation between lower protein adsorption, 4-fold lower compared to the MSA coated NPs, and limited intracellular aggregation. In the case of single HS-alkyl-PEG600 coated NPs, it is found that typical intracellular τD values range from 500 to 1500 µs, indicating that these particles display reduced aggregation in the intracellular environment.


Assuntos
Ouro , Nanopartículas Metálicas , Coroa de Proteína , Espectrometria de Fluorescência , Células A549 , Adsorção , Humanos
6.
Acta Trop ; 172: 122-124, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28476601

RESUMO

In Puerto Iguazú City, Argentina, human and canine Visceral Leishmaniasis cases have been recorded since the year 2010, with Leishmania infantum as the etiological agent and Lutzomyia longipalpis as its main vector. In the present study, polymerase chain reaction and sequencing were used to detect L. infantum DNA in 3.9% of the female sandflies captured in Puerto Iguazú City. This is the first report of L. infantum DNA detection in Micropygomyia quinquefer, and the second one in Lu. longipalpis and Nyssomyia whitmani for Argentina. Although the detection of Leishmania DNA itself is not enough to determine a Phlebotomine species as a vector, these results are significant in setting the direction of further investigations of vectorial competence and capacity, necessary to define the roles of different sandflies species as specific or permissive vectors in the transmission VL cycle.


Assuntos
DNA de Protozoário/genética , DNA de Protozoário/isolamento & purificação , Leishmania infantum/genética , Psychodidae/parasitologia , Animais , Argentina/epidemiologia , Cidades , Feminino , Humanos , Insetos Vetores , Leishmaniose Visceral/transmissão , Reação em Cadeia da Polimerase
7.
Horiz. méd. (Impresa) ; 15(4): 52-58, Oct.-Dic.2015. tab, ilus
Artigo em Espanhol | LILACS | ID: lil-786511

RESUMO

Determinar los parámetros bioquímicos enzimáticos (Transaminasa Glutámico Pirúvica (ALT), Transaminasa Glutámico Oxalacetico (AST), Fosfatasa Alcalina (ALP), Gamma Glutamiltransferasa (y-GT), y Lactato Deshidrogenasa (LDH)) en niños con Leucemia Linfoblástica Aguda (LLA) antes del tratamiento antineoplásico.Material y Métodos: Estudio prospectivo experimental, observacional, de corte transversal, en 30 niños con LLA entre 2 y 15 años, en diversos Centros Neoplásicos de Lima. La colecta sanguínea se realizó en tubos BD Vacutainer® de tapa color rojo, procesados en el analizador semiautomatizado BIOTEC® EMP-168, con reactivos enzimáticos de Wiener Lab Group bajo el método modificado de Szaaz y UV-Optimizado por IFCC, SSCC y SFBC. Por último, se realizó la codificación y tabulación de datos.Resultados: El 60% correspondió a niños y el 46,7% corresponde a las edades entre 2 a 6 años. Los niveles séricos de AST estuvieron incrementados en el 33,3% en niños y el 50% en niñas. Los valores séricos de ALT estuvieron incrementados en el 33,3% de niños y en el 41,7% de niñas; solamente el 25% de niñas presentaron valores incrementados de y-GT; la ALP estuvo acrecentada en el 44,4% de niños y en el 66,7% de niñas. Por otro lado los niveles de LDH estuvieron acrecentados en el 55,6% de niños y en el 41,7% de niñas. Conclusión: Las pruebas enzimáticas LDH, AST, ALT y ALP se encuentran aumentados en niños con LLA con respecto a los valores normales, debido al síndrome de lisis tumoral caracterizada por alteraciones electrolíticas, y como consecuencia de la destrucción masiva de células tumorales y la liberación rápida de grandes cantidades de elementos intracelulares...


