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1.
Appl Microbiol Biotechnol ; 104(24): 10541-10553, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33104843

RESUMO

Agar-degrading bacteria are crucial drivers for the carbon cycle in the marine environments due to their ability that use algae as a carbon source. Although numerous agar-degrading bacteria and agarases have been reported, little is known about expression levels of agar-degrading genes in wild strains. Here, the genome of an agar-hydrolyzing marine bacterium, Catenovulum maritimus Q1T, was sequenced and annotated with 11 agarase and 2 neoagarooligosaccharide hydrolase genes. Quantitative PCR revealed that all the annotated agar-degrading genes were expressed consistently that initially upregulated and then gradually downregulated under agarose induction. Moreover, the presence of glucose inhibited the agar-degrading ability, in terms of both gene expression and enzymatic activity. These facts indicated the agar-degrading ability of wild bacteria was mainly induced by agarose and repressed by the available carbon source. Additionally, a ß-agarase, AgaQ1, belonging to the GH16 family, with high expression in strain Q1T, was cloned and characterized. Biochemical analysis showed that the recombinant AgaQ1 was substrate-specific, yielding neoagarotetraose and neoagarohexaose as the main products. It exhibited optimal activity at 40 °C, pH 8.0, and an agarose concentration of 1.6% (w/v). Besides, AgaQ1 showed a high-specific activity (757.7 U/mg) and stable enzymatic activity under different ion or agent treatments; thus, AgaQ1 has great potential in industrial applications. KEY POINTS: • The genome of C. maritimus Q1T was sequenced and annotated with 11 agarases and 2 Nabh genes. • The expression of agar-degrading genes in the strain C. maritimus Q1T was induced by agarose. • Glucose was the carbon source utilized prior to agarose for bacterial growth. • A ß-agarase, AgaQ1, with high expression and activity was identified.

2.
Microbiome ; 8(1): 126, 2020 08 31.
Artigo em Inglês | MEDLINE | ID: mdl-32867860

RESUMO

BACKGROUND: Bacterial predation is an important selective force in microbial community structure and dynamics. However, only a limited number of predatory bacteria have been reported, and their predatory strategies and evolutionary adaptations remain elusive. We recently isolated a novel group of bacterial predators, Bradymonabacteria, representative of the novel order Bradymonadales in δ-Proteobacteria. Compared with those of other bacterial predators (e.g., Myxococcales and Bdellovibrionales), the predatory and living strategies of Bradymonadales are still largely unknown. RESULTS: Based on individual coculture of Bradymonabacteria with 281 prey bacteria, Bradymonabacteria preyed on diverse bacteria but had a high preference for Bacteroidetes. Genomic analysis of 13 recently sequenced Bradymonabacteria indicated that these bacteria had conspicuous metabolic deficiencies, but they could synthesize many polymers, such as polyphosphate and polyhydroxyalkanoates. Dual transcriptome analysis of cocultures of Bradymonabacteria and prey suggested a potential contact-dependent predation mechanism. Comparative genomic analysis with 24 other bacterial predators indicated that Bradymonabacteria had different predatory and living strategies. Furthermore, we identified Bradymonadales from 1552 publicly available 16S rRNA amplicon sequencing samples, indicating that Bradymonadales was widely distributed and highly abundant in saline environments. Phylogenetic analysis showed that there may be six subgroups in this order; each subgroup occupied a different habitat. CONCLUSIONS: Bradymonabacteria have unique living strategies that are transitional between the "obligate" and the so-called facultative predators. Thus, we propose a framework to categorize the current bacterial predators into 3 groups: (i) obligate predators (completely prey-dependent), (ii) facultative predators (facultatively prey-dependent), and (iii) opportunistic predators (prey-independent). Our findings provide an ecological and evolutionary framework for Bradymonadales and highlight their potential ecological roles in saline environments. Video abstract.

