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1.
Food Chem ; 304: 125403, 2020 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-31479993

RESUMO

Current information on the links between the chemistry and hedonic liking of edible mushrooms is scarce. In this study, 84 consumers evaluated the appearance, odor, taste, texture and overall liking of samples of Nordic edible wild mushroom species. Subsequently, multivariate models on the effects of non-volatile compounds, odor-contributing volatile compounds, sensory attributes and hedonic likings were created. The non-volatile compounds were measured with quantitative NMR. The five studied mushroom species were different in their sugar and acid contents. Three consumer clusters were found with species*cluster interactions. Correlations with sensory attributes and chemical components were found, and the multivariate models indicated predictor attributes for each consumer cluster. The results indicate that the sensory properties could be correlated to both volatile and non-volatile compounds, there are consumer clusters with differing likings as regards mushrooms, and these clusters are heterogenic groups with no simple factors such as age explaining their liking scores.

2.
Food Res Int ; 125: 108551, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31554128

RESUMO

The camu-camu seeds, which comprehend about 20% of the fruit weight, is discarded without taking benefit of their chemical components and potential application by the industry. In the current study, we characterized the phenolic composition, the in vitro chemical antioxidant effects, cytotoxic activity, and the inhibition of induced-cisplatin chromosomal aberrations of five camu-camu seed extracts obtained with different proportions of water (H2O) and ethyl alcohol (EtOH). The 50% H2O + 50% EtOH was the most promising extract because it presented higher total phenolic content (4802 mg GAE/100 g), antioxidant capacity (DPPH = 3694 mg AAE/100 g; FRAP = 6604 mg AAE/100 g; FCRC = 4918 mg GAE/100 g) and inhibited the cell growth of four cancer cell lines (GI50 = 7.49 µg GAE/mL A549; 13.3 µg GAE/mL Caco-2; 15.57 µg GAE/mL HepG2 and 14.89 µg GAE/mL HCT8) without cytotoxic effects against normal cells (GI50 IMR90 > 43.2 µg GAE/mL). The cytotoxic effects presented high correlation with the (-)-epicatechin and methylvescalagin contents, while gallic and 2,5-dihydroxybenzoic acids were associated with cytoprotective effects of HCT8 cancer cell line. The 50% H2O + 50% EtOH extract also presented protective effect by decreasing 37% of the induced-cisplatin chromosomal breaks index, suggesting its antimutagenic potential, which may be associated to its antioxidant and cytotoxic activities.

3.
J Oleo Sci ; 68(7): 671-677, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31178467

RESUMO

Conifer resins are used as chemical raw materials for daily necessities. There have been many reports on the aroma components of turpentine oil from rosin, but there has been no reports on fluctuations in the aroma components through spring to late autumn. We speculated that the aroma components in the essential oils of deciduous coniferous larches might fluctuate during maturation of the foliage. In this study, we focused on the aroma components of larch essential oils and we clarified by multivariate analysis how the aroma components fluctuate during leaf maturation. The results of analysis showed that there was a drastic seasonal fluctuation of the chemical components in larch essential oils, especially in senescent leaves in late autumn. Cryptone and linalool were identified as the characteristic aroma components in essential oil from senescent larch leaves.


Assuntos
Larix/química , Odorantes/análise , Óleos Voláteis/química , Folhas de Planta/química , Estações do Ano , Terebintina/química , Monoterpenos/isolamento & purificação , Óleos Voláteis/isolamento & purificação , Folhas de Planta/crescimento & desenvolvimento , Resinas Vegetais
4.
Data Brief ; 20: 573-576, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30191170