Objective: To determine the enzymatic biochemical parameters (glutamic pyruvic transaminase (ALT), glutamic oxaloacetic transaminase (AST), alkaline phosphatase (ALP), gamma glutamyltransferase (y-GT), and lactate dehydrogenase (LDH)) in children with acute lymphoblastic leukemia (ALL) before cancer treatment. Material and Methods: A prospective experimental, observational, cross-sectional study was conducted in 30 children between 2 and 15 years old, from several Neoplastic Centers in Lima. Blood collection was perform. in BD r. cap Vacutainer tubes, processed in the semi-automat. analyzer BIOTECO EMP-168, with Wiener Lab Group enzyme reagents under the modified method Szaaz and UV-Optimized by IFCC, SSCC and SFBC. Finally, coding and tabulation was perform.. Results: 60% were boys and 46.7% are between the ages of 2-6 years. Serum levels of AST were increased by 33.3% in boys and 50% in girls. Serum ALT values were increased in 33.3% of boys and 41.7% of girls; only 25% of girls showed increased levels of y-GT values; ALP was increased in 44.4% of boys and 66.7% of girls. Moreover LDH levels were increased in 55.6% of boys and 41.7% of girls. Conclusion: The enzymatic tests LDH, AST, ALT and ALP are increased in children with ALL compared to normal values due to tumor lysis syndrome characterized by electrolyte abnormalities, and as a result of the massive destruction of tumor cells and rapid release of large amounts of intracellular elements...


Assuntos
Humanos , Criança , Alanina Transaminase , Fosfatase Alcalina , Aspartato Aminotransferases , gama-Glutamiltransferase , L-Lactato Desidrogenase , Leucemia-Linfoma Linfoblástico de Células Precursoras , Estudos Transversais , Estudos Observacionais como Assunto , Estudos Prospectivos
8.
Phys Chem Chem Phys ; 18(47): 32396-32405, 2016 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-27869259

RESUMO

The influence of the surface chemistry of polyelectrolyte multilayers (PEMs) on the formation of lipid bilayers is studied here for PEMs with either polyallylamine hydrochloride (PAH) or polydiallyldimethylammonium chloride (PDADMAC) as a polycation as a top layer, and polystyrene sulfonate (PSS) as a polyanion. Small unilamellar vesicles (SUVs) composed of phosphatidyl choline and phosphatidyl serine at a 50 : 50 molar ratio are deposited on top of the PEM films. The assembly of the SUVs into bilayers is studied via a quartz crystal microbalance with dissipation (QCM-D) and fluorescence recovery after photobleaching (FRAP). SUV deposition on PDADMAC/PSS results in vesicle adsorption while on PAH/PSS under the same conditions a bilayer is formed mainly due to weak interactions between the quaternary amines of PDADMAC. FRAP measurements confirm that SUVs are not fused on top of PDADMAC/PSS. The effect of phosphate ions, in solution, on the formation of lipid bilayers is also analysed. X-ray photoelectron spectroscopy shows the complexation of phosphate salts to the primary amines of PAH and no interaction with the quaternary amines of PDADMAC. ζ-potential measurements show a potential close to 0 for the PAH/PSS multilayers in PBS while PDADMAC/PSS displays a potential of 25 mV. A model is presented for the formation of lipid bilayers on PAH/PSS PEMs taking into account the role of phosphate ions in decreasing the electrostatic interactions between SUVs and PEMs and the formation of hydrogen bonds between the phospholipids and the primary amines of PAH.

9.
J Nanosci Nanotechnol ; 16(6): 5696-700, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27427617

RESUMO

In this manuscript we review work of our group on the assembly of lipid layers on top of polyelectrolyte multilayers (PEMs). The assembly of lipid layers with zwitterionic and charged lipids on PEMs is studied as a function of lipid and polyelectrolyte composition by the Quartz Crystal Microbalance. Polyelectrolyte lipid interactions are studied by means of Atomic Force Spectroscopy. We also show the coating of lipid layers for engineering different nanomaterials, i.e., carbon nanotubes and poly(lactic-co-glycolic) nanoparticles and how these can be used to decrease in vitro toxicity and to direct the intracellular localization of nanomaterials.