3.
Int J Syst Evol Microbiol ; 70(10): 5473-5478, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32886590

RESUMO

A Gram-stain-negative, non-motile, coccus-shaped, catalase- and oxidase-positive, facultatively anaerobic and pink-pigmented bacterium, designated strain CQN31T, was isolated from sediment of Changqiaohai Lake, Yunnan Province, China. Growth occurred at 4-45 °C (optimum, 37 °C), at pH 6.5-9.5 (optimum, pH 8.0) and with 0-1 % (w/v) NaCl (optimum, 0 %). C18 : 1 ω7c/C18 : 1 ω6c and C16 : 0 were the predominant fatty acids. Phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), phosphatidyldimethylethanolamine (PME) and one unidentified aminolipid (AL) were the major polar lipids. The G+C content of the genomic DNA was 71.5 %. 16S rRNA gene sequence comparisons indicated that strain CQN31T shared 96.8 % similarity with Roseomonas wooponensis JCM 19527T and 95.9 % with R. terricola EM0302T. Digital DNA-DNA hybridization values between strain CQN31T and Roseomonas stagni DSM 19981T, R. rosea DSM 14916T and R. mucosa NCTC 13291T were 21.0, 19.4 and 19.8 %, respectively. Average amino acid identity and average nucleotide identity values between strain CQN31T and R. stagni DSM 19981T, R. rosea DSM 14916T and R. mucosa NCTC 13291T were 73.7, 63.4 and 61.9 %, and 79.2, 77.1 and 77.5%, respectively. Distinct morphological, physiological and genotypic differences from previously described taxa support the classification of strain CQN31T as a representative of a novel species in the genus Roseomonas, for which the name Roseomonas bella sp. nov. is proposed. The type strain is CQN31T (=KCTC 62447T=MCCC 1H00309T).


Assuntos
Sedimentos Geológicos/microbiologia , Lagos/microbiologia , Methylobacteriaceae/classificação , Filogenia , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Methylobacteriaceae/isolamento & purificação , Hibridização de Ácido Nucleico , Fosfolipídeos/química , Pigmentação , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
4.
Int J Syst Evol Microbiol ; 70(9): 5001-5011, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32783807

RESUMO

Kiritimatiellaeota is widespread and ecologically important in various anoxic environments. However, the portion of culturable bacteria within this phylum is quite low and, in fact, there is only one currently described species. In this study, a novel anaerobic, non-motile, coccoid, Gram-stain-negative bacterial strain, designated S-5007T, was isolated from surface marine sediment. The 16S rRNA gene sequence was found to have very low 16S rRNA gene sequence similarity to the nearest known type strain, Kiritimatiella glycovorans L21-Fru-ABT (84.9 %). The taxonomic position of the novel isolate was investigated using a polyphasic approach and comparative genomic analysis. Phylogenetic analyses based on 16S rRNA genes and genomes indicated that strain S-5007T branched within the radiation of the phylum Kiritimatiellaeota. Different from the type strain, strain S-5007T can grow under microaerobic conditions, and the genomes of strain S-5007T and the other strains in its branch have many more antioxidant-related genes. Meanwhile, other different metabolic features deduced from genome analysis supported the separate evolution of the proposed class (strain S-5007T branch) and K. glycovorans L21-Fru-ABT. Based on phylogenetic and phenotypic characterization studies, Tichowtungia aerotolerans gen. nov., sp. nov. is proposed with S-5007T (=MCCC 1H00402T=KCTC 15876T) as the type strain, as the first representative of novel taxa, Tichowtungiales ord. nov., Tichowtungiaceae fam. nov. in Tichowtungiia class. nov.


Assuntos
Sedimentos Geológicos/microbiologia , Cocos Anaeróbios Gram-Negativos/classificação , Filogenia , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Cocos Anaeróbios Gram-Negativos/isolamento & purificação , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
5.
Int J Syst Evol Microbiol ; 70(6): 3679-3685, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32416740

RESUMO

A Gram-stain-negative, rod-shaped (0.2-0.3×1.0-2.4 µm), catalase-positive, oxidase-negative and non-motile bacterium, designated strain RZ26T, was isolated from the marine red algae collected from the coast of Weihai, PR China. Growth of strain RZ26T occurred at 15-33 °C (optimum, 25-28 °C), pH 6.0-9.5 (optimum, pH 7.0-7.5) and 0.5-5.0 % (w/v) NaCl (optimum, 2.0-3.0 %). Resuls of phylogenetic analysis based on 16S rRNA gene sequences showed that strain RZ26T was most closely related to Maribacter spongiicola DSM 25233T (96.2 % sequence similarity), followed by Maribacter forsetii DSM 18668T (96.1 %) and Maribacter vaceletii DSM 25230T (95.4 %). The average nucleotide identity and the average amino acid identity values between strain RZ26T and M. sedimenticola KCTC 12966T, M. spongiicola DSM 25233T, M. vaceletii DSM 25230T and M. forsetii DSM 18668T were 75.6, 76.2, 76.0, 76.7, 64.3, 63.9, 68.6 and 68.0 %, respectively. The digital DNA-DNAhybridization values based on the draft genomes between strain RZ26T and M. sedimenticola KCTC 12966T, M. spongiicola DSM 25233T and M. vaceletii DSM 25230T were 38.0, 35.1 and 37.1 %, respectively. The major fatty acids in strain RZ26T were iso-C17 : 0 3-OH, iso-C15 : 0 and C16 : 1 ω7c/C16 : 1 ω6c. The major respiratory quinone was MK-6. The dominant polar lipid was phosphatidylethanolamine. The DNA G+C content was 38.0 mol%. Phylogenetic analysis shows strain RZ26T fell within a clade comprising species of the genus Maribacter. Polyphasic taxonomy indicates that the isolate represents a novel species of the genus Maribacter, for which the name Maribacter algarum sp. nov. is proposed, with type strain RZ26T (=KCTC 62992T=MCCC 1H00362T).


