Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 13 de 13
Filtrar
Mais filtros










Intervalo de ano de publicação
1.
J Appl Oral Sci ; 29: e20201074, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34586189

RESUMO

Hyperglycemia, a major characteristic of diabetes, is considered to play a vital role in diabetic complications. High glucose levels have been found to inhibit the mineralization of dental pulp cells. However, gene expression associated with this phenomenon has not yet been reported. This is important for future dental therapeutic application. OBJECTIVE: Our study aimed to investigate the effect of high glucose levels on mineralization of human dental pulp-derived cells (hDPCs) and identify the genes involved. METHODOLOGY: hDPCs were cultured in mineralizing medium containing 25 or 5.5 mM D-glucose. On days 1 and 14, RNA was extracted and expression microarray performed. Then, differentially expressed genes (DEGs) were selected for further validation using the reverse transcription quantitative polymerase chain reaction (RT-qPCR) method. Cells were fixed and stained with alizarin red on day 21 to detect the formation of mineralized nodules, which was further quantified by acetic acid extraction. RESULTS: Comparisons between high-glucose and low-glucose conditions showed that on day 1, there were 72 significantly up-regulated and 75 down-regulated genes in the high-glucose condition. Moreover, 115 significantly up- and 292 down-regulated genes were identified in the high-glucose condition on day 14. DEGs were enriched in different GO terms and pathways, such as biological and cellular processes, metabolic pathways, cytokine-cytokine receptor interaction and AGE-RAGE signaling pathways. RT-qPCR results confirmed the significant expression of pyruvate dehydrogenase kinase 3 (PDK3), cyclin-dependent kinase 8 (CDK8), activating transcription factor 3 (ATF3), fibulin-7 (Fbln-7), hyaluronan synthase 1 (HAS1), interleukin 4 receptor (IL-4R) and apolipoprotein C1 (ApoC1). CONCLUSIONS: The high-glucose condition significantly inhibited the mineralization of hDPCs. DEGs were identified, and interestingly, HAS1 and Fbln-7 genes may be involved in the glucose inhibitory effect on hDPC mineralization.


Assuntos
Polpa Dentária , Transcriptoma , Diferenciação Celular , Proliferação de Células , Células Cultivadas , Glucose , Humanos , Análise em Microsséries
2.
J. appl. oral sci ; 29: e20201074, 2021. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1340110

RESUMO

Abstract Hyperglycemia, a major characteristic of diabetes, is considered to play a vital role in diabetic complications. High glucose levels have been found to inhibit the mineralization of dental pulp cells. However, gene expression associated with this phenomenon has not yet been reported. This is important for future dental therapeutic application. Objective Our study aimed to investigate the effect of high glucose levels on mineralization of human dental pulp-derived cells (hDPCs) and identify the genes involved. Methodology hDPCs were cultured in mineralizing medium containing 25 or 5.5 mM D-glucose. On days 1 and 14, RNA was extracted and expression microarray performed. Then, differentially expressed genes (DEGs) were selected for further validation using the reverse transcription quantitative polymerase chain reaction (RT-qPCR) method. Cells were fixed and stained with alizarin red on day 21 to detect the formation of mineralized nodules, which was further quantified by acetic acid extraction. Results Comparisons between high-glucose and low-glucose conditions showed that on day 1, there were 72 significantly up-regulated and 75 down-regulated genes in the high-glucose condition. Moreover, 115 significantly up- and 292 down-regulated genes were identified in the high-glucose condition on day 14. DEGs were enriched in different GO terms and pathways, such as biological and cellular processes, metabolic pathways, cytokine-cytokine receptor interaction and AGE-RAGE signaling pathways. RT-qPCR results confirmed the significant expression of pyruvate dehydrogenase kinase 3 (PDK3), cyclin-dependent kinase 8 (CDK8), activating transcription factor 3 (ATF3), fibulin-7 (Fbln-7), hyaluronan synthase 1 (HAS1), interleukin 4 receptor (IL-4R) and apolipoprotein C1 (ApoC1). Conclusions The high-glucose condition significantly inhibited the mineralization of hDPCs. DEGs were identified, and interestingly, HAS1 and Fbln-7 genes may be involved in the glucose inhibitory effect on hDPC mineralization.


