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1.
Front Psychol ; 11: 166, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32116960

RESUMO

During the recognition of faces, the incoming perceptual information is matched against mental representations of familiar faces stored in memory. Face space models describe an abstract concept of face representations and their mental organization, in which facial representations are located on various characteristic dimensions, depending on their specific facial characteristics. However, these models are defined just as incompletely as the general understanding of face recognition. We took two phenomena from face processing to better understand face recognition, and so the nature of face space: face adaptation and face priming. The face literature has mainly focused on face adaptation, largely neglecting face priming when trying to integrate outcomes regarding face recognition into the face space framework. Consequently, the present paper aims to review both phenomena and their contributions to face recognition, representation, and face space.

2.
J Biotechnol ; 186: 110-8, 2014 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-25014403

RESUMO

Fed-batch culture bioprocesses are currently used predominantly for the production of recombinant proteins, especially monoclonal antibodies. In these cultures, concentrated feeds are added during cultivation to prevent nutrient depletion, thus extending the cellular growth phase and increasing product concentrations. One limitation in these bioprocesses arises from the low solubility or stability of some compounds at high concentrations, in particular amino acids. This study describes the synthesis and evaluation of a phosphotyrosine disodium salt as a tyrosine source in fed-batch processes. This molecule is highly soluble in concentrated feeds at neutral pH. Mechanistic studies demonstrated that the molecule is cleaved in the cell culture supernatant after processing by released phosphatases, leading to phosphate and free L-tyrosine which can be taken up by the cells. No intact phosphotyrosine was detected intracellularly or incorporated into the sequence of the monoclonal antibody. The use of this new molecule allows the simplification of fed-batch processes in large scale manufacturing via the implementation of neutral pH, highly concentrated feeds.


Assuntos
Anticorpos Monoclonais/metabolismo , Reatores Biológicos , Meios de Cultura/química , Fosfotirosina/química , Proteínas Recombinantes/metabolismo , Sódio/química , Animais , Anticorpos Monoclonais/química , Células CHO , Cricetinae , Cricetulus , Meios de Cultura/metabolismo , Fosfotirosina/metabolismo , Proteínas Recombinantes/química , Sódio/metabolismo
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