Assuntos
Flavobacteriaceae/classificação , Filogenia , Rodófitas/microbiologia , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Flavobacteriaceae/isolamento & purificação , Hibridização de Ácido Nucleico , Fosfatidiletanolaminas/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/química
6.
Int J Syst Evol Microbiol ; 70(6): 3588-3596, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32453683

RESUMO

A Gram-stain-negative, non-motile, fine rod or short filament shaped, jacinth pigmented bacterium, designated strain WDS2C27T, was isolated from a marine solar saltern in Wendeng, Weihai, PR China (37°31'5″ N, 122°1'47″ E). Growth of WDS2C27T occurred at 20-42 °C (optimum 37 °C) and pH 6.5-8.5 (optimal pH 7.0-8.0). Optimal growth occurred in modified marine broth containing 6 % (w/v) NaCl. The major polar lipids in WDS2C27T were phosphatidylethanolamine, two unidentified aminolipids and one unidentified lipid. The major respiratory quinone of WDS2C27T was MK-6. The dominant fatty acids were iso-C15 : 0 and anteiso-C15 : 0. The DNA G+C content was 35.0 mol%. The nucleotide sequence of the 16S rRNA gene indicated that the most closely related strain was Psychroflexus planctonicus X15M-8T (92.0 % over 1452 bp). WDS2C27T showed 60.7 % average amino acid identity, 55.6 % percentage of conserved proteins, 75.0 % average nucleotide identity and 13.1 % digital DNA-DNA hybridization identity with the type species of the genus Psychroflexus, Psychroflexus torquis ATCC 700755T. The phenotypic and genotypic properties and phylogenetic inference indicated that WDS2C27T could be assigned to a novel species within a novel genus, for which the name Mesohalobacter halotolerans gen. nov., sp. nov. is proposed. Strain WDS2C27T (=MCCC 1H00133T=KCTC 52044T) is the type strain.


Assuntos
Flavobacteriaceae/classificação , Filogenia , Salinidade , Microbiologia da Água , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Flavobacteriaceae/isolamento & purificação , Hibridização de Ácido Nucleico , Fosfatidiletanolaminas/química , Fosfolipídeos/química , Pigmentação , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/química
7.
Int J Syst Evol Microbiol ; 70(3): 1617-1622, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32228747

RESUMO

A taxonomic study was carried out on strain SH27T, which was isolated from seawater collected around Xiaoshi Island, PR China. Cells of strain SH27T were Gram-stain-negative, non-motile, rod-shaped, orange-pigmented and grew at 15-37 °C (optimum, 28 °C), at pH 6.0-8.0 (pH 7.0) and in 1.0-7.0 % (w/v) NaCl (2.0-3.0 %). The isolate was positive for catalase, but negative for nitrate reduction, oxidase, indole production and urease. Carotenoid pigment was produced. Phylogenetic analysis based on the 16S rRNA gene placed strain SH27T in the genus Dokdonia with the closest relative being Dokdonia donghaensis KCTC 12391T, exhibiting 96.7 % 16S rRNA gene pairwise similarity. The results of genomic comparisons, including average nucleotide identity and digital DNA-DNA hybridization, showed 72.9 and 19.2 % identity to D. donghaensis KCTC 12391T, respectively. The major cellular fatty acids were iso-C15 : 0, iso-C15 : 1 G and iso-C17 : 0 3-OH. The major polar lipids were phosphatidylethanolamine and two unidentified lipids. Menaquinone-6 was the only respiratory quinone. The G+C content of the genomic DNA was 32.9 mol%. On the basis of the phenotypic and phylogenetic data, strain SH27T represents a novel species of the genus Dokdonia, for which the name Dokdonia sinensis sp. nov. is proposed, with the type strain SH27T (MCCC 1H00358T=CCTCC AB 2018323T=KCTC 62962T).