Assuntos
Engenharia , Ácido Láctico/química , Bicamadas Lipídicas/química , Nanopartículas/química , Nanotecnologia , Nanotubos de Carbono/química , Ácido Poliglicólico/química , Transporte Biológico , Células Hep G2 , Humanos , Espaço Intracelular/metabolismo , Bicamadas Lipídicas/metabolismo , Modelos Moleculares , Conformação Molecular , Copolímero de Ácido Poliláctico e Ácido Poliglicólico , Técnicas de Microbalança de Cristal de Quartzo , Propriedades de Superfície
10.
Nanoscale ; 8(15): 7933-41, 2016 Apr 21.
Artigo em Inglês | MEDLINE | ID: mdl-27006101

RESUMO

Immunostimulating reconstituted influenza virosomes (IRIVs) are liposomes with functional viral envelope glycoproteins: influenza virus hemagglutinin (HA) and neuraminidase intercalated in the phospholipid bilayer. Here we address the fusion of IRIVs to artificial supported lipid membranes assembled on polyelectrolyte multilayers on both colloidal particles and planar substrates. The R18 assay is used to prove the IRIV fusion in dependence of pH, temperature and HA concentration. IRIVs display a pH-dependent fusion mechanism, fusing at low pH in analogy to the influenza virus. The pH dependence is confirmed by the Quartz Crystal Microbalance technique. Atomic Force Microscopy imaging shows that at low pH virosomes are integrated in the supported membrane displaying flattened features and a reduced vertical thickness. Virosome fusion offers a new strategy for transferring biological functions on artificial supported membranes with potential applications in targeted delivery and sensing.


Assuntos
Virossomos/química , Bioengenharia , Coloides , Glicoproteínas de Hemaglutininação de Vírus da Influenza/química , Humanos , Concentração de Íons de Hidrogênio , Vírus da Influenza A Subtipo H1N1/química , Fusão de Membrana , Lipídeos de Membrana/química , Membranas Artificiais , Microscopia de Força Atômica , Nanopartículas/química , Nanopartículas/ultraestrutura , Técnicas de Microbalança de Cristal de Quartzo , Virossomos/ultraestrutura
11.
Chem Commun (Camb) ; 51(77): 14501-4, 2015 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-26280034

RESUMO

Novel mechanized silica nanoparticles incorporating a peptide-based molecular shuttle as a photo-responsive interlocked gatekeeper of nanocontainers are described including the uptake and delivery studies of a model cargo.

12.
Langmuir ; 31(31): 8623-32, 2015 Aug 11.
Artigo em Inglês | MEDLINE | ID: mdl-26158307

RESUMO

The self-assembly of mixed vesicles of zwitterionic phosphatidylcholine (PC) and anionic phosphatidylserine (PS) phospholipids on top of polyelectrolyte multilayers (PEMs) of poly(allylamine hydrochloride) (PAH), as a polycation, and polystyrenesulfonate (PSS), as a polyanion, is investigated as a function of the vesicle composition by means of the quartz crystal microbalance with dissipation (QCM-D), cryo-transmission electron microscopy (Cryo-TEM), atomic force microscopy (AFM), and atomic force spectroscopy (AFS). Vesicles with molar percentages of PS between 50% and 70% result in the formation of lipid bilayers on top of the PEMs. Vesicles with over 50% of PC or over 80% of PS do not assembly into bilayers. AFS studies performed with a PAH-modified cantilever approaching and retracting from the lipid assemblies reveal that the main interaction between PAH and the lipids takes place through hydrogen bonding between the amine groups of PAH and the carboxylate and phosphate groups of PS and with the phosphate groups of PC. The interaction of PAH with PS is much stronger than with PC. AFS measurements on assemblies with 50% PC and 50% PS revealed similar adhesion forces to pure PS assemblies, but the PAH chains can reorganize much better on the lipids as a consequence of the presence of PC. QCM-D experiments show that vesicles with a lipid composition of 50% PC and 50% PS do not form bilayers if PSS is replaced by alginate (Alg) or poly(acrylic acid) (PAA).


Assuntos
Bicamadas Lipídicas/química , Poliaminas/química , Poliestirenos/síntese química , Eletrólitos/química , Ligação de Hidrogênio , Tamanho da Partícula , Poliestirenos/química
13.
J Neural Eng ; 12(4): 046023, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-26083683