RESUMO

This article describes the inhibitory effects of extracts from 25 plants harvested in Sri Lanka against tyrosinase and collagenase. Inhibitors of these enzymes are common ingredients in cosmetics and medications, which help protect the skin against hyperpigmentation and premature aging. The article also discusses the polyphenol content of the extracts, which is well known to possess antioxidant properties. The extract data from the following plants, which have a long history in Sri Lankan traditional medicine, such as Ayurveda, have been provided: English name, "local name in Sri Lanka," (scientific name). Indian copperleaf plant, "kuppameniya," (Acalypha indica); red sandalwood, "madatiya", (Adenanthera pavonina); balipoovu plant, "polpala," (Aerva lanata); snap ginger, "heen araththa," (Alpinia calcarata); bael fruit, "beli," (Aegle marmelos); coastal waterhyssop, "lunuwila," (Bacopa monnieri); porcupine flower, "katu karandu," (Barleria prionitis); balloon-vine plant, "wel penera," (Cardiospermum halicacabum); water caltrop, "Katupila," (Flueggea leucopyrus); Indian sarsparilla, "iramusu," (Hemidesmus indicus); malabar nut plant, "adhatoda," (Justicia adhatoda); wood apple, "divul," (Limonia acidissima); holy basil plant, "maduruthala," (Ocimum tenuiflorum); emblic myrobalan plant, "nelli," (Phyllanthus emblica); long pepper plant,"thippili," (Piper longum); country borage plant, "kapparawalliya," (Plectranthus amboinicus); common sesban, "wel murunga," (Sesbania sesban); turkey berry, "gona batu," (Solanum rudepannum Dunal); purple fruited pea eggplant,"welthibbatu," (Solanum trilobatum); black plum, "madan," (Syzygium cumini); crape jasmine, "wathusudda," (Tabernaemontana divaricate); purple tephrosia, "pila," (Tephrosia purpurea); Chinese chaste tree, "nika," (Vitex negundo); and arctic snow, "suduidda," (Wrightia antidysenterica). The inhibitory effects of these plant extracts on tyrosinase and collagenase, as well as polyphenol contents in the extracts, are detailed in Table 1.

5.
Data Brief ; 19: 393-397, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-29900335

RESUMO

We analyzed the volatile compounds produced by serotype D Clostridium botulinum (D-CB16) in trypticase peptone/yeast extract/glucose (TYG) medium using gas chromatography/mass spectrometry (GC/MS). The volatile compounds were captured by solid-phase microextraction and applied to GC/MS for separation and identification of the compounds in TYG medium with or without the cultivation of C. botulinum D-CB16. Thirty-five and 34 volatile compounds were identified in media without and with D-CB16 cultivation, respectively. Of the compounds identified in the medium with the strain, twenty-one were not detected in the original medium, indicating that these were produced by C. botulinum D-CB16.

6.
Data Brief ; 16: 993-999, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29322080

RESUMO

This data article describes the flow-mediated vasodilation (FMD) responses, represented by changes in arterial diameter, and blood pressure changes in young adults after a single oral dose of camu camu (Myrciaria dubia) pericarp extract or placebo (cross-over design). Ten healthy men and 10 healthy women participated in this study. Ultrasonic diagnostic equipment was used to monitor arterial diameter changes, indicative of FMD, for 110 s after the administration of the camu camu extract or placebo. In addition, the systolic and diastolic blood pressure values were recorded.

7.
J Nat Med ; 72(1): 375-380, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29188415

RESUMO

The petals or buds of Rosa species have been used as an important Chinese crude drug called "Mei-gui Hua" and also an ingredient for herbal tea in China. The 15 flavonol glycosides in 34 commercially available "Mei-gui Hua" were quantitatively determined by UPLC, and the data were compared with those of known Rosa sp. belong to Cinnamomeae, Gallicanae, Caninae and Synstylae by principal component analysis for the estimation of original plants of these "Mei-gui Hua". Seven samples were classified into two groups (Types A and B) composed of species in Gallicae and Synstilae and 11 samples into a group (Type D) composed of species in Cinnamomeae. Six samples were plotted among Types B, C and D. However, nine samples were shown to form a new group (Type F), and the original plants of these samples were assumed to be more complex hybrids of Rosa species including other sections than Cinnamomeae, Gallicanae, Caninae and Synstylae. This method must be useful and convenient to estimate the origin of crude drug "Mei-gui Hua" commercially available in markets. The results of this study also demonstrated that "Mei-gui Hua" commercially available in Chinese market must be prepared from hybrids of various species of Rosa, and it is not reasonable to assign only R. rugosa as scientific name of the origin plants.