Assuntos
Humanos , Polpa Dentária , Transcriptoma , Diferenciação Celular , Células Cultivadas , Análise em Microsséries , Proliferação de Células , Glucose
3.
Int J Paediatr Dent ; 2020 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-33220141

RESUMO

BACKGROUND: Bonding before sealant application enhances retention on saliva-contaminated sound teeth; however, there are few studies of bonding's efficacy on sealant retention on occlusal caries. AIM: To evaluate a bonding agent's efficacy on sealant retention on occlusal caries and caries transition. DESIGN: One hundred twenty pairs of first permanent molars with occlusal caries (ICDAS 2-4) from 98 children aged 7.3-9.9 years were included. One molar was randomly selected to use bonding (Adper™ Single Bond 2, 3M ESPE) applied before sealant application (Helioseal® clear, Ivoclar Vivadent). Bonding was not used on the contralateral tooth. Retention was determined using Simonsen's criteria; caries transition was evaluated using ICDAS scores, DIAGNOdent values, and bitewings at baseline, 6, 12, and 24 months. Data were analyzed with McNemar's and Fisher's exact tests. RESULTS: Bonding agent use significantly increased sealant retention rates (P < .001). Bonded (B) sealants had a higher retention rate (83.3%) than non-bonded (NB) sealants (53.7%). The difference in the caries-transition rate was not significant between the groups (P > .05). Three teeth (1.4%; NB:B = 2:1) were restored due to radiographic dentin-caries progression. CONCLUSIONS: Using a bonding agent before applying sealant on permanent molar occlusal caries (ICDAS 2-4) significantly enhanced sealant retention. Caries progression was rarely detected at the 24-month follow-up.

4.
Braz Dent J ; 31(3): 298-303, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32667524

RESUMO

Diabetes is a group of metabolic disorders that can lead to damage and dysfunction of many organs including the dental pulp. Increased inflammatory response, reduction of dentin formation and impaired healing were reported in diabetic dental pulp. Hyperglycemia, which is a main characteristic of diabetes, was suggested to play a role in many diabetic complications. Therefore our aim was to investigate the effects of high glucose levels on proliferation, reactive oxygen species (ROS) production and odontogenic differentiation of human dental pulp cells (HDPCs). HDPCs were cultured under low glucose (5.5mM Glucose), high glucose (25 mM Glucose) and mannitol (iso-osmolar control) conditions. Cell proliferation was analyzed by MTT assay for 11 days. Glutathione and DCFH-DA assay were used to assess ROS and antioxidant levels after 24 h of glucose exposure. Odontogenic differentiation was evaluated and quantified by alizarin red staining on day 21. Expression of mineralization-associated genes, which were alkaline phosphatase, dentin sialophosphoprotein and osteonectin, was determined by RT-qPCR on day 14. The results showed that high glucose concentration decreased proliferation of HDPCs. Odontogenic differentiation, both by gene expression and mineral matrix deposit, was inhibited by high glucose condition. In addition, high DCF levels and low reduced glutathione levels were observed in high glucose condition. However, no differences were observed between mannitol and low glucose conditions. In conclusion, the results clearly showed the negative effect of high glucose condition on HDPCs proliferation and differentiation. Moreover, it also induced ROS production of HDPCs.


Assuntos
Fosfatase Alcalina , Polpa Dentária , Diferenciação Celular , Proliferação de Células , Células Cultivadas , Proteínas da Matriz Extracelular , Glucose , Humanos , Odontoblastos , Fosfoproteínas , Espécies Reativas de Oxigênio
5.
Braz. dent. j ; 31(3): 298-303, May-June 2020. tab, graf
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-1132307

RESUMO

Abstract Diabetes is a group of metabolic disorders that can lead to damage and dysfunction of many organs including the dental pulp. Increased inflammatory response, reduction of dentin formation and impaired healing were reported in diabetic dental pulp. Hyperglycemia, which is a main characteristic of diabetes, was suggested to play a role in many diabetic complications. Therefore our aim was to investigate the effects of high glucose levels on proliferation, reactive oxygen species (ROS) production and odontogenic differentiation of human dental pulp cells (HDPCs). HDPCs were cultured under low glucose (5.5mM Glucose), high glucose (25 mM Glucose) and mannitol (iso-osmolar control) conditions. Cell proliferation was analyzed by MTT assay for 11 days. Glutathione and DCFH-DA assay were used to assess ROS and antioxidant levels after 24 h of glucose exposure. Odontogenic differentiation was evaluated and quantified by alizarin red staining on day 21. Expression of mineralization-associated genes, which were alkaline phosphatase, dentin sialophosphoprotein and osteonectin, was determined by RT-qPCR on day 14. The results showed that high glucose concentration decreased proliferation of HDPCs. Odontogenic differentiation, both by gene expression and mineral matrix deposit, was inhibited by high glucose condition. In addition, high DCF levels and low reduced glutathione levels were observed in high glucose condition. However, no differences were observed between mannitol and low glucose conditions. In conclusion, the results clearly showed the negative effect of high glucose condition on HDPCs proliferation and differentiation. Moreover, it also induced ROS production of HDPCs.