Assuntos
Flavobacterium/classificação , Filogenia , Água do Mar/microbiologia , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Flavobacteriaceae/classificação , Flavobacterium/isolamento & purificação , Hibridização de Ácido Nucleico , Fosfatidiletanolaminas/química , Pigmentação , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/química , Microbiologia da Água
8.
Int J Syst Evol Microbiol ; 70(1): 100-104, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31535968

RESUMO

A Gram-stain-negative, aerobic and curved-rod-shaped bacterium, designated QM202T, was isolated from red algae (Gracilaria blodgettii). Cells of strain QM202T were 0.2-0.3 µm wide and 1.0-2.5 µm long, catalase-negative and oxidase-positive. The strain exhibited an agar-degrading activity. It was motile by means of a single polar flagellum. Optimal growth occurred at 28-30 °C, pH 7.0-7.5 and in the presence of 2.0-3.0 % (w/v) NaCl. The DNA G+C content was 41.4 mol%. The isoprenoid quinone was identified as Q-8. Phophatidylethanolamine and phosphatidylglycerol were the predominant phospholipids. The dominant fatty acids were C18:1ω7c, C16:0 and C16:1ω7c and/or iso-C15:0 2-OH. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain QM202T belonged to the genus Marinomonas. The closest described neighbour in terms of 16S rRNA gene sequence identity was Marinomonas blandensis MED121T (95.5 %). The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness indicated that strain QM202T can be considered to represent a novel species, Marinomonas agarivorans sp. nov. The type strain is QM202T (=KCTC 52475T=MCCC 1H00145T).


Assuntos
Gracilaria/microbiologia , Marinomonas/classificação , Filogenia , Ágar , Alteromonadaceae/classificação , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Marinomonas/isolamento & purificação , Oceanos e Mares , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
9.
Int J Syst Evol Microbiol ; 70(1): 193-198, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31617845

RESUMO

A Gram-stain-negative, non-motile, rod-shaped, oxidase-positive, red-pigmented bacterium, strain N3T, was isolated from Fuxian lake, a freshwater lake in Yunnan Province, PR China. Strain N3T was facultatively anaerobic, heterotrophic and negative for catalase. Optimal growth occurred at 30 °C (range 4-45 °C), pH 7.0-8.0 (range 6.5-9.5) and in the presence of 0-3 % (w/v) NaCl (range 0-3 %). The results of phylogenetic analysis based on 16S rRNA gene sequencing revealed that strain N3T was close to the type strains of Algoriphagus aquaeductus, Algoriphagus shivajiensis and Algoriphagus alkaliphilus with sequence similarities of 97.4, 97.3 and 97.2 % respectively. The G+C content of the genomic DNA was 43.9 mol%. The quinone system contained menaquinone MK-7 as the sole component. The major fatty acids were iso-C15 : 0, summed feature 9 (10-methyl C16 : 0 and/or iso-C17 : 1 ω9c), summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω 7c) and iso-C16 : 0. Major polar lipids were phosphatidylcholine, phosphatidylethanolamine, an unidentified glycolipid, one unidentified phospholipid, two unidentified aminolipids and four unidentified lipids. On the basis of physiological, chemotaxonomic and molecular properties as well as phylogenetic distinctiveness, strain N3T should be placed into the genus Algoriphagus as a novel species, for which the name Algoriphagus lacus sp. nov. is proposed. The type strain is N3T (=KCTC 62622T=MCCC 1H00308T).


Assuntos
Bacteroidetes/classificação , Lagos/microbiologia , Filogenia , Microbiologia da Água , Técnicas de Tipagem Bacteriana , Bacteroidetes/isolamento & purificação , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Fosfolipídeos/química , Pigmentação , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/química
10.
Int J Syst Evol Microbiol ; 70(3): 1555-1561, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31860427

RESUMO

A Gram-stain-positive, facultatively anaerobic bacterium, strain JDX10T, was isolated from a soil sample of Fildes Peninsula, Antarctica. Cells of the strain were irregular rod-shaped and non-motile. Cells grew at 4-40 °C (optimum, 28 °C), at pH 6.0-9.0 (optimum, 7.5) and with 0.0-3.0 % (w/v) NaCl (optimum, 1.0 %). According to phylogenetic analysis based on 16S rRNA gene sequences, strain JDX10T was associated with the genus Tessaracoccus, and showed highest similarities to Tessaracoccus rhinocerotis CCTCC AB 2013217T (97.2 %), Tessaracoccus flavescens SST-39T (96.9 %) and Tessaracoccus terricola JCM 32157T (96.9 %). The average nucleotide identity scores of strain JDX10T to T. rhinocerotis CCTCC AB 2013217T and T. bendigoensis JCM 13525T were 74.8 and 73.3 %, respectively and the Genome-to-Genome Distance Calculator scores were 19.2 and 18.7 %, respectively. The major (>10.0 %) cellular fatty acid was anteiso-C15 : 0. The predominant isoprenoid quinone was MK-10(H4). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and one unidentified glycolipid. The phylogenetic analysis and physiological and biochemical data showed that strain JDX10T should be classified as representing a novel species in the genus Tessaracoccus, for which the name Tessaracoccus antarcticus sp. nov. is proposed. The type strain is JDX10T (=MCCC 1H00351T=KCTC 49242T).