Assuntos
Medicamentos de Ervas Chinesas/isolamento & purificação , Glicosídeos/isolamento & purificação , Quercetina/análogos & derivados , Quercetina/isolamento & purificação , Rosa/química , China , Flores/química
8.
Biosci Biotechnol Biochem ; 81(8): 1461-1465, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28475419

RESUMO

Various antimicrobial constituents of camu-camu fruit were isolated. Acylphloroglucinol (compound 1) and rhodomyrtone (compound 2) were isolated from the peel of camu-camu (Myrciaria dubia) fruit, while two other acylphloroglucinols (compounds 3 and 4) were obtained from camu-camu seeds. The structures of the isolated compounds were characterized by spectrophotometric methods. Compounds 1 and 4 were confirmed to be new acylphloroglucinols with different substituents at the C7 or C9 position of 2, and were named myrciarone A and B, respectively. Compound 3 was determined to be isomyrtucommulone B. This is the first report of the isolation of 3 from a natural resource. The antimicrobial activities of compounds 1, 3, and 4 were similar to those of 2, and the minimum inhibitory concentrations were either similar to or lower than that of kanamycin. These results suggest that the peel and seeds of camu-camu fruit could be utilized for therapeutic applications.


Assuntos
Anti-Infecciosos/química , Frutas/química , Myrtaceae/química , Floroglucinol/isolamento & purificação , Sementes/química , Xantonas/química , Anti-Infecciosos/isolamento & purificação , Anti-Infecciosos/farmacologia , Candida albicans/efeitos dos fármacos , Candida albicans/crescimento & desenvolvimento , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Negativas/crescimento & desenvolvimento , Bactérias Gram-Positivas/efeitos dos fármacos , Bactérias Gram-Positivas/crescimento & desenvolvimento , Canamicina/farmacologia , Testes de Sensibilidade Microbiana , Estrutura Molecular , Floroglucinol/análogos & derivados , Floroglucinol/farmacologia , Extratos Vegetais/química , Saccharomyces cerevisiae/efeitos dos fármacos , Saccharomyces cerevisiae/crescimento & desenvolvimento , Xantonas/isolamento & purificação , Xantonas/farmacologia
9.
J Oleo Sci ; 66(8): 843-849, 2017 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-28381767

RESUMO

The sedative effects of volatile components in the essential oil of Artemisia montana ("Yomogi") were investigated and measured using gas chromatography-mass spectrometry (GC-MS). Major components identified included 1,8-cineol, camphor, borneol, α-piperitone, and caryophyllene oxide. Among them, 1,8-cineol exhibited the highest flavor dilution (FD) value in an aroma extract dilution analysis (AEDA), followed by borneol, o-cymene, ß-thujone, and bornyl acetate. The sedative effects of yomogi oil aroma were evaluated by sensory testing, analysis of salivary α-amylase activity, and measurement of relative fluctuation of oxygenated hemoglobin concentration in the brain using near-infrared spectroscopy (NIRS). All results indicated the stress-reducing effects of the essential oil following nasal exposure, and according to the NIRS analysis, 1,8-cineol is likely responsible for the sedative effects of yomogi oil.


Assuntos
Aromaterapia , Artemia/química , Cicloexanóis/farmacologia , Hipnóticos e Sedativos/farmacologia , Monoterpenos/farmacologia , Óleos Voláteis/química , Fitoterapia , Óleos Vegetais/química , Estresse Psicológico/prevenção & controle , Administração Intranasal , Adulto , Animais , Encéfalo/metabolismo , Cicloexanóis/administração & dosagem , Cicloexanóis/isolamento & purificação , Eucaliptol , Feminino , Cromatografia Gasosa-Espectrometria de Massas , Hemoglobinas/metabolismo , Humanos , Hipnóticos e Sedativos/isolamento & purificação , Masculino , Monoterpenos/administração & dosagem , Monoterpenos/isolamento & purificação , Saliva/enzimologia , Espectroscopia de Luz Próxima ao Infravermelho , Volatilização , Adulto Jovem , alfa-Amilases/metabolismo
10.
Phytochemistry ; 107: 61-8, 2014 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-25220498