Resumo O diabetes abrange um grupo de distúrbios metabólicos que podem levar a danos e disfunções de muitos órgãos, incluindo a polpa dentária. Aumento da resposta inflamatória, redução da formação de dentina e comprometimento da cicatrização foram relatados na polpa dentária diabética. A hiperglicemia, que é uma característica determinante do diabetes, desempenha um papel importante em muitas complicações diabéticas. Portanto, nosso objetivo foi investigar os efeitos dos altos níveis de glicose na proliferação, produção de espécies reativas de oxigênio (ROS, em inglês) e diferenciação odontogênica das células da polpa dental humana (HDPCs, em inglês). As HDPCs foram cultivadas em condições de baixa glicose (glicose 5,5 mM), alta glicose (glicose 25 mM) e manitol (controle iso-osmolar). A proliferação celular foi analisada pelo ensaio MTT por 11 dias. Glutationa e DCFH-DA foram utilizados para avaliar os níveis de ROS e antioxidantes após 24 h de exposição à glicose. A diferenciação odontogênica foi avaliada e quantificada pela coloração com vermelho de alizarina no dia 21. A expressão de genes associados à mineralização, que eram fosfatase alcalina, sialofosfoproteína de dentina e osteonectina, foi determinada por RT-qPCR no dia 14. Os resultados mostraram que a alta concentração de glicose diminuiu a proliferação de HDPCs. A diferenciação odontogênica, tanto pela expressão gênica quanto pelo depósito da matriz mineral, foi inibida pela condição de alta glicose. Além disso, altos níveis de DCF e níveis reduzidos de glutationa foram observados na condição de alta glicose. No entanto, não foram observadas diferenças entre o manitol e as condições de baixa glicose. Em conclusão, os resultados mostraram claramente o efeito negativo da condição de alta glicose na proliferação e diferenciação de HDPCs. Além disso, essa condição também induziu a produção de ROS em HDPCs.


Assuntos
Humanos , Polpa Dentária , Fosfatase Alcalina , Fosfoproteínas , Diferenciação Celular , Células Cultivadas , Proteínas da Matriz Extracelular , Espécies Reativas de Oxigênio , Proliferação de Células , Glucose , Odontoblastos
6.
Community Dent Oral Epidemiol ; 46(3): 280-287, 2018 06.
Artigo em Inglês | MEDLINE | ID: mdl-29380407

RESUMO

Early Childhood Caries (ECC) is prevalent around the world, but in particular the disease is growing rapidly in low- and middle-income countries in parallel with changing diet and lifestyles. In many countries, ECC is often left untreated, a condition which leads to pain and adversely affects general health, growth and development, and quality of life of children, their families and their communities. Importantly, ECC is also a global public health burden, medically, socially and economically. In many countries, a substantial number of children require general anaesthesia for the treatment of caries in their primary teeth (usually extractions), and this has considerable cost and social implications. A WHO Global Consultation with oral health experts on "Public Health Intervention against Early Childhood Caries" was held on 26-28 January 2016 in Bangkok (Thailand) to identify public health solutions and to highlight their applicability to low- and middle-income countries. After a 3-day consultation, participants agreed on specific recommendations for further action. National health authorities should develop strategies and implement interventions aimed at preventing and controlling ECC. These should align with existing international initiatives such as the Sixtieth World Health Assembly Resolution WHA 60.17 Oral health: action plan for promotion and integrated disease prevention, WHO Guideline on Sugars and WHO breastfeeding recommendation. ECC prevention and control interventions should be integrated into existing primary healthcare systems. WHO public health principles must be considered when tackling the effect of social determinants in ECC. Initiatives aimed at modifying behaviour should focus on families and communities. The involvement of communities in health promotion, and population-directed and individual fluoride administration for the prevention and control of ECC is essential. Surveillance and research, including cost-effectiveness studies, should be conducted to evaluate interventions aimed at preventing ECC in different population groups.