Assuntos
Filogenia , Propionibacteriaceae/classificação , Rodopsina , Microbiologia do Solo , Regiões Antárticas , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Glicolipídeos/química , Fosfolipídeos/química , Propionibacteriaceae/isolamento & purificação , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Ubiquinona/análogos & derivados , Ubiquinona/química
11.
Int J Syst Evol Microbiol ; 70(1): 576-581, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31613746

RESUMO

Strain F3212T, Gram-stain-negative, aerobic, helical and motile bacterium, was isolated from the marine sediment collected in a sea cucumber culture pond located in Rongcheng, China. Strain F3212T grew optimally at pH 8.5, at 30 °C and in the presence of 3.0 % (w/v) NaCl. Phylogenetic analysis, based on 16S rRNA gene sequences, indicated that strain F3212T belongs to the genus Marinospirillum, clustering with M. celere, M. alkaliphilum, M. minutulum, M. megaterium and M. insulare (with 96.4, 94.6, 93.1, 92.4 and 92.1 % 16S rRNA gene sequence similarities, respectively). The chemotaxonomic properties of strain F3212T were similar to those of members of the genus Marinospirillum. Q-8 was the sole respiratory ubiquinone and the genomic DNA G+C content was 53.3 mol%. The major fatty acids were C18 : 1 ω9c, C16 : 0 and C18 : 0. The polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentifed lipid and an unidentified aminophospholipid. The average nucleotide identity scores for strains M. celere DSM 18438T and M. minutulum DSM 6287T were 74.5 and 69.4 %, respectively. The DNA-DNA homologies with M. celere DSM 18438T and M. minutulum DSM 6287T were less than 20 %. It's concluded that strain F3212T represents a new species of the genus Marinospirillum, for which the name Marinospirillum perlucidum sp. nov. is proposed. The type strain is F3212T (=KCTC 52892T=MCCC 1H00198T).


Assuntos
Sedimentos Geológicos/microbiologia , Oceanospirillaceae/classificação , Filogenia , Tanques/microbiologia , Pepinos-do-Mar/microbiologia , Animais , Aquicultura , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Hibridização de Ácido Nucleico , Oceanospirillaceae/isolamento & purificação , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Água do Mar/microbiologia , Análise de Sequência de DNA , Ubiquinona/química
12.
Int J Syst Evol Microbiol ; 69(12): 3745-3750, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31433290

RESUMO

In the present paper, we describe a new species of the genus Flavobacterium, designated as strain 1E403T, which was isolated from a soil sample collected from the Arctic. Strain 1E403T was Gram-stain-negative, yellow-pigmented, rod-shaped, gliding and aerobic. Growth occurred at 4-37 °C (optimum, 28 °C), pH 6.0-9.0 (pH 7.0) and with 0-2 % (w/v) NaCl (0 %) on modified marine agar 2216. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that strain 1E403T was affiliated with the genus Flavobacterium and was more closely related to Flavobacterium subsaxonicum DSM 21790T (96.6 %) than to other species. In silico genomic comparisons, including average nucleotide identity and the digital DNA-DNA hybridization values, showed 73.9 % and 18.8 % identity to the closest relative Flavobacterium subsaxonicum DSM 21790T, respectively. The average amino acid identity value between strain 1E403T and Flavobacterium aquatile DSM 1132T was 63.8 %. The DNA G+C content of the strain was 36.8 %, while the sole respiratory quinone was menaquinone-6. Iso-C15 : 0 and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) were the major fatty acids (>10 %) of strain 1E403T. The polar lipid profile of strain 1E403T contained phosphatidylethanolamine, four unidentified aminolipids and two unidentified phospholipids. Based on the phenotypic characteristics, chemotaxonomic characteristics and phylogenetic inference, strain 1E403T represents a novel species of the genus Flavobacterium, and we propose the name Flavobacterium cerinum sp. nov. The type strain of Flavobacterium cerinum sp. nov. is 1E403T (=KCTC 62960T=MCCC 1H00356T).