RESUMO

Thirteen flavonol glycosides were isolated from the petals of Rosa species belonging to the section Gallicanae, and their structures were identified from their spectroscopic data. These flavonol glycosides, along with two flavonol glycosides isolated from Rosa rugosa, in the petals of 31 Rosa species belonging to sections Gallicanae, Cinnamomeae, Caninae, and Synstylae were quantitatively analyzed by UPLC. The results indicated that the species belonging to these sections could be classified into four types (Type A, B, C and D) based on the pattern of flavonol glycoside contents, whereas the R. rugosa flavonol glycosides were detected only in section Cinnamomeae. A principal components analysis (PCA) calculated from the 15 flavonol glycosides contained in these samples supported the presence of four types. The distribution of the species in Type D (a group of Cinnamomeae) was shown to reflect close interrelationships, but species in Type B (one group of Gallicanae) could be subdivided into two groups, one of which contained species in section Synstylae. Moreover, the flavonol glycosides were grouped by sugar moieties: a disaccharide composed of two hexoses (S1), a hexose (S2), including a hexose with galloyl group, a pentose (S3), and a disaccharide composed of a hexose and a pentose (S4). The ratios of the amounts of S1-S4 to total flavonol glycoside content indicated that differences among the four sections were more distinctive than the amounts of the 15 flavonol glycosides. The 31 samples were divided into Type B, composed of one type of Gallicanae and Synstylae, Type A+C, composed of another type of Gallicanae and Caninae, and Type D, composed of Cinnamomeae. The R. rugosa flavonol glycosides were shown to be important chemotaxonomic markers for the classification of species in Cinnamomeae, and this method of using flavonol glycosides as chemotaxonomic markers could be useful for the identification of Rosa species belonging to sections Gallicanae, Cinnamomeae, Caninae, and Synstylae.


Assuntos
Flavonóis/isolamento & purificação , Glicosídeos/classificação , Glicosídeos/isolamento & purificação , Rosa/química , Flavonóis/análise , Flavonóis/química , Flavonóis/classificação , Flores/química , Glicosídeos/análise , Glicosídeos/química , Quempferóis/química , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Quercetina/química
11.
J Oleo Sci ; 62(9): 755-62, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24005020

RESUMO

Aromatic components in the essential oil prepared from the leaves of cultivated Myrica gale var. tomentosa were compared with those from oil derived wild plants by using gas chromatography-mass spectroscopy (GC/MS). We found that essential oils from both the wild and cultivated plants contained similar aromatic components such as ß-elemenone, selina 3,7(11)-diene, myrcene, limonene, cymene, 1,8-cineole, and ß-pinene, but the content ratio of the oil was significantly different, which might yield differences in the aromatic properties. The aroma impact components of the essential oils were also determined using GC/MS-Olfactometry (GC/MS-O) and aroma extract dilution analysis. Eight aromatic compounds, including linalool, limonene, and 1,8-cineole, were shown to contribute to the aromatic properties of cultivated M. gale var. tomentosa. The strongest aromatic note was defined as linalool, followed by limonene, 1,8-cineole, and ß-elemenone. The essential oil, ethanol (EtOH), 1,3-butylene glycol (BG), and 1,3-propanediol (PD) extracts prepared from the leaves of cultivated M. gale var. tomentosa also showed antioxidant and antimicrobial activities, that is, they demonstrated scavenger activity against hydroxyl and superoxide radicals in the aqueous phase, and showed inhibitory effects on lipid peroxidation. The essential oil extracts also exhibited antimicrobial activity against gram-positive bacteria, with the lowest minimum inhibitory concentration value against Bacillus subtilis. In conclusion, the essential oil and solvent extracts from cultivated M. gala var. tomentosa have a potential for utilization as food and cosmetic ingredients.