RESUMO

OBJECTIVE: Visual P300-based brain-computer interface spellers offer a useful communication channel for locked-in patients, who are completely dependent in their daily lives. One of the research goals for these systems is to achieve greater communication rates by means of modifying some features of their interfaces, e.g., reducing the matrix size. However, such modifications may not work well with disabled end-users, such as patients of amyotrophic lateral sclerosis (ALS), due to a supposed reduction of their cognitive resources. The purpose of the present study was to provide a proof of concept that ALS patients could efficiently use a P300-based speller with a 4 × 3 symbol matrix based on the T9 interface developed for mobile phones. APPROACH: We conducted an experiment with a sample of 11 able-bodied participants and one locked-in patient with ALS. All participants tested our T9-like visual P300-based speller and also two different 7 × 6 matrix spellers based on Farwell and Donchin's classic proposal-one of them included a word predictor system like the T9-like speller did. MAIN RESULTS: The performance analyses indicated that the locked-in patient benefited from using a reduced matrix size as much as healthy users did, spelling words almost 1.6 times faster and equally accurately when using the T9-like speller than when using the alternative spellers. SIGNIFICANCE: Due to counting on only one locked-in patient, the current work constitutes a feasibility study. The actual usability of systems such as the one proposed in this paper should be determined by means of studies with a greater number of end-users in real-life conditions.


Assuntos
Esclerose Amiotrófica Lateral/fisiopatologia , Interfaces Cérebro-Computador , Auxiliares de Comunicação para Pessoas com Deficiência , Eletroencefalografia/métodos , Potencial Evocado P300 , Percepção Visual , Adulto , Algoritmos , Esclerose Amiotrófica Lateral/reabilitação , Mapeamento Encefálico/métodos , Feminino , Humanos , Idioma , Masculino , Projetos Piloto , Desempenho Psicomotor , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Processamento de Texto/métodos , Adulto Jovem
14.
Phys Chem Chem Phys ; 17(12): 8239-47, 2015 Mar 28.
Artigo em Inglês | MEDLINE | ID: mdl-25732124

RESUMO

Linking amino and hydroxycoumarins to BODIPYs through the amino or hydroxyl group lets the easy construction of unprecedented photostable coumarin-BODIPY hybrids with broadened and enhanced absorption in the UV spectral region, and outstanding wavelength-tunable laser action within the green-to-red spectral region (∼520-680 nm). These laser dyes allow the generation of a valuable tunable UV (∼260-350 nm) laser source by frequency doubling, which is essential to study accurately the photochemistry of biological molecules under solar irradiation. The tunability is achieved by selecting the substitution pattern of the hybrid. Key factors are the linking heteroatom (nitrogen vs. oxygen), the number of coumarin units joined to the BODIPY framework and the involved linking positions.


Assuntos
Compostos de Boro/química , Cumarínicos/química , Raios Ultravioleta , Técnicas Eletroquímicas , Transferência Ressonante de Energia de Fluorescência , Corantes Fluorescentes/química
15.
Horiz. méd. (Impresa) ; 14(4): 6-14, oct.-dic. 2014. ilus, tab
Artigo em Espanhol | LILACS | ID: lil-732072

RESUMO

Determinar la seroprevalencia de marcadores infecciosos causantes de pérdidas de hemodonaciones en el Servicio de Banco de Sangre del Hospital Nacional Docente Madre-Niño San Bartolomé de enero 2008 a diciembre del 2013. Material y Métodos: Estudio retrospectivo, de corte transversal, descriptivo. El criterio de inclusión fue hemodonaciones completas sin complicaciones que cumplían con los criterios de calidad y normatividad del PRONAHEBAS. El análisis de datos se realizó en tres procesos básicos: codificación, tabulación y construcción de tablas y gráficos. La técnica utilizada para la verificación estadística de los resultados fue mediante el analizador estadístico SPSS versión 20.0. Resultados: Los hallazgos fueron: 4.63% para HBcAb, 1.78% para sífilis, 1.21% para HTLV I-II, y 5.31% para otros marcadores serológicos de un total de 11399 donaciones completas. La prevalencia general fue de 9.36% para todos los marcadores, lo cual ocasiono una pérdida de 1016 donaciones; 457.2 Litros de sangre y 61,893.28 USD perdidos. Las asociaciones entre marcadores infecciosos más frecuentes fueron: HBcAb con sífilis y HBsAg y los tres componentes que explican la varianza fueron asociados por cronicidad y epidemias concentradas en poblaciones, por exposición ocupacional y por relación subrogante. Conclusión: La prevalencia hallada demostró la mala calidad de donantes de sangre y el gran impacto económico por hemoderivados desechados muestran las limitaciones en la cadena de donación. Por lo que es conveniente continuar con las campañas de educación sanitaria, las buenas prácticas en medicina transfusional y la selección de donantes de sangre para prevenir las infecciones transmisibles por transfusión, aumentar el suministro de sangre sin poner en riesgo al receptor y sin nuevas donaciones, asimismo reducir el costo económico perdido por donación...