Assuntos
Flavobacterium/classificação , Filogenia , Microbiologia do Solo , Tundra , Regiões Árticas , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Flavobacterium/isolamento & purificação , Hibridização de Ácido Nucleico , Fosfolipídeos/química , Pigmentação , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/química
13.
Int J Syst Evol Microbiol ; 69(11): 3437-3442, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31385781

RESUMO

A Gram-stain-negative, non-motile, aerobic, short rod-shaped bacterium, designated OD32T, was isolated from a soil sample taken from the rhizosphere of Silene acaulis collected from the Arctic tundra. The novel strain, OD32T, was able to grow at 15-37 °C (optimum, 33 °C), pH 6.5-9.5 (pH 7.5-8.0) and 0-0.5 % NaCl (0 %, w/v). A phylogenetic analysis based on 16S rRNA gene sequences revealed that strain OD32T was most closely related to the genus Deinococcus; it shared the highest sequence similarity (95.7 %) with Deinococcus grandis ATCC 43672T. Genomic DNA G+C content of the strain OD32T was 68.2 mol%. The predominant respiratory quinone was menaquinone 8 (MK-8). The major fatty acids were summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c), C15 : 1 ω6c, C17 : 1 ω6c, C17 : 0, C16 : 0 and iso-C17 : 0. Based on phylogenetic inference and genotypic characteristics, strain OD32T represents a novel species of the genus Deinococcus, for which the name Deinococcus arcticus sp. nov. is proposed. The type strain is OD32T (=KCTC 33972T=CICC 24671T).


Assuntos
Deinococcus/classificação , Filogenia , Rizosfera , Silene/microbiologia , Microbiologia do Solo , Tundra , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Deinococcus/isolamento & purificação , Ácidos Graxos/química , Genótipo , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Svalbard
14.
Int J Syst Evol Microbiol ; 69(10): 3056-3061, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31310196

RESUMO

A novel Gram-stain-negative, facultatively anaerobic, oxidase-negative, catalase-positive bacillus, designated WD2A32T, was isolated from a marine solar saltern in the coast of Weihai, Shandong Province, PR China. Strain WD2A32T grew optimally at 37-40 °C (range, 20-45 °C) and pH 7.0-7.5 (range, 6.5-8.0) and was tolerant to 6-8 % (w/v) NaCl (range, 2-12 %). The 16S rDNA of strain WD2A32T was most similar (93.5 %) to that of Rhodovibrio salinarumDSM 9154T (the type species of the genus Rhodovibrio), followed by Limimonas halophilaDSM 25584T (92.4 %; the type species of the genus Limimonas). The similarities to the type strains of the genera Pelagibiusand Limibacilluswere less than 91.0 %. The draft genome sequence of strain WD2A32T contained 72 contigs (>507 bp) of 4 237 996 bp with a DNA G+C content of 65.5 mol%. The major polar lipids of strain WD2A32T were phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and unidentified aminolipids; major cellular fatty acids were summed feature 8 (C18 : 1ω7c/C18 : 1ω6c) and C19 : 0 cyclo ω8c; the major respiratory quinone was Q-10. On the basis of the results from chemotaxonomic, physiological and biochemical analyses, strain WD2A32T represents a novel species of a novel genus in the family Rhodospirillaceae, for which the name Ferruginivarius sediminum gen. nov., sp. nov. has been proposed. The type strain is WD2A32T (=KCTC 52888T=MCCC 1H00201T).


Assuntos
Filogenia , Rhodospirillaceae/classificação , Salinidade , Microbiologia da Água , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Rhodospirillaceae/isolamento & purificação , Análise de Sequência de DNA , Ubiquinona/análogos & derivados , Ubiquinona/química
15.
J Agric Food Chem ; 67(32): 8875-8883, 2019 Aug 14.
Artigo em Inglês | MEDLINE | ID: mdl-31347830

RESUMO

Glucan synthase (GLS) gene is known to be involved in the fungal biosynthesis of cell wall, differentiation, and growth. In the present study, a glucan synthase gene (GFGLS) in the edible mushroom Grifola frondosa with a full sequence of 5927 bp encoding a total of 1781 amino acids was cloned and characterized for the first time. GFGLSp is a membrane protein containing two large transmembrane domains connected with a hydrophilic cytoplasmic domain. With a constructed dual promoter RNA silencing vector pAN7-gfgls-dual, a GFGLS-silencing transformant iGFGLS-3 had the lowest GFGLS transcriptional expression level (26.1%) with a shorter length and thinner appearance of the mycelia, as well as decreased mycelial biomass and exo-polysaccharide production of 5.02 and 0.38 g/L, respectively. Further analysis indicated that GFGLS silence influenced slightly the monosaccharide compositions and ratios of mycelial and exo-polysaccharide. These findings suggest that GFGLS could affect mycelial growth and polysaccharide production by downregulating the glucan synthesis.