Assuntos
Cárie Dentária/prevenção & controle , Odontologia em Saúde Pública , Organização Mundial da Saúde , Pré-Escolar , Congressos como Assunto , Cárie Dentária/epidemiologia , Humanos , Prevalência
7.
Southeast Asian J Trop Med Public Health ; 47(5): 1105-11, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29620824

RESUMO

This study aimed to evaluate the prevalence and factors associated with dental fluorosis in permanent incisors among children aged 8-10 years in Bangkok, Thailand. We studied 707 children selected by stratified randomization and examined each of them to determine their Dean's fluorosis index. Parents of the studied children were asked to fill out a questionnaire. The study was conducted from July to October 2014. Chi-square test and multiple logistic regression analysis were used to study the association between various factors and fluorosis among study subjects. Dental fluorosis was found in 18.4% of subjects. Sixteen point one percent and 2.3% of 707 subjects had very mild fluorosis and mild fluorosis, respectively. Factors significantly associated with dental fluorosis were family income (aOR=1.77; 95% CI: 1.10-2.86, p=0.02) and history of taking fluoride supplements (aOR=1.66; 95% CI: 1.07-2.61, p=0.03). This study indicated that the prevalence of fluorosis had increased in Bangkok, but the majority was very mild level.


Assuntos
Fluoretos/efeitos adversos , Fluorose Dentária/epidemiologia , Fluorose Dentária/etiologia , Criança , Estudos Transversais , Água Potável/química , Feminino , Fluoretos/química , Humanos , Masculino , Prevalência , Fatores de Risco , Tailândia/epidemiologia
8.
Artigo em Inglês | MEDLINE | ID: mdl-20578562

RESUMO

The objectives of this study were to determine the prevalence of early childhood caries and the oral health status in children aged 36-47 months in Vientiane, the capital city of Lao PDR. This study also aimed to assess the feeding practices, snack consumption, oral hygiene practices, and dental visits of children at this age. A total of 400 children were selected for oral examination and their caretakers were interviewed. The results showed the prevalence of dental caries was 82% with mean decayed, missing and filled teeth (dmft) +/- SD 5.5 +/- 4.3 teeth. No missing teeth were found (mt = 0), and the mean filled teeth (ft) was only 0.02. The teeth most affected by dental caries were the upper right and left central incisors, followed by the upper right and left lateral incisors, lower molars, upper molars, upper canines, lower canines and lower central incisors. The least affected were the lower right and left lateral incisors. Factors that affected dmft were tooth brushing frequency, brushing with parental assistance or supervision, time brushing started, feeding pattern, and the type of milk, candy and sweet beverages consumption. It can be concluded that children in the studied area had a high prevalence of caries and a high level of severity. Thus, oral health care programs should be promoted in Vientiane.


Assuntos
Cárie Dentária/epidemiologia , Pré-Escolar , Assistência Odontológica para Crianças/estatística & dados numéricos , Cárie Dentária/prevenção & controle , Comportamento Alimentar , Feminino , Inquéritos Epidemiológicos , Humanos , Laos/epidemiologia , Masculino , Higiene Bucal , Prevalência
9.
Int J Paediatr Dent ; 20(3): 214-21, 2010 May.
Artigo em Inglês | MEDLINE | ID: mdl-20409203

RESUMO

OBJECTIVE: The aim of this study was to compare the clinical and radiographic success of 3Mix and Vitapex((R)) for root canal treatment of pulpally involved primary molars. METHODS: Fifty teeth from 37 healthy children aged 3-8 years with pulpally involved primary molars needing root canal procedures were treated with 3Mix or Vitapex((R)) before restoration with stainless steel crowns. The research employed a prospective single-blinded randomized design. The subjects were followed up clinically and radiographically at 6 and 12 months, respectively. The outcome was compared using a Z-test with a significance level of 0.05. RESULTS: Both groups showed 100% and 96% clinical success at 6 and 12 months, respectively. At 6 months, radiographic success of 3Mix and Vitapex((R)) was 84% and 80%, respectively, and at 12 months, radiographic success of 3Mix and Vitapex((R)) was 76% and 56%, respectively. Considering the radiographic findings at the end of 6 and 12 months, no statistically significant differences were found between the two groups (P = 0.356 and 0.068, respectively). CONCLUSION: 3Mix and Vitapex((R)) can be used as a root canal treatment agent in pulpally involved primary teeth.