To determine the seroprevalence of infectious markers causing loss of blood donations in the Blood Bank Service in Hospital Nacional Docente Madre Niño San Bartolome January 2008 to December 2013. Material and Methods: A retrospective, cross-sectional, descriptive study was conducted. Inclusion criteria were donations of whole blood without complications that meet the quality criteria and standards of PRONAHEBAS. Data analysis was conducted in three basic processes: coding, tabulation and construction of tables and graphs. The technique used for the statistical verification of the results was by SPSS version 20.0 statistical analyzer. Results: The findings were 4.63% for HBcAb, 1.78% for syphilis, 1.21% for antibodies to HTLV I-II, and 5.31% for other serological markers of a total of 11399 complete donations. The overall prevalence was 9.36% for all markers which caused a loss of 1016 donations, 457.2 liters of blood, and 61893.28 USD lost. Similarly, frequent associations between infectious markers were HBcAb that of syphilis and HBsAg, and the three components explaining the variance were associated with chronicity and concentrated epidemics in populations, occupational exposure and surrogate relationship. Conclusion: The prevalence found shows the poor quality of blood donors and the high economic impact of discarded blood show the limitations of the donation chain. So it is appropriate to continue sanitary education campaigns, best practices in transfusion medicine and selection of blood donors to prevent transfusion-transmissible infections, increase the blood supply without compromising the receiver without new donations also reduce the economic cost lost by donation...


Assuntos
Humanos , Bancos de Sangue , Peneiramento de Líquidos , Estudos Transversais , Epidemiologia Descritiva , Peru
16.
Actas Dermosifiliogr ; 105(10): 923-34, 2014 Dec.
Artigo em Inglês, Espanhol | MEDLINE | ID: mdl-24852726

RESUMO

Psoriatic lesions affecting the scalp, nails, palms, and the soles of the feet are described as difficult-to-treat psoriasis and require specific management. Involvement of these sites often has a significant physical and emotional impact on the patient and the lesions are difficult to control with topical treatments owing to inadequate penetration of active ingredients and the poor cosmetic characteristics of the vehicles used. Consequently, when difficult-to-treat sites are involved, psoriasis can be considered severe even though the lesions are not extensive. Scant information is available about the use of biologic therapy in this setting, and published data generally comes from clinical trials of patients who also had moderate to severe extensive lesions or from small case series and isolated case reports. In this article we review the quality of the scientific evidence for the 4 biologic agents currently available in Spain (infliximab, etanercept, adalimumab, and ustekinumab) and report level i evidence for the use of biologics to treat nail psoriasis (level of recommendation A) and a somewhat lower level of evidence in the case of scalp involvement and palmoplantar psoriasis.


Assuntos
Fatores Biológicos/uso terapêutico , Dermatoses do Pé/tratamento farmacológico , Dermatoses da Mão/tratamento farmacológico , Doenças da Unha/tratamento farmacológico , Psoríase/tratamento farmacológico , Dermatoses do Couro Cabeludo/tratamento farmacológico , Terapia Biológica , Medicina Baseada em Evidências , Humanos
17.
Fisioterapia (Madr., Ed. impr.) ; 35(4): 154-166, jul.-ago. 2013. ilus, tab
Artigo em Espanhol | IBECS | ID: ibc-114578