Assuntos
Polissacarídeos Fúngicos/biossíntese , Proteínas Fúngicas/metabolismo , Glucosiltransferases/metabolismo , Grifola/enzimologia , Micélio/crescimento & desenvolvimento , Proteínas Fúngicas/genética , Glucosiltransferases/genética , Grifola/genética , Grifola/crescimento & desenvolvimento , Grifola/metabolismo , Micélio/enzimologia , Micélio/genética , Micélio/metabolismo , Interferência de RNA , RNA Interferente Pequeno/genética , RNA Interferente Pequeno/metabolismo
16.
Int J Syst Evol Microbiol ; 69(9): 2767-2774, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31237538

RESUMO

In this study, two bacterial strains designated B210T and SEH01T, isolated from coastal sediment sampled in Weihai, PR China, were characterized using a polyphasic approach. Strains were Gram-stain-negative, facultative anaerobic, rod-shaped and motile. According to the results of phylogenetic analyses based on their 16S rRNA genes, these two strains should be classified under the order Bradymonadales and they both show <90 % sequence similarities with Bradymonas sediminis FA350T. Moreover, strain B210T showed 98.6 % sequence similarity to strain SEH01T. Genomic characteristics including average nucleotide identity and in silico DNA-DNA hybridization values clearly separated strain B210T from strain SEH01T. The sole quinone of these two strains was menaquinone MK-7, and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified lipid. Iso-C15 : 0 was the major fatty acid in both strains B210T and SEH01T, and iso-C14 : 0 3-OH was also a major fatty acid for strain SEH01T. Based on the polyphasic analysis, these two strains represent two novel species of a new genus within the family Bradymonadaceae. Consequently, the novel genus Lujinxingia gen. nov. is proposed, containing two new species Lujinxingia litoralis gen. nov. sp. nov. and Lujinxingia sediminis sp. nov., with strain B210T (=KCTC 42951T=CGMCC 1.16770T) and strain SEH01T (=KCTC 42950T=DSM 101859T) as the type strains, respectively.


Assuntos
Deltaproteobacteria/classificação , Sedimentos Geológicos/microbiologia , Filogenia , Água do Mar/microbiologia , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Deltaproteobacteria/isolamento & purificação , Ácidos Graxos/química , Hibridização de Ácido Nucleico , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/química
17.
Int J Syst Evol Microbiol ; 69(8): 2388-2394, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31150324

RESUMO

A facultatively anaerobic, Gram-stain-negative, non-motile and straight to slightly curved long rod-shaped bacterial strain, designated XSD2T, was isolated from coastal seawater of Xiaoshi Island, PR China. The cells were catalase-positive, oxidase-negative and non-flagellated. Strain XSD2T was found to grow at 20-40 °C (optimum, 33 °C), at pH 6.0-8.5 (pH 7.0-7.5) and with 1-5 % (w/v) NaCl (3 %). Carotenoid pigments were produced. The major cellular fatty acids (>10.0 %) were iso-C15 : 0, iso-C16 : 0 3-OH and C17 : 1ω6c and the major polar lipids were phosphatidylethanolamine, one unidentified aminolipid and three unidentified polar lipids. The sole respiratory quinone was MK-7 and the genomic DNA G+C content was 44.1 mol%. The result of the 16S rRNA gene sequence analysis confirmed the affiliation of this organism to the order Marinilabiliales, family Prolixibacteraceae, with Mariniphaga sediminis SY21T as its closest relative with only 93.6 % sequence similarity. On the basis of physiological, biochemical and chemotaxonomic characteristics, we propose that strain XSD2T (=KCTC 62994T=MCCC 1H00347T) represents a novel species of a novel genus in the family Prolixibacteraceae, for which the name Maribellus luteus gen. nov., sp. nov. is proposed.


Assuntos
Bacteroidetes/classificação , Filogenia , Água do Mar/microbiologia , Técnicas de Tipagem Bacteriana , Bacteroidetes/isolamento & purificação , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Ilhas , Fosfatidiletanolaminas/química , Pigmentação , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/química
18.
Int J Syst Evol Microbiol ; 69(7): 1926-1933, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31157612

RESUMO

A novel bacterial strain, JDX94T, was isolated from tundra soil sampled north of the Yellow River station, Arctic. Cells were Gram-stain-negative, non-spore-forming, short rod-shaped and aerobic. The strain displayed growth at 4-37 °C with an optimum at 28 °C, with 0-1.0 % (w/v) NaCl (optimum, 0%) and at pH 6.0-9.0 (optimum, pH 7.0-7.5). Cells contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C17 : 0 3-OH as its major cellular fatty acids and menaquinone-7 as the only respiratory quinone. The polar lipid profile of strain JDX94T consisted of phosphatidylethanolamine, two unidentified aminolipids and four unknown polar lipids. The DNA G+C content was 37.5 mol%. On the basis 16S rRNA gene sequence comparison, strain JDX94T showed the highest sequence similarity (96.7 %) to Pedobacteragri JCM 15120T, followed by Pedobacteralluvionis DSM 19624T (96.3 %). Furthermore, the average nucleotide identity and digital DNA-DNA hybridization values between strain JDX94T and related species of the genus Pedobacter were 74.6-79.2 % and 18.9-24.5 %, respectively. Based on the presented results, we propose a novel species for which the name Pedobacterchinensis sp. nov. is suggested, with the type strain JDX94T (=MCCC 1H00335T= KCTC 62850T).