Assuntos
Necrose da Polpa Dentária/cirurgia , Materiais Restauradores do Canal Radicular/uso terapêutico , Tratamento do Canal Radicular/métodos , Dente Decíduo/cirurgia , Antibacterianos/administração & dosagem , Hidróxido de Cálcio/química , Hidróxido de Cálcio/uso terapêutico , Criança , Pré-Escolar , Ciprofloxacina/administração & dosagem , Necrose da Polpa Dentária/tratamento farmacológico , Combinação de Medicamentos , Seguimentos , Humanos , Metronidazol/administração & dosagem , Minociclina/administração & dosagem , Dente Molar , Pomadas/administração & dosagem , Pomadas/química , Polietilenoglicóis/administração & dosagem , Propilenoglicol/administração & dosagem , Estudos Prospectivos , Radiografia , Materiais Restauradores do Canal Radicular/química , Silicones/química , Silicones/uso terapêutico , Método Simples-Cego , Dente Decíduo/efeitos dos fármacos , Dente não Vital/diagnóstico por imagem , Resultado do Tratamento
10.
Artigo em Inglês | MEDLINE | ID: mdl-19323021

RESUMO

The aim of the present study was to determine the remineralization effects of xylitol chewing gum containing funoran and calcium hydrogenphosphate on enamel subsurface lesions in humans. The study was a double-blind, randomized, cross-over design, with 4 types of gum: (1) xylitol gum, (2) xylitol gum containing funoran and calcium hydrogenphosphate, (3) sugar gum, and (4) gum base as a control. Seven subjects were instructed to wear removable lingual appliances, with half-slab insets of human enamel containing demineralized subsurface lesions. They were told to chew gum for 20 minutes 4 times per day for 7 days. Upon completion of each treatment the enamel half-slabs were paired with their respective demineralized control half-slabs, embedded, sectioned, and subjected to microradiography and densitometric image analysis, for measurement of the level of remineralization. The mean area of remineralization (deltaZd-deltaZr) and mean percent remineralization (%R) in those chewing xylitol gum containing funoran and calcium hydrogenphosphate were significantly higher than the corresponding values for xylitol gum, sugar gum and gum base. Chewing xylitol gum containing funoran and calcium hydrogenphosphate has a significant effect on the remineralization of initial caries-like lesions of the teeth.


Assuntos
Cariostáticos/uso terapêutico , Esmalte Dentário/efeitos dos fármacos , Edulcorantes/uso terapêutico , Desmineralização do Dente/tratamento farmacológico , Remineralização Dentária/métodos , Xilitol/uso terapêutico , Administração Bucal , Adulto , Fosfatos de Cálcio , Goma de Mascar , Estudos Cross-Over , Densitometria , Método Duplo-Cego , Feminino , Humanos , Masculino , Microrradiografia , Polissacarídeos , Desmineralização do Dente/patologia , Adulto Jovem
11.
Artigo em Inglês | MEDLINE | ID: mdl-19058586

RESUMO

The aim of this study was to determine the levels of secretory IgA (SIgA), pH, flow rates, mutans streptococci (MS) and Candida in saliva of children with rampant caries compared to those caries-free. Thirty children (age 62-123 months) were enrolled and divided into two groups: Group I, children with rampant caries, Group II, caries-free children. The average salivary flow rate was measured from the volume yielded within 5 minutes and the pH was determined using a pH-electrode. Measurement of SIgA was performed using an immunoassay kit. The levels of MS and Candida were determined by culture on Mitis-Salivarius Bacitracin agar and Sabouraud dextrose agar. It was found that children with rampant caries presented with significantly higher levels of salivary SIgA, MS and Candida. However, the mean values for salivary flow rates and pH were similar between the groups. The results reveal that children with rampant caries had significantly higher levels of SIgA, MS and Candida in their oral cavities. This finding tends to support the hypothesis that higher levels of salivary SIgA may reflect a past exposure of the host to cariogenic microorganisms.