RESUMO

Objetivo: Estimar la reproducibilidad interevaluador del Formato de Observación Sistemáticade la Alineación Corporal (FOSAC), aplicado a adultos jóvenes de una universidad privada deBucaramanga (Colombia).Metodología: Se realizó un estudio de pruebas diagnósticas en una muestra de 123 estudiantesuniversitarios de 18 a 29 a˜nos de edad, que cumplieran con los criterios de inclusión establecidos.Tres examinadores (un experto en postura, una fisioterapeuta con experiencia y unaestudiante de último semestre de fisioterapia) realizaron el examen postural, analizando 7 fotografíasimpresas: una posterior, 2 laterales derechas, 2 laterales izquierdas y 2 anteriores. Seexaminaron 63 deficiencias que fueron registradas en el FOSAC.Resultados: La reproducibilidad interevaluador para determinar deficiencias posturales oscilóentre pobre y aceptable. Los planos que obtuvieron mejor reproducibilidad de acuerdo alíndice kappa global fueron el lateral izquierdo y derecho, en los cuales el 35,7% de las deficienciasobtuvo un kappa por encima de 0,40. Escoliosis en S fue la deficiencia con mayorreproducibilidad en el ítem experto vs. estudiante en el plano posterior, con un valor kappa de1,00.Conclusiones: La baja reproducibilidad interevaluador conduce a considerar que la exploraciónpostural por observación directa no es el método adecuado para usar en investigaciones. Sinembargo, permite al profesional detectar deficiencias posturales marcadas; por esta razón,no debería ser excluida del proceso de evaluación fisioterapéutica. Por otra parte, el FOSACpuede constituirse en una herramienta útil en la formación académica del futuro profesional enfisioterapia, al establecer metódicamente el proceso de observación de la alineación corporal (AU)


Objective: To estimate the inter-rater reliability of the Body Alignment Systematic ObservationForm (BASOF) applied to young adults in a private university in Bucaramanga (Colombia).Methodology: A study of diagnostic tests was performed in a sample of 123 college students,aged 18 to 29, who met the inclusion criteria. Three examiners (an expert in posture, a physiotherapistwith experience and a last semester student of physiotherapy) performed the posturalexamination, analyzing 7 printed photographs: one posterior, two right laterals, two left lateralsand two anterior. We studied 63 deficiencies, which were registered in the BASOF.Results: The inter-rater reliability to determine postural deficiencies ranged from poor toacceptable. The level obtaining the best reliability according to the global Kappa index wasthe posterior one in which 26.5% of the deficiencies obtained a kappa Index greater than0.40. The scoliosis in S variable had the highest reliability with a kappa value of 1.00, obtainedon the item expert vs student on the posterior plane.Conclusions: The low inter-rater reliability leads to the conclusion that postural examination bydirect observation method is not suitable for use in research. However, it allows the practitionerto detect marked postural deficiencies and therefore should not be excluded from the physiotherapyexamination. Moreover, the BASOF can become a useful tool in the future academiccareer in physical therapy to establish methodically the process of observing body alignment (AU)


Assuntos
Humanos , Masculino , Feminino , Adolescente , Adulto Jovem , Adulto , Mau Alinhamento Ósseo/reabilitação , Postura , Estudos Observacionais como Assunto , Reprodutibilidade dos Testes
18.
Semergen ; 39(1): 12-7, 2013.
Artigo em Espanhol | MEDLINE | ID: mdl-23517892

RESUMO

INTRODUCTION: Minor Surgery is an ever-increasing programmed activity in Primary Health Care Centres. The aim of this paper is to determine the relationship between the clinical diagnosis prior to Minor Surgery and its concordance with the histological results. MATERIAL AND METHODS: We carried out a retrospective study using the registered activity log in a Primary Health Care Centre in the region of Valdejalón of Aragón. SUBJECTS: A total of 1231 patients were included between April 1999 and June 2009. A total of 1391 diagnoses were given, together with the corresponding medical care. MEASUREMENTS: Clinical diagnosis and histological results, and the level of correlation between both. RESULTS: A total of 820 samples were submitted for histological study (59% of the total sample). Eleven of the main clinical and histological diagnoses were evaluated, and a contingency table was prepared. The result of the correlation gave a Kappa coefficient of 0.638. CONCLUSIONS: Once the results were analysed and compared to similar studies of Minor Surgery carried out in Primary Care, our results confirm that there is a high correlation between the clinical diagnosis and the histological results.