Assuntos
Anti-Infecciosos/farmacologia , Antioxidantes/farmacologia , Bactérias Gram-Positivas/efeitos dos fármacos , Myrica/química , Óleos Voláteis/isolamento & purificação , Óleos Voláteis/farmacologia , Bacillus subtilis/efeitos dos fármacos , Farmacorresistência Bacteriana , Depuradores de Radicais Livres , Cromatografia Gasosa-Espectrometria de Massas , Peroxidação de Lipídeos/efeitos dos fármacos , Olfatometria , Folhas de Planta/química
12.
Protein Expr Purif ; 54(2): 295-9, 2007 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-17459724

RESUMO

Cold-active beta-galactosidase from Arthrobacter psychrolactophilus strain F2 was overexpressed in Escherichia coli using the Cold expression system and the recombinant enzyme, rBglAp, was characterized. The purified rBglAp exhibited similar enzymatic properties to the native enzyme, e.g., (i) it had high activity at 0 degrees C, (ii) its optimum temperature and pH were 10 degrees C and 8.0, respectively, and (iii) it was possible to rapidly inactivate the rBglAp at 50 degrees C in 5 min. Moreover, rBglAp was able to hydrolyze both ONPG and lactose with K(m) values of 2.7 and 42.1mM, respectively, at 10 degrees C. One U of rBglAp could hydrolyze about 70% of the lactose in 1 ml of milk in 24h, and the enzyme produced trisaccharide from lactose. We conclude that rBglAp is a cold-active enzyme that is extremely heat labile and has significant potential application to the food industry.


Assuntos
Arthrobacter/enzimologia , beta-Galactosidase/biossíntese , beta-Galactosidase/fisiologia , Temperatura Baixa , Estabilidade Enzimática , Escherichia coli/metabolismo , Concentração de Íons de Hidrogênio , Lactose/metabolismo
13.
Int J Mol Med ; 15(2): 259-63, 2005 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-15647841

RESUMO

The enzymatic hydrolysates from pollen Cistus ladaniferus were digested and prepared using three kinds of enzymes (pepsin, trypsin, and papain) and the antioxidative properties were investigated. The yields, total phenolic contents, and protein contents of these hydrolysates were as follows: yields (about 21-45%), total phenolics (10.39-14.33 microg/mg sample powder), and proteins (129.62-137.35 microg/mg sample powder), respectively. The hydrolysates possessed strongly antioxidative and scavenging abilities against reactive oxygen species. The present studies revealed that hydrolysates from honeybee-collected pollen are of benefit not only to the materials of health food diets, but also to patients with various diseases such as cancer, cardiovascular diseases, and diabetes.


Assuntos
Antioxidantes/farmacologia , Cistus/metabolismo , Ácido Linoleico/metabolismo , Oxigênio/metabolismo , Amidinas/farmacologia , Antioxidantes/química , Compostos de Bifenilo , Eletroforese em Gel de Poliacrilamida , Concentração de Íons de Hidrogênio , Radical Hidroxila/química , Indicadores e Reagentes/farmacologia , Oxidantes/química , Papaína/química , Pepsina A/química , Fenol/química , Picratos/farmacologia , Pólen/química , Pólen/metabolismo , Hidrolisados de Proteína/química , Espécies Reativas de Oxigênio , Superóxidos/química , Fatores de Tempo , Tripsina/química
14.
Nahrung ; 48(3): 226-9, 2004 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-15285117

RESUMO

Three extracts, namely hot-water fraction (HWF), water-soluble fraction (WSF), and ethanol-soluble fraction (ESF), were prepared from fresh bee bread imported from Lithuania. The protein and total phenolic contents of these samples were very high. Among them, WSF at 100% concentration showed the highest antioxidative ability and scavenging ability. On the other hand, ESF at 10% concentration possessed the highest ability against 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radicals. Bee bread will apply more and more as health food and medicine due to its functional properties such as antioxidative ability and scavenging activities of reactive oxygen species.


Assuntos
Anti-Infecciosos/química , Antioxidantes/análise , Manipulação de Alimentos/métodos , Alimentos Orgânicos , Própole/química , Anti-Infecciosos/análise , Compostos de Bifenilo , Hidrazinas , Radical Hidroxila/metabolismo , Oxirredução , Fenóis/análise , Picratos , Própole/análise , Espécies Reativas de Oxigênio/metabolismo
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