Assuntos
Celulose/metabolismo , Pedobacter/classificação , Filogenia , Microbiologia do Solo , Tundra , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Hibridização de Ácido Nucleico , Pedobacter/isolamento & purificação , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Svalbard , Vitamina K 2/análogos & derivados , Vitamina K 2/química
19.
Int J Syst Evol Microbiol ; 69(7): 2037-2042, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31066657

RESUMO

A novel blush-red-pigmented, Gram-stain-negative, gliding, aerobic and rod- or oval-shaped bacterium, designated strain 12N15T, was isolated from sediment sampled at a marine saltern located in Wendeng, China (36° 59' 56.49″ N, 122° 1' 38.84″ E). Growth was observed at 10-40 °C (optimum, 28 °C), in 1.0-12.0 % NaCl (2.0-5.0 %, w/v) and at pH 6.0-9.5 (pH 7.0). The respiratory quinones were determined to be Q-10 and major fatty acids were C18 : 1ω7c and C18 : 0. The polar lipids profile of strain 12N15T included phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, aminolipid, phosphatidylcholine, one lipid and three phospholipids. The genomic DNA G+C content was 69.6 mol%. Phylogenetic analyses based on the 16S rRNA gene showed that the strain 12N15T was affiliated within the genus Jannaschia, and was most closely related to Jannaschia seohaensis KCTC 22172T. The average amino acid identity and percentage of conserved protein values between strain 12N15T and the type strain of the type species, Jannaschia helgolandensis DSM 14858T, were 70.2 % and 64.1 %, respectively. The average nucleotide identity value between strain 12N15T and J.annaschia seohaensis KCTC 22172T was 81.9 %. The phenotypic, phylogenetic and genomic analyses supported the hypothesis that strain 12N15T represents a novel species of the genus Jannaschia, for which the name Jannaschia formosa sp. nov. is proposed. The type strain is 12N15T (=MCCC 1H00325T=KCTC 62582T).


Assuntos
Sedimentos Geológicos/microbiologia , Filogenia , Rhodobacteraceae/classificação , Salinidade , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Rhodobacteraceae/isolamento & purificação , Análise de Sequência de DNA
20.
Int J Syst Evol Microbiol ; 69(3): 701-707, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30694173

RESUMO

A Gram-stain negative, spherical, obligately aerobic bacterium, designated strain WN38T, was isolated from a marine solar saltern on the coast of Weihai, China. Optimal growth occurred at 33 °C, pH 7.0-7.5 and in the presence of 3-4 % (w/v) NaCl. The genome of strain WN38T was found to contain the genes necessary for arsenate reductase and related proteins, indicating that it may have potential in bioremediation of heavy metal polluted environments. Comparative 16S rRNA gene sequence analysis showed that strain WN38T represented a member of the genus Coraliomargarita, and was related most closely to Coraliomargarita akajimensis KCTC 12865T (95.7 %). Pairwise sequence similarities to all other type strains of species were below 90 %. Genome-based calculations (average nucleotide identity, genome-to-genome distance and DNA G+C percentage) and results of pairwise amino acid identity (AAI >60 %) and percentage of conserved proteins (POCP >50 %) also indicated clearly that strain WN38T represents a novel species within this genus. Different phenotypic analyses, such as the detection of a quinone system composed of the sole respiratory quinone was menaquinone-7 (MK-7) and a fatty acid profile with iso-C14 : 0, C18 : 0 and C18 : 1ω9c as major components, supported this finding at the same time as contributing to a comprehensive characterization of strain WN38T. On the basis of its phenotypic and genotypic properties, strain WN38T represents a novel species of the genus Coraliomargarita, for which the name Coraliomargaritasinensis sp. nov. is proposed. The type strain is WN38T (=KCTC 62602T=MCCC 1H00313T).


Assuntos
Filogenia , Águas Salinas , Verrucomicrobia/classificação , Microbiologia da Água , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Verrucomicrobia/isolamento & purificação , Vitamina K 2/análogos & derivados , Vitamina K 2/química
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