Assuntos
Diagnóstico , Procedimentos Cirúrgicos Menores/estatística & dados numéricos , Patologia Clínica , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Saúde da População Rural , Espanha , Adulto Jovem
19.
Artigo em Espanhol | IBECS | ID: ibc-109164

RESUMO

Introducción. La cirugía menor forma parte de las actividades programadas en un número cada vez más creciente de nuestros centros de atención primaria. El objetivo de este trabajo es conocer el grado de correlación entre el diagnóstico clínico previo al proceso de cirugía menor y su concordancia con el resultado de anatomía patológica. Material y método. Para ello realizamos un estudio retrospectivo a partir del Registro de Actividades de Cirugía Menor de un Centro de Salud Rural de la Comarca de Valdejalón de Aragón. Sujetos. Se incluyeron 1.231 pacientes atendidos durante el periodo comprendido entre abril de 1999 y julio del año 2009 a los que se realizaron un total de 1.391 diagnósticos con sus correspondientes actuaciones. Mediciones. Diagnóstico clínico y resultado clínico-patológico. Correlaciones entre ambos Resultados Se remitieron 820 piezas para estudio clínico-patológico (el 59% de las intervenidas). Se valoraron los 11 diagnósticos clínicos y clínico-patológicos principales con los que se construyó una tabla de contingencia. Se obtuvo un coeficiente kappa de 0,638 Conclusiones. Analizados estos resultados y comparándolos con otros trabajos similares realizados en el ámbito de la cirugía menor en atención primaria, podemos afirmar que hay una buena correspondencia entre el diagnóstico clínico inicial y los resultados de anatomía patológica (AU)


Introduction. Minor Surgery is an ever-increasing programmed activity in Primary Health Care Centres. The aim of this paper is to determine the relationship between the clinical diagnosis prior to Minor Surgery and its concordance with the histological results. Material and methods. We carried out a retrospective study using the registered activity log in a Primary Health Care Centre in the region of Valdejalón of Aragón. Subjects. A total of 1231 patients were included between April 1999 and June 2009. A total of 1391 diagnoses were given, together with the corresponding medical care. Measurements. Clinical diagnosis and histological results, and the level of correlation between both. Results. A total of 820 samples were submitted for histological study (59% of the total sample). Eleven of the main clinical and histological diagnoses were evaluated, and a contingency table was prepared. The result of the correlation gave a Kappa coefficient of 0.638. Conclusions. Once the results were analysed and compared to similar studies of Minor Surgery carried out in Primary Care, our results confirm that there is a high correlation between the clinical diagnosis and the histological results (AU)


Assuntos
Humanos , Masculino , Feminino , Adulto , Procedimentos Cirúrgicos Menores/instrumentação , Procedimentos Cirúrgicos Menores/métodos , Procedimentos Cirúrgicos Menores , Atenção Primária à Saúde/métodos , Atenção Primária à Saúde/tendências , Atenção Primária à Saúde , Procedimentos Cirúrgicos Menores/estatística & dados numéricos , Procedimentos Cirúrgicos Menores/normas , Procedimentos Cirúrgicos Menores/tendências , Estatísticas não Paramétricas , Atenção Primária à Saúde/organização & administração , Atenção Primária à Saúde/normas , Estudos Retrospectivos , Cuidados Pós-Operatórios/métodos , Cuidados Pós-Operatórios/estatística & dados numéricos
20.
J Nanosci Nanotechnol ; 12(6): 4836-42, 2012 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-22905538

RESUMO

Carbon Nanotubes have been functionalized with a layer of poly (sulfopropyl methacrylate) synthesized from silane initiators attached to the walls of the Carbon nanotubes. On top of the poly sulfo propyl methacrylate, lipid vesicles composed of 75% 1,2-Dioleoyl-sn-Glycero-3-Phosphocholine and 25% 1,2-Dioleoyl-sn-Glycero-3-[Phospho-L-Serine] were assembled. The surface modification of the Carbon Nanotubes and lipid assembly were followed by TEM. Confocal Raman Microscopy was used to study the uptake and localization of the surface modified Carbon Nanotubes in the HepG2 cell line. The localization of the Carbon Nanotubes in the cells was affected by the surface coating. It was found that poly (sulfopropyl methacrylate) and lipid modified Carbon Nanotubes were present in the region of the lipid bodies in the cytoplasm.


Assuntos
Materiais Revestidos Biocompatíveis/química , Cristalização/métodos , Lipídeos/química , Nanotubos de Carbono/química , Frações Subcelulares/química , Eletrólitos/química , Células Hep G2 , Humanos , Substâncias Macromoleculares/química , Teste de Materiais , Conformação Molecular , Tamanho da Partícula , Propriedades de Superfície , Distribuição Tecidual
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