Assuntos
Candida/isolamento & purificação , Cárie Dentária/microbiologia , Imunoglobulina A Secretora/análise , Saliva/microbiologia , Saliva/fisiologia , Streptococcus mutans/isolamento & purificação , Criança , Pré-Escolar , Contagem de Colônia Microbiana , Feminino , Humanos , Concentração de Íons de Hidrogênio , Imunoensaio , Masculino , Saliva/química
12.
J Am Dent Assoc ; 136(4): 477-83, 2005 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-15884317

RESUMO

BACKGROUND: The objec tives of this study were to determine microtensile bond strengths of two dentin adhesives and to compare the micromorphological structure of the resin/dentin interface in caries-affected dentin with that of intact dentin. METHODS: The authors randomly divided 40 proximal dentinal carious primary teeth and 40 noncarious anterior primary teeth into two groups (self-etching and total-etching). They used a caries-detecting dye as an indicator of the need to remove the outer carious dentin. The authors restored the teeth with a hybrid resin-based composite. After 24 hours' storage in 37 C water, specimens were sectioned and shaped to form a curved section with a cross-sectional area of 1 square millimeter, then tension was applied until they fractured. The authors prepared the resin/dentin interfaces for the two bonding systems and examined them in 10 occlusal carious and 10 noncarious teeth. STATISTICAL ANALYSIS: The bond strengths for intact and caries-affected dentin within the same group were analyzed via a t test. The authors compared the remaining dentin thickness (RDT) and dentin hardness using analysis of variance and the least significant difference test at the .05 level of significance. RESULTS: The self-etching adhesive demonstrated no statistical difference in bond strength between intact and caries-affected dentin. However, the total-etching adhesive demonstrated different bond strengths for intact and caries-affected dentin. Moreover, the RDT of specimens with intact and caries-affected dentin was not significantly different, whereas the dentin hardness of caries-affected dentin was significantly lower than that of intact dentin. The authors found a thicker hybrid layer in intact and caries-affected dentin of specimens in the total-etching group. CONCLUSION: The adhesives exhibited significantly different bond strengths in intact dentin of primary teeth. However, they exhibited similar bond strengths in caries-affected dentin.


Assuntos
Condicionamento Ácido do Dente/métodos , Colagem Dentária , Cárie Dentária/patologia , Adesivos Dentinários/química , Dentina/ultraestrutura , Dente Decíduo/ultraestrutura , Bis-Fenol A-Glicidil Metacrilato/química , Resinas Compostas/química , Dureza , Humanos , Processamento de Imagem Assistida por Computador , Teste de Materiais , Microscopia Eletrônica de Varredura , Cimentos de Resina/química , Dióxido de Silício/química , Propriedades de Superfície , Resistência à Tração , Zircônio/química
13.
Pediatr Dent ; 26(1): 63-6, 2004.
Artigo em Inglês | MEDLINE | ID: mdl-15080361

RESUMO

PURPOSE: The objectives of this study were to investigate the fluoride levels in plaque and saliva before and after applying fluoride-containing pit and fissure sealants, and compare the fluoride release of 2 types of sealants at the different time intervals. METHODS: Eighteen children ages 6 to 9 years were randomly divided into 2 groups: Group 1--sealant containing fluorosilicate glass (Helioseal-F); and group 2--sealant containing methacryloyl fluoride-methyl methacrylate copolymer (Teethmate-F). Saliva and plaque samples were collected before and after the sealants were placed on their 4 first permanent molars. Fluoride levels were determined using the microdiffusion method. Fluoride concentrations before and after placing the sealants were analyzed by paired t test, and the fluoride concentrations between the 2 sealants were compared by t test, with the level of significance at 0.05. RESULTS: There was no significant difference between salivary fluoride levels before and after sealant placement application in both groups. The plaque fluoride level of Helioseal-F group at 24 hours was significantly higher than the baseline level (P = .03), and was not different afterwards. The plaque fluoride levels after sealant with Teethmate-F were not significantly different when compared to the baseline. However, there were no significant differences between salivary and plaque fluoride levels of the 2 groups at different time intervals. CONCLUSIONS: The groups sealed with sealant containing fluorosilicate glass showed significant increase of plaque fluoride level only at 24 hours after sealant placement.


Assuntos
Cariostáticos/análise , Placa Dentária/química , Fluoretos/análise , Selantes de Fossas e Fissuras/uso terapêutico , Saliva/química , Cariostáticos/química , Cariostáticos/uso terapêutico , Criança , Resinas Compostas/química , Resinas Compostas/uso terapêutico , Índice CPO , Difusão , Fluoretos/química , Fluoretos/uso terapêutico , Seguimentos , Humanos , Concentração de Íons de Hidrogênio , Análise por Pareamento , Selantes de Fossas e Fissuras/química , Fatores de Tempo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...